WO2017080668A1

WO2017080668A1 - Pharmaceutical preparation effective in age-related disorders

Info

Publication number: WO2017080668A1
Application number: PCT/EP2016/001887
Authority: WO
Inventors: Peter Wehling; Julio Reinecke
Original assignee: Orthogen Ag
Priority date: 2015-11-13
Filing date: 2016-11-14
Publication date: 2017-05-18
Also published as: TW201722442A; TW201722443A; WO2017080669A1

Abstract

The present invention relates a pharmaceutical preparation for the treatment of age- related disorders, which is preparable by a production method comprising the following steps: providing a liquid collected from an organism, which liquid comprises cellular constituents of blood, and a vessel or containment means and contacting said liquid with said vessel or containment means, wherein (i) said production method further comprises the step of incubating said liquid in said vessel or containment means, and optionally removing cellular constituents of said liquid after said incubation, (ii) said liquid comprises exosomes, and said production method further comprises the steps of concentrating said exosomes and optionally removing cellular constituents of said liquid after said concentration, or the step of isolating said exosomes, or (iii) said production method further comprises the step of avoiding incubation of said liquid, and the step of removing cellular constituents of said liquid contacted with said vessel or containment means.

Description

ORTHOGEN AG

Ernst-Schneider-Platz 1 , 40212 Dusseldorf

"Pharmaceutical preparation effective in age-related disorders"

The present invention relates to the field of ageing, in particular to the treatment of age-related disorders and provides a pharmaceutical preparation for such treatment.

Managing ageing of the human population and ageing-associated disorders is a global challenge. Many industrialised countries have an increasingly ageing population, and also in developing countries the proportion of elderly people is expected to rise steeply. On a biological level ageing may be defined as a progressive deterioration over time of an organism and its individual cells, tissues and organs. Such deterioration may lead to certain age-related disorders. It has been estimated that by 2050 the number of people aged 60 and older worldwide will approximately double as compared to its current figure of around 1 1 %.

Several theories of ageing and associated frailty have been proposed. One theory focuses on a progressive accumulation of damage. Longevity and health at an advanced age appear to be influenced, amongst other factors, by a balance between damage caused to biomolecules (in particular DNA) and maintenance and repair systems. Therefore ageing has been proposed to be correlated to DNA damage, and certain age-associated disorders might be due to an excess of DNA damage or an impaired DNA repair. Therefore corresponding animal models of ageing have been developed, and widespread models include mice that have defects in Nucleotide Excision Repair (NER) and show a premature ageing phenotype as compared to wild-type mice. It is believed that the alterations in such mice and the accompanying disorders reflect normal ageing in mice, but also in other organisms such as humans. Oxidative damage to cells and cell components has also been implicated in ageing and certain disorders, and thus the lack of sufficient repair, such as the impaired expression or activity of antioxidant enzymes. Cellular senescence has also been linked to the shortening of telomeres, which may act as a molecular clock and thus might be the cause of certain disorders linked with senescence. Where cells become incapable of maintaining a sufficient rate of cell division, a lack of renewal of tissues might promote age-related disorders. Excessive cell death such as apoptosis may also contribute to ageing, senescence and associated disorders. Another ageing theory focuses on excessive (even if subtle) inflammation, which exerts harmful effects in advanced age. The immune system is also subject to senescence, one characteristic being "immunosenescence" associated with a reduced sensitivity to vaccination.

Ultraviolet (UV) radiation is inter alia present in sunlight. It has a wavelength below that of visible light. Biologically, the UV-A band (defined by ISO-21348 as 315 to 400 nm), the most long-wave part of the UV spectrum, and the neighbouring UV-B band (280 to 315 nm) are most important, whereas UV radiation having a shorter wavelength (UV-C) is practically absorbed by the ozone layer and the earth's atmosphere. UV radiation is able to trigger chemical reactions. Whereas it has certain beneficial actions on the human body, it is also dangerous since it causes damage, in particular to the skin and the eyes. UV radiation (all bands) is known to damage collagen and elastin fibres, which gives rise to skin ageing (photoageing), whose signs may include loose skin, dryness and/or wrinkling.

In the DNA, UV-B radiation causes the formation of pyrimidine dimers (in particular thymine dimers), a process that is also known as direct DNA damage. The formed lesions alter the DNA structure and may be repaired by a mechanism known as Nucleotide Excision Repair. If unrepaired, the lesions are mutagenic. UV radiation also causes the production of reactive oxygen species/free radicals, which gives rise to oxidative damage, a process known as indirect DNA damage. Both direct and indirect DNA damage contribute to the formation of cancer. It is therefore apprehensible that broad-spectrum UV radiation is recognised as a carcinogen by the World Health Organisation.

One of the features of the immune system is an interplay between T cells and macrophages. In this regard, two major types of immune processes are commonly distinguished, Type 1 and Type 2. In the Type 1 processes, Type 1 T helper cells (T_h1 cells) and Type 1 macrophages (M1) are involved, and these processes play a major role in the cellular immune response and pathophysiological^ in inflammatory processes. The Type 2 processes involve Type 2 T helper cells (T_h2 cells) and Type 2 macrophages (M2) and play a role in anti-inflammatory and/or regenerative processes such as wound healing and tissue repair, besides their role in the humoral immune response. Typical cytokines associated with Type 1 processes are IFN-γ and IL-2. Type 1 immune response maximise the cellular killing ability. Where there is a (chronic) preponderance of Type 1 immune processes, damage to the organism may occur, e.g. when directed to autoantigens type 1 diabetes, and more generally ageing may be accelerated due to excessive and/or chronic inflammation. The preponderance of Type 1 immune processes may e.g. be a preponderance of Type 1 over Type 2 immune processes. Such an imbalance in immune processes might be countered by promoting other immune processes (e.g. Type 2) or inhibiting Type 1 immune processes, facilitating the resolution of inflammation.

