WO2017129988A1 - Continuous complexation of active pharmaceutical ingredients - Google Patents

Continuous complexation of active pharmaceutical ingredients Download PDF

Info

Publication number
WO2017129988A1
WO2017129988A1 PCT/GB2017/050210 GB2017050210W WO2017129988A1 WO 2017129988 A1 WO2017129988 A1 WO 2017129988A1 GB 2017050210 W GB2017050210 W GB 2017050210W WO 2017129988 A1 WO2017129988 A1 WO 2017129988A1
Authority
WO
WIPO (PCT)
Prior art keywords
solution
process according
cyclodextrin
complex
reactor
Prior art date
Application number
PCT/GB2017/050210
Other languages
French (fr)
Inventor
Hugo LISBOA
Márcio TEMTEM
João VINCENTE
Filipa SANTOS
Original Assignee
Hovione Scientia Limited
Turner, Craig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU2017211520A priority Critical patent/AU2017211520B2/en
Priority to CN201780013795.1A priority patent/CN108778338B/en
Priority to DK17707096.8T priority patent/DK3400017T3/en
Priority to ES17707096T priority patent/ES2785625T3/en
Priority to PL17707096T priority patent/PL3400017T3/en
Priority to CA3011820A priority patent/CA3011820C/en
Application filed by Hovione Scientia Limited, Turner, Craig filed Critical Hovione Scientia Limited
Priority to JP2018539297A priority patent/JP7038057B2/en
Priority to US16/073,469 priority patent/US11759529B2/en
Priority to BR112018015481-4A priority patent/BR112018015481B1/en
Priority to EP17707096.8A priority patent/EP3400017B1/en
Publication of WO2017129988A1 publication Critical patent/WO2017129988A1/en
Priority to IL260766A priority patent/IL260766B/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6949—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes
    • A61K47/6951—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes using cyclodextrin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/716—Glucans
    • A61K31/724—Cyclodextrins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A61K47/40—Cyclodextrins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
    • A61K9/145—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A61K9/1617—Organic compounds, e.g. phospholipids, fats
    • A61K9/1623—Sugars or sugar alcohols, e.g. lactose; Derivatives thereof; Homeopathic globules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1682—Processes
    • A61K9/1694—Processes resulting in granules or microspheres of the matrix type containing more than 5% of excipient

