WO2016118023A1

WO2016118023A1 - The method of functional biomimetic activation of an organism's vital functions

Info

Publication number: WO2016118023A1
Application number: PCT/PL2015/000005
Authority: WO
Inventors: Serhii MASIUK
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-01-19
Filing date: 2015-01-19
Publication date: 2016-07-28
Anticipated expiration: 2017-07-19
Also published as: WO2016118023A8

Abstract

The object of the invention is a method of functional biomimetic activation of an organism's vital functions. The method involves supplying a biomimetic electret obtained from substances with dielectric properties of inorganic or/and organic origin exposed for a period of 0.1 to 3600 minutes to pulse electromagnetic field with pulse period lasting 0.005 to 200 ms and pulse repetition frequency of 0.01 Hz to 300 GHz, showing neuro-like biotropic pulses - positive, negative or bipolar, and magnetic induction value ranging from 0.1 pT to 200 μΤ.

Description

The method of functional biomimetic activation of an organism's vital functions

The object of the invention is a method of functional biomimetic activation of an organism's vital functions. The invention is a solution in the field of biophysics and biomimetics.

According to general knowledge, weak electromagnetic fields of extremely low frequency or quasi-constant electromagnetic fields which are or may be generated by all living organisms, are involved in the mechanisms of regulating and controlling growth, reproduction, behavior, ageing and other life processes.

Patent description U 2136318 discloses a method to be followed in wound treatment which involves the use of a three-layer film coating which consists of outside vegetable polysaccharide layer, middle collagen layer, and a layer of fine demineralized bone matrix chips which touches the wound. The method improves the wound healing process which is related to increased sorption capacity of film coating and in consequence activates and accelerates the recovery process.

The volume of cells and tissues is penetrated by local fields and to the same extent by electromagnetic field which depends on a bioelectric phenomenon known under various names such as direct current, constant potential, metabolic potential, potential of a basic exchange gradient hereinafter referred to as PS. In human, animal and plant tissues PS voltage ranges from 10^"2— 10 ¹ V, and density from 10^~6— 10 ^s A/mm². To generate PS in human skin epidermal cells lose 5-10% of the energy from oxidation processes. PS parameters correlate with growth, regeneration and functional activity of organs and tissues but are not sufficient to directly impact the course of biological processes.

Electric charges are characteristic for interfacial potential difference where the biological tissue comes in contact with the external environment, as well as for natural electric polarization of its structures. What is necessary to generate external long-term electromagnetic field in the latter case is the state of a non-uniform polarization corresponding to non-uniform polarization of electrets. Electrets are dielectrics and certain semi-conductors in which electric polarization was generated through exposure to electrical or magnetic field, nuclear radiation, light and other factors, and in which charges were captured in traps - structure defects. A characteristic property of an electret is an external long-term electric field. What is characteristic for the matter of biostructures is natural electric polarization. It is formed by an organized distribution of polar molecules of proteins, nucleic acids, phospholipids, polysaccharides, water and other substances showing dipole moments with values ranging from few debye units (D) in water molecules to tens of thousands debye (D) in nucleic acids molecules. During the formation of supramolecular, subcellular and cellular structures the total dipole moment of a higher-level structure increases. And thus in the supramolecular structure of a bacteriophage it reaches the level of 10⁵D, and 10⁶D in a bacterial cell. Electric polarization vectors in cells form a complicated architecture of vectors of macroscopic electric polarization of elements of animal and plant tissues. The similarity of non-equivalent electric polarization of biostructures and electrets was explained as a result of research conducted and presented in a series of papers [Kulin E.T.: Electromagnetic fields of biosystems and life processes, Minsk Medical Innovations, 1998, No. 10. pages 24-29. ; Nature 442, 457-460 (27 July 2006); ELECTRETS Edited by G.M.Sessler/Topics in Applied Physics. Springer-Verlag- Heidelberg-New York 1980], which proved that all living organisms, from the most complex to the least complex without exception, behave and may be considered to be electrets - bioelectrets.

The generation of an electromagnetic field of bioelectret origin in humans hereinafter referred to as the bioelectret field, depends directly on molecular regeneration of biostructures. In case of hypoxia in human tissues involving insufficient supply of oxygen to tissues which is a result of reduced oxygen diffusion in the lungs or abnormal transport of oxygen in blood to tissues, the intensity of molecular regeneration of their biostructures and the intensity of this field decrease, after a few hours reaching values lower by a range from the initial value.