In neurodegeneration, such as in Alzheimer's disease, Parkinson's disease and multiple sclerosis a role of macrophages (such as microglia) has been described. Shifting the balance from Type 1 immune responses to Type 2 immune responses might be particularly interesting in nervous system or skin disorders. Cellular senescence is a phenomenon that leads to the inability of isolated cells to divide perpetually and makes them arrest after a certain number of divisions. For the detection of cellular senescence a cellular staining assay that detects senescence-associated β- galactosidase activity is commonly used (see e.g. Dimri et al. (1995) PNAS 92, 9363 to 9367). Cells exhibiting a senescent phenotype may interfere with vital functions of a whole organism and thus lead to certain disorders. The senescence of a whole organism is accompanied by an increased risk of certain disorders (such as diseases, complications and conditions). It is found that the incidence of certain disorders increases with age in the greater than linear fashion, for example exponentially. Common specific disorders correlated with age are atherosclerosis, cardiovascular disease, cancer, hearing deficits, vision deficits, cataracts, retinal degeneration, e.g. macular degeneration, osteoporosis, type 2 diabetes, hypertension, liver failure, cachexia, kyphosis, gait disorders, tremors, ataxia, dystonia, reduced grip strength, muscle wasting and hair greying. Age also promotes neurodegeneration and similar disorders, such as mild cognitive impairment, Alzheimer's disease, cerebrovascular disease, Parkinson's disease and amyotrophic lateral sclerosis.

It is a widespread desire to extend the lifespan and/or the healthspan, i.e. the life period during which one is generally healthy and free from serious disease. Whereas a few compounds or compositions have been suggested for this purpose or for the treatment of the above-mentioned disorders in the prior art, their efficacy and/or tolerability is not always clear. Thus there is a need for alternative treatments.

It is the problem of the present invention to provide a novel means for treating, preventing, inhibiting or mitigating one or more of the above-mentioned disorders, or for interfering with one or more of the biological causes of such disorders. Advantageously, such a means is fast and easy to produce and is cost effective. Also advantageously such a means should have good body compatibility. The above statements, and any description of exemplified embodiments herein, do not constitute any waiver of certain embodiments or features.

This problem is solved by a pharmaceutical preparation for use in the treatment of

(a) a disorder caused by oxidative damage, DNA damage, impaired DNA repair, impaired cell division, excessive inflammation, a pathogenic polarisation of immune processes

(preferably a preponderance of Type 1 immune processes), or excessive cell death, or

(b) a disorder selected from the group consisting of kyphosis, osteoporosis, tremor, ataxia, dystonia, neurodegeneration, neuroinflammation, Alzheimer's disease, mild cognitive impairment, cerebrovascular disease, Parkinson's disease, amyotrophic lateral sclerosis, a gait disorder, and reduced grip strength, muscle wasting and cachexia and hair greying, or

(c) a disorder that is mimicked by a disorder of a genetically altered mouse that has at least one mutation in a gene encoding a protein of the Nucleotide Excision Repair pathway, said mutation causing a premature ageing phenotype as compared to a mouse lacking said mutation, or

(d) an age-related disorder or a disorder whose incidence increases with age in a greater than linear fashion, or

(e) a disorder caused by collagen damage and/or elastin damage, senescence, telomere shortening, impaired expression of antioxidant enzymes or impaired activity of antioxidant enzymes, or

(f) a disorder selected from the group consisting of atherosclerosis, cardiovascular disease, cancer, hearing deficits, vision deficits, cataracts, retinal degeneration, type 2 diabetes, hypertension and liver failure,

wherein the pharmaceutical preparation is preparable by a production method comprising the following steps: providing a liquid collected from an organism, which liquid comprises cellular constituents of blood, and a vessel or containment means and contacting said liquid with said vessel or containment means, wherein

(i) said production method further comprises the step of incubating said liquid in said vessel or containment means, and optionally removing cellular constituents of said liquid after said incubation,

(ii) said liquid comprises exosomes, and said production method further comprises the steps of concentrating said exosomes and optionally removing cellular constituents of said liquid after said concentration, or the step of isolating said exosomes, or

(iii) said production method further comprises the step of avoiding incubation of said liquid, and the step of removing cellular constituents of said liquid contacted with said vessel or containment means.

In the following said production method (with its alternatives (i), (ii) and (iii)) is sometimes referred to as "the production method of the present specification".

The use in the treatment of the above-mentioned is in the following sometimes referred to as "the use according to the present invention". Accordingly the pharmaceutical preparation for use in the treatment of the above-mentioned disorders is sometimes referred to as "the pharmaceutical preparation for use according to the present invention"

The present invention may also be described as a method of treating

(a) a disorder caused by oxidative damage, DNA damage, impaired DNA repair, impaired cell division, excessive inflammation, a pathogenic polarisation of immune processes (preferably a preponderance of Type 1 immune processes), or excessive cell death, or

(b) a disorder selected from the group consisting of kyphosis, osteoporosis, tremor, ataxia, dystonia, neurodegeneration, neuroinflammation, Alzheimer's disease, mild cognitive impairment, cerebrovascular disease, Parkinson's disease, amyotrophic lateral sclerosis, a gait disorder, and reduced grip strength, muscle wasting and cachexia and hair greying, or (c) a disorder that is mimicked by a disorder of a genetically altered mouse that has at least one mutation in a gene encoding a protein of the Nucleotide Excision Repair pathway, said mutation causing a premature ageing phenotype as compared to a mouse lacking said mutation, or

in a patient in need thereof, comprising administering to said patient a pharmaceutical preparation which is preparable by a production method comprising the following steps: providing a liquid collected from an organism, which liquid comprises cellular constituents of blood, and a vessel or containment means and contacting said liquid with said vessel or containment means, wherein

"Disorders" refers to disturbances in normal function or appearance and includes diseases, complications and conditions.

