WO2017023070A1 - Composition for promoting fatigue recovery containing, as an active ingredient, extracellular polysaccharides produced by ceriporia lacerate - Google Patents

Abstract

The present invention relates to extracellular polysaccharides produced by Ceriporia lacerate, a mycelial culture broth of Ceriporia lacerate, containing the extracellular polysaccharides, a composition for promoting fatigue recovery containing, as an active ingredient, a dried powder of the mycelial culture broth or an extract of the mycelial culture broth, and a health functional food for promoting fatigue recovery. The composition and health functional food of the present invention have an excellent fatigue recovery effect.

Description

Fatigue recovery enhancing composition containing an extracellular polysaccharide produced by Ceriporia laccerata as an active ingredient

The present invention is an extracellular polysaccharide produced by Ceriporia lacerata ( Ceriporia lacerata ), mycelium culture medium of the seriporia laccerata comprising the extracellular polysaccharide, the dry powder of the mycelium culture medium, or the extract of the mycelium culture solution It relates to a composition for promoting fatigue recovery containing as an active ingredient.

Fatigue is a condition in which work efficiency decreases when working, a state of constant disruption, or a condition in which the cerebral cortex is restrained. 'Can be defined.

The expression of fatigue or stress is a condition in which the mental and physical functions are not smoothly performed, and the fatigue is a state in which the ability to perform muscle contraction activity, that is, exercise performance ability is insufficient. The reduced state (Gibson H, et al, Sports Med . 1985. 2 (2): 120-32), in contrast to physical fatigue, stresses can be interpreted as an imbalance of physical rhythm due to mental overload. Can be. Therefore, fatigue in the broad sense is a concept that includes both fatigue and stress, and can be said to be a reduction in the ability to perform physical or mental activities. Specifically, fatigue is a state in which work efficiency decreases mainly due to physical fatigue, and stress is mental. It refers to a state where confusion of homeostasis occurs due to fatigue (establishment of functional evaluation system related to fatigue recovery of KFDA's health functional food, research report)

In addition, the accumulation of fatigue often leads to chronic fatigue and can lead to many diseases such as peptic ulcer, hypertension, diabetes and the like. Cancer, stroke, and heart disease are the three major causes of death in modern people, and overwork is the main cause of their deaths. Recently, the need for scientific food intake for efficient fatigue recovery is increasing.

On the other hand, Ceriporia laccerata is a type of white fungus, and is known to perform co-metabolism called lignin decomposition in order to use carbon sources such as cellulose, hemicellulose, other polysaccharides, and glycerol in an ecosystem.

Regarding the medical treatment using Ceriporia laccerata, only the use of Ceriporia laccerata culture extract in Korean Patent No. 10-1031605, filed by the present inventors, is known so far. Increasing fatigue recovery with Ceriporia laccerata has not been reported.

Accordingly, the present inventors completed the present invention by confirming that the extracellular polysaccharide isolated from Seriphoria laccerata, the mycelium culture solution of Seriphoria laccerata, dried powder or extract thereof exhibits an effect of promoting fatigue recovery. It was.

SUMMARY OF THE INVENTION An object of the present invention is to provide a composition for promoting fatigue recovery and a health functional food for promoting fatigue recovery, comprising an active ingredient produced by Seriphoria laccerata.

Another object of the present invention is to provide a method for recovering fatigue by administering an active ingredient produced by Ceriporia laccerata, and an active ingredient produced by Ceriporia laccerata for use in the manufacture of a medicament for fatigue recovery. It is to provide a use.

In order to achieve the above object, the present invention is an extracellular polysaccharide produced by Ceriporia lacerata ( Ceriporia lacerata ), mycelium culture medium of the seriporia laccerata comprising the extracellular polysaccharide, the seriporia lacsera It provides a dry powder of another mycelium culture solution, or an extract of the mycelium culture solution of the seriporia laccerata as an active ingredient, a composition for promoting fatigue recovery and health functional food.

In addition, the present invention provides a method for the fatigue recovery of administering the active ingredient produced by Ceriporia laccerata, and the active ingredient produced by Ceriporia laccerata for use in the manufacture of a medicament for fatigue recovery. Provides use.

Composition and health functional food containing the active ingredient produced by Ceriporia lacerata ( Ceriporia lacerata ) according to the present invention as an active ingredient has excellent fatigue recovery effect.