The same relationship was discovered during animal experiments. In case of hypoxia and during a drug-induced sleep the intensity of molecular regeneration of biostructure in the tissue of rats and the intensity of the bioelectret field decrease. Electrical irritation of the skin in rats increases the intensity of molecular regeneration by 1.5 times, and the intensity of the field twice. Ultraviolet treatment of human skin changes the course of its metabolic processes, particularly in basal cells and spinous cells of the epidermis, and the intensity of the bioelectret field is also changed significantly. It shows that processes occurring in the epidermis have the primary influence on bioelectret field recorded close to a human body. Electromagnetic fields weaken considerably when they penetrate tissues located deeper first going through tissues located above. For instance, reasearch showed that electromagnetic field of frequency of 5 Hz decreases thousandfold when penetrating brain tissue of the thickness of 0.1mm.

A characteristic feature of the electret state of the substance - thermally stimulated currents (TSC) was discovered in porcupine quills and in bull horns by E. Menefee (Kulin E.T.: Electromagnetic fields of biosystems and life processes, Minsk, Medical Innovations, 1998, No.10.), and identified it as a feature of natural electret state of biological tissues. Then two TSC peaks were observed in keratine structures which prove the existence of two electric polarization systems in their matter. Thus, the electrate state in biostructures exists also if there is no molecular regeneration which occurs in living organisms. And thus in leaves of Crassula Portulacea plant (Crassula), the intensity of the bioelectret field and the value of the TSC peak decrease when metabolism is disrupted as a result of their dehydration.

Thus the following two properties of the electret state are characteristic for natural electric polarization of biostructures: long-term external electromagnetic field and thermally stimulated currents TSC. In living biostructures this state depends on the molecular regeneration of their matter, whereas in dead biological structures it is analogous to the state of the matter in artificially produced electrets. The possibility of creating the electret state of the matter in biological objects (biological membranes, protein, polysaccharide preparations etc.) artificially was shown in a number of research studies.

One of the characteristic features of the bioelectret state - bioelectret field BE - in a human organism is the occurence of pulses in the frequency range of 10^"5— 10^"1 Hz. Pulses in periods lasting around one hour (30-70 minutes), characteristic for human and rat organism coincide with the above-mentioned approximately one- hour fluctuations of the intensity of the molecular regeneration of proteins. Changes in the amplitude value of field intensity fluctuations are synchronic and decrease significantly with the increase of frequency. For example, 5 centimeters from a human body the voltage value of a quasi-constant component of the field is at the level of thousands mill volts, and the amplitude value of field intensity fluctuations of the frequency of 10^"2 and 10^"1 Hz reach tens of millivolts and single millivolts respectively.

During calm wakefulness in practically healthy people the amplitude of bioelectret field intensity fluctuations of several-hour periodicity correlates with fluctuations of an organism's physiological activity. The fluctuations are synchronic over most parts of the body and take the form of slow pulsation of the entire organism's field. Generally, in case of each person a zone of maximum field intensity is recorded over one body area, and a zone of minimum field intensity is recorded over another area. Those zones migrate over body areas, remaining in one of them from 1 to 7 days. In most subjects the zone of maximum field intensity lasted longer in the area of the head.

The intensity of bioelectret field at the level of supramolecular structures reaches the value of 10⁸ V/m, and as the distance grows, the value decreases and at the distance of 10^"2 m it assumes values of 10⁵ V/m. Conduction currents flow in cells and tissues in line with the potential gradient of this field. These are long- known conduction currents CC in biological tissues. In such a case, what is characteristic for them and electret currents are changes of the type of thermally stimulated currents TSC which were proved experimentally. Thus, for example when heating 7-day winter wheat sprouts, the conduction current in their tissues increases and in a specific temperature range undergoes analogous changes as thermally stimulated currents TSC of carnauba wax magnetic electret. Bioelectret origin of the conduction current is proved by the correlation of the dynamics of bioelectret field intensity and the intensity of the conduction current in human forearm. What supports the fact that the physical basis for the generation of the conduction current is the electric polarization of biostructures, is the drop in conduction current intensity and pyroelectric voltage in the epidermis of a cockroach with hypoxia, as well as correlation of conduction current value and piezoelectric intensity in human upper limb bones. Research results and data available in reference literature confirm that conduction current in tissues, discovered in mid 20th century, is one of the manifestations of electret-similar state of living tissues. Consequently, bioelectret state is manifested by bioelectret field, conduction current, thermally stimulated current and pyro- and piezoelectric intensity.