A "cellular constituent of blood" means any cellular constituent of whole blood, whether present in large or small amounts.

Preferably the "liquid" is a blood sample, which is (1) a whole blood sample or (2) a whole blood sample from which cells have been depleted. In this context, the cells that have been depleted are preferably selected from erythrocytes, leukocytes (in particular neutrophils, eosinophils, basophils, lymphocytes, B cells, T cells, NK cells and/or monocytes) and platelets. More preferably the cells that have been depleted are erythrocytes.

"Containment means" refers to one or more containment means (preferably "container"). The vessel or containment means may be the same as that/those or different than that/those in which said liquid has been collected. "Incubation" or "incubating" preferably refers to (i) an incubation with a duration of at least 2 min, 3 min, 4 min, 5 min, 7 min, 10 min, 15 min, 20 min, 25 min, 30 min, 35 min, 40 min, 45 min, 50 min, 55 min, 1 h, 1.5 h, 2 h, 2.5 h, 3 h, 3.5 h, 4 h, 4.5 h, 5 h, 5.5 h or 6 h and/or (ii) an incubation after which a measurable change in a certain parameter has occurred with respect to the value before incubation and/or the normal value of that parameter (such as the concentration of a cytokine, the concentration of a cytokine antagonist, in particular IL-1 Ra, the concentration of exosomes and/or the concentration of a growth factor).

Where embodiments of the present invention are described as "containing" or "comprising" certain subject matter, e.g. methods steps, constituents or other features, it is understood that preferred embodiments consist of said subject matter, except where the context dictates otherwise.

According to a preferred embodiment, the present invention therefore relates to a pharmaceutical preparation for use in the treatment of

(a) a disorder caused by oxidative damage, DNA damage, impaired DNA repair, impaired cell division, excessive inflammation, a preponderance of Type 1 immune processes, or excessive cell death, or

(f) a disorder selected from the group consisting of atherosclerosis, cardiovascular disease, cancer, hearing deficits, vision deficits, cataracts, retinal degeneration such as macular degeneration, type 2 diabetes, hypertension and liver failure,

wherein the pharmaceutical preparation is preparable by a production method comprising the following steps: providing a blood sample collected from an organism and a vessel or container, contacting said blood sample with said vessel or a container, and incubating said blood sample in said vessel or container, wherein said blood sample is (1 ) a whole blood sample or (2) a whole blood sample from which erythrocytes have been depleted.

This embodiment may also be described as a method of treating

(a) a disorder caused by oxidative damage, DNA damage, impaired DNA repair, impaired cell division, excessive inflammation, a preponderance of Type 1 immune processes, or excessive cell death, or (b) a disorder selected from the group consisting of kyphosis, osteoporosis, tremor, ataxia, dystonia, neurodegeneration, neuroinflammation, Alzheimer's disease, mild cognitive impairment, cerebrovascular disease, Parkinson's disease, amyotrophic lateral sclerosis, a gait disorder, and reduced grip strength, muscle wasting and cachexia and hair greying, or

in a patient in need thereof, comprising administering to said patient a pharmaceutical preparation which is preparable by a production method comprising the following steps: providing a blood sample collected from an organism and a vessel or container, contacting said blood sample with said vessel or a container, and incubating said blood sample in said vessel or container, wherein said blood sample is (1 ) a whole blood sample or (2) a whole blood sample from which erythrocytes have been depleted.

The following statements apply irrespective of whether the present invention is described as a pharmaceutical preparation for use in a certain treatment, or as a method of treating.

It is preferred that said liquid is a blood sample, which is a whole blood sample, according to the above alternative (1).

With regard to the above item (a), preferably said oxidative damage is damage by reactive oxygen species. Said disorder caused by DNA damage is preferably a disorder caused by Independent DNA damage. Preferably said disorder caused by UV-dependent DNA damage is selected from a disorder caused by pyrimidine dimers, basal-cell carcinoma, squamous-cell carcinoma and melanoma. It is preferred that said disorder caused by impaired DNA repair is a disorder caused by deficient Nucleotide Excision Repair. Preferably said disorder caused by impaired cell division is a disorder associated with (more preferably caused and/or or mimicked by) impaired division of nucleus pulposus cells. In another preferred embodiment said disorder caused by excessive inflammation is a non-orthopaedic disorder, a disorder not involving the nervous system and/or a disorder not involving the eye. It is preferred that said disorder caused by excessive inflammation is different from one, more than one, or all of the following disorders: rheumatism, arthritis, rheumatoid arthritis, juvenile rheumatoid arthritis, psoriatic arthritis, Bechterew arthritis, osteoarthritis, back symptoms, neuroorthopaedic disorders, joint disorders, intervertebral disc disorders, spinal disorders, nerve root disorders, tendon disorders, loss of cartilage, neurodermitis and alopecia. Preferably said Type 1 immune processes are T_h1 and/or M1 processes, and more preferably they are T_h1 and M1 processes. "Preponderance of Type 1 immune processes" includes the meaning "preponderance of Type 1 over Type 2 immune processes", wherein said Type 2 immune processes are preferably T_h2 and/or 2 processes and more preferably T_h2 and M2 processes. "Preponderance of Type 1 immune processes" also includes the meanings "excessive Type 1 immune processes" and "impaired other immune processes", e.g. "impaired Type 2 immune processes" (with preferred embodiments as set out above). Said cell death is preferably apoptosis.

With regard to the above item (c), said mutation in said genetically altered mouse is preferably in the Ercd gene. More preferably it is Ercc1^"'^" or Ercc1^{" A}.

With regard to the above item (d), preferably said incidence increases exponentially with age.

With regard to the above item (e), preferably said disorder caused by collagen damage and/or elastin damage is selected from loose skin, dryness and wrinkling. A disorder caused by collagen damage and/or elastin damage may also be generally described as skin ageing.

