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WO2016089336A1 - Method for rapid diagnosis of benign and malignant neoplasms and associated diseases - Google Patents

Method for rapid diagnosis of benign and malignant neoplasms and associated diseases Download PDF

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Publication number
WO2016089336A1
WO2016089336A1 PCT/UA2015/000039 UA2015000039W WO2016089336A1 WO 2016089336 A1 WO2016089336 A1 WO 2016089336A1 UA 2015000039 W UA2015000039 W UA 2015000039W WO 2016089336 A1 WO2016089336 A1 WO 2016089336A1
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Prior art keywords
malignancy
benign
stages
system
neoplasms
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PCT/UA2015/000039
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French (fr)
Russian (ru)
Inventor
Николай Александрович ЩУКИН
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ЧЕРНОГУБОВ, Евгений Владимирович
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Application filed by ЧЕРНОГУБОВ, Евгений Владимирович filed Critical ЧЕРНОГУБОВ, Евгений Владимирович
Publication of WO2016089336A1 publication Critical patent/WO2016089336A1/en

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers

Abstract

The invention relates to medicine, and specifically to a method for the high-quality, rapid diagnosis of benign and malignant neoplasms and associated diseases by the microelectrophoresis examination of buccal epithelial cells. With the aim of increasing the accuracy of identifying benign and malignant neoplasms and associated diseases by the microelectrophoresis examination of buccal epithelial cells, the proposed method takes into consideration not only the cytomorphological and cyto-biophysical parameters of buccal epithelial cells, but also such parameters as: specific clustering of cells and the state of a sample, thereby increasing the accuracy and sensitivity of diagnosing benign and malignant neoplasms.

Description

METHOD FOR RAPID DIAGNOSIS benign and malignant tumors and related diseases

The invention relates to medicine, namely to a method of qualitative rapid diagnosis of benign and malignant diseases and related novobrazovany mikroelektroforeznym study buccal epithelial cells.

Background of the Invention.

Known is a method of qualitative differential rapid diagnosis of neoplasm of parotid gland content patient biomarkers in oral liquid [1]. The method involves examining oral patient fluid, wherein the performance sampling oral fluid in the patient is made before, or not earlier than 30 minutes after administration to the patient food centrifugation oral patient fluid at 3000 rev / min for 15 minutes, dilution of the oral fluid with saline in the ratio 1: 100 and re-centrifugation at 3000 rev / min for 15 minutes, ready for occupancy studies parotid gland material into a cuvette and performing the standard method of enzyme immunoassay ana Lease on die based on monoclonal antibodies, followed by analysis of the results of studies with oral fluid taken from the patient for diagnosis count content biomarkers in saliva, and then results obtained by determination of biomarkers in a patient's oral fluid predict patient management. The method provides a significant increase in accuracy, sensitivity differentsiatnoy rapid diagnosis, achieving a high probability of detection processes of benign and malignant tumors in the body of the patient as early in the disease, and in the later, as well as a substantial simplification of preparation patient within a time for collection and storage diagnostic material.

However, this method has the following disadvantages:

- highly specialized, which in turn does not allow to identify benign and malignant tumors of different origin and localization;

- does not allow to identify related oncological diseases. Also known method for diagnosing benign and malignant tumors [2]. A method for diagnosing cancer, comprising the laboratory studies conducted studies following testing parameters: the shape of cells, the membrane, the shape of the cell nucleus, the membrane of the cell nucleus color of the cell nucleus, incorporation into the cell cytoplasm, characterized ger nuclear motion, the amplitude of motion of the cell nuclei buccal epithelium of the patient, and then on the basis of formulas discriminant analysis, which are as follows:

DF 1 = 0,413 * X1 0,03 * X2 + 1 070 * HZ + 1, 325 0.243 * X4 * X5 + 0, 156 1 * X6, X7 + 43 * 0.345 * 0.076 * X8 + X9 + X10 + 0.357 * X1 0.342 * 1 + 0.236 * 0.78 * X12 + X 13- 19.657;

DF2 = 0,3 1 * X1 + X2 * 0.303 0.890 * HZ - 0.941 * 0.019 * X4 + X5 + X6 + 0.296 * 0.073 * 0.043 * X7 + X8 - X9 0.588 * 7 + 0.01 * X10 + 0, X 37 * 1 0.3 1 1 1 * X12 + 0.319 * H13- 19, 1 88, where XI - form oval cells (%);

X2 - form elongated cells (%);

HZ- entire cell membrane (%);

X4- membrane exfoliated cells (%);

X5- cell membrane is destroyed (%);

X6 - the cell membrane is sealed (%);

H7- oval shape of the cell nucleus (%);

X8 - rounded shape of the cell nucleus (%);

H9- entire membrane cell nucleus (%);

X 10 - gray color of the cell nucleus (%);

XI 1 - granular color of the cell nucleus (%);

XI 2 - nuclei character motion in the norm (%);

XI 3 - nuclei low amplitude motion (%)

build territorial map on which determine the presence of human tumor (benign or malignant), or its absence.

However, this method has the following disadvantages:

- does not allow to identify the localization of benign and malignant tumors

- does not allow to identify related oncological diseases. Known method [4], wherein the average established on the basis of the age of the curve and the nature of electronegative nuclei motion human buccal epithelial nuclei at microelectrophoresis can detect the presence onkoprotsessa in the body indicate the nature onkoprotsessa and related diseases (acute or chronic). This method is also used to follow the dynamics of the disease (prediction), but does not allow to identify the localization of benign and malignant tumors and localization comorbidities.

The closest in its functional purpose and essential features of the proposed method is a method of determining diagnosis of cytomorphological and tsitobiofizicheskim parameters buccal epithelial cells [3], which set reference samples allows to detect abnormalities in the organs and body systems, including the presence of malignant tumors with space finding this tumor. This technique is performed as follows:

1. The patient sample is taken buccal epithelial cells and conduct microelectrophoresis cells.

2. In determining the percentage of cytomorphological tsitobiofizicheskih parameters and buccal epithelial cells using the standards of these parameters.

3. Install for each parameter tsitobiofizicheskogo cytomorphological and buccal epithelial cells percentage.

4. Based on the percentages indicators determine the state of the human body.

However, the known method does not take into account important parameters such as specific cluster of cells and the condition of the sample, which can be more accurately identify benign and malignant tumors.

The object of the invention is to improve the accuracy of diagnosis of tumors express benign and malignant tumors and related diseases and for cytomorphological tsitobiofizicheskim parameters buccal epithelial cells.

Technical specification method is to increase the accuracy of rapid diagnosis of tumors, increasing the sensitivity of diagnosis of benign and malignant tumors, to achieve a high probability of detection process of benign and malignant tumors in a patient's body as in the early stages of the disease, as well as the later, a significant simplification of the preparation in the patient a certain time frame for collecting and storing diagnostic material, no infection "through rov "during the diagnostic sampling of the material, as well as the opportunity to fence diagnostic material in patients with poor venous filling, including the elderly and children.

The basis of the technical solutions which declares set task improvement process rapid diagnosis of benign and malignant tumors and related diseases according to cytomorphological and tsitobiofizicheskim parameters buccal epithelial cells which comprises mikroelektroforeznoe study in which on the basis of the established average age curve electronegative nuclei and character motion nuclei buccal and based on cytomorphologic indices sample bukkal nogo epithelium such as: the shape of the nucleus, the membrane of the nucleus, color nucleus form cells, membrane color cell cytoplasm, inclusion in the cytoplasm of the cell, the sample, the specific accumulation of cells is carried out the identification of benign and malignant tumors in a patient in the early and \ or the later stages of the disease, in which due to the fact that:

- figure Cytomorphological buccal form nuclei includes the following shape characteristics: oval, round, oblong, spindle-shaped, indeterminate;

- figure Cytomorphological buccal membrane nucleus membrane comprises the following characteristics: contoured, stratified, destroyed, sealed, rozpushenna;

- figure Cytomorphological buccal color kernel includes the following color characteristics: bright, dark, granular, dark to zerkalnosgi;

- figure Cytomorphological buccal epithelial cells form includes the following shape characteristics: oval, elongated, rounded, undefined, spindle-shaped;

- figure Cytomorphological buccal epithelial cells membrane comprises the following characteristics of the membrane: contoured, stratified, destroyed, sealed, rozpushenna,

- Cytomorphological indicator buccal color cell cytoplasm includes the following features color: light, dark, transparent, parchment;

- figure Cytomorphological buccal inclusion in the cytoplasm inclusions includes the following features: point, large granules, fine granules, granules dark, bright beads, bubbles; - figure Cytomorphological buccal sample comprising the sample characteristics: pollution rare biomaterial biomaterial severe pollution, sterile;

- figure Cytomorphological buccal epithelial cell clusters comprises clusters following characteristics: in the group; a track; in curls.

