Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/555—Heterocyclic compounds containing heavy metals, e.g. hemin, hematin, melarsoprol
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/16—Amides, e.g. hydroxamic acids
  • A61K31/165—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide
  • A61K31/167—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide having the nitrogen of a carboxamide group directly attached to the aromatic ring, e.g. lidocaine, paracetamol
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
  • A61K31/19—Carboxylic acids, e.g. valproic acid
  • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
  • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
  • A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/66—Phosphorus compounds
  • A61K31/661—Phosphorus acids or esters thereof not having P—C bonds, e.g. fosfosal, dichlorvos, malathion or mevinphos
  • A61K31/6615—Compounds having two or more esterified phosphorus acid groups, e.g. inositol triphosphate, phytic acid
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/08—Solutions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/10—Dispersions; Emulsions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
  • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P13/00—Drugs for disorders of the urinary system
  • A61P13/12—Drugs for disorders of the urinary system of the kidneys

Definitions

• the present invention is directed to methods and formulations for treatment of and/or protecting against acute liver failure and other hepatotoxic conditions, and associated renal injuries.
• the methods and formulations employ a first active agent which replenishes or decreases a loss of functional glutathione (GSH) in an individual, one example of which is N- acetylcysteine (NAC), and a second active agent comprising a manganese complex selected from a specified group, an example of which is a mixed metal complex of calcium and manganese, calmangafodipir, or a salt thereof.
• GSH functional glutathione
• NAC N- acetylcysteine
• a second active agent comprising a manganese complex selected from a specified group, an example of which is a mixed metal complex of calcium and manganese, calmangafodipir, or a salt thereof.
• acetaminophen also known in North America as acetaminophen and abbreviated as APAP, is known to induce acute liver failure (ALF) upon overdose. It is one of the most commonly used pharmaceuticals in the world and is often available without a doctor ' s prescription.
• Acetaminophen-induced ALF is characterized by massive hepatocyte cell death related to depletion of functional reduced glutathione (GSH).
• GSH is an important antioxidant in vivo and interacts with enzyme systems to prevent damage to important cellular components caused by reactive oxygen species.
• the ratio of reduced glutathione to oxidized glutathione (GS) within cells can be used to indicate the degree of cellular oxidative stress.
• NAPQI N-acetyl-p-benzoquinone
• Acetaminophen-induced liver cell damage is typically evidenced by release into the serum (i.e., serum activity) of liver intracellular enzymes such as aspartate aminotransferase (ASAT in Europe, or AST in the United States) or alanine aminotransferase ( ⁇ _ ⁇ / ⁇ _ ⁇ ).
• ASAT aspartate aminotransferase
• ⁇ _ ⁇ / ⁇ _ ⁇ alanine aminotransferase
• NAC N-acetylcysteine
• IV intravenous
• NAC treatment of ALF often involves complicated dosing schedules.
• One NAC intravenous dosing schedule involves a loading dose of 150 mg/kg infused over 1 hour in 200 ml of 5% dextrose solution, followed by less physiologically challenging maintenance doses of 50 mg/kg in 500 mL dextrose solution over 4 hours, and then 100 mg/kg in 1000 mL of dextrose solution over 16 hours.
• NAPQI mitochondrial dysfunction and cell death
• NAC treatment is complicated and so may carry some procedural risk. NAC treatment, though used widely, is not ideal as represented by the significant variation in dosages, dosing regimens, methods of administration, methods of monitoring treatment, and controversy over how long to maintain treatment. Additionally, higher doses and/or longer NAC regimens carry a possible risk of impeding hepatic recovery and presenting undesirable side effects, as discussed by Prescott et al, Eur. J. Clin. Pharmacol. , 37:501-506 (1989).
• Acetaminophen-induced ALF, and other ALF and hepatotoxic conditions are, in general, characterized by a failure of the body to handle disease-related or injury-related reactive oxygen species.
• Hepatotoxic conditions include hepatitis C, microbial infections, viral infections, and non-alcoholic steatohepatitis (NASH). They also include a wide variety of drug- induced liver injuries, including some related to modern medical treatments based on biopharmaceuticals such as monoclonal antibodies.
• acetaminophen metabolic, and biochemical such as nitrated tyrosine residues are known for ALF.
• Biomarkers are being used more and more in regard to diagnosis, monitoring, selection of treatment, and prognosis related to patient status as well as response to treatment. Given the acute but often varied onset nature of ALF, biomarkers hold promise to play a special role in regard to preventative treatments to reduce ALF conditions.
• Shi et al and Dear et al see also Harrill et al, Tox. Sciences, 110:235-243 (2009), and Ventethart et al, Clin. Pharmacol. Ther. (doi: 10.1002/cpt.541 , online November 30, 2016). The latter article discusses possible use of biomarkers to stratify patients by risk of liver injury prior to starting NAC.
