WO2016081060A3 - Systems for detecting target biological molecules - Google Patents

Systems for detecting target biological molecules Download PDF

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Publication number
WO2016081060A3
WO2016081060A3 PCT/US2015/051095 US2015051095W WO2016081060A3 WO 2016081060 A3 WO2016081060 A3 WO 2016081060A3 US 2015051095 W US2015051095 W US 2015051095W WO 2016081060 A3 WO2016081060 A3 WO 2016081060A3
Authority
WO
WIPO (PCT)
Prior art keywords
complex
target biological
channel
systems
corresponding method
Prior art date
Application number
PCT/US2015/051095
Other languages
French (fr)
Other versions
WO2016081060A2 (en
Inventor
Wayne A. Weimer
Monika J. WILSON
Justin L. ABELL
Shuyun Wu
Original Assignee
Agiltron, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agiltron, Inc. filed Critical Agiltron, Inc.
Publication of WO2016081060A2 publication Critical patent/WO2016081060A2/en
Publication of WO2016081060A3 publication Critical patent/WO2016081060A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/088—Channel loops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces

Abstract

A system, and corresponding method, for detecting a target biological molecule can include a reaction chamber enabling formation of a bead complex, a flowpath that transports the complex to an analysis region, and a spectrometer configured to analyze the complex. A dielectrophoretic pump, and a corresponding method, includes a channel layer with a microfluidic channel defined therein, an electrode layer adjacent to the channel layer including sets of mutually interdigitated electrodes, and a controller to drive the electrodes to cause directional travel of inorganic beads in the channel. Embodiments enable rapid, simultaneous analysis of multiple biological agents on a mobile platform.
PCT/US2015/051095 2014-11-18 2015-09-18 Systems for detecting target biological molecules WO2016081060A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201462081563P 2014-11-18 2014-11-18
US201462081558P 2014-11-18 2014-11-18
US62/081,558 2014-11-18
US62/081,563 2014-11-18
US201562207800P 2015-08-20 2015-08-20
US62/207,800 2015-08-20

Publications (2)

Publication Number Publication Date
WO2016081060A2 WO2016081060A2 (en) 2016-05-26
WO2016081060A3 true WO2016081060A3 (en) 2016-11-10

Family

ID=55315692

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/051095 WO2016081060A2 (en) 2014-11-18 2015-09-18 Systems for detecting target biological molecules

Country Status (1)

Country Link
WO (1) WO2016081060A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019144966A1 (en) * 2018-01-29 2019-08-01 Coyote Bioscience Co., Ltd. Systems and methods for analyzing nucleic acids
CN108709880B (en) * 2018-05-28 2020-10-16 中国工程物理研究院化工材料研究所 Reusable high-flux SERS micro-fluidic chip and application thereof
US20220003773A1 (en) * 2018-11-15 2022-01-06 Ohio State Innovation Foundation Dna-cage erasable labels for fluorescence-based pathology

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070048746A1 (en) * 2005-09-01 2007-03-01 Intel Corporation Multiplex data collection and analysis in bioanalyte detection
WO2008050109A1 (en) * 2006-10-23 2008-05-02 University Of Strathclyde Functionalised polymers for binding metal surfaces
KR20120017358A (en) * 2010-08-18 2012-02-28 한양대학교 산학협력단 Surface-enhanced raman scattering based microfluidic chip for immunoassay and on-chip immunoassay using the same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5082830A (en) 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070048746A1 (en) * 2005-09-01 2007-03-01 Intel Corporation Multiplex data collection and analysis in bioanalyte detection
WO2008050109A1 (en) * 2006-10-23 2008-05-02 University Of Strathclyde Functionalised polymers for binding metal surfaces
KR20120017358A (en) * 2010-08-18 2012-02-28 한양대학교 산학협력단 Surface-enhanced raman scattering based microfluidic chip for immunoassay and on-chip immunoassay using the same

Also Published As

Publication number Publication date
WO2016081060A2 (en) 2016-05-26

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