WO2016043177A1 - Natural cheese exhibiting heat-resistant shape retention and method for manufacturing said cheese - Google Patents

Natural cheese exhibiting heat-resistant shape retention and method for manufacturing said cheese Download PDF

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Publication number
WO2016043177A1
WO2016043177A1 PCT/JP2015/076105 JP2015076105W WO2016043177A1 WO 2016043177 A1 WO2016043177 A1 WO 2016043177A1 JP 2015076105 W JP2015076105 W JP 2015076105W WO 2016043177 A1 WO2016043177 A1 WO 2016043177A1
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Prior art keywords
cheese
transglutaminase
heat
natural cheese
shape retention
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PCT/JP2015/076105
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French (fr)
Japanese (ja)
Inventor
兼一 城ノ下
素晴 小森
武志 渡邉
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株式会社明治
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Priority to JP2016548890A priority Critical patent/JP6671289B2/en
Publication of WO2016043177A1 publication Critical patent/WO2016043177A1/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/06Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068Particular types of cheese

Definitions

  • the present invention relates to natural cheese having heat-resistant shape retention and a method for producing the same.
  • Natural cheese is becoming established in the Japanese market. Natural cheese can be classified according to the degree of ripening into so-called mature natural cheese that can enjoy the ripening flavor of milk components, and so-called non-ripened natural cheese that can taste a fresh milk flavor. Natural cheese can be classified into special hard natural cheese, hard natural cheese, semi-hard natural cheese, and soft natural cheese based on its hardness. Thus, there are many types of natural cheese depending on the degree of ripening (presence / absence) and the physical properties (texture).
  • Natural cheese may be eaten as it is as a food scene, but it may be eaten as it is cooked and processed using the characteristics of cheese.
  • the characteristic that the cheese melts by heating and increases the stringiness is actively utilized.
  • fried foods such as frying and tempura, fried foods, ingredients for baked goods, etc.
  • the characteristics that the cheese melts by heating and increases the stringiness are not required.
  • the cheese is not easily melted by heating, and heat-resistant shape retention that can maintain the original shape to some extent is required.
  • Patent Document 1 discloses a cheese melt prepared by melting natural cheese or processed cheese as a main raw material and using water, molten salt, and optionally various additives such as milk-derived proteins, seasonings and flavors. It is characterized by having transglutaminase act on the product, having moderately both melting deformation (easy melting) and spinnability by heating, or no melting deformation by heating (heat-resistant shape retention), crisp A method for producing a cheese food having a good crumb-like structure is described.
  • Natural cheese generally has a natural milk flavor and unique structure and properties, and consumers who prefer such natural cheese structure and properties may not be satisfied with processed cheese or cheese food. .
  • natural cheese having heat resistance for example, Cyprus harrows are known.
  • harrowmi is produced by heating raw material milk in a high-temperature whey at about 90 ° C. while maintaining a neutral pH without using lactic acid bacteria.
  • milk protein concentrate powder is dissolved in water, raw milk, or skim milk, and after adding milk fat, homogeneous treatment is performed to prepare raw milk, and this raw milk is mixed with lactic acid bacteria starter or acidic
  • lactic acid bacteria starter or acidic A natural cheese characterized by having heat resistance and water resistance, which can be obtained by adding curatives and curdling enzymes to form curds is described.
  • the cheese food of Patent Document 1 lacks the natural milk flavor and original structure and physical properties of the original natural cheese. Moreover, in the manufacturing process of the harrow which is the natural cheese which has the said heat-resistant shape retention property, a dedicated heating apparatus is essential in addition to the normal natural cheese manufacturing apparatus. For this reason, the manufacturing process becomes complicated, and it is necessary to newly invest in facilities, so that it is not easy to put it to practical use as commercial mass production. In addition, hallowmi does not have a fermentation process with lactic acid bacteria, and the pH of the raw milk remains neutral. Therefore, in view of hygiene, etc., salt is added until the total salt content is 3 to 4% by weight. There is a need to.
  • the salt content becomes high, and there is a concern about the health effect of excessive intake of salt. Furthermore, in the production process of harrow, there is no aging process with lactic acid bacteria, and so-called non-ripe-molded natural cheese, so that the flavor of aging of milk components cannot be enjoyed.
  • Patent Documents 3 to 5 describe a method for suppressing separation of whey by adding transglutaminase before curling the raw milk or simultaneously with curling the raw milk in preparing natural cheese. Has been. However, according to the present inventors, this method generates whey having a quality different from that of the prior art, which makes the whey treatment complicated, causes water separation during aging, and has a poor texture. Problems such as becoming are found.
  • the present invention has an object to provide natural cheese having heat-resistant shape retention and a method for producing the same without impairing the original flavor and composition of natural cheese.
  • the present inventors added (mixed) transglutaminase to the cheese curd of natural cheese after discharging the whey after curling the raw milk. As a result, it was found that natural cheese having heat-resistant shape retention can be produced by aging, and the present invention was completed.
  • natural cheese having heat-resistant shape retention and the method for producing the same of the present invention natural cheese having heat-resistant shape-retaining property can be realized without adjusting the composition of moisture and milk protein in the raw milk.
  • the process until discharging whey can adopt the same process as the normal (conventional) production method, and whey generated by curding raw material milk of the present invention is: Since it has the same physical properties and quality (same amount, same composition, etc.) as whey generated by the production method of ordinary natural cheese, there is no problem that the process of reusing whey is complicated. Further, in the production method of the present invention, normal whey having the same quality as the conventional one is generated, so that problems such as water separation during aging and poor texture do not occur.
  • the present invention relates to the following.
  • a method for producing natural cheese having heat-resisting shape-retaining properties comprising curling raw material milk, preparing whey and preparing cheese curd, adding transglutaminase to the cheese curd and aging.
  • the production method according to any one of (1) to (3) above which does not include heating at a temperature and a time at which transglutaminase is deactivated after adding transglutaminase.
  • Natural cheese having heat-resistant shape retention produced by the production method according to any one of (1) to (5).
  • the natural cheese according to (6) which has shape-retaining properties at 150 to 250 ° C., 5 to 20 minutes of dry heat heating and / or 100 to 200 ° C. and 5 to 40 minutes of wet heat heating.
  • It has a shape retention of 50% or more in dry heat heating at 150 to 250 ° C. for 5 to 20 minutes and / or a shape retention of 40% or more in wet heat heating at 100 to 200 ° C. for 5 to 40 minutes.
  • (11) The natural cheese according to any one of (6) to (8), which is for cooked food.
  • the present invention is as follows.
  • [1] Manufacture of natural cheese having heat-resistant shape retention, characterized in that after curding raw milk, whey is discharged to prepare a cheese curd, and transglutaminase is added to the cheese curd and ripened.
  • Method. [2] A method for producing natural cheese having heat-resistant shape-holding properties according to the above [1], wherein a milk-clotting enzyme and lactic acid bacteria are added and allowed to curd up.
  • [3] The method for producing natural cheese having heat-resistant shape-holding properties according to [1] or [2], wherein the natural cheese is a mature molded natural cheese,
  • Natural cheese is baked under dry heat for 5 to 20 minutes at 150 to 250 ° C.
  • Natural cheese is baked under dry heat for 5 to 20 minutes at 150 to 250 ° C. and has a shape retention of 50% or more, and / or 5 to 40 at 100 to 200 ° C.
  • a natural cheese having heat-resistant shape retention produced by the production method according to the above [1] to [6].
  • whey is discharged to prepare a cheese curd, and transglutaminase is added to the cheese curd and matured, and cooking and processing by heating in a frying pan or oven, etc.
  • transglutaminase is added to the cheese curd and matured, and cooking and processing by heating in a frying pan or oven, etc.
  • natural cheese refers to milk, buttermilk, skimmed milk, cream or a mixture of these, or from a coagulated milk obtained by coagulating a part of protein with an enzyme or other coagulant. This is the product from which the part has been removed or the product that has been aged.
  • raw milk means milk used as a raw material, and typically refers to raw milk that has not been sterilized, and uses animal milk such as cow's milk, sheep milk, buffalo milk, and goat milk. Can do. And in this invention, the composition of raw material milk can be adjusted according to the natural cheese actually made into the objective.
  • skim milk and cream can be separated from raw milk that has not been sterilized using a cream separator or the like, and the skim milk and cream can be blended in various mixing ratios to adjust the content of milk fat.
  • minerals, vitamins, lactose, milk protein, milk fat, and the like can be separated using a known separation membrane and the like, and these components can be blended in a mixing ratio to adjust the content of these components.
  • butter milk, skim milk, cream, etc. can be mixed with raw milk, and the content of milk fat and the content of non-fat milk solids can also be adjusted.
  • the content of milk fat in raw milk can be adjusted.
  • the milk fat content of the raw milk can be expressed as the ratio of the milk fat content in the total solid content of the raw milk. For example, if the proportion of milk fat in the total solid content of raw material milk is low, it becomes a so-called low fat type natural cheese, and if the proportion of milk fat in the total solid content of raw material milk is high, it is a so-called high fat type. Natural cheese.
  • the ratio of the milk fat content in the total solid content of the raw material milk is not particularly limited, but for example, 0 to 80% by weight, 0 to 70% by weight, 0.5 to 65% by weight, 0.5 to 60% by weight, 1-55 wt%, 2-55 wt%, 5-55 wt%, 10-55 wt%, 15-55 wt%, 20-55 wt%, 25-55 wt%, 30-55 wt%, 35- 55% by weight.
  • the ratio of the milk fat content in the total solid content of the raw material milk is 0% by weight.
  • the lower limit of the ratio of milk fat content in the total solid content is 0.5% by weight, preferably 1% by weight, more preferably 2% by weight, still more preferably 5% by weight, and the upper limit is 20% by weight, preferably Is 15% by weight, more preferably 10% by weight, still more preferably 5% by weight.
  • the lower limit value and the upper limit value of the ratio of the milk fat content in the total solid content of the raw material milk can be set in appropriate combination.
  • the lower limit of the ratio of milk fat content in the total solid content of raw milk is 20% by weight, preferably 25% by weight, more preferably 30%.
  • the upper limit is 80% by weight, preferably 70% by weight, more preferably 65% by weight, still more preferably 60% by weight, and still more preferably 55% by weight.
  • the lower limit value and the upper limit value of the ratio of the milk fat content in the total solid content of the raw material milk can be set in appropriate combination.
  • raw material milk may be sterilized by a generally known raw material sterilization method.
  • the raw milk is sterilized by a pasteurization method used in a generally known natural cheese manufacturing method
  • the lipase present in the raw milk before sterilization is deactivated, and before sterilization
  • the number of viable bacteria such as general bacteria present in the raw milk can be reduced, and this is preferable in producing natural cheese with good quality.
  • curd milk means to harden raw material milk and includes an action of forming a generally known cheese curd.
  • a well-known method can be used in the method of coagulating raw material milk.
  • a method of adding (compounding) a curdling enzyme such as rennet to raw milk a method of heating after adjusting the pH of raw milk, adding lactic acid bacteria to raw milk, fermenting with lactic acid bacteria, A method of lowering the pH and aggregating the raw milk can be used.
  • a method of adding (compounding) a curdling enzyme such as rennet to raw milk a method of heating after adjusting the pH of raw milk, adding lactic acid bacteria to raw milk and fermenting with lactic acid bacteria
  • a curdling enzyme such as rennet
  • the lactic acid bacterium may be any lactic acid bacterium that can be used in natural cheese, and its genus and species are arbitrary, and examples thereof include known lactic acid bacteria such as Lactis, Cremoris, Bulgaria, and Thermophilus. Can do.
  • “cheese curd” refers to a product obtained by discharging all or part of whey separated by curdling raw material milk.
  • a method for discharging whey a method used in a generally known method for producing natural cheese can be used.
  • the whey refers to, for example, a product obtained by separating a coagulated product produced by curdling raw material milk, and refers to water containing whey protein and the like.
  • “to discharge whey” means, for example, to discharge whey from a state where coagulated material and whey produced by curdling raw material milk coexist.
  • the present invention is characterized in that transglutaminase is added to cheese curd from which whey has been discharged after the raw milk has been coagulated. Therefore, in this discharged whey, the amount and composition of whey produced by the production of generally known natural cheese other than the present invention are the same.
  • Whey can be processed well.
  • a method for processing whey a known method can be used. For example, a method of processing into whey powder, desalted whey powder, whey protein concentrate (WPC), whey protein isolate (WPI), permeate, delactose permeate, or lactose can be used.
  • Transglutaminase generally catalyzes an acyl transfer reaction between a ⁇ -carboxamide group of a glutamine residue in a protein or peptide chain and a primary amine, and if the primary amine is a lysine residue of the protein, ⁇ - It is known to have an effect of forming ( ⁇ -Glu) -Lys crosslinks.
  • transglutaminase In natural cheese with transglutaminase added and allowed to act on transglutaminase, transglutaminase has been added to raw milk so far to improve the yield based on raw milk by suppressing the separation and discharge of whey. It was known. In contrast, in the present invention, whey is intentionally separated and discharged, transglutaminase is added to the cheese curd after the whey is discharged, and transglutaminase is allowed to act in the subsequent ripening step, thereby allowing the conventional natural While maintaining the composition (for example, water content) and quality of cheese, the heat resistant shape retention of natural cheese could be improved.
  • the composition for example, water content
  • the general method for producing natural cheese is: (1) a step of agglomerating raw milk to produce a coagulum and whey; (2) cutting the coagulum and separating whey from (inside) the coagulum, (3) including a process of discharging whey.
  • transglutaminase has been used before whey is discharged, that is, immediately before or after the above step (1) or (2).
  • transglutaminase is used after discharging whey, that is, after the step (3).
  • the process of ripening a cheese curd is included after the said process (3). Therefore, in the present invention, in order to produce ripened natural cheese, transglutaminase is used before the step of ripening the cheese curd.
  • the step of ripening the cheese curd is completed, if it includes a step of heating at such a temperature and time that the transglutaminase is deactivated, in the matured natural cheese of the final product or intermediate product, transglutaminase This is because even when the activity does not remain, transglutaminase acts sufficiently during the ripening period, and heat-resistant shape retention is imparted well in the final product and the intermediate product of mature cheese.
  • the completion of the process of ripening the cheese curd means a stage where a desired ripening flavor is obtained in the mature natural cheese of the final product or intermediate product.
  • heating at a temperature and time at which transglutaminase is inactivated means that the conditions vary depending on the type and state of transglutaminase, which can be easily determined by product information or normal experiments in the art. I can understand.
  • KS-CT manufactured by Ajinomoto Co., Inc.
  • it is deactivated by heating at 65 ° C. for 2 hours or more, and for heating at a higher temperature for a short time, for example, heating at 72 ° C. or more for 15 seconds or more. Deactivated.
  • transglutaminase has the action of transglutaminase
  • its origin, enzyme activity, and degree of purification can be arbitrarily selected and used.
  • a commercially available transglutaminase preparation can be used.
  • transglutaminase in the method of adding transglutaminase to cheese curd, transglutaminase may be dispersed or mixed substantially uniformly in cheese curd, and its specific means is not limited, but for example, in cheese curd grains After adding high-concentration transglutaminase, stirring in a tank (cheese vat), etc., spraying high-concentration transglutaminase onto cheese curd grains, in-line with cheese curd grains and high-concentration transglutaminase ( Generally known dispersion or mixing methods such as a method of flowing in a pipe) can be used.
  • transglutaminase is preferably added after forming cheese curd.
  • transglutaminase is added before the raw milk is curd, or when transglutaminase is added while the raw milk is curding, when forming the cheese curd. This is because the whey is not sufficiently separated and discharged from the cheese curd, water separation occurs in the process of aging the cheese curd, and it is difficult to ensure the original quality of natural cheese.
  • transglutaminase is added to the obtained cheese curd grains.
  • the pH of the whey reaches 5.8 to 6.4, preferably 5.9 to 6.3, more preferably about 6.0 to 6.2. Is discharged.
  • the amount of transglutaminase added is arbitrary, and can be easily adjusted by information on products or a normal experiment in the technical field.
  • the lower limit of the amount of transglutaminase added can provide the effect of the present invention.
  • the transglutaminase concentration is 0.0002 wt% or more, 0.0003 wt% or more, 0.0004 wt% or more, 0.0005 wt% or more 0.0006 wt% or more, 0.0007 wt% or more, 0.0008 wt% or more, 0.0009 wt% or more, 0.001 wt% or more, 0.0012 wt% or more, 0.0014 wt% or more, It is preferably 0.0016% by weight or more, 0.0018% by weight or more, or 0.002% by weight or more.
  • the lower limit value of the addition amount of transglutaminase is preferably the above-described high numerical value.
  • the upper limit of the amount of transglutaminase added is the effect of the present invention.
  • the transglutaminase concentration is 10% by weight or less, 5% by weight or less, 1% by weight or less, 0.1% by weight or less, 0.05 % By weight or less, 0.01% by weight or less, 0.008% by weight or less, 0.006% by weight or less, 0.005% by weight or less, 0.004% by weight or less, 0.003% by weight or less.
  • the amount of transglutaminase added is too large, natural cheese tends to become very hard, so in addition to the effect of heat-resistant shape retention, the addition of transglutaminase
  • the upper limit of the amount is preferably the above-mentioned low numerical value.
  • the amount of transglutaminase added is the cheese curd weight ratio (curd grain ratio).
  • the transglutaminase concentration is 0.0002 to 10% by weight, 0.0003 to 10% by weight, 0.0004 to 5% by weight, 0.0005 to 5% by weight, 0.0006 to 1% by weight.
  • % 0.0007 to 1% by weight, 0.0008 to 0.1% by weight, 0.0009 to 0.05% by weight, 0.001 to 0.01% by weight, 0.0012 to 0.008% by weight, 0.0014 to 0.006 wt%, 0.0016 to 0.005 wt%, 0.0018 to 0.004 wt%, 0.002 to 0.003% wt% There it is preferable.
