WO2016157027A1 - Appareil et procédé pour une séparation par le biais de nanoparticules magnétiques - Google Patents

Appareil et procédé pour une séparation par le biais de nanoparticules magnétiques Download PDF

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Publication number
WO2016157027A1
WO2016157027A1 PCT/IB2016/051605 IB2016051605W WO2016157027A1 WO 2016157027 A1 WO2016157027 A1 WO 2016157027A1 IB 2016051605 W IB2016051605 W IB 2016051605W WO 2016157027 A1 WO2016157027 A1 WO 2016157027A1
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WO
WIPO (PCT)
Prior art keywords
magnetic
substance
nanoparticles
complexes
module
Prior art date
Application number
PCT/IB2016/051605
Other languages
English (en)
Inventor
Luca BETTINSOLI
Mauro BRAGA
Riccardo BRAGA
Andrea Gatti
Massimiliano Magro
Fabio Vianello
Original Assignee
Bettinsoli Luca
Braga Mauro
Braga Riccardo
Andrea Gatti
Massimiliano Magro
Fabio Vianello
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bettinsoli Luca, Braga Mauro, Braga Riccardo, Andrea Gatti, Massimiliano Magro, Fabio Vianello filed Critical Bettinsoli Luca
Priority to CN201680020361.XA priority Critical patent/CN107635666B/zh
Publication of WO2016157027A1 publication Critical patent/WO2016157027A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/0335—Component parts; Auxiliary operations characterised by the magnetic circuit using coils
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/30—Combinations with other devices, not otherwise provided for
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Definitions

  • This invention relates to an apparatus and a method for separating at least one target substance from a liquid ' phase .
  • a constant problem in production and industrial purification plants relates to the low cost separation of specific substances dissolved, suspended or dispersed in a solvent. These substances can be pollutants or have an economic value that is sufficiently high to justify their isolation from the related solution, suspension or dispersion, and subsequent recovery.
  • This invention relates to the preceding context, proposing to provide a method and an apparatus based on the use of stable colloidal suspensions of magnetic nanoparticles, able to overcome the aforesaid drawbacks, and in particular to provide a system capable of making possible the separation of a variety of target substances in a simple and repeatable way and at an extremely low cost.
  • Figures la, lb and lc respectively show a perspective view, a top view and a front view of an apparatus covered by this invention, according to a possible embodiment
  • Figures 2a, 2b and 2c respectively show a perspective view, a top view and a front view of a separation module according to a possible embodiment
  • Figure 3 is a diagram of the process according to a variant of this invention.
  • reference number 1 identifies, in its entirety, an apparatus for separating at least one target substance from a liquid phase .
  • the target substance may be solubilised, dispersed or suspended. in such phase.
  • the target substance could comprise or consist of one or more substances selected from curcumin, avidin, xanthine oxidase, rhodamine isothiocyanate, polyphenols, enzymes, peptides, proteins, DNA, RNA, oligonucleotides, phosphates, chromates, arsenites, arsenates, citrinin, oxytetracyline, tannic acid, pioverdine, chlorine, or mixtures thereof.
  • the target substance could comprise or consist of lactoferrin, optionally in combination with one or more substances selected among those mentioned above.
  • this apparatus is modular, which is to say, expandable at will, or as needed, by selecting a suitable number of the modules described below.
  • the apparatus 1 comprises at least a module 2 for mixing at least one target substance and a stable colloidal liquid suspension of magnetic nanoparticles to obtain substance-nanoparticle complexes.
  • the mixing module 2 not only the target substance and the nanoparticles are mixed homogeneously with each other, but in such module 2 the necessary conditions (for example the temperature, pressure, composition and saline concentration and/or nature of the solvent) are also created for the formation of these complexes, which in turn remain in a stable colloidal liquid suspension.
  • the necessary conditions for example the temperature, pressure, composition and saline concentration and/or nature of the solvent
  • the mixing module 2 could be temperature-controlled to promote formation of the substance-nanoparticle complexes.
  • the mixing module 2 delimits an internal compartment for receiving the target substance and the colloidal suspension of magnetic nanoparticles , in which a mechanical mixing member could be inserted in a rotatable manner.
