WO2017047856A1 - Bande de biocapteur et son procédé de fabrication - Google Patents

Bande de biocapteur et son procédé de fabrication Download PDF

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Publication number
WO2017047856A1
WO2017047856A1 PCT/KR2015/010380 KR2015010380W WO2017047856A1 WO 2017047856 A1 WO2017047856 A1 WO 2017047856A1 KR 2015010380 W KR2015010380 W KR 2015010380W WO 2017047856 A1 WO2017047856 A1 WO 2017047856A1
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WO
WIPO (PCT)
Prior art keywords
coating layer
electrode layer
layer
electrode
biosensor strip
Prior art date
Application number
PCT/KR2015/010380
Other languages
English (en)
Korean (ko)
Inventor
최성숙
Original Assignee
최성숙
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 최성숙 filed Critical 최성숙
Publication of WO2017047856A1 publication Critical patent/WO2017047856A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors

Definitions

  • the present invention relates to a biosensor strip and a method for manufacturing the same, and more particularly, to a biosensor strip and a method for manufacturing the same, which can be subjected to physical analysis through body fluids such as blood.
  • Quantitative or qualitative analysis of analytes present in body fluids is of great importance both chemically and clinically.
  • Representative examples include measuring blood glucose in the blood or measuring cholesterol which is a factor of various adult diseases. Especially, in the case of diabetics, it is very important to check the physical condition by measuring blood glucose periodically.
  • the present invention has been made to solve the above problems, by forming a gold electrode layer on top of the copper electrode layer in the reaction unit reacts with the body fluid of the copper electrode layer biosensor strip which can improve the reliability of the measurement value of the body analysis through the body fluid And to provide a method for the production thereof.
  • the present invention includes a base sheet of an insulating material, and the electrode portion formed on the base sheet, the biosensor strip for performing the diagnosis through the body fluid flowing into the electrode portion, the electrode portion on the upper portion of the base sheet
  • the first coating layer and the second coating layer is preferably formed by coating nickel to increase the adhesion between the copper electrode layer and the gold electrode layer.
  • the first coating layer, the second coating layer, the copper electrode layer, and the gold electrode layer are formed by vapor deposition, and the copper electrode layer includes a reference electrode, a working electrode, and a counter electrode.
  • the method of manufacturing a biosensor strip according to the present invention includes coating a first coating layer on an insulating base sheet, and depositing copper on an upper surface of the first coating layer, one side of which is inserted into a measuring instrument to form a connection part connected to the measurement. Forming a copper electrode layer having a reaction part reacting with the raw sample introduced on the other side; coating a second coating layer on an upper surface of the reaction part of the copper electrode layer; and depositing gold on the upper surface of the second coating layer. Forming a step.
  • first coating layer and the second coating layer is preferably formed by depositing nickel to increase the adhesion between the copper electrode layer and the gold electrode layer.
  • the electrode part includes a copper electrode layer and a gold electrode layer.
  • the gold electrode layer is formed only in a part of the reaction part that reacts with the body fluid, it is possible to provide a low cost and high efficiency biosensor strip. There is an advantage to that.
  • FIG. 1 is a perspective view showing a use example of a biosensor strip according to the present invention.
  • FIG. 2 is an exploded perspective view showing an embodiment of the biosensor strip of the present invention
  • FIG. 3 is a cross-sectional view of the biosensor strip of FIG.
  • FIG. 4 is a flowchart illustrating a method of manufacturing a biosensor strip according to the present invention.
  • first and second may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another.
  • the first component may be referred to as the second component, and similarly, the second component may also be referred to as the first component.
  • the blood glucose level or the cholesterol level is read by reading the measurement value measured by the measuring device 1 after the body fluid such as blood or saliva is buried in the strip after mounting the measuring device 1. To measure the back.
  • the biosensor strip 2 is formed on the base sheet 10, the electrode portion 20 formed on the base sheet 10, and the electrode portion 20, as shown in FIGS. 2 and 3.
  • the cover sheet 40 is formed on the adhesive sheet 30 and the adhesive sheet 30.
  • the base sheet 10 may be applied to the PET film commonly used in biosensors, in this embodiment, the PET film is applied as the base sheet 10, in addition to the thin film formed of a material such as PVC or polycarbonate Members on the strip may be applied.
  • the electrode unit 20 is formed on the base sheet 10.
  • One side of the electrode unit 20 is inserted into the measuring device 1, and the other side of the body fluid is injected to react the body fluid for analysis.
  • the electrode unit 20 includes a first coating layer 21, a copper electrode layer 22, a second coating layer 25, and a gold electrode layer 26.