  • the cheese curd can be salted as necessary.
  • the time for salting the cheese curd may be the same as the time for adding transglutaminase to the cheese curd, or after adding the transglutaminase to the cheese curd and before ripening by shaping the cheese curd.
  • the method of salting a cheese curd can use well-known methods, such as the method of immersing in a brine (brine).
  • a matured natural cheese can be produced by providing a step of aging a cheese curd.
  • ripening a cheese curd means aging in normal natural cheese, and normal known conditions in a method for producing a mature natural cheese can be used.
  • the known conditions for mature natural cheese are, for example, that the temperature is kept at 4 to 20 ° C. and the humidity is kept at 50 to 80%.
  • the well-known conditions in the manufacturing method of the matured natural cheese actually made into the objective can be used, for example, suitable temperature, such as 4 degreeC, 7 degreeC, 15 degreeC, and 20 degreeC, is a lower limit. And the upper limit value can be selected and set.
  • the well-known conditions in the manufacturing method of the matured natural cheese actually intended can be used, for example, appropriate humidity, such as 50%, 60%, 70%, 80%, 90%, etc.
  • appropriate humidity such as 50%, 60%, 70%, 80%, 90%, etc.
  • Each of the lower limit value and the upper limit value can be selected and set.
  • the period for ripening the cheese curd is not particularly limited, but the action of transglutaminase is sufficiently exerted to improve the heat-resistant shape retention of natural cheese. From the viewpoint, it is 1 day or more, preferably 2 days or more, more preferably 1 week or more, still more preferably 2 weeks or more, and further preferably 1 month or more.
  • the period which ripens a cheese curd is 1 year or more from the viewpoint of obtaining the ripening flavor of natural cheese, Preferably it is 1.5 years or more, More preferably, it is 2 years or more, More preferably, it is 3 years or more.
  • the ripening period of the cheese curd is not particularly limited as long as the heat resistant shape retention which is the effect of the present invention can be obtained, but specifically, 1 day to 2 years, 2 days to 1 year, It is preferable that the period is from week to 6 months, from 2 weeks to 4 months, and from 1 month to 4 months. That is, in this invention, the conditions and period used by the manufacturing method of a general natural cheese can be employ
  • the present invention can be applied to all natural cheeses that require a ripening process. That is, the present invention can be exemplified by mature cheeses such as gouda, cheddar and parmesan, white mold cheeses such as camembert and brie, and blue mold cheeses such as gorgonzola and roquefort, but natural cheeses which require a ripening step other than those exemplified above. It can be applied to all of the above. Therefore, the manufacturing method of the natural cheese of this invention can be used for the manufacturing method of the natural cheese which requires a ripening process.
  • mature cheeses such as gouda, cheddar and parmesan
  • white mold cheeses such as camembert and brie
  • blue mold cheeses such as gorgonzola and roquefort
  • the natural cheese of the present invention can be added with any food material and / or food additive as necessary, taking into consideration the design of the product, as long as it does not significantly affect the heat-resistant shape retention.
  • the natural cheese of the present invention has heat-resistant shape retention.
  • This measurement method for heat-resistant shape retention can use two types of firing (heating) under dry heat and wet heat.
  • heat-resistant shape retention during baking under dry heat (during dry heat heating)
  • an aluminum foil is laid on an aluminum tray, and natural cheese is cut out and installed.
  • room temperature about 20 degreeC
  • heat-resistant shape retention of baking under wet heat (at the time of wet heat heating)
  • filter paper is spread on the petri dish, 1 ml of water is dropped, natural cheese is cut out and installed, and then the lid of the petri dish is closed.
  • the heat resistant shape retention of natural cheese is 50% or more, preferably 60% or more, more preferably 70% or more, and even more preferably 80% during dry heat heating.
  • the heat resistant shape retention of natural cheese is 40% or more, preferably 50% or more, more preferably 60% or more, and even more preferably 70% during wet heat heating.
  • the heat-resistant shape retention of baking under dry heat assumes that cheese is placed on the surface of bread or the like and baked in an oven or the like. That is, in the present invention, in the measurement (evaluation) of heat-resistant shape retention under dry heat, the temperature for heating the cheese is, for example, 150 to 250 ° C, 170 to 250 ° C, 180 to 250 ° C, 200 to 250 ° C, 200 to 240 ° C. and 210 to 230 ° C.
  • the holding time for heating the cheese is, for example, 5 to 20 minutes, 5 to 18 minutes, 7 to 17 minutes, 8 to 16 minutes, 9 to 15 minutes, 10 to 14 minutes, or 11 to 13 minutes. In addition, these heating temperature and holding time can be set combining each suitably according to the use etc. of cheese.
  • the temperature at which the cheese is heated is, for example, 150 ° C. or higher, preferably 170 ° C. from the viewpoint of effectively evaluating high-temperature heat-resistant shape retention properties.
  • more preferably 180 ° C. or higher further preferably 200 ° C. or higher, more preferably 210 ° C. or higher, and assuming an actual cooking temperature, for example, 250 ° C. or lower, preferably 240 ° C. or lower, more preferably 230 ° C.
  • the lower limit value and the upper limit value can be appropriately set in combination.
  • the holding time for heating the cheese can be appropriately selected and set depending on the temperature for heating the cheese, but the actual cooking temperature is Assuming, for example, 5 minutes or more, preferably 7 minutes or more, more preferably 8 minutes or more, more preferably 9 minutes or more, still more preferably 10 minutes or more, and even more preferably 11 minutes or more. From the viewpoint, for example, 20 minutes or less, preferably 18 minutes or less, more preferably 17 minutes or less, more preferably 16 minutes or less, further preferably 15 minutes or less, further preferably 14 minutes or less, and further preferably 13 minutes or less. Yes, these lower limit value and upper limit value can be appropriately set in combination.
  • the natural cheese of the present invention Since the natural cheese of the present invention has heat-resistant shape retention of baking under dry heat at the temperature and holding time described above, natural cheese is placed on the surface of cooked food such as pizza and gratin. It is suitable for use as a heating material. Therefore, this invention relates also to the cooked food containing the natural cheese of this invention.
  • the heat cooking is not particularly limited as long as the temperature of the object is raised, and includes cooking such as baking, boiling, steaming, boiling, frying, cooking, and frying. Since the natural cheese of the present invention has heat-resistant shape retention after baking under dry heat, it is suitable for use in cooked cooked foods.
  • the heat-resistant shape retention of baking under wet heat assumes that cheese is wrapped in bread or the like and then baked in an oven or the like. That is, in the measurement (evaluation) of heat-resistant shape retention of baking under wet heat, the temperature at which the cheese is heated is, for example, 100 to 200 ° C., 100 to 180 ° C., 100 to 170 ° C., 100 to 160 ° C., 110 to 150 ° C, 120-140 ° C.
  • the holding time for heating the cheese is, for example, 5 to 40 minutes, 10 to 30 minutes, 12 to 28 minutes, 15 to 25 minutes, 16 to 24 minutes, 17 to 23 minutes, or 18 to 22 minutes.
  • these heating temperature and baking time can be set combining each suitably according to the use of cheese.
  • the temperature at which the cheese is heated is, for example, 100 ° C. or higher, preferably from the viewpoint of effectively evaluating high-temperature heat-resistant shape retention properties.
  • the holding time for heating the cheese can be appropriately selected and set depending on the temperature for heating the cheese, but the actual cooking temperature is assumed. Then, for example, 5 minutes or more, preferably 10 minutes or more, more preferably 12 minutes or more, further preferably 15 minutes or more, further preferably 16 minutes or more, further preferably 17 minutes or more, and further preferably 18 minutes or more. From the viewpoint of measuring in a short time, it is, for example, 28 minutes or less, preferably 25 minutes or less, more preferably 24 minutes or less, still more preferably 23 minutes or less, and even more preferably 22 minutes or less.
  • the upper limit value can be set in combination as appropriate.
  • the natural cheese of the present invention Since the natural cheese of the present invention has heat-resistant shape retention under baking at the temperature and holding time described above, for example, cooked food such as bread, manju, gyoza, shumai, spring rolls, etc. It is suitable for use as a material to heat natural cheese wrapped inside. Therefore, the present invention also relates to a cooked food containing the natural cheese of the present invention.
  • the heating cooking is not particularly limited as long as it is a cooking that raises the temperature of the object, and includes cooking such as baking, boiling, steaming, boiling, frying, cooking, and frying. .
  • the natural cheese of the present invention is suitable for use in cooked and cooked foods that are boiled, steamed, and boiled because it has heat-resistant shape retention in baking under wet heat.
  • One embodiment of the present invention has heat-resistant shape retention by baking at 150 to 250 ° C. for 5 to 20 minutes and / or baking at 100 to 200 ° C. for 5 to 40 minutes. It is a method for evaluating natural cheese having heat-resistant shape retention, characterized by having heat-resistant shape retention.
  • the heat-resistant shape retention of 50% or more by baking at 150 to 250 ° C. for 5 to 20 minutes and / or 100 to 200 ° C. for 5 to 40 minutes It is the evaluation method of the natural cheese which has heat-resistant shape retention characterized by having baked under the wet heat of and having 40% or more heat-resistant shape retention property.
  • the present invention is characterized by adding transglutaminase to cheese curd obtained by separating and discharging whey, except for adding transglutaminase, general natural cheese, particularly general ripening
  • a natural cheese manufacturing process can be used, and raw materials other than transglutaminase can be used raw materials used in general natural cheese manufacturing methods.
  • Test Example 1 The raw material milk is prepared so that the fat concentration in the solid content of the product natural cheese is 45% by weight, sterilized by heating at 63 ° C. for 30 minutes, cooled to 33 ° C., and then calcium chloride (0.03 % By weight) was added. Next, 0.1% of a commercial starter for cheese production (manufactured by L. Lactis Danisco) was added to these raw milks. After adding these starters, calf rennet (titer: 15000 units) was added at 0.003% by weight to coagulate the raw milk. The coagulated product was cut and stirred until the whey had a pH of 6.1 to 6.2, and the whey was discharged to obtain curd grains.
  • a commercial starter for cheese production manufactured by L. Lactis Danisco
  • transglutaminase preparation 20% by weight of a transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, stuffed and squeezed, and natural cheese (water content 40.4%) is added. % By weight, milk fat content 45.3% by weight, salt content 1.4% by weight, pH 5.23).
  • transglutaminase preparation KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used. Transglutaminase was added in an amount of 0.002% by weight to the cheese curd (curd grain ratio).
  • Example 1 aged at 4 ° C.
  • Example 2 aged at 7 ° C.
  • Example 3 aged at 12 ° C.
  • Example 4 aged at 20 ° C.
  • Comparative Example 1 aged at 4 ° C.
  • Comparative Example 2 aged at 12 ° C.
  • Heat resistant shape retention is calculated from the height of the sample before and after heating.
  • the heat-resistant shape retention was calculated as follows, and when the heat-resistant shape retention during dry heat heating was 50% or more, it was evaluated that there was heat-resistant shape retention.
  • Heat-resistant shape retention (%) height of cheese after heating / height of cheese before heating ⁇ 100
  • Heat resistant shape retention is calculated from the height of the sample before and after heating.
  • the heat-resistant shape retention was calculated as follows, and when the heat-resistant shape retention during wet heat heating was 40% or more, it was evaluated that there was heat-resistant shape retention.
  • Heat-resistant shape retention (%) height of cheese after heating / height of cheese before heating ⁇ 100
  • transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, packed and squeezed, and natural cheese (moisture 39.2) is added. % By weight, 47.1% by weight of milk fat, 1.45% by weight of salt, pH 5.39).
  • transglutaminase preparation KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used. Transglutaminase was added in an amount of 0.002% by weight as a curd grain ratio.
  • Example 5 aged at 12 ° C.
  • Comparative Example 3 aged at 12 ° C.
  • transglutaminase preparation 20% by weight of a transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, packed and squeezed, and natural cheese (water content 41.9) is added. % By weight, milk fat content 51.7% by weight, salt content 1.3% by weight, pH 5.27).
  • transglutaminase preparation KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used. Transglutaminase was added in an amount of 0.002% by weight as a curd grain ratio.
  • Example 6 aged at 12 ° C.
  • Comparative Example 4 aged at 12 ° C.
  • transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, packed and squeezed, and natural cheese (moisture 48.8) is added. % By weight, milk fat content 2.81% by weight, salt content 1.33% by weight, pH 5.28).
  • transglutaminase preparation KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used.
  • Transglutaminase was added in an amount of 0.002% by weight as a curd grain ratio.
  • a natural cheese to which no transglutaminase was added was produced in the same manner as described above. These natural cheeses were ripened at 12 ° C., and the heat-retaining shape was measured in the same manner as in Test Example 1 for 1 month, 2 months, 3 months, and 4 months.
  • the system to which transglutaminase was added was referred to as Example 7 (12 ° C. aging), and the system to which no transglutaminase was added was referred to as Comparative Example 5 (12 ° C. aging).
  • Example 7 As shown in Tables 7 to 8, as a result of measuring the heat retaining shape after aging, Example 7 to which 0.002% of transglutaminase was added had heat retaining shape after 1 month of aging. On the other hand, Comparative Example 5 in which no transglutaminase was added did not have heat-resistant shape retention as compared with Example 7.
  • Test Example 5 The raw milk is prepared so that the fat concentration in the solid content of the product natural cheese is 40% by weight, sterilized by heating at 63 ° C. for 30 minutes, cooled to 33 ° C., and then calcium chloride (0.03 % By weight) was added. Next, a commercial starter for cheese manufacture was added to these raw milks. After adding these starters, calf rennet (titer: 15000 units) was added at 0.003% by weight to coagulate the raw milk. The coagulated product was cut and stirred until the whey had a pH of 6.1 to 6.2, and the whey was discharged to obtain curd grains.
  • transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, stuffed and squeezed, and natural cheese (water content 45.5%) is added. % By weight, milk fat content 40.0% by weight, salt content 1.43% by weight, pH 5.30).
  • transglutaminase preparation KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used. Transglutaminase was added in an amount of 0.002% by weight as a curd grain ratio.
  • Example 8 (12 ° C. aging
  • Comparative Example 6 (12 ° C. aging
  • Comparative Example 7 Addition of transglutaminase during curdling of raw milk (before whey discharge) (lactic acid fermentation process) After sterilizing and cooling 11 kg of raw milk (fat content 3.40%), the mixture was heated to 31 ° C., and 2.25 g of mixed lactic acid bacteria starter (manufactured by L. lactis and L. Cremoris, Chris. Hansen's Laboratories) was added. Hold at 31 ° C. for minutes. (Rennetting process) When the lactic acid fermentation process had passed for 35 minutes, 0.72 ml of Anato dye was added, and after 5 minutes, 0.02% of calcium chloride was added.
  • calf rennet single strength, manufactured by Chris. Hansen's Laboratories, strength 14000 units
  • the amount of transglutaminase added was 10 U per 1 g of protein in milk as a raw material (denoted as 10 U / gp).
  • the temperature was raised from 33 ° C. to 35 ° C. over 15 minutes, and further heated from 35 ° C. to 38 ° C. for 10 minutes. Throughout the cooking process, stirring was always continued slowly so as not to break the curd grains.
  • Comparative Example 8 Addition of transglutaminase before curding raw material milk (rennetting process) Instead of adding transglutaminase during curdling raw material milk (before whey discharge), adding transglutaminase is the addition of rennet. Cheddar cheese was manufactured in the same process as in Comparative Example 7 except that it was performed simultaneously.
  • Example 9 Addition of transglutaminase after curding raw milk (milling and salt addition step) Crushed cheese after discharging whey instead of adding transglutaminase during curding raw material (before discharging whey) Cheddar cheese was produced in the same process as Comparative Example 7 except that transglutaminase was added to the curd. The amount of transglutaminase added was 0.02% by weight in terms of curd grain ratio.
  • Example 9 in which transglutaminase was added to the cheese curd after curdling and whey discharge had heat-resistant shape retention after 1 month of aging. It was.
  • Comparative Example 7 in which transglutaminase was added to the curd and Comparative Example 8 in which transglutaminase was added simultaneously with the addition of rennet did not have heat-resistant shape retention.
  • water separation occurred during the aging process.
  • Table 13 shows the visual evaluation and the evaluation of the ripening flavor of the natural cheeses of Comparative Example 7, Comparative Example 8, and Example 9 at each time point of the ripening period of 1 month to 6 months.
  • Comparative Example 7 and Comparative Example 8 water separation occurred during the aging process.
  • ripening flavor ripening at 12 ° C. for 3 months or more resulted in a natural cheese having a strong ripening flavor.
  • the present inventors have found a natural cheese having heat-resistant shape-retaining properties and a method for producing the same without adjusting the composition of the moisture and milk protein of the raw milk.
  • This natural cheese can provide a novel natural cheese having a good heat-resistant shape retaining property and a method for producing the same without causing the cheese to melt or deform when cooking or processing such as heating in a frying pan or an oven.

Abstract

 Provided are a natural cheese exhibiting heat-resistant shape retention without loss of the latent natural-cheese composition, and a method for manufacturing said cheese. It was found that adding transglutaminase to a cheese curd of a natural cheese obtained after curdling raw material milk and discharging the whey, and maturing the resulting cheese curd, makes it possible to manufacture a natural cheese exhibiting heat-resistant shape retention. It was found that in this case, a natural cheese exhibiting heat-resistant shape retention can be manufactured without increasing the composition of water and milk protein in the raw material milk. Specifically, it is possible to provide: a novel natural cheese which exhibits excellent heat-resistant shape retention and neither melts nor deforms when cooked or processed such as by heating in a frying pan or an oven; and a method for manufacturing the natural cheese.

