WO2016044768A1 - Compositions, procédés, conditionnement et appareil pour améliorer la fertilité des sols et la croissance des plantes - Google Patents

Compositions, procédés, conditionnement et appareil pour améliorer la fertilité des sols et la croissance des plantes Download PDF

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WO2016044768A1
WO2016044768A1 PCT/US2015/051025 US2015051025W WO2016044768A1 WO 2016044768 A1 WO2016044768 A1 WO 2016044768A1 US 2015051025 W US2015051025 W US 2015051025W WO 2016044768 A1 WO2016044768 A1 WO 2016044768A1
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soil
composition
microorganisms
oil
nutrients
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PCT/US2015/051025
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English (en)
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John M. Perraut
Mark LOVIL
Stephen HARKELI
Charles PERRAUT
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Global Soil Solutions, Inc.
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Publication of WO2016044768A1 publication Critical patent/WO2016044768A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C05D9/02—Other inorganic fertilisers containing trace elements
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • Methods and compositions are disclosed that improve soil fertility and plant health. These methods and compositions are useful for treatment of existing crops in fields and the soil in which crops grow, as well as of in-furrow with seeds while planting, transplanting plants and nursery use. Methods also are disclosed to maximize profit of farmers, growers and ranchers resulting from use of the disclosed methods and compositions.
  • Air is present in the pore spaces between soil particles. Because water is the other substance that can occupy significant amounts of pore space, air content is determined to a large extent by how wet soil is. The presence of air— particularly oxygen— in pore spaces is as important to most plants as water. Thus, good aeration is an important physical property of soil. Soils that hold a great deal of water are low or lacking in oxygen. That is why plants languish in saturated soils— their roots starve for oxygen.
  • a composition is disclosed to improve soil fertility in a target soil and enhance yield and health of crops in the soil, transplant environments, seeds and seedlings in a nursery.
  • the composition includes macronutrients, micronutrients, enzymes and microorganisms, wherein the nutrients are in a form that is available to plants, and where enzymes and nutrients are compatible with the microorganisms.
  • Suitable macronutrients include N, K, Ca, Mg, P in the form of, for example, urea, di-potassium phosphate, calcium citrate and magnesium citrate.
  • Suitable micronutrients include Fe, B, Mn, Zn, Cu, and Mo in the form of, for example, ferric ammonium citrate, boric acid, manganese citrate, zinc citrate, molybdic acid. Forms are available for plant absorption and not harmful to the microorganisms.
  • Enzymes e.g. amidase, alpha amylase,
  • exopolysaccharides proteinaceous, poly-y-glutamic acid, subtillisin may also be in the composition.
  • Microorganisms include isolates from the genus Bacillus, Pseudomonas,
  • Rhizobium, Trichoderma, and Azorhizobium for example as listed in U.S. Pat. 8,822, 190 (Cols. 3-8).
  • Single bacterial strains, fungal strains and groups thereof are chosen based on analysis of optimal microorganisms in soils for specific plant growth.
  • the nutrient treatment to address target compositions include one or more
  • ingredients provided in a humic acid and kelp solution e.g. K- Z-...HumiCharge.
  • a pesticide compatible with the microorganisms may be in the composition.
  • Suitable pesticides include Cedar Oil, Guava Oil, or a combination of Cedar oil and Guava oil.
  • the composition is suitably applied to foliage of plants in soil to improve soil fertility in a target soil and/or enhance yield and health of the plants in the soil.
  • Macronutrients, micronutrients and microorganisms are in a form that is available to plants and compatible with the microorganisms.
  • Foliar nutrients including N, P, Ca, Mg, P, S, CI, Fe, B, Mn, Zn, Cu, Mo, and Ni, are in a form available to the plant.
  • Foliar tissue analysis indicates what ingredients are needed in a target soil or on a target crop.
  • microorganisms are determined by the by type and amount of differences between the composition of the target soil and the composition of a desired soil, and the lack of uptake of nutrients that are in the soil, but not found in the tissue analysis.
  • the composition may be in a concentrated liquid or dry form.
  • the enzymes, macronutrients, and micronutrients may start in a dry form.