Definitions

  • the present invention relates generally to complexes of active pharmaceutical ingredients (API's), particularly to those based on one or more cyclodextrins, and to improved processes for the provision of such complexes.
  • API's active pharmaceutical ingredients
  • Cyclodextrins are cyclic oligosaccharides compounds of 6, 7 or 8 repetitive units with an increasing hydrophobic cavity diameter. They can be designated respectively by alpha, beta or gamma depending on the number of repetitive units. Cyclodextrins can be, for example, prepared by enzymatic conversion of starch. Their external groups are hydrophilic and can be chemically modified, while their internal cavity can be hydrophobic which allows the inclusion of lipophilic molecules, for example, active pharmaceutical ingredients with reduced aqueous solubility.
  • hydrophilic polymers are used to increase the concentration of the complex formed. This makes the process more expensive, and free polymer will be present in the final powder which is considered to be disadvantageous.
  • the formation and dissociation rate of the cyclodextrin/API complexes is similar, and their half-life is only a few thousands of second in solution, meaning that said complexes are continuously being formed and dissociated (Lima Guedes et al., 2008).
  • An objective of the present invention is to provide an improved process for complexation between cyclodextrins and active pharmaceutical ingredients without the disadvantages of the known processes and where it is possible to obtain the desired complexes in less time, with higher complex concentration, with lower solvent quantities, with the possibility of increasing the ratio of active pharmaceutical ingredient / cyclodextrin and where the energy input occurs in a spontaneous way. All these factors contribute to the overall economy of the process.
  • the present invention presents a continuous process of obtaining complexes consisting of a cyclodextrin and active pharmaceutical ingredients dissolved in suitable solvents using a microfluidization system with high levels of mixing and spontaneous heat generation that surprisingly allows complexes to be obtained in less time with high complexation efficiency and that can optionally be followed by spray drying to obtain a solid material.
  • the present invention describes a continuous or not continuous process, in particular to obtain cyclodextrin/active pharmaceutical ingredient(s) complexes in reduced time.
  • a process for preparing a complex of at least one cyclodextrin and at least one active pharmaceutical ingredient comprising the steps of: a. Preparing a first solution (solution A) comprising at least one cyclodextrin and at least one solvent;
  • solution B comprising at least one dissolved, partially dissolved or suspended API
  • the present invention thus relates to the increased bioavailability of active pharmaceutical ingredients (API) preferably by the continuous formation of a complex of cyclodextrin and the active pharmaceutical ingredients using a microfluidization system. Alternatively the process can also be combined with spray drying to isolate the complex in powder form. More specifically, the present invention is related to a continuous or dis- continuous complexation process where a first solution of cyclodextrin is combined by microfluidization with a second solution, partial solution, or suspension of an active pharmaceutical ingredient (API)or the solid form of an active pharmaceutical ingredient (API), the first and second solutions (or partial solution, suspension, solid etc.) being either miscible or immiscible.
  • API active pharmaceutical ingredients
  • the present invention also concerns the time reduction to obtain such a complex and the combination of a complexation step followed by a spray drying step which can make the present invention a continuous process with high complexation efficiency, where the obtained powder comprising the complex has high drug loading.
  • the present invention thus represents a benefit in terms of reduction of process times.
  • the process may be a batch process or a continuous process.
  • Solution A preferably comprises at least one cyclodextrin comprising any substitution group and any cavity size, and/or a polymeric pharmaceutical excipient.
  • the cyclodextrin may, for example, be one or more of a-cyclodextrin, ⁇ -cyclodextrin, ⁇ - cyclodextrin, sulfobutylether-beta-cyclodextrin, hydropropyl - beta- cyclodextrin, methyl-beta-cyclodextrin and/or maltosyl - beta - cyclodextrin.
  • a particularly preferred cyclodextrin is sulfobutylether - beta -cyclodextrin.
  • Solution A preferably comprises at least one of the following solvents: water, ethanol, methanol, isopropanol, dichloromethane acetone, methyl ethyl ketone, tetrahydrofuran, di-methyl sulfoxyde, di-methyl formaldehyde, or di-methyl acetamide.
  • a polymeric pharmaceutical excipient may be present in solution A, for example at a concentration of from 1 to 20%(w/w).
  • the pH of solution A typically ranges from 1 to 14, and a preferred pH is 6 to 8.
  • Solution B preferably comprises at least one API dissolved in one or more solvents, partially dissolved in one or more solvents, or in suspension in one or more solvents.
  • the API or its pharmaceutically acceptable derivative has at least one functional group selected from: thiother, alcohol, thiol, aldehyde, ketone, thioketone, nitrate ester, phosphate ester, thiophosphate ester, ester, thioester, sulfate ester, carboxylic acid, phosphonic acid, sulfonic acid, amide, primary amine, secondary amine, ammonia, tertiary amine, imine, thiocyanate, cyanamide, oxime, nitrile, diazo, organohalide, nitro, S-heterocyclic ring, thiophene, N-heterocyclic ring, pyrrole, O- heteocyclic ring, furan, epoxide, peroxide, hydrox
  • Examples of preferred APIs include, but are not limited to, poorly soluble active compounds.
  • Examples of poorly soluble compounds include, but are not limited to: antifungal agents like itraconazole or a related drug, such as fluoconazole, terconazole, ketoconazole and saperconazole; anti-infective drugs, such as griseofulvin and related compounds (e.g. griseoverdin); anti malaria drugs (e.g.
  • ibuprofen sterols or steroids; drugs from the group comprising danazol, acyclovir, dapsone, indinavir, nifedipine, nitrofurantion, phentytoin, ritonavir, saquinavir, sulfamethoxazole, valproic acid, trimethoprin, acetazolamide, azathioprine, iopanoic acid, nalidixic acid, nevirapine, praziquantel, rifampicin, albendazole, amitrptyline, artemether, lumefantrine, chloropromazine, ciprofloxacin, clofazimine, efavirenz, iopinavir, folic acid, glibenclamide, haloperidol, ivermectin, mebendazole, niclosamide
  • Solution B preferably comprises at least one of the following solvents: water, ethanol, methanol, isopropanol, dichloromethane, acetone, methyl ethyl ketone, tetrahydrofuran, di-methyl sulfoxyde, di-methyl formaldehyde, or di-methyl acetamide.
  • the one or more solvents in solution B can be the same or different from those solvents in Solution A, and if different said solvents can be miscible or immiscible with each other.
  • the concentration of API in solution B preferably ranges from 0.01 to 100% (w/w).
  • Solution B is typically prepared using a jacketed reactor with agitation, by adding at least one solvent to the reactor and at least one API.
  • the order of addition of the components is not restricted and can be any way around.
  • a solid API may be fed continuously, for example, by a positive displacement equipment, such as a screw-based feeding system or a pneumatic system.
  • thee solid API is fed continuously, preferably using a hopper.
  • the microfludization preferably occurs in a system comprising at least one intensifier pump that can mix and displace a liquid mixed with another miscible or immiscible liquid, or alternatively a liquid mixed with a solid compound, into at least one micro- channel or micro-reactor. Two or more micro-channels or micro-reactors may be used if desired.
  • the micro-channel or micro-reactor is a continuous flow reactor, suitably with a lateral dimension of equal to or below 1000 microns.
  • the lateral dimension may be equal to or below 200 microns.
  • the feed flow ratio of solution A to solution B or solid API ranges from about 0.1 to about 10 kg/kg.
  • the feed flow ratio of solution A and the amount of API added can range from 0.01 to 10 kg/kg.
  • the process temperature can be any suitable temperature, bearing in mind the components involved, and it suitably ranges from 0°C to 90°C.
  • Isolation of the complex from the complex solution and/or suspension may be by any means, but preferably comprises one or more drying techniques.
  • One particularly suitable drying technique comprises spray drying.
  • isolation of the complex from the complex solution and/or suspension may be a batch process or continuous process.
