WO2016058338A1 - 一种免疫印迹检测板反应装置 - Google Patents

一种免疫印迹检测板反应装置 Download PDF

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Publication number
WO2016058338A1
WO2016058338A1 PCT/CN2015/075666 CN2015075666W WO2016058338A1 WO 2016058338 A1 WO2016058338 A1 WO 2016058338A1 CN 2015075666 W CN2015075666 W CN 2015075666W WO 2016058338 A1 WO2016058338 A1 WO 2016058338A1
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tray
groove
hole
circular
shaped
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PCT/CN2015/075666
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English (en)
French (fr)
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吴善东
沈华浩
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杭州浙大迪迅生物基因工程有限公司
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Application filed by 杭州浙大迪迅生物基因工程有限公司 filed Critical 杭州浙大迪迅生物基因工程有限公司
Priority to US15/303,891 priority Critical patent/US20170225170A1/en
Priority to DE212015000183.0U priority patent/DE212015000183U1/de
Publication of WO2016058338A1 publication Critical patent/WO2016058338A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/50—Clamping means, tongs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis

Definitions

  • the utility model relates to an immunoblot detection plate reaction device.
  • the detection trend of immunoblotting products is to adopt a fully automatic operation instrument, which is safe and easy to operate.
  • Such instruments are costly and are not suitable for use by general primary medical institutions.
  • the imprinting automatic operation instrument produced by the German EU company and the automatic Western blotting instrument produced by the Austrian TECAN company are equipped with a blotting detection strip reaction tray, but this type of tray is only suitable for detecting the imprinted bare strip, for the detection of the reaction groove
  • the board is not suitable; and the process of sticking the bare strips is time consuming and laborious, and there is a risk of biohazard in the process of the operator touching the strips by hand.
  • the immunoblot detection plate reaction scaffold invented by the German company Mediwiss-analytic can fix the test strip well, and realize the steps of washing and replacing the reagent during the manual operation.
  • the reaction scaffold can simultaneously detect multi-person samples and is suitable for a wide range.
  • the reaction bracket needs to be installed and removed by the insertion and removal operation. Because the force receiving area is small, the insertion and removal process is relatively laborious, and the finger easily touches the surface of the membrane reagent in the reaction tank during the insertion and removal process, resulting in Background pollution, it is easy to cause the card slot to break after being inserted and removed many times.
  • the utility model provides a convenient installation and removal of the detection plate, and avoids the laborious insertion and removal of the detection plate.
  • An immunoblot detection plate reaction device which can satisfy both manual operation and instrument operation, has high practicability and wide application range.
  • the invention relates to an immunoblot detection plate reaction device according to the present invention, which is characterized in that it comprises a tray holder and at least one tray, and the upper surface of the tray is provided with a plurality of grooves for placing the detection plates, and each The bottom surface of the groove is provided with a bayonet for engaging the corresponding detecting plate, and the tray groove is completely fitted with the detecting plate, and one side of the tray is provided with a snap button for fastening on the tray frame, and the bottom surface of the tray is provided There is a tray backrest for placing on the automatic immunoblotting device, the snap button comprises a fastening portion and a pressing portion, and one end of the fastening portion is fixed to the edge of the tray, and the other end and the pressing portion are fixed.
  • the tray holder includes a tray slot and a snap tab, the snap tab is disposed at an edge of the tray slot, and the pressing tab is engaged with the tray Department fastening.
  • All the grooves are evenly arranged on the upper surface of the tray in the same direction, and each groove corresponds to a numbered groove.
  • the numbered groove is solid or hollow, and the groove shape of the numbered groove is rectangular, square, semi-circular, elliptical, fan-shaped, trapezoidal, rhombic, triangular or other suitable shape, and the numbered form is Arabic numerals and Chinese lowercase. Numbers, Roman numerals, English letters or other suitable forms.
  • the groove is solid or hollow, and the groove body of the groove is open at least one square hole, rectangular hole, elliptical hole, circular hole, diamond hole, triangular hole, trapezoidal hole or other suitable shape at any position.