Furthermore, according to a method which is claimed, the characteristics of the nuclear shape is determined by the presence of: cancer stage 3-4 of the central nervous system (brain) and \ or malignancy stage 3-4 of the endocrine system (thyroid, pancreas) and \ or malignancy 3-4 steel and reproductive system (the female uterine, cervical, ovarian, cervical, uterine appendages, the external genital organs; men: prostate, testicles, scrotum, external genital of Organ Precursor Cells) and \ or malignancy 2-3 and 3-4 stages of kidney and urinary tract (ureters, urethra, bladder, urinary channel) and \ or malignancy 2-3 and 3-4 stages of the cardiovascular system (heart, pericardium, large and small blood vessels) and \ or malignancy 2-3 stages of the respiratory system (nasopharynx, trachea, bronchi, and lungs) and \ or malignancy stages 2-3 and 3-4 of the gastrointestinal tract (esophagus, stomach, and intestine) and \ or gepatoze and \ or zlokach ety neoplasm 2-3 and steps 3-4 liver and gallbladder (liver, bile duct and gall bladder) at a ratio of rounded core 10 to 59%, and stage 4 cancer of the central nervous system and \ or malignancy stage 4 Endocrine and \ or a stage 4 cancer of the reproductive system and \ or 4 renal cancer stage and urinary system and \ or malignancy stage 4 of the cardiovascular system and \ malignant shga 4 th step neoplasms of the respiratory system and \ or malignancy stage 4 of the gastrointestinal tract and \ or malignancy stage 4 liver and gallbladder at a ratio rounded core 60 to 100% and malignancy stage 1 -2 central nervous system, benign neoplasms with hyper function and \ or malignancy stage 1 -2 endocrine and \ or malignancy stage 1 -2 reproductive system and \ or benign Novo mations kidney and \ or malignancy 1 -2 and 2-3 stages and kidney urinary system and \ or malignancy 1 -2 and 2-3 stages of the cardiovascular system and \ or malignancy 1-2 and 2-3 respiratory steps system and \ or benign neoplasms of the bowel and malignancy stages 1-2 and 2-3 of the gastrointestinal tract and \ or malignancy 1-2 and steps 2-3 liver and gallbladder at a ratio of nucleus elongated from 10 to 59% and benign new rofessional central nervous system and \ or benign tumors of the reproductive system and \ or benign tumors of the kidneys and the urinary system, a benign neoplasm of the cardiovascular system and \ or benign neoplasms of the respiratory system and \ or benign neoplasms gastroduodenal gastrointestinal tract and \ or benign volumetric neoplasms of the liver and gallbladder in the ratio elongated core 60 to 100% and benign th and malignancy stages 1-2 and 2-3 of the central nervous system and \ or malignancy 1 -2 and 2-3 stages of the endocrine system and \ or benign and malignant neoplasms 1-2 and 2-3 of the reproductive system stages and \ or benign and malignant neoplasms 1 -2 and 2-3 kidney stages and lower parts of the urinary system and \ or benign and malignant neoplasms steps 1-2 and 2-3 of the cardiovascular system and \ or benign neoplasms of the respiratory system and \ and whether benign tumors and malignant neoplasms of the bowel 1 -2 and 2-3 stages of the gastrointestinal tract and \ or gepatoza liver, benign and malignant neoplasms volumetric 1-2 stages of the liver and gallbladder in the ratio of the spindle-shaped cores 10 to 39% and malignancy stages 3-4 and 4 of the central nervous system and \ or malignancy stages 3-4 and 4 endocrine system and \ or malignancy stage 3-4 reproductive system malignant new oobrazovaniya 3-4 and steps 4 and kidney urinary system and \ or malignancy 3-4 and 4 steps of the gastrointestinal tract and \ or malignancy 3-4 and 4 stages of liver and gall bladder, at a ratio of indefinite shape core 10 to 39%.

Details of the nuclear membrane is set by the presence of: - malignancy stages 2-3 and 3-4 of the central nervous system and \ or benign neoplasms at hypo-function and malignancy stages 2-3 and 3-4 of the endocrine system and \ or malignancy 2 -3 and 3-4 stages of the reproductive system and \ or malignant neoplasms U 2015/000039 2-3 ​​and 3-4 stages of kidney and urinary system and \ or malignancy 2-3 and 3-4 stages of the cardiovascular system and \ or malignant neoplasms step 3-4 the respiratory system and \ or benign tumors and malignant neoplasms of the bowel step 3-4 the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 2-3, 3-4 liver gallbladder stages and with a ratio of a compacted core of the membrane 10 to 59%, and benign and malignant neoplasms stage 1 -2 central nervous system and \ or benign new formation at hyper function and malignancy stage 1 -2 Endocrine and \ or benign and malignant neoplasms stage 1 -2 reproductive system and \ or benign and malignant neoplasms 1 -2 kidney and urinary system step and \ or benign and malignant neoplasms 1 - step 2 cardiovascular and \ or benign and malignant neoplasms 1 -2 and 2-3 respiratory phases and \ or benign neoplasms gastroduode cial zone and malignancy 1 -2 and 2-3 stages of the gastrointestinal tract and \ or malignancy step 1-2 liver and gallbladder at a ratio of compacted nucleus membrane of 60 to 100%, and benign and malignant tumors and 2 1 -2 -3 stages central nervous system and \ or benign neoplasms at hypo-function and malignancy 1 -2 and 2-3 endocrine stages and \ or malignancy 1 -2, 2-3, 3-4 stages of the reproductive system, and \ or benign neoplasms of the kidney and malignant neoplasms 1 -2, 2-3, 3-4 stages and kidney urinary system and \ or malignancy 1 -2, 2-3, 3-4 stages of the cardiovascular system and \ or benign and malignant tumors 1-2, 2-3, 3-4 respiratory phases and \ or benign tumors and malignant neoplasms of the bowel 1-2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3, 3-4 liver stages gallbladder at a ratio of exfoliated membranes from the nucleus 10 to 59% and malignancy stages 3-4 and 4 of the central nervous system malignancy and 4 stages 3-4 Endocrine and \ or malignancy 4 stages of the reproductive system and \ or malignancy 4 stage renal and urinary system and \ or cancer stage 4 of the cardiovascular system and \ or cancer stage 4 of the gastrointestinal tract and \ or benign but voobrazovaniya hepatosis and liver cancer stage 4 liver and gallbladder at a ratio of exfoliated membranes from the nucleus 60 to 100% and

malignancy 1 -2, 2-3, 3-4, and 4 steps of the central nervous system and \ or malignancy 1 -2, 2-3, 3-4, and 4 with the endocrine system tadiya and \ or malignancy 1 -2, 2-3, 3-4, and 4 stages of the reproductive system and \ or malignancy 1 -2, 2-3, and 4 of the kidneys and urinary system stages and \ or malignancy step 3-4 the cardiovascular system and \ or malignancy 2 -3 and 3-4 stages of the gastrointestinal tract at a ratio of fluff membrane core of 10 to 59% and zlok tive neoplasm 1 -2, 2-3, 3-4, and 4 steps of the central nervous system and \ or malignancy 1 -2, 2-3, 3-4, and 4 Endocrine stages and \ or malignancy 1 -2, 3 -4 and 4 stages of the reproductive system and \ or malignancy 1 -2, 3-4, and 4 stages of renal and urinary tract and \ or malignancy 1 -2, 2-3, 3-4, and 4 steps of the cardiovascular system and \ or benign and malignant neoplasms stage 1 -2 respiratory system cancer 1 -2, 2-3, 3-4, and 4 stages of the gastrointestinal tract and \ or benign neoplasms surround (cysts) and malignant neoplasms 2-3, 3-4, and 4 stages of the liver and gallbladder in the ratio of the destroyed nucleus membrane from 10 to 59%.

