WO2017197147A3 - Molecular beacon comprising prefabricated components and associated products, processes, and methods of use - Google Patents

Molecular beacon comprising prefabricated components and associated products, processes, and methods of use Download PDF

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Publication number
WO2017197147A3
WO2017197147A3 PCT/US2017/032210 US2017032210W WO2017197147A3 WO 2017197147 A3 WO2017197147 A3 WO 2017197147A3 US 2017032210 W US2017032210 W US 2017032210W WO 2017197147 A3 WO2017197147 A3 WO 2017197147A3
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WO
WIPO (PCT)
Prior art keywords
present
processes
methods
components
labeled
Prior art date
Application number
PCT/US2017/032210
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French (fr)
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WO2017197147A2 (en
Inventor
Robert G. Atkinson
Original Assignee
Atkinson Robert G
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Atkinson Robert G filed Critical Atkinson Robert G
Publication of WO2017197147A2 publication Critical patent/WO2017197147A2/en
Publication of WO2017197147A3 publication Critical patent/WO2017197147A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to improved dissociative molecular beacons constructed from a combination of prefabricated and customized components, and associated products, processes, and methods for their use. The present invention comprises a molecular beacon that is quickly and easily assembled from a kit of parts comprising three components: a pair of target-neutral labeled oligos, each with either a fluorophore or a quencher attached; and a non-labeled target-specific oligo. The labeled oligos of the present invention comprise specific structural ligation site regions and short loop regions. Ligation of the three components forms the molecular beacons of the present invention. In an embodiment, the present invention further comprises a non-labeled target-specific oligo including a toehold region adjacent to a linker region.
PCT/US2017/032210 2016-05-11 2017-05-11 Molecular beacon comprising prefabricated components and associated products, processes, and methods of use WO2017197147A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662334933P 2016-05-11 2016-05-11
US62/334,933 2016-05-11

Publications (2)

Publication Number Publication Date
WO2017197147A2 WO2017197147A2 (en) 2017-11-16
WO2017197147A3 true WO2017197147A3 (en) 2017-12-07

Family

ID=60266813

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/032210 WO2017197147A2 (en) 2016-05-11 2017-05-11 Molecular beacon comprising prefabricated components and associated products, processes, and methods of use

Country Status (1)

Country Link
WO (1) WO2017197147A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040086879A1 (en) * 2001-12-20 2004-05-06 Yingufu Li Tripartite molecular beacons
US20140364333A1 (en) * 2013-03-15 2014-12-11 President And Fellows Of Harvard College Methods for Live Imaging of Cells
WO2015138653A1 (en) * 2014-03-11 2015-09-17 President And Fellows Of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040086879A1 (en) * 2001-12-20 2004-05-06 Yingufu Li Tripartite molecular beacons
US20140364333A1 (en) * 2013-03-15 2014-12-11 President And Fellows Of Harvard College Methods for Live Imaging of Cells
WO2015138653A1 (en) * 2014-03-11 2015-09-17 President And Fellows Of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes

Also Published As

Publication number Publication date
WO2017197147A2 (en) 2017-11-16

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