It has now surprisingly been found that a preparation prepared according to the production method of the present specification counteracts the harmful effects of UV radiation on cells, has a stronger anti-inflammatory effect in older patients than in younger patients, contains an even more favourable ratio anti-inflammatory to inflammatory components in older patients than in younger patients, induces a shift in immune system function from inflammatory to regenerative and anti-inflammatory, stimulates an anti-apoptotic pathway and increases cell division, which serves as the basis of the present invention. The preparation prepared according to the production method of the present specification is thus a pharmaceutical preparation, and it may be used as a novel means for treating, preventing, inhibiting or mitigating the above-mentioned disorders or in interfering with the biological causes of the aforementioned.

The present invention further provides a pharmaceutical preparation for use in the treatment a disorder selected from the group consisting of Lichen sclerosus et atrophicus (LSA), Ehlers- Danklos Syndrome, Elastosis actinica, Elastoidosis cutanea nodularis et cystica and Elastosis perforans serpiginosa. In these disorders elasticity of the skin and/or the connective tissue is impaired. The pharmaceutical preparation for use according to the present invention is preparable by a production method which comprises: providing a liquid collected from an organism, which liquid comprises cellular constituents of blood, and a vessel or containment means and contacting said liquid with said vessel or containment means, wherein (i) said production method further comprises the step of incubating said liquid in said vessel or containment means, and optionally removing cellular constituents of said liquid after said incubation, (ii) said liquid comprises exosomes, and said production method further comprises the steps of concentrating said exosomes and optionally removing cellular constituents of said liquid after said concentration, or the step of isolating said exosomes, or (iii) said production method further comprises the step of avoiding incubation of said liquid, and the step of removing cellular constituents of said liquid contacted with said vessel or containment means. After carrying out these method steps, a pharmaceutical preparation is present, and its efficacy results from carrying out these method steps. Preferably said production method comprises: providing a blood sample collected from an organism and a vessel or container, contacting said blood sample with said vessel or a container, and incubating said blood sample in said vessel or container. It then results in a conditioned blood sample. For example during the incubation step, efficacious components are induced in the liquid (preferably blood sample), in particular due to the activity of cells therein. Therefore preferably said liquid is a blood sample, which is a whole blood sample. However, the blood sample may also be a fraction of whole blood. For example erythrocytes, being cells that lack a nucleus and thus gene expression ability, may be absent from the blood sample. Therefore said blood sample is alternatively a whole blood sample from which erythrocytes have been depleted (preferably completely or substantially completely, but it is alternatively envisaged that only part of the erythrocytes have been depleted), for example a buffy coat or PRP (platelet-rich plasma). It is unnecessary to, and preferred not to, add any external stimulators or activators. Before the providing step, the production method of the present specification preferably additionally includes the step of collecting said liquid (preferably blood sample, more preferably whole blood sample) from said organism.

Said organism may or may not suffer from any of the above-mentioned disorders and cells in that organism may or may not exhibit the above-mentioned phenotype.

The pharmaceutical preparation for use according to the present invention has been found to counteract the harmful effects of UV radiation on cells. The cell count in cells irradiated with a combination of UV-A and UV-B radiation has been found to be higher in the presence of the pharmaceutical preparation for use according to the present invention than in its absence. The magnitude of this effect was dependent on the incubating step. Therefore incubation of the liquid (preferably blood sample, more preferably whole blood sample) leads to the formation of components efficacious in promoting the survival and/or proliferation of UV-irradiated cells.

In this respect the effect of the pharmaceutical preparation for use according to the present invention, namely to rescue DNA damage caused by UV, is the same as that of a functional Nucleotide Excision Repair system. Therefore, without being bound to theory it may be concluded that the pharmaceutical preparation for use according to the present invention stimulates Nucleotide Excision Repair. More generally, it may be concluded that the pharmaceutical preparation for use according to the present invention has an effect on a disorder caused by impaired DNA repair or accumulation of DNA damage.

Accordingly it may be assumed that the pharmaceutical preparation for use according to the present invention is useful in treating a disorder caused by insufficient Nucleotide Excision Repair. Several models exist that are deficient in Nucleotide Excision Repair, for example Ercd^" mice. This model shows a premature ageing phenotype and is considered to reflect normal ageing, both in mice and in other organisms, such as humans. It shows age related pathologies in coat condition, kyphosis, gait, tremors, ataxia, dystonia and grip strength. Kyphosis has further implications on osteoporosis. Ataxia, dystonia and tremors are neurodegenerative disorders and have an impact on neurodegeneration and associated disorders like Alzheimer's disease, mild cognitive impairment, cerebrovascular disease, Parkinson's disease and amyotrophic lateral sclerosis. Gait and grip strength have an impact on muscle wasting. Moreover, neurodegeneration and associated disorders are usually linked to neuroinflammation. It can be concluded that the anti-inflammatory action of the pharmaceutical preparation for use according to the present invention is also useful in the treatment of neuroinflammation, neurodegeneration and associated disorders.

Moreover, the pharmaceutical preparation for use according to the present invention stimulates the NF-KB pathway, which is known to have anti-apoptotic effects, in particular through inducing the expression of a number of genes whose products can inhibit apoptosis (see e.g. Karin & Lin (2002) Nat Immunol 3, 221). Therefore the conclusion may be drawn that it can be used in the treatment of a disorder caused by excessive cell death.

The pharmaceutical preparation for use according to the present invention has also been found to increase cell division. Accordingly it may be concluded that it has an effect on a disorder caused by impaired cell division. Since senescence and ageing may be caused by impaired cell division, this finding may contribute to explaining the general effect of the pharmaceutical preparation in age-related disorders. For example, the increased division of cells of the intervertebral disc (nucleus pulposus) may rejuvenate the intervertebral discs and may help prevent disorders of the intervertebral discs such as slipped discs in which a lack of sufficient cell division and renewal may play a role.