  • the number of holes, the number of openings is 1, 2, 3 or other suitable quantities.
  • the shape of the bayonet is rectangular, square, semi-circular, elliptical, fan-shaped, trapezoidal, and diamond. Shape, triangle or other suitable shape.
  • the fastening portion is a U-shaped, V-shaped or other suitable groove type structure
  • the pressing portion is rectangular, square, circular, semi-circular, elliptical, fan-shaped, trapezoidal, rhombic, triangular or Other suitable shapes, and the snap-fit bayonet shape matches the pressing portion.
  • the bottom surface of the tray is provided with at least one rear surface of the tray for supporting, and the shape of the back surface of the tray is circular, rectangular, square, semi-circular, elliptical, fan-shaped, trapezoidal, rhombic, triangular or other suitable shape.
  • the tray slot of the tray rack is solid or hollow. At least one square hole, a rectangular hole, an elliptical hole, a circular hole, a diamond hole, a triangular hole, a trapezoidal hole or at any position of the tray groove of the tray frame Other suitable shaped holes.
  • the tray and the tray frame are made of ABS, PC, PA, POM, PPE, PETP, PBTP, PPS or other suitable engineering plastics, including adding 20-30% proportion or other suitable proportion of the glass in the above materials. Fiber material.
  • the utility model has the beneficial effects that the tray can be easily installed and removed, and the tray groove is completely fitted with the detecting plate, thereby ensuring that the detecting plate is in a stable state during the washing and incubation process.
  • the reaction tray can be used not only for manual operation but also for instrument operation.
  • multiple trays can be placed on the tray rack at the same time, and the specimens of multiple persons can be scanned at one time, which greatly facilitates the processing of the detection results.
  • Figure 1 is a front elevational view of the present invention.
  • Fig. 2 is a cross-sectional view taken along line A-A of Fig. 1;
  • Figure 3 is an exploded view of Figure 1.
  • Fig. 4 is a cross-sectional view taken along line B-B of Fig. 1;
  • Fig. 5 is a schematic view showing the position of the tray, the tray holder and the detecting board of the present invention.
  • Figure 6 is a second schematic view of the position of the tray, the tray holder and the detecting board of the present invention.
  • Figure 7 is a front elevational view of the tray and tray holder of the present invention.
  • Fig. 8 is a cross-sectional view taken along line A-A of Fig. 1;
  • Fig. 9 is a cross-sectional view taken along line B-B of Fig. 1;
  • Figure 10 is a front elevational view of the tray holder of the present invention.
  • Figure 11 is a bottom plan view of the tray carrier of the present invention.
  • Figure 12 is a front elevational view of the tray holder of the present invention.
  • Figure 13 is a bottom plan view of the tray carrier of the present invention.
  • Figure 14 is a structural view of the detecting plate of the present invention.
  • Figure 15 is a second structural view of the detecting plate of the present invention.
  • An immunoblot detection plate reaction device comprises a tray rack 1, at least one tray 2, and the upper surface of the tray 2 is provided with a plurality of grooves 21 for placing the detecting plates. And the bottom surface of each groove 21 is provided with a bayonet 22 for clamping the corresponding detecting plate, and the groove 21 of the tray 2 is completely fitted with the detecting plate 3, said One side of the tray 2 is provided with a snap button 23 for fastening to the tray frame 1.
  • the bottom surface of the tray 2 is provided with a tray back surface 24 for placing on the fully automatic immunoblotting instrument, and the snap button 23 includes a snap fit.
  • the portion of the fastening portion 231 is fixed to the edge of the tray 2 and the other end is fixed to the pressing portion 232.
  • the tray holder 1 includes a tray slot 11 and a snap tab 12 The snap-in tab 12 is disposed at an edge of the tray slot 11 and the pressing tab 12 is engaged with the latching portion 241 of the tray 2.
  • All the grooves 21 are evenly arranged on the upper surface of the tray 2 in the same direction, and each of the grooves 21 corresponds to one numbering groove 25.