  • the complex is isolated in powdered form.
  • the complexes produced by the method of the invention may be used in medicine, for example as part of a pharmaceutical composition.
  • the invention thus provides this use, or alternatively a process characterized in that the obtained complex in solution and/or suspension or powdered form is used for pharmaceutical purposes.
  • the invention provides a process for preparing a complex of at least one cyclodextrin and at least one active pharmaceutical ingredient comprising the steps of a) Preparation of Solution A with at least one cyclodextrin in one or more solvents;
  • Solution B Preparation of Solution B with at least one dissolved or suspended API.
  • the solvent can be the same or different than Solution A, and if different can be miscible or immiscible.
  • the active pharmaceutical ingredient(s) are added in the solid state by means of a positive displacement equipment, preferably a hopper and a screw feed;
  • the present invention also presents a method to obtain powder with higher concentration of the active pharmaceutical ingredient complexed with the cyclodextrin in less time than other known processes.
  • the present invention allows the continuous production of cyclodextrin / active pharmaceutical ingredient complex and its continuous isolation using spray drying.
  • the present invention comprises a microfluidization system.
  • the microfluidization system typically consists of an intensifier pump that can mix and displace a liquid mixed with another miscible or immiscible liquid, or alternatively a liquid mixed with a solid compound, into a combination of micro-channels.
  • a micro-channel is typically a continuous flow reactor with lateral dimensions less of 1000 ⁇ .
  • the present invention uses two different setups:
  • Setup A comprising: a) A reactor to prepare Solution A;
  • Figure 1 shows a schematic flowchart of the present invention process.
  • Figure 4 shows A representation of the concentration of complex formed vs time. Dotted line represents the example 1 method. The dashed line represents example 2 method. The solid line represents the example 3 method.
  • the present invention presents a continuous or not continuous process for the complexation of a cyclodextrin and at least one active pharmaceutical ingredient optionally followed by a drying step.
  • the drying step can, for example, be a spray drying process, but other suitable drying processes may be used.
  • the present invention presents a continuous or not continuous (i.e. dis-continuous) process for the complexation of a cyclodextrin and at least one active pharmaceutical ingredient by mixing a solution A comprising at least one dissolved cyclodextrin and solution B comprising at least one dissolved or suspended API or alternatively the solid API fed by a positive displacement equipment, preferably a hopper equipped with a screw feed, forming stream B.
  • a positive displacement equipment preferably a hopper equipped with a screw feed
  • the obtained complex solution is continuously or not continuously dried producing the complex of cyclodextrin and active pharmaceutical ingredient in the powder form.
  • Solution A comprises a solution with one or more solvents, that are preferably chosen from, water, ethanol, methanol, isopropanol, dichloromethane, acetone, Methyl Ethyl Ketone, TetraHydroFuran, Di-methyl Sulfoxyde, Di-methyl Formaldehyde, or Di-methyl Acetamide.
  • the solution A also comprises one or more dissolved cyclodextrins or substituted cyclodextrins.
  • the concentration of the one or more cyclodextrins is preferably from 1 %(w/w) to 50%(w/w).
  • Solution A has a pH value ranging from 1 to 14, preferably of from 6 to 8.
  • Solution A can be prepared, for example, using a jacketed reactor with agitation, by adding solvent to the reactor and by adding cyclodextrin(s) to the same reactor, followed by the pH adjustment. The order of addition is nor restricted and can be done either way around.
  • Solution B comprises a solution or suspension using one or more solvents, which are preferably chosen from, water, ethanol, methanol, isopropanol, dichloromethane, acetone, Methyl Ethyl Ketone, TetraHydroFuran, Di-methyl Sulfoxyde, Di-methyl Formaldehyde, or Di-methyl Acetamide.
  • Solution B also comprises at least one active pharmaceutical ingredient (API) which may be dissolved, partially dissolved or in suspension in the solvent.
  • the API is preferably present at a concentration ranging from 0.01 % (w/w) to 100%(w/w).
  • active pharmaceutical ingredients in their solid form can be used.
  • Solution B may, for example, be prepared using a jacketed reactor with agitation where, the solvent or solvents and the at least one Active pharmaceutical ingredient are added to the reactor. The order of addition is not restricted and can be done either way around.
  • a feed flow of Solution A to the microfluidization system can range from 0.1 to 10 times the Solution B feed flow to the microfluidization system. Ratios of 0.5 to 5, or 0.5 to 3, or 1 to 3 may also be used (as per above definition).
  • microfluidization is a term understood by those skilled in this field.
  • microreaction refers to a technology that involves physical and/or chemical reactions within microreactors, micromixers, microchannels or any other component comprised within the microfluidic field.
  • microfluidization encompasses continuous fluid processing through these microchannels, involving high shear, cavitation and uniform mixing in the meso- and micromixing range.
  • the microfluidization system comprises, for example, an intensifier pump that draws stream A and solution B or the at least one API in the solid form, continuously or not (i.e.
  • the pump creates pressures ranging from 1 to 1500 Bar (can we give some preferred sub-ranges?), and the mixed liquid temperature is spontaneously increased which represents an increase in efficiency as less energy is spent drying the mixed liquid.
  • a solution with a high concentration of the cyclodextrin / active pharmaceutical ingredient complex is obtained in much less time than reported previously.
  • the obtained solution can, if desired, be continuously or not continuously fed into a spray dryer.
  • the spray dryer may be equipped with a nozzle that atomizes the complex solution into droplets and a passing drying gas with a flowrate of 1 to 2000 Kg/h, and using temperatures ranging from 0 to 200°C, to dry the complex solution droplets into solid particles that are collected in a cyclone.
  • a powder with a high content of cyclodextrin / active pharmaceutical ingredient complex is obtained.
  • the present invention can be a continuous process or a non-continuous process.
  • setup A the solution A and solution B or solid API may be pre-mixed in a reactor, microfluidized and then recycled back to the same reactor producing the complex solution.
  • the said complex solution from this embodiment is dried, for example using spray drying or freeze drying.
  • the present invention discloses a method where solution A and solution B or solid API are microfluidized producing the complex solution.
  • the said complex solution is continuously fed to a continuous drier, for example a spray dryer.
  • the present invention has a high throughput due to the increased amount of solid complex obtained per process time. This is achieved due to the combination of three major effects: reduced dissolution times of components in solution A and solution B; reduced complexation times due to increased levels of mixing, milling and spontaneous temperature increasing the complex concentration in the complex solution; and finally by optionally continuously feeding said complex solution into the spray drying to obtain the solid complex. Examples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A complexation process between a cyclodextrin and active pharmaceutical ingredients is disclosed, and comprises a process for preparing a complex of at least one cyclodextrin and at least one active pharmaceutical ingredient comprising the steps of: a.Preparing a first solution (solution A) comprising at least one cyclodextrin and at least one solvent; b.Preparing a second solution (solution B) comprising at least one dissolved, partially dissolved or suspended API; c.Mixing said solution A and solution B by means of a microfludization system to produce a solution and/or suspension of at least one of said complex; d.Isolating said solution and/or suspension and/or optionally drying it; and e.Optionally collecting a powdered form of the complex. The described process has high throughput with higher yields of complexation in less time than prior art methods. The complexes obtained by the invention are characterized by having enhanced dissolution and/ or bioavailability of the active pharmaceutical ingredient in body fluids.