  • Liquefaction requires a high sheer mixer and/or pump and static mixer. Combining is exothermic and requires active or passive control temperature below 127 °F to prevent reactions forming insoluble compounds at 132 °F. This enables the shipment of the material dry and then mixed on site at the point of use. Equipment can be made small enough to be easily mobile. This results is a tremendous savings in transportation cost.
  • a plant wash that is compatible with, and complimentary to, the microorganisms and the nutrients in the composition is useful.
  • a scrub is effective to help clean and improve function of spray equipment and irrigation systems.
  • the plant wash may include a plant scrub, conditioner, primer and surfactant.
  • the target plants and soil may be treated by:
  • a method to enhance soil fertility in a target soil includes:
  • Targeted treatment can be accomplished in many ways.
  • the nutrient formula may be changed or modified as in Tables 3 and 4.
  • the specific nutrient or nutrients can be added to the blend in the form of a quantity of humic acid with the one or several of the nutrients found missing or deficient added.
  • K- HumiCHARGETM is potasium (K) and humic acid, (and Kelp).
  • C-HumiCHARGE for Calcium
  • M-HumiCHARGE for Magnesium, and so forth.
  • a kit may include containers of scrub, microbes, nutrients, nitrogen, a potassium spike humic acid, test kit, and/or pesticide, descriptions and instructions for use in standard farm spraying equipment or irrigation systems.
  • a method to improve a farmer's "bottom line,” includes reducing costs, improving crop yield and obtaining carbon credits,” by:
  • Soil Fertility may be monetized by taking the percent of Soil Organic Matter (SOM) the equivalent value of the now available nutrient content.%SOM x Value of nutrient.
  • FIG. 1 shows a flow chart of Global Soil Solution Crop Management System
  • FIGs. 2A-B shows a consumer sized six-pack 16 oz makes 2.5 gallons with water where (1) Microbes; (2) Nutrient; (3) Nitrogen; (4) Fulvic acid; (5) K- humic acid; (6) Surfactant; (7) Carrier.
  • FIGs. 3A-B shows a nursery sized 1 gallon makes 20 gallons with water for a lacre application consisting of (1) Surfactant; (2) Microbes; (3) Nutrient; (4) Nitrogen - (in the form of Amino acid powder in a pouch; (5) Fulvic acid; (6) K- humic acid; (7) Test kit -Manila envelope with 2 re-sealable bags and instructions; (8) Shipping box.
  • FIG. 4 shows a soil test result; there was a resulting formula change; in this case the soil test indicated that there is too much copper in the soil from previous applications of copper to stop citrus canker.
  • Compositions are disclosed to improve soil fertility in a target soil and to enhance yield and health of crops (plants) in the soil, transplants and seedlings in a nursery or when seeds are planted.
  • Compositions include macronutrients, micronutrients, enzymes and microorganisms, wherein the nutrients are in a form that is available to plants, and wherein enzymes and nutrients are compatible with the microorganisms.
  • the composition may be in a concentrated liquid or a dry form.
  • Suitable micronutrients include B, Mn, Zn, Cu, and Mo.
  • the micronutrients may be in the form of ferric ammonium citrate, boric acid, manganese citrate, zinc citrate, molybdic acid.
  • exopolysaccharides proteinaceous, poly-y-glutamic acid, subtillisin
  • exopolysaccharides proteinaceous, poly-y-glutamic acid, subtillisin
  • Enzymes are essential to all living organisms.
  • An enzyme is a globular protein that acts as a catalyst, a chemical agent that speeds up reactions without being consumed by the reaction. They lower the activation energy needed for chemical reactions, allowing the reactant molecules to absorb enough energy to reach the transition state. Soil enzymes increase the reaction rate at which plant residues decompose and release plant available nutrients.
  • Soil enzymes play an important role in organic matter decomposition and nutrient cycling Some enzymes only facilitate the breakdown of organic matter ( Alpha Amylase, Exopolysacch-arides, Proteinaceous, Poly-y-glutamic Acid, Subtillisin) while others are involved in nutrient mineralization (e.g., amidase)
  • Enzymes catalytic proteins, speed up chemical reactions. Without enzymes, biological reactions occur at a much slower rate or not at all. Without these biological catalysts, life would not exist.