Characteristics kernel color is set by the presence of: cancer 1 -2 and 2-3 stages of the central nervous system and \ or benign neoplasms at hypo and \ or functions and the hyper malignancy 1 -2 and 2-3 Endocrine stages and \ or malignancy 1 -2 and 2-3 stages of the reproductive system and \ or cancerous neoplasms Nogo 1 -2 and 2-3 stages and kidney urinary system and \ or benign and malignant neoplasms 1 -2 and 2-3 stages cardiovascular systems s and \ or benign and malignant neoplasms 1 -2 and 2-3 respiratory phases and \ or good bone neoplasms and gastroduodenal bowel cancer and 1 -2 and 2-3 stages of the gastrointestinal tract and \ or benign liver neoplasm gepatoza and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3 stages of liver and gallbladder at a ratio of particulate core color of from 10 to 59% and malignancy stages 3-4 and 4 tse central nervous system and \ or malignancy stages 3-4 and 4 endocrine system and \ or malignancy 3-4 and 4 stages of the reproductive system and \ or malignancy 3-4 and steps 4 and kidney urinary system, cancer 3-4 4 and steps cardiovascular and \ or malignancy 3-4 and 4 stages of the respiratory system and \ or malignancy 3-4 and 4 steps of the gastrointestinal tract and \ or malignancy 3 and 4 -4 liver stages and gallbladder at a ratio of particulate core color of from 60 to 100% and malignancy 1 -2, 2-3, 3-4 stages of the central nervous system and \ or malignancy 1 -2, 2-3, 3-4 Endocrine steps and \ or malignancy 1 -2, 2-3, 3-4 stages of the reproductive system and \ or benign tumors and malignant tumors of kidney 1 -2, 2-3, 3-4 stages and kidney urinary system and \ or malignancy 1 -2 and 2-3 stages of the cardiovascular system and \ or benign and evil Qualitative neoplasms 1 -2 and 2-3 respiratory phases and \ or benign neoplasms and gastroduodenal malignancy 1 -2 and 2-3 stages of the gastrointestinal tract and \ or malignancy 1-2, 2-3 and 3-4 liver gallbladder stages and at a ratio of dark color of core 10 to 59%, and benign neoplasms of the central nervous system and \ or benign neoplasms at hypo and \ or hyper endocrine function and \ or benign neoplasms polo th system and \ or benign tumors of the lower parts of the urinary system and \ or benign tumors of the cardiovascular system and \ or benign tumors of the respiratory system and \ or benign neoplasms intestine and \ or benign neoplasms gepatoza liver and benign volumetric growths (cysts) of the liver and gall bubble nucleus at the ratio of dark color of from 60 to 100%, and benign and malignant neoplasms center stage 1-2 noy nervous system and \ or benign neoplasms at hypo and \ or functions and the hyper malignancy steps 1 -2 Endocrine and \ or benign and malignant neoplasms stage 1 -2 reproductive system and \ or benign and malignant neoplasm of kidney stage 1 -2 and urinary tract and \ or malignancy stage 1 -2 cardiovascular and \ or malignancy stage 1 -2 respiratory system and \ or benign neogenic Ia and gastroduodenal 1 -2 malignancy of gastrointestinal tract and \ or malignancy 1-2 stages of the liver and gallbladder in the dark to a ratio of specular color of the core 10 to 39%.

Cell shape characteristics set by the presence of: 1 malignancy -2, 2-3, 3-4 stages central nervous and \ or benign neoplasms at hypo-function and malignancy 1 -2, 2-3 and 3-4 Endocrine steps and \ or malignancy 1-2, 2-3, 3-4, and 4 stages of the reproductive system and \ or benign tumors and malignant tumors of kidney 1 -2, 2-3, 3-4 stages and kidney urinary system and \ or malignant neoplasms 1-2, 2-3, 3-4 stages of heart with udistoy system and \ or benign and malignant neoplasms 1-2 and 2-3 respiratory phases and \ or benign tumors and malignant neoplasms of the bowel 1 -2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign neoplasms surround (cyst) and malignant neoplasms 1-2, 2-3, 3-4, and 4 stages of liver and gallbladder at a ratio of rounded cells from 10 to 59%, and - a benign neoplasm of the central nervous system and \ or benign neoplasms n and hyper function, and cancer stage 1 -2 Endocrine and \ or benign tumors of the reproductive system and \ or benign tumors of the lower parts of the urinary system and \ or benign tumors of the cardiovascular system and \ or cancer stage 3-4 of the respiratory system and \ or benign neoplasms gastroduodenal and \ or benign tumors of the liver at the ratio of hepatosis rounded cells from 60 to 100% and - benign and malignant neoplasms 1 -2, 2-3, 3-4 stages of the central nervous system and \ or benign neoplasms at hypo-function and malignancy 1-2, 2-3 and 3-4 of the endocrine system steps and \ or benign and malignancy 1-2, 2-3 and 3-4 of the reproductive system of stages and \ or benign tumors of the lower parts of the urinary system and malignant neoplasm of 1-2, 2-3 and 3-4 stages of kidney and urinary system and \ or benign and malignantly neoplasms of 1-2, 2-3 and 3-4 stages of the cardiovascular system and \ or malignancy 1 -2, 2-3 and 3-4 respiratory phases and \ or benign and malignant neoplasms 1-2, 2- 3, the steps 3-4 of the gastrointestinal tract and \ or 0039 benign liver neoplasm hepatosis and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3, 3-4 stages of liver and gallbladder at a ratio elongated cells from 10 to 59% and benign tumors of the kidneys and \ or d brokachestvennogo neoplasms of the respiratory system with a ratio of cells that form vytyan from 60 to 100% and malignancy 1 -2, 2-3, 3-4 stages of the central nervous system and \ or malignancy 2-3 and 3-4 Endocrine stages and \ or malignancy 1 -2 and 3-4 stages of the reproductive system, cancer 1 -2 and 3-4 stages and kidney urinary system and \ or malignancy stage 1 -2 cardiovascular and \ or malignancy 1 and -2 2-3 Stud minutes gastrointestinal tract and \ or malignancy 1 -2 and 2-3 stages of liver and gallbladder at a ratio of indefinite form cells from 10 to 39%.

Characteristics of the cell membrane due to set prisuts ns: malignancy 1 -2 and 2-3 stages of the central nervous system and \ PLD. benign neoplasms at hypo and \ or functions and the hyper malignancy 1 -2 and 2-3 Endocrine stages and \ or malignancy 1 -2 and 2-3 reproductive system stages and \ or malignancy 1 -2 and 2-3 kidney and urinary system stages and \ or benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the cardiovascular system and \ or benign and malignant neoplasms 1 -2 and 2-3 respiratory phases and \ or benign and malignant ovoobrazovaniya 1 -2, 2-3 and 3-4 stages of the gastrointestinal tract at a ratio of exfoliated cells from the membrane 10 to 59% and malignancy stages 3-4 and 4 of the central nervous system and \ or malignancy stage 4 and the endocrine system \ or malignancy 4 stages of the reproductive system and \ or 4 renal cancer stage and urinary system and \ or malignancy stage 4 of the cardiovascular system and \ or malignancy 3-4 and steps 4 dyhat Yelnia system and \ or malignancy stage 4 of the gastrointestinal tract and \ or malignancy stage 4 liver and gall bladder and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2 2-3 3-4 and steps liver and gallbladder at a ratio of exfoliated cells from the membrane 60 and up to 100% - of benign tumors in hypo and \ or hyper endocrine function and \ or malignancy I have 2-3 stages of the reproductive system and \ or malignancy 2-3 stages of the cardiovascular system and \ or cancer stage 3-4 of the gastrointestinal tract at a ratio rospushennoy cell membranes from 10 to 39%, and cancer stage 2-3 endocrine and \ or benign and malignant neoplasms 1 -2 and 2-3 stages and kidney urinary system and \ or malignancy stage 3-4 of the cardiovascular system and \ or malignancy same step 3-4 ludochno tract at a ratio destroyed 10 to 59% of the cell membrane, and - 3-4 malignancy stage kidney and urinary system at a ratio of damaged cell membranes from 60 to 100%, and benign and malignant neoplasms 1 -2, 2-3, 3 -4 and 4 steps of the central nervous system and \ or with benign tumors and hyper function malignancy 1 -2 and 2-3 stages of the endocrine system and \ or benign and malignant neoplasms 1 -2 and 2-3 stages of the reproductive system and \ or malignancy stage 1 -2 cardiovascular and \ or malignancy 1-2, 2-3, 3-4 respiratory phases and \ or benign neoplasms and gastroduodenal malignancy stage 1 -2 gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and 1 -2 malignancy stage liver and gallbladder at a ratio of compacted cells from the membrane 10 to 59% and of okachestvennogo neoplasms stage 3-4 Endocrine and \ or malignancy 3-4 stage of the reproductive system and \ or benign and malignant lesions of 2-3 and 3-4 stages of the cardiovascular system and \ or benign tumors of the respiratory system and \ or benign neoplasms bowel cancer and 2-3, steps 3-4 of the gastrointestinal tract and \ or malignancy 2-3 and steps 3-4 liver and gallbladder at a ratio densified membrane Cl etki from 60 to 100%.