  • the mixing module 2 could be fed through supply tanks 78, 80 respectively of the target substance and the liquid colloidal suspension of magnetic nanoparticles (for example in water) .
  • the target substance could be pre-mixed in a further liquid, so that liquid and substance can be supplied through a single tank.
  • the magnetic , nanoparticles comprise or consist of superparamagnetic nanoparticles of stoichiometrically pure maghemite.
  • the magnetic nanoparticles usable in this invention could be manufactured according to the procedure discussed in document WO2012010200A1.
  • the magnetic nanoparticles used should be able to create stable colloidal suspensions in aqueous phase for at least six months, without the need of a coating, by means of treatment in an ultrasonic bath.
  • the concentration of magnetic nanoparticles in colloidal suspension in the liquid phase is preferably about 100-1000 mg/l, optionally 200-800 mg/l, advantageously 400-600 mg/l, preferably about 500 mg/l.
  • the magnetic nanoparticles are free of a superficial functionalisation .
  • the magnetic nanoparticles are functionalised, directly or indirectly, in order to bind the target substance.
  • the possible functionalisation (or plurality of functionalisations ) may be carried out in aqueous phase and ensure the colloidal stability of the suspension of magnetic nanoparticles.
  • the size distribution of the magnetic nanoparticles could be in the range 5-50 nm (for example 10-12 nm) , and/or the polydispersity index of the magnetic nanoparticles could be in the range 1.02 to 1.05.
  • Apparatus 1 comprises at least a first magnetic separation module 4 comprising a separation chamber 6, in fluidic communication with the mixing module 2 to receive therefrom a stable colloidal liquid suspension of the substance-nanoparticle complexes, and at least a first magnetic element 8 functionally connected to the separation chamber 6 to magnetically attract said complexes .
  • the substance-nanoparticle complexes remain anchored to the element by virtue of the magnetic properties of the nanoparticles, separating from the colloidal suspension and concentrating the complexes in the region adjacent to said magnetic element, while the liquid surrounding the complexes will not undergo any magnetic influence of relevance.
  • the first magnetic element 8 could comprise a magnetised plate 74, preferably arranged below the separation chamber 6.
  • the plate 74 may be at least partially made of iron, advantageously steel, preferably FE360.
  • a plurality of permanent magnets could be placed in correspondence of the magnetised plate 74 (for example, placed at least partly inside it) in order to generate a substantially homogeneous magnetic field in said chamber 6.
  • any feature referring to the first magnetic separation module 4 could be equally applied also to the second magnetic separation module 14, described below.
  • these modules could function and be shaped in a substantially corresponding or identical way.
  • the first magnetic separation module 4 could also serve as a washing module of the substance-nanoparticle complexes. Specifically, once the liquid containing the aforesaid complexes has flowed out, and with the magnetic element still magnetised, a magnetically inert washing liquid could be introduced into the separation chamber 6 in order to remove any unwanted substances from, the complexes.
  • a separate washing module for example having a magnetic functioning according to a mode constructively similar to the above-mentioned first module, or suitable to cause the separation of the complexes according to a different principle, for example, physical, chemical or chemical-physical.
  • the washing in the first magnetic separation module 4, or in the separate washing module could be pressurised, for example by spraying.
  • the separation chamber 6 and the mixing module 2 communicate via a first conduit 36, along which at least a first pump 38 is optionally arranged.
  • the apparatus 1 could comprise a plurality of first magnetic separation modules 4, for example arranged mutually in parallel, suppliable through the mixing module 2.
  • the apparatus 1 could comprise (not illustrated) deviating means in order to direct the liquid phase containing the substance-nanoparticle complexes - also defined stable colloidal suspension of the substance-nanoparticle complexes - towards the different modules, in a selective manner.
  • the deviating means could comprise one or more solenoid valves.
  • the separation chamber 6 is supported in an oscillating way with respect to the first magnetic separation module 4, in order to distribute the contents of this chamber (preferably uniformly) .
  • the separation chamber 6 could be reciprocating in a plane, or it could be rotatable about at least a rotation axis.
