WO2016091765A3 - A method of rational antibody design - Google Patents

A method of rational antibody design Download PDF

Info

Publication number
WO2016091765A3
WO2016091765A3 PCT/EP2015/078728 EP2015078728W WO2016091765A3 WO 2016091765 A3 WO2016091765 A3 WO 2016091765A3 EP 2015078728 W EP2015078728 W EP 2015078728W WO 2016091765 A3 WO2016091765 A3 WO 2016091765A3
Authority
WO
WIPO (PCT)
Prior art keywords
describe
protein
antibody design
rational
rational antibody
Prior art date
Application number
PCT/EP2015/078728
Other languages
French (fr)
Other versions
WO2016091765A2 (en
Inventor
Michele Vendruscolo
Pietro SORMANNI
Francesco APRILE
Original Assignee
Cambridge Enterprise Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Enterprise Limited filed Critical Cambridge Enterprise Limited
Publication of WO2016091765A2 publication Critical patent/WO2016091765A2/en
Publication of WO2016091765A3 publication Critical patent/WO2016091765A3/en

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search

Landscapes

  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Theoretical Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Evolutionary Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

We describe a method for the rational design of complementary peptides that can bind with high specificity and affinity to target regions of a protein molecule or sequence. The method uses a computational algorithm that exploits the availability of a large number of experimentally-derived protein structures to identify potential interaction partners. We also describe grafting the designed sequences into protein scaffolds, such as an antibody scaffold.
PCT/EP2015/078728 2014-12-09 2015-12-04 A method of rational antibody design WO2016091765A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1421901.8 2014-12-09
GBGB1421901.8A GB201421901D0 (en) 2014-12-09 2014-12-09 A method of rational antibody design

Publications (2)

Publication Number Publication Date
WO2016091765A2 WO2016091765A2 (en) 2016-06-16
WO2016091765A3 true WO2016091765A3 (en) 2016-07-28

Family

ID=52425707

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2015/078728 WO2016091765A2 (en) 2014-12-09 2015-12-04 A method of rational antibody design

Country Status (2)

Country Link
GB (1) GB201421901D0 (en)
WO (1) WO2016091765A2 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU668423B2 (en) 1992-08-17 1996-05-02 Genentech Inc. Bispecific immunoadhesins
WO1997034631A1 (en) 1996-03-18 1997-09-25 Board Of Regents, The University Of Texas System Immunoglobin-like domains with increased half lives

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KENNETH L. BOST ET AL: "Complementary Peptides as Interactive Sites for Protein Binding", VIRAL IMMUNOLOGY., vol. 2, no. 4, 1 January 1989 (1989-01-01), US, pages 229 - 238, XP055259999, ISSN: 0882-8245, DOI: 10.1089/vim.1989.2.229 *
PIETRO SORMANNI ET AL: "Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 112, no. 32, 27 July 2015 (2015-07-27), US, pages 9902 - 9907, XP055246269, ISSN: 0027-8424, DOI: 10.1073/pnas.1422401112 *
R. J. PANTAZES ET AL: "OptCDR: a general computational method for the design of antibody complementarity determining regions for targeted epitope binding", PROTEIN ENGINEERING, DESIGN AND SELECTION, vol. 23, no. 11, 16 September 2010 (2010-09-16), GB, pages 849 - 858, XP055260005, ISSN: 1741-0126, DOI: 10.1093/protein/gzq061 *
ROBERT J PANTAZES ET AL: "MAPs: a database of modular antibody parts for predicting tertiary structures and designing affinity matured antibodies", BMC BIOINFORMATICS, BIOMED CENTRAL, LONDON, GB, vol. 14, no. 1, 30 May 2013 (2013-05-30), pages 168, XP021152858, ISSN: 1471-2105, DOI: 10.1186/1471-2105-14-168 *
TONG LI ET AL: "OptMAVEn - A New Framework for the de novo Design of Antibody Variable Region Models Targeting Specific Antigen Epitopes", PLOS ONE, vol. 9, no. 8, 25 August 2014 (2014-08-25), pages e105954, XP055260009, DOI: 10.1371/journal.pone.0105954 *

Also Published As

Publication number Publication date
GB201421901D0 (en) 2015-01-21
WO2016091765A2 (en) 2016-06-16

Similar Documents

Publication Publication Date Title
WO2017172645A3 (en) Bacteriophage engineering methods
WO2017178653A3 (en) NEW ANTI-SIRPa ANTIBODIES AND THEIR THERAPEUTIC APPLICATIONS
EA201790465A1 (en) ANTIBODIES, COMPOSITIONS AND APPLICATIONS
WO2017060475A3 (en) Polypeptides
WO2016077381A8 (en) Anti-interleukin-33 antibodies and uses thereof
PH12020550243A1 (en) Anti-tau antibodies and uses thereof
WO2016046778A3 (en) Protease-activatable bispecific proteins
WO2016149710A8 (en) Hiv-1 neutralizing antibodies and uses thereof
WO2017091811A3 (en) Methods for variant detection
WO2014150877A3 (en) Anti-tau antibodies and methods of use
WO2016061389A3 (en) Anti-alpha-synuclein antibodies and methods of use
WO2015196128A3 (en) Alternative nucleic acid molecules and uses thereof
WO2015196130A3 (en) Alternative nucleic acid molecules and uses thereof
WO2016110453A8 (en) A crispr-cas system for a filamentous fungal host cell
SG10201909716RA (en) Modified j-chain
GB2550745A (en) Crispr hybrid DNA/RNA Polynucleotides and methods of use
EP3104870A4 (en) Tau peptides, anti-tau antibodies, and methods of use thereof
WO2016207717A8 (en) Anti-vista antibodies and fragments
WO2015197823A3 (en) Antibodies and antigen-binding fragments that specifically bind to microtubule-associated protein tau
PH12018550160A1 (en) NEW ANTI-SIRPa ANTIBODIES AND THEIR THERAPEUTIC APPLICATIONS
EA201790505A1 (en) CONNECTION, AIMED ON IL-23A AND TNF-ALPHA, AND ITS APPLICATION
EP3426297A4 (en) Gitr antibodies, methods, and uses
WO2014197885A3 (en) Inhibitors of complement factor h
HK1244018A1 (en) Rabies virus g protein epitope, and rabies virus neutralising binding molecule that binds specifically thereto
WO2015153501A3 (en) Modified santalene synthase polypeptides, encoding nucleic acid molecules and uses thereof

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15816381

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 15816381

Country of ref document: EP

Kind code of ref document: A2