WO2017038835A8 - Method of producing plastid transformants - Google Patents

Method of producing plastid transformants Download PDF

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Publication number
WO2017038835A8
WO2017038835A8 PCT/JP2016/075375 JP2016075375W WO2017038835A8 WO 2017038835 A8 WO2017038835 A8 WO 2017038835A8 JP 2016075375 W JP2016075375 W JP 2016075375W WO 2017038835 A8 WO2017038835 A8 WO 2017038835A8
Authority
WO
WIPO (PCT)
Prior art keywords
genes
plastid transformants
suppressed
concentration
wild type
Prior art date
Application number
PCT/JP2016/075375
Other languages
French (fr)
Other versions
WO2017038835A2 (en
WO2017038835A3 (en
Inventor
Asuka Yamaguchi
Jiro Okuma
Yasuhiro Kondo
Yoshitsugu Hirose
Original Assignee
Honda Motor Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2016054448A external-priority patent/JP6696806B2/en
Application filed by Honda Motor Co., Ltd. filed Critical Honda Motor Co., Ltd.
Priority to US15/510,377 priority Critical patent/US20180216124A1/en
Priority to BR112017005716A priority patent/BR112017005716A2/en
Priority to CN201680002875.2A priority patent/CN107109432B/en
Priority to EP16778901.5A priority patent/EP3180436A2/en
Publication of WO2017038835A2 publication Critical patent/WO2017038835A2/en
Publication of WO2017038835A8 publication Critical patent/WO2017038835A8/en
Publication of WO2017038835A3 publication Critical patent/WO2017038835A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation

Abstract

A method of producing plastid transformants including a process (a) in which genes coding for proteins having a function of neutralizing nitrite toxicity are introduced into plant tissues and a process (b) in which the plant tissues into which the genes are inserted in the process (a) are cultured in a medium containing nitrite nitrogen having a concentration at which growth of wild type plant cells is suppressed or a concentration at which regeneration of wild type plants is suppressed and thus plastid transformants obtained by inserting the genes into plastid genomes are screened.
PCT/JP2016/075375 2015-08-28 2016-08-24 Method of producing plastid transformants WO2017038835A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US15/510,377 US20180216124A1 (en) 2015-08-28 2016-08-24 Method of producing plastid transformants
BR112017005716A BR112017005716A2 (en) 2015-08-28 2016-08-24 method for producing plastid transformants
CN201680002875.2A CN107109432B (en) 2015-08-28 2016-08-24 Method for producing plastid transformants
EP16778901.5A EP3180436A2 (en) 2015-08-28 2016-08-24 Method of producing plastid transformants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2015-169817 2015-08-28
JP2015169817 2015-08-28
JP2016054448A JP6696806B2 (en) 2015-08-28 2016-03-17 Method for producing plastid transformant
JP2016-054448 2016-03-17

Publications (3)

Publication Number Publication Date
WO2017038835A2 WO2017038835A2 (en) 2017-03-09
WO2017038835A8 true WO2017038835A8 (en) 2017-05-11
WO2017038835A3 WO2017038835A3 (en) 2017-06-15

Family

ID=57121472

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2016/075375 WO2017038835A2 (en) 2015-08-28 2016-08-24 Method of producing plastid transformants

Country Status (1)

Country Link
WO (1) WO2017038835A2 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69830317T3 (en) * 1997-08-07 2009-03-19 Auburn University, Auburn UNIVERSAL INTEGRATION AND EXPRESSION VECTORS FOR CHLOROPLASTS, TRANSFORMED PLANTS AND PRODUCTS HEREVON
EP1294221A4 (en) * 2000-03-02 2003-06-25 Univ Auburn Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
CA2491639A1 (en) * 2002-07-03 2004-01-15 University Of Central Florida Expression of the human igf-1 in transgenic plastids

Also Published As

Publication number Publication date
WO2017038835A2 (en) 2017-03-09
WO2017038835A3 (en) 2017-06-15

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