• Acute kidney injury occurs in approximately 55% of all patients who present with acute liver failure (ALF) (Moore, Eur. J. Gastroenterol. Hepatol. , 1 1 (9):967-975 (1999)).
• ALF acute liver failure
• APAP acetaminophen
• renal injury may be related to complications which affect both the liver and kidneys, but, according to Moore, patients with renal injury will almost always recover if liver function can be recovered.
• a recent study of approximately 3000 cases of acetaminophen (APAP) overdose induced ALF concluded that the overall risk of such patients developing AKI was over two-fold higher than in controls (Chen et al, Medicine, 94(46):e2040 (2015)). While very few patients developed end-stage renal disease, Chen concluded that AKI is a possible adverse effect among patients with APAP intoxication, regardless of whether or not the patients presented with hepatic toxicity.
• ROS reactive oxygen species
• NAC N-acetylcysteine
• Manganese pyridoxyl ethylenediamine derivatives also sometimes referred to as manganese pyridoxyl ethyldiamine derivatives
• MnPLED-derivatives have been disclosed as having beneficial catalase, glutathione reductase and SOD mimetic activities, Laurent et al, Cancer Res, 65:948-956 (2005).
• MnPLED derivative mangafodipir, manganese N,N'-bis-(pyridoxal-5-phosphate)-ethylenediamine-N,N'-diacetic acid, also known as MnDPDP (CAS 146078-14-4), has been disclosed for protecting against and treating acetaminophen-induced ALF in mice, Bedda et al, J Hepatol, 39:765-772 (2003); Karlsson, J Hepatol, 40:872-873 (2004).
• MnDPDP is a manganese complex of fodipir, fodipir (Pubchem compound 60683, lUPAC Name: 2-[2-[carboxymethyl-[[3-hydroxy-2-methyl-5- (phosphonooxymethyl)pyridin-4-yl]methyl]amino]ethyl-[[3-hydroxy-2-methyl-5- (phosphonooxymethyl)pyridin-4-yl]methyl]amino]acetic acid).
• MnDPDP is dephosphorylated to an intermediate, MnDPMP, (manganese (II) N,N'-dipyridoxylethylenediamine-N,N'-diacetate-5- phosphate) and then to MnPLED (manganese (II) N,N'-dipyridoxylethylenediamine-N,N'- diacetate).
• MnDPMP manganese-MnPLED
• This dephosphorylation is thought to occur mainly by alkaline phosphatases rather than acid phosphatases in serum, according to in vitro metabolic rates and in vivo activities.
• the PledPharma AB WO 2011/004325 discloses that a mixture of a manganese complex compound such as mangafodipir and a non-manganese PLED-derivative compound such as N,N'-bis-(pyridoxal-5-phosphate)-ethylenediamine-N,N'-diacetic acid, also known as DPDP, can provide therapeutic advantages over mangafodipir alone in treating a variety of reactive oxygen species (ROS)-related disease conditions, including acetaminophen-induced ALF. Surprisingly, the mixture reduces the possibility of cerebral and other complications related to release of manganese from mangafodipir in the body.
• ROS reactive oxygen species
• the PledPharma AB WO 2013/102806 discloses the use of calcium-manganese mixed metal PLED derivatives, and, specifically, calcium-manganese mixed metal complexes of N,N'-bis-(pyridoxal-5-phosphate)- ethylenediamine-N,N'-diacetic acid (DPDP).
• a specific calcium-manganese mixed metal complex of DPDP is calmangafodipir (CAS 1401243-67-1), also known as Ca 4 MnDPDP 5 , abbreviated herein as "CaM" .
• CaM provides therapeutic advantages over mangafodipir alone in treating a variety of ROS-related disease conditions, including acetaminophen-induced ALF.
• the mixed metal complex calmangafodipir reduces the possibility of cerebral and other complications related to release of manganese from mangafodipir in the body and also provide important improvements in production, formulation, and therapeutic administration. See Karlsson et al., Drug Disc. Today 20:41 1-421 (2015).
• GSH is involved as a reagent in complex biochemical systems which include enzymes such as superoxide dismutase, catalase and glutathione reductase, which act to prevent oxidative stress (OS) caused by overproduction of reactive oxygen species (ROS) such as OH “ , H 2 0 2 and 0 2 " , as well as reactive nitrogen species (RNS) such as peroxynitrite (ONOO " ), Valko, Int. J. Biochemistry & Cell Biology, 39:44-84 (2007).
• ROS reactive oxygen species
• RNS reactive nitrogen species
• the latter can covalently modify biological molecules including proteins via nitration or nitrosylation.
• Superoxide dismutase (SOD) enzymes are known to readily react with superoxide radicals and convert them to hydrogen peroxide which the enzyme catalase can convert to water and oxygen.