1 is a schematic diagram of a Matsumoto swimming pool using a forced swimming device of Example 2. FIG.

Figure 2 is a graph showing the forced swimming time of ICR mice administered with various concentrations of the extracellular polysaccharide produced by Seriphoria lacserata.

Figure 3 is a graph showing the LDH concentration in serum after forced swimming of ICR mice administered with various concentrations of the extracellular polysaccharide produced by Seriphoria lacserata.

The present invention is an extracellular polysaccharide produced by Ceriporia lacerata ( Ceriporia lacerata ), mycelium culture medium of the seriporia laccerata containing the extracellular polysaccharide, dry powder of the mycelium culture medium or extract of the mycelium culture solution It provides a composition for promoting fatigue recovery, containing as an active ingredient.

As used herein, the term "extracellular polysaccharide (EPS)" is a part of the cell wall of a microorganism such as a fungus, and the polysaccharide is secreted to the outside of the cell to form a capillary around it or secreted into the periphery or medium as a slime. Means. The extracellular polysaccharide is secreted by the microorganism to protect itself from the external environment such as antibodies, toxic substances, protozoa and bacteriophage.

The extracellular polysaccharide is 40 to 60% by weight of sugar and 30 to 40% by weight of protein, 40 to 50% by weight of sugar and 32 to 38% by weight of protein, or 43 to 47% by weight of sugar and 33 to 36% by weight. % Protein, and more specifically about 45% sugar and about 34% protein.

The sugar may contain mannose, galactose and glucose.

The extracellular polysaccharide may have a molecular weight of 100 ~ 150 kDa, 110 ~ 140 kDa or 115 ~ 125 kDa, more specifically may have a molecular weight of about 120 kDa.

In one embodiment of the present invention, the extracellular polysaccharide comprises the steps of: (a) preparing a mycelium culture medium of the seriporia laccerata by liquid culture of the seriporia laccerata mycelium; (b) drying the powdered mycelium culture medium of the seriporia laccerata; And (c) extracting the dry powder of the mycelium culture medium of Seriphoria laccerata with a solvent, filtering the same, and concentrating under reduced pressure.

The medium for the liquid culture of the seriporia laccerata mycelium in the step (a) is sugar, glucose, starch, hydrate, soybean meal, soybean meal, magnesium sulfate (MgSO ₄ ), potassium phosphate (KH ₂ PO ₄ ), potassium diphosphate (K ₂ HPO ₄ ) and may include water, the hydrogen ion concentration may be pH 4.5 ~ 6.0.

Specifically, the medium, 0.2 to 3% by weight of sugar, 0.2 to 3% by weight of glucose, 0.2 to 4% by weight, 0.1 to 0.5% by weight of water, 0.1 to 0.5% by weight of wheat flour, 0.2 to 3 weight of soy flour %, 0.05% to 0.1% by weight of magnesium sulfate (MgSO ₄ ), 0.05% to 0.25% by weight of potassium monophosphate (KH ₂ PO ₄ ), 0.05% to 0.25% by weight of potassium diphosphate (K ₂ HPO ₄ ) and the balance of water Can be.

The liquid culture in the step (a) is carried out under a blue LED light source, it can be carried out by maintaining the concentration of carbon dioxide at 1,000 ~ 2,000 ppm.

The liquid culture, for example, at 20 ~ 28 ℃, hydrogen ion concentration (pH) 4.5 ~ 6.0, the light source maintains a blue LED, the illuminance is 0.1 ~ 0.8 LUX and air is injected at 0.5 ~ 2.0 kgf / ㎠ and carbon dioxide The concentration of may be performed for 8 to 13 days while maintaining at 1,000 to 2,000 ppm. Specifically, it may be performed for 5 to 15 days at the conditions of 20 ~ 25 ℃, pH 4.5 ~ 6.0, 0.5 ~ 2.0 kgf / ㎠, carbon dioxide concentration of 1,000 ~ 2,000 ppm. When the liquid culture under the conditions described above, the content of the extracellular polysaccharide produced is high.