Characteristics of the cell cytoplasm color set by the presence: - of benign and malignant neoplasms 1 -2 and 4 steps of the central nervous system and \ or with benign tumors and hyper function malignancy 1-2, 2-3 Endocrine stages and \ or benign and malignant neoplasms 1 -2, 2-3, 3-4, and 4 stages of the reproductive system and \ or benign and malignant neoplasms 1-2, 2-3 and 3-4 and steps kidney urinary system and \ or malignancy NEW mation of 3-4 and 4 stages of the cardiovascular system and \ or malignancy 3-4 and 4 stages of the respiratory system and \ or malignancy 3-4 and 4 stages of the gastrointestinal tract and \ or benign neoplasms gepatoza liver cancer 2 3, 3-4 and 4 stages of liver and gallbladder in the transparent color cytoplasm ratio of 10 to 59% and malignancy stages 2-3 and 3-4 of the central nervous system and \ or malignancy stage 3-4 endocrine When transparent color cytoplasm ratio of 60 to 100% and malignancy 1 -2, 2-3, 3-4 stages of the central nervous system and \ or with benign tumors and hyper function malignancy 1-2, 2-3, 3- 4 stages endocrine and \ or malignancy 1-2, 2-3, 3-4 stages of the reproductive system and \ or malignancy 1-2, 2-3 and 3-4 and steps kidney urinary system and \ or malignancy 1 -2, 2-3, 3-4 stages of the cardiovascular system and \ or malignant but oobrazovaniya 1 -2, 2-3 and 3-4 respiratory phases and \ or malignancy 1 -2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign neoplasms gepatoza liver cancer 1 and 2, 2-3 and steps 3-4 liver and gallbladder in the ratio of dark color of the cytoplasm from 10 to 59%, and benign neoplasms of the central nervous system and \ or benign neoplasms of the endocrine system in hypo-function and \ or benign tumors of the reproductive system and \ or dobrokach ety neoplasm of kidney and urinary tract and \ or benign neoplasms of the cardiovascular system and \ or benign neoplasms of the respiratory system and \ or benign neoplasms of the gastrointestinal tract and \ or benign neoplasms surround (cysts) of the liver and gallbladder in the ratio of dark color of the cytoplasm of 60 to 100%, and stage 4 cancer of the central nervous system and \ or malignancy stage 4 endocrine system emy and \ or malignancy 4 stages of the reproductive system and \ or 4 renal cancer stage and urinary system and \ or malignancy stage 4 cardiovascular and \ or malignancy 4 stages of the respiratory system and \ or malignancy stage 4 zheludochno tract and \ or malignancy stage 4 liver and gallbladder in the cytoplasm ratio parchment color of from 60 to 100%.

Characteristics of inclusions in the cytoplasm of cells is set by the presence of: - a benign neoplasm of the central nervous system and \ or benign neoplasms at hypo-function of the endocrine system and \ or benign and malignant neoplasms 1 -2 and 2-3 stages of the reproductive system and \ or benign and malignant 1-2 neoplasm renal and urinary system step and \ or benign and malignant neoplasms step 1-2 the cardiovascular system and \ or dobrokaches Twain and malignancy stages 1-2 and 2-3 of the respiratory system and \ or benign and malignant neoplasms 1 -2 and 2-3 stages of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and 1 -2 malignancy stage liver and gallbladder at a ratio of inclusions in the cytoplasm of large, light fine granules of from 10 to 59% and benign tumors with hyper function and malignant neogenic I 1 -2, 2-3, 3-4 stages of the endocrine system and \ or malignancy 1 -2, 2-3, 3-4 stages of the central nervous system and \ or with a ratio of inclusions in the cytoplasm of large, small bright granules 60 to 100%, and benign and malignant neoplasms 1 -2 and 2-3 stages of the central nervous system and \ or benign neoplasms with hyper functions and stages of cancer of the endocrine system 1-2 and \ or benign and malignant neoplasms 1 -2 and 2-3 stages of the reproductive system and \ or dobrokaches Twain neoplasm of kidney and urinary tract and \ or benign and malignant neoplasms steps 2-3 and 3-4 of the cardiovascular system and \ or benign and malignant neoplasms 1 -2 and 3-4 stages of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1-2, 2-3, 3-4 from 10 to 59% of the liver and gallbladder steps at a ratio of inclusions in the cytoplasm of large, small and dark pellets benign neoplasms of the endocrine system in hypo-function and \ or malignancy step 3-4 the reproductive system and \ or malignancy 1-2, 2-3, 3-4, and 4 of the kidneys and urinary system stages and \ or malignancy 1 -2 step cardiovascular and \ or benign and malignant neoplasms 1 -2, 2-3, 3-4, and 4 stages of the respiratory system and \ or malignancy 2-3 and 4 steps of the gastrointestinal tract at a ratio of inclusions in the cytoplasm of large, IU lkih dark granules of from 60 to 100% and - benign neoplasms of the central nervous system and \ or benign tumors of the reproductive system and \ or benign neoplasms gastroduodenal and \ or benign liver neoplasm gepatoza at a ratio of inclusions in the cytoplasm of bubbles from 10 to 59%.

Characteristics of the sample is set by the presence of: benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the central nervous system and \ or benign neoplasms hypo-, hyper- function and malignancy 1-2, 2-3 and 3 4 stages endocrine and \ or benign and malignant neoplasms 1 -2, 2-3 and 3-4 of the reproductive system stages and \ or benign and malignant tumors

1 - 2, 2-3, 3-4 kidneys and urinary system stages, benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the cardiovascular system and \ or benign and malignant neoplasms 1 -2, 2- 3, the steps 3-4 of the respiratory system and \ or benign and malignant neoplasms 1 -2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1-2, 2-3 and 3-4 and liver stages zhelchnog Bubble in the presence of strongly contaminated biomaterial sample and stage 4 cancer of the central nervous system, and 'whether malignant neoplasms of the endocrine system 4 stages and \ or malignancy 4 stages of the reproductive system and \ or malignancy stage 4 of the cardiovascular system, malignancy stage 4 gastro -kishechnogo tract and \ or malignancy stage 4 liver and gallbladder in the presence of a sterile sample.

Characteristics cell clusters set by the presence: - of benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the central nervous system and \ or benign neoplasms at hypo and \ or functions and the hyper malignancy 1-2, 2- 3 and the steps 3-4 of the endocrine system and \ or benign and malignant neoplasms 1 -2,

2- 3 and 3-4 stages of the reproductive system and \ or benign and malignant neoplasms 1-2, 2-3, 3-4 stages and kidney urinary system and \ or benign and malignant neoplasms 1 -2, 2-3 and 3- 4 stages of the cardiovascular system and \ or benign and malignant lesions of 1-2, 2-3, 3-4 respiratory phases and \ or benign and malignant lesions of 1-2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign tumors of the liver gepatoza and \ or benign volumetric of neoplasm (cysts) and malignant neoplasms 1 -2, 2-3 and 3-4 stages of liver and gallbladder in the presence of congestion in the group and malignancy stages 2-3 and 3-4 of the central nervous system and \ or malignancy 1- 2, 2-3, 3-4 stages of the endocrine system and \ or benign and malignant neoplasms 1-2, 2-3 and 3 of 4 stages of the reproductive system and \ or benign and malignant neoplasms 1-2, 2-3, 3-4 kidney and urinary system of stages and \ or malignancy 1 -2, 2-3, 3-4 with tadiya cardiovascular and \ or malignancy 1-2, 2-3, 3-4 respiratory phases and \ or benign neoplasms and gastroduodenal malignancy 1 -2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3 and 3-4 stages of liver and gallbladder in the presence of clusters in tracks and malignant neoplasm of the central stage 3-4 nervous system and \ or malignancy 2-3 and 3-4 Endocrine stages and \ or malignancy 2-3 and 3-4 of the reproductive system stages and \ or malignancy 1-2, 2-3, 3-4 kidney steps and urinary system and \ or malignancy 2-3 and 3-4 stages of the cardiovascular system and \ or cancer stage 3-4 of the respiratory system and \ or malignancy 2-3 and 3-4 stages of the gastrointestinal tract and \ or malignancy 1 -2, 2-3 and 3-4 Pec steps and none of the gallbladder in the presence of clusters in curls.

Inventive step.

Due to the fact that in the proposed method for rapid diagnosis of benign and malignant tumors and related diseases are considered not only cytomorphological and tsitobiofizicheskie parameters buccal epithelial cells, but also parameters such as a specific cluster of cells and state of the sample, the proposed method allows to improve the accuracy and sensitivity diagnosis of benign and malignant neoplasms.

Said technical result is achieved as follows. Buccal sample with the inner surface of the cheeks (middle layer) are dissolved drop phosphate buffer (with Ph = 7,4) of the syringe (pipette), a spatula is placed between two cover glasses are placed in a chamber and electrodes for microelectrophoresis carry electrical contact. The cell sample is placed under the lens of the microscope, after which include microelectrophoresis device and observe the movement of the nuclei in the native cells while increasing from 100 to 400 times. Moving toward the anode nuclei indicates that they have a negative electrical charge.