• SOD superoxide dismutase
• Peroxynitrite plays a nefarious role in OS in general and particularly in ALF conditions such as
• acetaminophen overdose as it is able to react with a tyrosine residue in the active site of SOD, with the resulting nitration of the tyrosine residue compromising SOD enzymatic activity. This results in an increase of superoxide, as well as the formation of peroxynitrite, Agarwal, J.
• SOD enzyme catalytic activities are related to metal (typically Ni or Mn)-chelated cofactors and some compounds with metal chelating groups such as porphyrins are sometimes referred to "peroxynitrite decomposition agents or catalysts" (Pieper, J. Pharmacol. & Exp. Therapeutics, 314:53-60 (2005).
• NAC is not the only reducing compound that is used or under investigation for replenishing intracellular glutathione levels under disease circumstances related to oxidative stress.
• Other compounds which have been studied include methionine and methionine analogues such as DL-methionine, D-methionine, N-acetyl-methionine (Garlick, The Journal of Nutrition, 136: 1722S-1725S (2006)), N-acetyl-cysteine-amide (Wu et al, Biomed.
• MnPLED compounds are known to have SOD catalytic mimetic properties and, in some cases, have also been found to have catalase and glutathione reductase mimetic activities (Karlsson, 2015). None of these enzymatic activities are associated with NAC or related chemical antioxidant compounds.
• Additional improvements in treating and protecting against acetaminophen-induced ALF, other ALF conditions, and hepatotoxic conditions including, but not limited to, those associated with administration of therapeutic agents which cause ALF, hepatitis C, microbial infections, viral infections, including but not limited to HIV infection, non-alcoholic steatohepatitis (NASH), and certain inherited disorders such as Wilson ' s Disease, and alpha-1 -antitrypsin deficiency, are desired.
• the present invention is directed to methods and formulations for treating and/or protecting against ALF and other hepatotoxic conditions.
• the present invention is directed to methods and formulations for treating and/or protecting against AKI and related complications concomitant with ALF and other hepatotoxic conditions.
• protecting against includes, in one embodiment, preventing, the indicated condition, and/or reducing the extent of development of the indicated condition, particularly in an individual at risk of development of the indicated condition.
• the invention is directed to a method of treating and/or protecting against acute liver failure induced by an acetaminophen overdose in an individual.
• the method comprises (a) administering to the individual an effective amount of a first active agent which replenishes, or decreases a loss of, functional glutathione in the individual, and (b) administering an effective amount of a second active agent comprising a manganese complex and selected from the group consisting of (i) a calcium manganese mixed metal complex of N,N'-bis-(pyridoxal-5-phosphate)-ethylenediamine-N,N'-diacetic acid (DPDP) having a molar ratio of calcium to manganese in a range of from 1 to 10, or a pharmaceutically acceptable salt thereof, (ii) a mixture of manganese DPDP (MnDPDP), or a pharmaceutically acceptable salt thereof, and a non-manganese-containing DPDP compound, or (iii) a mixture of manganese DPDP
• the invention is directed to a method of treating and/or protecting against acute liver failure in an individual.
• the method comprises (a) administering to the individual an effective amount of a first active agent which replenishes, or decreases a loss of, functional glutathione in the individual, and (b) administering an effective amount of a second active agent comprising a manganese complex and selected from the group consisting of (i) a calcium manganese mixed metal complex of DPDP having a molar ratio of calcium to manganese in a range of from 1 to 10, or a pharmaceutically acceptable salt thereof, (ii) a mixture of MnDPDP, or a pharmaceutically acceptable salt thereof, and a non-manganese- containing DPDP compound, or (iii) a mixture of MnPLED, or a pharmaceutically acceptable salt thereof, and a non-manganese-containing PLED compound, to the individual.
• a first active agent which replenishes, or decreases a loss of, functional glutathione in
• the invention is directed to a method of treating and/or protecting against hepatotoxicity in an individual.
• the method comprises (a) administering to the individual an effective amount of a first active agent which replenishes, or decreases a loss of, functional glutathione in the individual, and (b) administering an effective amount of a second active agent comprising a manganese complex selected from the group consisting of (i) a calcium manganese mixed metal complex of DPDP having a molar ratio of calcium to manganese in a range of from 1 to 10, or a pharmaceutically acceptable salt thereof, (ii) a mixture of MnDPDP, or a pharmaceutically acceptable salt thereof, and a non-manganese- containing DPDP compound, or (iii) a mixture of MnPLED, or a pharmaceutically acceptable salt thereof, and a non-manganese-containing PLED compound, to the individual.
• a first active agent which replenishes, or decreases a loss of, functional glutathione
• the invention is directed to a method of reducing acute kidney injury associated with acute liver failure or other hepatotoxicity in an individual.