As the parent strain in step (a), one excellent strain stored at 1 to 5 ° C. in PDA (Potato dextrose agar) medium was used in a shaker incubator using PDB (Potato dextrose broth) medium in an Erlenmeyer flask. Maintain a constant temperature of 25 ℃ can be used after 7 to 9 days incubation process. In addition, after culturing the parent strains, the culture solution or the obtained mycelium can be used as the inoculum. Specifically, the amount of mycelium to be added to the inoculum may be about 0.5% (w / v) based on the amount of the solution to be cultured. The amount of extracellular polysaccharides does not increase as the amount of mycelia (% / 100 ml, w / v) increases, so the medium composition has the highest content of extracellular polysaccharides, not the best nutritional ratio and environmental conditions for the growth of mycelia. It is preferable to apply the selective culture conditions to form.

The culture solution can be separated and purified into a mycelium and an aqueous solution. For the separation and purification, the solution from which the mycelium is removed by centrifugation may be repeatedly purified using a multi-sheet filter press and a vibration centrifugal separator (PALLSEP), and then irradiated with ultraviolet (UV) light for 1 minute. In addition, the culture solution may be stored after sealing to remove oxygen, which may bring a change in the content of the active ingredient due to the growth of the mycelia when the mycelia exist in the culture solution.

In step (b), the mycelia culture solution prepared in step (a) may be dried and powdered. The drying may be performed for 48 to 96 hours at a temperature of 40 ℃ or less, more specifically 30 ℃ or less to prevent the disappearance of the active material. In addition, the drying of step (b) is preferable to use a vacuum freeze dryer rather than a vacuum dryer that sets the evaporation temperature relatively high because the change in the effective substance content is minimized.

In the step (c), after extracting the dry powder of the mycelia culture solution obtained in step (b) with a solvent, the extracellular polysaccharide which is the active ingredient of the composition according to the present invention is separated.

Specifically, 100 ml of distilled water is added to the dry powder of 3 to 10 g of the mycelium culture medium, and then suspended well, followed by centrifugation at 5,000 to 10,000 rpm for 10 to 30 minutes to obtain a supernatant. An extraction solvent corresponding to a pear may be added and placed in a refrigerator at 1 to 5 ° C. for 10 to 15 hours. After only centrifuging the supernatant again at 5,000 to 10,000 rpm for 10 to 30 minutes in the stationary material, the precipitate may be recovered to prepare a crude extracellular polysaccharide. The crude extracellular polysaccharide can be vacuum-dried at 30 ° C. or lower to obtain extracellular polysaccharide.

The extraction solvent may be a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, acetone, ether, chloroform and ethyl acetate or a mixed solvent thereof, and more specifically, water, methanol, ethanol, butanol It may be a solvent selected from the group consisting of acetone and ethyl acetate or a mixed solvent thereof, and more specifically, may be water or 50 to 80% (v / v) ethanol aqueous solution.

The composition for promoting fatigue recovery of the present invention not only provides excellent fatigue recovery enhancing effect but also has little toxicity and side effects due to the drug, so that it can be used with confidence even during long-term use. The fatigue recovery promoting effect means the action of preventing or inhibiting the symptoms resulting from the increase in lactate dehydrogenase (LDH). According to one embodiment of the present invention, the composition for promoting fatigue recovery of the present invention showed a fatigue recovery enhancing effect by reducing the LDH concentration of the administration group.

The composition for promoting fatigue recovery according to the present invention may further include appropriate carriers, excipients and diluents commonly used.

The fatigue recovery enhancing composition may include 0.1 to 80% by weight, specifically, 0.1 to 50% by weight of the extracellular polysaccharides relative to the total weight of the composition. In addition, the composition for promoting fatigue recovery may be appropriately included in the amount corresponding to the content of the extracellular polysaccharide, the mycelium culture medium, dry powder thereof or the extract of the mycelium culture medium of the three liporia laccerata. However, the effective amount of the extracellular polysaccharide, the culture solution containing the same, the dry powder of the culture solution or the extract of the culture solution may be appropriately adjusted according to the method of use and purpose of the composition.

The compositions according to the invention can each be formulated and used according to conventional methods. Suitable formulations include, but are not limited to, tablets, pills, powders, granules, dragees, hard or soft capsules, solutions, suspensions or emulsions, injections, suppositories, and the like.

The composition according to the invention can be prepared in a suitable formulation using a pharmaceutically inert organic or inorganic carrier. That is, when the formulation is a tablet, coated tablet, dragee and hard capsule, lactose, sucrose, starch or derivatives thereof, talc, calcium carbonate, gelatin, stearic acid or salts thereof can be used. In addition, when the formulation is a soft capsule, polyols of vegetable oils, waxes, fats, semisolids and liquids can be used. In addition, when the formulation is in the form of a solution or syrup, water, polyols, glycerol, vegetable oils and the like can be used.