The publication by SUN S. , KOU T., ZHU H. showed that collagen is also a bioelectret (SUN S. , KOU T., ZHU H. : "A study on bioelectret collagen", Research Center of Material Science, Beijing Institute of Technology, Beijing 100081, China, Journal of Applied Polymer Science, ISSN 0021-8995, 1997, vol. 64, n°2, pp. 267- 27.). The paper describes the use of collagen as bioelectret to accelerate wound healing. Bioelectret material based on collagen was prepared, then the processes of thermally stimulated discharge of collagen were measured under different conditions. Cell culture studies were used in the determination of the suitability of this kind of bioelectret as surfaces for the attachment and growth of cells. Results confirmed the potential use of bioelectret collagen in soft tissue wound healing.

Electret properties of human blood were also confirmed by research using thermally stimulated current TSC (L.S. Pinczuk, A.G. Krawcow, S.W. Zotow: "Thermally stimulated depolarization of human blood", Technical Physics, 2001, vol. 71, issue 5).

The above-mentioned values of the internal bioelectret field intensity are sufficient to influence the course of biochemical processes, movement of polar fluids, protein molecules, nucleic acids, moving supramolecular and intracell structures and cells. It was found that DNA synthesis in chondrocytes is stimulated by an electromagnetic field EM of the intensity of 10⁵ V/m. The intensity of the field, along the lines of which fibroblasts migrate, is 10² V/m. Field intensity of 10 V/m is sufficient to change the growth direction of nerve cells. Electromagnetic fields EM of bone tissue, dependent on its piezoelectric intensity activate osteoclasts and osteoblasts - cells, destroying and forming intercellular bone matrix. Furthermore, a field with a negative potential in relation to the earth's potential, activates osteoblasts and in consequence the process of bone tissue regeneration.

In a cell the energy of its internal field is obviously transformed into kinetic energy from the movement of ions, mobile supramolecular and intracell elements (electrophoresis), and the movement of the liquid part of cytoplasm (cytosol) in capillary subcellular spaces (electroosmosis). These phenomena are observed during the analysis of the phenomenology of intracellular dynamics which is regarded as one of the fundamental life processes. Hence in a typical hepatocyte of homeotherms thousands of mitochondria and transport vesicles, hundreds of lysosomes, paroxysms, endosomes and other micromolecules constantly move with different velocity in various directions, along different trajectories, however they may move along the same trajectory as opposed to one another. Furthermore, cytosol streams move constantly. If intracellular movement is stopped, life processes will cease. Hence, stopped flow of chloroplasts in a plant cell indicates that it is dead. Phenomenology of intracellular movement of micromolecules corresponds to electrophoresis, and cytosol to the phenomenology of electroosmosis.

Modulation of frequency and amplitude of bioelectret field and correlation of its parameters with the course of biological processes show that bioelectret field BE may be a carrier of biologically important information on life processes (bioinformation). The fact of using bioinformation included in electromagnetic fields of very low frequency of aquatic organisms is known and has been described in literature (Ivanov A.A: Fish physiology, ed. MIR, 2003, chapter 2). Some fish such as sharks have electroreceptors with sensitivity threshold of 10^"5— 10^"6 V/m and use them to detect the electromagnetic field of other fish while searching for a mate or prey. Sensitivity of electroreceptors in this fish makes it possible to identify the intensity of human bioelectret field in the air at the distance of 3 to 10 km.

The intensity of bioelectret field in cells and tissues is sufficient to influence the course of biochemical processes and displace ions, liquids and mobile elements. Furthermore, bioelectret field undoubtedly includes bioinformation which may be used for non-invasive diagnostics and therapy of processes in an organism (Nature 442, 457-460 (27 July 2006).

Processes occurring in human and animal epidermis, and in particular in basal and epithelial cells, are interrelated to processes in internal organs and in an organism as a whole. The state of processes in the mentioned cells, as shown above, reflects parameters of the bioelectret field, registered in the air in the vicinity of a human body. Parameters of this field include information on the state of processes in internal organs as is confirmed by the above-described correlations between the dynamics of bioelectret field intensity and processes occurring in organisms of practically healthy people with physical and psycho emotional tensions. The same phenomenon was observed during the analysis of processes in a sick organism.

