WO2017150670A1 - Procédé de production de méthane en formation en utilisant des micro-organismes - Google Patents

Procédé de production de méthane en formation en utilisant des micro-organismes Download PDF

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WO2017150670A1
WO2017150670A1 PCT/JP2017/008286 JP2017008286W WO2017150670A1 WO 2017150670 A1 WO2017150670 A1 WO 2017150670A1 JP 2017008286 W JP2017008286 W JP 2017008286W WO 2017150670 A1 WO2017150670 A1 WO 2017150670A1
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methane
activator
microorganism
microorganism group
formation
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PCT/JP2017/008286
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English (en)
Japanese (ja)
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鎌形　洋一
将 坂田
秀幸 玉木
大介 眞弓
聡 玉澤
英治 米林
治男 前田
樹 若山
雅之 五十嵐
典子 大坂
洋 押部
良和 白井
剛史 飯田
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国立研究開発法人産業技術総合研究所
国際石油開発帝石株式会社
東京瓦斯株式会社
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Publication of WO2017150670A1 publication Critical patent/WO2017150670A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • the present invention relates to a method for producing methane in a formation using microorganisms.
  • microbial attack is an attack that can increase efficiency and reduce costs because microorganisms grow and diffuse themselves within the formation and metabolize chemical substances and solvents necessary for enhanced recovery. Expected as a law.
  • microorganisms that have a methane-producing function are collected from within the geological formation and then re-injected into the basement, and then generated from petroleum-based underground resources by the dominant and dominant methane-producing microorganisms.
  • a method for recovering methane has been proposed (see, for example, Patent Document 1).
  • the present invention has been made in view of the above, and an object thereof is to provide a method for producing methane using a microorganism capable of increasing the amount of methane produced in a short period of time.
  • a methane production method using a microorganism capable of increasing the methane production amount in a short period of time is provided.
  • FIG. 4 is a diagram showing the amount of each crude oil component before and after the experiment in Example 2.
  • FIG. 4 is a diagram showing the amount of each crude oil component before and after the experiment in Example 2.
  • FIG. 4 is a diagram showing the amount of each crude oil component before and after the experiment in Example 2.
  • FIG. 5 is a diagram showing the amount of each crude oil component before and after the experiment in Example 3.
  • the method for producing methane using microorganisms includes an activator (microbe group activity) that activates a microorganism group in a formation in which a hydrocarbon-based underground resource and a microorganism group that generates methane from the underground resource exist.
  • the amount of methane produced can be increased in a short period of time by supplying an agent (also referred to simply as an activator).
  • the stratum refers to mud, earth, sand, gravel, ash, or the like, or a combination of these, or a laminate in which a plurality of these layers are combined.
  • artificially formed ones are also included.
  • FIG. 1 is a diagram for explaining a methane production method using microorganisms in the first embodiment.
  • the microorganism group 12 is activated in the underground layer 11 and the formation in which the microorganism group 12 that generates methane from the underground resource 11 exists.
  • Activating agent 100 is supplied.
  • the underground resources 11 are, for example, petroleum-based hydrocarbon resources such as crude oil, shale oil, and sand oil, and coal-based hydrocarbon resources such as lignite, peat, and subbituminous coal, and are buried in the formation.
  • the underground resource 11 may be in any state of solid, liquid, and gas.
  • the underground resource 11 is not particularly limited as long as it contains hydrocarbons that can be used as a substrate by the microorganism group, and is a chain or cyclic aliphatic hydrocarbon, monocyclic or polycyclic aromatic hydrocarbon It is sufficient that one or more of these are included.
  • the aliphatic hydrocarbon may be linear or branched, and may be a saturated hydrocarbon or an unsaturated hydrocarbon.
  • This embodiment is suitable for producing methane using one or more hydrocarbons having 6 to 70 carbon atoms, preferably one or more hydrocarbons having 9 to 34 carbon atoms as a substrate. Further, one or more of alkanes having 6 to 70 carbon atoms, preferably one or more of alkanes having 9 to 34 carbon atoms, more preferably one or more of alkanes having 6 to 40 carbon atoms and alkanes having 9 to 34 carbon atoms. More preferably, it is suitable for producing methane using at least one linear alkane having 9 to 34 carbon atoms as a substrate. Moreover, it is suitable for producing methane using one or more aromatic hydrocarbons having 6 or 7 carbon atoms, preferably toluene as a substrate.