  • Enzymes work by weakening substrate bonds lowering activation energy (energy necessary for the reaction to occur). The enzyme then releases the product and is ready to begin the process again.
  • activation energy energy necessary for the reaction to occur.
  • One enzyme molecule can act over and over, transforming many substrate molecules.
  • Suitable microorganisms are as listed in U.S. Pat. 8,822, 190. These include representations of the genus Bacillus, Pseudomonas, Rhizobium, Trichodenna, and Azorhizobium.
  • Micronutrients include N, K, Ca, Mg, P, in the form urea, di-potassium phosphate, calcium citrate and magnesium citrate; the micronutrients include Fe, B, Mn, Zn, Cu, Mo, in the form ferric ammonium citrate, boric acid, manganese citrate, zinc citrate, molybdic acid; enzymes include protein enzyme complex (amidase, alpha amylase, exopolysaccharides, proteinaceous, poly-y-glutamic acid, subtillisin).
  • a pesticide compatible with the microorganisms is also a component, e.g., Cedar Oil and/or Guava oil, and or similarly compatible yet effective biopesticide
  • a composition applied to foliage of plants in soil to improve soil fertility in a target soil and enhance yield and health of the plants in the soil includes macronutrients, micronutrients and microorganisms, wherein the nutrients are in a form that is available to plants and compatible with the microorganisms.
  • compositions include "AminoNITETM in amino form and FulviChargeTM fulvic acid.
  • the type and amounts of macronutrients, micronutrients and microorganisms are determined by the type and amount of differences between the composition of the target soil and a composition of a desired soil, and the lack of uptake of nutrients that are in the soil, but are not in the tissue analysis, and recommended pathology recommendations.
  • a plant wash that is compatible with, and complimentary to, the microorganisms and the nutrients is applied to foliage.
  • the plant wash may include a plant scrub, conditioner, primer and surfactant.
  • a suitable plant wash includes ingredients shown in Table 1 :
  • Alkyl Poly Glycoside ( 141464- 16. ) Lemongrass oil 29. ) Processed extracts of sugar 42-8) 17. ) Lilly extract (84696-27-5); cane (57-50-1);
  • Cinnamon oil (8007-80-5) 20.
  • Processed Neem oil 31.
  • Citronella oil (8000-29- 1) 21.
  • Soybean oil (8001-22-7 ; -
  • a kit includes a spray bottle, a paddle and dry components of a composition com ipatible with microbes.
  • the composition may be in dry form.
  • Soil fertility assessment is based on lab testing of:
  • Rhizosphere ecology Plant Growth Promoting Rhizobacteria or PGPR
  • the materials and methods are applicable to, for example, cropland, degraded land and pastures. Improvement in production and quality over time encourages small holders at subsistence level as well as larger commercial farmers, growers and herders to adopt improved management practices and thereby enhance productivity.
  • Plant Growth Promoting Rhizobacteria is useful to maintain the delicate balance of soil ecology. Recent progress in understanding the benefits PGPR facilitates its use a reliable component in the management of a sustainable agricultural. The potential of PGPR in agriculture is steadily increasing as an attractive alternative to chemical fertilizers, pesticides and other chemicals harmful to soil fertility and the environment. (Biosoil Enhancers Inc.)
  • Materials and methods disclosed herein will benefit civilization by a system for improving profits through increasing yields, faster growth, shortens time to market, and higher quality, while reducing costs.
  • Implementation of best practices will reduce pollution by fertilizer and run-off, reduce water usage through soil water retention and deeper roots. Healthy plants with strong immune systems and deep roots require less use of pesticides, and fungicides. Use of biopesticides which do not harm microbes, contribute to soil fertility.
  • Devastation of crops occurs from HLB/ Citrus Greening within the Florida Citrus Industry and there are analogous issues for other crops such as fungus- coffee rust “Roya” , Black leaf spot fungus diseases, "Sigatoka” in bananas and plantains, nematodes and parasites in sugar cane.
  • Depleted soil weakens plants leaving them susceptible and unable to repel insects and fight disease.