• the method comprises (a) administering an effective amount of a second active agent comprising a manganese complex selected from the group consisting of (i) a calcium manganese mixed metal complex of DPDP having a molar ratio of calcium to manganese in a range of from 1 to 10, or a pharmaceutically acceptable salt thereof, (ii) a mixture of MnDPDP, or a pharmaceutically acceptable salt thereof, and a non-manganese-containing DPDP compound, or (iii) a mixture of MnPLED, or a pharmaceutically acceptable salt thereof, and a non- manganese-containing PLED compound, to the individual, and optionally (b) administering to the individual an effective amount of a first active agent which replenishes, or decreases a loss of, functional glutathione in the individual.
• a second active agent comprising a manganese complex selected from the group consist
• the invention is directed to a therapeutic method of administering a high dosage of acetaminophen to an individual.
• the method comprises administering a therapeutic high dosage of acetaminophen to the individual, and administering an effective amount of a second active agent comprising a manganese complex selected from the group consisting of (i) a calcium manganese mixed metal complex of DPDP having a molar ratio of calcium to manganese in a range of from 1 to 10, or a pharmaceutically acceptable salt thereof, (ii) a mixture of MnDPDP, or a pharmaceutically acceptable salt thereof, and a non- manganese-containing DPDP compound, or (iii) a mixture of MnPLED, or a pharmaceutically acceptable salt thereof, and a non-manganese-containing PLED compound, to the individual.
• the method may also include administering to the individual an effective amount of a first active agent which replenishes, or decreases a loss of, functional glutathione
• the invention is directed to a formulation comprising a first active agent which replenishes, or decreases a loss of, functional glutathione in an individual, and a second active agent comprising a manganese complex selected from the group consisting of (i) a calcium manganese mixed metal complex of DPDP having a molar ratio of calcium to manganese in a range of from 1 to 10, or a pharmaceutically acceptable salt thereof, (ii) a mixture of MnDPDP, or a pharmaceutically acceptable salt thereof, and a non-manganese- containing DPDP compound, or (iii) a mixture of MnPLED, or a pharmaceutically acceptable salt thereof, and a non-manganese-containing PLED compound.
• the invention is directed to a kit containing at least one such formulation. [0026] The methods, formulations and kits provide improvements in treating and/or protecting against ALF and other hepatotoxic conditions.
• Fig. 1 shows the relative reduction of N-acetylcysteine (NAC) concentration versus storage time of NAC:CaM Test Mixture 1 at room temperature (RT, 22 °C) and +4 °C, as described in Example 2.
• NAC N-acetylcysteine
• Fig. 2 shows the relative reduction in N-acetylcysteine (NAC) concentration versus storage time of NAC:CaM Test Mixture 3 at room temperature (RT, 22 °C) and +4 °C, as described in Example 2.
• NAC N-acetylcysteine
• Fig. 3 shows the relative reduction in calmangafodipir (CaM) concentration versus storage time of NAC:CaM Test Mixture 1 at room temperature (RT, 22 °C) and +4 °C, as described in Example 2.
• Fig. 4 shows the relative reduction in calmangafodipir (CaM) concentration versus storage time of NAC:CaM Test Mixture 3 at room temperature (RT, 22 °C) and +4 °C, as described in Example 2.
• Fig. 5 shows the formation of ⁇ , ⁇ ' -diacetylcystine (diNAC) versus storage time of NAC:CaM Test Mixtures 1 and 3 at room temperature (RT, 22 °C) and +4 °C, as described in Example 2.
• diNAC diacetylcystine
• ALAT alanine aminotransferase
• CaM calmangafodipir
• the methods, formulations and kits of the invention employ a first active agent and a second active agent.
• the first active agent and the second active agent may be administered together or separately, as discussed in further detail below.
• the first active agent replenishes, or decreases a loss of, functional glutathione in the individual, i.e. , restores or assists in restoring functional glutathione to a normal range, such as that experienced in a healthy individual.
• Functional glutathione is glutathione (in any form) which functions in vivo, preventing damage to cellular components, such as by covalently reacting with NAPQI to render it less toxic.
• the first active agent may be administered in an effective amount, i.e., an amount effective to at least partially replenish, or decrease a loss of, functional glutathione in the individual which has occurred owing to acetaminophen overdose or other hepatotoxicity- inducing event or condition.
• the first active agent comprises N- acetylcysteine (NAC), cysteine, homocysteine, glycyrrhizin, GSH, methionine, a methionine analogue (DL-methionine, D-methionine, and/or N-acetyl-methionine), N- acetyl-cysteine-amide, or a combination thereof.
• the first active agent comprises NAC.
• a specific effective dosage of the first active agent for a particular patient may be determined by one of ordinary skill in the art in view of the present disclosure.