  • the first coating layer 21 is formed by depositing nickel on the base sheet 10 which is a PET film.
  • the first coating layer 21 is formed so that the copper electrode layer 22 can increase the adhesion to the base sheet 10.
  • the copper electrode layer 22 is formed of copper on the upper surface of the first coating layer 21.
  • the copper electrode layer 22 is formed of a reference electrode, a working electrode, and an auxiliary electrode.
  • the copper electrode layer 22 is inserted into the measuring device 1 and inserted into the measuring device 1.
  • the connecting portion 23 is formed to be connected to the connector (not shown) is formed, the reaction portion 24 for the reaction with the body fluid is formed on the other side.
  • the second coating layer 25 is formed by depositing nickel in the same manner as the first coating layer 21, and is formed on the reaction part 24 for reaction with the body fluid in the upper portion of the copper electrode layer 22.
  • the second coating layer 25 is to increase the bonding force between the gold electrode layer 26 and the copper electrode layer 22 to be described later.
  • Nickel forming the first coating layer 21 and the second coating layer 25 is preferably using a boron type or in-type nickel, it is preferably formed so that the thickness is 0.1 to 10 ⁇ m.
  • pretreatment may be performed before the first coating layer 21 and the second coating layer 25 are formed on the base sheet 10 and the copper electrode layer 22, pretreatment may be performed.
  • the surface treatment may be performed using a platinum catalyst. Surface treatment can be performed.
  • the gold electrode layer 26 is formed of an electrode layer using gold on the first coating layer 21. Since the gold electrode layer 26 is formed on the upper portion of the reaction part 24 for the reaction of the body fluid, the reliability of the measured value can be increased while minimizing the amount of gold used.
  • the gold electrode layer 26 may be formed by electroless gold plating or electrolytic gold plating. When electroless gold plating is used, it is preferable to use PGC (Potassium gold cyanide), and the thickness of the gold electrode layer 26 is 0.03 to It is preferable to form so that it may become 1 micrometer.
  • the biosensor strip 2 of the present embodiment is a sensor for measuring blood glucose, and a reaction enzyme 27 is coated on the gold electrode layer 26 and the second coating layer 25 to measure blood glucose.
  • the reaction enzyme 27 may be a glucose oxidase.
  • blood glucose contained in the blood is oxidized by the reaction enzyme 27, and the reaction enzyme 27 is reduced.
  • the reduced reaction enzyme 27 loses electrons on the surface of the working electrode to which a constant voltage is applied and is oxidized electrochemically again. Since the blood glucose concentration of the injected body fluid is proportional to the current generated in the process of oxidizing the reaction enzyme 27, the blood glucose concentration is measured by measuring the amount of this current.
  • the electrode part 20 of the present invention is formed by sequentially stacking the first coating layer 21, the copper electrode layer 22, the second coating layer 25, and the gold electrode layer 26.
  • the adhesive sheet 30 is attached to the upper portion of the electrode unit 20.
  • the adhesive sheet 30 may be formed of a double-sided tape having a predetermined thickness to bond the electrode 20 and the cover sheet 40 to be described later.
  • the adhesive sheet 30 is cut in a corresponding portion so as not to cover the reaction part 24 in which the copper electrode layer 22 is formed.
  • the cover sheet 40 is attached to the upper surface of the adhesive sheet 30.
  • the cover sheet 40 may protect the electrode unit 20 and may print a trade name or other related information on the upper surface.
  • Injection grooves 50 for injecting body fluids are formed in the spaces between the cover sheets 40.
  • the injection groove 50 sucks body fluid, ie, blood, on the principle of capillary phenomenon, so that body fluid may flow into the reaction unit 24.
  • the method of manufacturing the biosensor strip according to the present invention is as shown in FIG. 4.
  • Nickel is deposited on the base sheet 10 to form a first coating layer 21, and a copper electrode layer 22 is formed on the first coating layer 21.
  • the body fluid is injected to form nickel on the upper surface of the reaction unit 24 where the reaction enzyme 27 is reacted to form the second coating layer 25.
  • the gold electrode layer 26 is formed on the upper surface of the second coating layer 25.
  • the adhesive sheet 30 is formed on the electrode portion 20. Attach the cover sheet 40 to the upper surface of the ().
  • the biosensor strip 2 of the present invention thus formed is mounted so that the connecting portion 23 of the copper electrode layer 22 is inserted into the measuring device 1 as shown in FIG. 1, and then the body fluid is injected through the injection groove 50. Then, the analysis value is measured by the measuring device 1.
  • the biosensor strip according to the present invention described above can increase the reliability of the measured value by depositing nickel and forming copper and gold as electrode layers.
  • the present invention can be applied to the field of blood glucose meter for measuring blood glucose using blood, and the industrial applicability is very high.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