  • the numbering groove 25 is solid or hollow, and the groove shape of the numbering groove is rectangular, square, semi-circular, elliptical, fan-shaped, trapezoidal, diamond, triangular or other suitable shape, and the numbered form is Arabic numerals, Chinese. Lowercase numbers, Roman numerals, English letters, or other suitable form.
  • the groove 21 is solid or hollow, and the groove body of the groove 21 is at least one square hole, rectangular hole, elliptical hole, circular hole, diamond hole, triangular hole, trapezoidal hole or other suitable position at any position.
  • the shape of the hole, the number of openings is 1, 2, 3 or other suitable number.
  • the bayonet 22 is shaped as a rectangle, a square, a semicircle, an ellipse, a sector, a trapezoid, a diamond, a triangle, or other suitable shape.
  • the fastening portion 241 is a U-shaped, V-shaped or other suitable recessed structure, and the pressing portion is rectangular, square, circular, semi-circular, elliptical, fan-shaped, trapezoidal, rhombic, triangular. Or other suitable shape, and the snap-fit bayonet 12 is shaped and The pressing portions match.
  • At least one bottom surface of the tray 2 is provided at any position on the bottom surface of the tray 2.
  • the back surface of the tray 24 is circular, rectangular, square, semi-circular, elliptical, fan-shaped, trapezoidal, rhombic, triangular. Or other suitable shape.
  • the tray body 11 of the tray rack 1 is solid or hollow, and at least one square hole, a rectangular hole, an elliptical hole, a circular hole, a diamond hole, a triangle is opened at any position of the tray groove 11 of the tray. Holes, trapezoidal holes or other suitable shaped holes.
  • the tray 2 and the tray rack 1 are made of ABS, PC, PA, POM, PPE, PETP, PBTP, PPS or other suitable engineering plastics, including adding 20-30% ratio or other suitable ratio to the plastic of the above materials.
