WO2017069606A3 - Thermolabile enzyme and method of preparing thereof - Google Patents

Thermolabile enzyme and method of preparing thereof Download PDF

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Publication number
WO2017069606A3
WO2017069606A3 PCT/MY2016/000069 MY2016000069W WO2017069606A3 WO 2017069606 A3 WO2017069606 A3 WO 2017069606A3 MY 2016000069 W MY2016000069 W MY 2016000069W WO 2017069606 A3 WO2017069606 A3 WO 2017069606A3
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WO
WIPO (PCT)
Prior art keywords
preparing
enzyme
strain
thermolabile enzyme
thermolabile
Prior art date
Application number
PCT/MY2016/000069
Other languages
French (fr)
Other versions
WO2017069606A2 (en
Inventor
Binti MD. Desa FAUZIAH
Syifa Binti Ismail NURUL
Shahira Binti Ruslan AIMI
Sufni Binti Abdul Rashid ALIANA
Binti Nasri NOORAMIRA
Original Assignee
Myenzyme Sdn Bdh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Myenzyme Sdn Bdh filed Critical Myenzyme Sdn Bdh
Publication of WO2017069606A2 publication Critical patent/WO2017069606A2/en
Publication of WO2017069606A3 publication Critical patent/WO2017069606A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

There is disclosed an enzyme having a denaturing temperature of 70°C and above, with an optimum pH of 6-9 and a method of production thereof. In one embodiment, the method comprising the steps of: selecting and isolating at least one strain of at least one microorganism by means of enrichment technique; culturing the strain of the selected and isolated microorganism; and obtaining the enzyme from the culture of the strain thereof.
PCT/MY2016/000069 2015-10-23 2016-10-20 Thermolabile enzyme and method of preparing thereof WO2017069606A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2015703831 2015-10-23
MYPI2015703831 2015-10-23

Publications (2)

Publication Number Publication Date
WO2017069606A2 WO2017069606A2 (en) 2017-04-27
WO2017069606A3 true WO2017069606A3 (en) 2017-06-01

Family

ID=58557445

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MY2016/000069 WO2017069606A2 (en) 2015-10-23 2016-10-20 Thermolabile enzyme and method of preparing thereof

Country Status (1)

Country Link
WO (1) WO2017069606A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002055679A2 (en) * 2001-01-10 2002-07-18 Novozymes A/S Thermostable lipolytic enzyme variant
EP1624056B1 (en) * 2004-08-02 2008-11-19 Universiti Putra Malaysia Method for producing a recombinant thermostable Geobacillus T1 lipase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002055679A2 (en) * 2001-01-10 2002-07-18 Novozymes A/S Thermostable lipolytic enzyme variant
EP1624056B1 (en) * 2004-08-02 2008-11-19 Universiti Putra Malaysia Method for producing a recombinant thermostable Geobacillus T1 lipase

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE GenBank [O] 20 March 2015 (2015-03-20), XP055386927, Database accession no. LC035464.1 *
DATABASE GenBank [O] 30 December 2014 (2014-12-30), XP055386929, Database accession no. KF803588.1 *
DATABASE GenBank [O] 30 June 2015 (2015-06-30), XP055386925, Database accession no. KR 027924.1 *
KHORAMNIA, ANAHITA ET AL.: "Thermostable lipase from a newly isolated Staphylococcus xylosus strain; process optimization and characterization using RSM and ANN", ELECTRONIC JOURNAL OF BIOTECHNOLOGY, vol. 13, no. 5, pages 1 - 16 *
LI, HEBIN ET AL.: "Characterization of thermostable lipase from thermophilic Geobacillus sp. TW 1", PROTEIN EXPRESSION AND PURIFICATION, vol. 42, no. 1, 31 March 2005 (2005-03-31), pages 153 - 159, XP027390235 *
ROYTER, MARINA ET AL.: "Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOLI and Caldanaerobacter subterraneus subsp. tengcongensis", EXTREMOPHILES, vol. 13, no. 5, 5 July 2009 (2009-07-05), pages 769 - 783, XP019742652 *

Also Published As

Publication number Publication date
WO2017069606A2 (en) 2017-04-27

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