  • Microorganism group 12 inhabits the formation and produces methane from hydrocarbon-based underground resources 11 as described above.
  • the microorganism group 12 includes, for example, a multitude of symbiotic microorganisms, and anaerobically decomposes hydrocarbons such as chain hydrocarbons or cyclic hydrocarbons to reduce organic acids generated in the process. Produces methane.
  • any microorganisms involved in methane production can be used.
  • the microorganism group preferably contains one or more methanogens.
  • the methanogen include archaebacteria such as the Methanobacteria class and the Methanomicrobia class.
  • the methanogen preferably contains at least one of the genus Methanothermobacter, Methanosaeta, and Methanoculleus, and the archaebacteria belonging to the genus Methanothermobacter And at least one archaebacteria belonging to the genus Methanosaeta.
  • the microorganism group preferably contains one or more bacteria belonging to any of the Firmicutes gate, Ca. Atribacteria gate, Ca.
  • Cloacimonetes gate and one species of bacteria belonging to the Firmicutes gate As described above, it is more preferable that one or more bacteria belonging to the Ca. Atribacteria gate and one or more bacteria belonging to the Ca. Cloacimonetes gate are included.
  • the microorganism group may be an uncultured bacterium.
  • the activator (also called nutrient source) 100 may contain vitamins (including vitamin-like substances) or derivatives thereof, preferably water-soluble vitamins or derivatives thereof.
  • vitamins including vitamin-like substances
  • Examples of the activator 100 include Biotin, p-ABA (p-aminobenzoic acid), Pantothenate, Ca salt, Pyridoxine-HCl, Nicotinic acid, Thiamine-HCl ⁇ 2H 2 O, Lipoic (Thioctic) acid, Folic acid, B12 And at least one vitamin selected from the group of vitamins such as Riboflavin.
  • the activator 100 may further contain a metal salt (including a metalloid salt).
  • the activator 100 is, for example, a metal salt such as FeCl 2 , CoCl 2 , MnCl 2 .4H 2 O, ZnCl 2 , H 3 BO 3 , NiCl 2 , AlCl 3 , Na 2 MoO 4 .2H 2 O, CuCl 2, etc. It contains at least one metal salt selected from the group.
  • the activator 100 may contain any one of vitamins and metal salts, or may contain both vitamins and metal salts. In addition, the activator 100 is not restricted to the vitamin group and metal salt group which were illustrated above, If the microorganism group 12 can be activated, it may contain substances other than the above. Further, the activator 100 can include salts such as NH 4 Cl, KH 2 PO 4 , MgCl 2 .6H 2 O, CaCl 2 .2H 2 O, NaCl, and the like.
  • archaea or a medium in which bacteria can be cultured preferably a medium in which anaerobic microorganisms can be cultured, for example, a so-called WS medium can be used.
  • the activator may contain a yeast extract.
  • Yeast extract is extracted by subjecting one or more selected from sake yeast, wine yeast, alcohol yeast, brewer's yeast, baker's yeast, mineral yeast, etc. to self-digestion, enzyme treatment, hot water treatment, etc. Refers to the extracted extract.
  • Yeast extracts include Alanine, Arginine, Aspartic Acid, Cystine, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine (Isoleucine) Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, Valine, Biotin ( Biotin), Choline, Folic acid, Inositol, Nicotinic acid, 4-Aminobenzoic acid, Pantothenic acid, Pyridoxine, Contains one or more of riboflavin and thiamine.
  • one or more of the yeast extracts can be used as the activator 100, or one or more of the yeast extracts and one of the salts other than the above-mentioned vitamins, metal salts, and metal salts. It can also be used by mixing with the above.
  • the production rate of methane using aliphatic hydrocarbons, particularly alkanes, as a substrate can be increased by the microorganism group.
  • the production rate of methane using a straight-chain alkane having 6 to 70 carbon atoms, particularly 9 to 34 carbon atoms as a substrate can be increased.
  • the activator 100 is in a mixed solution containing vitamins and metals as described above, and is pressed into the formation through a conduit, for example, by a pump or the like, and supplied together with the underground resources 11 to the microorganism group 12 existing in the formation.
  • the microorganism group 12 is activated and the production of methane from the underground resource 11 is promoted.