The composition according to the present invention may further include a preservative, a stabilizer, a wetting agent, an emulsifier, a solubilizer, a sweetener, a colorant, an osmotic agent, an antioxidant, and the like in addition to the above carrier.

The method of administering the composition according to the present invention can be easily selected according to the dosage form, and can be administered orally or parenterally. The dosage may vary depending on the patient's age, sex, weight, severity, and route of administration, but is generally 5 to 500 mg / kg body weight, specifically 100 to 250 based on the extracellular polysaccharide as an active ingredient. The amount of mg / kg body weight may be administered once to three times a day. However, the dosage does not limit the scope of the invention in any aspect.

In addition, the present invention is an extracellular polysaccharide produced by Ceriporia lacerata ( Ceriporia lacerata ), mycelium culture medium of the seriporia laccerata comprising the same, the dry powder of the mycelium culture solution or the extract of the mycelium culture solution active ingredient Including, to provide a functional dietary supplement for fatigue recovery.

The extracellular polysaccharide produced by the Seriphoria laccerata, the mycelium culture solution of the Seriporia laccerata, the dry powder of the mycelium culture solution or the extract of the mycelium culture medium are as described above.

The health functional food according to the present invention may be in the form of powder, granules, tablets, capsules or beverages, and may be candy, chocolate, beverages, gums, teas, vitamin complexes or dietary supplements.

In this case, the content of the extracellular polysaccharide according to the present invention contained in the health functional food, mycelium culture medium containing the same, the dry powder of the mycelium culture medium or the extract of the mycelium culture medium, usually 0.01 to 50% by weight of the total food weight Specifically, it may be included in 0.1 to 20% by weight. In addition, when the health functional food is a beverage, based on 100 ml of the beverage, extracellular polysaccharide according to the present invention, mycelium culture medium containing the same, dry powder of the mycelium culture medium or extract of the mycelium culture medium, 0.02 to 10 g For example, the content may be included in an amount of 0.3 to 1 g.

The health functional food further comprises a food supplement acceptable with the extracellular polysaccharide of the present invention, a mycelium culture medium of the seriporia laccerata containing the same, a dry powder of the mycelium culture medium or an extract of the mycelium culture medium. Can be.

The present invention is an extracellular polysaccharide produced by Ceriporia lacerata ( Ceriporia lacerata ), mycelium culture medium of the seriporia laccerata comprising the extracellular polysaccharide, the dry powder of the mycelium culture medium, or the extract of the mycelium culture solution It provides a method for fatigue recovery, comprising administering to a subject in need of fatigue recovery.

The subject may be a mammalian animal, more specifically a human.

Furthermore, the present invention is an extracellular polysaccharide produced by Ceriporia lacerata for use in the manufacture of a medicament for fatigue recovery, mycelium culture medium of the Ceriporia laccerata comprising the extracellular polysaccharide. It provides a dry powder of the mycelium broth, or the use of the extract of the mycelium broth.

The extracellular polysaccharide produced by the Seriphoria laccerata, the mycelium culture medium of the Seriphoria laccerata containing the extracellular polysaccharide, the dry powder of the mycelium culture solution, or the extract of the mycelium culture medium is as described above.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

[ Example ]

Production Example  One. Serifolia Lacserata  Mycelium broth, its dry powder, extract and Extracellular polysaccharide (exopolysaccharide; hereinafter referred to as "EPS")

1-1: Serifolia Lacserata  Preparation of Mycelia Culture

The seedlings grown in subcultured oak reimbursement in Sangju, Gyeongbuk were grown by subculture and stored frozen at -80 ℃. The cryopreserved strain was passaged 2-3 times in PDA (Potato dextrose agar) medium (87 plastic cultures; Difco, Becton Dickinson and Company) and stored the strain (hereinafter referred to as “PDA culture strain”) in a 4 ° C. refrigerator. Was used. Then, after preparing 600 ml of PDB (Potato dextrose broth) medium (Difco, Becton Dickinson and Company) in an Erlenmeyer flask, one PDA culture strain was added thereto, followed by shaking culture at 25 ° C. for 8 days to obtain a PDB culture strain.

