WO2016095934A3 - A novel genetic device to engineer cell behavior - Google Patents

A novel genetic device to engineer cell behavior Download PDF

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Publication number
WO2016095934A3
WO2016095934A3 PCT/EG2014/000038 EG2014000038W WO2016095934A3 WO 2016095934 A3 WO2016095934 A3 WO 2016095934A3 EG 2014000038 W EG2014000038 W EG 2014000038W WO 2016095934 A3 WO2016095934 A3 WO 2016095934A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell behavior
biomedical
production
novel genetic
novel
Prior art date
Application number
PCT/EG2014/000038
Other languages
French (fr)
Other versions
WO2016095934A2 (en
Inventor
Hisham Mohamed Magdy EL ABD
Original Assignee
El Abd Hisham Mohamed Magdy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by El Abd Hisham Mohamed Magdy filed Critical El Abd Hisham Mohamed Magdy
Priority to PCT/EG2014/000038 priority Critical patent/WO2016095934A2/en
Publication of WO2016095934A2 publication Critical patent/WO2016095934A2/en
Publication of WO2016095934A3 publication Critical patent/WO2016095934A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

This invention is related to the design and production of novel genetic device to control cell behavior. This device can be used to engineer eukaryotic and prokaryotic cells, so it has a wide variety of application from biotechnological to biomedical science and biomedical engendering. Also this invention is related to the production of novel genetic circuit and recombined DNA to control cell behavior. These genetic circuits and recombined DNA can be used to engineer eukaryotic and prokaryotic cells, so it has a wide variety of application from biotechnological to biomedical science and biomedical engendering. Also this invention is contains novel methods to solve some pharmaceutical, biopharmaceutical, and medical obstacles as miRNA delivery system and develop cancer therapeutics. Also this invention contains novel methods related to the production of synthetic promoters.
PCT/EG2014/000038 2014-12-14 2014-12-14 A novel genetic device to engineer cell behavior WO2016095934A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/EG2014/000038 WO2016095934A2 (en) 2014-12-14 2014-12-14 A novel genetic device to engineer cell behavior

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EG2014/000038 WO2016095934A2 (en) 2014-12-14 2014-12-14 A novel genetic device to engineer cell behavior

Publications (2)

Publication Number Publication Date
WO2016095934A2 WO2016095934A2 (en) 2016-06-23
WO2016095934A3 true WO2016095934A3 (en) 2017-07-13

Family

ID=56127775

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EG2014/000038 WO2016095934A2 (en) 2014-12-14 2014-12-14 A novel genetic device to engineer cell behavior

Country Status (1)

Country Link
WO (1) WO2016095934A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016095934A2 (en) * 2014-12-14 2016-06-23 El Abd Hisham Mohamed Magdy A novel genetic device to engineer cell behavior
IL271883B1 (en) * 2017-07-12 2024-04-01 Refuge Biotechnologies Inc Methods and systems for conditionally regulating gene expression
WO2020023767A1 (en) * 2018-07-26 2020-01-30 Joslin Diabetes Center Targeting micro-rnas for exosomal delivery or cellular retention
CN113227384B (en) * 2018-12-26 2023-06-06 深圳市亦诺微医药科技有限公司 Pharmaceutical compositions, kits and methods for treating tumors
WO2022096750A1 (en) * 2020-11-09 2022-05-12 ETH Zürich Expression system and method for controlling a network in a cell and cell comprising the expression system
EP3995577A1 (en) * 2020-11-09 2022-05-11 ETH Zurich Expression system and method for controlling a network in a cell and cell comprising the expression system

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6172279B1 (en) * 1989-01-26 2001-01-09 Zeneca Limited Plant gene construct encoding a protein capable of disrupting the biogenesis of viable pollen
US20090226404A1 (en) * 2005-12-09 2009-09-10 Argos Therapeutics, Inc. Methods for Generating Antigen-Specific Effector T Cells
US20090257991A1 (en) * 2008-04-09 2009-10-15 Maxcyte, Inc. Engineering and Delivery of Therapeutic Compositions of Freshly Isolated Cells
US20100285073A1 (en) * 2007-07-31 2010-11-11 The Board Of Regents, The University Of Texas System a micro-rna family that modulates fibrosis and uses thereof
WO2011093647A2 (en) * 2010-01-26 2011-08-04 Industry-Academic Cooperation Foundation, Yonsei University Genes implicated in osteoarthritis and use thereof
US20130007913A1 (en) * 2006-04-26 2013-01-03 June Medford Biological systems input-output response system and plant sentinels
US20130171626A1 (en) * 2012-01-04 2013-07-04 Massachusetts Institute Of Technology Methods of evaluating gene expression levels
WO2014117945A2 (en) * 2013-02-04 2014-08-07 Eth Zurich Designer circuit controlling diet-induced obesity
US20140342427A1 (en) * 2011-11-29 2014-11-20 The Regents Of The University Of California Photonic nanoantenna mediated gene circuit reconfiguration
US20140357530A1 (en) * 2012-12-12 2014-12-04 The Broad Institute Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
WO2016095934A2 (en) * 2014-12-14 2016-06-23 El Abd Hisham Mohamed Magdy A novel genetic device to engineer cell behavior

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6172279B1 (en) * 1989-01-26 2001-01-09 Zeneca Limited Plant gene construct encoding a protein capable of disrupting the biogenesis of viable pollen
US20090226404A1 (en) * 2005-12-09 2009-09-10 Argos Therapeutics, Inc. Methods for Generating Antigen-Specific Effector T Cells
US20130007913A1 (en) * 2006-04-26 2013-01-03 June Medford Biological systems input-output response system and plant sentinels
US20100285073A1 (en) * 2007-07-31 2010-11-11 The Board Of Regents, The University Of Texas System a micro-rna family that modulates fibrosis and uses thereof
US20090257991A1 (en) * 2008-04-09 2009-10-15 Maxcyte, Inc. Engineering and Delivery of Therapeutic Compositions of Freshly Isolated Cells
WO2011093647A2 (en) * 2010-01-26 2011-08-04 Industry-Academic Cooperation Foundation, Yonsei University Genes implicated in osteoarthritis and use thereof
US20140342427A1 (en) * 2011-11-29 2014-11-20 The Regents Of The University Of California Photonic nanoantenna mediated gene circuit reconfiguration
US20130171626A1 (en) * 2012-01-04 2013-07-04 Massachusetts Institute Of Technology Methods of evaluating gene expression levels
US20140357530A1 (en) * 2012-12-12 2014-12-04 The Broad Institute Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
WO2014117945A2 (en) * 2013-02-04 2014-08-07 Eth Zurich Designer circuit controlling diet-induced obesity
WO2016095934A2 (en) * 2014-12-14 2016-06-23 El Abd Hisham Mohamed Magdy A novel genetic device to engineer cell behavior

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WEISS ET AL.: "Genetic Circuit Building Blocks For Cellular Computation, Communications And Signal Processing", NATURAL COMPUTING, vol. 2, no. 1, 2003, pages 47 - 84, XP002662632 *

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