  • the methane 13 generated from the underground resource 11 by the microorganism group 12 is recovered on the ground through a conduit provided so as to communicate with the underground resource 11 from the ground, for example.
  • the methane production method using microorganisms in the first embodiment by supplying the activator 100 to the microorganism group 12 that produces methane from the underground resource 11 in the formation, It becomes possible to increase the amount of methane produced in a short period of time by activation.
  • the activator 100 is supplied in the formation existing in the ground, but the formation does not necessarily exist underground. If an environment capable of producing methane can be prepared by underground resources and microbial groups capable of producing methane from the underground resources, even if natural strata raised above the ground are used, artificially formed strata are used. Also good.
  • An environment in which methane can be generated by an underground resource and a group of microorganisms that can generate methane from the underground resource is, for example, in an anaerobic atmosphere at a temperature of 10 to 120 ° C. and a pressure of 0.1 to 70 MPa. be able to. The temperature is more preferably 35 to 75 ° C. The pressure is more preferably 3 to 15 MPa.
  • the microorganism group is activated to produce methane in a short period of time.
  • the amount can be increased.
  • a group of microorganisms that generate methane from underground resources is supplied into a formation where there are no microorganism groups that generate methane from underground resources.
  • microbial groups collected from other formations are supplied into the formations that are small in quantity or low in activity and do not generate enough methane.
  • methane can be generated in a short period of time by supplying microbial groups into the formation where underground resources exist.
  • the activator that activates the microorganism group is insufficient in the formation, the amount of methane produced can be increased in a short period of time by supplying the activator together with the microorganism group into the formation. .
  • FIG. 2 is a diagram for explaining a methane production method using microorganisms in the second embodiment.
  • an activator that activates the microorganism group 210 and, if necessary, the microorganism group 210 in the formation in which the underground resource 21 exists. 220 is supplied.
  • the underground resources 21 are, for example, petroleum-based hydrocarbon resources such as crude oil, shale oil, and sand oil, and coal-based hydrocarbon resources such as lignite, peat, and subbituminous coal, and are buried in the formation.
  • the underground resource 21 is the same as that exemplified in the first embodiment.
  • the microorganism group 210 generates methane from the hydrocarbon-based underground resource 21 as described above.
  • the microorganism group 210 includes, for example, a large number of symbiotic microorganisms, and generates methane from the underground resource 21 by anaerobically decomposing organic substances and reducing organic acids generated in the process.
  • any microorganisms involved in the production of methane can be used.
  • the microorganism group 210 is collected from, for example, a formation different from the formation in which the underground resource 21 exists, and after being cultured in a culture solution containing a substrate (crude oil), water, and an activator that activates the microorganism, The resource 21 is supplied into the formation where it exists.
  • This culture can be performed outside the formation.
  • the microorganism group 210 is cultured in a culture tank maintained at an temperature of 10 to 120 ° C. under an anaerobic condition as in the formation, and at a normal pressure or a pressurized environment, for example, 0.1 to 70 MPa. In this state, it is pressed into the formation through a conduit by a pump or the like and supplied to the underground resource 21.
  • the microorganism group 210 cultured in this way preferably contains, for example, one or more methanogens.
  • the methanogen include archaebacteria such as the Methanobacteria class and the Methanomicrobia class.
  • the methanogen preferably contains at least one of the genus Methanothermobacter, Methanosaeta, and Methanoculleus, and the archaebacteria belonging to the genus Methanothermobacter And at least one archaebacteria belonging to the genus Methanosaeta.
  • the microorganism group preferably contains one or more bacteria belonging to any of the Firmicutes gate, Ca. Atribacteria gate, Ca.
  • Cloacimonetes gate and one species of bacteria belonging to the Firmicutes gate As described above, it is more preferable that one or more bacteria belonging to the Ca. Atribacteria gate and one or more bacteria belonging to the Ca. Cloacimonetes gate are included.
  • the microorganism group may be an uncultured bacterium.
  • the first activator depends on the type of microorganism contained in the microorganism group. Components contained in the activator exemplified in the embodiment can be used.
  • the activator 220 may be pressed into the formation together with the microorganism group 210. By supplying the activator 220, it becomes possible to activate the microorganism group 210 supplied to the underground resource 21 and increase the amount of methane produced in a short period of time.
  • the activator 220 is the same as the activator 100 exemplified in the first embodiment, and includes vitamins, metals, and the like that activate the microorganism group 210.