  • the guava oil also was reported to "melt any larva or eggs that were laid on the citrus tree foliage and any time of the year, not just the active times.” Guava oil is extremely effective on adults, their eggs or nymphs, psyllid, aphids, mites, scale, ants, roaches, flies, spiders mosquitoes, Weevil, Armyworm, Beetles, Cutworms, Spittlebug, Leafhoppers, Webworm, Thrips, Wireworms Looper, Boll Weevil, Bollworm, Leaf Perforator, Chinch Bug, Grasshoppers, Whiteflies,, Stink Bugs, Hessian Fly, Crane Fly, Bean Bugs, Cutworms, Moth, Flea Beetles, Leafminer, Slugs and the like.
  • a soil analysis is needed to determine the current state of fertility and prescribe the proper application of microbes and nutrients, and to track progress toward the targeted composition and evaluate the financial value.
  • Organic matter resists soil compaction because surface residue resists compaction. It acts like a sponge to absorb weight and water. Organic residues are less dense than soil particles. Roots create voids and spaces for air and water and act like a biological valve to control oxygen in the soil. Roots also supply exudates to glue soil particles together to form macro-aggregates and supply food for microbes. Table 2 illustrates a method for valuing the impact soil organic matter has on nutrient availability. Multiplying the percent organic matter by the value of the nutrient made available results in the value of the fertility in the soil. Specific values of nutrient will vary.
  • Soil organic matter increases water management. Both drought and flood conditions are ameliorated. The amount of change depends on soil type, but going from 1 to 5% SOM doubles the water holding capacity. Specific value of the $/% will vary by delivered cost of water. This is an additional benefit of soil organic matter.
  • Soil Analysis reports are an important tool in managing nutrient removal and replacement.
  • Soil solution is the aqueous liquid phase of the soil and its solutes (dissociated ions). Nutrients occur in the soil solution mostly as ions: NH4+, N03-, H2P04-, HP042-, K+, Ca2+, Mg2+, S042-, Fe2+, Mn2+, Zn2+, Cu2+, Mo042-, H2B03-, C1-.
  • Roots of higher plants can only take up nutrients from the soil solution. Uptake by plants and leaching by rain or irrigation depletes the amount of ions in solution unless they are replenished from reserves contained in primary minerals and organic matter and attached to colloids. Some processes of release are rapid (e.g. cation exchange), while others are slow with rates of release more critical (e.g. release of N, S and sometimes P by the decay of soil organic matter). The concentration of nutrients in solution and their rates of release depend upon the amount and types of solid-phase nutrients present in the soil, soil water content, temperature, aeration, microbial activity, and other properties of the soil solution.
  • PGPR Disease control by PGPR has attracted much attention in reducing plant diseases. Although the full potential has not been reached yet, the work to date is promising and offer growers some of their first sustainable effective control of serious plant diseases. Some PGPR, especially if they are inoculated on the seeds before planting, are able to establish themselves on the crop roots. They use scarce resources, and thereby prevent or limit the growth of pathogenic microorganisms. Even if nutrients are not limiting, the establishment of beneficial organisms on the roots limits the chance that pathogenic organisms that arrive later will find space to become established. Rhizosphere organisms are capable of producing compounds that are toxic to pathogens.
  • Soil friability involves cation balance, a vital, diverse soil- life community and humus.
  • Cation balance refers to the relative percentage of the key, positively charged minerals (cations) that are attached to the clay in soil.
  • Clay is negatively charged and it serves as the main storage system for calcium, magnesium, potassium and sodium. It has been determined that there is a productive balance of these major cations on the clay colloid and the ratio between these cations has a big influence on how well a soil can breathe. The most important ratio is the Ca:Mg ratio.
  • Calcium opens (flocculates) the soil because it is a large ion with two charges that pushes apart the clay colloids and holds them apart. Magnesium also has two charges that can stick clay colloids either side of the ion.
  • This mineral however is much smaller than calcium and if there is too much magnesium in relation to calcium, the soil will be tight and closed.
  • a high magnesium soil is not conducive to fostering, aerobic soil life because a lack of oxygen becomes the limiting factor.