Description

耐熱保形性を有するナチュラルチーズおよびその製造方法Natural cheese having heat-retaining shape and method for producing the same
 本発明は、耐熱保形性を有するナチュラルチーズおよびその製造方法に関する。 The present invention relates to natural cheese having heat-resistant shape retention and a method for producing the same.
 日本の市場において、ナチュラルチーズが定着しつつある。ナチュラルチーズは、熟成の程度により、乳成分の熟成の風味を楽しめる、いわゆる熟成型ナチュラルチーズ、および新鮮な乳風味を味わえる、いわゆる非熟成型ナチュラルチーズに分類することができる。また、ナチュラルチーズは、その硬さから、特別硬質ナチュラルチーズ、硬質ナチュラルチーズ、半硬質ナチュラルチーズ、軟質ナチュラルチーズに分類することができる。このように、ナチュラルチーズには、熟成の程度(有無)や物性(食感)の違いなどにより、多くの種類が存在する。 Natural cheese is becoming established in the Japanese market. Natural cheese can be classified according to the degree of ripening into so-called mature natural cheese that can enjoy the ripening flavor of milk components, and so-called non-ripened natural cheese that can taste a fresh milk flavor. Natural cheese can be classified into special hard natural cheese, hard natural cheese, semi-hard natural cheese, and soft natural cheese based on its hardness. Thus, there are many types of natural cheese depending on the degree of ripening (presence / absence) and the physical properties (texture).
 ナチュラルチーズは、その食シーンとして、そのまま食べる場合もあるが、チーズの特性を利用して調理・加工された状態で食べる場合もある。ここで、例えば、ピザ、グラタン、ラザニアなどのような場合には、チーズが加熱により溶融し、糸曳き性を増す特性が積極的に利用される。一方、製菓・製パン用、フライ・天ぷらなどの揚げもの、炒めもの、焼きものの具材などのような場合には、チーズが加熱により溶融し、糸曳き性が増す特性は求められておらず、チーズが加熱により溶融しにくく、ある程度で元の形状をそのまま維持できる耐熱保形性が求められる。なお、これらの耐熱保形性を増したチーズには、プロセスチーズやチーズフードの形態が多い。 Natural cheese may be eaten as it is as a food scene, but it may be eaten as it is cooked and processed using the characteristics of cheese. Here, for example, in the case of pizza, gratin, lasagna, etc., the characteristic that the cheese melts by heating and increases the stringiness is actively utilized. On the other hand, in the case of confectionery / baking, fried foods such as frying and tempura, fried foods, ingredients for baked goods, etc., the characteristics that the cheese melts by heating and increases the stringiness are not required. The cheese is not easily melted by heating, and heat-resistant shape retention that can maintain the original shape to some extent is required. In addition, there are many forms of process cheese and cheese food in the cheese which increased these heat-resistant shape retention properties.
 特許文献1には、ナチュラルチーズまたはプロセスチーズを主原料とし、これと水および溶融塩並びに所望により各種添加物、例えば、牛乳由来の蛋白質、調味料および香料、とを用いて融解調製したチーズ溶融物に、トランスグルタミナーゼを作用させることを特徴とする、加熱による融解変形性(易融性)および曳糸性を適度に併有する、または加熱による融解変形の生じない(耐熱保型性)、歯切れの良好なカマボコ様の組織を有するチーズフードの製造法が記載されている。 Patent Document 1 discloses a cheese melt prepared by melting natural cheese or processed cheese as a main raw material and using water, molten salt, and optionally various additives such as milk-derived proteins, seasonings and flavors. It is characterized by having transglutaminase act on the product, having moderately both melting deformation (easy melting) and spinnability by heating, or no melting deformation by heating (heat-resistant shape retention), crisp A method for producing a cheese food having a good crumb-like structure is described.
 ナチュラルチーズは一般的に、自然な乳風味と独自の組織、および物性を有しており、このようなナチュラルチーズの組織、および物性を好む消費者にとって、プロセスチーズやチーズフードでは物足りないことがある。耐熱性を有するナチュラルチーズでは、例えば、キプロスのハロウミが知られている。ハロウミは、耐熱保形性を得るために、乳酸菌を使用せずに、中性のpHのまま、原料乳を約90℃の高温のホエイ中で加熱して製造する。 Natural cheese generally has a natural milk flavor and unique structure and properties, and consumers who prefer such natural cheese structure and properties may not be satisfied with processed cheese or cheese food. . In natural cheese having heat resistance, for example, Cyprus harrows are known. In order to obtain heat-resistant shape retention, harrowmi is produced by heating raw material milk in a high-temperature whey at about 90 ° C. while maintaining a neutral pH without using lactic acid bacteria.
 特許文献2には、乳タンパク質濃縮物粉末を水、生乳、または脱脂乳で溶解し、乳脂肪を添加後、均質処理を行って、原料乳を調製し、この原料乳に、乳酸菌スターターまたは酸性化剤、および凝乳酵素を添加してカードを形成させて得ることができる、耐熱性および耐水性を有することを特徴とするナチュラルチーズが記載されている。 In Patent Document 2, milk protein concentrate powder is dissolved in water, raw milk, or skim milk, and after adding milk fat, homogeneous treatment is performed to prepare raw milk, and this raw milk is mixed with lactic acid bacteria starter or acidic A natural cheese characterized by having heat resistance and water resistance, which can be obtained by adding curatives and curdling enzymes to form curds is described.
特開平2-131537号公報Japanese Patent Laid-Open No. 2-131537 特開2002-95410号公報JP 2002-95410 A 特開平8-173032号公報JP-A-8-173032 特開2002-065158号公報Japanese Patent Laid-Open No. 2002-065158 特開2003-134997号公報JP 2003-134997 A
 特許文献1のチーズフードは、本来のナチュラルチーズの有する自然な乳風味と独自の組織、および物性を欠くものとなっている。また、前記の耐熱保形性の有するナチュラルチーズであるハロウミの製造工程において、通常のナチュラルチーズの製造装置の他に、専用の加熱装置が必須である。そのため、その製造工程が煩雑となり、新たに設備投資する必要があることなどから、商業的な大量生産として実用化することは容易ではない。また、ハロウミでは、乳酸菌による発酵工程などが存在せず、原料乳のpHが中性のままであるため、衛生的な観点などを勘案し、全体の塩分が3~4重量%になるまで加塩する必要がある。そのため、その塩分が高くなり、塩分の過剰摂取による健康への影響が懸念される。さらに、ハロウミの製造工程では、乳酸菌による熟成工程などが存在せず、いわゆる非熟成型のナチュラルチーズであるため、乳成分の熟成の風味を楽しめない。 The cheese food of Patent Document 1 lacks the natural milk flavor and original structure and physical properties of the original natural cheese. Moreover, in the manufacturing process of the harrow which is the natural cheese which has the said heat-resistant shape retention property, a dedicated heating apparatus is essential in addition to the normal natural cheese manufacturing apparatus. For this reason, the manufacturing process becomes complicated, and it is necessary to newly invest in facilities, so that it is not easy to put it to practical use as commercial mass production. In addition, hallowmi does not have a fermentation process with lactic acid bacteria, and the pH of the raw milk remains neutral. Therefore, in view of hygiene, etc., salt is added until the total salt content is 3 to 4% by weight. There is a need to. Therefore, the salt content becomes high, and there is a concern about the health effect of excessive intake of salt. Furthermore, in the production process of harrow, there is no aging process with lactic acid bacteria, and so-called non-ripe-molded natural cheese, so that the flavor of aging of milk components cannot be enjoyed.
 特許文献2の耐熱保形性を有するナチュラルチーズでは、乳タンパク質濃縮物(MPC)を溶解して、乳脂肪を添加するなどしており、本来のナチュラルチーズに比べて、高タンパク質、低乳糖などとなっている。そのため、牛乳を原料として製造したナチュラルチーズの組成とは大きく異なったものとなり、本来のナチュラルチーズの有する自然な乳風味や独自の組織、および物性を欠くものとなっている。 In the natural cheese having heat-resistant shape retention of Patent Document 2, the milk protein concentrate (MPC) is dissolved and milk fat is added. Compared with the original natural cheese, high protein, low lactose, etc. It has become. For this reason, the composition of natural cheese produced using milk as a raw material is greatly different, and the natural milk flavor, original structure, and physical properties of natural cheese are lacking.
 特許文献3~5には、ナチュラルチーズを調製するにあたり、その原料乳を凝乳させる前、または原料乳を凝乳させると同時に、トランスグルタミナーゼを添加して、ホエイの分離を抑制させる方法が記載されている。ただし、本発明者らによれば、この方法では、従来と品質が異なるホエイが発生することとなるため、ホエイの処理が煩雑になり、また、熟成中に離水が生じたり、食感が悪くなるなどの問題が見出されている。 Patent Documents 3 to 5 describe a method for suppressing separation of whey by adding transglutaminase before curling the raw milk or simultaneously with curling the raw milk in preparing natural cheese. Has been. However, according to the present inventors, this method generates whey having a quality different from that of the prior art, which makes the whey treatment complicated, causes water separation during aging, and has a poor texture. Problems such as becoming are found.
 このような状況に対し、本発明は、ナチュラルチーズの本来の風味や組成を損なうことなく、耐熱保形性を有するナチュラルチーズおよびその製造方法を提供することが課題である。 In such a situation, the present invention has an object to provide natural cheese having heat-resistant shape retention and a method for producing the same without impairing the original flavor and composition of natural cheese.
 本発明者らは、前記の課題を解決すべく、鋭意研究を重ねた結果、原料乳を凝乳させた後に、ホエイを排出した後のナチュラルチーズのチーズカードにトランスグルタミナーゼを添加(配合)して熟成させることにより、耐熱保形性を有するナチュラルチーズを製造できることを見出し、本発明を完成させた。 As a result of intensive research to solve the above-mentioned problems, the present inventors added (mixed) transglutaminase to the cheese curd of natural cheese after discharging the whey after curling the raw milk. As a result, it was found that natural cheese having heat-resistant shape retention can be produced by aging, and the present invention was completed.
 すなわち、本発明の耐熱保形性を有するナチュラルチーズおよびその製造方法において、原料乳に水分および乳タンパク質の組成を調整することなく、耐熱保形性を有するナチュラルチーズを実現することができる。また、本発明の製造方法において、ホエイを排出するまでの工程は、通常(従来)の製造方法と同様の工程を採用することができ、本発明の原料乳の凝乳により発生するホエイは、通常のナチュラルチーズの製造方法により発生するホエイと同等の物性や品質(同量、同組成など)であるため、ホエイを再利用する工程が煩雑になる問題なども生じない。また、本発明の製造方法において、従来と品質が同じ通常のホエイが発生することとなるため、熟成中に離水が生じたり、食感が悪くなるなどの問題が起こらない。 That is, in the natural cheese having heat-resistant shape retention and the method for producing the same of the present invention, natural cheese having heat-resistant shape-retaining property can be realized without adjusting the composition of moisture and milk protein in the raw milk. Further, in the production method of the present invention, the process until discharging whey can adopt the same process as the normal (conventional) production method, and whey generated by curding raw material milk of the present invention is: Since it has the same physical properties and quality (same amount, same composition, etc.) as whey generated by the production method of ordinary natural cheese, there is no problem that the process of reusing whey is complicated. Further, in the production method of the present invention, normal whey having the same quality as the conventional one is generated, so that problems such as water separation during aging and poor texture do not occur.
 したがって、本発明は、以下に関する。
(1) 原料乳を凝乳させた後にホエイを排出してチーズカードを調製し、当該チーズカードにトランスグルタミナーゼを添加して熟成させることを含む、耐熱保形性を有するナチュラルチーズの製造方法。
(2) 凝乳酵素と乳酸菌を添加して凝乳させる、前記(1)に記載の製造方法。
(3) 熟成させることが、4~20℃、1~4ヶ月間で熟成させることである、前記(1)または(2)に記載の製造方法。
(4) トランスグルタミナーゼを添加した後に、トランスグルタミナーゼが失活する温度および時間で加熱することを含まない、前記(1)~(3)のいずれかに記載の製造方法。
(5) トランスグルタミナーゼを添加した後かつ熟成する前に、トランスグルタミナーゼが失活する温度および時間で加熱することを含まない、前記(1)~(3)のいずれかに記載の製造方法。
Accordingly, the present invention relates to the following.
(1) A method for producing natural cheese having heat-resisting shape-retaining properties, comprising curling raw material milk, preparing whey and preparing cheese curd, adding transglutaminase to the cheese curd and aging.
(2) The production method according to (1), wherein a milk-clotting enzyme and a lactic acid bacterium are added to cause the milk to coagulate.
(3) The production method according to the above (1) or (2), wherein the aging is aging at 4 to 20 ° C. for 1 to 4 months.
(4) The production method according to any one of (1) to (3) above, which does not include heating at a temperature and a time at which transglutaminase is deactivated after adding transglutaminase.
(5) The production method according to any one of (1) to (3) above, which does not include heating at a temperature and a time at which transglutaminase is inactivated after adding transglutaminase and before aging.
(6) 前記(1)~(5)のいずれかに記載の製造方法により製造された、耐熱保形性を有するナチュラルチーズ。
(7) 150~250℃、5~20分間の乾熱加熱および/または100~200℃、5~40分間の湿熱加熱において保形性を有する、前記(6)に記載のナチュラルチーズ。
(8) 150~250℃、5~20分間の乾熱加熱において50%以上の保形性、および/または100~200℃、5~40分間の湿熱加熱において40%以上の保形性を有する、前記(6)または(7)に記載のナチュラルチーズ。
(9) 前記(6)~(8)のいずれかに記載のナチュラルチーズを含む、加熱調理食品。
(10) 前記(6)~(8)のいずれかに記載のナチュラルチーズを内包して含む、加熱調理食品。
(11) 加熱調理食品用である、前記(6)~(8)のいずれかに記載のナチュラルチーズ。
(6) Natural cheese having heat-resistant shape retention produced by the production method according to any one of (1) to (5).
(7) The natural cheese according to (6), which has shape-retaining properties at 150 to 250 ° C., 5 to 20 minutes of dry heat heating and / or 100 to 200 ° C. and 5 to 40 minutes of wet heat heating.
(8) It has a shape retention of 50% or more in dry heat heating at 150 to 250 ° C. for 5 to 20 minutes and / or a shape retention of 40% or more in wet heat heating at 100 to 200 ° C. for 5 to 40 minutes. The natural cheese according to (6) or (7).
(9) A cooked food containing the natural cheese according to any one of (6) to (8).
(10) A cooked food containing the natural cheese according to any one of (6) to (8).
(11) The natural cheese according to any one of (6) to (8), which is for cooked food.
 さらに、本発明は、次の通りとなる。
[1]原料乳を凝乳させた後にホエイを排出してチーズカードを調製し、当該チーズカードにトランスグルタミナーゼを添加し、熟成させることを特徴とする、耐熱保形性を有するナチュラルチーズの製造方法。
[2]凝乳酵素と乳酸菌を添加して凝乳させることを特徴とする、前記[1]に記載の耐熱保形性を有するナチュラルチーズの製造方法、
[3]ナチュラルチーズが熟成型ナチュラルチーズであることを特徴とする、前記[1]または[2]に記載の耐熱保形性を有するナチュラルチーズの製造方法、
[4]ナチュラルチーズが、150~250℃にて5~20分間の乾熱下での焼成を行い、保形性を有すること、および/または100~200℃にて5~40分間の湿熱下での焼成を行い、保形性を有することを特徴とする、前記[1]~[3]に記載の耐熱保形性を有するナチュラルチーズの製造方法、
Furthermore, the present invention is as follows.
[1] Manufacture of natural cheese having heat-resistant shape retention, characterized in that after curding raw milk, whey is discharged to prepare a cheese curd, and transglutaminase is added to the cheese curd and ripened. Method.
[2] A method for producing natural cheese having heat-resistant shape-holding properties according to the above [1], wherein a milk-clotting enzyme and lactic acid bacteria are added and allowed to curd up.
[3] The method for producing natural cheese having heat-resistant shape-holding properties according to [1] or [2], wherein the natural cheese is a mature molded natural cheese,
[4] Natural cheese is baked under dry heat for 5 to 20 minutes at 150 to 250 ° C. and has shape retention, and / or under wet heat for 5 to 40 minutes at 100 to 200 ° C. The method for producing natural cheese having heat-resistant shape-retaining property according to any one of [1] to [3] above, characterized in that it has a shape-retaining property.
[5]ナチュラルチーズが、150~250℃にて5~20分間の乾熱下での焼成を行い、50%以上の保形性を有すること、および/または100~200℃にて5~40分間の湿熱下での焼成を行い、40%以上の保形性を有することを特徴とする、前記[1]~[3]に記載の耐熱保形性を有するナチュラルチーズの製造方法、
[6]熟成が4~20℃で1~4ヶ月間の条件であることを特徴とする、前記[1]~[5]に記載の耐熱保形性を有するナチュラルチーズの製造方法、
[7]前記[1]~[6]に記載の製造方法で製造された耐熱保形性を有するナチュラルチーズ。
[5] Natural cheese is baked under dry heat for 5 to 20 minutes at 150 to 250 ° C. and has a shape retention of 50% or more, and / or 5 to 40 at 100 to 200 ° C. The method for producing natural cheese having heat-resistant shape-retaining properties according to the above [1] to [3], characterized by performing baking under wet heat for minutes and having shape-retaining properties of 40% or more,
[6] The method for producing natural cheese having heat-resistant shape-holding properties according to the above [1] to [5], wherein ripening is performed at 4 to 20 ° C. for 1 to 4 months,
[7] A natural cheese having heat-resistant shape retention produced by the production method according to the above [1] to [6].