  • the activator 220 is pressed into the formation with a culture solution containing the microorganism group 210 by a pump or the like, for example, in a mixed solution containing vitamins and metal salts, and supplied to the underground resource 21.
  • the microorganism group 210 is activated and the amount of methane produced increases in a short period of time.
  • the methane 22 produced by the microorganism group 210 is collected on the ground through a conduit provided so as to communicate with the underground resource 21 from the ground.
  • the microorganism group 210 that generates methane from the underground resource 21 is supplied into the formation, so that the microorganism group 210 can generate methane from the underground resource 21. Can be generated in a short period of time.
  • the microorganism group 210 can be activated and the amount of methane produced can be increased in a short period of time.
  • microbes collected and cultured from a predetermined formation may be supplied again into the same formation, it may be preferable to use a group of microorganisms collected from a different formation.
  • microbes collected from the same strata have already undergone geological periods and subterranean resources that match their metabolic pathways have already been degraded, and other metabolites taken from strata different from crude oil and oil / water reservoirs. This is because there is a case where currently remaining underground resources cannot be decomposed without using a microorganism group having a route.
  • the amount of methane produced can be increased in a short period of time by supplying the microorganism group or the microorganism group and the activator to the underground resource. It becomes possible to make it.
  • Example 1 100 ml of crude oil (collected from oil fields in Japan), 300 ml of oil reservoir (collected from oil fields in Japan), 250 ml of activator (described later), and 5 ⁇ l of toluene labeled with stable isotope 13C in 1 L stainless steel Placed in a container. In the container, 250 ml of a porous material was previously placed.
  • the atmosphere inside the container was an anaerobic atmosphere, and the pressure and temperature inside the container were kept at 5 MPa and 55 ° C., respectively.
  • the atmosphere, pressure and temperature were the same conditions as the environment in the formation from which the crude oil and oil reservoir water were collected.
  • acetic acid includes acetate ion and acetate in addition to acetic acid.
  • a gas chromatograph and a high performance liquid chromatograph were used.
  • the stable isotope ratio (13C / 12C) of carbon in the produced methane was measured using a mass spectrometry gas chromatograph (GCMS).
  • the stable isotope ratio (atm%) was determined by the following formula.
  • Example 1 The crude oil and oil reservoir water used in Example 1 are collected from the same oil field and contain a group of microorganisms that produce methane from crude oil.
  • the bacteria of the Firmicutes, Ca.Atribacteria, Ca.Cloacimonetes, Methanothermobacter, and Methanosaeta was included.
  • WS medium was used as an activator. Specifically, vitamins (Biotin, p-ABA (p-aminobenzoic acid), Pantothenate, Ca salt, Pyridoxine-HCl, Nicotinic acid, Thiamine-HCl 2H 2 O, Lipoic (Thioctic) acid, Folic acid, B12 , Riboflavin) and metal salt group (FeCl 2 , CoCl 2 , MnCl 2 ⁇ 4H 2 O, ZnCl 2 , H 3 BO 3 , NiCl 2 , AlCl 3 , Na 2 MoO 4 ⁇ 2H 2 O, CuCl 2 ) It is. In addition to the above-mentioned vitamin group and metal salt group, NH 4 Cl, KH 2 PO 4 , MgCl 2 .6H 2 O, CaCl 2 .2H 2 O, and NaCl are added.
  • vitamins Biotin, p-ABA (p-aminobenzoic acid), Pantoth
  • Example 1 The experiment was carried out in the same manner as in Example 1 except that the amount of oil layer water was changed and no toluene labeled with an activator and a stable isotope was added.
  • the crude oil and oil reservoir water used in Comparative Example 1 are the same as the crude oil and oil reservoir water used in Example 1, and contain a group of microorganisms that produce methane from crude oil.
  • FIG. 3 is a graph showing changes in the amount of methane produced and the amount of acetic acid in the container in Example 1 and Comparative Example 1.
  • the horizontal axis represents the number of days elapsed from the start of measurement
  • the vertical axis represents the concentration of methane and the concentration (mM) of acetic acid in the container.
  • FIG. 4 is a diagram showing the measurement results of the stable isotope ratio of carbon in the produced methane in Example 1.
  • the horizontal axis represents the number of days elapsed from the start of measurement
  • the vertical axis represents the stable isotope ratio of carbon in the produced methane.
