WO2017058003A3 - Method of isolating mesenchymal stromal cells and applications for tissue engineering - Google Patents

Method of isolating mesenchymal stromal cells and applications for tissue engineering Download PDF

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Publication number
WO2017058003A3
WO2017058003A3 PCT/MY2016/050063 MY2016050063W WO2017058003A3 WO 2017058003 A3 WO2017058003 A3 WO 2017058003A3 MY 2016050063 W MY2016050063 W MY 2016050063W WO 2017058003 A3 WO2017058003 A3 WO 2017058003A3
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WO
WIPO (PCT)
Prior art keywords
stromal cells
applications
mesenchymal stromal
tissue engineering
isolating mesenchymal
Prior art date
Application number
PCT/MY2016/050063
Other languages
French (fr)
Other versions
WO2017058003A2 (en
Inventor
Anthony Lawrence
Original Assignee
Elixir Biopharma Sdn. Bhd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elixir Biopharma Sdn. Bhd. filed Critical Elixir Biopharma Sdn. Bhd.
Priority to CA3017103A priority Critical patent/CA3017103A1/en
Priority to SG11201806561UA priority patent/SG11201806561UA/en
Publication of WO2017058003A2 publication Critical patent/WO2017058003A2/en
Publication of WO2017058003A3 publication Critical patent/WO2017058003A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/09—Means for pre-treatment of biological substances by enzymatic treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Financial Or Insurance-Related Operations Such As Payment And Settlement (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)

Abstract

A method of isolating mesenchymal stromal cells from umbilical cord, foreskin, cornea or dental pulp whereby the action of collagenase is quenched such that the tissue sample is only partially digested.
PCT/MY2016/050063 2015-10-02 2016-09-30 Method of isolating mesenchymal stromal cells and applications for tissue engineering WO2017058003A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA3017103A CA3017103A1 (en) 2015-10-02 2016-09-30 Method of isolating mesenchymal stromal cells and applications for tissue engineering
SG11201806561UA SG11201806561UA (en) 2015-10-02 2016-09-30 Method of isolating mesenchymal stromal cells and applications for tissue engineering

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2015002493 2015-10-02
MYPI2015002493 2015-10-02

Publications (2)

Publication Number Publication Date
WO2017058003A2 WO2017058003A2 (en) 2017-04-06
WO2017058003A3 true WO2017058003A3 (en) 2017-05-04

Family

ID=58423898

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MY2016/050063 WO2017058003A2 (en) 2015-10-02 2016-09-30 Method of isolating mesenchymal stromal cells and applications for tissue engineering

Country Status (3)

Country Link
CA (1) CA3017103A1 (en)
SG (1) SG11201806561UA (en)
WO (1) WO2017058003A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200104300A (en) 2017-12-22 2020-09-03 키에시 파르마슈티시 엣스. 피. 에이. Mesenchymal stromal cells and method for obtaining mesenchymal stromal cells from umbilical cord
CN110577930A (en) * 2019-09-30 2019-12-17 重庆赛托斯创生物科技发展有限公司 Multi-connected-tube adipose-derived stem cell extraction method
CN112280735B (en) * 2020-09-16 2022-03-29 生物岛实验室 Umbilical cord-derived mesenchymal stem cells and preparation method and application thereof
CN113604430A (en) * 2021-09-06 2021-11-05 郑州源创吉因实业有限公司 Method for culturing dental pulp mesenchymal stem cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050242531A1 (en) * 2004-04-29 2005-11-03 Young Gary L Chuck with torque indicator
WO2012117333A1 (en) * 2011-02-28 2012-09-07 Stempeutics Research Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
KR101385374B1 (en) * 2012-10-29 2014-04-14 신현진 A jig for processing artificial tooth

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050242531A1 (en) * 2004-04-29 2005-11-03 Young Gary L Chuck with torque indicator
WO2012117333A1 (en) * 2011-02-28 2012-09-07 Stempeutics Research Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
KR101385374B1 (en) * 2012-10-29 2014-04-14 신현진 A jig for processing artificial tooth

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BANITALEBI DEHKORDI, MEHDI ET AL.: "A simple, rapid, and efficient method for isolating mesenchymal stem cells from the entire umbilical cord", CELL TRANSPLANTATION, vol. 25, no. 7, pages 1287 - 1297, XP055379699 *
BAYATI, VAHID ET AL.: "Expression of surface markers and myogenic potential of rat bone marrow-and adipose-derived stem cells: a comparative study", ANATOMY & CELL BIOLOGY, vol. 46, 2013, pages 113 - 121, XP055379706 *
VENUGOPAL, PARVATHY ET AL.: "Isolation, characterization, and gene expression analysis of Wharton' s jelly-derived mesenchymal stem cells under xeno-free culture conditions", STEM CELLS AND CLONING: ADVANCES AND APPLICATIONS, vol. 4, 2011, pages 39 - 50, XP055379704 *
VISHWANATH, VINAY RAO ET AL.: "Differentiation of isolated and characterized human dental pulp stem cells and stem cells from human exfoliated deciduous teeth: An in vitro study", JOURNAL OF CONSERVATIVE DENTISTRY, vol. 16, no. 5, 2013, pages 423 - 428 *

Also Published As

Publication number Publication date
SG11201806561UA (en) 2018-09-27
WO2017058003A2 (en) 2017-04-06
CA3017103A1 (en) 2017-04-06

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