  • the ideal calcium to magnesium ratio in a heavy clay soil might be 7: 1 while in a light soil that might be 3: 1 (in favor of calcium).
  • magnesium mineral In light sandy soils, we actually want relatively more of the soil tightening, magnesium mineral to give a little more structure.
  • Soil life is the other determinant of good gas exchange. Soil bacteria, in their billions, exude a sticky substance than binds together soil particles to create a mini aggregate. Fungi grab these particles with their tentacle-like strands (hyphae) and mold them into larger soil crumbs and this creates crumb structure, the most desirable of all soil conditions. Earth worms and beneficial nematodes can move freely through these soils, conferring all of their associated benefits. Plant roots, and the mycorrhizal fungi attached to them, can grow unimpeded. Moisture moves easily in, from above and below (the water table). The higher the humus levels of soils, the greater the likelihood of crumb structure as humus is both the home base and manifestation of the soil creatures that confer crumb structure.
  • adjuvant - material added to enhance action in this case surfactant, fulvic acid, and enzymes, enhance absorption, transportation, and plant functions.
  • “Brix” is a measure to quantify the amount that light is bent passing through a fluid. It is a tool to determine the value of crops. The measurement is based on the percent solids in a given weight of, e.g., plant juice.
  • Biopesticides are pesticides made from natural materials and for the use herein do not harm microbes.
  • Comppatible means that it does not harm the microbes. It can be in the same container, but not necessarily. It may be co-applied. That is supplied in separate containers, but applied together. It may be applied separately and separate times.
  • Fulvic acids are humic acids of lower molecular weight and higher oxygen content than other humic acids. Fulvic acids are poly-electrolytes and are unique colloids that diffuse easily through membranes whereas all other colloids do not.
  • mycoplasma and parasites that affect soil and/or plant health.
  • Micronutrients CI, Fe, B, Mn, Zn, Cu, Mo, & Ni.
  • PFLA phospholipid fatty acids
  • Rhizobacteria Plant Growth Promoting Rhizobacteria is a number of benificial microbs found in healthy Rhizosphere of a plant.
  • Plants include seeds, seedlings, transplants and crops growing in fields.
  • Example are provided for illustrative purposes and are not intended to limit the scope of the disclosure.
  • EXAMPLE 1 PROVIDE A GLOBAL SOIL SOLUTION.
  • Product - A complete nutritive formula with macro, and micro nutrients and complementary enzyme microbial soil formulation.
  • a service customizes the product based on soil analysis and the structure, chemistry, microbial activity of the soil. If necessary tissue analysis pathology are performed. The expertise is included to interpret, customize, and deliver customized treatment.
  • a follow up program measures the results and adjusts the program to maximize results.
  • a goal is to make a unique plant nutrient formula that addresses the macro, and micro nutrients that a plant needs.
  • the formula can be applied to foliar, soil, in-furrow while seeding, seedling root dip, or hydroponically.
  • EXAMPLE 2 RESPONSE TO HLB PROBLEM (Huanglongbing, “citrus greening disease")
  • the application of microbes, compatible nutrients that are in the form readily absorbed and not harsh (will not burn) has show dramatic results.
  • the HLB management process includes:
  • Nitrogen in the form of amino acids which supplies energy to the seedling in a non-burning readily metabolized form.
  • CEC Cation Exchange Capacity
  • Fulvic acid a low molecular weight subcomponent of Humic acid, aids in the absorption and transmigration of water and nutrient through the cell walls in the rapidly growing cells of the seedlings.
  • Soil testing is done to set a baseline and prescribe customization of future applications. Trees and soil are inoculated during transplanting with microbial and nutrient formulas in order to help ensure minimal stress from replanting and ensure optimal fertile growing conditions in the immediate soil. Improvement in Cation Exchange Capacity (CEC), organic matter and water address the parched soil and enhance water retention. All together address the symbiosis of microbes, and
  • Inoculation is done using microbes, macro and micro nutrients which help in germination and rapid growth and some or all the following:
  • Bio-char may be partially prefilled in holes to increase carbon in the soil.