Description

Continuous complexation of active pharmaceutical ingredients
Field of Invention
The present invention relates generally to complexes of active pharmaceutical ingredients (API's), particularly to those based on one or more cyclodextrins, and to improved processes for the provision of such complexes.
Background of the invention
Several active pharmaceutical ingredients have low aqueous solubility which reduces their bioavailability and dissolution in body fluids. Presently, several techniques may be used to overcome this challenge, for example, amorphous solid dispersions, co- crystals, salt formation or cyclodextrin complexation.
Cyclodextrins are cyclic oligosaccharides compounds of 6, 7 or 8 repetitive units with an increasing hydrophobic cavity diameter. They can be designated respectively by alpha, beta or gamma depending on the number of repetitive units. Cyclodextrins can be, for example, prepared by enzymatic conversion of starch. Their external groups are hydrophilic and can be chemically modified, while their internal cavity can be hydrophobic which allows the inclusion of lipophilic molecules, for example, active pharmaceutical ingredients with reduced aqueous solubility.
The inclusion of active pharmaceutical ingredients with reduced aqueous solubility in the internal cyclodextrins cavity, or the formation of a cyclodextrin / active pharmaceutical ingredient complex leads to a high aqueous solubility compound and consequently higher bioavailability in body fluids.
A more detailed description of the advantages and applications of cyclodextrins to overcome the current solubility/bioavailability challenge can be found elsewhere in the literature, namely in the publication "Ciclodextrinas: como adjuvante tecnologico para melhorar a biodisponiblidade de farmacos", Lima Guedes et al., 2008. This review reports, in general terms, the importance of the use of cyclodextrins as pharmaceutical excipients through the years, it describes in detail cyclodextrins' main chemical derivatives, their mechanism of complexation, as well as their biopharmaceutical and toxicological implications. l The most common method used for the formation of cyclodextrin / active pharmaceutical ingredients complexes is characterized by a process where usually the cyclodextrin is dissolved in water, and then the active pharmaceutical ingredient in its solid form is added to the previous solution. By using, for example, agitation and / or heating, the active pharmaceutical ingredient partially dissolves in water and then forms the complex with the cyclodextrins, allowing the dissolution of additional active pharmaceutical ingredient in solution. To obtain the complex in the powder form, for example, a step of filtration or precipitation followed by drying is required.
The methods presented in the literature have several problems and drawbacks, namely, the high volumes of water usage, low yield due to low quantities of complexation formed, and high process times, requiring 24h to 172h to attain the maximum complex concentration, thus, exceeding the stability time of active pharmaceutical ingredients in solution. It is considered that these drawbacks make the industrial use of cyclodextrins less appealing, because of the low throughput. Moreover, the scale up of such processes is typically challenging and cumbersome. In US patent 6884885B2, 2005, the authors claim to solve the first problem by increasing the cyclodextrin concentration but no other indication is given that provides a solution for the remaining problems.
In US patent 5646131 and many other scientific papers, one or more hydrophilic polymers are used to increase the concentration of the complex formed. This makes the process more expensive, and free polymer will be present in the final powder which is considered to be disadvantageous.
In patent applications US2003/0012774A1 and WO2008052410A1 , homogenization processes are used and the introduction of energy by homogenization to increase the complexation of the co-enzyme Q10 in γ-cyclodextrin is mentioned. In both patent applications, co-enzyme Q10 is added in the solid state, requiring solubilization and subsequent complexation. The energy used in homogenization serves to solubilize the co-enzyme Q10 into the cyclodextrin solution, namely by decreasing the particle size.
In patent WO20151 14320 the authors claim a new scalable process to control the particle size and the particle size distribution of an API and/or excipient by: preparing a suspension in a mixture of solvents where said API and/or excipient is partially soluble in one of the said solvents; reducing the size of the particles present in said suspension using, for example, a homogenization process; ageing the suspension; and stopping the ageing by removing the mixture of solvents. Although the ageing step showed to be key to control the particle size distribution, for the formation of API inclusion complexes this step can be a disadvantage, as said complexes can rapidly dissociate. In general, the formation and dissociation rate of the cyclodextrin/API complexes is similar, and their half-life is only a few thousands of second in solution, meaning that said complexes are continuously being formed and dissociated (Lima Guedes et al., 2008).
In the publication "Physicochemical Characterizations of Ostholehydroxypropyl-β- cyclodextrin Inclusion Complexes with High-Pressure Homogenization Method", Liu et al., 2010 reported hydroxypropyl- cyclodextrin complexation with an active pharmaceutical ingredient, Osthole, using a microfluidization system. In this case a high pressure homogenizer followed by a filtration step and a drying step. This methodology is less efficient than the present invention since it comprises more process steps, mixture before the high pressure homogenizer, 3 cycles in high pressure homogenization and filtration before drying.
In the above-mentioned publications, the active pharmaceutical ingredient had low solubility in the cyclodextrin solution but still the authors tried to dissolve the active pharmaceutical ingredient in such conditions because of the presence of the cyclodextrin. This is considered to be time consuming and an energy waste. Also none of the referred publications provided a continuous process which is an important feature for the economy of the process.
In patents US6555139B2, US2002/0086061A1 , US2003/0091627A1 the authors use a microfluidization system to mill an active pharmaceutical ingredient using a non- solvent, water with a cyclodextrin dissolved. The objective of these publications is not to complex the active pharmaceutical ingredient but to reduce its particle size. In these cases the active pharmaceutical ingredient does not lose its solid form whereas in the present invention, the spontaneous particle size reduction is important for the complexation of the active pharmaceutical ingredient with the cyclodextrin. In these publications cyclodextrins are only used to confer the liquid certain properties and not to complex the API.
In the publication "Microfluidic Assembly of Cationic^-Cyclodextrin:Hyaluronic Acid- Adamantane Host:Guest pDNA Nanoparticles", Kulkarni et al, 2013, the authors use a microfluidic reactor to mix all components and to precipitate producing solid nanoparticles of complex using flash nanoprecipitation. The present invention considers that obtaining a solution of the complex is advantageous because it can be used in the liquid state for injectable systems and optionally can also be dried to obtain particles suitable for oral delivery. An objective of the present invention is to provide an improved process for complexation between cyclodextrins and active pharmaceutical ingredients without the disadvantages of the known processes and where it is possible to obtain the desired complexes in less time, with higher complex concentration, with lower solvent quantities, with the possibility of increasing the ratio of active pharmaceutical ingredient / cyclodextrin and where the energy input occurs in a spontaneous way. All these factors contribute to the overall economy of the process.
Unlike the current state-of-the-art, the present invention presents a continuous process of obtaining complexes consisting of a cyclodextrin and active pharmaceutical ingredients dissolved in suitable solvents using a microfluidization system with high levels of mixing and spontaneous heat generation that surprisingly allows complexes to be obtained in less time with high complexation efficiency and that can optionally be followed by spray drying to obtain a solid material.
General description of the invention
The present invention describes a continuous or not continuous process, in particular to obtain cyclodextrin/active pharmaceutical ingredient(s) complexes in reduced time.
According to the present invention, there is provided a process for preparing a complex of at least one cyclodextrin and at least one active pharmaceutical ingredient comprising the steps of: a. Preparing a first solution (solution A) comprising at least one cyclodextrin and at least one solvent;
b. Preparing a second solution (solution B) comprising at least one dissolved, partially dissolved or suspended API;
c. Mixing said solution A and solution B by means of a microfludization system to produce a solution and/or suspension of at least one of said complex;
d. Isolating said solution and/or suspension and/or optionally drying it; and
e. Optionally collecting a powdered form of the complex.
The present invention thus relates to the increased bioavailability of active pharmaceutical ingredients (API) preferably by the continuous formation of a complex of cyclodextrin and the active pharmaceutical ingredients using a microfluidization system. Alternatively the process can also be combined with spray drying to isolate the complex in powder form. More specifically, the present invention is related to a continuous or dis- continuous complexation process where a first solution of cyclodextrin is combined by microfluidization with a second solution, partial solution, or suspension of an active pharmaceutical ingredient (API)or the solid form of an active pharmaceutical ingredient (API), the first and second solutions (or partial solution, suspension, solid etc.) being either miscible or immiscible. The present invention also concerns the time reduction to obtain such a complex and the combination of a complexation step followed by a spray drying step which can make the present invention a continuous process with high complexation efficiency, where the obtained powder comprising the complex has high drug loading. The present invention thus represents a benefit in terms of reduction of process times.