  • Example 1 As shown in FIG. 3, in Example 1, the concentration of methane increased to about 4 mM between about 60 days after the start of measurement, and further increased by about 2 mM after about 60 days. The concentration of acetic acid increased to more than 2 mM between the start of measurement and about 20 days, but thereafter decreased below the lower limit of quantification.
  • Comparative Example 1 In contrast, in Comparative Example 1, a small amount of methane was produced after about 150 days. However, the amount of methane produced in Comparative Example 1 was about 0.4 mM or less, which was a very small amount. In Comparative Example 1, the amount of acetic acid in the container was almost zero.
  • Example 1 it can be seen that by adding the activator, the microbial group was remarkably activated with respect to the comparative example in which no activator was added, and the amount of methane produced increased in a short period of time. .
  • the amount of acetic acid in the container increased from the start of measurement to about 20 days. This is because the microorganism group in which components having low carbon chains such as easily degradable volatile fatty acids are activated. This is considered to be caused by decomposition into acetic acid or a derivative thereof.
  • methane corresponding to a concentration increase of about 4 mM was generated in the period from the start of measurement to about 60 days, and this period was almost the same as the period in which the amount of acetic acid in the container decreased. It matches. Therefore, it is considered that methane produced in a period of up to about 60 days is mainly derived from decomposition of volatile fatty acids and the like.
  • Example 1 methane corresponding to an increase in concentration of about 2 mM was further generated after about 60 days. As shown in FIG. 4, the stable isotope ratio of carbon in the produced methane gradually increases after about 60 days and reaches about 2 atm% after about 280 days. The generated methane is thought to originate mainly from the decomposition of toluene in crude oil and other crude oil components.
  • Example 2 In Example 2, the experiment was performed in the same manner as in Example 1 except that the amount of the activator was changed and a culture solution containing a microorganism group was additionally added.
  • 1L stainless steel in which 250 ml of sea sand was placed in 100 ml of crude oil, 300 ml of oil reservoir water, 260 ml of activator, 40 ml of culture solution containing microorganisms, and 5 ⁇ l of toluene in which all the constituent carbons were labeled with stable isotopes 13C. Placed in a container. In an anaerobic atmosphere, the pressure and temperature in the container were maintained at 5 MPa and 55 ° C., respectively, and the amount of methane produced, the amount of acetic acid in the container, and the stable isotope ratio of carbon in the produced methane were measured.
  • Example 2 The crude oil and oil reservoir water used in Example 2 are the same as those used in Example 1, both of which were collected from the same oil field.
  • the activator is the same as the activator used in Example 1, including vitamins (Biotin, p-ABA (p-aminobenzoic acid), Pantothenate, Ca salt, Pyridoxine-HCl, Nicotinic acid, Thiamine).
  • the microorganism group in the culture solution containing the microorganism group used in this example was collected from an oil field different from the oil field from which the crude oil and the oil reservoir water were collected, and the crude oil (substrate), water, and microorganism activation substance.
  • the liquid containing the lysate one cultured in an anaerobic environment at a pressure of 5 MPa and a temperature of 55 ° C was used.
  • the bacteria include the bacteria of the Firmicutes, Ca.Atribacteria, Ca.Cloacimonetes, Methanothermobacter, and Methanosaeta It was.
  • Example 2 Experiments were conducted in the same manner as in Example 2 except that the amounts of the oil reservoir water and the activator were changed and the culture solution containing the microorganism group and toluene labeled with a stable isotope were not added.
  • 100 ml of crude oil, 400 ml of oil reservoir water, and 300 ml of activator were placed in a 1 L stainless steel container, and the pressure and temperature in the container were maintained at 5 MPa and 55 ° C., respectively, and the amount of methane produced and the amount of acetic acid in the container were measured.
  • the crude oil, oil reservoir water, and activator used in Comparative Example 2 are the same as the crude oil, oil reservoir water, and activator used in Example 2.
  • FIG. 5 is a graph showing changes in the amount of methane produced and the amount of acetic acid in Example 2 and Comparative Example 2.
  • the horizontal axis represents the number of days elapsed from the start of measurement
  • the vertical axis represents the methane concentration and the acetic acid concentration (mM).
  • FIG. 6 is a graph showing the measurement results of the stable isotope ratio of carbon in the produced methane in Example 2.