  • B. Surfactants which break surface tension to improve water penetration and retention in soil.
  • C. Nitrogen in the form of amino acids which supplies energy to the seedling in a non burning readily metabolized form.
  • Humic acid increases organic matter supporting water retention
  • a breakthrough for implementing soil fertility at the nursery came from the need to be able to reach growers at a stage when they would be most receptive. At the seed and seeding stage there is little material required, but the rapid growth make great venue for demonstration of the improved efficacy of the system.
  • This product and crop management system can be put in-furrow.
  • the microbes inoculate the seed and soil. Microbes improve germination. Microbes inoculate the soil by balancing the microbial ecology that may be dormant or absent after a winter or fallow period.
  • the hearty microbe ecology with continue to grow with the plant, in the plant, in the rhizosphere.
  • Application of the benign plant available nutrients provides full spectrum balanced nutrition.
  • Humic acid is a concentrate of organic matter provides food for microbes and plant.
  • the complimentary mix restores the fertility of the soil directly where the seed is planted.
  • In-furrow application can be effective in till applications. This is ideal in a no till application. It can take several years for the plant matter to degrade.
  • the applied microbe and benign nutrients accelerate this process. While waiting the in-furrow using a disk or seed drill application causes the lease disturbance. In both till and no-till application this method is efficient by placement of material applied at seeding in-furrow.
  • EXAMPLE 3 FORMULATION MODIFICATION BY NUTRITIONAL REMOVAL. Nutrient Removal. In the case shown in Figure 4 the soil test indicates excessive copper. This was due to Copper applied to treat Canker in Florida (Cu). The formula for the nutrient mixture has copper removed (see FIG. 4)
  • Purpose May enhance micronutrient uptake.
  • Purpose May enhance micronutrient uptake.
  • Purpose May enhance micronutrient uptake.
  • Chelated Zinc Derived from Di-Potassium Phosphate, Potassium, Citrate, Copper Citric Acid, Iron Citric Acid, Manganese, Citric Acid, Boric Acid, and Sodium Molybdate.
  • Purpose May enhance micronutrient uptake.
  • Purpose May enhance micronutrient uptake.
  • EXAMPLE 4 NUTRIENT ADDITION.
  • the balancing of the calcium and magnesium ratio is a common need. This can be done be adding to a Humic and kelp solution in the C-HumiCHARGETM for the addition of calcium or M- HumiCHARGETM for the addition of magnesium. Similarly for any of the macro- or micro- nutrients.
  • K-HumiCHARGE Potassium consisting of 1% kelp, (6-1-8) )"NPK" 6%
  • TABLE 3 shows soil chemistry test results before and after one application; the biodiversity and quantity were improved by the test results; the soil chemi.Ostry also showed improvement.
  • Micronutrients CI, Fe, B, Mn, Zn, Cu, Mo, & Ni.
  • the soil is the stomach of the plant.
  • the rhizosphere - the root zone there is a symbiotic relationship between the bacterial (consuming the nutrients such as nitrogen for minerals or the air.) protozoa which attack the bacterial and release nutrients (such as nitrogen) and Fungi.
  • protozoa which attack the bacterial and release nutrients (such as nitrogen) and Fungi.
  • Enzymes are chemical accelerators for molecular reactions. Enzymes have
  • Microbes produce enzymes and perform complexing, are regenerative, endure wider range of temperature, moisture, for extensive periods of time.
  • App Use of computer and smart phone application tool can help present, track and connect data, facilitate monitor and verify soil fertility.
  • the present invention provides an isolated Rhizobium phaseoli CA bacterial strain.
  • the present invention provides an isolated Bradyrhizobium japonicum CA
  • the present invention provides an isolated Rhizobium meliloti FD bacterial strain.
  • the present invention provides an isolated Paenibacillus brasiliensis 172 bacterial strain.
  • said microbial isolate is selected from the group consisting of a bacterial isolate and a fungal isolate.
  • said fungal isolate is selected from the group consisting of a Trichoderma virens 3107 fungal strain, a Trichoderma viride G fungal strain, a Trichoderma viride LK fungal strain, a Trichoderma harzianum 3147 fungal strain, a Trichoderma harzianum G fungal bacterial strain, a Trichoderma harzianum LK fungal strain, a Trichoderma longibrachiatum 3108 fungal strain.