  • Example 2 Using the apparatus provided in Example 1, immunoblotting was performed, and the specimen information was marked on the handle of the test plate in the inhalation-food allergen-specific antibody IgE detection kit 2 (immunoblotting method). Hold the end of the test board handle, align the check board of the reaction tray, and gently load the test board into the tray. Each tray can hold 1-100 test panels at the same time.
  • the reaction tray After drying all the test plate membrane surfaces and the reaction tank on the reaction tray with a hair dryer, the reaction tray is placed on the tray holder, and then the tray holder is reversed on the scanner, and the test result is interpreted by the supporting software.

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  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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Abstract

一种免疫印迹检测板反应装置，包括一个托盘架（1）、至少一个托盘（2），托盘（2）的上表面设有若干个用于放置检测板（3）的凹槽（21），每个凹槽（21）底面配有用于卡住相应检测板（3）的卡口（22），凹槽（21）与检测板（3）完全贴合，托盘（2）的一侧设有用于扣合在托盘架（1）上的按扣（23），托盘（2）底面设有用于放置在全自动免疫印迹仪上的托盘背面卡口（24），按扣（23）包括扣合部（231）和按压部（232），扣合部（231）一端与托盘（2）边缘固定、另一端与按压部（232）固接；托盘架（1）包括托盘槽体（11）和按扣卡口（12），按扣卡口（12）设置在托盘槽体（11）的边缘，按压卡口（12）与扣合部（231）扣合，使得托盘（2）和检测板（3）能够方便的安装和取下，检测板（3）无论在洗板还是孵育过程中均处于平稳的状态；还能够一次性扫描多人份标本，方便检测结果的处理。

Description

一种免疫印迹检测板反应装置 技术领域
本实用新型涉及一种免疫印迹检测板反应装置。
背景技术
目前，免疫印迹类产品的检测趋势是采用全自动操作仪，该类仪器安全性强、操作简便。但该类仪器成本较高，不适合一般基层医疗机构使用。德国欧盟公司生产的印迹法自动操作仪和奥地利TECAN公司生产的全自动蛋白印迹仪均配有印迹检测膜条反应托盘，但该类托盘只适用于检测印迹裸条，对于带反应凹槽的检测板并不适用；且粘贴印迹裸条的过程比较费时费力，操作者用手接触试剂条的过程中还存在生物危害风险。德国Mediwiss-analytic公司发明的免疫印迹检测板反应支架，可以很好的固定检测板条，实现手工操作过程中试剂的洗涤、更换液体等步骤。该反应支架可同时实现多人份样本的检测，适合范围广。但该反应支架需通过借助插拔动作实现检测板的安装和取下，由于受力面积小，插拔过程比较费力，而且插拔过程中手指容易碰触到反应槽中膜试剂的表面，造成背景污染，插拔多次后还容易造成卡槽的断裂。
发明内容
为了解决目前的全自动操作仪成本高以及现有的反应支架插拔费力、而且插拔过程中手指容易碰触到反应槽中膜试剂的表面，造成背景污染，插拔多次后还容易造成卡槽的断裂的问题，本实用新型提出了一种可以方便检测板的安装和取下，避免了检测板费力的插拔动 作、并可同时满足手工操作和仪器操作，实用性强，适用范围广的一种免疫印迹检测板反应装置。
