WO2016071683A3 - Segmented rna genome viruses and their manipulation - Google Patents

Segmented rna genome viruses and their manipulation Download PDF

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Publication number
WO2016071683A3
WO2016071683A3 PCT/GB2015/053303 GB2015053303W WO2016071683A3 WO 2016071683 A3 WO2016071683 A3 WO 2016071683A3 GB 2015053303 W GB2015053303 W GB 2015053303W WO 2016071683 A3 WO2016071683 A3 WO 2016071683A3
Authority
WO
WIPO (PCT)
Prior art keywords
complementarity
rna genome
manipulation
segment
segmented rna
Prior art date
Application number
PCT/GB2015/053303
Other languages
French (fr)
Other versions
WO2016071683A2 (en
Inventor
Mark Boyce
Malcolm MCCRAE
Gillian PULLINGER
Original Assignee
The Pirbright Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Pirbright Institute filed Critical The Pirbright Institute
Publication of WO2016071683A2 publication Critical patent/WO2016071683A2/en
Publication of WO2016071683A3 publication Critical patent/WO2016071683A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12111—Orbivirus, e.g. bluetongue virus
    • C12N2720/12134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12111—Orbivirus, e.g. bluetongue virus
    • C12N2720/12161—Methods of inactivation or attenuation
    • C12N2720/12162—Methods of inactivation or attenuation by genetic engineering

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The present invention provides a segmented virus which comprises a RNA genome comprising a first and a second segment which interact during virion assembly by base pairing between complementarity motifs on the first and second segments; wherein the complementarity motifs on the first and second segments are mutated such that base pairing is maintained between the first and second segment but such that the complementarity motif does not base pair with an equivalent interacting complementarity motif on a segment from a wild type virus.
PCT/GB2015/053303 2014-11-03 2015-11-03 Virus WO2016071683A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1419572.1 2014-11-03
GBGB1419572.1A GB201419572D0 (en) 2014-11-03 2014-11-03 Virus

Publications (2)

Publication Number Publication Date
WO2016071683A2 WO2016071683A2 (en) 2016-05-12
WO2016071683A3 true WO2016071683A3 (en) 2016-06-23

Family

ID=52118635

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2015/053303 WO2016071683A2 (en) 2014-11-03 2015-11-03 Virus

Country Status (2)

Country Link
GB (1) GB201419572D0 (en)
WO (1) WO2016071683A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2811093T3 (en) 2014-11-13 2021-03-10 Univ Geneve Tri-segmented arenaviruses as vaccine vectors
US10669315B2 (en) 2015-06-10 2020-06-02 Hookipa Biotech Gmbh HPV vaccines
EP4177348A1 (en) 2015-11-04 2023-05-10 Hookipa Biotech GmbH Vaccines against hepatitis b virus
HRP20221167T1 (en) 2015-11-12 2022-12-09 Hookipa Biotech Gmbh Arenavirus particles as cancer vaccines
CN110643740B (en) * 2019-10-15 2023-08-04 云南省畜牧兽医科学院 Real-time fluorescent quantitative RT-PCR detection primer, probe and detection kit for Pariemam serogroup virus

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CALVO-PINILLA EVA ET AL: "Recombinant vaccines against bluetongue virus", VIRUS RESEARCH, vol. 182, 25 November 2013 (2013-11-25), pages 78 - 86, XP028634088, ISSN: 0168-1702, DOI: 10.1016/J.VIRUSRES.2013.11.013 *
CATCHPOLE A P ET AL: "Alternative base pairs attenuate influenza A virus when introduced into the duplex region of the conserved viral RNA promoter of either the NS or the PA gene.", JOURNAL OF GENERAL VIROLOGY, vol. 84, no. 3, March 2003 (2003-03-01), pages 507 - 515, XP002754308, ISSN: 0022-1317 *
GAVAZZI CYRILLE ET AL: "A functional sequence-specific interaction between influenza A virus genomic RNA segments.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 8 OCT 2013, vol. 110, no. 41, 8 October 2013 (2013-10-08), pages 16604 - 16609, XP002754307, ISSN: 1091-6490 *
RAO C D ET AL: "Homologous terminal sequences of the genome double-stranded RNAs of bluetongue virus.", JOURNAL OF VIROLOGY MAY 1983, vol. 46, no. 2, May 1983 (1983-05-01), pages 378 - 383, XP002754309, ISSN: 0022-538X *

Also Published As

Publication number Publication date
GB201419572D0 (en) 2014-12-17
WO2016071683A2 (en) 2016-05-12

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