The pharmaceutical preparation for use according to the present invention may afford, for example, a simple, cost effective and/or rapid production. By carrying out a series of easy steps in the production and without the need for special or complicated equipment and materials, in a minimum of steps and a few hours, a ready-to-use pharmaceutical preparation is realised without having to add any substances foreign to the body during production or such other substances which will have to separated again later in the production. By using exclusively a body's own substances, in this manner, an especially body-compatible agent is produced.

It is known that whole blood, and conditioned whole blood, contains exosomes. Exosomes are small vesicles secreted from cells into their environment. Exosomes are for example contained in biological liquids such as serum, urine, saliva, peritoneal-, cerebrospinal- and synovial liquids. Most types of cells looked at are able to secrete exosomes. The secretion occurs through release through/from the cell's plasma membrane. Depending on the cell type in which they are generated, exosomes contain inter alia a variable combination of proteins. In the following, the term "exosomes" preferably additionally comprises other extracellular vesicles (EV).

Conditioning, as described above, leads to additional formation of exosomes and other applications substances. However, it is not always necessary to condition a liquid (preferably blood sample). Since exosomes have been surprisingly found by the present inventors to exhibit a number of relevant effects (such as proliferation of cells in culture, drop in systemic CRP levels), exosomes may be concentrated or isolated after conditioning, but alternatively exosomes existing in the blood may be concentrated or isolated without conditioning in order to achieve beneficial effects.

Accordingly, with regard to alternative (ii) of the production method of the present specification, it is preferred that the production method, before the step of concentrating or isolating said exosomes, further comprises the step of incubating said liquid (preferably blood sample) in said vessel or containment means for an incubation time, or the step of avoiding incubation of said liquid (blood sample). Concentrating or isolating is useful to further increase the efficacy. Since also in the absence of incubation the pharmaceutical preparation prepared according to the production method of the present specification has been surprisingly found by the present inventors to exhibit a number of relevant effects (such as promoting cell survival after UV irradiation), incubation may be avoided in certain embodiments, in accordance with alternative (iii) of the production method of the present specification. In this alternative, the step of removing cellular constituents of the liquid (preferably blood sample) is always carried out. Such a step of removing cellular constituents is often beneficial in all alternatives of the production method of the present specification.

With regard to all alternatives of the production method of the present specification, the step of removing cellular constituents is preferably a step of removing the erythrocytes (in particular in the case when said liquid comprising cellular constituents of blood is whole blood), the platelets (in particular in the case when said liquid comprising cellular constituents of blood is whole blood or whole blood from which erythrocytes have been depleted) or the entirety of cellular constituents (in particular in the case when said liquid comprising cellular constituents of blood is whole blood or whole blood from which erythrocytes have been depleted. It is most preferred that the step of removing cellular constituents is a step of removing the entirety of cellular constituents in the case when said liquid comprising cellular constituents of blood is whole blood). Such a separation may be achieved by centrifugation, such as a short centrifugation at a low relative centrifugal force (e.g. about 10 minutes at 1000 g) or by filtration.

In alternatives (i) and (ii) of the production method of the present specification, the step of removing cellular constituents of said liquid is optional, and alternatively it is also envisaged to include a step of refraining from removing cellular constituents of said liquid (or refraining from removing those cellular constituents with specific desired functions). In particular it is envisaged to refrain from removing the erythrocytes, the platelets or the entirety of cellular constituents.

Preferably the production method of the present specification further comprises the step of reducing the volume of said liquid and/or the pharmaceutical preparation prepared according to the production method of the present specification is in dry form (in particular powder form).

In order to achieve the maximum effect, it is preferred to avoid storage before using the pharmaceutical preparation preparable according to the production method of the present specification. Preferably the organism referred to above in the context of the pharmaceutical preparation for use according to the present invention is a human being. Preferably said human being is at least 48, 50, 55, 60 or 65 years old. Also envisaged is an organism that is an animal, such as a dog, a cat, a horse or a camel.

It is known that conditioned whole blood contains exosomes. Exosomes are small vesicles secreted from cells into their environment. Exosomes are for example contained in biological liquids such as serum, urine and synovial liquids. Most types of cells are able to secrete exosomes. The secretion occurs through release from the cell's plasma membrane. Depending on the cell type in which they are generated, exosomes contain inter alia a variable combination of proteins.

In the following, the term "exosomes" preferably additionally comprises other extracellular vesicles (EV).

Preferably the pharmaceutical preparation for use according to the present invention comprises exosomes.

Preferably exosomes contained in the pharmaceutical preparation for use according to the present invention have been generated during the above-mentioned incubation, if any, of the liquid (preferably blood sample, more preferably whole blood sample) collected from the organism in a vessel or containment means.

The average diameter of the exosomes in an exosome-containing pharmaceutical preparation for use according to the present invention, as established by means of a transmission electron microscope, is preferably between 30 and 200 nm, in particular between 50 and 190 nm, between 70 and 180 nm, between 90 and 160 nm or between 100 and 150 nm. Exosomes of this size are the basis for an especially high efficacy, while larger vesicles represent essentially damaged exosomes and aggregates.

The production method of the present specification preferably further comprises the step of concentrating or isolating the exosomes after the incubation in order to increase its efficacy further. Such a concentrating or isolating step may lead to two or more pharmaceutical preparations prepared according to the production method of the present specification. These two or more pharmaceutical preparations correspond to the fractions obtained after performing such a concentrating or isolating step. Concentrating or isolating the exosomes may for example be realised through centrifugation at 100,000 g, as such strong accelerations are especially suitable to concentrate or isolate exosomes. Such a centrifugation is preferably conducted for at least 30 min, especially for at least 60 min. The pellet formed by the centrifugation then contains the exosomes. Preferably the concentrated or isolated exosomes are then taken up in a fluid (preferably a buffered solution such as PBS). Optionally they are then filtrated, for example through a 0.2 μηι filter.