本实用新型所述的一种免疫印迹检测板反应装置，其特征在于：包括一个托盘架、至少一个托盘，所述的托盘的上表面设有若干个用于放置检测板的凹槽，并且每个凹槽底面配有用于卡住相应检测板的卡口，并且托盘凹槽与检测板完全贴合，所述的托盘的一侧设有用于扣合在托盘架上的按扣、托盘底面设有用于放置在全自动免疫印迹仪上的托盘背面卡口，所述的按扣包括扣合部和按压部，所述的扣合部一端与所述的托盘边缘固定、另一端与按压部固接；所述的托盘架包括托盘槽体和按扣卡口，所述的按扣卡口设置在所述的托盘槽体的边缘，并且所述的按压卡口与所述的托盘的扣合部扣合。
所有的凹槽沿同一方向均匀排列在托盘上表面，并且每个凹槽对应一个编号槽。
所述的编号槽为实心或空心，所述的编号槽的槽形状为长方形、正方形、半圆形、椭圆形、扇形、梯形、菱形、三角形或其他适宜形状，编号形式为阿拉伯数字、中文小写数字、罗马数字、英文字母或其他适宜的形式。
所述的凹槽为实心或空心，所述的凹槽的槽体任意位置开至少一个正方形孔、长方形孔、椭圆形孔、圆形孔、菱形孔、三角形孔、梯形孔或其他适宜形状的孔，开孔数量为1个、2个、3个或其他适宜数量。
卡口形状为长方形、正方形、半圆形、椭圆形、扇形、梯形、菱 形、三角形或其他适宜形状。
所述的扣合部为U型、V型或其他适宜性状的凹槽式结构，所述的按压部为长方形、正方形、圆形、半圆形、椭圆形、扇形、梯形、菱形、三角形或其他适宜形状，并且所述的按扣卡口形状与按压部匹配。
所述的托盘底面任意位置设有至少一个用于支撑的托盘背面卡口，托盘背面卡口形状为圆形、长方形、正方形、半圆形、椭圆形、扇形、梯形、菱形、三角形或其他适宜形状。
所述的托盘架的托盘槽体为实心或空心所述托盘架的托盘槽体的任意位置开至少一个正方形孔、长方形孔、椭圆形孔、圆形孔、菱形孔、三角形孔、梯形孔或其他适宜形状的孔。
所述的托盘和托盘架材质为ABS、PC、PA、POM、PPE、PETP、PBTP、PPS或其他适宜的工程塑料，包括在上述材质的塑料中添加20-30％比例或其他适宜比例的玻璃纤维材质。
本实用新型的有益效果是：托盘可以轻松的安装和取下检测板，托盘凹槽与检测板完全贴合，保证了检测板无论在洗板还是孵育过程中均处于平稳的状态。同时，该反应托盘不但可以用于手工操作而且可用于仪器操作。另外，实验完成后，可同时将多个托盘置于托盘架上，一次性扫描多人份的标本，极大的方便了检测结果的处理。
附图说明
图1是本实用新型的主视图。
图2是图1的A-A剖视图。
图3是图1的爆炸图。
图4是图1的B-B剖视图。
图5是本实用新型的托盘、托盘架以及检测板的位置示意图之一。
图6是本实用新型的托盘、托盘架以及检测板的位置示意图之二。
图7是本实用新型的托盘和托盘架安装正视图。
图8是图1的A-A剖视图。
图9是图1的B-B剖视图。
图10是本实用新型的托盘架正视图。
图11是本实用新型的托盘架仰视图。
图12是本实用新型的托盘架正视图。
图13是本实用新型的托盘架仰视图。
图14是本实用新型的检测板的结构图之一。
图15是本实用新型的检测板的结构图之二。
具体实施方式
下面结合附图进一步说明本实用新型
参照附图：
实施例1本实用新型所述的一种免疫印迹检测板反应装置，包括一个托盘架1、至少一个托盘2，所述的托盘2的上表面设有若干个用于放置检测板的凹槽21，并且每个凹槽21底面配有用于卡住相应检测板的卡口22，并且托盘2凹槽21与检测板3完全贴合，所述的 托盘2的一侧设有用于扣合在托盘架1上的按扣23、托盘2底面设有用于放置在全自动免疫印迹仪上的托盘背面卡口24，所述的按扣23包括扣合部231和按压部232，所述的扣合部231一端与所述的托盘2边缘固定、另一端与按压部232固接；所述的托盘架1包括托盘槽体11和按扣卡口12，所述的按扣卡口12设置在所述的托盘槽体11的边缘，并且所述的按压卡口12与所述的托盘2的扣合部241扣合。
所有的凹槽21沿同一方向均匀排列在托盘2上表面，并且每个凹槽21对应一个编号槽25。
所述的编号槽25为实心或空心，所述的编号槽的槽形状为长方形、正方形、半圆形、椭圆形、扇形、梯形、菱形、三角形或其他适宜形状，编号形式为阿拉伯数字、中文小写数字、罗马数字、英文字母或其他适宜的形式。
所述的凹槽21为实心或空心，所述的凹槽21的槽体任意位置开至少一个正方形孔、长方形孔、椭圆形孔、圆形孔、菱形孔、三角形孔、梯形孔或其他适宜形状的孔，开孔数量为1个、2个、3个或其他适宜数量。
卡口22形状为长方形、正方形、半圆形、椭圆形、扇形、梯形、菱形、三角形或其他适宜形状。
所述的扣合部241为U型、V型或其他适宜性状的凹槽式结构，所述的按压部为长方形、正方形、圆形、半圆形、椭圆形、扇形、梯形、菱形、三角形或其他适宜形状，并且所述的按扣卡口12形状与 按压部匹配。