The process may be a batch process or a continuous process.
Solution A preferably comprises at least one cyclodextrin comprising any substitution group and any cavity size, and/or a polymeric pharmaceutical excipient. The cyclodextrin may, for example, be one or more of a-cyclodextrin, β-cyclodextrin, γ- cyclodextrin, sulfobutylether-beta-cyclodextrin, hydropropyl - beta- cyclodextrin, methyl-beta-cyclodextrin and/or maltosyl - beta - cyclodextrin. A particularly preferred cyclodextrin is sulfobutylether - beta -cyclodextrin.
Solution A preferably comprises at least one of the following solvents: water, ethanol, methanol, isopropanol, dichloromethane acetone, methyl ethyl ketone, tetrahydrofuran, di-methyl sulfoxyde, di-methyl formaldehyde, or di-methyl acetamide.
The concentration of cyclodextrin in solution A preferably ranges from 1 to 50% (w/w).
If desired, a polymeric pharmaceutical excipient may be present in solution A, for example at a concentration of from 1 to 20%(w/w).
The pH of solution A typically ranges from 1 to 14, and a preferred pH is 6 to 8.
In the process of the invention, solution A is preferably prepared using a jacketed reactor with agitation, by adding at least one solvent to the reactor and at least one cyclodextrin to the same reactor and/or at least one polymeric pharmaceutical excipient to the same reactor, typically followed by pH adjustment. The order of the addition of the above components is not restricted and can be any way round.
Solution B preferably comprises at least one API dissolved in one or more solvents, partially dissolved in one or more solvents, or in suspension in one or more solvents. Preferably, the API or its pharmaceutically acceptable derivative has at least one functional group selected from: thiother, alcohol, thiol, aldehyde, ketone, thioketone, nitrate ester, phosphate ester, thiophosphate ester, ester, thioester, sulfate ester, carboxylic acid, phosphonic acid, sulfonic acid, amide, primary amine, secondary amine, ammonia, tertiary amine, imine, thiocyanate, cyanamide, oxime, nitrile, diazo, organohalide, nitro, S-heterocyclic ring, thiophene, N-heterocyclic ring, pyrrole, O- heteocyclic ring, furan, epoxide, peroxide, hydroxamic acid, imidazole, and pyridine.
Examples of preferred APIs include, but are not limited to, poorly soluble active compounds. Examples of poorly soluble compounds include, but are not limited to: antifungal agents like itraconazole or a related drug, such as fluoconazole, terconazole, ketoconazole and saperconazole; anti-infective drugs, such as griseofulvin and related compounds (e.g. griseoverdin); anti malaria drugs (e.g. Atovaquone); protein kinase inhibitors such as Afatinib, Axitinib, Bosutinib, Cetuximab, Crizotinib, Dasatinib, Erlotinib, Fostamatinib, Gefitinib, Ibrutinib, Imatinib, Zemurasenib, Lapatinib, Lenvatinib, Mubritinib or Nilotinib; immune system modulators (e.g. cyclosporine); cardiovascular drugs (e.g. digoxin and spironolactone); ibuprofen; sterols or steroids; drugs from the group comprising danazol, acyclovir, dapsone, indinavir, nifedipine, nitrofurantion, phentytoin, ritonavir, saquinavir, sulfamethoxazole, valproic acid, trimethoprin, acetazolamide, azathioprine, iopanoic acid, nalidixic acid, nevirapine, praziquantel, rifampicin, albendazole, amitrptyline, artemether, lumefantrine, chloropromazine, ciprofloxacin, clofazimine, efavirenz, iopinavir, folic acid, glibenclamide, haloperidol, ivermectin, mebendazole, niclosamide, pyrantel, pyrimethamine, retinol vitamin, sulfadiazine, sulfasalazine, triclabendazole, and cinnarizine.
Solution B preferably comprises at least one of the following solvents: water, ethanol, methanol, isopropanol, dichloromethane, acetone, methyl ethyl ketone, tetrahydrofuran, di-methyl sulfoxyde, di-methyl formaldehyde, or di-methyl acetamide.
In the invention, the one or more solvents in solution B can be the same or different from those solvents in Solution A, and if different said solvents can be miscible or immiscible with each other.
The concentration of API in solution B preferably ranges from 0.01 to 100% (w/w).
Solution B is typically prepared using a jacketed reactor with agitation, by adding at least one solvent to the reactor and at least one API. The order of addition of the components is not restricted and can be any way around. In one aspect, if desired, a solid API may be fed continuously, for example, by a positive displacement equipment, such as a screw-based feeding system or a pneumatic system.
In one preferred aspect, thee solid API is fed continuously, preferably using a hopper.
The microfludization preferably occurs in a system comprising at least one intensifier pump that can mix and displace a liquid mixed with another miscible or immiscible liquid, or alternatively a liquid mixed with a solid compound, into at least one micro- channel or micro-reactor. Two or more micro-channels or micro-reactors may be used if desired.
Preferably, the micro-channel or micro-reactor is a continuous flow reactor, suitably with a lateral dimension of equal to or below 1000 microns. The lateral dimension may be equal to or below 200 microns.
The hydrodynamic pressure of the process preferably ranges from 1 bar to 1500 bar, and may suitably range from 250 to 1000 bar.
In a preferred aspect of the invention, the feed flow ratio of solution A to solution B or solid API ranges from about 0.1 to about 10 kg/kg. The feed flow ratio of solution A and the amount of API added can range from 0.01 to 10 kg/kg.
The process temperature can be any suitable temperature, bearing in mind the components involved, and it suitably ranges from 0°C to 90°C.
Isolation of the complex from the complex solution and/or suspension may be by any means, but preferably comprises one or more drying techniques. One particularly suitable drying technique comprises spray drying.
In the process of the invention, isolation of the complex from the complex solution and/or suspension may be a batch process or continuous process.
In a preferred aspect, the complex is isolated in powdered form.
It will be appreciated that the complexes produced by the method of the invention may be used in medicine, for example as part of a pharmaceutical composition. The invention thus provides this use, or alternatively a process characterized in that the obtained complex in solution and/or suspension or powdered form is used for pharmaceutical purposes. In a preferred aspect, the invention provides a process for preparing a complex of at least one cyclodextrin and at least one active pharmaceutical ingredient comprising the steps of a) Preparation of Solution A with at least one cyclodextrin in one or more solvents;
b) Preparation of Solution B with at least one dissolved or suspended API. The solvent can be the same or different than Solution A, and if different can be miscible or immiscible. Alternatively the active pharmaceutical ingredient(s) are added in the solid state by means of a positive displacement equipment, preferably a hopper and a screw feed;
c) Continuous mixing of the solutions A and B or API in solid state using a microfluidization system that comprises an intensifier pump that draws and mix all components to the high pressure chamber creating cavitation and then displace the mixture into at least one micro-channel, forming a complex solution;
d) Optionally separate the complex from the solution continuously or not continuously by any method of separation such as crystallization, filtration or drying; preferably spray drying.
The present invention presents lower complexation times for the formation of the complex between the cyclodextrin and the at least one active pharmaceutical ingredient allowing to use the present invention for continuous manufacture of cyclodextrin complexes because of high levels of mixing and spontaneous heat generation that surprisingly create a high concentration of a desired complex. When combined, for example, with spray drying, it is possible to obtain a powder comprising the same complex.
The present invention also presents a method to obtain powder with higher concentration of the active pharmaceutical ingredient complexed with the cyclodextrin in less time than other known processes.
Preferentially, the present invention allows the continuous production of cyclodextrin / active pharmaceutical ingredient complex and its continuous isolation using spray drying.
The present invention comprises a microfluidization system. The microfluidization system typically consists of an intensifier pump that can mix and displace a liquid mixed with another miscible or immiscible liquid, or alternatively a liquid mixed with a solid compound, into a combination of micro-channels. A micro-channel is typically a continuous flow reactor with lateral dimensions less of 1000 μηι.
Preferentially, the present invention uses two different setups:
Setup A comprising: a) A reactor to prepare Solution A;
b) A positive displacement equipment such as an hopper and screw feed to displace a solid API into reactor a);
c) An intensifier pump;
d) A combination of at least one micro-channel.
Optionally a spray dryer and a cyclone to collect the powdered complex; Setup B a) A reactor to prepare Solution A;
b) A reactor to prepare Solution B;
c) An intensifier pump;
d) A combination of at least one micro-channel.
Optionally, a spray dryer and a cyclone to collect the powdered complex.
Brief Description of the Figures
Figure 1 shows a schematic flowchart of the present invention process.
Figure 2 shows a schematic drawing of setup A, where a) is a mixing reactor, b) is a positive displacement equipment, c) is an intensifier pump, d) a combination of at least one micro-channel, e) is a drying chamber and f) is a cyclone.
Figure 3 shows a schematic drawing of setup B, where a) is a mixing reactor, b) is a second mixing reactor, c) is an intensifier pump, d) a combination of at least one micro- channel, e) is a drying chamber and f) is a cyclone.
Figure 4 shows A representation of the concentration of complex formed vs time. Dotted line represents the example 1 method. The dashed line represents example 2 method. The solid line represents the example 3 method.
Detailed description of the invention The present invention presents a continuous or not continuous process for the complexation of a cyclodextrin and at least one active pharmaceutical ingredient optionally followed by a drying step. The drying step can, for example, be a spray drying process, but other suitable drying processes may be used.