 本発明では、原料乳を凝乳させた後にホエイを排出してチーズカードを調製し、当該チーズカードにトランスグルタミナーゼを添加して熟成させることを特徴とし、フライパンやオーブンの加熱などで調理・加工したときに、溶融や変形することなく、耐熱保形性が良好な新規のナチュラルチーズおよびその製造方法を提供できる。 In the present invention, after curding raw milk, whey is discharged to prepare a cheese curd, and transglutaminase is added to the cheese curd and matured, and cooking and processing by heating in a frying pan or oven, etc. When this is done, it is possible to provide a novel natural cheese having good heat-resistant shape retention and a method for producing the same without melting or deformation.
 以下では、本発明を詳細に説明するが、本発明は、個々の形態には限定されない。
 本発明において「ナチュラルチーズ」とは、乳、バターミルク、脱脂乳、クリームまたはこれらを混合したもののほとんど全部、もしくは一部のタンパク質を酵素その他の凝固剤により凝固させた凝乳から、ホエイの一部を除去したもの、またはこれらを熟成したものをいう。
 本発明において「原料乳」とは、原料として用いられる乳を意味し、典型的には、未殺菌の生乳のことをいい、牛乳、羊乳、水牛乳、山羊乳などの獣乳を用いることができる。そして、本発明において、実際に目的とするナチュラルチーズに応じて、原料乳の組成を調整することができる。例えば、クリームセパレーターなどを用いて、未殺菌の生乳から脱脂乳およびクリームを分離し、これら脱脂乳およびクリームを様々な混合比率で配合して、乳脂肪の含量を調整することができる。また、公知の分離膜などを用いて、ミネラル、ビタミン、乳糖、乳タンパク質、乳脂肪などを分離し、これらの成分を混合比率で配合して、これらの成分の含量を調整することもできる。また、生乳に、バターミルク、脱脂乳、クリームなどを混合し、乳脂肪の含量、無脂乳固形分の含量を調整することもできる。
Hereinafter, the present invention will be described in detail, but the present invention is not limited to individual forms.
In the present invention, “natural cheese” refers to milk, buttermilk, skimmed milk, cream or a mixture of these, or from a coagulated milk obtained by coagulating a part of protein with an enzyme or other coagulant. This is the product from which the part has been removed or the product that has been aged.
In the present invention, “raw milk” means milk used as a raw material, and typically refers to raw milk that has not been sterilized, and uses animal milk such as cow's milk, sheep milk, buffalo milk, and goat milk. Can do. And in this invention, the composition of raw material milk can be adjusted according to the natural cheese actually made into the objective. For example, skim milk and cream can be separated from raw milk that has not been sterilized using a cream separator or the like, and the skim milk and cream can be blended in various mixing ratios to adjust the content of milk fat. In addition, minerals, vitamins, lactose, milk protein, milk fat, and the like can be separated using a known separation membrane and the like, and these components can be blended in a mixing ratio to adjust the content of these components. Moreover, butter milk, skim milk, cream, etc. can be mixed with raw milk, and the content of milk fat and the content of non-fat milk solids can also be adjusted.
 本発明の一態様において、前記の通り、原料乳の乳脂肪の含量を調整することができる。原料乳の乳脂肪分の含量は、原料乳の全固形分中の乳脂肪分の割合で表すことができる。例えば、原料乳の全固形分中の乳脂肪分の割合が低ければ、いわゆる低脂肪タイプのナチュラルチーズとなり、原料乳の全固形分中の乳脂肪分の割合が高ければ、いわゆる高脂肪タイプのナチュラルチーズとなる。原料乳の全固形分中の乳脂肪分の割合は、特に制限されないが、例えば、0~80重量%、0~70重量%、0.5~65重量%、0.5~60重量%、1~55重量%、2~55重量%、5~55重量%、10~55重量%、15~55重量%、20~55重量%、25~55重量%、30~55重量%、35~55重量%である。 In one embodiment of the present invention, as described above, the content of milk fat in raw milk can be adjusted. The milk fat content of the raw milk can be expressed as the ratio of the milk fat content in the total solid content of the raw milk. For example, if the proportion of milk fat in the total solid content of raw material milk is low, it becomes a so-called low fat type natural cheese, and if the proportion of milk fat in the total solid content of raw material milk is high, it is a so-called high fat type. Natural cheese. The ratio of the milk fat content in the total solid content of the raw material milk is not particularly limited, but for example, 0 to 80% by weight, 0 to 70% by weight, 0.5 to 65% by weight, 0.5 to 60% by weight, 1-55 wt%, 2-55 wt%, 5-55 wt%, 10-55 wt%, 15-55 wt%, 20-55 wt%, 25-55 wt%, 30-55 wt%, 35- 55% by weight.
 本発明の一態様において、無脂肪タイプのナチュラルチーズの場合には、原料乳の全固形分中の乳脂肪分の割合は0重量%、低脂肪タイプのナチュラルチーズの場合には、原料乳の全固形分中の乳脂肪分の割合の下限値は0.5重量%、好ましくは1重量%、より好ましくは2重量%、さらに好ましくは5重量%であり、上限値は20重量%、好ましくは15重量%、より好ましくは10重量%、さらに好ましくは5重量%である。このとき、原料乳の全固形分中の乳脂肪分の割合の下限値と上限値とを適宜組み合わせて設定することができる。本発明の一態様において、通常の脂肪含量のナチュラルチーズの場合には、原料乳の全固形分中の乳脂肪分の割合の下限値は20重量%、好ましくは25重量%、より好ましくは30重量%、さらに好ましくは35重量%であり、上限値は80重量%、好ましくは70重量%、より好ましくは65重量%、さらに好ましくは60重量%、さらにより好ましくは55重量%である。このとき、原料乳の全固形分中の乳脂肪分の割合の下限値と上限値とを適宜組み合わせて設定することができる。 In one embodiment of the present invention, in the case of a non-fat type natural cheese, the ratio of the milk fat content in the total solid content of the raw material milk is 0% by weight. The lower limit of the ratio of milk fat content in the total solid content is 0.5% by weight, preferably 1% by weight, more preferably 2% by weight, still more preferably 5% by weight, and the upper limit is 20% by weight, preferably Is 15% by weight, more preferably 10% by weight, still more preferably 5% by weight. At this time, the lower limit value and the upper limit value of the ratio of the milk fat content in the total solid content of the raw material milk can be set in appropriate combination. In one aspect of the present invention, in the case of natural fat-containing natural cheese, the lower limit of the ratio of milk fat content in the total solid content of raw milk is 20% by weight, preferably 25% by weight, more preferably 30%. The upper limit is 80% by weight, preferably 70% by weight, more preferably 65% by weight, still more preferably 60% by weight, and still more preferably 55% by weight. At this time, the lower limit value and the upper limit value of the ratio of the milk fat content in the total solid content of the raw material milk can be set in appropriate combination.
 本発明の一態様において、一般的に知られた原料乳の殺菌方法により、原料乳を殺菌処理してもよい。このとき、一般的に知られたナチュラルチーズの製造方法で用いられる低温殺菌方法により、原料乳を殺菌処理すると、殺菌処理する前の原料乳に存在するリパーゼを失活させながら、殺菌処理する前の原料乳に存在する一般細菌などの生菌数を低減できて、品質の良好なナチュラルチーズを製造する上で好ましい。 In one embodiment of the present invention, raw material milk may be sterilized by a generally known raw material sterilization method. At this time, when the raw milk is sterilized by a pasteurization method used in a generally known natural cheese manufacturing method, the lipase present in the raw milk before sterilization is deactivated, and before sterilization The number of viable bacteria such as general bacteria present in the raw milk can be reduced, and this is preferable in producing natural cheese with good quality.
 本発明において「凝乳」とは、原料乳を固めることをいい、一般的に知られたチーズカードを形成させる作用を包含する。このとき、本発明において、原料乳を凝乳する方法では、公知の方法を使用することができる。例えば、原料乳にレンネットなどの凝乳酵素を添加(配合)する方法、原料乳のpHを調整してから加熱する方法、原料乳に乳酸菌を添加し、乳酸菌により発酵して、原料乳のpHを低下させ、原料乳を凝集させる方法などを使用することができる。そして、本発明において、原料乳にレンネットなどの凝乳酵素を添加(配合)する方法、原料乳のpHを調整してから加熱する方法、原料乳に乳酸菌を添加し、乳酸菌により発酵して、原料乳のpHを低下させ、原料乳を凝集させる方法では、これらを単独で使用してもよいし、これらを併用してもよい。ここで、乳酸菌は、ナチュラルチーズで使用することのできる乳酸菌であればよく、その属および種は任意であり、例えば、ラクチス菌、クレモリス菌、ブルガリア菌、サーモフィラス菌などの公知の乳酸菌を挙げることができる。 In the present invention, “curd milk” means to harden raw material milk and includes an action of forming a generally known cheese curd. At this time, in this invention, a well-known method can be used in the method of coagulating raw material milk. For example, a method of adding (compounding) a curdling enzyme such as rennet to raw milk, a method of heating after adjusting the pH of raw milk, adding lactic acid bacteria to raw milk, fermenting with lactic acid bacteria, A method of lowering the pH and aggregating the raw milk can be used. In the present invention, a method of adding (compounding) a curdling enzyme such as rennet to raw milk, a method of heating after adjusting the pH of raw milk, adding lactic acid bacteria to raw milk and fermenting with lactic acid bacteria In the method of lowering the pH of raw material milk and aggregating raw material milk, these may be used alone or in combination. Here, the lactic acid bacterium may be any lactic acid bacterium that can be used in natural cheese, and its genus and species are arbitrary, and examples thereof include known lactic acid bacteria such as Lactis, Cremoris, Bulgaria, and Thermophilus. Can do.
 本発明において「チーズカード」とは、例えば、原料乳の凝乳により分離したホエイの全部または一部を排出して得られるものをいう。このとき、本発明において、ホエイを排出する方法として、一般的に知られたナチュラルチーズの製造方法で用いられる方法を用いることができる。また、ホエイを排出する程度では、その後の熟成工程に耐えられる程度であれば、特に制限されないが、一般的に知られたナチュラルチーズの製造方法で用いられる程度と同量に設定することができる。本発明において「ホエイ」とは、例えば、原料乳の凝乳により生成した凝固物を分離して得られるものをいい、ホエイタンパク質などを含む水分をいう。本発明において「ホエイを排出する」とは、例えば、原料乳の凝乳により生成した凝固物とホエイが共存している状態から、ホエイを排出させることをいう。 In the present invention, “cheese curd” refers to a product obtained by discharging all or part of whey separated by curdling raw material milk. At this time, in the present invention, as a method for discharging whey, a method used in a generally known method for producing natural cheese can be used. Moreover, as long as the whey is discharged, it is not particularly limited as long as it can withstand the subsequent ripening step, but it can be set to the same amount as that used in a generally known method for producing natural cheese. . In the present invention, “whey” refers to, for example, a product obtained by separating a coagulated product produced by curdling raw material milk, and refers to water containing whey protein and the like. In the present invention, “to discharge whey” means, for example, to discharge whey from a state where coagulated material and whey produced by curdling raw material milk coexist.
 本発明では、原料乳を凝乳させた後に、ホエイを排出させたチーズカードに、トランスグルタミナーゼを添加することを特徴とする。そのため、この排出されたホエイでは、本発明以外の一般的に知られたナチュラルチーズの製造により生成されるホエイと、その生成量および組成が同じであり、その後に、従来と同様にして、効率良くホエイを加工することができる。ここで、ホエイを加工する方法では、公知の方法を使用することができる。例えば、ホエイパウダー、脱塩ホエイパウダー、ホエイタンパク質濃縮物(WPC)、ホエイタンパク質単離物(WPI)、パーミエイト、脱乳糖パーミエイト、乳糖へ加工する方法を使用することができる。 The present invention is characterized in that transglutaminase is added to cheese curd from which whey has been discharged after the raw milk has been coagulated. Therefore, in this discharged whey, the amount and composition of whey produced by the production of generally known natural cheese other than the present invention are the same. Whey can be processed well. Here, as a method for processing whey, a known method can be used. For example, a method of processing into whey powder, desalted whey powder, whey protein concentrate (WPC), whey protein isolate (WPI), permeate, delactose permeate, or lactose can be used.
 トランスグルタミナーゼは一般的に、タンパク質またはペプチド鎖内のグルタミン残基のγ-カルボキシアミド基と一級アミンとのアシル転移反応を触媒し、一級アミンがタンパク質のリジン残基である場合には、ε-(γ-Glu)-Lys架橋結合を形成させる作用があることが知られている。 Transglutaminase generally catalyzes an acyl transfer reaction between a γ-carboxamide group of a glutamine residue in a protein or peptide chain and a primary amine, and if the primary amine is a lysine residue of the protein, ε- It is known to have an effect of forming (γ-Glu) -Lys crosslinks.
 トランスグルタミナーゼを添加し、トランスグルタミナーゼを作用させたナチュラルチーズでは、これまでに、原料乳にトランスグルタミナーゼを添加し、ホエイの分離および排出を抑制して、原料乳を基準とした収率を向上することが知られていた。これに対して、本発明では、あえてホエイを分離および排出し、このホエイを排出した後のチーズカードにトランスグルタミナーゼを添加して、その後の熟成工程においてトランスグルタミナーゼを作用させることにより、従来のナチュラルチーズの組成(例えば、水分量)や品質などを維持しながら、ナチュラルチーズの耐熱保形性を向上することができた。 In natural cheese with transglutaminase added and allowed to act on transglutaminase, transglutaminase has been added to raw milk so far to improve the yield based on raw milk by suppressing the separation and discharge of whey. It was known. In contrast, in the present invention, whey is intentionally separated and discharged, transglutaminase is added to the cheese curd after the whey is discharged, and transglutaminase is allowed to act in the subsequent ripening step, thereby allowing the conventional natural While maintaining the composition (for example, water content) and quality of cheese, the heat resistant shape retention of natural cheese could be improved.
 一般的なナチュラルチーズの製造方法は、
(1)原料乳を凝集して、凝固物およびホエイを生成する工程、
(2)凝固物を切断し、凝固物(の内部)からホエイを分離する工程、
(3)ホエイを排出する工程、等を含む。
The general method for producing natural cheese is:
(1) a step of agglomerating raw milk to produce a coagulum and whey;
(2) cutting the coagulum and separating whey from (inside) the coagulum,
(3) including a process of discharging whey.
 これまでに知られている上述のナチュラルチーズの製造方法では、ホエイを排出する前、すなわち、上記工程(1)または(2)、もしくは、これらの直前または直後に、トランスグルタミナーゼを使用していた。これに対して、本発明では、ホエイを排出した後、すなわち、上記工程(3)の後に、トランスグルタミナーゼを使用する。なお、一般的な熟成型ナチュラルチーズの製造方法では、上記工程(3)の後に、チーズカードを熟成する工程を含む。そこで、本発明では、熟成型ナチュラルチーズを製造するためには、チーズカードを熟成する工程の前に、トランスグルタミナーゼを使用することとなる。 In the above-mentioned methods for producing natural cheese known so far, transglutaminase has been used before whey is discharged, that is, immediately before or after the above step (1) or (2). . On the other hand, in the present invention, transglutaminase is used after discharging whey, that is, after the step (3). In addition, in the manufacturing method of a general matured natural cheese, the process of ripening a cheese curd is included after the said process (3). Therefore, in the present invention, in order to produce ripened natural cheese, transglutaminase is used before the step of ripening the cheese curd.
 本発明の好ましい一態様において、トランスグルタミナーゼを添加した後、トランスグルタミナーゼが失活するような温度および時間で加熱する工程を含まないことが好ましい。これにより、最終製品や中間製品の熟成型ナチュラルチーズにおいて、トランスグルタミナーゼの活性が残存している。また、本発明の好ましい一態様において、トランスグルタミナーゼを添加した後、チーズカードを熟成する工程が完了する前に、トランスグルタミナーゼが失活するような温度および時間で加熱する工程を含まないことが好ましい。ここで、チーズカードを熟成する工程が完了した後に、トランスグルタミナーゼが失活するような温度および時間で加熱する工程を含むのであれば、最終製品や中間製品の熟成型ナチュラルチーズにおいて、トランスグルタミナーゼの活性が残存していなくても、熟成期間において、トランスグルタミナーゼが十分に作用し、最終製品や中間製品の熟成型ナチュラルチーズにおいて、耐熱保形性が良好に付与されているためである。なお、チーズカードを熟成する工程が完了するとは、最終製品や中間製品の熟成型ナチュラルチーズにおいて、所望の熟成風味が得られている段階を意味する。 In a preferred embodiment of the present invention, it is preferable not to include a step of heating at a temperature and a time at which transglutaminase is inactivated after adding transglutaminase. Thereby, the activity of transglutaminase remains in the matured natural cheese of the final product or intermediate product. Further, in a preferred embodiment of the present invention, it is preferable not to include a step of heating at a temperature and a time at which transglutaminase is inactivated after the addition of transglutaminase and before the step of ripening cheese curd is completed. . Here, after the step of ripening the cheese curd is completed, if it includes a step of heating at such a temperature and time that the transglutaminase is deactivated, in the matured natural cheese of the final product or intermediate product, transglutaminase This is because even when the activity does not remain, transglutaminase acts sufficiently during the ripening period, and heat-resistant shape retention is imparted well in the final product and the intermediate product of mature cheese. The completion of the process of ripening the cheese curd means a stage where a desired ripening flavor is obtained in the mature natural cheese of the final product or intermediate product.