  • the horizontal axis represents the number of days elapsed from the start of measurement, and the vertical axis represents the stable isotope ratio of carbon in the produced methane.
  • Example 2 the amounts of aliphatic and aromatic hydrocarbons were measured before and after the experiment, that is, at the time when 0 day had elapsed (before the start of the reaction) and at the time when 750 days had elapsed, using a gas chromatograph.
  • FIG. 7 shows the results for straight chain alkanes having 9 to 25 carbon atoms
  • FIG. 8 shows the results for monocyclic aromatic hydrocarbons
  • FIG. 9 shows the results for polycyclic aromatic hydrocarbons. Show.
  • Example 2 the methane concentration increased to about 10 mM from the start of measurement to about 40 days, and remained almost unchanged from about 40 days to about 250 days. .
  • the methane concentration further increased by about 40 mM between about 250 days and about 750 days.
  • the concentration of acetic acid decreased by about 5 mM between the start of measurement and about 20 days, and thereafter became lower than the lower limit of quantification.
  • Comparative Example 2 On the other hand, in Comparative Example 2, almost no methane was produced between about 50 days from the start of measurement and an increase in concentration of about 10 mM was observed from about 50 days to about 200 days. Thereafter, there was no change in the concentration of methane between about 200 days and about 750 days. In Comparative Example 2, the acetic acid concentration increased to about 10 mM from the start of measurement to about 20 days and decreased from about 20 days to about 200 days. The acetic acid concentration was below the lower limit of quantification after about 200 days.
  • toluene decreased from about 45 mM to about 7 mM after a predetermined period, but there was almost no change in other components.
  • Example 2 by supplying the microorganism group and the activator, methane generation from crude oil by the microorganism group activated by the activator is performed, and the amount of methane generated increases in a short period of time. I understand that.
  • methane corresponding to an increase in concentration of about 10 mM was generated in the period from the start of measurement to about 40 days, and this period was almost the same as the period in which the amount of acetic acid in the container decreased. It matches. Therefore, it is considered that methane produced in a period of up to about 40 days is mainly derived from decomposition of volatile fatty acids and the like.
  • Example 2 methane corresponding to a concentration increase of about 40 mM was further generated between about 250 days and about 750 days.
  • the stable isotope ratio of carbon in the generated methane has increased to about 1.3 atm% after about 290 days (Fig. 6), and a decrease in toluene in crude oil components has been observed before and after the experiment.
  • Fig. 8 methane produced after about 250 days is considered to originate mainly from the decomposition of toluene in crude oil because it almost matches the methane production calculated stoichiometrically from the reduction in toluene. It is done.
  • the amount of acetic acid in the container is increased from the start of measurement to about 20 days. This is considered to be caused by the fact that a component having a low carbon chain such as an easily decomposable volatile fatty acid was decomposed into acetic acid and / or a derivative thereof by an activated microorganism group. Further, in Comparative Example 2, methane corresponding to an increase in concentration of about 10 mM was generated in the period from about 50 days to about 200 days, and this period substantially coincides with the period in which the amount of acetic acid in the container decreased. I'm doing it. Therefore, it is considered that methane produced in a period from about 50 days to about 200 days is mainly derived from decomposition of volatile fatty acids and the like.
  • Example 2 the activator was supplied to the crude oil together with the microorganism group. However, if there is a substance that activates the microorganism in the ground, the activator is prepared by subtracting the existing amount and supplied. May be. Or you may supply only a microorganism group, without giving an activator. Also in this case, the supplied microorganism group generates methane from crude oil, so that the amount of methane generated increases in a short period of time.
  • Example 3 The experiment was performed in the same manner as in Example 1 except that the amount of oil layer water was changed, the composition and amount of the activator were changed, and toluene labeled with a stable isotope was not added.
  • Activators include vitamins (Biotin, p-ABA (p-aminobenzoic acid), Pantothenate, Ca salt, Pyridoxine-HCl, Nicotinic acid, Thiamine-HCl ⁇ 2H 2 O, Lipoic (Thioctic) acid, Folic acid, B12, Riboflavin) and metal salts (FeCl 2 , CoCl 2 , MnCl 2 ⁇ 4H 2 O, ZnCl 2 , H 3 BO 3 , NiCl 2 , AlCl 3 , Na 2 MoO 4 ⁇ 2H 2 O, CuCl 2 ) NH 4 Cl, KH 2 PO 4 , MgCl 2 .6H 2 O, CaCl 2 .2H 2 O, and NaCl are added to the aqueous solution, and 5 ml of yeast extract is added.