  • the apparatus 1 comprises at least a splitting module 10 of the substance-nanoparticle complexes which defines a mixing compartment 12 communicating with the separation chamber 6 and which contains one or more release reactants of the target substance from said complexes.
  • the aforesaid complexes in the stable colloidal suspension are again separated into their constituents (which is to say, target substance, magnetic nanopart icles and liquid) , in particular through the action of at least one release reactant.
  • the nanoparticles disconnected from the target substance remain in a colloidal solution of magnetic nanoparticles .
  • the transport of the substance-nanoparticle complexes from the separation chamber 6 to the splitting module 10 could occur by means of a further liquid (for example a washing liquid) supplied to the aforesaid chamber 6, in particular, after the first magnetic element 8 has been deactivated or moved sufficiently away from the chamber (in this regard, see below) .
  • a further liquid for example a washing liquid
  • a mechanical mixing member could be inserted.
  • the apparatus 1 could comprise a splitting module 10 alongside the mixing module 2.
  • each module 2, 10 could include its own mechanical mixing member 28, 28' wherein, preferably, these members may be moved by motor means 70 common to both.
  • the washing liquid tank is indicated by the number 40.
  • the second conduit that leads from this tank 40 to the separation chamber 6 is instead indicated by the number 42.
  • From the separation chamber 6 to the splitting module 10, the connection is instead made through a third conduit 46.
  • at least one pump 44,48 could be arranged.
  • the release reactant could be a saline solution at suitable pH, composition and concentration, or one or more organic solvents.
  • the release reactant is fed via a reactant tank 50 and a respective fourth conduit 52 terminating in the splitting module 10.
  • the release reactant could comprise a buffer solution of suitable pH, composition and concentration, or one or more organic solvents.
  • the release reactant could comprise a concentration between 0.5 and 2.5 M of NaOH, NaCl, KC1, KOH, NH40H, NH4C1 or mixtures thereof, and ethanol between 10 and 100%wt.
  • the apparatus 1 also comprises at least a second magnetic separation module 14, placed fluidically downstream of the splitting module 10, to separate the. target substance from a stable colloidal liquid suspension of magnetic nanoparticles.
  • the target substance is by now disconnected from the magnetic nanoparticles and the latter are returned in stable colloidal suspension, in the second magnetic separation module 14 the mixture is again separated to isolate the target substance.
  • the magnetic nanoparticles will be magnetically anchored in the module analogously to what was discussed with regard to the first magnetic separation module, while the target substance and the liquid of the said suspension will not undergo any magnetic influence and can be separated from the magnetic nanoparticles, for example by flowing out from the module, in particular for a possible subsequent purification .
  • splitting module 10 and the second separation module 14 could be connected by means of a sixth conduit 54, along which is optionally arranged a pump 56.
  • the second magnetic separation module 14 comprises a recovery chamber 16, located downstream of the splitting module 10 to receive from the latter the target substance divided from the magnetic nanoparticles, and at least a second magnetic element 18 to attract the magnetic nanoparticles.
  • the second separation module 14 is . fluidically connected to the mixing module 2 so as to create a recirculation of at least part of the stable colloidal liquid suspension of magnetic nanoparticles .
  • the transport of the magnetic nanopartxcles in stable liquid suspension from the second separation module 14 to the mixing module 2 could be performed through a seventh conduit 62, effecting the related transport by means of a further liquid.
  • the further liquid could comprise the washing liquid coming from the tank 40, through an eighth conduit 64 terminating in the second separation module 14.
  • a collection tank 72 inside which can be made to flow the magnetic nanoparticles in stable liquid suspension, for example through the seventh conduit 62, optionally effecting the transport by means of. a further liquid (for example the washing liquid) .
  • the target substance it may finally be conveyed to a product tank 64 through a ninth conduit 66 exiting from the recovery chamber 16.
  • a pump 68 could be arranged along this conduit 66 .
  • the first magnetic element 8 (and optionally the second magnetic element 18) is functionally connected to a bottom wall 20 of the separation chamber 6 (and optionally to a bottom wall 22 of the recovery chamber 16) , where the first magnetic element (and optionally the second magnetic element) and the aforesaid wall could be moved together/apart to modulate the intensity of the magnetic field acting on the substance-nanoparticle complexes (and optionally on the magnetic nanoparticles ) .