 ここで、「トランスグルタミナーゼが失活するような温度および時間で加熱する」とは、トランスグルタミナーゼの種類や状態によって条件が異なり、商品の情報または当該技術分野における通常の実験により、それを容易に理解することができる。例えば、KS-CT(味の素(株)製)の場合には、65℃、2時間以上の加熱で失活し、より高温で短時間の加熱では、例えば、72℃以上、15秒以上の加熱で失活する。
 本発明において、トランスグルタミナーゼは、トランスグルタミナーゼの作用があるものであれば、その由来、酵素活性、精製度を任意に選択して使用することができる。例えば、市販のトランスグルタミナーゼ製剤を使用することができる。
Here, “heating at a temperature and time at which transglutaminase is inactivated” means that the conditions vary depending on the type and state of transglutaminase, which can be easily determined by product information or normal experiments in the art. I can understand. For example, in the case of KS-CT (manufactured by Ajinomoto Co., Inc.), it is deactivated by heating at 65 ° C. for 2 hours or more, and for heating at a higher temperature for a short time, for example, heating at 72 ° C. or more for 15 seconds or more. Deactivated.
In the present invention, as long as transglutaminase has the action of transglutaminase, its origin, enzyme activity, and degree of purification can be arbitrarily selected and used. For example, a commercially available transglutaminase preparation can be used.
 本発明において、チーズカードへトランスグルタミナーゼを添加する方法では、チーズカードにトランスグルタミナーゼが略均一に分散または混合されていればよく、その具体的な手段は限定されないが、例えば、チーズカードの粒に高濃度のトランスグルタミナーゼを添加してから、タンク(チーズバット)などで撹拌する方法、チーズカードの粒に高濃度のトランスグルタミナーゼを噴霧する方法、チーズカードの粒と高濃度のトランスグルタミナーゼをインライン(配管中)で流動させる方法などの一般的に知られた分散または混合する方法を用いることができる。 In the present invention, in the method of adding transglutaminase to cheese curd, transglutaminase may be dispersed or mixed substantially uniformly in cheese curd, and its specific means is not limited, but for example, in cheese curd grains After adding high-concentration transglutaminase, stirring in a tank (cheese vat), etc., spraying high-concentration transglutaminase onto cheese curd grains, in-line with cheese curd grains and high-concentration transglutaminase ( Generally known dispersion or mixing methods such as a method of flowing in a pipe) can be used.
 本発明において、トランスグルタミナーゼは、チーズカードを形成した後に添加されることが好ましい。チーズカードを形成する前、例えば、原料乳が凝乳する前にトランスグルタミナーゼが添加される、または原料乳が凝乳している途中にトランスグルタミナーゼが添加されると、チーズカードを形成する際に、チーズカードからホエイが十分に分離および排出されず、チーズカードを熟成する工程において離水を起こし、ナチュラルチーズ本来の品質を確保しにくいためである。 In the present invention, transglutaminase is preferably added after forming cheese curd. Before forming cheese curd, for example, when transglutaminase is added before the raw milk is curd, or when transglutaminase is added while the raw milk is curding, when forming the cheese curd This is because the whey is not sufficiently separated and discharged from the cheese curd, water separation occurs in the process of aging the cheese curd, and it is difficult to ensure the original quality of natural cheese.
 本発明の好ましい一態様において、原料乳を凝乳して得られた凝固物をカッティングし、ホエイを十分に排出した後に、この得られたチーズカードの粒にトランスグルタミナーゼを添加する。また、本発明の好ましい一態様において、ホエイのpHが5.8~6.4、好ましくは5.9~6.3、より好ましくは6.0~6.2程度となった時点で、ホエイを排出する。 In a preferred embodiment of the present invention, after cutting the coagulated product obtained by coagulating raw material milk and sufficiently discharging whey, transglutaminase is added to the obtained cheese curd grains. In one preferred embodiment of the present invention, when the pH of the whey reaches 5.8 to 6.4, preferably 5.9 to 6.3, more preferably about 6.0 to 6.2. Is discharged.
 本発明において、トランスグルタミナーゼの添加量は、任意であり、商品の情報または当該技術分野における通常の実験により、それを容易に調整することができる。ここで、例えば、トランスグルタミナーゼ濃度が1重量%、かつトランスグルタミナーゼ活性が86~130U/gのトランスグルタミナーゼ製剤を使用する場合に、トランスグルタミナーゼの添加量の下限値は、本発明の効果が得られる最低限の添加量となり、チーズカードの重量比(カード粒比)として、トランスグルタミナーゼ濃度が0.0002重量%以上、0.0003重量%以上、0.0004重量%以上、0.0005重量%以上、0.0006重量%以上、0.0007重量%以上、0.0008重量%以上、0.0009重量%以上、0.001重量%以上、0.0012重量%以上、0.0014重量%以上、0.0016重量%以上、0.0018重量%以上、0.002重量%以上であることが好ましい。このとき、トランスグルタミナーゼの効果を十分に発揮させる観点から、トランスグルタミナーゼの添加量の下限値は、上述の高い数値であることが好ましい。 In the present invention, the amount of transglutaminase added is arbitrary, and can be easily adjusted by information on products or a normal experiment in the technical field. Here, for example, when a transglutaminase preparation having a transglutaminase concentration of 1% by weight and a transglutaminase activity of 86 to 130 U / g is used, the lower limit of the amount of transglutaminase added can provide the effect of the present invention. The minimum addition amount, and the cheese curd weight ratio (curd grain ratio), the transglutaminase concentration is 0.0002 wt% or more, 0.0003 wt% or more, 0.0004 wt% or more, 0.0005 wt% or more 0.0006 wt% or more, 0.0007 wt% or more, 0.0008 wt% or more, 0.0009 wt% or more, 0.001 wt% or more, 0.0012 wt% or more, 0.0014 wt% or more, It is preferably 0.0016% by weight or more, 0.0018% by weight or more, or 0.002% by weight or more. At this time, from the viewpoint of sufficiently exhibiting the effect of transglutaminase, the lower limit value of the addition amount of transglutaminase is preferably the above-described high numerical value.
 一方、例えば、トランスグルタミナーゼ濃度が1重量%、かつトランスグルタミナーゼ活性が86~130U/gのトランスグルタミナーゼ製剤をチーズカードに添加する場合に、トランスグルタミナーゼの添加量の上限値は、本発明の効果が得られる最大限の添加量となり、チーズカードの重量比(カード粒比)として、トランスグルタミナーゼ濃度が10重量%以下、5重量%以下、1重量%以下、0.1重量%以下、0.05重量%以下、0.01重量%以下、0.008重量%以下、0.006重量%以下、0.005重量%以下、0.004重量%以下、0.003重量%以下である。このとき、トランスグルタミナーゼの添加量が多すぎると、ナチュラルチーズが非常に硬くなる傾向があるため、耐熱保形性の効果に加えて、食感などを適度に調整する観点から、トランスグルタミナーゼの添加量の上限値は、上述の低い数値であることが好ましい。なお、これらの下限値および上限値は適宜、それぞれを組み合わせて設定することができる。また、トランスグルタミナーゼ濃度、およびトランスグルタミナーゼ活性が異なるトランスグルタミナーゼ製剤を使用する場合に、その必要なトランスグルタミナーゼの添加量は、それぞれの活性が同等となるように計算して設定することが可能である。 On the other hand, for example, when a transglutaminase preparation having a transglutaminase concentration of 1% by weight and a transglutaminase activity of 86 to 130 U / g is added to cheese curd, the upper limit of the amount of transglutaminase added is the effect of the present invention. The maximum amount obtained is obtained, and as a weight ratio (curd grain ratio) of cheese curd, the transglutaminase concentration is 10% by weight or less, 5% by weight or less, 1% by weight or less, 0.1% by weight or less, 0.05 % By weight or less, 0.01% by weight or less, 0.008% by weight or less, 0.006% by weight or less, 0.005% by weight or less, 0.004% by weight or less, 0.003% by weight or less. At this time, if the amount of transglutaminase added is too large, natural cheese tends to become very hard, so in addition to the effect of heat-resistant shape retention, the addition of transglutaminase The upper limit of the amount is preferably the above-mentioned low numerical value. Note that these lower limit value and upper limit value can be appropriately set in combination. In addition, when transglutaminase preparations having different transglutaminase concentrations and transglutaminase activities are used, the necessary addition amount of transglutaminase can be calculated and set so that the respective activities are equivalent. .
 本発明において、トランスグルタミナーゼ濃度が1重量%、かつトランスグルタミナーゼ活性が86~130U/gのトランスグルタミナーゼ製剤を使用する場合に、例えば、トランスグルタミナーゼの添加量は、チーズカードの重量比(カード粒比)として、具体的には、トランスグルタミナーゼ濃度が0.0002~10重量%、0.0003~10重量%、0.0004~5重量%、0.0005~5重量%、0.0006~1重量%、0.0007~1重量%、0.0008~0.1重量%、0.0009~0.05重量%、0.001~0.01重量%、0.0012~0.008重量%、0.0014~0.006重量%、0.0016~0.005重量%、0.0018~0.004重量%、0.002~0.003重量%であることが好ましい。 In the present invention, when a transglutaminase preparation having a transglutaminase concentration of 1% by weight and a transglutaminase activity of 86 to 130 U / g is used, for example, the amount of transglutaminase added is the cheese curd weight ratio (curd grain ratio). ), Specifically, the transglutaminase concentration is 0.0002 to 10% by weight, 0.0003 to 10% by weight, 0.0004 to 5% by weight, 0.0005 to 5% by weight, 0.0006 to 1% by weight. %, 0.0007 to 1% by weight, 0.0008 to 0.1% by weight, 0.0009 to 0.05% by weight, 0.001 to 0.01% by weight, 0.0012 to 0.008% by weight, 0.0014 to 0.006 wt%, 0.0016 to 0.005 wt%, 0.0018 to 0.004 wt%, 0.002 to 0.003% wt% There it is preferable.
 本発明において、必要に応じて、チーズカードに加塩することができる。このとき、チーズカードに加塩する時期は、チーズカードにトランスグルタミナーゼを添加する時期と同時でもよいし、チーズカードにトランスグルタミナーゼを添加した後に、チーズカードを成型して熟成する前でもよい。また、チーズカードに加塩する方法は、ブライン(塩水)へ浸漬させる方法などの公知の方法を用いることができる。 In the present invention, the cheese curd can be salted as necessary. At this time, the time for salting the cheese curd may be the same as the time for adding transglutaminase to the cheese curd, or after adding the transglutaminase to the cheese curd and before ripening by shaping the cheese curd. Moreover, the method of salting a cheese curd can use well-known methods, such as the method of immersing in a brine (brine).
 本発明の好ましい一態様において、チーズカードを熟成する工程を設けて、熟成型ナチュラルチーズを製造することができる。このとき、本発明において、チーズカードを熟成するとは、通常のナチュラルチーズにおける熟成を意味し、熟成型のナチュラルチーズの製造方法における通常の公知の条件を用いることができる。そして、熟成型のナチュラルチーズにおいて公知の条件とは、例えば、温度が4~20℃、湿度が50~80%に保持することである。ここで、本発明において、実際に目的とする熟成型ナチュラルチーズの製造方法における公知の条件を用いることができ、例えば、4℃、7℃、15℃、20℃などの適切な温度を下限値および上限値として、それぞれ選択して設定することができる。また、本発明において、実際に目的とする熟成型ナチュラルチーズの製造方法における公知の条件を用いることができ、例えば、50%、60%、70%、80%、90%などの適切な湿度を下限値および上限値として、それぞれ選択して設定することができる。 In a preferred embodiment of the present invention, a matured natural cheese can be produced by providing a step of aging a cheese curd. At this time, in the present invention, ripening a cheese curd means aging in normal natural cheese, and normal known conditions in a method for producing a mature natural cheese can be used. The known conditions for mature natural cheese are, for example, that the temperature is kept at 4 to 20 ° C. and the humidity is kept at 50 to 80%. Here, in this invention, the well-known conditions in the manufacturing method of the matured natural cheese actually made into the objective can be used, for example, suitable temperature, such as 4 degreeC, 7 degreeC, 15 degreeC, and 20 degreeC, is a lower limit. And the upper limit value can be selected and set. Moreover, in this invention, the well-known conditions in the manufacturing method of the matured natural cheese actually intended can be used, for example, appropriate humidity, such as 50%, 60%, 70%, 80%, 90%, etc. Each of the lower limit value and the upper limit value can be selected and set.
 本発明において、チーズカードを熟成する期間は、実際に目的とする熟成型ナチュラルチーズの製造方法における公知の条件を用いることができる。つまり、本発明の効果である耐熱保形性が得られれば、チーズカードを熟成する期間は、特に限定されないが、トランスグルタミナーゼの作用が十分に発揮され、ナチュラルチーズの耐熱保形性を向上する観点から、1日以上、好ましくは2日以上、より好ましくは1週間以上、さらに好ましくは2週間以上、さらに好ましくは1ヶ月以上である。また、チーズカードを熟成する期間は、ナチュラルチーズの熟成風味を得る観点から、1年以上、好ましくは1.5年以上、より好ましくは2年以上、さらに好ましくは3年以上である。 In the present invention, during the ripening period of the cheese curd, known conditions in the actual method for producing a mature natural cheese can be used. That is, if the heat-resistant shape retention which is the effect of the present invention is obtained, the period for ripening the cheese curd is not particularly limited, but the action of transglutaminase is sufficiently exerted to improve the heat-resistant shape retention of natural cheese. From the viewpoint, it is 1 day or more, preferably 2 days or more, more preferably 1 week or more, still more preferably 2 weeks or more, and further preferably 1 month or more. Moreover, the period which ripens a cheese curd is 1 year or more from the viewpoint of obtaining the ripening flavor of natural cheese, Preferably it is 1.5 years or more, More preferably, it is 2 years or more, More preferably, it is 3 years or more.
 本発明において、チーズカードを熟成する期間は、本発明の効果である耐熱保形性が得られれば、特に限定されないが、具体的には、1日間~2年間、2日間~1年間、1週間~6ヶ月間、2週間~4ヶ月間、1ヶ月間~4ヶ月間であることが好ましい。つまり、本発明において、チーズカードを熟成する条件および期間は、一般的なナチュラルチーズの製造方法で用いられる条件および期間を採用することができ、適宜、それぞれを選択して設定することができる。本発明の好ましい一態様において、熟成型ナチュラルチーズを製造するために、4~20℃、1ヶ月以上、より好ましくは4~20℃、1~4ヶ月間で熟成することが好ましい。 In the present invention, the ripening period of the cheese curd is not particularly limited as long as the heat resistant shape retention which is the effect of the present invention can be obtained, but specifically, 1 day to 2 years, 2 days to 1 year, It is preferable that the period is from week to 6 months, from 2 weeks to 4 months, and from 1 month to 4 months. That is, in this invention, the conditions and period used by the manufacturing method of a general natural cheese can be employ | adopted for the conditions and period which ripen a cheese curd, and can select and set each suitably. In a preferred embodiment of the present invention, it is preferable to ripen at 4 to 20 ° C. for 1 month or more, more preferably at 4 to 20 ° C. for 1 to 4 months, in order to produce mature natural cheese.
 本発明は、熟成工程を要する全部のナチュラルチーズに応用することができる。すなわち、本発明は、ゴーダ、チェダー、パルメザンなどの熟成型ナチュラルチーズ、カマンベール、ブリーなどの白カビチーズ、ゴルゴンゾーラ、ロックフォールなどの青カビチーズを例示できるが、これら例示した以外の熟成工程を要するナチュラルチーズの全部に応用することができる。したがって、本発明のナチュラルチーズの製造方法は、熟成工程を要するナチュラルチーズの製造方法に用いることができる。 The present invention can be applied to all natural cheeses that require a ripening process. That is, the present invention can be exemplified by mature cheeses such as gouda, cheddar and parmesan, white mold cheeses such as camembert and brie, and blue mold cheeses such as gorgonzola and roquefort, but natural cheeses which require a ripening step other than those exemplified above. It can be applied to all of the above. Therefore, the manufacturing method of the natural cheese of this invention can be used for the manufacturing method of the natural cheese which requires a ripening process.
 本発明のナチュラルチーズには、耐熱保形性に大きく影響しない限り、製品の設計などを勘案し、必要に応じて、任意の食品原料および/または食品添加物を添加することができる。 The natural cheese of the present invention can be added with any food material and / or food additive as necessary, taking into consideration the design of the product, as long as it does not significantly affect the heat-resistant shape retention.
 本発明のナチュラルチーズは、耐熱保形性を有する。本発明において「耐熱保形性を有する」とは、後述の測定法(試験法)によって評価(判断)した結果が所定値以上であることをいう。すなわち、この耐熱保形性の測定法では、例えば、試験例に示すように、ナチュラルチーズを13mm四方に切り出し、この切り出したナチュラルチーズを設置し、一定条件で加熱(焼成)し、加熱後に冷却したナチュラルチーズの高さを測定し、加熱前のナチュラルチーズの高さ(13mm)を基準にして、以下の計算式に基づき、耐熱保形性を算出する。
(耐熱保形性)(%)=(加熱後のチーズの高さ)(mm)/(加熱前のチーズの高さ)(13mm)×100
The natural cheese of the present invention has heat-resistant shape retention. In the present invention, “having heat-resistant shape retention” means that the result of evaluation (judgment) by a measurement method (test method) described later is a predetermined value or more. That is, in this heat-resistant shape retention measurement method, for example, as shown in a test example, natural cheese is cut into a 13 mm square, the cut natural cheese is installed, heated (baked) under certain conditions, and cooled after heating. The height of the natural cheese is measured, and the heat-resistant shape retention property is calculated based on the following formula based on the height (13 mm) of the natural cheese before heating.
