WO2016069853A3 - Methods of detecting ebola - Google Patents

Methods of detecting ebola Download PDF

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Publication number
WO2016069853A3
WO2016069853A3 PCT/US2015/057973 US2015057973W WO2016069853A3 WO 2016069853 A3 WO2016069853 A3 WO 2016069853A3 US 2015057973 W US2015057973 W US 2015057973W WO 2016069853 A3 WO2016069853 A3 WO 2016069853A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
detecting
ebola
absence
sample
Prior art date
Application number
PCT/US2015/057973
Other languages
French (fr)
Other versions
WO2016069853A2 (en
Inventor
Reuel Vanatta
Original Assignee
Cepheid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cepheid filed Critical Cepheid
Publication of WO2016069853A2 publication Critical patent/WO2016069853A2/en
Publication of WO2016069853A3 publication Critical patent/WO2016069853A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Compositions and methods for detecting Ebola are provided. The methods relate to detecting the presence or absence of Ebola virus in a sample from a subject comprising detecting the presence or absence of at least one Ebola gene selected from glycoprotein (GP) and nucleocapsid protein (NP) in the sample, in particular by quantitative PCR. The compositions comprise primers and probes for said quantitative PCR.
PCT/US2015/057973 2014-10-30 2015-10-29 Methods of detecting ebola WO2016069853A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462072989P 2014-10-30 2014-10-30
US62/072,989 2014-10-30

Publications (2)

Publication Number Publication Date
WO2016069853A2 WO2016069853A2 (en) 2016-05-06
WO2016069853A3 true WO2016069853A3 (en) 2016-06-23

Family

ID=54477371

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/057973 WO2016069853A2 (en) 2014-10-30 2015-10-29 Methods of detecting ebola

Country Status (1)

Country Link
WO (1) WO2016069853A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000000617A2 (en) * 1998-06-29 2000-01-06 U.S. Medical Research Institute Of Infectious Diseases Ebola virion proteins expressed from venezuelan equine encephalitis (vee) virus replicons
CN102286641A (en) * 2011-08-18 2011-12-21 深圳国际旅行卫生保健中心 Bioterrorism factor detection chip, detection kit and detection method
US20120178091A1 (en) * 2011-01-06 2012-07-12 Meso Scale Technologies, Llc Assay Cartridges and Methods of Using the Same
CN103045754A (en) * 2011-10-14 2013-04-17 中国农业科学院上海兽医研究所 One-step process real-time fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method and kit for detecting Z/S subtype ebola viruses
CN103045755A (en) * 2011-10-14 2013-04-17 中国农业科学院上海兽医研究所 Fluorescent quantitative PCR (polymerase chain reaction) method, primer and kit for detecting EBOV (Ebola virus)

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
CA2244891C (en) 1996-02-09 2008-12-30 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US5958349A (en) 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US6027998A (en) 1997-12-17 2000-02-22 Advanced Micro Devices, Inc. Method for fully planarized conductive line for a stack gate
JP4209589B2 (en) 1997-12-24 2009-01-14 シーフィード Integrated fluid handling cartridge
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6818185B1 (en) 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
ATE278771T1 (en) 1999-05-28 2004-10-15 Cepheid APPARATUS AND METHOD FOR ANALYZING LIQUID SAMPLES
US6403037B1 (en) 2000-02-04 2002-06-11 Cepheid Reaction vessel and temperature control system
EP1313880A2 (en) 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7351538B2 (en) 2004-08-23 2008-04-01 U.S. Genomics Systems and methods for detecting and analyzing polymers

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000000617A2 (en) * 1998-06-29 2000-01-06 U.S. Medical Research Institute Of Infectious Diseases Ebola virion proteins expressed from venezuelan equine encephalitis (vee) virus replicons
US20120178091A1 (en) * 2011-01-06 2012-07-12 Meso Scale Technologies, Llc Assay Cartridges and Methods of Using the Same
CN102286641A (en) * 2011-08-18 2011-12-21 深圳国际旅行卫生保健中心 Bioterrorism factor detection chip, detection kit and detection method
CN103045754A (en) * 2011-10-14 2013-04-17 中国农业科学院上海兽医研究所 One-step process real-time fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method and kit for detecting Z/S subtype ebola viruses
CN103045755A (en) * 2011-10-14 2013-04-17 中国农业科学院上海兽医研究所 Fluorescent quantitative PCR (polymerase chain reaction) method, primer and kit for detecting EBOV (Ebola virus)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Xpert Ebola Assay Instructions for Use For Use Under an Emergency Use Authorization (EUA) Only", 1 March 2015 (2015-03-01), pages 1 - 21, XP055235639, Retrieved from the Internet <URL:http://www.fda.gov/downloads/MedicalDevices/Safety/EmergencySituations/UCM439578.pdf> [retrieved on 20151211] *
BENJAMIN A. PINSKY ET AL: "Analytical Performance Characteristics of the Cepheid GeneXpert Ebola Assay for the Detection of Ebola Virus", PLOS ONE, vol. 10, no. 11, 12 November 2015 (2015-11-12), pages e0142216, XP055235198, DOI: 10.1371/journal.pone.0142216 *
CRISTIAN APETREI ET AL: "Sequence analysis of the GP, NP, VP40 and VP24 genes of Ebola virus isolated from deceased, surviving and asymptomatically infected individuals during the 1996 outbreak in Gabon: comparative studies and phylogenetic characterization", JOURNAL OF GENERAL VIROLOGY., vol. 83, no. 1, 1 January 2002 (2002-01-01), GB, pages 67 - 73, XP055235635, ISSN: 0022-1317, DOI: 10.1099/0022-1317-83-1-67 *
LEROY E ET AL: "Human asymptomatic Ebola infection and strong inflammatory response", THE LANCET, THE LANCET PUBLISHING GROUP, GB, vol. 355, no. 9222, 24 June 2000 (2000-06-24), pages 2210 - 2215, XP004822527, ISSN: 0140-6736, DOI: 10.1016/S0140-6736(00)02405-3 *

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Publication number Publication date
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