  • the first magnetic element 8 (and optionally the second magnetic element 18) comprises the magnetised plate 74 placed below the separation chamber 6 (and optionally below the recovery chamber 16) , and where the bottom wall 20 of the separation chamber 6 (and optionally the bottom wall 22 of the recovery chamber 16) is movable in relation to the aforesaid plate 74 to modulate the magnetic field.
  • apparatus 1 could comprise, as first magnetic element and/or as second magnetic element, one or ' more electromagnets operatively connected to a bottom wall of the separation chamber and/or to a bottom wall of the recovery chamber, wherein the intensity of the magnetic field acting on the substance-nanoparticle complexes and/or on the magnetic nanoparticles is adjustable by modulating the current intensity fed to the electromagnet or to the plurality thereof .
  • the magnetic field in correspondence, of the bottom wall 20, 22 of at least one of the modules 4, 14 described above is preferably adjusted in intensity to a value equal to, or smaller than, about 150 millitesla (for example in a range between 20-80 millitesla), in order to promote a re-suspension of the substance- nanoparticle complexes and/or of magnetic nanoparticles in a stable colloidal suspension.
  • At least one of said walls could have a surface roughness equal to or less than 0.01 Ra mm (for example of approximately 0.001 to 0.005 Ra mm), in order to limit the surface adhesion of the substance- nanoparticle complexes and/or of the magnetic nanoparticles .
  • At least the bottom wall 20 of the separation chamber 6 and/or at least the bottom wall 22 of the recovery chamber 16 could comprise a glass surface, which interiorly delimits the separation chamber 6 and/or the recovery chamber 16.
  • At least the bottom wall 20 of the separation chamber 6 and/or at least the bottom wall 22 of the recovery chamber 16 could comprise a non-stick surface, in .order to reduce the possible surface adhesion phenomena of the magnetic nanoparticles and/or of the substance-nanoparticle complexes.
  • the liquid head is adjusted so as to reach a maximum level equal to, or smaller than, about 7 mm, for example 0.1 to 5 mm.
  • such level adjustment could be. effected through the first pump 38, optionally connected from the operational point of view to (not illustrated) management and control means of the apparatus 1.
  • the separation chamber 6 and/or the recovery chamber 16 could be inclinable (for example laterally), in order to make flow out, naturally or forcibly, a washing liquid, only the liquid of the aforesaid suspension, and/or the stable colloidal suspension containing the magnetic nanoparticles and/or the substance-nanoparticle complexes .
  • such outflow of the washing liquid and/or of the liquid of the suspension could be effected when the first 8 and/or the second 18 magnetic element is activated and/or positioned to magnetically retain the substance-nanoparticle complexes and/or the magnetic nanoparticles .
  • the apparatus 1 comprises oscillation motor means 24, 76 of the separation chamber 6, and optionally oscillation motor means 24', 76' of the recovery chamber 16.
  • the apparatus 1 comprises first oscillation motor means 24, 24' for moving the separation chamber 6 and/or the recovery chamber 16 in a plane substantially parallel to the bottom wall 20, 22 of this chamber.
  • the apparatus 1 comprises second oscillation motor means 76, 76' for moving the separation chamber 6 and/or the recovery chamber 16 in a plane substantially orthogonal to the bottom wall 20, 22 of this chamber.
  • Figure 2a shows the Cartesian system with respect to which the first oscillation motor means 24, 24' are configured to move the chamber 6, 16 in the plane Y, Z, while the second oscillation motor means 76, 76' are delegated to perform movements of this chamber in the plane X, Y.
  • the apparatus could include approaching/distancing motor means 26 between the first magnetic element 8 and the bottom wall 20, and/or approaching/distancing motor means 26' between the second magnetic element 18 and the bottom wall 20.
  • the apparatus 1 could comprise management and control means operatively connected to the oscillation motor means 24, 24', 76, 76' and/or the approaching/distancing motor means 26, 26' for controlling these means 24, 24', 76, 76', 26, 26' in a mutually independent way.
  • the management and control means could be functionally connected to one or more pumps among those described above and/or the deviating means, when provided.