(Heat-resistant shape retention) (%) = (height of cheese after heating) (mm) / (height of cheese before heating) (13 mm) × 100
 この耐熱保形性の測定法では、乾熱下と湿熱下の2種類の焼成(加熱)を利用することができる。このとき、乾熱下の焼成(乾熱加熱時)の耐熱保形性では、アルミトレーにアルミ箔を敷き、ナチュラルチーズを切り出して設置する。そして、220℃、3分間で加熱した後に、室温(約20℃)、3分間で放冷して評価する。また、湿熱下の焼成(湿熱加熱時)の耐熱保形性では、シャーレに濾紙を敷き、水1mlを滴下して、ナチュラルチーズを切り出して設置してから、シャーレの蓋を閉じる。そして、130℃、20分間で加熱した後に、室温(約20℃)、3分間で放冷して評価する。なお、本発明の耐熱保形性では、例えば、乾熱加熱時に50%以上、湿熱加熱時に40%以上であれば、耐熱保形性を有すると評価することができる。そこで、本発明の好ましい一態様において、ナチュラルチーズの耐熱保形性は、乾熱加熱時に50%以上、好ましくは60%以上、より好ましくは70%以上、さらに好ましくは80%である。また、本発明の好ましい一態様において、ナチュラルチーズの耐熱保形性は、湿熱加熱時に40%以上、好ましくは50%以上、より好ましくは60%以上、さらに好ましくは70%である。 This measurement method for heat-resistant shape retention can use two types of firing (heating) under dry heat and wet heat. At this time, in heat-resistant shape retention during baking under dry heat (during dry heat heating), an aluminum foil is laid on an aluminum tray, and natural cheese is cut out and installed. And after heating at 220 degreeC for 3 minutes, it cools and evaluates at room temperature (about 20 degreeC) for 3 minutes. Moreover, in heat-resistant shape retention of baking under wet heat (at the time of wet heat heating), filter paper is spread on the petri dish, 1 ml of water is dropped, natural cheese is cut out and installed, and then the lid of the petri dish is closed. And after heating at 130 degreeC for 20 minutes, it cools and evaluates at room temperature (about 20 degreeC) for 3 minutes. In addition, in the heat-resistant shape retention of this invention, if it is 50% or more at the time of dry heat heating and 40% or more at the time of wet heat heating, it can be evaluated that it has heat-resistant shape retention. Therefore, in a preferred embodiment of the present invention, the heat resistant shape retention of natural cheese is 50% or more, preferably 60% or more, more preferably 70% or more, and even more preferably 80% during dry heat heating. Moreover, in one preferable aspect of the present invention, the heat resistant shape retention of natural cheese is 40% or more, preferably 50% or more, more preferably 60% or more, and even more preferably 70% during wet heat heating.
 本発明において、乾熱下の焼成の耐熱保形性は、チーズをパンなどの表面に置いてオーブンなどで焼成した場合を想定している。すなわち、本発明において、乾熱下の耐熱保形性の測定(評価)では、チーズを加熱する温度は、例えば、150~250℃、170~250℃、180~250℃、200~250℃、200~240℃、210~230℃である。そして、チーズを加熱する保持時間は、例えば、5~20分間、5~18分間、7~17分間、8~16分間、9~15分間、10~14分間、11~13分間である。なお、これら加熱する温度および保持時間は、チーズの用途などに合わせて適宜、それぞれを組み合わせて設定することができる。 In the present invention, the heat-resistant shape retention of baking under dry heat assumes that cheese is placed on the surface of bread or the like and baked in an oven or the like. That is, in the present invention, in the measurement (evaluation) of heat-resistant shape retention under dry heat, the temperature for heating the cheese is, for example, 150 to 250 ° C, 170 to 250 ° C, 180 to 250 ° C, 200 to 250 ° C, 200 to 240 ° C. and 210 to 230 ° C. The holding time for heating the cheese is, for example, 5 to 20 minutes, 5 to 18 minutes, 7 to 17 minutes, 8 to 16 minutes, 9 to 15 minutes, 10 to 14 minutes, or 11 to 13 minutes. In addition, these heating temperature and holding time can be set combining each suitably according to the use etc. of cheese.
 本発明において、乾熱下の焼成の耐熱保形性の測定では、チーズを加熱する温度は、高温の耐熱保形性を効果的に評価する観点から、例えば、150℃以上、好ましくは170℃以上、より好ましくは180℃以上、さらに好ましくは200℃以上、さらに好ましくは210℃以上であり、実際の調理温度を想定すると、例えば、250℃以下、好ましくは240℃以下、より好ましくは230℃以下であり、これらの下限値および上限値は適宜、それぞれを組み合わせて設定することができる。また、本発明において、乾熱下の焼成の耐熱保形性の測定では、チーズを加熱する保持時間は、チーズを加熱する温度に依存して適宜選択して設定できるが、実際の調理温度を想定すると、例えば、5分以上、好ましくは7分以上、より好ましくは8分以上、さらに好ましくは9分以上、さらに好ましくは10分以上、さらに好ましくは11分以上であり、短時間で測定する観点から、例えば、20分以下、好ましくは18分以下、より好ましくは17分以下、さらに好ましくは16分以下、さらに好ましくは15分以下、さらに好ましくは14分以下、さらに好ましくは13分以下であり、これらの下限値および上限値は適宜、それぞれを組み合わせて設定することができる。 In the present invention, in the measurement of heat-resistant shape retention of baking under dry heat, the temperature at which the cheese is heated is, for example, 150 ° C. or higher, preferably 170 ° C. from the viewpoint of effectively evaluating high-temperature heat-resistant shape retention properties. Above, more preferably 180 ° C. or higher, further preferably 200 ° C. or higher, more preferably 210 ° C. or higher, and assuming an actual cooking temperature, for example, 250 ° C. or lower, preferably 240 ° C. or lower, more preferably 230 ° C. The lower limit value and the upper limit value can be appropriately set in combination. In the present invention, in the measurement of heat-resistant shape retention of baking under dry heat, the holding time for heating the cheese can be appropriately selected and set depending on the temperature for heating the cheese, but the actual cooking temperature is Assuming, for example, 5 minutes or more, preferably 7 minutes or more, more preferably 8 minutes or more, more preferably 9 minutes or more, still more preferably 10 minutes or more, and even more preferably 11 minutes or more. From the viewpoint, for example, 20 minutes or less, preferably 18 minutes or less, more preferably 17 minutes or less, more preferably 16 minutes or less, further preferably 15 minutes or less, further preferably 14 minutes or less, and further preferably 13 minutes or less. Yes, these lower limit value and upper limit value can be appropriately set in combination.
 本発明のナチュラルチーズは、上述したような温度および保持時間において、乾熱下の焼成の耐熱保形性を有するので、ピザ、グラタンなどのように、加熱調理食品などの表面にナチュラルチーズを載せて加熱する材料として使用することに適している。したがって、本発明は、本発明のナチュラルチーズを含む加熱調理食品にも関する。ここで、加熱調理とは、対象物の温度を上昇させる調理であれば、特に限定されず、焼く、煮る、蒸す、茹でる、炒める、炊く、揚げるなどの調理を挙げることができる。本発明のナチュラルチーズは、乾熱下の焼成の耐熱保形性を有することから、焼く加熱調理食品に用いることに適している。 Since the natural cheese of the present invention has heat-resistant shape retention of baking under dry heat at the temperature and holding time described above, natural cheese is placed on the surface of cooked food such as pizza and gratin. It is suitable for use as a heating material. Therefore, this invention relates also to the cooked food containing the natural cheese of this invention. Here, the heat cooking is not particularly limited as long as the temperature of the object is raised, and includes cooking such as baking, boiling, steaming, boiling, frying, cooking, and frying. Since the natural cheese of the present invention has heat-resistant shape retention after baking under dry heat, it is suitable for use in cooked cooked foods.
 本発明において、湿熱下の焼成の耐熱保形性は、チーズをパンなどに包餡してから、オーブンなどで焼成した場合を想定している。すなわち、湿熱下の焼成の耐熱保形性の測定(評価)では、チーズを加熱する温度は、例えば、100~200℃、100~180℃、100~170℃、100~160℃、110~150℃、120~140℃である。そして、チーズを加熱する保持時間は、例えば、5~40分間、10~30分間、12~28分間、15~25分間、16~24分間、17~23分間、18~22分間である。なお、これら加熱する温度および焼成時間は、チーズの用途に合わせて適宜、それぞれを組み合わて設定することができる。 In the present invention, the heat-resistant shape retention of baking under wet heat assumes that cheese is wrapped in bread or the like and then baked in an oven or the like. That is, in the measurement (evaluation) of heat-resistant shape retention of baking under wet heat, the temperature at which the cheese is heated is, for example, 100 to 200 ° C., 100 to 180 ° C., 100 to 170 ° C., 100 to 160 ° C., 110 to 150 ° C, 120-140 ° C. The holding time for heating the cheese is, for example, 5 to 40 minutes, 10 to 30 minutes, 12 to 28 minutes, 15 to 25 minutes, 16 to 24 minutes, 17 to 23 minutes, or 18 to 22 minutes. In addition, these heating temperature and baking time can be set combining each suitably according to the use of cheese.
 本発明の一態様において、湿熱下の焼成の耐熱保形性の測定では、チーズを加熱する温度は、高温の耐熱保形性を効果的に評価する観点から、例えば、100℃以上、好ましくは110℃以上、より好ましくは120℃以上であり、実際の調理温度を想定すると、例えば、200℃以下、好ましくは180℃以下、より好ましくは170℃以下、さらに好ましくは160℃以下、さらに好ましくは150℃以下、さらに好ましくは140℃以下であり、これらの下限値および上限値は適宜、それぞれを組み合わせて設定することができる。また、本発明において、湿熱下の焼成の耐熱保形性の測定では、チーズを加熱する保持時間は、チーズを加熱する温度に依存して適宜選択して設定できるが、実際の調理温度を想定すると、例えば、5分以上、好ましくは10分以上、より好ましくは12分以上、さらに好ましくは15分以上、さらに好ましくは16分以上、さらに好ましくは17分以上であり、さらに好ましくは18分以上であり、短時間で測定する観点から、例えば、28分以下、好ましくは25分以下、より好ましくは24分以下、さらに好ましくは23分以下、さらに好ましくは22分以下であり、これらの下限値および上限値は適宜、それぞれを組み合わせて設定することができる。 In one embodiment of the present invention, in the measurement of heat-resistant shape retention of baking under wet heat, the temperature at which the cheese is heated is, for example, 100 ° C. or higher, preferably from the viewpoint of effectively evaluating high-temperature heat-resistant shape retention properties. 110 ° C. or higher, more preferably 120 ° C. or higher, and assuming an actual cooking temperature, for example, 200 ° C. or lower, preferably 180 ° C. or lower, more preferably 170 ° C. or lower, more preferably 160 ° C. or lower, more preferably It is 150 ° C. or lower, more preferably 140 ° C. or lower, and these lower limit value and upper limit value can be appropriately set in combination. In the present invention, in the measurement of heat-resistant shape retention of baking under wet heat, the holding time for heating the cheese can be appropriately selected and set depending on the temperature for heating the cheese, but the actual cooking temperature is assumed. Then, for example, 5 minutes or more, preferably 10 minutes or more, more preferably 12 minutes or more, further preferably 15 minutes or more, further preferably 16 minutes or more, further preferably 17 minutes or more, and further preferably 18 minutes or more. From the viewpoint of measuring in a short time, it is, for example, 28 minutes or less, preferably 25 minutes or less, more preferably 24 minutes or less, still more preferably 23 minutes or less, and even more preferably 22 minutes or less. The upper limit value can be set in combination as appropriate.
 本発明のナチュラルチーズは、上述したような温度および保持時間において、湿熱下の焼成の耐熱保形性を有するので、例えば、パン、まんじゅう、ギョウザ、シュウマイ、春巻きなどのように、加熱調理食品などの内部にナチュラルチーズを包餡して加熱する材料として使用することに適している。したがって、本発明は、本発明のナチュラルチーズを内包して含む加熱調理食品にも関する。ここで、上述した通り、加熱調理とは、対象物の温度を上昇させる調理であれば、特に限定されず、焼く、煮る、蒸す、茹でる、炒める、炊く、揚げるなどの調理を挙げることができる。本発明のナチュラルチーズは、湿熱下の焼成の耐熱保形性を有することから、煮る、蒸す、茹でる加熱調理食品に用いることに適している。 Since the natural cheese of the present invention has heat-resistant shape retention under baking at the temperature and holding time described above, for example, cooked food such as bread, manju, gyoza, shumai, spring rolls, etc. It is suitable for use as a material to heat natural cheese wrapped inside. Therefore, the present invention also relates to a cooked food containing the natural cheese of the present invention. Here, as described above, the heating cooking is not particularly limited as long as it is a cooking that raises the temperature of the object, and includes cooking such as baking, boiling, steaming, boiling, frying, cooking, and frying. . The natural cheese of the present invention is suitable for use in cooked and cooked foods that are boiled, steamed, and boiled because it has heat-resistant shape retention in baking under wet heat.
 本発明の一形態は、150~250℃、5~20分間の乾熱下で焼成して耐熱保形性を有すること、および/または100~200℃、5~40分間の湿熱下で焼成して耐熱保形性を有することを特徴とする、耐熱保形性を有するナチュラルチーズの評価方法である。 One embodiment of the present invention has heat-resistant shape retention by baking at 150 to 250 ° C. for 5 to 20 minutes and / or baking at 100 to 200 ° C. for 5 to 40 minutes. It is a method for evaluating natural cheese having heat-resistant shape retention, characterized by having heat-resistant shape retention.
 また、本発明の一形態は、150~250℃、5~20分間の乾熱で焼成して、50%以上の耐熱保形性を有すること、および/または100~200℃、5~40分間の湿熱下で焼成して、40%以上の耐熱保形性を有することを特徴とする、耐熱保形性を有するナチュラルチーズの評価方法である。 Further, according to one embodiment of the present invention, the heat-resistant shape retention of 50% or more by baking at 150 to 250 ° C. for 5 to 20 minutes and / or 100 to 200 ° C. for 5 to 40 minutes It is the evaluation method of the natural cheese which has heat-resistant shape retention characterized by having baked under the wet heat of and having 40% or more heat-resistant shape retention property.
 本発明は、ホエイを分離して排出して得られたチーズカードに、トランスグルタミナーゼを添加することを特徴とし、トランスグルタミナーゼを添加する以外では、一般的なナチュラルチーズ、特に、一般的な熟成型ナチュラルチーズの製造工程を利用することができ、トランスグルタミナーゼ以外の原料では、一般的なナチュラルチーズの製造方法で用いられる原料を利用することができる。 The present invention is characterized by adding transglutaminase to cheese curd obtained by separating and discharging whey, except for adding transglutaminase, general natural cheese, particularly general ripening A natural cheese manufacturing process can be used, and raw materials other than transglutaminase can be used raw materials used in general natural cheese manufacturing methods.
実施例
 以下では、実施例を挙げて、本発明をさらに詳細に説明するが、本発明は、これにより限定されない。
EXAMPLES Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.
[試験例1]
 製品ナチュラルチーズの固形分中の脂肪濃度が45重量%となるように、原料乳を調製し、63℃、30分間で加熱殺菌した後に、33℃に冷却してから、塩化カルシウム(0.03重量%)を添加した。次に、これらの原料乳に、チーズ製造用の市販スターター(L. Lactis ダニスコ社製)を0.1%添加した。これらスターターを添加した後に、カーフレンネット(力価:15000ユニット)を0.003重量%で添加し、原料乳を凝固させた。この凝固物をカッティングしホエイのpHが6.1~6.2となるまで攪拌して、ホエイを排出しカード粒を得た。
[Test Example 1]
The raw material milk is prepared so that the fat concentration in the solid content of the product natural cheese is 45% by weight, sterilized by heating at 63 ° C. for 30 minutes, cooled to 33 ° C., and then calcium chloride (0.03 % By weight) was added. Next, 0.1% of a commercial starter for cheese production (manufactured by L. Lactis Danisco) was added to these raw milks. After adding these starters, calf rennet (titer: 15000 units) was added at 0.003% by weight to coagulate the raw milk. The coagulated product was cut and stirred until the whey had a pH of 6.1 to 6.2, and the whey was discharged to obtain curd grains.
 次に、このカード粒に食塩を所定量で添加してから、20重量%のトランスグルタミナーゼ製剤水溶液をカード粒比1重量%で添加し、型詰めして圧搾し、ナチュラルチーズ(水分40.4重量%、乳脂肪分45.3重量%、塩分1.4重量%、pH5.23)を製造した。トランスグルタミナーゼ製剤としてはKS-CT(味の素(株)製:トランスグルタミナーゼ濃度1%、トランスグルタミナーゼ活性86~130U/g)を使用した。トランスグルタミナーゼは、チーズカードに対して(カード粒比)0.002重量%添加した。また、同時にトランスグルタミナーゼを添加しないナチュラルチーズを上記と同様の方法で試作した。
 これらのナチュラルチーズを4、7、12または20℃で熟成し、熟成4ヶ月間における耐熱保形性を測定した。ここで、トランスグルタミナーゼを添加した系を実施例1(4℃熟成)、実施例2(7℃熟成)、実施例3(12℃熟成)、実施例4(20℃熟成)と称し、トランスグルタミナーゼを添加しない系を比較例1(4℃熟成)、比較例2(12℃熟成)と称した。
Next, after adding a predetermined amount of salt to this curd grain, 20% by weight of a transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, stuffed and squeezed, and natural cheese (water content 40.4%) is added. % By weight, milk fat content 45.3% by weight, salt content 1.4% by weight, pH 5.23). As a transglutaminase preparation, KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used. Transglutaminase was added in an amount of 0.002% by weight to the cheese curd (curd grain ratio). At the same time, a natural cheese to which no transglutaminase was added was produced in the same manner as described above.