Research on neurological symptoms of lumbar osteochondrosis showed that with effective treatment the intensity of the bioelectret field in a sick person has a growing tendency. Increase in the field intensity after an acupuncture session is correlated with a positive result of the treatment, and the reduction or lack of voltage increase with a lack of results. Based on this relation a method of predicting acupuncture efficiency was proposed.

It was assumed that changes in the intensity of bioelectret field resulting from acupuncture reflect the way an organism reacts to this kind of therapy. This assumption was confirmed by research on the dynamics of the intensity of bioelectret field in humans diagnosed by means of x-radiation. Research results showed that the dynamics of field intensity correlated with individual radio reactivity of a human organism.

As a result of molecular regeneration of a living substance, protein components of supramolecular and subcellular structures disintegrate constantly and are removed from the structures, and are then replaced by new synthesized protein molecules. Thanks to that the total dipole moments of biostructures change. For instance, the disintegration of protein molecules, with dipole moments ranging from 10²— 10³D, into peptide and amino acid molecules with dipole moments in the range of tens of debyes (D), or the leaving of the structure by a macromolecule results in the reduction of its total dipole moment provided that the macromolecules it is formed by are the same direction polarization vectors.

Velocities of decay (leaving) and incorporation of protein molecules into supramolecular structures are different which conditions quasi-periodic fluctuations of the process of their molecular regeneration. In particular, in liver and spleen cells of a rat and in the culture of liver cells the synthesis of proteins, incorporation and their leaving from supramolecular structures takes place regularly with a changing period ranging from 23-60 minutes ( approx. one-hour fluctuations) and with a high amplitude.

The dependence of the electric polarization of structures on the processes of molecular regeneration is reflected in its characteristics - the value of pyro- and piezoelectric intensity. Hence, in hypoxia the intensity of the molecular regeneration of biostructures decreases and the value of piezoelectric intensity in cockroach epidermis decreases accordingly. In hypoxia piezoelectric intensity in cochlea of the inner ear cells of a cat falls almost to zero which is known as Wever-, Bray effect.

In this way in living biostructures the natural electrical polarization constantly changes which means that it is not balanced. Non-balanced electrical polarization is the physical basis of electrets. As a result of energy fluctuations the electrical polarization of an eiectret changes and charges are constantly released from traps in form of structure defects. The so-called quasi-constant electrical charges which condition the characteristic property of the eiectret state of substance - external long-term electrical field are formed then. Conduction current flows in the internal electromagnetic field EM of an eiectret which increases when heated and changes significantly in a specific temperature range. This phenomenon has been called thermally stimulated current TSC. TSC together with the external electromagnetic field EM are characteristic properties of eiectret state of substance (E. T. Kulin Electromagnetic fields of biosystems and life processes, Minsk, Medical Innovations. - 1998. - no. 10. - pp. 24-29.; G.M. Sessler: ELECTRETS, Topics in Applied Physics, Springer-Verlag-Heidelberg-New York 1980).

Eiectret - dielectric on the surface or in the volume of which uncompensated electrical charges remain for a long time and create a quasi-static (slow-changing) electric field in the space surrounding the eiectret (G.M. Sessler: ELECTRETS, Topics in Applied Physics, Springer-Verlasg-Heidelberg-New York 1980).

The term eiectret was put forward by an English physicist Oliver Heaviside in 1896, and sample electrets were first obtained by a Japanese physicist M. Eguchi in 1919 from carnauba wax and rosin - polarized dielectrics cooled within a strong electric field, after heating them above their melting temperature . Electrets prepared based on this method are called thermal electrets. Thermal electrets can generate electrical field in the surrounding space over several months or even years. Thermal electrets manufactured from materials which show photoconductive properties when exposed to light and electrical field at the same time such as for example sulfur and cadmium sulfide.

The electret state is formed in a dielectric when electric charges are created, both positive and negative, on each surface of an electret placed between polarizing electrodes. When the polarization ends, the heterocharge is dominant and after some time when the thermal motion changes the orientation of dipoles, the homocharge becomes dominant.