On the other hand, 1.5% by weight of sugar, 0.5% by weight of glucose, 0.5% by weight of potato starch, 0.25% by weight of water, 0.25% by weight of soybean meal, 0.75% by weight of soy flour, 0.05% by weight of magnesium sulfate (MgSO ₄ ) , Liquid culture medium containing 0.05% by weight of potassium monophosphate (KH ₂ PO ₄ ), 0.05% by weight of potassium diphosphate (K ₂ HPO ₄ ) and the balance of water at 121 ° C. in an 800 L fermenter and air at 1.5 kgf / Injected in cm 2 sterilized for 20 minutes. Thereafter, 600 ml of the PDB culture strain was inoculated with a starter while cooled to 23 ° C., while the air was aerated at 0.5 to 1.5 kgf / cm 2, the light source maintained the blue LED, the illuminance was 0.5 LUX, and the concentration of carbon dioxide was The mycelium culture of Ceriporia laccerata was prepared by liquid culture of the Ceriporia laccerata mycelium at 2,000 ppm for 10 days at a constant temperature of 23 ° C.

1-2: Serifolia Lacserata  Preparation of dry powder of mycelium culture

The dry powder of the mycelium culture medium of Seriphoria laccerata was prepared by lyophilizing the culture medium of the culture medium of Liporice laccerata prepared in Preparation Example 1-1 by lyophilizing at 25 ° C. for 72 hours.

1-3: Serifolia Lacserata  Preparation of extract of mycelium culture

100 ml of distilled water was added to 5 g of the dry powder of the mycelium culture medium of Ceriporia laccerata prepared in Preparation Example 1-2, and then suspended well, and centrifuged at 8,000 rpm for 20 minutes. Ethanol corresponding to 2-3 times the volume of the supernatant was added to the supernatant, and the mixture was allowed to stand at 4 ° C. for 12 hours. Then, the supernatant was taken from the stationary material to prepare an extract of the mycelium culture medium of Seriporia laccerata.

1-4 Serifonia Lacserata  Preparation of EPS from Mycelial Culture

The extract of the mycelium culture medium of Seriphoria laccerata prepared in Preparation Example 1-3 was further centrifuged at 8,000 rpm for 20 minutes, and the precipitate was recovered to obtain a crude EPS. The crude EPS was vacuum-dried at 25 ° C. for 72 hours using a vacuum freeze dryer to obtain EPS produced by Ceriporia laccerata.

Example  1. Characterization of EPS

1-1: Gel permeation  Chromatography (Gel Permeation Chromatography, GPC Molecular weight of EPS using

The EPS prepared in Preparation Example 1 was dissolved in 0.1 M Na ₂ SO ₄ /0.05 M NaN ₃ [adjusting pH to 4 with glacial acetic acid] to 1% (w / v) in a solution, and then at 4,000 rpm. After centrifugation for 0.5 hour, only the supernatant was filtered with a 0.45 μm syringe filter and analyzed by GPC.

For the GPC analysis, the refractive index was used as the detector, and the GPC column was adjusted to pH 4 with 0.1 M Na ₂ SO ₄ /0.05 M NaN ₃ [glacial acetic acid using OHpak SB 805 HQ (Shodex, Japan). ], The flow rate of the mobile phase was flowed at a rate of 1.0 mL / min. Standard curves were prepared using Dextran (American Polymer Corporation, USA) with different molecular weights (130 kDa, 400 kDa, 770 kDa, or 1200 kDa), and Knauer K-2310 (refractive index (RI) measuring instrument). Germany) to determine the molecular weight of EPS. The measurement conditions are summarized in Table 1 below.

	Measurement of molecular weight
GPC system	Knauer K-501 System
column	OHpak SB 805 HQ (Shodex, Japan)
Mobile phase	0.1 M Na 2 SO 4 / 0.05 M NaN 3 / pH 4
Flow rate	1.0 ml / min
Measuring instrument	RI (Knauer K-2310)

As a result, the molecular weight of EPS of the present invention was found to be about 120 kDa.

1-2: Determination of sugar and protein content of EPS

The EPS prepared in Preparation Example 1 was second-purified and treated with proteolytic enzymes to determine sugar and protein content.