Electrophoresis was carried out in a mode which allows cells sufficiently long pot life (15 V / cm, the amperage of 100 mA). In this case, fix the cells to the active and inactive cores.

Further, a method which is claimed, is as follows:

- assess cytomorphologic indices buccal sample, determining the percentage of these indicators buccal epithelial cells:

nuclear shape: oval, round, oblong, spindle-shaped, undefined;

- the membrane of the nucleus: contoured, layered, broken seals, fluff;

- color kernel: bright, dark, granular, dark mirror;

- the form of cells: oval, oblong, round, uncertain, spindle-shaped;

- the cell membrane: contoured, layered, broken seals, fluff;

- color of the cell cytoplasm: light, dark, transparent, parchment;

- inclusion in the cytoplasm of the cell: point, large granules, fine granules, granules dark, bright beads, bubbles;

Sample: rare biomaterial contamination, heavy contamination biomaterial, sterile;

- a specific cluster of cells: in the group; a track; in curls. Technical result confirmed by the following method parameters buccal cell parameters:

norm:

form oval nucleus;

contoured nucleus membrane; kernel color light;

form oval cells;

the cell membrane is contoured;

color cell cytoplasm light;

inclusion in the cytoplasm point;

in a rare sample contamination biomaterial;

No specific clusters of cells.

All other parameters cytomorphological buccal epithelial cells is a deviation from the norm.

If there onkoprotsessa in the human body, buccal epithelium has cytomorphological and tsitobiofizicheskie options that deviate from the norm. But in practice there are situations in which, in the presence of parameters indicative of oncological causes, there are also options buccal epithelial cells (small amounts of percentages) are normal.

For the diagnosis of tumors are important parameter changes buccal epithelial cell nuclei and their percentage ratio: nuclear shape, color kernel, the kernel integrity of the membrane, the presence and activity of the movement and the amplitude oscillations of the core during the intracellular microelectrophoresis.

An important technical feature of the process is also the percentage of the cell parameters (in particular - the inclusion in the cytoplasm in the form of beads, bubbles, color of the cytoplasm, and then changes the cell membrane and cell shape).

For the diagnostic to identify tumor processes, and their localization step rassmotreEshe necessary the whole sample, and determining the presence of specific concentrations of buccal epithelial cells and clusters of specific species.

The analysis of one sample takes approximately 5 to 10 minutes.

Experimental studies of the method rapid diagnosis of benign and malignant tumors and related diseases and for cytomorphological tsitobiofizicheskim parameters buccal epithelial cells in a clinical setting have shown its high efficiency. Method rapid diagnosis in its use achieves improve accuracy diagnosis of tumors on the day the patient's circulation, a significant increase in the differential diagnosis of sensitivity of benign and malignant tumors, to achieve a high probability of detection process of benign and malignant tumors in a patient's body both at early stages of disease, and more later.

Examples of the method.

Implementation of the proposed method for rapid diagnosis of benign and malignant tumors and related diseases and for cytomorphological tsitobiofizicheskim parameters buccal epithelial cells are illustrated by the following clinical examples.

Example 1. According to the proposed method, the patient Ν »1 was diagnosed with cancer of the thyroid gland in the mts with neck lymph nodes (Fig. 1/3). According to the results of the traditional survey of cancer of the larynx, mts has been found in the lymph nodes of the neck. Parameters buccal cells from the patient:

- core form: rounded 57%, undefined 21%, 7% elongated, spindle-shaped 8%, 7% oval.

- Diaphragm core: 67% layered, compacted January 1%, 12% fluff, destroyed

10%.

- Color core: 76% particulate, 24% dark.

- Inclusion in the cytoplasm of cells: beads blond 94%, 6% black granules.

- Color cell cytoplasm: 87% transparent, dark 1 3%.

- cells Shape: elongated 56%, round 26%, 18% uncertain.

- Cells Membranes: 93% compacted, destroyed 3%, 4% fluffing.

- The sample is heavily contaminated biomaterial.

- cluster of cells: in the group; a track; in curls.

Example 2. According to the proposed method 2 NQ patient was diagnosed with cancer ascending colon card with partial obstruction (Fig. 2/3). According to the results of the traditional survey was diagnosed with cancer of the colon ascending department I II Art., Compensated ileus. Parameters buccal cells from the patient:

- core form: rounded 58%, undefined 23%, 7% elongated, spindle-shaped 5%, 7% oval.

- Diaphragm core: 54% layered, thick 20% fluff 14% destroyed

12 %.

- Color core: Granular 96%, 4% black.

- Incorporation into the cytoplasm of cells: 58% dark granules, pellets light 8%), blisters 9%, 25% point. - Color cell cytoplasm: Dark 58%, 29% transparent, light 9%, 4% of the parchment.

- Cells form: 57% elongated, rounded 26%, undefined 8%, oval

9%.

- Cells Membranes: sealed 64%, 12% layered, January 1% fluff, 13% destroyed.

- The sample is heavily contaminated biomaterial.

- cluster of cells: in the group; a track; in curls.

Example 3. According to the proposed method, the patient has N ° 3 was detected with germination kidney cancer into soft tissue mts retroperitoneal lymph node (Fig. 3/3). According to the results of the traditional survey of cancer of the left kidney was discovered III century. Parameters buccal cells from the patient:

- core form: rounded 56%, undefined 22%, 8% elongated, spindle-shaped 6%, 8% oval.

- Diaphragm core: 53% dense sandwiched 23%, 24% destroyed.

- Color core: 82% particulate, 18% dark.

- Incorporation into the cytoplasm of cells: beads dark 94%, 6% dot.

- Color cell cytoplasm: Dark 47%, 48% transparent, light 5%.

- cells Shape: elongated 49%, rounded to 24%, 27% uncertain.

- Cells Membranes: 83% destroyed is sealed 8%, 9% fluff.

- The sample is heavily contaminated biomaterial.

- cluster of cells: in the group; a track; in curls. As seen from the description "Method" it differs significantly from the prototype, and is consequently new.

INDUSTRIAL APPLICABILITY "Method" which is claimed is reflected in the examples and in the particular embodiment of Figure 1 -3.

Practical use of "method", which is non-traumatic, nsinvazivnym and does not require sophisticated equipment and long-term survey will help to carry out a screening study with prophylactic examinations and dispensary observation at risk.

Information sources.