  • said bacterial soil isolate is selected from the group consisting of a Bacillus sp.
  • RG-S bacterial strain an Ensifer meliloti FD bacterial strain, a Rhizobium trifolii FD bacterial strain, an Azorhizobium caulinodans KN bacterial strain, a Rhizobium sp. RLGl bacterial strain, an Azorhizobium sp. RLG2 bacterial strain, an Azorhizobium sp. RLG3 bacterial strain, a Rhizobium (sp. RLG4 bacterial strain, a Rhizobium sp. RLG5 strain, a Rhizobium sp. RLG6 bacterial strain. Azorhizobium sp. RLG7 bacterial strain, a Rhizobium sp.
  • RLG8 bacterial strain an Azorhizobium sp. RLG9 bacterial strain, a Rhizobium sp. RLG10 bacterial strain, a Rhizobium sp. RLGl 1 bacterial strain, a Bacillus sp. LK bacterial strain, a Pseudomonas fluorescens CA bacterial strain, an Azospirillum CA bacterial strain, an Acetobacter sp.
  • said microbial soil isolate is selected from the group consisting of a Bacillus sp.
  • RG-S bacterial strain an Ensifer meliloti FD bacterial strain, a Rhizobium trifolii FD bacterial strain, an Azorhizobium caulinodans KN bacterial train, a Rhizobium sp. RLGl bacterial strain, an Azorhizobium sp. RLG2 bacterial strain, an Azorhizobium sp. RLG3 bacterial strain, a hizobium sp. RLG4 bacterial strain, a Rhizobium sp. RLG5 strain, a Rhizobium sp. RLG6 bacterial strain. Azorhizobium sp. RLG7 bacterial strain, a Rhizobium sp.
  • RLG8 bacterial strain an Azorhizobium sp. RLG9 bacterial strain, a Rhizobium sp. RLGIO bacterial strain, a Rhizobium sp. RLG1 1 bacterial strain, a Trichoderma virens 3107 fungal strain, a Trichoderma viride LK fungal strain, a Trichoderma viride 31 16 fungal strain, a Trichoderma harzianum 3147 fungal strain, a Trichoderma harzianum G fungal strain, a Trichoderma harzianum LK fungal strain, a Trichoderma longibrachiatum 3108 fungal strain, a Bacillus sp.
  • LK bacterial strain a Pseudomonas fluorescens CA bacterial strain, an Azospirillum CA bacterial strain, an Acetobacter sp. LK bacterial strain.
  • Rhizobium phaseoli CA bacterial strain a Bradyrhizobium japonicum bacterial strain, a Rhizobium meliloti FD bacterial strain, a Paenibacillus brasiliensis 172 bacterial strain , a Paenibacillus peoriae bacterial strain having accession number BD-62, a Paenibacillus polymyxa bacterial strain having accession number B37-A.
  • said microbial soil isolate is selected from the group consisting of a Bacillus sp.
  • LK bacterial strain an Bacillus subtilis LK bacterial strain, a Rhizobium trifolii FD bacterial strain, an Azorhizobium caulinodans KN bacterial train, a Pseudomonas fluorescens bacterial strain, an Azospirillum CA bacterial strain, an Acetobacter sp.
  • said microbial formulation further comprises a carrier, such that the microbial formulation of the present inventions are delivered to a seed or plant in a manner to promote growth and productivity, such as germination, yield, and the like. It is not meant to limit the type of carrier.
  • a variety of carriers are contemplated including but not limited to a liquid, a solid and a combination of a liquid and a solid carrier.
  • the carrier is a liquid comprising water.
  • a carrier comprises a microbial growth medium.
  • a carrier further comprises humic acid, minerals, artificial compounds, particles, such as beads, powders or granules, and the like.
  • a particle comprises a resin, clay, a biodegradable compound, and the like.
  • a bead comprises polymethyl methacrylate (PMMA) (end of excerpt from U.S. 8,822, 190).