• the first active agent comprises NAC, cysteine, homocysteine, glycyrrhizin, GSH, methionine, or a combination thereof
• the first active agent, or, specifically, NAC may be administered in a total dosage amount of 100-500 mg/kg body weight, in accordance with current conventional treatment therapies, typically administered with an initial/loading dosing regimen of 150 mg/kg, followed by maintenance dosages of 50 to 100 mg/kg.
• the first active agent comprises NAC, cysteine, homocysteine, glycyrrhizin, GSH, methionine, or a combination thereof
• the first active agent, or, specifically, NAC may be employed in an amount less than that conventionally employed.
• the first active agent, or, specifically, NAC may be administered in a total dosage of from about 10 to 200 mg/kg body weight, or, more specifically, from about 10 to 150 mg/kg body weight, from about 10 to 100 mg/kg body weight, or from about 10 to 50 mg/kg body weight.
• the total dosage may be administered in a single administration or in an initial administration followed by one or more additional administrations. Therefore, these embodiments are advantageous in employing a lower level of the first active agent as compared with various conventional NAC treatment methods.
• the second active agent comprises a manganese complex which exhibits SOD mimetic activity, and, optionally, catalase, glutathione reductase and/or other mimetic activity.
• the manganese complex is selected from the group consisting of (i) a calcium manganese mixed metal complex of N,N'-bis-(pyridoxal-5-phosphate)-ethylenediamine-N,N'-diacetic acid (DPDP) having a molar ratio of calcium to manganese in a range of from 1 to 10, or a pharmaceutically acceptable salt thereof, (ii) a mixture of manganese DPDP (MnDPDP), or a pharmaceutically acceptable salt thereof, and a non-manganese-containing DPDP compound, or (iii) a mixture of manganese pyridoxyl ethylenediamine (MnPLED), or a pharmaceutically acceptable salt thereof, and a non-manganese-containing pyridoxyl ethylenediamine (
• calmangafodipir refers to a calcium- manganese mixed metal complex of MnDPDP, containing an approximate calcium to manganese molar ratio of 4:1 , also known as Ca 4 MnDPDP 5 , abbreviated herein as "CaM”.
• CaM calcium to manganese molar ratio of 4:1
• Calmangafodipir is disclosed in WO 2013/102806 A1 , which is incorporated herein in its entirety.
• the second active agent comprises calmangafodipir.
• a non-manganese-containing DPDP compound refers to N,N'-bis-(pyridoxal-5-phosphate)-ethylenediamine-N,N'-diacetic acid (DPDP), a metal complex of DPDP which does not contain manganese, i.e., a calcium complex, or a pharmaceutically acceptable salt of DPDP or of such a metal complex.
• DPDP N,N'-bis-(pyridoxal-5-phosphate)-ethylenediamine-N,N'-diacetic acid
• MnDPDPDP manganese
• the mixture of MnDPDP, or a pharmaceutically acceptable salt thereof, and a non- manganese-containing DPDP compound comprises MnDPDP and CaDPDP, or salts thereof.
• a non-manganese-containing PLED compound refers to pyridoxyl ethylenediamine (PLED), a metal complex of PLED which does not contain manganese, i.e., a calcium complex, or a pharmaceutically acceptable salt of PLED or of such a metal complex.
• the molar ratio of the non-manganese- containing PLED compound to MnPLED, or pharmaceutically acceptable salt thereof is in a range of from 1 to 10.
• the mixture of MnPLED, or a pharmaceutically acceptable salt thereof, and a non-manganese-containing PLED compound comprises MnPLED and CaPLED, or salts thereof.
• Suitable pharmaceutically acceptable salts of the mentioned DPDP- and PLED- containing compounds both those containing manganese, and those not containing
• manganese include, but are not limited to, sodium salts, with one or more hydrogen ions replaced by sodium.
• CaM and MnDPDP, and salts thereof are pro-drugs in the sense that they metabolize in vivo into related PLED derivatives such as MnPLED.
• the second active agent is employed in an effective amount, i.e. , an amount effective to reduce the oxidative stress in the individual which occurred owing to acetaminophen overdose, or other hepatotoxicity-inducing event or condition, through SOD mimetic activity, and/or other activity, for example, catalase, glutathione reductase, and/or other activity.
• the second active agent may improve the effectiveness of the first active agent.
• a specific effective dosage of the second active agent for a particular patient may be determined by one of ordinary skill in the art in view of the present disclosure.
• the second active agent is administered in a dosage of from about 0.01 to 50 mg/kg body weight, from about 0.1 to 25 mg/kg body weight, or from about 0.1 to 10 mg/kg body weight.
• calmangafodipir is administered in a dosage of from about 0.01 to 50 mg/kg body weight, from about 0.1 to 25 mg/kg body weight, or from about 0.1 to 10 mg/kg body weight.
• a mixture of MnDPDP, or a salt thereof, and a non-manganese containing DPDP compound is administered in a dosage of from about 0.01 to 50 mg/kg body weight, from about 0.1 to 25 mg/kg body weight, or from about 0.1 to 10 mg/kg body weight.