1. Patent RU 2527676 Bull jY ° 25, 2013

2. Patent UA 61495 Bul M> 14, 1 201 g

3. Patent UA 92715 Bull il 6, 2014

4. USSR Patent No. 1 169 614 M Bul ° 10, 1981 g.

Claims

Formula
1 . Method rapid diagnosis of benign and malignant tumors and related diseases according to cytomorphological and tsitobiofizicheskim parameters buccal epithelial cells, which includes mikroelektroforeznoe study in which on the basis of the established average age curve electronegative nuclei and the nature of the nuclei buccal, and based on cytomorphologic indices buccal sample: the shape of the nucleus, the membrane of the nucleus, the nucleus color, shape cells meme cell injury, color cell cytoplasm, inclusion in the cell cytoplasm, the sample, the specific accumulation of cells is carried out the identification of benign and malignant tumors in a patient in the early and \ or late stages of the disease, which is characterized in that:
- figure Cytomorphological buccal form nuclei includes the following shape characteristics: oval, round, oblong, spindle-shaped, undefined;
- figure Cytomorphological buccal membrane nucleus membrane comprises the following characteristics: contoured, stratified, destroyed, sealed, fluff;
- figure Cytomorphological buccal color kernel includes the following color characteristics: bright, dark, granular, dark mirror;
- figure Cytomorphological buccal epithelial cells form includes the following shape characteristics: oval, elongated, rounded, undefined, spindle-shaped;
- figure Cytomorphological buccal epithelial cells membrane comprises a membrane following characteristics: contoured, stratified, destroyed, sealed, fluff;
- Cytomorphological indicator buccal color cell cytoplasm includes the following features color: light, dark, transparent, parchment;
- figure Cytomorphological buccal inclusion in cytoplasm of cells comprises the following characteristics inclusions: point, large granules, fine granules, granules dark, bright beads, bubbles; - figure Cytomorphological buccal sample comprising the sample characteristics: pollution rare biomaterial biomaterial severe pollution, sterile;
- figure Cytomorphological buccal epithelial cells specific cluster includes clusters of the following characteristics: in the group; a track; in curls.
2. A method according to claim 1 characterized in that the mold core characteristics determined by the presence of:
- cancer stage 3-4 of the central nervous system (brain) and \ or cancer stage 3-4 of the endocrine system (thyroid, pancreas) and \ or malignancy 3-4 stage of the reproductive system (the female uterus, cervix, , ovarian, cervical, uterine appendages, vulva; M: prostate, testicles, scrotum, external genitalia) and \ or malignancy 2-3 and 3-4 and steps kidney mochevydslitelnoy system (ureter in, urethra, bladder, urinary channel) and \ or malignancy 2-3 and 3-4 stages of the cardiovascular system (heart, pericardium, large and small blood vessels) and \ or malignancy 2-3 stages of the respiratory system ( nasopharynx, trachea, bronchi and lungs) and \ or malignancy 2-3 and 3-4 stages of the gastrointestinal tract (esophagus, stomach and intestines) and \ or gepatoze and \ or malignancy 2-3 and 3-4 of the liver stages and gallbladder (liver, bile ducts and gall puzy I) at a ratio of rounded core 10 to 59% and
- malignancy stage 4 of the central nervous system and \ or malignancy stage 4 Endocrine and \ or malignancy 4 stages of the reproductive system and \ or malignancy 4 renal stage and urinary system and \ or malignancy stage 4 of the cardiovascular system and \ or cancer stage 4 of the respiratory system and \ or cancer stage 4 of the gastrointestinal tract and \ or malignant novoobr mations stage 4 liver and gallbladder in the rounded core ratio of 60 to 100% and
- malignancy step 1-2 the central nervous system, a benign neoplasm with hyper function and \ or malignancy stage 1 -2 Endocrine and \ or malignancy stage 1 -2 reproductive system and \ or benign neoplasm of kidney and \ or malignancy 1 2 and 2-3 stages and kidney urinary system and \ or malignancy 1 -2 and 2-3 stages of the cardiovascular system and \ or malignancy 1-2 and 2-3 respiratory steps Yelnia system and \ or benign neoplasms of the bowel and malignancy stages 1-2 and 2-3 of the gastrointestinal tract and \ or malignancy 1 -2 and 2-3 stages of liver and gallbladder at a ratio of nucleus elongated from 10 to 59% and
benign neoplasms of the central nervous system and \ or benign tumors of the reproductive system and \ or benign tumors of the kidneys and urinary tract, benign tumors of the cardiovascular system and \ or benign tumors of the respiratory system and \ or benign tumors of the gastroduodenal zone of the digestive tract and \ or benign surround liver and gall bladder neoplasm in a ratio of elongated core 60 to 100% and
- benign and malignant neoplasms steps 1-2 and 2-3 of the central nervous system and \ or malignancy 1 -2 and 2-3 stages of the endocrine system and \ or benign and malignant neoplasms 1 -2 and 2-3 stages of the reproductive system and \ or benign and malignant neoplasms and 1 -2 3 2- kidney stages and lower parts of the urinary system and \ or benign and malignant neoplasms steps 1-2 and 2-3 of the cardiovascular system and \ or benign neoplasms dyhat Yelnia system and \ or benign tumors and malignant neoplasms of the bowel 1 -2 and 2-3 stages of the gastrointestinal tract and \ or gepatoza liver, benign and malignant neoplasms volumetric 1-2 stage liver and gallbladder at a ratio of the spindle-shaped nuclei from 10 to 39% and
- malignancy stages 3-4 and 4 of the central nervous system and \ or malignancy 3-4 and 4 Endocrine stages and \ or malignancy stage 3-4 of the reproductive system, cancer of the kidneys 3-4 and steps 4 and urinary system and \ or malignancy 3-4 and 4 steps of the gastrointestinal tract and \ or malignancy 3-4 and 4 stages of liver and gall bladder, at a ratio of indefinite shape kernel from 10 to 39%.
3. A method according to claim 1 characterized in that the characteristics of the nucleus membrane is set by the presence of: - malignancy stages 2-3 and 3-4 of the central nervous system and \ or benign neoplasms at hypo-function and malignancy 2-3 and 3 -4 stages endocrine and \ or malignancy 2-3 and 3-4 stages of the reproductive system and \ or malignancy 2-3 and 3-4 stages of kidney and urinary system and \ or malignancy 2-3 and 3-4 stages the cardiovascular system we and \ or malignancy stage 3-4 of the respiratory system and \ or benign neoplasms intestine and cancer stage 3-4 of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 2- 3, steps 3-4 liver and gallbladder at a ratio of compacted nucleus membrane of 10 to 59% and
- benign and malignant neoplasms stage 1 -2 central nervous system and \ or with benign tumors and hyper function malignancy stage 1 -2 Endocrine and \ or benign and malignant neoplasms stage 1 -2 reproductive system and \ or benign and malignant neoplasms 1 -2-stage renal and urinary tract and \ or benign and malignant neoplasms stage 1 -2 cardiovascular and \ or benign and malignancy 1 -2 and 2-3 stages of the respiratory system and \ or benign neoplasms and gastroduodenal malignancy stages 1-2 and 2-3 of the gastrointestinal tract and \ or malignancy 1-2 stages of the liver and gallbladder in the ratio sealed diaphragm core 60 to 100% and
- benign and malignant neoplasms 1 -2 and 2-3 stages of the central nervous system and \ or benign neoplasms at hypo-function and malignancy stages 1-2 and 2-3 of the endocrine system and \ or malignancy 1 -2 2-3 3-4 stages of the reproductive system and \ or benign tumors and malignant tumors of kidney 1 -2, 2-3, 3-4 stages and kidney urinary system and \ or malignancy 1 -2, 2-3, 3-4 cardio steps -sosudistoy system and \ or benign th and malignancy 1-2, 2-3, 3-4 stages of the respiratory system and \ or benign neoplasms and bowel cancer 1 - 2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign neoplasms volume ( cysts) and malignant neoplasms 1 -2, 2-3, 3-4 stages of liver and gallbladder at a ratio of layered membrane nucleus of 10 to 59% and
- malignancy stages 3-4 and 4 of the central nervous system malignancy and 4 stages 3-4 Endocrine and \ or malignancy 4 stages of the reproductive system and \ or 4 renal cancer stage and urinary system and \ or malignancy stage 4 cardiovascular system and \ or cancer stage 4 of the gastrointestinal tract and \ or benign tumors of the liver and gepatoza malignancy 4 adii liver and gallbladder at a ratio of exfoliated membranes from the nucleus 60 to 100% and
- malignancy 1 -2, 2-3, 3-4, and 4 steps of the central nervous system and \ or malignancy 1-2, 2-3, 3-4, and 4 Endocrine stages and \ or malignancy 1 -2, 2-3, 3-4, and 4 stages of the reproductive system and \ or malignancy 1 -2, 2-3, and 4 of the kidneys and urinary system stages and \ or malignancy step 3-4 the cardiovascular system and \ or malignancy 2 -3 and 3-4 stages of the gastrointestinal tract at a ratio of 10 to 59% fluff core and membrane
- malignancy 1 -2, 2-3, 3-4, and 4 steps of the central nervous system and \ or malignancy 1 -2, 2-3, 3-4, and 4 stages Endocrine and \ or malignancy 1-2 3-4 and 4 stages of the reproductive system and \ or malignancy 1 -2, 3-4, and 4 stages of renal and urinary tract and \ or malignancy 1-2, 2-3, 3-4, and 4 stages cardiovascular system and \ or benign and malignant neoplasms stage 1 -2 respiratory system malignancy 1-2, 2-3, 3 -4 and 4 steps of the gastrointestinal tract and \ or benign neoplasms surround (cysts) and malignant neoplasms 2-3, 3-4, and 4 stages of the liver and gallbladder in the ratio of the destroyed nucleus membrane from 10 to 59%.