The lifetime of electrets in normal conditions may last decades. It decreases quickly with the increase of temperature and ambient humidity. Heating releases charge carriers captured by traps - structure defects, neutralizes voluminal loads at the cost of electrical conductivity of a dielectric. In materials with very high specific resistance, relaxation of charge in deep traps being structure defects, is very slow.

At present electrets based on polymer membranes such as polytetrafluoroethylene, polyethylene terephthalate, polycarbonate and poly(methyl methacrylate) have the most practical applications in higher humidity conditions, the most stable are electrets from polytetrafluoroethylene (Yu.A. Kholodov, A.N. Kozlov, A.M. Gorbach, Magnetic fields of biological objects, Science, 1987).

Physiological effects, which occur when pulse currents are used, are a complex phenomenon, the character of which depends in the first place on their amplitude and frequency.

Current intensity amplitude in the range of 1 to 4 mA is thought to be the most effective. The lower limit of this range, which equals 1mA, is treated as the sensory threshold, while the upper limit of 4 mA is considered to be the pain threshold. Furthermore, it is assumed that pulse current below the sensory threshold (1mA) is useless as therapeutic means since it does not trigger any sensations which is incorrect from the point of view of bionics and biomimetics.

Biomimetics also referred to as bionics (biomimetics - from Latin bios - life and mimesis - imitation), a field of science which deals with the creation of devices, instruments, mechanisms or technologies, whose idea and basic elements are borrowed from nature. The following areas of biomimetics can be distinguished:

1) biological biomimetics, studying processes occurring in biological systems;

2) theoretical biomimetics, which builds mathematical models of these processes;

3) technical biomimetics, which applies models of theoretical biomimetics to solving engineering problems.

What is equally important to pulse current amplitude is its shape (steep front) and frequency. Electrophysical research shows that triangular pulses (Faradic current), with high front steepness (almost vertical), can only be used for electrical stimulation of healthy tissues, and have no effect on tissues with disturbed nourishment. It is related to the fact that tissues with neurotrophic damage do not keep up with responding to pulses of a given shape.

Neuro-like pulses of a special shape lasting from 0.005 to 200 ms with graphically-displayed wave form in the shape of a saw, plough or a sinusoid, with a steep rise or slope of the front steepness can work on both healthy tissues and tissues with neurotrophic damage.

Apart from the amplitude, shape and duration of an electrical pulse which should not be shorter than 0.005 ms if the intended positive result is to be obtained, an equal importance must be attached to the pulse train frequency measured in Hertz (Hz).

Thus, for example, electrical current with pulse train frequency in the range of 1-lOHz stimulates the sympathetic nervous system, sensory and motor receptors, whereas at the frequency of 100 Hz it blocks the activity of the sympathetic nervous system. With frequency ranging from 25-100 Hz stimulation of the parasympathetic nervous system and expansion of vessels is observed. The frequency of 20-30 Hz improves the process of neuromuscular transmission and is used in the treatment of paralytic muscular atrophy. Best anti-inflammatory and analgesic results are obtained at frequencies ranging from 80-250 Hz. The effect of electrical or electromagnetic pulses of various frequency is now the in the center of attention in connection with the expected non-amplitude character of the transmission of information in biological systems (frequency coding of neural information was discovered by I. P. Pavlov).

In particular, it turned out that human organism is able not only to respond in a wide range of frequencies (from 101 to 10 9 Hz and higher), but also to respond to specific frequencies in a specific way; still the amplitude of the signal may be small i.e. its values may be below the sensory threshold. Therefore the known and applied exposure of organisms to pulses with amplitude of tens and hundreds of volts which are generated by a number of devices in use, is not of physiological nature and may be harmful to an organism.

Below are presented primary human electrical and magnetic fields of low frequency (Gotovskiy M. Yu., Perov Yu. F., Chernetsova L V. "Bioresonance Therapy". Moscow ."lmedis"2008, p.176 )

Voltage Value of Frequency

[μν] magnetic range,

Electrical fields Magnetic fields

induction [Hz]

[pT]

Myocardium

Electrocardiogram 0,3-3000 Magnetocardiogram 50 0,5-100

Fetal electrocardiogram 5-50 Fetal magnetocardiogram 1-10 0,5-100

Skeletal musculature

Electromyograph 1-500 Magnetomyograph 10 0-2000

Brain

Electroencephalogram 1-300 Magnetoencephalogram 1 0,5-30

Potentials induced 1-100 Magnetic fields induced 0,1 0-60

Such a selective effect on the biological system which does not require a big energy input and assumes the activation of an organism's internal energy resources under specific frequency is called triggering (resonance) activity and is one of the types of neuromodulation therapy.