More specifically, the present invention presents a continuous or not continuous (i.e. dis-continuous) process for the complexation of a cyclodextrin and at least one active pharmaceutical ingredient by mixing a solution A comprising at least one dissolved cyclodextrin and solution B comprising at least one dissolved or suspended API or alternatively the solid API fed by a positive displacement equipment, preferably a hopper equipped with a screw feed, forming stream B. Then using an intensifier pump, draw stream A and stream B and mix them into the pump high pressure chamber causing cavitation and then displace the mixed streams into at least one micro- channel, that creates high shear mixing, milling the API by cavitation, spontaneously produces heat due to friction and surprisingly produces a complex solution comprising high concentration of the complex. If combined with a spray drying step, the obtained complex solution is continuously or not continuously dried producing the complex of cyclodextrin and active pharmaceutical ingredient in the powder form.
Solution A comprises a solution with one or more solvents, that are preferably chosen from, water, ethanol, methanol, isopropanol, dichloromethane, acetone, Methyl Ethyl Ketone, TetraHydroFuran, Di-methyl Sulfoxyde, Di-methyl Formaldehyde, or Di-methyl Acetamide. The solution A also comprises one or more dissolved cyclodextrins or substituted cyclodextrins. Any cyclodextrin or substituted cyclodextrin may be used, and preferred compounds include, for example, a-cyclodextrin, β-cyclodextrin, γ- cyclodextrin, sulfobutylether - beta -cyclodextrin, hydropropyl - beta- cyclodextrin, methyl - beta - cyclodextrin and/or maltosyl - beta - cyclodextrin. Any suitable pharmaceutical excipient(s) can also be added to solution A with, for example, a concentration of from 1 %(w/w) to 20%(w/w). The concentration of the one or more cyclodextrins is preferably from 1 %(w/w) to 50%(w/w). Solution A has a pH value ranging from 1 to 14, preferably of from 6 to 8. Solution A can be prepared, for example, using a jacketed reactor with agitation, by adding solvent to the reactor and by adding cyclodextrin(s) to the same reactor, followed by the pH adjustment. The order of addition is nor restricted and can be done either way around.
Solution B comprises a solution or suspension using one or more solvents, which are preferably chosen from, water, ethanol, methanol, isopropanol, dichloromethane, acetone, Methyl Ethyl Ketone, TetraHydroFuran, Di-methyl Sulfoxyde, Di-methyl Formaldehyde, or Di-methyl Acetamide. Solution B also comprises at least one active pharmaceutical ingredient (API) which may be dissolved, partially dissolved or in suspension in the solvent.The API is preferably present at a concentration ranging from 0.01 % (w/w) to 100%(w/w). Alternatively, active pharmaceutical ingredients in their solid form can be used. Solution B may, for example, be prepared using a jacketed reactor with agitation where, the solvent or solvents and the at least one Active pharmaceutical ingredient are added to the reactor. The order of addition is not restricted and can be done either way around.
Alternatively, the at least one API in the solid form can be directly added to the mixture. Preferably, the API in solid form is added by a positive displacement equipment, preferably comprising a hopper or a screw feed or by pneumatic means.
Accordingly to the present invention, an optimum feed flow ratio between the Solution A and Solution B exists. Therefore, for example, a feed flow of Solution A to the microfluidization system can range from 0.1 to 10 times the Solution B feed flow to the microfluidization system. Ratios of 0.5 to 5, or 0.5 to 3, or 1 to 3 may also be used (as per above definition).
Alternatively, in the present invention, an optimum ratio between the feed flow rate of solution A and the amount of solid API added exists. Therefore, for example, a feed flow of Solution A to the microfluidics equipment can range from 0.01 to 10 times the amount of solid active pharmaceutical ingredient added. Ratios of 0.1 to 10, or 0.1 to 5, or 0.5 to 3, or 1 to 3 may also be used (as per above definition).
According to the present invention, the mixture of all compounds and complexation is performed in a microfluidization system. "Microfluidization" is a term understood by those skilled in this field. The term "microreaction" refers to a technology that involves physical and/or chemical reactions within microreactors, micromixers, microchannels or any other component comprised within the microfluidic field. The term "microfluidization" encompasses continuous fluid processing through these microchannels, involving high shear, cavitation and uniform mixing in the meso- and micromixing range. The microfluidization system comprises, for example, an intensifier pump that draws stream A and solution B or the at least one API in the solid form, continuously or not (i.e. dis-continuously), into the pump chamber and then pushes the mixture of streams through the at least one micro-channel, producing high pressure, spontaneous heat generation, friction, cavitation, high shear mixing at high Reynolds numbers, and milling if the API is in the solid state. We have found that, surprisingly, a high concentration of complex is obtained at the end of the pressurized pathway. The formation of the complex comprises the dissolution of the at least one API and consequently inclusion in the cavity of the at least one cyclodextrin. The number of micro-channels is not limited and can be at least one, but preferably rangesng from 1 to 10. The lateral dimensions of the micro-channels are lower than about 1000μηι, and are preferably equal to or lower than about 200 μηι. The pump creates pressures ranging from 1 to 1500 Bar (can we give some preferred sub-ranges?), and the mixed liquid temperature is spontaneously increased which represents an increase in efficiency as less energy is spent drying the mixed liquid. At the end of the process, a solution with a high concentration of the cyclodextrin / active pharmaceutical ingredient complex is obtained in much less time than reported previously.
The obtained solution can, if desired, be continuously or not continuously fed into a spray dryer. For example, the spray dryer may be equipped with a nozzle that atomizes the complex solution into droplets and a passing drying gas with a flowrate of 1 to 2000 Kg/h, and using temperatures ranging from 0 to 200°C, to dry the complex solution droplets into solid particles that are collected in a cyclone. At the end of the spray drying operation, a powder with a high content of cyclodextrin / active pharmaceutical ingredient complex is obtained.
The present invention can be a continuous process or a non- continuous process. For example, in a non-continuous embodiment, setup A, the solution A and solution B or solid API may be pre-mixed in a reactor, microfluidized and then recycled back to the same reactor producing the complex solution. Optionally, the said complex solution from this embodiment is dried, for example using spray drying or freeze drying.
In a continuous embodiment, for example setup B, the present invention discloses a method where solution A and solution B or solid API are microfluidized producing the complex solution. Optionally, the said complex solution is continuously fed to a continuous drier, for example a spray dryer.
The present invention has a high throughput due to the increased amount of solid complex obtained per process time. This is achieved due to the combination of three major effects: reduced dissolution times of components in solution A and solution B; reduced complexation times due to increased levels of mixing, milling and spontaneous temperature increasing the complex concentration in the complex solution; and finally by optionally continuously feeding said complex solution into the spray drying to obtain the solid complex. Examples
Example 1 :
60grams of water were added to a reactor with agitation. To this reactor 40grams of sulfobutylether-β- Cyclodextrin were added. The suspension was agitated continuously until a clear solution was formed. Then 1 gram of an active pharmaceutical ingredient (in this case Itraconazole was used as the model drug) was added to the said solution and a timer was immediately set. The formed suspension was agitated continuously at 25°C. Each day a 2g of said suspension was filtered and analyzed by HPLC to determine complex concentration. Results are shown in figure 4 (dotted line).
Example 2:
60grams of water were added to a reactor with agitation. To this reactor 40grams of sulfobutylether-β- Cyclodextrin were added. The suspension was agitated continuously until a clear solution was formed. Using setup A, 10 gram of an active pharmaceutical ingredient (itraconazole) was added to the said solution and a timer was immediately set. The formed suspension was fed to an intensifier pump at a pressure of 550bar at room temperature for 1 hour. At each 10min, a 2g sample from the suspension was filtered and analyzed by HPLC to determine complex concentration. After 1 h the solution was spray dried to produce a powdered material. Results are shown in figure 4 (dashed line).
Example 3:
60 grams of water were added to a reactor with agitation. To this reactor 400grams of sulfobutylether-β- Cyclodextrin were added. The suspension was agitated continuously until a clear solution was formed. Using setup A, 10 gram of an active pharmaceutical ingredient (itraconazole) was added to the said solution and a timer was immediately set. The formed suspension was fed to an intensifier pump at a pressure of 550bar and room temperature for 3 hours. At each 10min, a 2g sample from the suspension was filtered and analyzed by HPLC to determine complex concentration. After 3h the solution was spray dried to produce a powdered material. Results are shown in figure 4 (solid line).
Example 4:
60grams of water were added to a reactor with agitation. To this reactor 40grams of sulfobutylether-β- Cyclodextrin were added. In another reactor 90grams of dichloromethane were added. To this reactor 10grams of an active pharmaceutical ingredient (itraconazole) was added. Both solutions were agitated continuously until a clear solution was formed. Using setup B, both solutions were fed to an intensifier pump with a feed flow ratio of 10 kg/kg of solution A per solution B, and a pressure of 1000 bar at a temperature of 50°C was produced. The obtained solution was sampled to determine the complex concentration. The solution was then spray dried to form a powdered material.