4. A method according to claim 1 characterized in that the color characteristics of the nucleus is set by the presence of:
- malignancy 1 -2 and 2-3 stages of the central nervous system and \ or benign neoplasms at hypo and \ or functions and the hyper malignancy stages 1-2 and 2-3 of the endocrine system and \ or malignancy 1-2 and 2 -3 reproductive system stages and \ or malignancy 1-2 and 2-3 and steps kidney urinary system and \ or benign and malignant neoplasms steps 1-2 and 2-3 of the cardiovascular system and \ or benign and malignant neoplasms 1 2 and 2-3 respiratory phases and \ or benign neoplasms gastroduodenal and intestinal cancer and 1 -2 and 2-3 stages of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignancy 1 -2, 2-3 stages of liver and gallbladder at a ratio of particulate core color of from 10 to 59% and
- malignancy stages 3-4 and 4 of the central nervous system and \ or malignancy stages 3-4 and 4 endocrine system and \ or malignancy 3-4 and 4 stages of the reproductive system and \ or malignancy 3-4 and 4 renal steps and urinary system, cancer of 3-4 and 4 stages of the cardiovascular system and \ or malignancy 3-4 and 4 stages of the respiratory system and \ or malignancy 3-4 and 4 stages of the gastrointestinal tract and \ or zlok Qualitative neoplasm 3-4 and 4 stages of liver and gallbladder in the granular core color ratio of 60 to 100% and
- malignancy 1 -2, 2-3, 3-4 stages of the central nervous system and \ or malignancy 1 -2, 2-3, 3-4 stages of the endocrine system and \ or malignancy 1 -2, 2-3, 3-4 stages of the reproductive system and \ or benign tumors and malignant tumors of kidney 1 -2, 2-3, 3-4 stages and kidney urinary system and \ or malignancy stages 1-2 and 2-3 of the cardiovascular system and \ and benign or malignant neoplasms of 1 -2 and 2-3 respiratory phases and \ and whether benign neoplasms and gastroduodenal malignancy 1 -2 and 2-3 stages of the gastrointestinal tract and \ or malignancy 1 -2, 2-3 and 3-4 stages of liver and gallbladder when coo elations dark color of core 10 to 59% and
benign neoplasms of the central nervous system and \ or benign neoplasms with hypo- and \ or hyper function of the endocrine system and \ or benign tumors of the reproductive system and \ or benign tumors of the lower parts of the urinary system and \ or benign tumors of the cardiovascular system and \ or benign neoplasms respiratory system and \ or benign tumors of the intestine and \ or benign neoplasms gepatoza Pec and volume and benign neoplasms (cysts) of the liver and gallbladder in the ratio of dark color of core 60 to 100% and
- benign and malignant neoplasms stage 1 -2 central nervous system and \ or benign neoplasms at hypo and \ or functions and the hyper malignancy of the endocrine system 1 -2 stages and \ or benign and malignant neoplasms stage 1 -2 reproductive system and \ or benign and malignant neoplasms 1 -2 kidney and urinary system step and \ or malignancy stage 1 -2 cardiovascular and \ or malignant start- Nia stage 1 -2 respiratory system and \ or benign neoplasms and gastroduodenal 1 -2 malignancy of gastrointestinal tract and \ or malignancy 1-2 stages of the liver and gallbladder in the dark to a ratio of specular color of the core 10 to 39%.
5. A method according to claim 1 characterized in that the cell shape characteristics set by the presence of:
- malignancy 1 -2, 2-3, 3-4 stages of the central nervous system and \ or benign neoplasms at hypo-function and malignancy 1 -2, 2-3 and 3-4 of the endocrine system steps and \ or malignancy 1 2, 2-3, 3-4, and 4 stages of the reproductive system and \ or benign tumors and malignant tumors of kidney 1 -2, 2-3, 3-4 stages and kidney urinary system and \ or malignancy 1 -2, 2 -3, 3-4 stages of the cardiovascular system and \ or benign and zlokachest venous neoplasm steps 1-2 and 2-3 of the respiratory system and \ or benign neoplasms and intestinal malignancy 1 -2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 2, 2-3, 3-4, and 4 stages of liver and gallbladder at a ratio of rounded cells from 10 to 59% and
benign neoplasms of the central nervous system and \ or with benign tumors and hyper function malignancy stage 1 -2 Endocrine and \ or benign tumors of the reproductive system and \ or benign neoplasms of the lower divisions of the urinary system and \ or benign neoplasms of the cardiovascular system and \ or cancer stage 3-4 of the respiratory system and \ or benign tumors of the gastroduodenal zone s and \ or benign tumors of the liver at the ratio of hepatosis rounded cells from 60 to 100% and
- benign and malignant neoplasms 1 -2, 2-3, 3-4 stages of the central nervous system and \ or benign neoplasms at hypo-function and malignancy 1 -2, 2-3 and 3-4 stages of the endocrine system and \ or benign and malignancy 1 -2, 2-3 and 3-4 of the reproductive system stages and \ or benign neoplasms of the lower divisions of the urinary system and malignancy 1 -2, 2-3 and 3-4 and steps kidney urinary system and \ or benign and malignant neoplasms of 1 -2, 2-3 and 3-4 stages of the cardiovascular system and \ or malignancy 1 -2, 2-3 and 3-4 respiratory phases and \ or benign and malignant neoplasms 1 -2, 2- 3, the steps 3-4 of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3, 3-4 stages of liver and gallbladder at a ratio of elongated cells from 10 to 59% and
- benign neoplasm of kidney and \ or benign neoplasms of the respiratory system with a ratio of cells elongated from 60 to 100% and
- malignancy 1 -2, 2-3, 3-4 stages of the central nervous system s and \ or malignancy 2-3 and 3-4 Endocrine stages and \ or malignancy 1 -2 and 3-4 stages of the reproductive system, malignancy 1 -2 and 3-4 kidney and urinary systems steps s and \ or malignancy stage 1 -2 cardiovascular and \ or malignancy 1 -2 and 2-3 stages of the gastrointestinal tract and \ or malignancy 1 -2 and 2-3 stages of liver and gall pU yrya indefinite shape with a ratio of cells 10 to 39%.
. 6. A process according to claim 1 characterized in that the characteristic cell membrane iki set by the presence of:
- malignancy 1 -2 and 2-3 stages of the central nervous system and \ or benign neoplasms at hypo and \ or functions and the hyper malignancy 1 -2 and 2-3 Endocrine stages and \ or malignancy 1 and -2 2-3 stages of the reproductive system malignant neoplasms Miles 1 -2 and 2-3 stages and kidney urinary system and \ or benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the cardiovascular system and \ or benign and malignant start-ups anija 1 -2 and 2-3 respiratory phases and \ or benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the gastrointestinal tract at a ratio of exfoliated cells from the membrane 10 to 59% and
- malignancy stages 3-4 and 4 of the central nervous system and \ or malignancy stage 4 Endocrine and \ or malignancy 4 stages of the reproductive system and \ or 4 renal cancer stage and urinary system and \ or malignancy stage 4 serdechno vascular system and \ or malignancy 3-4 and 4 stages of the respiratory system and \ or cancer stage 4 of the gastrointestinal tract and \ or malignant ovoobrazovaniya stage 4 liver and gall bladder and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3 and 3-4 stages of liver and gallbladder at a ratio of exfoliated cells from the membrane 60 to 100% and
- benign growths in hypo and \ or hyper function of the endocrine system and \ or malignancy 2-3 stage of the reproductive system and \ or malignancy 2-3 stages of the cardiovascular system and \ or cancer stage 3-4 of the gastrointestinal tract rospushennoy at a ratio of cell membranes from 10 to 39% and
cancer of the endocrine system step 2-3 and \ or benign and malignant neoplasms 1 -2 and 2-3 stages and kidney urinary system and \ or malignancy stage 3-4 of the cardiovascular system and \ or malignancy stage 3-4 gastro -kishechnogo tract at a ratio of damaged cell membranes from 10 to 59% and
- 3-4 malignancy stage and kidney urinary system at a ratio of damaged cell membranes from 60 to 100% and
- benign and malignant neoplasms 1 -2, 2-3, 3-4, and 4 steps of the central nervous system and \ or with benign tumors and hyper function malignancy 1-2 and 2-3 Endocrine stages and \ or benign and malignant tumors 1 -2 and 2-3 stages of the reproductive system and \ or malignancy stage 1 -2 cardiovascular and \ or malignancy 1 -2, 2-3, 3-4 stages of the respiratory system and \ or benign neoplasms gastroduodenal zone and malignancy stage 1 -2 gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and 1 -2 malignancy stage liver and gallbladder at a ratio of compacted cells from the membrane 10 to 59% and
cancer stage 3-4 Endocrine and \ or malignancy 3-4 stage of the reproductive system and \ or benign and malignant lesions of 2-3 and 3-4 stages of the cardiovascular system and \ or benign tumors of the respiratory system and \ or benign neoplasms bowel cancer and 2-3, steps 3-4 of the gastrointestinal tract and \ or malignancy 2-3 and steps 3-4 liver and gallbladder at a ratio densified membrane to etki from 60 to 100%.
7. A method according to claim 1 characterized in that the color characteristics of the cell cytoplasm is set by the presence of:
- benign and malignant neoplasms 1 -2 and 4 steps of the central nervous system and \ or with benign tumors and hyper function malignancy 1-2, 2-3 Endocrine stages and \ or benign and malignant neoplasms 1 -2, 2-3, 3-4 and 4 stages of the reproductive system and \ or benign and malignant neoplasms 1 -2, 2-3 and 3-4 and steps mochsvydelitelnoy renal system and \ or malignancy stages 3-4 and 4 cardiovascular and \ or malignant n ovoobrazovaniya 3-4 and 4 stages of the respiratory system and \ or malignancy 3-4 and 4 steps of the gastrointestinal tract and \ or benign tumors of the liver and gepatoza malignancy 2-3, 3-4, and 4 stages of liver and gallbladder in the ratio transparent color cytoplasm from 10 to 59% and
- malignancy stages 2-3 and 3-4 of the central nervous system and \ or malignancy stage 3-4 of the endocrine system, with a ratio of 60 to 100% of the cytoplasm and the transparent color
- malignancy 1-2, 2-3, 3-4 stages of the central nervous system and \ or with benign tumors and hyper function malignancy 1 -2, 2-3, 3-4 stages of the endocrine system and \ or malignancy 1 - 2, 2-3, 3-4 stages of the reproductive system and \ or malignancy 1 -2, 2-3 and 3-4 and steps kidney urinary system and \ or malignancy 1 -2, 2-3, 3-4 steps cardiovascular system and \ or malignancy 1 -2, 2-3 and 3-4 respiratory phases and \ or h okachestvennogo neoplasms 1 -2, 2-3, 3-4 stages of the gastrointestinal tract and \ or benign neoplasms hepatosis and liver cancer 1 -2, 2-3 and 3-4 stages of liver and gallbladder in the ratio of dark color of the cytoplasm of 10 to 59% and
benign neoplasms of the central nervous system and \ or benign tumors of the endocrine system in hypo-function and \ or benign tumors of the reproductive system and \ or dobrokachestvennog of kidney tumors and urinary system and \ or benign tumors of the cardiovascular system and \ or benign tumors of the respiratory system and \ or benign tumors of the gastrointestinal tract and \ or benign neoplasms volume (kitty thou) liver and gallbladder in the ratio of dark color of the cytoplasm from 60 to 100% and
- malignancy stage 4 of the central nervous system and \ or malignancy stage 4 Endocrine and \ or malignancy 4 stages of the reproductive system and \ or malignancy 4 renal stage and urinary system and \ or malignancy stage 4 of the cardiovascular system and \ or cancer stage 4 of the respiratory system and \ or cancer stage 4 of the gastrointestinal tract and \ or malignant novoobr mations stage 4 liver and gallbladder in parchment color cytoplasm ratio of 60 to 100%.
8. A method according to claim 1 characterized in that the characteristics of the inclusions in the cytoplasm of cells is set by the presence of:
benign neoplasms of the central nervous system and \ or benign neoplasms at hypo-function of the endocrine system and \ or benign and malignant neoplasms 1 -2 and 2-3 reproductive system stages and \ or benign and malignant neoplasms 1 -2 kidney and urinary system step and \ and benign or malignant neoplasms of 1-2 stages of the cardiovascular system and \ or benign and malignant neoplasms 1 -2 and 2-3 respiratory phases and \ and whether benign and malignant neoplasms 1 -2 and 2-3 stages of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and 1 -2 malignancy stage liver and gallbladder at a ratio of inclusions in the cytoplasm of large , small bright granules of from 10 to 59% and
- benign tumors with hyper function and malignancy 1 -2, 2-3, 3-4 Endocrine stages and \ or malignancy 1 -2, 2-3, 3-4 stages of the central nervous system and \ or with a ratio of inclusions in cytoplasm large, small bright granules of from 60 to 100% and
- benign and malignant neoplasms 1 -2 and 2-3 stages of the central nervous system and \ or with benign tumors and hyper function malignancy stage 1 -2 Endocrine and \ or benign and malignant neoplasms 1 -2 and 2-3 stages of the reproductive system and \ or benign tumors of the kidneys and urinary system, and \ or benign and malignant lesions of 2-3 and 3-4 stages of the cardiovascular system and \ or benign and zlokachestvennog neoplasms of steps 1-2 and 3-4 of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3, 3-4 stages of liver and gallbladder with ratio of inclusions in the cytoplasm of large, small dark granules of from 10 to 59% and
- benign growths in hypo-function of the endocrine system and \ or malignancy step 3-4 the reproductive system and \ or malignancy 1 -2, 2-3, 3-4, and 4 stages kidney and urinary tract and \ or malignancy 1- step 2 cardiovascular and \ or benign and malignant neoplasms 1 -2, 2-3, 3-4, and 4 stages of the respiratory system and \ or malignancy 2-3 and 4 steps of the gastrointestinal tract at a ratio of inclusions in the cytoplasm of large , m elkih dark granules of from 60 to 100% and
benign neoplasms of the central nervous system and \ or benign tumors of the reproductive system and \ or benign neoplasms gastroduodenal and \ or benign liver neoplasm gepatoza at a ratio of inclusions in the cytoplasm of bubbles from 10 to 59%.
9. A method according to claim 1 characterized in that the characteristics of the sample set by the presence of:
- benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the central nervous system and \ or benign neoplasms hypo-, hyper- function and malignancy 1 -2, 2-3 and 3-4 and steps Endocrine \ or benign and malignant lesions of 1-2, 2-3 and 3-4 of the reproductive system of stages and \ or benign and malignant lesions
1 - 2, 2-3, 3-4 kidneys and urinary system stages, benign and malignant neoplasms 1-2, 2-3 and 3-4 stages of the cardiovascular system and \ or benign and malignant neoplasms 1-2, 2- 3, 3-4 respiratory phases and \ or benign and malignant neoplasms 1 -2.
2- 3, the steps 3-4 of the gastrointestinal tract and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3 and 3-4 stages of liver and gallbladder in the presence of strongly contaminated samples and biomaterial
- cancer stage 4 of the central nervous system and \ or cancer stage 4 Endocrine and \ or malignancy 4 stages of the reproductive system and \ or cancer stage 4 of the cardiovascular system, cancer stage 4 of the gastrointestinal tract and \ or malignant 4 liver tumors, and gallbladder step in the presence of a sterile sample.
10. The method according to claim 1 characterized in that the characteristics of cell clusters set by the presence of:
- benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the central nervous system and \ or benign neoplasms at hypo and \ or functions and the hyper malignancy 1 -2. 2-3 and steps 3-4 of the endocrine system and \ or benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the reproductive system and \ or benign and malignant neoplasms 1-2, 2-3, 3-4 steps kidney and urinary system and \ or benign and malignant neoplasms 1-2, 2-3 and 3-4 stages of the cardiovascular system and \ or benign and malignant neoplasms 1 -2, 2-3, 3-4 respiratory phases and \ and benign or malignant neoplasms of 1-2, 2-3, 3-4 gastrointestinal m stages Act and \ or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3 and 3-4 stages of liver and gallbladder in the presence of congestion in the group, and
- malignancy stages 2-3 and 3-4 of the central nervous system and \ or malignancy 1 -2, 2-3. 3-4 stages of the endocrine system and \ or benign and malignant neoplasms 1 -2, 2-3 and 3-4 stages of the reproductive system and \ or benign and malignant neoplasms 1-2, 2-3, 3-4 kidneys and urinary system steps and \ or malignancy 1-2, 2-3, 3-4 stages of the cardiovascular system and \ or malignancy 1 - 2, 2-3, 3-4 respiratory phases and \ or benign neoplasms and gastroduodenal malignancy 1 -2, 2-3, 3-4 stages of the gastrointestinal tract \ Or benign neoplasms gepatoza liver and \ or benign neoplasms surround (cysts) and malignant neoplasms 1 -2, 2-3 and 3-4 stages of liver and gallbladder in the presence of clusters in tracks and
- malignancy stage 3-4 of the central nervous system and \ or malignancy stages 2-3 and 3-4 of the endocrine system and \ or malignancy stages 2-3 and 3-4 of the reproductive system and \ or malignancy 1-2 2 3, steps 3-4 kidneys and urinary system and \ or malignancy stages 2-3 and 3-4 of the cardiovascular system and \ or malignancy stage 3-4 of the respiratory system and \ or malignancy 2-3 and 3- 4 stages of the gastrointestinal tract and \ and and malignant neoplasm of 1-2, 2-3 and 3-4 stages of liver and gallbladder in the presence of congestion in curls.
PCT/UA2015/000039 2014-12-05 2015-04-29 Method for rapid diagnosis of benign and malignant neoplasms and associated diseases WO2016089336A1 (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA35478U (en) * 2008-03-19 2008-09-25 Михайло Олександрович Количев Method for diagnosing
UA61495U (en) * 2010-12-06 2011-07-25 Харьковский Национальный Университет Радиоэлектроники A method for diagnosis of malignant and benign tumors
UA92715U (en) * 2014-04-15 2014-08-26 "Лидомед Био Инкорпорейтед" Ооо Method for diagnosing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA35478U (en) * 2008-03-19 2008-09-25 Михайло Олександрович Количев Method for diagnosing
UA61495U (en) * 2010-12-06 2011-07-25 Харьковский Национальный Университет Радиоэлектроники A method for diagnosis of malignant and benign tumors
UA92715U (en) * 2014-04-15 2014-08-26 "Лидомед Био Инкорпорейтед" Ооо Method for diagnosing

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