In order to initiate a given effect, not only the effect of electrical pulses based on electrodes placed on the skin or acupuncture needles but also induction coils (Helmholtz coils), which create pulsed electromagnetic field of specific intensity and frequency can be used, as well as waveguides of special construction.

Prior art discloses methods of recording electromagnetic fields and radiation on special media which are in different phase states during the recording, in order to evaluate the state of biological objects which are, for example" performed in VIS (GB 515866), IR or RF (DE 3605104) range on photo films and other field-sensitive media.

Publications US 5095901, US 5617856, US 6032063, RU 2154508 disclose methods of using electromagnetic fields and radiations in the wide range of frequencies for improvement of biological objects' state, for example to stimulate some human functions,

Known are methods of using electromagnetic fields and radiation in the wide range of frequencies for improvement and activation of plants (SU 1665952) or yeast cells (SU 1592328) productivity.

Known are also methods of using fields to influence different biological objects, both for vertebrate organisms RU 2105575, RU 2121334), and for invertebrate organisms, for example, microorganisms (RU 2146540, 2000). However, majority of known effects on biological objects is obtained by way of large and small structures whose impact constantly changes and is often accompanied by hidden or not sufficiently studied by-effects.

Patent description RU2066890 discloses a method of electret production wherein polymer foil such as polyethyleneterephthalate foil coated with copolymer or vinyl chloride and vinyl acetate is subjected to heat treatment at 90-120°C for 40- 120 min followed by exposure to corona discharge field at 20-45 kV and polarization time of 60-120 s during cooling process. Coating applied to polyethyeleneterephthalate film is formed from 5-7% solution of copolymer in acetone to obtain electrets with initial electret potential difference 15 times higher than that of known electrets and higher time stability of charge.

Patent description U 2065295 discloses a method of diagnostics and therapy of living organisms which involves the use of a preparation for activating vital functions of a biological object, activated by electromagnetic field processing based on a circuit able to generate an electromagnetic field, the medium being a substance selected from a group including a pharmaceutically acceptable powder or pill substrate having crystalline substance in the amount of at least 23% by mass, physiologic fluid or liquid crystals. The medium is treated with electromagnetic field of the frequency of 0.01 Hz - 9,0 Hz, 10,0 Hz - 100,0 Hz or 0.5 Hz - 10 MHz during 0.5-60 min. Powder or pill substrate contains the mixture of at least two substances selected from a group including: talc, starch, dextrin, polyethylene glycol starch syrup, magnesium oxide, colloidal silicon dioxide, cellulose or its derivatives, and lactose or its derivatives and auxiliary substances for tableting and pharmaceutically acceptable colorants for labeling degree of activation of the electromagnetic field carrier. Crystalline substances used include non-organic potassium, magnesium, calcium or sodium salts, saccharin, glucose, fructose, maltose, lactose, rhamnose, ribose, dextrose or their mixture. The preparation includes physiological liquid in form of the physiological solution of sodium chloride or substrate diluted with drinking water by a ratio of 1: (5-10) selected from a group including Eagle's medium, Ringer's solution, Bordet-Gengou agar, Levinthal's medium, Field's medium, Zeissler's medium, serum agar and beef-extract broth.

Patent description RU2283090 discloses a preparation for stimulating the vital activity of biological objects and the method of its production. The preparation comprises a carrier processed by an electromagnetic field. The carrier is a substance selected from a group consisting of pharmaceutically acceptable powder or a substance for tableting containing not less than 23% by weight of crystal substance, physiological liquid and liquid crystals. The carrier has been processed by electromagnetic field EMF with a frequency within one of the ranges 0.01 Hz-9.0 kHz, 10.0-100.0 kHz or 0.5-10.0 MHz for a duration from 0.5 to 60 minutes. The preparation keeps stable properties over a period of one year, has an increased effectiveness and may be useful for future medical applications.

Known methods of producing electrets make it possible to obtain an electret which does not correspond to the physiological state of an organism and in no way imitates electret properties of healthy biomaterials as regards quality and quantity.