Claims

1 A process for preparing a complex of at least one cyclodextrin and at least one active pharmaceutical ingredient comprising the steps of: f. Preparing a first solution (solution A) comprising at least one cyclodextrin and at least one solvent;
g. Preparing a second solution (solution B) comprising at least one dissolved, partially dissolved or suspended API;
h. Mixing said solution A and solution B by means of a microfludization system to produce a solution and/or suspension of at least one of said complex;
i. Isolating said solution and/or suspension and/or optionally drying it; and
j. Optionally collecting a powdered form of the complex.
2. A process according to claim 1 , wherein the process is a batch process or a continuous process.
3. A process according to claim 1 or 2, wherein solution A comprises at least one cyclodextrin comprising any substitution group and any cavity size, and/or a polymeric pharmaceutical excipient.
4. A process according to claim 3, wherein the the cyclodextrin is one or more of a-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, sulfobutylether - beta - cyclodextrin, hydropropyl - beta- cyclodextrin, methyl - beta - cyclodextrin and/or maltosyl - beta - cyclodextrin.
5. A process according to claim 4, wherein the cyclodextrin is sulfobutylether - beta -cyclodextrin.
6. A process according to any preceding claim, wherein solution A comprises at least one of the following solvents: water, ethanol, methanol, isopropanol, dichloromethane acetone, methyl ethyl ketone, tetrahydrofuran, di-methyl sulfoxyde, di-methyl formaldehyde, or di-methyl acetamide.
7. A process according to any preceding claim, wherein the concentration of cyclodextrin in solution A ranges from 1 to 50% (w/w).
8. A process according to any preceding claim, wherein a polymeric pharmaceutical excipient is present in solution A at a concentration of from 1 to 20%(w/w).
9. A process according to any preceding claim, wherein the pH of solution A ranges from 1 to 14.
10. A process according to claim 9, wherein the pH is 6 to 8.
1 1. A process according to any preceding claim, wherein solution A is prepared using a jacketed reactor with agitation, by adding at least one solvent to the reactor and at least one cyclodextrin to the same reactor and/or at least one polymeric pharmaceutical excipient to the same reactor, followed by pH adjustment.
12. A process according to claim 1 1 , wherein the order of addition of the components is not restricted and can be any way round.
13. A process according to any preceding claim, wherein solution B comprises at least one API dissolved in one or more solvents, partially dissolved in one or more solvents, or in suspension in one or more solvents.
14. A process according to claim 13, wherein the API or its pharmaceutically acceptable derivative has at least one functional group selected from: thiother, alcohol, thiol, aldehyde, ketone, thioketone, nitrate ester, phosphate ester, thiophosphate ester, ester, thioester, sulfate ester, carboxylic acid, phosphonic acid, sulfonic acid, amide, primary amine, secondary amine, ammonia, tertiary amine, imine, thiocyanate, cyanamide, oxime, nitrile, diazo, organohalide, nitro, S- heterocyclic ring, thiophene, N-heterocyclic ring, pyrrole, O-heteocyclic ring, furan, epoxide, peroxide, hydroxamic acid, imidazole, and pyridine.
15. A process according to any preceding claim, wherein solution B comprises at least one of the following solvents: water, ethanol, methanol, isopropanol, dichloromethane, acetone, methyl ethyl ketone, tetrahydrofuran, di-methyl sulfoxyde, di-methyl formaldehyde, or di-methyl acetamide.
16. A process according to any preceding claim, wherein the one or more solvents in solution B can be the same or different from those solvents in Solution A, and if different said solvents can be miscible or immiscible with each other.
17. A process according to any preceding claim, wherein the concentration of API in solution B ranges from 0.01 to 100% (w/w).
18. A process according to any one of claims 13 to 17, wherein solution B is prepared using a jacketed reactor with agitation, by adding at least one solvent to the reactor and at least one API.
19. A process according to claim 18, wherein the order of addition of the components is not restricted and can be any way around.
20. A process according to claim 18 or 19, wherein a solid API is be fed continuously by a positive displacement equipment, such as a screw-based feeding system or a pneumatic system.
21. A process according to claim 20, wherein the solid API is fed continuously preferably using a hopper.
22. A process according to any preceding claim, wherein the microfludization occurs in a system comprising at least one intensifier pump that can mix and displace a liquid mixed with another miscible or immiscible liquid, or alternatively a liquid mixed with a solid compound, into at least one micro-channel or micro-reactor.
23. A process according to claim 22, wherein the micro-channel or micro-reactor is a continuous flow reactor with a lateral dimension of equal to or below 1000 microns.
24. A process according to claim 23, wherein the lateral dimension is equal to or below 200 microns.
25. A process according to claim 23 or 24, wherein the hydrodynamic pressure of the process ranges from 1 bar to 1500 bar.
26. A process according to claim 25, wherein the hydrodynamic pressure of process ranges from 250 to 1000 bar.
27. A process according to any preceding claim, wherein the feed flow ratio of solution A to solution B ranges from 0.1 to 10 kg/kg.
28. A process according to any preceding claim, wherein the feed flow rate of solution A and the amount of solid API added can range from 0.01 to 10 kg/kg.
29. A process according to any preceding claim, wherein the process temperature ranges from 0°C to 90°C.
30. A process according to any preceding claim, wherein isolation of the complex from the complex solution and/or suspension comprises one or more drying techniques.
31. A process according to claim 30, wherein the drying technique comprises spray drying.
32. A process according to claims 30 or 31 , wherein isolation of the complex from the complex solution and/or suspension is a batch process or continuous process.
33. A process according to any one of claims 30 to 32, wherein the complex is isolated in powdered form.
34. A process according to any preceding claim, characterized in that the obtained complex in solution and/or suspension or powdered form is used for pharmaceutical purposes.
2/4
Figure imgf000021_0001
Figure 2
3/4
Figure imgf000022_0001
Figure 3
4/4
Figure imgf000023_0001
Figure 4
Figure imgf000023_0002
Figure 5
Figure 5 corresponds to a "zoom in" of the first 3 hours of Figure 4 for example 2 (dashed line) and 3 (solid line).
PCT/GB2017/050210 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients WO2017129988A1 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CN201780013795.1A CN108778338B (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
DK17707096.8T DK3400017T3 (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
ES17707096T ES2785625T3 (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
PL17707096T PL3400017T3 (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
CA3011820A CA3011820C (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
AU2017211520A AU2017211520B2 (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
JP2018539297A JP7038057B2 (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
US16/073,469 US11759529B2 (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
BR112018015481-4A BR112018015481B1 (en) 2016-01-28 2017-01-27 PROCESS FOR PREPARING A COMPLEX OF CYCLODEXTRIN AND ACTIVE PHARMACEUTICAL INGREDIENT
EP17707096.8A EP3400017B1 (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients
IL260766A IL260766B (en) 2016-01-28 2018-07-25 Continuous complexation of active pharmaceutical ingredients

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT10911716A PT109117B (en) 2016-01-28 2016-01-28 COMPLEXATION OF PHARMACEUTICAL ACTIVE INGREDIENTS
PT109117 2016-01-28

Publications (1)

Publication Number Publication Date
WO2017129988A1 true WO2017129988A1 (en) 2017-08-03

Family

ID=58159103

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2017/050210 WO2017129988A1 (en) 2016-01-28 2017-01-27 Continuous complexation of active pharmaceutical ingredients

Country Status (12)

Country Link
US (1) US11759529B2 (en)
EP (1) EP3400017B1 (en)
JP (1) JP7038057B2 (en)
CN (1) CN108778338B (en)
AU (1) AU2017211520B2 (en)
CA (1) CA3011820C (en)
DK (1) DK3400017T3 (en)
HU (1) HUE048601T2 (en)
IL (1) IL260766B (en)
PL (1) PL3400017T3 (en)
PT (1) PT109117B (en)
WO (1) WO2017129988A1 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019053247A1 (en) * 2017-09-18 2019-03-21 Ferring International Center S.A. Manufacturing of pharmaceutical compositions
US20190160005A1 (en) * 2017-11-24 2019-05-30 Biophore India Pharmaceuticals Pvt. Ltd. Method of Preparing Solid Dispersions of Active Pharmaceutical Ingredients
WO2019162688A1 (en) * 2018-02-22 2019-08-29 Hovione Scientia Limited A spray drying process with continuous preparation of spray solution
US11759529B2 (en) 2016-01-28 2023-09-19 Hovione Scientia Limited Continuous complexation of active pharmaceutical ingredients

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646131A (en) 1994-02-22 1997-07-08 The Arab Company For Drug Industries And Medical Applicances (Acdima) Method for solubilizing drugs using cyclodextrins and carboxylic acids
US20020086061A1 (en) 1999-10-26 2002-07-04 Sharma Vinay K. Preparation of micron-size felodipine particles by microfluidization
US20030012774A1 (en) 2000-01-27 2003-01-16 Jens Moldenhauer Method for producing a coenzyme q10/ y-cyclodextrin complex
US6555139B2 (en) 1999-06-28 2003-04-29 Wockhardt Europe Limited Preparation of micron-size pharmaceutical particles by microfluidization
US20030091627A1 (en) 2001-10-31 2003-05-15 Vinay Sharma Rate-controlled delivery of macrolides
US6884885B2 (en) 2000-12-21 2005-04-26 Cerestar Holding B.V. Production of cyclodextrin complexes
WO2008052410A1 (en) 2006-10-31 2008-05-08 Shenyang Wanjia Institute Of Biological Technology Research A supermolecule composition of water-soluble coenzyme q10 and preparation method thereof
WO2015114320A1 (en) 2014-01-28 2015-08-06 Hovione International Ltd Assisted particle size reduction process
WO2015154084A1 (en) * 2014-04-04 2015-10-08 Lam Therapeutics, Inc. An inhalable rapamycin formulation for treating age-related conditions