所述的托盘2底面任意位置设有至少一个用于支撑的托盘背面卡口24，托盘背面卡口24形状为圆形、长方形、正方形、半圆形、椭圆形、扇形、梯形、菱形、三角形或其他适宜形状。
所述的托盘架1的托盘槽体11为实心或空心，所述托盘,1的托盘槽体11的任意位置开至少一个正方形孔、长方形孔、椭圆形孔、圆形孔、菱形孔、三角形孔、梯形孔或其他适宜形状的孔。
所述的托盘2和托盘架1材质为ABS、PC、PA、POM、PPE、PETP、PBTP、PPS或其他适宜的工程塑料，包括在上述材质的塑料中添加20-30％比例或其他适宜比例的玻璃纤维材质。
实施例2利用实施例1提供的装置，进行免疫印迹法测定，在吸入性-食物性过敏原特异性抗体IgE检测试剂盒2(免疫印迹法)中的检测板手柄处标注上标本信息，用手握住检测板末端手柄，对准反应托盘的检测板卡口，轻轻将检测板装入托盘内。每个托盘可同时放置1-100个检测板。
在已安装于托盘上的检测板内加入洗涤液，使膜条充分润湿3分钟，润湿后，一只手拿住反应托盘编号槽位置，将检测板内的洗涤液倒掉后甩干。
在各检测板内加入300微升相应的血清标本，将托盘置于脱色摇床上，室温(20-25℃)孵育45分钟后，一手拿起反应托盘，将检测板内的液体顺着槽体小心倾倒掉，然后用洗瓶中的洗涤液来回冲洗检 测板膜面5次后甩干。
在各检测板内加入300微升二抗工作液，将托盘置于脱色摇床上，室温(20-25℃)孵育45分钟后，一只手拿住托盘编号槽位置，将检测板内的液体顺着槽体小心倾倒掉，然后用洗瓶中的洗涤液来回冲洗检测板膜面5次后甩干。
在各检测板内加入300微升酶工作液，将托盘置于脱色摇床上，室温(20-25℃)孵育45分钟后，一只手拿住托盘编号槽位置，将检测板内的液体顺着槽体小心倾倒掉，然后用洗瓶中的洗涤液来回冲洗检测板膜面5次后甩干。
在各检测板内加入300微升底物溶液，将托盘置于脱色摇床上，室温(20-25℃)孵育45分钟后，一手拿起托盘，将检测板内的液体顺着槽体小心倾倒掉，并用自来水将检测板彻底冲洗干净。
用电吹风将反应托盘上所有检测板膜面和反应槽吹干后，将反应托盘置于托盘架上，然后将托盘架倒扣在扫描仪上，通过配套软件判读检测结果。
本说明书实施例所述的内容仅仅是对实用新型构思的实现形式的列举，本实用新型的保护范围不应当被视为仅限于实施例所陈述的具体形式，本实用新型的保护范围也包括本领域技术人员根据本实用新型构思所能够想到的等同技术手段。

Claims (8)

  1. 一种免疫印迹检测板反应装置，其特征在于：包括一个托盘架、至少一个托盘，所述的托盘的上表面设有若干个用于放置检测板的凹槽，并且每个凹槽底面配有用于卡住相应检测板的卡口，并且托盘凹槽与检测板完全贴合，所述的托盘的一侧设有用于扣合在托盘架上的按扣、托盘底面设有用于放置在全自动免疫印迹仪上的托盘背面卡口，所述的按扣包括扣合部和按压部，所述的扣合部一端与所述的托盘边缘固定、另一端与按压部固接；所述的托盘架包括托盘槽体和按扣卡口，所述的按扣卡口设置在所述的托盘槽体的边缘，并且所述的按压卡口与所述的托盘的扣合部扣合。
  2. 如权利要求1所述的一种免疫印迹检测板反应装置，其特征在于：所有的凹槽沿同一方向均匀排列在托盘上表面，并且每个凹槽对应一个编号槽。
  3. 如权利要求2所述的一种免疫印迹检测板反应装置，其特征在于：编号槽槽形状为长方形、正方形、半圆形、椭圆形、扇形、梯形、菱形或三角形，编号形式为突起或凹进去的阿拉伯数字、中文小写数字、罗马数字或英文字母。
  4. 如权利要求2所述的一种免疫印迹检测板反应装置，其特征在于：所述的凹槽的槽体任意位置开至少一个正方形孔、长方形孔、椭圆形孔、圆形孔、菱形孔、三角形孔或梯形孔。
  5. 如权利要求1所述的一种免疫印迹检测板反应装置，其特征在于：卡口形状为长方形、正方形、半圆形、椭圆形、扇形、梯形、菱形或三角形。
  6. 如权利要求1所述的一种免疫印迹检测板反应装置，其特征在于：所述的扣合部为U型、V型的凹槽式结构，所述的按压部为长方形、正方形、圆形、半圆形、椭圆形、扇形、梯形、菱形或三角形，并且所述的按扣卡口形状与按压部匹配。
  7. 如权利要求1所述的一种免疫印迹检测板反应装置，其特征在于：所述的托盘底面任意位置设有至少一个用于支撑的托盘背面卡口，托盘背面卡口形状为圆形、长方形、正方形、半圆形、椭圆形、扇形、梯形、菱形或三角形。
  8. 如权利要求1所述的一种免疫印迹检测板反应装置，其特征在于：所述托盘架的托盘槽体的任意位置开至少一个正方形孔、长方形孔、椭圆形孔、圆形孔、菱形孔、三角形孔或梯形孔。
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