These natural cheeses were ripened at 4, 7, 12 or 20 ° C., and the heat-resistant shape retention during 4 months of aging was measured. Here, the system to which transglutaminase was added was referred to as Example 1 (aged at 4 ° C.), Example 2 (aged at 7 ° C.), Example 3 (aged at 12 ° C.), and Example 4 (aged at 20 ° C.). The system in which no was added was referred to as Comparative Example 1 (aged at 4 ° C.) and Comparative Example 2 (aged at 12 ° C.).
[耐熱保形性の評価]
 耐熱保形性は以下の方法で測定し、数値化を行った。耐熱保形性は乾熱加熱と湿熱加熱の両方を測定した。
[乾熱加熱]
 ナチュラルチーズを13mm×13mm×13mmのサンプルに切り出す。このサンプルの1種類について3個ずつで測定する。オーブン(SANSYO DRYING OVEN SDW27)を220℃に設定する。アルミトレーにアルミ箔を敷き、サンプルの3個を横一列に等間隔で並べる。アルミトレーごとでサンプルの3個をオーブンに入れて12分間で加熱する。加熱後にアルミトレーごとでサンプルの3個をオーブンから取り出して室温(常温)に置いて3分間で放冷する。放冷後にサンプルの高さをノギスで測定して記録する。加熱前と加熱後のサンプルの高さから、耐熱保形性を計算する。
 ここで、耐熱保形性を以下の通りに算出し、乾熱加熱時の耐熱保形性が50%以上の場合、耐熱保形性があると評価した。
耐熱保形性(%)=加熱後のチーズの高さ/加熱前のチーズの高さ×100
[Evaluation of heat-resistant shape retention]
The heat-resistant shape retention was measured by the following method and digitized. For heat-resistant shape retention, both dry heat and wet heat were measured.
[Dry heat heating]
Cut natural cheese into 13 mm x 13 mm x 13 mm samples. One sample is measured in triplicate. Set the oven (SANSYO DRYING OVEN SDW27) to 220 ° C. Lay the aluminum foil on the aluminum tray and arrange the three samples in a horizontal row at equal intervals. Three samples of each aluminum tray are placed in an oven and heated for 12 minutes. After heating, three samples of each aluminum tray are removed from the oven, placed at room temperature (room temperature), and allowed to cool for 3 minutes. After standing to cool, measure the sample height with calipers and record. Heat resistant shape retention is calculated from the height of the sample before and after heating.
Here, the heat-resistant shape retention was calculated as follows, and when the heat-resistant shape retention during dry heat heating was 50% or more, it was evaluated that there was heat-resistant shape retention.
Heat-resistant shape retention (%) = height of cheese after heating / height of cheese before heating × 100
[湿熱加熱]
 ナチュラルチーズを13mm×13mm×13mmのサンプルに切り出す。このサンプルの1種類について3個ずつで測定する。オーブン(SANSYO DRYING OVEN SDW27)を130℃に設定する。ガラスシャーレに濾紙(TOYO 2番 70mm)を敷き、濾紙に水1mlを塗布してから、サンプルの3個を三角形状に等間隔で並べてから、ガラスシャーレの蓋を閉じる。ガラスシャーレごとでサンプルの3個をオーブンに入れて20分間で加熱する。加熱後にガラスシャーレごとでサンプルの3個をオーブンから取り出して室温(常温)に置いて3分間で放冷する。放冷後にサンプルの高さをノギスで測定して記録する。加熱前と加熱後のサンプルの高さから、耐熱保形性を計算する。
 ここで、耐熱保形性を以下の通りに算出し、湿熱加熱時の耐熱保形性が40%以上の場合、耐熱保形性があると評価した。
耐熱保形性(%)=加熱後のチーズの高さ/加熱前のチーズの高さ×100
[Humid heat]
Cut natural cheese into 13 mm x 13 mm x 13 mm samples. One sample is measured in triplicate. Set the oven (SANSYO DRYING OVEN SDW27) to 130 ° C. A filter paper (TOYO No. 2 70 mm) is spread on the glass petri dish, 1 ml of water is applied to the filter paper, three samples are arranged in a triangular shape at equal intervals, and the lid of the glass petri dish is closed. Three samples of each glass petri dish are placed in an oven and heated for 20 minutes. After heating, three samples of each glass petri dish are taken out of the oven, placed at room temperature (room temperature) and allowed to cool for 3 minutes. After standing to cool, measure the sample height with calipers and record. Heat resistant shape retention is calculated from the height of the sample before and after heating.
Here, the heat-resistant shape retention was calculated as follows, and when the heat-resistant shape retention during wet heat heating was 40% or more, it was evaluated that there was heat-resistant shape retention.
Heat-resistant shape retention (%) = height of cheese after heating / height of cheese before heating × 100
[耐熱保形性の評価]
 表1~2の通り、熟成後に耐熱保形性を測定した結果、トランスグルタミナーゼを0.002%添加した実施例1~4では、熟成4ヶ月後で、耐熱保形性を有していた。一方、トランスグルタミナーゼを添加しない比較例1~2では、熟成4ヶ月後で、耐熱保形性を有していなかった。
[Evaluation of heat-resistant shape retention]
As shown in Tables 1 and 2, as a result of measuring the heat retaining shape after aging, Examples 1 to 4 to which 0.002% of transglutaminase was added had heat retaining shape after 4 months of aging. On the other hand, Comparative Examples 1 and 2 to which no transglutaminase was added did not have heat-resistant shape retention after 4 months of aging.
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000002
Figure JPOXMLDOC01-appb-T000002
[試験例2]
 製品ナチュラルチーズの固形分中の脂肪濃度が47重量%となるように、原料乳を調製し、63℃、30分間で加熱殺菌した後に、33℃に冷却してから、塩化カルシウム(0.03重量%)を添加した。次に、これらの原料乳に、チーズ製造用の市販スターター(L. Lactis ダニスコ社製)を0.1%添加した。これらスターターを添加した後に、カーフレンネット(力価:15000ユニット)を0.003重量%で添加し、原料乳を凝固させた。この凝固物をカッティングしホエイのpHが6.1~6.2となるまで攪拌して、ホエイを排出しカード粒を得た。
[Test Example 2]
The raw milk is prepared so that the fat concentration in the solid content of the product natural cheese is 47% by weight, sterilized by heating at 63 ° C. for 30 minutes, cooled to 33 ° C., and then calcium chloride (0.03 % By weight) was added. Next, 0.1% of a commercial starter for cheese production (manufactured by L. Lactis Danisco) was added to these raw milks. After adding these starters, calf rennet (titer: 15000 units) was added at 0.003% by weight to coagulate the raw milk. The coagulated product was cut and stirred until the whey had a pH of 6.1 to 6.2, and the whey was discharged to obtain curd grains.
 次に、このカード粒に食塩を所定量で添加してから、20重量%のトランスグルタミナーゼ製剤水溶液をカード粒比1重量%で添加し、型詰めして圧搾し、ナチュラルチーズ(水分39.2重量%、乳脂肪分47.1重量%、塩分1.45重量%、pH5.39)を製造した。トランスグルタミナーゼ製剤としてはKS-CT(味の素(株)製:トランスグルタミナーゼ濃度1%、トランスグルタミナーゼ活性86~130U/g)を使用した。
トランスグルタミナーゼは、カード粒比で0.002重量%添加した。また、同時にトランスグルタミナーゼを添加しないナチュラルチーズを上記と同様の方法で試作した。
 これらのナチュラルチーズを12℃で熟成し、熟成1ヶ月間、2ヶ月間、3ヶ月間、4ヶ月間における耐熱保形性を試験例1と同様の方法で測定した。ここで、トランスグルタミナーゼを添加した系を実施例5(12℃熟成)と称し、トランスグルタミナーゼを添加しない系を比較例3(12℃熟成)と称した。
Next, after adding a predetermined amount of salt to the curd grain, 20% by weight of transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, packed and squeezed, and natural cheese (moisture 39.2) is added. % By weight, 47.1% by weight of milk fat, 1.45% by weight of salt, pH 5.39). As a transglutaminase preparation, KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used.
Transglutaminase was added in an amount of 0.002% by weight as a curd grain ratio. At the same time, a natural cheese to which no transglutaminase was added was produced in the same manner as described above.
These natural cheeses were ripened at 12 ° C., and the heat-retaining shape was measured in the same manner as in Test Example 1 for 1 month, 2 months, 3 months, and 4 months. Here, the system to which transglutaminase was added was referred to as Example 5 (aged at 12 ° C.), and the system to which no transglutaminase was added was referred to as Comparative Example 3 (aged at 12 ° C.).
[耐熱保形性の評価]
 表3~4の通り、熟成後に耐熱保形性を測定した結果、トランスグルタミナーゼを0.002%添加した実施例5は熟成1ヶ月後から耐熱保形性を有していた。一方、トランスグルタミナーゼを添加しない比較例3は耐熱保形性を有していなかった。
[Evaluation of heat-resistant shape retention]
As shown in Tables 3 to 4, as a result of measuring the heat-retaining shape after aging, Example 5 to which 0.002% of transglutaminase was added had heat-retaining shape after 1 month of aging. On the other hand, Comparative Example 3 in which no transglutaminase was added did not have heat-resistant shape retention.
Figure JPOXMLDOC01-appb-T000003
Figure JPOXMLDOC01-appb-T000003
Figure JPOXMLDOC01-appb-T000004
Figure JPOXMLDOC01-appb-T000004
[試験例3]
 製品ナチュラルチーズの固形分中の脂肪濃度が51重量%となるように、原料乳を調製し、63℃、30分間で加熱殺菌した後に、33℃に冷却してから、塩化カルシウム(0.03重量%)を添加した。次に、これらの原料乳に、チーズ製造用の市販スターター(L.Lactis ダニスコ社製)を0.1%添加した。これらスターターを添加した後に、カーフレンネット(力価:15000ユニット)を0.003重量%で添加し、原料乳を凝固させた。この凝固物をカッティングしホエイのpHが6.1~6.2となるまで攪拌して、ホエイを排出しカード粒を得た。
[Test Example 3]
The raw milk is prepared so that the fat concentration in the solid content of the product natural cheese is 51% by weight, sterilized by heating at 63 ° C. for 30 minutes, cooled to 33 ° C., and then calcium chloride (0.03 % By weight) was added. Next, 0.1% of a commercial starter for cheese manufacture (manufactured by L. Lactis Danisco) was added to these raw milks. After adding these starters, calf rennet (titer: 15000 units) was added at 0.003% by weight to coagulate the raw milk. The coagulated product was cut and stirred until the whey had a pH of 6.1 to 6.2, and the whey was discharged to obtain curd grains.
 次に、このカード粒に食塩を所定量で添加してから、20重量%のトランスグルタミナーゼ製剤水溶液をカード粒比1重量%で添加し、型詰めして圧搾し、ナチュラルチーズ(水分41.9重量%、乳脂肪分51.7重量%、塩分1.3重量%、pH5.27)を製造した。トランスグルタミナーゼ製剤としてはKS-CT(味の素(株)製:トランスグルタミナーゼ濃度1%、トランスグルタミナーゼ活性86~130U/g)を使用した。トランスグルタミナーゼは、カード粒比で0.002重量%添加した。また、同時にトランスグルタミナーゼを添加しないナチュラルチーズを上記と同様の方法で試作した。
 これらのナチュラルチーズを12℃で熟成し、熟成1ヶ月間、2ヶ月間、3ヶ月間、4ヶ月間における耐熱保形性を試験例1と同様の方法で測定した。ここで、トランスグルタミナーゼを添加した系を実施例6(12℃熟成)と称し、トランスグルタミナーゼを添加しない系を比較例4(12℃熟成)と称した。
Next, after adding a predetermined amount of salt to this curd grain, 20% by weight of a transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, packed and squeezed, and natural cheese (water content 41.9) is added. % By weight, milk fat content 51.7% by weight, salt content 1.3% by weight, pH 5.27). As a transglutaminase preparation, KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used. Transglutaminase was added in an amount of 0.002% by weight as a curd grain ratio. At the same time, a natural cheese to which no transglutaminase was added was produced in the same manner as described above.
These natural cheeses were ripened at 12 ° C., and the heat-retaining shape was measured in the same manner as in Test Example 1 for 1 month, 2 months, 3 months, and 4 months. Here, the system to which transglutaminase was added was referred to as Example 6 (aged at 12 ° C.), and the system to which no transglutaminase was added was referred to as Comparative Example 4 (aged at 12 ° C.).
[耐熱保形性の評価]
 表5~6の通り、熟成後に耐熱保形性を測定した結果、トランスグルタミナーゼを0.002重量%添加した実施例6は熟成1ヶ月後から耐熱保形性を有していた。一方、トランスグルタミナーゼを添加しない比較例4は耐熱保形性を有していなかった。
[Evaluation of heat-resistant shape retention]
As shown in Tables 5 to 6, as a result of measuring the heat retaining shape after aging, Example 6 to which 0.002% by weight of transglutaminase was added had heat retaining shape after one month of aging. On the other hand, Comparative Example 4 to which no transglutaminase was added did not have heat-resistant shape retention.
Figure JPOXMLDOC01-appb-T000005
Figure JPOXMLDOC01-appb-T000005
Figure JPOXMLDOC01-appb-T000006
Figure JPOXMLDOC01-appb-T000006
[試験例4]
 製品ナチュラルチーズの固形分中の脂肪濃度が2.8重量%となるように、原料乳を調製し、63℃、30分間で加熱殺菌した後に、33℃に冷却してから、塩化カルシウム(0.03重量%)を添加した。次に、これらの原料乳に、チーズ製造用の市販スターター(L. Lactis ダニスコ社製)を0.1%g添加した。これらスターターを添加した後に、カーフレンネット(力価:15000ユニット)を0.003重量%で添加し、原料乳を凝固させた。この凝固物をカッティングしホエイのpHが6.1~6.2となるまで攪拌して、ホエイを排出しカード粒を得た。
 次に、このカード粒に食塩を所定量で添加してから、20重量%のトランスグルタミナーゼ製剤水溶液をカード粒比1重量%で添加し、型詰めして圧搾し、ナチュラルチーズ(水分48.8重量%、乳脂肪分2.81重量%、塩分1.33重量%、pH5.28)を製造した。トランスグルタミナーゼ製剤としてはKS-CT(味の素(株)製:トランスグルタミナーゼ濃度1%、トランスグルタミナーゼ活性86~130U/g)を使用した。
[Test Example 4]
The raw material milk is prepared so that the fat concentration in the solid content of the product natural cheese is 2.8% by weight, sterilized by heating at 63 ° C. for 30 minutes, cooled to 33 ° C., and then calcium chloride (0 0.03 wt.%) Was added. Next, 0.1% g of a commercial starter for cheese manufacture (manufactured by L. Lactis Danisco) was added to these raw milks. After adding these starters, calf rennet (titer: 15000 units) was added at 0.003% by weight to coagulate the raw milk. The coagulated product was cut and stirred until the whey had a pH of 6.1 to 6.2, and the whey was discharged to obtain curd grains.
Next, after adding a predetermined amount of salt to the curd grain, 20% by weight of transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, packed and squeezed, and natural cheese (moisture 48.8) is added. % By weight, milk fat content 2.81% by weight, salt content 1.33% by weight, pH 5.28). As a transglutaminase preparation, KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used.
 トランスグルタミナーゼは、カード粒比で0.002重量%添加した。また、同時にトランスグルタミナーゼを添加しないナチュラルチーズを上記と同様の方法で試作した。
 これらのナチュラルチーズを12℃で熟成し、熟成1ヶ月間、2ヶ月間、3ヶ月間、4ヶ月間における耐熱保形性を試験例1と同様の方法で測定した。ここで、トランスグルタミナーゼを添加した系を実施例7(12℃熟成)と称し、トランスグルタミナーゼを添加しない系を比較例5(12℃熟成)と称した。
Transglutaminase was added in an amount of 0.002% by weight as a curd grain ratio. At the same time, a natural cheese to which no transglutaminase was added was produced in the same manner as described above.
These natural cheeses were ripened at 12 ° C., and the heat-retaining shape was measured in the same manner as in Test Example 1 for 1 month, 2 months, 3 months, and 4 months. Here, the system to which transglutaminase was added was referred to as Example 7 (12 ° C. aging), and the system to which no transglutaminase was added was referred to as Comparative Example 5 (12 ° C. aging).
[耐熱保形性の評価]
 表7~8の通り、熟成後に耐熱保形性を測定した結果、トランスグルタミナーゼを0.002%添加した実施例7は熟成1ヶ月後から耐熱保形性を有していた。一方、トランスグルタミナーゼを添加しない比較例5は、実施例7と比較して耐熱保形性を有していなかった。
[Evaluation of heat-resistant shape retention]
As shown in Tables 7 to 8, as a result of measuring the heat retaining shape after aging, Example 7 to which 0.002% of transglutaminase was added had heat retaining shape after 1 month of aging. On the other hand, Comparative Example 5 in which no transglutaminase was added did not have heat-resistant shape retention as compared with Example 7.
Figure JPOXMLDOC01-appb-T000007
Figure JPOXMLDOC01-appb-T000007
Figure JPOXMLDOC01-appb-T000008
Figure JPOXMLDOC01-appb-T000008
[試験例5]
 製品ナチュラルチーズの固形分中の脂肪濃度が40重量%となるように、原料乳を調製し、63℃、30分間で加熱殺菌した後に、33℃に冷却してから、塩化カルシウム(0.03重量%)を添加した。次に、これらの原料乳に、チーズ製造用の市販スターターを添加した。これらスターターを添加した後に、カーフレンネット(力価:15000ユニット)を0.003重量%で添加し、原料乳を凝固させた。この凝固物をカッティングしホエイのpHが6.1~6.2となるまで攪拌して、ホエイを排出しカード粒を得た。
[Test Example 5]
The raw milk is prepared so that the fat concentration in the solid content of the product natural cheese is 40% by weight, sterilized by heating at 63 ° C. for 30 minutes, cooled to 33 ° C., and then calcium chloride (0.03 % By weight) was added. Next, a commercial starter for cheese manufacture was added to these raw milks. After adding these starters, calf rennet (titer: 15000 units) was added at 0.003% by weight to coagulate the raw milk. The coagulated product was cut and stirred until the whey had a pH of 6.1 to 6.2, and the whey was discharged to obtain curd grains.