  • PMMA polymethyl methacrylate

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Biotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

L'invention concerne une composition comprenant des macronutriments, des micronutriments, des enzymes et des micro-organismes qui améliore la fertilité des sols et favorise la santé et le rendement des plantes, appropriée pour une utilisation sur des cultures sur pied, des greffes, des graines et des semis de pépinière. L'invention concerne en outre un liquide de pulvérisation des végétaux compatible avec, et complémentaire des micro-organismes et nutriments de la composition, un produit de nettoyage efficace pour aider à nettoyer et améliorer le fonctionnement d'un équipement de pulvérisation ainsi qu'un kit comprenant des contenants de produits de nettoyage, de microbes, nutriments et/ou pesticides, des descriptions et des instructions d'utilisation. Des moyens pour distribuer les compositions sous forme liquide et/ou sous forme sèche pour un usage agricole ou par le consommateur. L'invention concerne également des procédés pour améliorer la fertilité du sol dans un sol cible et pour réduire les coûts des agriculteurs, des cultivateurs et des éleveurs.
PCT/US2015/051025 2014-09-19 2015-09-18 Compositions, procédés, conditionnement et appareil pour améliorer la fertilité des sols et la croissance des plantes WO2016044768A1 (fr)

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US62/052,829 2014-09-19
US201562106345P 2015-01-22 2015-01-22
US62/106,345 2015-01-22

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CN107298608A (zh) * 2017-06-27 2017-10-27 刘磊 一种液体硼肥的制作方法
WO2018187345A1 (fr) * 2017-04-03 2018-10-11 Spogen Biotech Inc. Compositions agricoles pour une productivité améliorée des cultures et des phénotypes renforcés
CN108834816A (zh) * 2018-05-24 2018-11-20 西藏俊富环境恢复有限公司 一种高海拔地区苗木移栽方法
CN109400379A (zh) * 2018-12-07 2019-03-01 陈德彩 用于园林绿化的驱虫复合肥及其制备方法
CN110452024A (zh) * 2019-09-06 2019-11-15 哈尔滨工业大学 一种利用微生物发酵废料制备的水稻育苗床土及其制备方法
KR20200011504A (ko) * 2020-01-14 2020-02-03 주식회사 지네트코리아 잔디 식재방법
US11560339B2 (en) 2019-05-30 2023-01-24 Koch Agronomie Services, LLC Micronutrient foliar solutions
WO2023036938A1 (fr) 2021-09-10 2023-03-16 Basf Se Micro-organismes de production d'exopolysaccharides et leurs utilisations

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018187345A1 (fr) * 2017-04-03 2018-10-11 Spogen Biotech Inc. Compositions agricoles pour une productivité améliorée des cultures et des phénotypes renforcés
CN107298608A (zh) * 2017-06-27 2017-10-27 刘磊 一种液体硼肥的制作方法
CN108834816A (zh) * 2018-05-24 2018-11-20 西藏俊富环境恢复有限公司 一种高海拔地区苗木移栽方法
CN108834816B (zh) * 2018-05-24 2020-07-07 西藏俊富环境恢复有限公司 一种高海拔地区苗木移栽方法
CN109400379A (zh) * 2018-12-07 2019-03-01 陈德彩 用于园林绿化的驱虫复合肥及其制备方法
US11560339B2 (en) 2019-05-30 2023-01-24 Koch Agronomie Services, LLC Micronutrient foliar solutions
CN110452024A (zh) * 2019-09-06 2019-11-15 哈尔滨工业大学 一种利用微生物发酵废料制备的水稻育苗床土及其制备方法
CN110452024B (zh) * 2019-09-06 2021-11-02 哈尔滨工业大学 一种利用微生物发酵废料制备的水稻育苗床土及其制备方法
KR20200011504A (ko) * 2020-01-14 2020-02-03 주식회사 지네트코리아 잔디 식재방법
KR102117390B1 (ko) 2020-01-14 2020-06-01 주식회사 지네트코리아 잔디 식재방법
WO2023036938A1 (fr) 2021-09-10 2023-03-16 Basf Se Micro-organismes de production d'exopolysaccharides et leurs utilisations

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