The present invention also conceives a pharmaceutical preparation for use according to the present invention that does not contain exosomes. However, a preferred pharmaceutical preparation for use according to the present invention does comprise exosomes, and in particular it may consist of exosomes.

In case serum or plasma is contained in the containing pharmaceutical preparation for use according to the present invention (which is preferred in certain cases), such serum or plasma preferably contains cytokines and/or growth factors.

Preferably the pharmaceutical preparation for use according to the present invention does not comprise a corticosteroid, since this may impair the efficacy of the pharmaceutical preparation, in particular due to inhibition of its senescence-rescuing effect and/or anti-apoptotic effect.

According to a preferred embodiment the production method of the present specification comprises the following step: removing the cellular constituents of the liquid (preferably blood sample, more preferably whole blood sample) after the incubation, if any, and before administering the pharmaceutical composition. Such a separation may be achieved by centrifugation, such as a short centrifugation at a low relative centrifugal force (e.g. about 10 minutes at 1000 g) or by filtration.

Incubation of the liquid (preferably blood sample, more preferably whole blood sample) is preferably carried out for a period of up to 72 hours, in particular a time period of 1 to 48 hours, 2 to 24 hours, 3 to 12 hours, 4 to 10 hours, 5 to 8 hours or 6 hours.

The incubation is preferably carried out at a temperature of 0°C to 45°C, in particular at temperatures of 10°C to 43°C, 20°C to 41 °C, 30°C to 40°C, 35°C to 39°C, 36°C to 38°C or 37°C. These temperatures ensure best efficacy.

Suitable vessels or containment meansfor carrying out the production method of the present specification are for example hypodermic needles, tubes such as vacuum tubes, microtiter plates and transfusion bags. The vessel or containment meanspreferably comprise(s) a surface for contacting the liquid (preferably blood sample, more preferably whole blood sample), which surface may comprise glass, plastic, corundum or quartz or a combination of these. A preferred plastic is selected from the group consisting of polystyrene, polycarbonate, polyethylene and polypropylene. According to a preferred embodiment the vessel or containment means contain(s) macroscopic particles, microscopic particles or nanoparticles and during incubation the liquid (preferably blood sample) is in contact with said particles. For the purposes of the present application, macroscopic particles are defined as particles that are visible when viewed with the naked eye, microparticles are defined as particles that are too small to be visible when viewed with the naked eye but are visible when viewed with a microscope, and nanoparticles are defined as particles that are too small to be visible when viewed with a microscope (and that are preferably larger than 1 nm). Such particles serve the purpose of enlarging the surface for contacting the liquid (blood sample) and can have the shape of spheres, granulates, powder, gels or wool. Preferred materials are glass, plastic, corundum, quartz, gold and clay mineral (e.g. kaolin). Especially preferred are glass spheres. The surface of the particles can optionally be modified, for example by incubation with a caustic agent such as 50% v/v chromosulphuric acid with subsequent repeated rinsing. Conditioned serum is also known as Orthokine®, which is used as the pharmaceutical preparation for use according to the present invention in a preferred embodiment.

Preferably the pharmaceutical preparation for use according to the present invention is for use in the treatment of the same organism from whom said liquid (preferably blood sample, more preferably whole blood sample) has been collected. In this case it is an organism that suffers from one or more of the above-mentioned disorders. Thus the pharmaceutical preparation for use according to the present invention is preferably an autologous pharmaceutical preparation and not an allogeneic one, especially for reasons of safety. When describing the present invention as the above-mentioned method of treating a disorder or method of rescuing a phenotype, this means that the organism and the patient are identical.

The pharmaceutical preparation for use according to the present invention may be administered locally or systemically. Suitable ways of administration include parenteral, in particular intravenous, intra-arterial, intramuscular, intra-articular, epi/peridural, subcutaneous, intra-peritoneal and topical.

In a preferred embodiment the pharmaceutical preparation for use according to the invention is for use is in a combination therapy with another agent effective in the treatment of one or more of the disorders mentioned above.

Examples

Example 1 : Proliferation of cells of nucleus pulposus origin The proliferation of nucleus pulposus cells was tested in the presence of various supplements: Human conditioned serum ("ACS"), which is a pharmaceutical preparation prepared according to the production method of the present specification, with and without exosomes; foetal bovine serum ("FBS") with and without exosomes; and exosomes isolated from ACS and from FBS (see Figure 1 ).

Cells of nucleus pulposus origin were plated in a 96 well plate (8000 cells per well) with DMEM/F12 medium and PenStrep 1 %. Cell culture medium was supplemented with full ACS with exosomes ("ACS+Ex"), ACS without exosomes ("ACS-Ex"), exosomes from ACS ("ExA"), full FBS with exosomes ("FBS+Ex"), FBS without exosomes ("FBS-Ex") and exosomes from FBS ("ExF"). Cell division was assessed photometrically after 24 h with an XTT assay in an ELISA reader. Exosomes were separated from ACS and FBS by 2 h centrifugation of 30 ml ACS and 30 ml FBS, respectively, at 100,000 g and resuspension of pellet in 3 ml of PBS each.

It was found that both ACS and FBS promoted cell division. Cells divided best with lower ACS concentrations (1 % to 2%). At higher concentrations of ACS (5%, 10%), both in the presence and in the absence of exosomes, an apparent decrease in cell number was observed, but this may reflect detachment of cells or cell conglomerates when cell culture medium was washed off and then replaced by XTT staining medium. This may result in false readings because only attached cells can contribute to the signal observed in this XTT assay. The highest proliferation was seen in the presence of ACS with exosomes. FBS showed no comparable apparent decrease in cell number at higher concentrations such as 5% and 10%, but was less efficient overall in promoting cell division. In the experiments performed with FBS, exosomes did not have a major effect on cell proliferation. Isolated exosomes of FBS origin and of ACS origin promoted cell division dose-dependently.