Russian patent description RU 2065297 discloses bioresonance therapy, whereas international patent application PCT/SU89/000113 describes a method of generating electrical pulses for stimulating biological objects, and the structure of a device to accomplish it, based on the effect of biological field of electromagnetic and electric origin on an organism, including also neuro-like and biotropic signals. The method consists in generating pulses of a predetermined frequency, modulating each pulse duration, producing a series of stimulating pulses of a predetermined duration of the series and of the length of the pauses between the pulses and amplifying the stimulating pulses of the series. The duration of the series of pulses applied to the object is varied and depends on the evaluation of the electrophysiological parameters of conditions in the interelectrode zone of the biological object which are determined simultaneously with the stimulating process. The device comprises a pulse generator, a pulse duration modulator, a pulse power amplifier, a first generator producing a signal determining the reaction of the object to the stimulating pulse, a generator for producing the pulse series of varying duration, a second generator for producing a signal whose duration corresponds to that of the series, and a limiter of the maximum level of the signal used for the modulation control.

None of the known inventions has solved the problem of stable effect on a biological object by means of a universal, portable, biologically similar biomimetic means for activation of biological object's vital functions, which would provide relatively comparable influence of electromagnetic field on various living organisms.

The objective of this invention is to create a universal method of stimulating vital functions of a biological object by means of a physiological, biochemically active portable carrier which may become a carrier of a charge with density and quality acceptable to an organism and quality to operate in a wide range of electromagnetic field and maintain stable original properties over a long period of time.

According to the invention, the method of functional biomimetic activation of an organism's vital functions involves supplying a biomimetic electret obtained from substances with dielectric properties of inorganic or/and organic origin exposed for a period of 0.1 to 3600 minutes to pulse electromagnetic field with pulse period lasting from 0.005 to 200 ms and pulse repetition frequency of 0.01 Hz to 300 GHz showing neuro-like biotropic pulses - positive, negative or bipolar, and magnetic induction value ranging from 0.1 pT to 200 μΤ.

Preferably the dielectric substance is a compound selected from a group including water, inorganic and organic acids and bases, salts of inorganic and organic acids, esters, aluminosillicates, sugars, amino acids, alcohols, metal and non-metal oxides, either pure or in form of a solution.

In order to obtain the functional biomimetic activation of an organism's vital functions, the biomimetic electret is supplied intracorporeally and/or extracorporeal^ to organisms in pharmaceutically effective and acceptable doses.

Biomimetic electret is used externally and/or internally in organisms of mammals in a pharmaceutically acceptable form.

Particularly good effect is achieved when the biomimetic electret is used on a carrier in form of a plaster.

To achieve functional biomimetic activation of vital functions of plants, biomimetic electret is mixed with substrate for plants or fungi.

The method of functional biomimetic activation of an organism's vital functions has been presented in the following examples of its embodiment.

Example 1.

L-Glycine amino acid in form of a powder was placed in an electromagnetic field of a device able to generate electromagnetic field EMF known for example from Russian patent description RU no. 2070406 It was exposed to electromagnetic field EMF of the frequency of 100 kHz for 50 minutes. The obtained electret biomimetic preparation is able to keep electromagnetic properties for 12 months at room conditions.

Example 2.

Milk sugar - lactose - in form of granules, was put inside a loop of an electromagnetic field like in example 1 and was exposed to the field with intensity of 100 Hz, bipolar signal, with magnetic induction of 50 μΤ, exposure time 180s, the granules were then placed on the adhesive side of a plaster and was placed for the period of 72 hours on the spot where the patient felt joint pain. After that period the patient experienced a significant relief of joint pain and improved joint mobility.

Example 3.

Powder sodium bicarbonate was put in the loop of an electromagnetic field of a device referred to in example 1 and was exposed to electromagnetic field with frequency of 230Hz, bipolar signal, magnetic induction of 100 μΤ, exposure time 240s. The powder obtained was administered to a patient suffering from gastric hyperacidity with heartburn symptoms in doses of 0.3 g taken by mouth before a meal, after drinking water. After seven days decreased hyperacidity was observed and less unpleasant sensations in the stomach were observed, and the patient's general condition improved.

Example 4.