• a mixture of MnPLED, or a salt thereof, and a non-manganese containing PLED compound is administered in a dosage of from about 0.01 to 50 mg/kg body weight, from about 0.1 to 25 mg/kg body weight, or from about 0.1 to 10 mg/kg body weight.
• liver intracellular enzymes such as aspartate
• an effective amount includes an amount which reduces serum ALAT and/or ASAT.
• a method of treating acute liver failure induced by an acetaminophen overdose in an individual comprises (a) administering to the individual an effective amount of a first active agent which replenishes, or decreases a loss of, functional glutathione in the individual, and (b) administering an effective amount of a second active agent selected from the group consisting of (i) a calcium manganese mixed metal complex of DPDP having a molar ratio of calcium to manganese in a range of from 1 to 10, or a pharmaceutically acceptable salt thereof, (ii) a mixture of MnDPDP, or a pharmaceutically acceptable salt thereof, and a non-manganese-containing DPDP compound, or (iii) a mixture of MnPLED , or a pharmaceutically acceptable salt thereof, and a non-manganese-containing PLED compound, to the individual.
• a second active agent selected from the group consisting of (i) a calcium manganese mixed metal complex of DPDP having a molar ratio
• the inventive method is particularly advantageous for use in situations in which an individual may not currently have received immediate treatment for an acetaminophen overdose and/or in which conventional NAC treatment is either not yet started, for example, if the individual is being monitored for impending ALF or the overdose-induced ALF has progressed to a point at which conventional NAC treatment alone may not be as effective as desired.
• the individual prior to administration of the first active agent or the second active agent, the individual will have been determined to be in need of a treatment to reduce the probability of oxidative stress leading to hepatocyte cell death.
• the method may therefore comprise determining a level of at least one biomarker indicative of acute liver failure induced by an acetaminophen overdose.
• biomarkers may include, but are limited to one or more of, paracetamol-protein adducts (for example, paracetamol-cysteine), microRNA- 122 (miR-122), keratin-18 (K-18), high-mobility group box-1 (HMGB1), glutamate
• first active agent and the second active agent may provide longer therapeutic effect as compared with conventional NAC treatment, as NAC therapeutic benefit is expected to stop soon after NAC administration is discontinued. This has been shown to not be the case for the second active agent comprising a SOD enzymatic mimetic such as CaM or MnDPDP, which can provide extended therapeutic treatment of oxidative stress in vivo after administration.
• Employing the first and the second active agent may offer other therapeutic effects such as reduced chance of underdosing and also synergistic efficacy enhancements which are not simply additive in nature. The latter is discussed in more detail below.
• the weight ratio of the first active agent to the second active agent may vary as desired.
• the weight ratio of the first active agent to the second active agent is in a range of from 300: 1 , 250: 1 , 200: 1 , or 150: 1 to 1 : 1 , from 100: 1 to 1 : 1 , from 50: 1 to 1 :1 , from 20: 1 to 1 : 1 , or from 10: 1 to 1 :1.
• a typical NAC to calmangafodipir (CaM) ratio (w/w) of 30 is used (e.g., NAC 150 mg/kg and CaM 5 mg/kg).
• a typical NAC to CaM ratio (w/w) of 6 is used (e.g., NAC 30 mg/kg and CaM 5 mg/kg).
• the first active agent is a "stoichiometric" compound which replenishes a depleted functional glutathione level and is expected to be consumed or otherwise altered as it functions in vivo, such as during conjugation to NAPQI.
• the second active agent acts catalytically through its superoxide dismutase (SOD) or related enzyme mimetic activity.
• SOD superoxide dismutase
• the first active agent and the second active agent may not only exhibit different mechanisms for reducing reactive oxygen species (ROS), and affect different ROS targets, but they may act at different cellular sites.
• ROS reactive oxygen species
• NAC is quite hydrophilic, and though actively transported into cells, it may be expected to be less able to passively partition into and through lipid membranes than the more lipophilic MnPLED metabolic products resulting from Calmangafodipir, MnDPDP or MnPLED
• antioxidants function via different mechanisms at different cellular sites. Use of combinations of antioxidants to treat various diseases has therefore been suggested. However, a combination of antioxidants cannot be assumed to function in vivo as desired due to several practical reasons.
• a mixture of two antioxidants may, for example, form an insoluble complex, chemically react to form a third non-functional compound, chemically alter (i.e. , reduce or oxidize) each other in a manner to render one or both of them non-functional, and/or affect the patient in a manner to reduce the efficacy and/or enhance the toxicity of one or both antioxidants.
• the present inventors have discovered that a mixture of the first active agent and the second active agent, particularly, NAC and MnPLED or a MnPLED derivative compound such as calmangafodipir or MnDPDP, remains stable, as is demonstrated in Examples 1 and 2, and is efficacious, as demonstrated in Example 4.