 次に、このカード粒に食塩を所定量で添加してから、20重量%のトランスグルタミナーゼ製剤水溶液をカード粒比1重量%で添加し、型詰めして圧搾し、ナチュラルチーズ(水分45.5重量%、乳脂肪分40.0重量%、塩分1.43重量%、pH5.30)を製造した。トランスグルタミナーゼ製剤としてはKS-CT(味の素(株)製:トランスグルタミナーゼ濃度1%、トランスグルタミナーゼ活性86~130U/g)を使用した。
 トランスグルタミナーゼは、カード粒比で0.002重量%添加した。また、同時にトランスグルタミナーゼを添加しないナチュラルチーズを上記と同様の方法で試作した。これらのナチュラルチーズを12℃で熟成し、熟成1ヶ月間、2ヶ月間、3ヶ月間、4ヶ月間における耐熱保形性を試験例1と同様の方法で測定した。ここで、トランスグルタミナーゼを添加した系を実施例8(12℃熟成)と称し、トランスグルタミナーゼを添加しない系を比較例6(12℃熟成)と称した。
Next, after adding a predetermined amount of salt to this curd grain, 20% by weight of transglutaminase preparation aqueous solution is added at a curd grain ratio of 1% by weight, stuffed and squeezed, and natural cheese (water content 45.5%) is added. % By weight, milk fat content 40.0% by weight, salt content 1.43% by weight, pH 5.30). As a transglutaminase preparation, KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used.
Transglutaminase was added in an amount of 0.002% by weight as a curd grain ratio. At the same time, a natural cheese to which no transglutaminase was added was produced in the same manner as described above. These natural cheeses were ripened at 12 ° C., and the heat-retaining shape was measured in the same manner as in Test Example 1 for 1 month, 2 months, 3 months, and 4 months. Here, the system to which transglutaminase was added was referred to as Example 8 (12 ° C. aging), and the system to which no transglutaminase was added was referred to as Comparative Example 6 (12 ° C. aging).
[耐熱保形性の評価]
 表9~10の通り、熟成後に耐熱保形性を測定した結果、トランスグルタミナーゼを0.002%添加した実施例8は熟成1ヶ月後から耐熱保形性を有していた。一方、トランスグルタミナーゼを添加しない比較例6は耐熱保形性を有していなかった。
[Evaluation of heat-resistant shape retention]
As shown in Tables 9 to 10, as a result of measuring the heat retaining shape after aging, Example 8 to which 0.002% of transglutaminase was added had heat retaining shape after 1 month of aging. On the other hand, Comparative Example 6 to which no transglutaminase was added did not have heat-resistant shape retention.
Figure JPOXMLDOC01-appb-T000009
Figure JPOXMLDOC01-appb-T000009
Figure JPOXMLDOC01-appb-T000010
Figure JPOXMLDOC01-appb-T000010
[試験例6]
比較例7:原料乳の凝乳中(ホエイ排出前)におけるトランスグルタミナーゼの添加
(乳酸発酵工程)
 原料乳11kg(脂肪率3.40%)を殺菌冷却後、31℃まで加温して、混合乳酸菌スターター(L. lactisおよびL. Cremoris、Chris. Hansen's Laboratories製)を2.25g添加し、60分間31℃に保持した。
(レンネッティング工程)
 乳酸発酵工程が35分間経過したところでアナトー色素0.72mlを加え、さらに5分後に塩化カルシウムを0.02%添加した。45分間の乳酸発酵工程終了後、カーフレンネット(single strength、Chris. Hansen's Laboratories製、力価14000ユニット)を2.25ml添加し、25~30分間静置させ、凝固物を得た。
[Test Example 6]
Comparative Example 7: Addition of transglutaminase during curdling of raw milk (before whey discharge) (lactic acid fermentation process)
After sterilizing and cooling 11 kg of raw milk (fat content 3.40%), the mixture was heated to 31 ° C., and 2.25 g of mixed lactic acid bacteria starter (manufactured by L. lactis and L. Cremoris, Chris. Hansen's Laboratories) was added. Hold at 31 ° C. for minutes.
(Rennetting process)
When the lactic acid fermentation process had passed for 35 minutes, 0.72 ml of Anato dye was added, and after 5 minutes, 0.02% of calcium chloride was added. After completion of the lactic acid fermentation process for 45 minutes, 2.25 ml of calf rennet (single strength, manufactured by Chris. Hansen's Laboratories, strength 14000 units) was added and allowed to stand for 25 to 30 minutes to obtain a coagulum.
(カッティング工程)
 得られた凝固物をカッティングし、さらにホエイを分離させた。
(クッキング工程)
 カッティング後5分間静置してから、静かに10分間攪拌し、引き続いて加温を開始した。クッキング工程では、まず31℃から33℃まで昇温させて15分間加温し、ここでトランスグルタミナーゼを添加した。トランスグルタミナーゼ製剤としてはKS-CT(味の素(株)製:トランスグルタミナーゼ濃度1%、トランスグルタミナーゼ活性86~130U/g)を使用した。トランスグルタミナーゼの添加量は原料とした乳中のタンパク質1g当たり10Uとした(10U/gpと表記)。次いで33℃から35℃まで15分間かけて昇温させ、さらに35℃から38℃に10分間加温した。クッキング工程を通じて、常にゆっくりとカード粒を壊さないように攪拌し続けた。
(Cutting process)
The obtained coagulum was cut and whey was further separated.
(Cooking process)
After cutting, the mixture was allowed to stand for 5 minutes, and then gently stirred for 10 minutes, and then heating was started. In the cooking step, the temperature was first raised from 31 ° C. to 33 ° C. and heated for 15 minutes, where transglutaminase was added. As a transglutaminase preparation, KS-CT (manufactured by Ajinomoto Co., Inc .: transglutaminase concentration 1%, transglutaminase activity 86-130 U / g) was used. The amount of transglutaminase added was 10 U per 1 g of protein in milk as a raw material (denoted as 10 U / gp). Next, the temperature was raised from 33 ° C. to 35 ° C. over 15 minutes, and further heated from 35 ° C. to 38 ° C. for 10 minutes. Throughout the cooking process, stirring was always continued slowly so as not to break the curd grains.
(チェダリング工程)
 次いで38℃で15分間、攪拌を続けて保持し、5~10分間静置してから、分離したホエイを排出させた。ホエイを排出させ、チーズカードを得たら、これを6インチ幅に切って積み重ねる。37℃~38℃に保持して、15分おきに反転を繰り返し(チェダリング)て、ホエイ排出を促した。
(ミリングおよび食塩添加工程)
 次に、チーズカードを砕くミリング操作を行う。砕いたチーズカードに食塩を徐々に混合し、食塩濃度がカードの4.5%になるように3回にわけて食塩を加えた。
(型詰工程)
 常法に従って、型詰めし圧搾した。
(Cedaring process)
Next, stirring was continued for 15 minutes at 38 ° C., and the mixture was allowed to stand for 5 to 10 minutes, and then the separated whey was discharged. When the whey is discharged and the cheese curd is obtained, it is cut into 6 inches and stacked. The temperature was maintained at 37 ° C. to 38 ° C., and inversion was repeated every 15 minutes (cheddaring) to promote whey discharge.
(Milling and salt addition process)
Next, a milling operation for breaking the cheese curd is performed. Salt was gradually mixed into the crushed cheese curd, and the salt was added in three portions so that the salt concentration was 4.5% of the curd.
(Mold filling process)
According to a conventional method, it was packed and pressed.
比較例8:原料乳の凝乳前(レンネッティング工程)におけるトランスグルタミナーゼの添加
 原料乳の凝乳中(ホエイ排出前)のトランスグルタミナーゼの添加の代わりに、トランスグルタミナーゼの添加をレンネットの添加と同時に行った以外は、を比較例7と同様の工程で、チェダーチーズを製造した。
Comparative Example 8: Addition of transglutaminase before curding raw material milk (rennetting process) Instead of adding transglutaminase during curdling raw material milk (before whey discharge), adding transglutaminase is the addition of rennet. Cheddar cheese was manufactured in the same process as in Comparative Example 7 except that it was performed simultaneously.
実施例9:原料乳の凝乳後(ミリング・食塩添加工程)でのトランスグルタミナーゼの添加
 原料乳の凝乳中(ホエイ排出前)のトランスグルタミナーゼの添加の代わりに、ホエイ排出後の砕いたチーズカードにトランスグルタミナーゼの添加をする以外は、比較例7と同様の工程で、チェダーチーズを製造した。トランスグルタミナーゼの添加量は、カード粒比で0.02重量%であった。
Example 9: Addition of transglutaminase after curding raw milk (milling and salt addition step) Crushed cheese after discharging whey instead of adding transglutaminase during curding raw material (before discharging whey) Cheddar cheese was produced in the same process as Comparative Example 7 except that transglutaminase was added to the curd. The amount of transglutaminase added was 0.02% by weight in terms of curd grain ratio.
[耐熱保形性の評価]
 これらのチェダーチーズを、12℃で熟成し、熟成1ヶ月間、2ヶ月間、3ヶ月間、4ヶ月間における耐熱保形性を試験例1と同様の方法で測定した。結果を表11および表12に示す。
[Evaluation of heat-resistant shape retention]
These cheddar cheeses were ripened at 12 ° C., and the heat-resistant shape retention was measured in the same manner as in Test Example 1 for 1 month, 2 months, 3 months, and 4 months. The results are shown in Table 11 and Table 12.
Figure JPOXMLDOC01-appb-T000011
Figure JPOXMLDOC01-appb-T000011
Figure JPOXMLDOC01-appb-T000012
Figure JPOXMLDOC01-appb-T000012
 表11および表12の通り、熟成後に耐熱保形性を測定した結果、トランスグルタミナーゼを凝乳・ホエイ排出後のチーズカードに添加した実施例9は熟成1ヶ月後から耐熱保形性を有していた。一方、トランスグルタミナーゼを凝乳中に添加した比較例7およびトランスグルタミナーゼを凝乳前、レンネットの添加と同時に添加した比較例8は耐熱保形性を有していなかった。また、比較例7および比較例8は熟成の過程で離水が発生した。 As shown in Tables 11 and 12, as a result of measuring the heat-resistant shape retention after aging, Example 9 in which transglutaminase was added to the cheese curd after curdling and whey discharge had heat-resistant shape retention after 1 month of aging. It was. On the other hand, Comparative Example 7 in which transglutaminase was added to the curd and Comparative Example 8 in which transglutaminase was added simultaneously with the addition of rennet did not have heat-resistant shape retention. In Comparative Examples 7 and 8, water separation occurred during the aging process.
[官能評価]
 比較例7、比較例8、実施例9のナチュラルチーズを熟成期間1ヶ月~6ヶ月の各時点における、目視での評価、および熟成風味の評価を表13に示す。
 比較例7および比較例8は熟成の過程で離水が発生した。
 熟成風味については、いずれも12℃での熟成を3カ月以上行うことにより強い熟成風味を有するナチュラルチーズとなった。
[sensory evaluation]
Table 13 shows the visual evaluation and the evaluation of the ripening flavor of the natural cheeses of Comparative Example 7, Comparative Example 8, and Example 9 at each time point of the ripening period of 1 month to 6 months.
In Comparative Example 7 and Comparative Example 8, water separation occurred during the aging process.
As for the ripening flavor, ripening at 12 ° C. for 3 months or more resulted in a natural cheese having a strong ripening flavor.
Figure JPOXMLDOC01-appb-T000013
Figure JPOXMLDOC01-appb-T000013
 以上の結果から、トランスグルタミナーゼをホエイの排出後に添加し、熟成させることにより、耐熱保形性および良好な熟成風味を有する熟成型ナチュラルチーズが得られることが理解できる。 From the above results, it can be understood that by adding transglutaminase after the whey is discharged and ripening, a ripened natural cheese having heat-resistant shape retention and good ripening flavor can be obtained.
 本発明の耐熱保形性を有するナチュラルチーズおよびその製造方法において、原料乳の水分および乳タンパク質の組成を調整することなく、耐熱保形性を有するナチュラルチーズおよびその製造方法を見出した。このナチュラルチーズは、フライパンやオーブンなどによる加熱などの調理・加工をした時にチーズが溶融や変形をすることなく、良好な耐熱保形性を有する新規のナチュラルチーズおよびその製造方法を提供できる。 In the natural cheese having heat-resistant shape retention of the present invention and a method for producing the same, the present inventors have found a natural cheese having heat-resistant shape-retaining properties and a method for producing the same without adjusting the composition of the moisture and milk protein of the raw milk. This natural cheese can provide a novel natural cheese having a good heat-resistant shape retaining property and a method for producing the same without causing the cheese to melt or deform when cooking or processing such as heating in a frying pan or an oven.

Claims (11)

  1.  原料乳を凝乳させた後にホエイを排出してチーズカードを調製し、当該チーズカードにトランスグルタミナーゼを添加して熟成させることを含む、耐熱保形性を有するナチュラルチーズの製造方法。 A method for producing natural cheese having heat-resistant shape retention, comprising preparing curd by discharging whey after curdling raw milk, adding transglutaminase to the cheese curd and aging.
  2.  凝乳酵素と乳酸菌を添加して凝乳させる、請求項1に記載の製造方法。 The production method according to claim 1, wherein the milk-clotting enzyme and lactic acid bacteria are added to cause the milk to coagulate.
  3.  熟成させることが、4~20℃、1~4ヶ月間で熟成させることである、請求項1または2に記載の製造方法。 The production method according to claim 1 or 2, wherein the aging is aging at 4 to 20 ° C for 1 to 4 months.
  4.  トランスグルタミナーゼを添加した後に、トランスグルタミナーゼが失活する温度および時間で加熱することを含まない、請求項1~3のいずれか一項に記載の製造方法。 The production method according to any one of claims 1 to 3, which does not include heating at a temperature and a time at which the transglutaminase is deactivated after the transglutaminase is added.
  5.  トランスグルタミナーゼを添加した後かつ熟成する前に、トランスグルタミナーゼが失活する温度および時間で加熱することを含まない、請求項1~3のいずれか一項に記載の製造方法。 The production method according to any one of claims 1 to 3, which does not include heating at a temperature and a time at which the transglutaminase is inactivated after the transglutaminase is added and before ripening.
  6.  請求項1~5のいずれか一項に記載の製造方法により製造された、耐熱保形性を有するナチュラルチーズ。 Natural cheese having heat-resistant shape retention produced by the production method according to any one of claims 1 to 5.
  7.  150~250℃、5~20分間の乾熱下の焼成および/または100~200℃、5~40分間の湿熱下の焼成において保形性を有する、請求項6に記載のナチュラルチーズ。 The natural cheese according to claim 6, which has shape retention in baking under dry heat at 150 to 250 ° C for 5 to 20 minutes and / or baking under wet heat at 100 to 200 ° C for 5 to 40 minutes.
  8.  150~250℃、5~20分間の乾熱下の焼成において50%以上の保形性、および/または100~200℃、5~40分間の湿熱下の焼成において40%以上の保形性を有する、請求項6または7に記載のナチュラルチーズ。 50% or more of shape retention in baking at 150 to 250 ° C. for 5 to 20 minutes, and / or 40% or more in shape retention at 100 to 200 ° C. for 5 to 40 minutes in wet heat The natural cheese of Claim 6 or 7 which has.
  9.  請求項6~8のいずれか一項に記載のナチュラルチーズを含む、加熱調理食品。 Cooked food containing the natural cheese according to any one of claims 6 to 8.
  10.  請求項6~8のいずれか一項に記載のナチュラルチーズを内包して含む、加熱調理食品。 A cooked food containing the natural cheese according to any one of claims 6 to 8.
  11.  加熱調理食品用である、請求項6~8のいずれか一項に記載のナチュラルチーズ。 The natural cheese according to any one of claims 6 to 8, which is used for cooked food.
PCT/JP2015/076105 2014-09-16 2015-09-15 Natural cheese exhibiting heat-resistant shape retention and method for manufacturing said cheese WO2016043177A1 (en)

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JPWO2018181696A1 (en) * 2017-03-30 2020-02-06 日本ゼオン株式会社 Optical film, manufacturing method thereof, polarizing plate, and liquid crystal display device
WO2022173027A1 (en) * 2021-02-15 2022-08-18 味の素株式会社 Methods respectively for producing cheese and cheese analogue using enzyme

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* Cited by examiner, † Cited by third party
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US20020106423A1 (en) * 2001-01-25 2002-08-08 Nutricepts, Inc. Shaped cheese reconstruction with transglutaminase
US20030165594A1 (en) * 2002-03-04 2003-09-04 Schreiber Foods, Inc. Processed cheese with improved firmness using cross-linking enzymes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020106423A1 (en) * 2001-01-25 2002-08-08 Nutricepts, Inc. Shaped cheese reconstruction with transglutaminase
US20030165594A1 (en) * 2002-03-04 2003-09-04 Schreiber Foods, Inc. Processed cheese with improved firmness using cross-linking enzymes

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2018181696A1 (en) * 2017-03-30 2020-02-06 日本ゼオン株式会社 Optical film, manufacturing method thereof, polarizing plate, and liquid crystal display device
WO2022173027A1 (en) * 2021-02-15 2022-08-18 味の素株式会社 Methods respectively for producing cheese and cheese analogue using enzyme

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