Consequently the pharmaceutical preparation preparable according to the production method of the present specification increases cell division.

Example 2: Stimulation of an anti-apoptotic pathway

The stimulation the NF-κΒ pathway, which has an anti-apoptotic effect, was tested by using a GFP/Luciferase reporter system by using the following samples: a pharmaceutical preparation prepared according to the production method of the present specification, which did not comprise isolating or concentrating exosomes generated during the incubation, and wherein the pharmaceutical preparation was a serum and the incubation time was 6 h ("ACS") and a control using control serum for which the production method of the present specification was not carried out ("Control"). The NF-κΒ pathway was stimulated with various concentrations of IL-1 β. In this experiment cells of chondrocytic origin were genetically altered with a DNA construct consisting of a basic CMV promoter driving a luciferase gene and a preceding 4 fold binding motif (TRE) for NF-KB proteins. Any induction of the NF-κΒ pathway in these cells is therefore visible as an enhanced luciferase activity (relative light units (RLU)) which can be quantified.

The results are shown in the following table and in Figure 2.

In Figure 2, the left bar in each group represents the Control and the right bar represents ACS.

It is apparent that the NF-κΒ pathway was stimulated by IL-1 β in a dose-dependent manner. The pharmaceutical preparation prepared according to the production method of the present specification ("ACS") enhanced this stimulation, over the whole range of tested IL-1 β concentrations.

Thus it can be concluded that a pharmaceutical preparation preparable according to the production method of the present specification stimulates an anti-apoptotic pathway.

Example 3: UV radiation of cells

In the first series of experiments, nucleus pulposus cells were plated in 24 well plates in the absence of serum and in the presence of a pharmaceutical preparation prepared according to the production method of the present specification, which was in the form of a serum ("EOT"). EOT concentrations were 0.05%, 0.1 %, 0.2%, 0.5% and 1%. One part of the EOT samples was not incubated, but processed immediately ("EOT0"). The other part of the EOT samples was incubated for six hours ("EOT6").

Six hours after plating the cells, cells were irradiated with UV light (Herolab transilluminator FT- 28/312, 6 tubes cat. nr. 29 84 100, 15 W, 312 nm). The emission maximum was 312 nm. The duration of irradiation was 80 s. Cells were grown for another 2 days at 37°C, 5% C0₂. Cells were detached with 500 μΙ of 1 % trypsin and counted in a Casy counter. The cell numbers per ml were as follows:

Additive UV no UV

no Serum 1.20E+03 5.92E+05

EOTO 2.00E+03 6.34E+05

0.05%

EOTO 0.1% 1.50E+03 5.23E+05

EOTO 0.2% 2.00E+03 9.74E+05

EOTO 0.5% 2.50E+03 1.48E+06 EOTO 1% 3.50E+03 1.00E+06

EOT6 1.50E+03 7.44E+05

0.05%

EOT6 0.1 % 1.20E+04 1.24E+06

EOT6 0.2% 8.00E+03 1.70E+06

EOT6 0.5% 1.00E+04 1.70E+06

EOT6 1% 1.35E+04 6.30E+05

A graph of the data is shown in Figure 3 A.

It was found that UV irradiation strongly decreased the cell count in the absence of serum. The presence of EOTO did not have a large effect. In contrast, the presence of EOT6 increased the cell counts by a factor of about 10, except for the lowest concentration of 0.05%.

It may be concluded that the pharmaceutical preparation prepared according to the production method of the present specification counteracts the harmful effects of UV radiation. This effect was dependent on incubation, i.e. on the formation of efficacious components during the period of incubation.

A second series of experiments confirmed these results. In this series, the non-irradiated controls were omitted. The results (cells/ml) are shown in the following table and in Figure 3 B.

Again it was seen that the pharmaceutical preparation prepared according to the production method of the present specification counteracts the harmful effects of UV radiation, and that this effect was dependent on the incubation step.

Example 4: Drop in systemic CRP levels

Human subjects of different age groups (20 to 45 years, 48 to 58 years, 60 to 83 years) received intramuscular or intra-articular injections of the pharmaceutical preparation prepared according to the production method of the present specification, which comprised isolated exosomes. Exosomes were separated from the conditioned blood sample by 2 h centrifugation at 100,000 g and the pellet was resuspended in PBS. The volume of PBS was one tenth of the serum volume. The subjects were intramuscularly administered 1 ml in each case. The total number of subjects was 22. Subjects each received a single injection, and CRP levels were measured before and about two weeks after the injection.

In each case the level of C-reactive protein (CRP) was determined by high sensitivity CRP ELISA ("hsCRP").and compared to the level before the treatment. In order to be able to compare the results, the relative drop in CRP level was calculated in each case. It was found that the median relative drop was least pronounced in the 20 to 45 year age group, more pronounced in the 48 to 58 year age group and most pronounced in the 60 to 83 year age group.

The results are shown in Figure 4. It can be concluded that the pharmaceutical preparation prepared according to the production method of the present specification decreases inflammation, as shown by the CRP levels, and thus counteracts a possible cause of ageing. It does even more so in older patients than in younger patients. Example 5: Age-dependence of the ratio of anti-inflammatory to inflammatory components

It was examined whether the age of a human being has an influence on the composition of the pharmaceutical preparation prepared according to the production method of the present specification.

To this end, in a series of in vitro experiments blood samples were collected from human subjects and subjected to the production method of the present specification. The concentrations of IL-1 β and IL-1 Ra were determined after the incubating step. It was found that the concentration of IL-1 β (inflammatory component) after incubation was not statistically significant dependent on age. The results are shown in Figure 5 A (total number of subjects: 165).

In contrast, the concentration of IL-1 Ra (anti-inflammatory component) after incubation was higher in older patients than in younger patients. The results are shown in Figure 5 B (total number of subjects: 368).