• the first active agent and the second active agent may be substantially simultaneously administered to the individual, in one or separate formulations, or may be administered sequentially, for example with less than 1 hour, or 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10 or more hours between administrations.
• the second active agent may be administered to the individual subsequent to administration of the first active agent or, alternatively, prior to administration of the first active agent.
• the active agents may be administered in one or more formulations in solution form or in a freeze-dried formulation, or in other conventional pharmaceutically acceptable forms, optionally including one or more conventional pharmaceutical excipients or carriers. Calmangafodipir and mixtures of MnDPDP and other DPDP compounds are advantageously deliverable with water-soluble carriers.
• formulations of these mixtures may advantageously include one or more excipient additives, including but not limited to, surfactants, micelles, or liposomes, to present the active components of the mixture in a less lipophilic (less hydrophobic) state and thus more suitable for delivery intravenously in a water-soluble formulation.
• excipient additives including but not limited to, surfactants, micelles, or liposomes
• Various dosing regimens may also be employed wherein administration of the first active agent and the second active agent are alternated, or substantially simultaneous administration is followed by one or more individual administrations. For example, an additional dosage of the second active agent may be administered subsequent to a substantially simultaneous administration of the first active agent and the second active agent.
• At least one of the first active agent and the second active agent are administered to the individual at a time 8 hours or more subsequent to the acetaminophen overdose.
• the second active agent is administered to the individual at a time 8 hours or more subsequent to the acetaminophen overdose. It is recognized however that the degree or time of an overdose occurrence is not always established at the time an individual is presented for treatment, so a physician may elect to begin treatment prior to or while conducting one or more laboratory tests to determine the degree or timing of an overdose.
• first agent may be administered to the individual, with and/or alternating with administration of the second agent.
• a first administration of the first agent is followed by an administration of the second agent, which, in turn is followed by a second administration of the first agent.
• the administration of the second agent may be closer in time to either the first administration or the second administration of the first agent, or may be spaced substantially equally in time therebetween.
• a formulation according to the invention comprises both the first active agent and the second active agent.
• the weight ratio of the first active agent and the second active agent may be varied as desired.
• the weight ratio of the first active agent and the second active agent is in a range of from 300: 1 , 250: 1 , 200:1 , or 150: 1 to 1 : 1 , from 100: 1 to 1 :1 , from 50: 1 to 1 : 1 , or from 20: 1 to 1 : 1.
• the amount of the first active agent, or, specifically, NAC, in the formulation may be sufficient to provide a dosage of from about 10 to 300 mg/kg body weight.
• the amount of the second active agent, or, more specifically, calmangafodipir, in the formulation may be sufficient to provide a dosage of from about 0.01 to 50 mg/kg body weight, from about 0.1 to 25 mg/kg body weight, or from about 0.1 to 10 mg/kg body weight.
• the formulation may be in solution form, a dispersion or emulsion form, or a solid form, including a tablet or powder, and may comprise a freeze-dried formulation.
• the formulation comprises the first active agent, or, specifically, NAC, in an amount of from about 10 to 200 mg/kg body weight, or, more specifically, from about 10 to 150 mg/kg body weight, from about 10 to 100 mg/kg body weight, or from about 10 to 50 mg/kg body weight. Therefore, these embodiments are advantageous in employing a lower level of the first active agent as compared with various conventional NAC treatment methods, thereby providing treatment with a simple and more benign (less adverse side effects) dosing regimen.
• first active agent and the second active agent may provide longer therapeutic effect, as compared with conventional NAC treatment, as NAC therapeutic benefit is expected to stop soon after NAC administration is discontinued.
• second active agent SOD mimetics such as CaM and MnDPDP, which provide extended therapeutic treatment of oxidative stress in vivo after adminsitration. Therefore, the combination formulations should provide improved treatments.
• the invention is directed to a kit for treating acute liver failure.
• the kit comprises at least one formulation as described, and at least one separate, i.e., separately packaged, formulation comprising the second active agent.
• the kit may comprise at least one formulation as described and one or more additional formulations of the first active agent.
• Further embodiments may include at least one formulation as described and one or more formulations of both the first active agent and the second active agent in relative amounts which vary from those in the at least one formulation.
• the kit may also include instructions for administration of the formulation(s) and/or instructions for selection of one or more formulations for administration to a patient from several formulations in the kit.
• the invention is directed to a method of treating acute liver failure comprising administering the first active agent to the individual and administering the second active agent to the individual.
• the invention is directed to a method of treating hepatotoxicity, comprising administering the first active agent to the individual and administering the second active agent to the individual.