Specifically, the super purified EPS (EPS prepared in Preparation Example 1) was dissolved in distilled water and centrifuged at 8,000 rpm for 20 minutes to separate the supernatant. Ethanol corresponding to 2-3 times the volume of the supernatant was added to the separated supernatant and placed in a 4 ° C. refrigerator for 12 hours. Thereafter, only the supernatant was again centrifuged at 8,000 rpm for 20 minutes, and the precipitate was recovered to obtain a second purified EPS. The second purified EPS was dissolved in distilled water, and the proteolytic enzyme alcalase was treated at 50 ° C. for 30 minutes at a concentration of 0.5% (w / v).

Sugar content was measured by the phenol-sulfuric acid method. Specifically, 25 μl of 80% (w / v) phenol was added to 1 mL of the sample diluted by concentration, and then 2.5 mL of sulfuric acid was added and cooled to room temperature. The absorbance was measured at 465 nm to calculate the sugar content.

In addition, the protein content was measured by the BCA method (Smith PK et al., Analytical Biochemistry , 150 (1): 76-85, 1985), and bovine serum albumin was used as a standard.

The sugar and protein content measured as described above are shown in Table 2 below, wherein the EPS content was found to be 45-51 wt% and the protein content was 33-34 wt%.

	yield(%)	Total sugar content (%)	Total protein content (%)
EPS	1.22 ± 0.03	45.32 ± 1.41	34.17 ± 0.73
Secondary Refined EPS	0.78 ± 0.01	50.49 ± 0.52	33.50 ± 2.79
Enzyme-treated EPS *	0.24 ± 0.06	51.39 ± 1.32	34.61 ± 1.51

^* Enzyme treatment: 0.5% Al Calle Izu, 50 ℃, 30 minutes.

Each figure is mean ± SE (n≥3).

In addition, the results of the sugar composition analysis of EPS, EPS mainly contained mannose, galactose and glucose.

Example  2. Measurement of EPS's fatigue recovery enhancement effect

In order to confirm the fatigue recovery enhancement effect of EPS of Preparation Example 1, the test animals administered EPS were subjected to a forced swimming test, and the fatigue recovery enhancement effect was evaluated to increase the swimming time.

Specifically, 8-week-old ICR mice were prepared by dispersing EPS of Preparation Example 1 in drinking water and administered to experimental animals for 1 week using zonation. The dose was 100 mg / kg body weight, 250 mg / kg body weight or 500 mg / kg body weight once daily. For comparison, in the positive control group, red ginseng concentrate (manufacturer: Jeong Kwan Jang, product name: red ginseng tablet plus) was administered 500 mg / kg body weight, and in the negative control group, drinking water was administered. The forced swimming device used the Matsumoto swimming pool shown in Fig. 1 and the flow rate was adjusted to 30 L / min.

On the day of the test (after 3 and 7 days after the start of the experiment), the experimental animals were dosed with EPS (experimental group), red ginseng concentrate (positive control group) or drinking water (negative control group), and the longest swimming time was measured after 60 minutes. A weight of about 5% of the weight of the test animals was attached to the tail, and the longest swimming time was set to the point where the head of the test animal was submerged for 5 seconds or more under water. On the seventh day of the experiment, the longest swimming time was measured repeatedly with a rest time of 60 minutes after the end of swimming, and the measured longest swimming time is shown in FIG. 2. In addition, LDH levels in serum, which is a fatigue-inducing substance after the 7th day of forced swimming, are shown in FIG.

As shown in FIG. 2, the swimming time was significantly increased in the experimental group administered 100 mg / kg body weight or 250 mg / kg body weight of the EPS of the present invention when compared to the negative control group and the positive control group during the forced swimming on the third day of the experiment. It was. In addition, the swimming time was increased in the experimental group administered the EPS of the present invention during the forced swimming on the 7th day of the experiment.

As shown in FIG. 3, compared to the negative control group, LDH in serum, a fatigue-inducing substance, was reduced in all the experimental groups to which the EPS of the present invention was administered.