  • the oscillation motor means and/or the approaching/distancing motor means could comprise one or more linear actuators for moving the respective chamber 6, 16, the respective magnetic element 8, 18 and/or the respective bottom wall 20, 22.
  • one or more linear actuators may be of the pneumatic, hydraulic, mechanical and/or electromechanical type.
  • This invention also relates to a method for separating at least one target substance from a liquid phase.
  • the method covered by this invention comprises ' steps of :
  • the method comprises a step of re-introducing in step i) at least part of the magnetic nanoparticles of step iv) in a stable colloidal suspension in order to perform a recirculation thereof.
  • steps ii) and iv) comprise a step of modulating the intensity of the magnetic field acting on the substance- nanoparticle complexes and/or on the magnetic nanoparticles , by moving a magnetic element (for example permanent) towards/away from said complexes and/or said nanoparticles , or by adjusting the current intensity fed to one or more electromagnets.
  • step ii) and/or step iv) comprise a step of magnetically retaining the substance-nanoparticle complexes and/or magnetic nanoparticles, and a step of making flow out, naturally or forcibly, the liquid only of the aforesaid suspension.
  • step i) comprises a sub-step of functionalising the magnetic nanoparticles, directly or indirectly.
  • the magnetic nanoparticles comprise or consist of superparamagnetic nanoparticles of stoichiometrically pure maghemite.
  • such nanoparticles are capable of creating stable colloidal suspensions without any coating in the aqueous phase.
  • said nanoparticles are configured to give rise to stable colloidal suspensions in aqueous phase by treatment with ultrasonic bath.
  • the apparatus and the above method allow separating a potentially infinite variety of target substances, in a simple and repeatable way and at an extremely low cost.
  • the stable colloidal suspensions of the nanoparticles of this invention allow their easy production on an industrial scale.
  • the nanoparticles used in this invention are suitable for forming stable colloidal suspensions in water, and have a surface reactivity suitable to reversibly bind organic or biological molecules of any kind.
  • the apparatus and method of this invention method could be used, without affecting the colloidal stability of the colloidal suspensions of nanoparticles in the process of separation of. the target substance, 'for the reclamation of civil or industrial water on a large scale.
  • the apparatus of this invention is designed to minimise the amount of residual substances in the treatment chambers.
  • the apparatus and the method of this invention allow attracting the nanoparticles and the complexes formed by them in an intelligent way, without damaging the stability of the re-suspensions of such particles.
  • the apparatus of this invention is designed to deploy the components in a homogeneous way inside the treatment tanks, to improve the yield and separation times of the various substances.
  • the apparatus and method of this invention method could be automated, so as to speed up and optimise the separation . times of the target substances.

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  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

L'invention concerne un appareil (1) pour séparer des substances cibles d'une phase liquide comprenant un module de mélange (2) pour mélanger une substance cible et une suspension liquide colloïdale stable de nanoparticules magnétiques de façon à obtenir des complexes substance-nanoparticule, un premier module de séparation magnétique (4) comprenant une chambre de séparation (6) et au moins un premier élément magnétique (8) fonctionnellement relié à ladite chambre (6) pour attirer magnétiquement les complexes, un module de fractionnement (10) pour fractionner les complexes substance-nanoparticule contenant un ou plusieurs réactifs de libération de la substance cible des complexes, et un second module de séparation magnétique (14), placé fluidiquement en aval du module de fractionnement (10), pour séparer la substance cible d'une suspension liquide colloïdale stable de nanoparticules magnétiques.
PCT/IB2016/051605 2015-04-03 2016-03-22 Appareil et procédé pour une séparation par le biais de nanoparticules magnétiques WO2016157027A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201680020361.XA CN107635666B (zh) 2015-04-03 2016-03-22 用于通过磁性纳米颗粒分离的装置和方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITBS20150056 2015-04-03
ITBS2015A000056 2015-04-03

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WO2016157027A1 true WO2016157027A1 (fr) 2016-10-06

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JP7139931B2 (ja) * 2018-12-14 2022-09-21 株式会社島津製作所 磁性体粒子操作用容器及び磁性体粒子操作用装置

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