La présente invention concerne une bande de biocapteur permettant de réaliser une analyse corporelle par le biais de fluides corporels tels que le sang, et son procédé de fabrication. La présente invention concerne ainsi une bande de biocapteur comprenant une feuille de base constituée d'un matériau isolant, et une partie électrode formée sur la partie supérieure de la feuille de base, la bande de biocapteur réalisant un diagnostic par le biais du fluide corporel pénétrant dans la partie électrode. La partie électrode comprend : une première couche de revêtement formée sur la partie supérieure de la feuille de base ; une couche d'électrode de cuivre formée sur la partie supérieure de la première couche de revêtement ; une seconde couche de revêtement formée sur la partie supérieure de la première couche d'électrode, et appliquée sur une partie de réaction d'échantillon dans laquelle a lieu une réaction avec un fluide corporel ; et une couche d'électrode en or formée sur la partie supérieure de la seconde couche de revêtement.
PCT/KR2015/010380 2015-09-17 2015-10-01 Bande de biocapteur et son procédé de fabrication WO2017047856A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020150131338A KR20150112911A (ko) 2015-09-17 2015-09-17 바이오센서 스트립 및 이의 제조방법
KR10-2015-0131338 2015-09-17

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WO2017047856A1 true WO2017047856A1 (fr) 2017-03-23

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WO (1) WO2017047856A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101865055B1 (ko) * 2016-09-02 2018-06-08 (주)지엠에스바이오 혈당 측정용 스트립
KR102288223B1 (ko) 2019-09-06 2021-08-10 (주)인트인 체액 검사용 챔버 및 이를 이용하는 체액 검사장치

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100805913B1 (ko) * 2005-12-16 2008-02-21 후앙 엘리스 와이 일회용 전기 화학 센서 스트립의 구조 및 그 제조 방법
KR20080082175A (ko) * 2007-03-07 2008-09-11 장용상 바이오센서
KR20100042399A (ko) * 2008-10-16 2010-04-26 안동대학교 산학협력단 전기화학적 바이오센서의 구조 및 바이오센서를 이용한 측정방법
KR20100084233A (ko) * 2009-01-16 2010-07-26 주식회사 에스디 전기화학적 바이오센서 스트립
KR20140066377A (ko) * 2012-11-23 2014-06-02 삼성전자주식회사 바이오 센서

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100805913B1 (ko) * 2005-12-16 2008-02-21 후앙 엘리스 와이 일회용 전기 화학 센서 스트립의 구조 및 그 제조 방법
KR20080082175A (ko) * 2007-03-07 2008-09-11 장용상 바이오센서
KR20100042399A (ko) * 2008-10-16 2010-04-26 안동대학교 산학협력단 전기화학적 바이오센서의 구조 및 바이오센서를 이용한 측정방법
KR20100084233A (ko) * 2009-01-16 2010-07-26 주식회사 에스디 전기화학적 바이오센서 스트립
KR20140066377A (ko) * 2012-11-23 2014-06-02 삼성전자주식회사 바이오 센서

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