Claims (19)

1. Extracellular polysaccharide produced by Ceriporia lacerata ( Ceriporia lacerata ), mycelium culture medium of the seriporia laccerata containing the extracellular polysaccharide, dry powder of the mycelium culture solution or extract of the mycelium culture medium as an active ingredient A composition for promoting fatigue recovery containing.
2. The method of claim 1,

   The extracellular polysaccharide comprises 40 to 60% by weight of sugar and 30 to 40% by weight of protein, having a molecular weight of 100 to 150 kDa, fatigue recovery composition.
3. The method of claim 2,

   The extracellular polysaccharide comprises 43 to 47% by weight of sugar and 33 to 36% by weight of protein, and has a molecular weight of 115 to 125 kDa, fatigue recovery composition.
4. The method of claim 2,

   The sugar contains mannose, galactose and glucose, fatigue recovery composition.
5. The method of claim 1,

   The extracellular polysaccharide,

   (a) liquid culture of the seriporia laccerata mycelium to prepare a mycelium culture medium of the seriporia laccerata;

   (b) drying the powdered mycelium culture medium of the seriporia laccerata; And

   (c) extracting the dry powder of the mycelium culture medium of the seriporia laccerata with a solvent and then filtering and concentrating under reduced pressure, a composition for improving fatigue recovery.
6. The method of claim 5,

   The medium for culturing the liquid is sugar, glucose, starch, hydrated water, soybean meal, soybean meal, magnesium sulfate (MgSO ₄ ), potassium monophosphate (KH ₂ PO ₄ ), potassium diphosphate (K ₂ HPO ₄ ) and water To include, the hydrogen ion concentration is pH 4.5 ~ 6.0, fatigue recovery composition.
7. The method of claim 5,

   The liquid culture is carried out under a blue LED light source, is carried out by maintaining the concentration of carbon dioxide at 1,000 ~ 2,000 ppm, fatigue recovery composition.
8. The method of claim 1,

   The extracellular polysaccharide is included in an amount of 0.1 to 80% by weight relative to the total weight of the composition, fatigue recovery composition.
9. Fatigue comprising extracellular polysaccharide produced by Ceriporia lacerata , mycelium culture solution of Ceriporia laccerata , dried powder of the mycelium culture solution or extract of the mycelium culture solution as an active ingredient. Health functional foods to promote recovery.
10. The method of claim 9,

    The extracellular polysaccharide comprises 40 to 60% by weight of sugar and 30 to 40% by weight of protein, and has a molecular weight of 100 to 150 kDa, health functional food for fatigue recovery.
11. The method of claim 10,

    The extracellular polysaccharide comprises 43 to 47% by weight of sugar and 33 to 36% by weight of protein, and has a molecular weight of 115 to 125 kDa, health functional food for promoting fatigue recovery.
12. The method of claim 10,

    The sugar containing mannose, galactose and glucose, health functional food for promoting fatigue recovery.
13. The method of claim 9,

    The extracellular polysaccharide,

    (a) liquid culture of the seriporia laccerata mycelium to prepare a mycelium culture medium of the seriporia laccerata;

    (b) drying the powdered mycelium culture medium of the seriporia laccerata; And

    (c) extracting the dry powder of the mycelium culture medium of Seriphoria laccerata with a solvent and then filtering and concentrating under reduced pressure, a health functional food for improving fatigue recovery.
14. The method of claim 13,

    The medium for culturing the liquid is sugar, glucose, starch, hydrated water, soybean meal, soybean meal, magnesium sulfate (MgSO ₄ ), potassium monophosphate (KH ₂ PO ₄ ), potassium diphosphate (K ₂ HPO ₄ ) and water To include, the hydrogen ion concentration is pH 4.5 ~ 6.0, health functional food for fatigue recovery enhancement.
15. The method of claim 13,

    The liquid culture is carried out under a blue LED light source, the carbon dioxide is maintained by maintaining the concentration of 1,000 to 2,000 ppm, the health functional food for promoting fatigue recovery.
16. The method of claim 9,

    The health functional food is in the form of a powder, granules, tablets, capsules or beverages, fatigue functional health functional food.
17. The method of claim 9,

    The health functional food is candy, chocolate, beverages, gum, tea, vitamin complexes or health supplement foods, fatigue functional health functional foods.
18. Fatigue recovery of the extracellular polysaccharide produced by Ceriporia lacerata , the mycelium culture solution of the Ceriporia laccerata containing the extracellular polysaccharide, the dry powder of the mycelium culture solution, or the extract of the mycelium culture solution A fatigue recovery method comprising administering to a subject in need thereof.
19. Extracellular polysaccharide produced by Ceriporia lacerata , mycelium culture medium of Ceriporia laccerata comprising the extracellular polysaccharide, for use in the preparation of a medicament for fatigue recovery, Use of dry powder or extract of the mycelium culture.

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