The effect of the preparation described in example 1, was investigated in experimental conditions on commercially available microorganisms, used in agriculture as producers of protein, amino acids and other food substances, used in animal production, as well as in food production, connected with a fermentation process. The investigation was performed on the following strains-producers of glutamic acid: Mycrococcus glutamicus, Brevibacterium flavum, Br. aminogenes, Br. saccharalyticum. During their cultivation under standard conditions, 1 ml of preparation was added to 10 ml of culture medium, at the same time a comparative culture was assayed where the preparation described in example 1 was used without activating its electromagnetic field EMF, and a second comparative culture was assayed without introducing the preparation referred to in example 1. In the experimental group the final product yield was by 17-25% higher than in the comparative group. Example 5.

Potassium chloride in the amount of 1 kilo was exposed to electromagnetic field with frequency of 47 Hz, unipolar input, negative pulse, magnetic induction of 100 μΤ, exposure time 30 minutes, the substance obtained was then mixed with spinach seeds and were planted in the ground along with a blank test consisting of a mix of spinach seeds and potassium chloride not processed in an electromagnetic loop. Seeds mixed with potassium chloride processed by electromagnetic field produced spinach more germinable and by approx. 50% bigger than the plants without potassium chloride exposed to electromagnetic field.

Example 6.

Sodium chloride in the amount of 5 kilos, was put in the loop of an electromagnetic field and exposed to field with frequency of 3 Hz, bipolar signal, magnetic induction 150 μΤ for 50 minutes, the salt obtained was then added to feed for lactating cows. Following the addition of the abovementioned preparation, the average milk yield during the lactation period increased by 10-15%.

Example 7.

Potassium sodium tartrate (Seignette's salt) in the amount of 1 kilo was put in the loop of an electromagnetic field and was exposed to electromagnetic field with the frequency of 7Hz, bipolar signal, magnetic induction of 50 μΤ for 30 minutes, and then the salt obtained was used as a laxative. Relaxation and patient's regular defecation were achieved as a result.

Example 8.

Sterile talc powder [Mg₃Si₄Oio(OH)₂] in the amount of 200 g was put in a loop of an electromagnetic field and was exposed to electromagnetic field with frequency of 18 Hz, bipolar signal, magnetic induction of 55 μΤ, for 5 minutes, the product was then used as baby powder for a specific group of children. The comparative group used sterile talc which was not processed in an electromagnetic field. It turned out that talc subjected to electromagnetic processing used by children in the group studied reduced chafe, and the condition of skin in children was better after using the powder as compared to the skin condition of children in the comparative group where the talc used was not subjected to electromagnetic processing.

Example 9. lOOg of amino acid-L-tryptophan was put in the loop and was subject to processing in an electromagnetic field with the frequency of 7.8 Hz, bipolar signal, magnetic induction value of 40 μΤ for 5 minutes, it was then used as an addition to soil - substrate for growing fungi - mushrooms (Agaricus arvensis). The production yield increased as a result.

Claims

1. The method of functional biomimetic activation of an organism's vital functions based on supplying an electret substance to an organism characterized in that a biomimetic electret obtained from a substance with dielectric properties of inorganic or/and organic origin exposed for a period of 0.1 to 3600 minutes to pulse electromagnetic field with pulse period lasting 0.005 to 200 ms and pulse repetition frequency of 0.01 Hz to 300 GHz, showing neuro-like biotropic pulses - positive, negative or bipolar, and magnetic induction value ranging from 0.1 pT to 200 μΤ, is supplied.

2. The method as claimed in claim 1, characterized in that the dielectric substance is a compound selected from a group including water, inorganic and organic acids and bases, salts of inorganic and organic acids, esters, aluminosilicates, sugars, amino acids, alcohols, metal and non-metal oxides, either pure or in form of a solution.

3. The method as claimed in claim 1 or claim 2, characterized in that the biomimetic electret is administered intracorporeally and/or extracorporeal^ to organisms in pharmaceutically effective and acceptable doses.

4. The method as claimed in claim 1, claim 2 or claim 3, characterized in that biomimetic electret is used externally and/or internally in organisms of mammals in a pharmaceutically acceptable form.

5. The method as claimed in claim 1 or claim 2, characterized in that the biomimetic electret is used on a carrier in form of a plaster.

6. The method as claimed in claim 1, characterized in that the biomimetic electret is mixed with a substrate for plants or fungi.