  • vitamins Biotin, p-ABA (p-aminobenzoic acid), Pantothenate, Ca salt, Pyridox
  • Yeast extracts include Alanine, Arginine, Aspartic Acid, Cystine, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine (Isoleucine) Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, Valine, Biotin ( Biotin), Choline, Folic acid, Inositol, Nicotinic acid, 4-Aminobenzoic acid, Pantothenic acid, Pyridoxine, It contained riboflavin and Thiamine.
  • the concentration of methane and the concentration of acetic acid in the container were measured at arbitrary time intervals of several days to several tens of days over about 750 days.
  • the methane concentration was 0 mM, but after 124 days of culture, it was recorded that the methane concentration was 190 mM.
  • the concentration in the container was measured in the same manner as in Example 2 for a plurality of aliphatic hydrocarbons before and after the experiment, that is, at the time when 0 day had elapsed (before the start of reaction) and at the time when 750 days had elapsed.
  • the measurement results are shown in FIG.
  • the graph in FIG. 10 shows the results for a linear alkane (n-alkane) having 9 to 34 carbon atoms. After the experiment (after 750 days had elapsed), it was found that the concentration of all linear alkanes to be measured was lower than the concentration before the experiment (0 days).
  • Example 3 it was found that by using an activator containing a yeast extract, the microorganism group can produce methane in a short period of time using an aliphatic hydrocarbon as a substrate.

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  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

La présente invention décrit un procédé de production de méthane en utilisant des micro-organismes, avec lequel il est possible d'accroître la quantité de méthane produit sur une courte durée. La présente invention décrit un procédé de production de méthane en utilisant des micro-organismes, le procédé étant caractérisé en ce qu'il présente une étape d'alimentation d'activateur pour alimenter un activateur afin d'activer la communauté microbienne dans une formation dans laquelle des ressources souterraines à base d'hydrocarbure et une communauté microbienne qui produit du méthane à partir des ressources souterraines sont présentes.
PCT/JP2017/008286 2016-03-04 2017-03-02 Procédé de production de méthane en formation en utilisant des micro-organismes WO2017150670A1 (fr)

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JP2016042738A JP2019071791A (ja) 2016-03-04 2016-03-04 微生物を用いた地層内メタン生成方法
JP2016-042738 2016-03-04

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WO2017150670A1 true WO2017150670A1 (fr) 2017-09-08

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015052806A1 (fr) * 2013-10-10 2015-04-16 中外テクノス株式会社 Procédé de production d'hydrogène dans le sol et procédé de production de méthane dans le sol

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015052806A1 (fr) * 2013-10-10 2015-04-16 中外テクノス株式会社 Procédé de production d'hydrogène dans le sol et procédé de production de méthane dans le sol

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HAJIME KOBAYASHI: "Molecular basis of oildegrading methanogenic microbial symbiotic system indigenous to petroleum reservoir", GRANTS-IN-AID FOR SCIENTIFIC RESEARCH KENKYU SEIKA HOKOKUSHO, 31 July 2013 (2013-07-31), pages 3, XP055602813, Retrieved from the Internet <URL:https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-21880014/21880014seika/> [retrieved on 20170511] *
MASAYUKI IKARASHI ET AL: "A method for rapid microbial conversion of the residual oil into methane in subsurface oil reservoir", 46TH PETROLEUM-PETROCHEMICAL SYMPOSIUM OF JPN. PETROL. INST. (KYOTO CONVENTION, 1 November 2016 (2016-11-01), pages 364 - 365, XP055602809, Retrieved from the Internet <URL:https://www.jstage.jst.go.jp/article/sekiyu/2016f/0/2016f_247/_article> [retrieved on 20170511] *
MASAYUKI IKARASHI ET AL: "Can microbes really convert residual oil into methane in subsurface oil reservoir?", 45TH PETROLEUM-PETROCHEMICAL SYMPOSIUM OF JPN. PETROL. INST, 5 January 2016 (2016-01-05), pages 31 - 32, XP055602815, Retrieved from the Internet <URL:https://www.jstage.jst.go.jp/article/sekiyu/2015f/0/2015f_12/_article> [retrieved on 20170511] *

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