• the ALF or hepatotoxicity may be the result of acetaminophen overdose or other hepatotoxic conditions, including, but not limited to, those associated with administration of other therapeutic agents which cause ALF, hepatitis C, microbial infections, viral infections, including but not limited to HIV infection, and/or NASH. Additionally, the dosing amounts, regimen variations and formulations discussed above may be equally applied in these methods.
• the invention is directed to a method of administering a therapeutic high dosage of acetaminophen to an individual, comprising administering a therapeutic high dosage of acetaminophen to the individual, and administering the second active agent to the individual in an amount effective to reduce or protect against liver damage by the high dosage of acetaminophen.
• a high dosage of acetaminophen is desirable, for example, for acute pain or acute fever, but would otherwise be avoided owing to the concomitant toxic effect.
• the second active agent dosing amounts discussed above may be equally applied in this method.
• the methods may also include administration of the first active agent as described herein, and the dosing amounts, regimen variations and formulations discussed above for the first active agent may be equally applied in such methods.
• Example 1 The following Examples demonstrate various aspects of the invention.
• This Example describes short and long term stability studies involving mixtures of Calmangafodipir and NAC. Each mixture was formed by adding CaM (in the form of a trisodium salt powder) and NAC to deionised water and mixing on a Vortex shaker. The solution was then transferred to an amber vial. In these studies, typically no additives or stabilizers were used. Standard degradation studies undertaken at short term used the known method of "forced" temperature degradation by storing solutions at 70°C for 6 hours (to indicate general longer- term storage performance).
• NAC N-acetylcysteine
• CaM Calmangafodipir
• Calmangafodipir-related substances The concentration of NAC remained constant during the test, suggesting that this compound should be relatively stable in solutions with CaM.
• CaM showed a slight decrease in concentration (about 1 % per hour).
• T storage time
• Mixtures 1 and 3 described above are indicated as Aladote Test Mixture 1 and Aladote Test Mixture 3, respectively. Typical error bars are also shown.
• Fig. 5 shows diNAC formation versus storage time. The figures indicate: (a) NAC is stable in
• NAC:CaM Test Mixture 1 at both 4°C and 22°C (Fig. 1), (b) There is apparently some depletion in NAC in Test Mixture 3 at both 4°C (20% over 90 days) and at 22°C (30% over 90 days) (Fig. 2), (c) CaM is stable at both 22°C and 4°C storage for both Test Mixture 1 (Fig. 3) and Test Mixture 3 (Fig. 4), (d) Some of the depletion of NAC in Test Mixture 3, particularly at 22°C storage, appears to be due to diNAC formation (Fig. 5).
• This Example shows ALF response to acetaminophen-induced ALF mouse model studies based on accepted acetaminophen concentrations and methodologies.
• an i.p. injection of 300 mg/kg acetaminophen was made to male B6C3F1 mice to cause acetaminophen-induced ALF.
• a 300 mg/kg dosage of NAC was administered 1-6 hours after acetaminophen administration.
• NAC was administered in a dosage of 30 - 300 mg/kg NAC 1 hour after acetaminophen administration.
• a dosage of 0.3 - 10 mg/kg calmangafodipir (CaM) was adminisntered 6 hours post acetaminophen administration.
• ALAT was meausured 12 hours following the i.p. injection of 300 mg/kg acetaminophen.
• Figs. 6 to 8 show ALF response to administration of NAC and CaM, as detected by monitoring ALAT.
• NS refers to statistically not significantly different from control data while *** refers to the data being p ⁇ 0.001 , and * refers to the data being p ⁇ 0.05, statistically significant from control data.
• FIG. 7 indicates the NAC dose-response of NAC administration of 30 - 300 mg/kg NAC 1 hour post acetaminophen administration.
• Mean ⁇ SEM ALAT levels at 12 hours following i.p. injection of 300 mg/kg acetaminophen in male B6C3F1 mice (n 4 - 1 1 per bar) are shown. No clear dose-response could be detected.
• Fig. 8 shows the CaM dose response of administration of 0.3 - 10 mg/kg calmangafodipir (CaM) 6 hours post acetaminophen administration of 300 mg/kg acetaminophen. Median ⁇ interquartile AL.AT levels at 12 hours following i.p.
• This Example studied the pharmaceutical effects of individual and combined therapies on animal deaths and involved male B6C3F1 mice. Even though serum enzyme activity studies are strongly indicative of liver failure or protection, it is also important to consider other data such as that related to subject animal deaths (%) as a function of the
• mice were fasted for 8-10 hours and ALF was induced with 300 mg/kg acetaminophen (APAP), i.p., with treatment as specified in the following test groups:
• APAP acetaminophen
• Table 1 shows the relative number of deaths (%) within the 12 hour sampling period as a function of the pharmacological treatment. The highest number of deaths was seen in the chelator group without a manganese component (CaDPDP). Surprisingly, a lower number of deaths than expected was seen for the combination of NAC and CaM, compared with either NAC or CaM alone or with the APAP control.

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