WO2016179417A3 - Exosome delivery of micrornas - Google Patents

Exosome delivery of micrornas Download PDF

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Publication number
WO2016179417A3
WO2016179417A3 PCT/US2016/031031 US2016031031W WO2016179417A3 WO 2016179417 A3 WO2016179417 A3 WO 2016179417A3 US 2016031031 W US2016031031 W US 2016031031W WO 2016179417 A3 WO2016179417 A3 WO 2016179417A3
Authority
WO
WIPO (PCT)
Prior art keywords
micrornas
mirna
exosome delivery
subject
mrna
Prior art date
Application number
PCT/US2016/031031
Other languages
French (fr)
Other versions
WO2016179417A2 (en
Inventor
Ryan O'connell
Margaret ALEXANDER
Original Assignee
The University Of Utah Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Utah Research Foundation filed Critical The University Of Utah Research Foundation
Publication of WO2016179417A2 publication Critical patent/WO2016179417A2/en
Publication of WO2016179417A3 publication Critical patent/WO2016179417A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific

Abstract

A method of repressing a target messenger RNA (mRNA) in a subject is provided. The method may include the step of loading exosomes with a microRNA (miRNA), wherein the miRNA is configured to repress the target mRNA. The method may further include the step of introducing the one or more miRNA-loaded exosomes into the subject.
PCT/US2016/031031 2015-05-06 2016-05-05 Exosome delivery of micrornas WO2016179417A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562157875P 2015-05-06 2015-05-06
US62/157,875 2015-05-06

Publications (2)

Publication Number Publication Date
WO2016179417A2 WO2016179417A2 (en) 2016-11-10
WO2016179417A3 true WO2016179417A3 (en) 2016-12-15

Family

ID=57218371

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/031031 WO2016179417A2 (en) 2015-05-06 2016-05-05 Exosome delivery of micrornas

Country Status (1)

Country Link
WO (1) WO2016179417A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020023767A1 (en) * 2018-07-26 2020-01-30 Joslin Diabetes Center Targeting micro-rnas for exosomal delivery or cellular retention
BR112021007287A2 (en) * 2018-10-19 2021-07-27 Ohio State Innovation Foundation extracellular vesicles for targeted therapies against myeloid-derived suppressor cells
CN109750068A (en) * 2019-01-29 2019-05-14 兰州大学 A method of so that excretion body is carried external source microRNA
KR20200113556A (en) * 2019-03-25 2020-10-07 황인후 Composition for preventing or treating diabetes and method for screening antidiabetic agents using ST8SIA1

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009100955A1 (en) * 2008-02-14 2009-08-20 Istituto Superiore di Sanità Antisense rna targeting cxcr4
US20090297498A1 (en) * 2005-05-13 2009-12-03 Ping Wang Milk Fat Globule Epidermal Growth Factor-Factor VIII And Sepsis
US20130053426A1 (en) * 2009-04-17 2013-02-28 Yiqi Seow Composition For Delivery Of Genetic Material
WO2013090523A2 (en) * 2011-12-13 2013-06-20 Henry Ford Health System Methods, systems, and compositions for cell-derived/vesicle-based microrna delivery

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090297498A1 (en) * 2005-05-13 2009-12-03 Ping Wang Milk Fat Globule Epidermal Growth Factor-Factor VIII And Sepsis
WO2009100955A1 (en) * 2008-02-14 2009-08-20 Istituto Superiore di Sanità Antisense rna targeting cxcr4
US20130053426A1 (en) * 2009-04-17 2013-02-28 Yiqi Seow Composition For Delivery Of Genetic Material
WO2013090523A2 (en) * 2011-12-13 2013-06-20 Henry Ford Health System Methods, systems, and compositions for cell-derived/vesicle-based microrna delivery

Also Published As

Publication number Publication date
WO2016179417A2 (en) 2016-11-10

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