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9713149D0 (en) * 1997-06-21 1997-08-27 Pfizer Ltd Pharmaceutical formulations
WO2002056883A1 (en) 2001-01-18 2002-07-25 Wockhardt Limited Preparation of micron-size felodipine particles by microfluidization
CN1813751A (en) * 2005-11-24 2006-08-09 南京都药医药科技有限公司 Voriconazole injection and its preparing method
JP2008169379A (en) 2006-12-11 2008-07-24 Japan Science & Technology Agency Production method for inclusion compound
US7635773B2 (en) * 2008-04-28 2009-12-22 Cydex Pharmaceuticals, Inc. Sulfoalkyl ether cyclodextrin compositions
CN101507909A (en) * 2009-02-25 2009-08-19 中国科学院过程工程研究所 Molecular engram microspheres preparation method using micro-fluidic reactor
CN103690968A (en) * 2013-11-21 2014-04-02 石药集团中奇制药技术（石家庄）有限公司 Voriconazole composition and preparation method thereof
PT109117B (en) 2016-01-28 2019-02-01 Hovione Farm Sa COMPLEXATION OF PHARMACEUTICAL ACTIVE INGREDIENTS

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646131A (en) 1994-02-22 1997-07-08 The Arab Company For Drug Industries And Medical Applicances (Acdima) Method for solubilizing drugs using cyclodextrins and carboxylic acids
US6555139B2 (en) 1999-06-28 2003-04-29 Wockhardt Europe Limited Preparation of micron-size pharmaceutical particles by microfluidization
US20020086061A1 (en) 1999-10-26 2002-07-04 Sharma Vinay K. Preparation of micron-size felodipine particles by microfluidization
US20030012774A1 (en) 2000-01-27 2003-01-16 Jens Moldenhauer Method for producing a coenzyme q10/ y-cyclodextrin complex
US6884885B2 (en) 2000-12-21 2005-04-26 Cerestar Holding B.V. Production of cyclodextrin complexes
US20030091627A1 (en) 2001-10-31 2003-05-15 Vinay Sharma Rate-controlled delivery of macrolides
WO2008052410A1 (en) 2006-10-31 2008-05-08 Shenyang Wanjia Institute Of Biological Technology Research A supermolecule composition of water-soluble coenzyme q10 and preparation method thereof
WO2015114320A1 (en) 2014-01-28 2015-08-06 Hovione International Ltd Assisted particle size reduction process
WO2015154084A1 (en) * 2014-04-04 2015-10-08 Lam Therapeutics, Inc. An inhalable rapamycin formulation for treating age-related conditions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KULKARNI ET AL., MICROFLUIDIC ASSEMBLY OF CATIONIC-?-CYCLODEXTRIN:HYALURONIC ACID-ADAMANTANE HOST:GUEST PDNA NANOPARTICLES, 2013
LIMA GUEDES ET AL., CICLODEXTRINAS: COMO ADJUVANTE TECNOLOGICO PARA MELHORAR A BIODISPONIBLIDADE DE FARMACOS, 2008
LIU ET AL., PHYSICOCHEMICAL CHARACTERIZATIONS OF OSTHOIEHYDROXYPROPYI-?-CYCLODEXTRIN INCLUSION COMPLEXES WITH HIGH-PRESSURE HOMOGENIZATION METHOD, 2010

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11759529B2 (en) 2016-01-28 2023-09-19 Hovione Scientia Limited Continuous complexation of active pharmaceutical ingredients
WO2019053247A1 (en) * 2017-09-18 2019-03-21 Ferring International Center S.A. Manufacturing of pharmaceutical compositions
EA039921B1 (en) * 2017-09-18 2022-03-28 Ферринг Интернешнл Сентер С.А. Manufacturing of pharmaceutical compositions
US20190160005A1 (en) * 2017-11-24 2019-05-30 Biophore India Pharmaceuticals Pvt. Ltd. Method of Preparing Solid Dispersions of Active Pharmaceutical Ingredients
WO2019162688A1 (en) * 2018-02-22 2019-08-29 Hovione Scientia Limited A spray drying process with continuous preparation of spray solution
CN111954520A (en) * 2018-02-22 2020-11-17 好利安科技有限公司 Spray drying process for the continuous preparation of spray solutions
US11752105B2 (en) 2018-02-22 2023-09-12 Hovione Scientia Limited Spray drying process with continuous preparation of spray solution
IL276826B1 (en) * 2018-02-22 2024-10-01 Hovione Scientia Ltd A spray drying process with continuous preparation of spray solution

Also Published As

Publication number Publication date
AU2017211520A1 (en) 2018-08-16
US20190060486A1 (en) 2019-02-28
EP3400017B1 (en) 2020-02-19
PT109117B (en) 2019-02-01
HUE048601T2 (en) 2020-07-28
PT109117A (en) 2017-07-28
EP3400017A1 (en) 2018-11-14
US11759529B2 (en) 2023-09-19
DK3400017T3 (en) 2020-05-18
PL3400017T3 (en) 2020-07-13
JP7038057B2 (en) 2022-03-17
AU2017211520B2 (en) 2022-01-27
IL260766B (en) 2020-11-30
CA3011820A1 (en) 2017-08-03
BR112018015481A2 (en) 2018-12-18
CN108778338B (en) 2022-04-01
JP2019507127A (en) 2019-03-14
CN108778338A (en) 2018-11-09
CA3011820C (en) 2023-12-19

Similar Documents

Publication Publication Date Title
AU2017211520B2 (en) Continuous complexation of active pharmaceutical ingredients
JP7203078B2 (en) Continuous production method for particles
Kumar et al. Nanoparticle-based drugs and formulations: current status and emerging applications
Prosapio et al. Supercritical antisolvent coprecipitation mechanisms
Reverchon et al. Mechanisms controlling supercritical antisolvent precipitate morphology
Gunduz et al. Continuous generation of ethyl cellulose drug delivery nanocarriers from microbubbles
Dong et al. Direct comparison between millifluidic and bulk-mixing platform in the synthesis of amorphous drug-polysaccharide nanoparticle complex
Zhang et al. Microfluidic mixing and devices for preparing nanoparticulate drug delivery systems
Coty et al. Use of Spray Flash Evaporation (SFE) technology to improve dissolution of poorly soluble drugs: Case study on furosemide nanocrystals
Caggiano et al. Sequential Flash NanoPrecipitation for the scalable formulation of stable core-shell nanoparticles with core loadings up to 90%
Campardelli et al. Palmitoylethanolamide sub-micronization using fast precipitation followed by supercritical fluids extraction
US20110053927A1 (en) Nanoparticle formation via rapid precipitation
Khatri et al. Solubility enhancement techniques: an overview
Cui et al. Microfluidic fabrication of silybin nanodispersion with high dissolution rate and tunable sizes
Nandi et al. Development of long-acting injectable suspensions by continuous antisolvent crystallization: An integrated bottom-up process
ES2785625T3 (en) Continuous complexation of active pharmaceutical ingredients
BR112018015481B1 (en) PROCESS FOR PREPARING A COMPLEX OF CYCLODEXTRIN AND ACTIVE PHARMACEUTICAL INGREDIENT
Cruz‐Acuña et al. The roadmap to micro: Generation of micron‐sized polymeric particles using a commercial microfluidic system
Zhang et al. High-throughput preparation, scale up and solidification of andrographolide nanosuspension using hummer acoustic resonance technology
CN114502145A (en) Method for producing nanoparticulate active ingredients
Maddukuri et al. A comprehensive review on basic approaches to improve pharmaceutical solubility
Tao et al. N anoparticles, Acta Pharmaceutica Sinica B
Muhrer Gas antisolvent recrystallization of specialty chemicals
Odetade et al. Preparation of nanoliposomes and nanocrystals using microfluidic strategies [Poster]

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17707096

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 3011820

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 260766

Country of ref document: IL

ENP Entry into the national phase

Ref document number: 2018539297

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112018015481

Country of ref document: BR

WWE Wipo information: entry into national phase

Ref document number: 2017707096

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2017211520

Country of ref document: AU

Date of ref document: 20170127

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 2017707096

Country of ref document: EP

Effective date: 20180809

ENP Entry into the national phase

Ref document number: 112018015481

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20180727