The conclusion is that the pharmaceutical preparation prepared according to the production method of the present specification as an anti-inflammatory effect and thus counteracts a possible cause of ageing. This effect is even stronger in older patients than in younger patients, as shown by the fact that the concentration of IL-1 Ra after incubation increases with age, but the concentration of IL-1 β does not. Example 6: Shift in immune system function

Four human volunteers each received four intramuscular injections of 4 ml of the pharmaceutical preparation prepared according to the production method of the present specification, which was in the form of an Autologous Conditioned Serum (1x per week).

Whole blood was drawn from the subjects at week 0, week 2 and week 4 and subjected to in vitro testing: The production of IL-2 and IFN-γ in the whole blood were tested in vitro under 24 h stimulation with 5.7 pg/ml PHA. The CRP levels in blood were additionally determined by high sensitivity C-reactive protein ELISA ("hsCRP").

It was found that the injections led to a strong decrease of the capability of the whole blood to produce IL-2 and IFN-γ, as shown in the following table. hsCRP was decreased.

The decrease in Type 1 cytokines (IL-2 and IFN-γ) means that the injections had induced a shift of the immune system from inflammatory to regenerative/anti-inflammatory. Therefore an imbalance in immune processes due to a preponderance of Type 1 immune processes may be treated.

From this it can be concluded that the pharmaceutical preparation prepared according to the production method of the present specification counteracts a possible cause of ageing.

Claims

A pharmaceutical preparation for use in the treatment of

The pharmaceutical preparation for use according to alternative (b) of claim 1 , wherein said production method, before the step of concentrating or isolating said exosomes, further comprises the step of incubating said liquid in said vessel or containment means for an incubation time, or the step of avoiding incubation of said liquid.

3. The pharmaceutical preparation for use according to any of the above claims, wherein said liquid is a blood sample, which is (1) a whole blood sample or (2) a whole blood sample from which erythrocytes have been depleted.

4. The pharmaceutical preparation for use according to claim 3, wherein said cells that have been depleted are erythrocytes.

5. The pharmaceutical preparation for use according to any of the above claims, wherein the step of removing cellular constituents is a step of removing the erythrocytes, the platelets or the entirety of cellular constituents.

6. The pharmaceutical preparation for use according to any of the above claims, wherein

(a) said production method further comprises the step of reducing the volume of said liquid and/or

(b) the pharmaceutical preparation is in dry form.

7. The pharmaceutical preparation for use according to any of the above claims, wherein

(a) said oxidative damage is damage by reactive oxygen species, said disorder caused by DNA damage is a disorder caused by UV-dependent DNA damage, said disorder caused by impaired DNA repair is a disorder caused by deficient Nucleotide Excision Repair, said disorder caused by impaired cell division is a disorder associated with by impaired division of nucleus pulposus cells, said disorder caused by excessive inflammation is a non-orthopaedic disorder, a disorder not involving the nervous system and/or a disorder not involving the eye, said Type 1 immune processes are T_h1 and/or M1 processes, or said cell death is apoptosis, or

(c) said mutation in said genetically altered mouse is in the Ercd gene, or

(d) said incidence increases exponentially with age, or

(e) said disorder caused by collagen damage and/or elastin damage is selected from loose skin, dryness and wrinkling.

8. The pharmaceutical preparation for use according to claim 7, wherein

(a) said disorder caused by UV-dependent DNA damage is selected from a disorder caused by pyrimidine dimers, basal-cell carcinoma, squamous-cell carcinoma and melanoma, said Type 1 immune processes are T_h1 and M1 processes, or

(c) said mutation is Ercd^"'^" or Ercc1^{" A}.

9. The pharmaceutical preparation for use according to any of the above claims, wherein said organism is a human being, a dog, a cat, a horse or a camel.

The pharmaceutical preparation for use according to claim 9, wherein said human being at least 48 years old.

11. The pharmaceutical preparation for use according to any of the above claims, wherein said pharmaceutical preparation comprises exosomes.

12. The pharmaceutical preparation for use according to claim 11 , wherein exosomes have been generated during said incubation.

13. The pharmaceutical preparation for use according to claim 11 or 12, wherein the average diameter of the exosomes as determined by transmission electron microscope is in the range between 30 and 200 nm.

14. The pharmaceutical preparation for use according to any of claims 11 to 13, wherein said production method further comprises the step of concentrating or isolating said exosomes after said incubation, and optionally taking up the concentrated or isolated exosomes in a fluid.

15. The pharmaceutical preparation for use according to any of the above claims, which comprises serum or plasma.

16. The pharmaceutical preparation for use according to any of the above claims, wherein said production method further comprises the step of removing the cellular constituents of the blood sample after said incubation.

17. The pharmaceutical preparation for use according to any of the above claims, wherein said incubation is carried out

(a) during a time period of up to 72 hours and/or

(b) at a temperature from 0°C to 45°C.

18. The pharmaceutical preparation for use according to any of the above claims, wherein said vessel or containment means

(a) include(s) a surface for contacting the liquid (preferably blood sample) that comprises glass, plastic, corundum or quartz or a combination thereof and/or

(b) contain(s) particles selected from the group consisting of macroscopic particles, microscopic particles and nanoparticles, and wherein during said incubation the liquid (preferably blood sample) is in contact with said particles.

19. The pharmaceutical preparation for use according to claim 18, wherein said plastic is selected from the group consisting of polystyrene, polycarbonate, polyethylene and polypropylene.

20. The pharmaceutical preparation for use according to any of the above claims, wherein said use is

(a) in the treatment of the same organism from whom said blood sample has been collected and/or (b) in a combination therapy with another agent effective in the treatment of said disorder.

21. The pharmaceutical preparation for use according to any of the above claims, wherein said treatment is by local or systemic administration.