WO2016026829A9 - Method of extracting nucleic acids - Google Patents

Method of extracting nucleic acids Download PDF

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Publication number
WO2016026829A9
WO2016026829A9 PCT/EP2015/068887 EP2015068887W WO2016026829A9 WO 2016026829 A9 WO2016026829 A9 WO 2016026829A9 EP 2015068887 W EP2015068887 W EP 2015068887W WO 2016026829 A9 WO2016026829 A9 WO 2016026829A9
Authority
WO
WIPO (PCT)
Prior art keywords
microwave
sample
nucleic acids
electromagnetic radiation
vessel
Prior art date
Application number
PCT/EP2015/068887
Other languages
French (fr)
Other versions
WO2016026829A1 (en
Inventor
Wolfgang Weber
Christine Werner
Nadine NOAK
Original Assignee
Ifp Privates Institut Fuer Produktqualitaet Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ifp Privates Institut Fuer Produktqualitaet Gmbh filed Critical Ifp Privates Institut Fuer Produktqualitaet Gmbh
Priority to EP15760101.4A priority Critical patent/EP3183344A1/en
Publication of WO2016026829A1 publication Critical patent/WO2016026829A1/en
Publication of WO2016026829A9 publication Critical patent/WO2016026829A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The method of isolating nucleic acids from a sample for further analysis by a DNA polymerase chain reaction, wherein the cells of the sample are lysed in aqueous solution using dielectric heating, which encompasses subjecting the sample to microwave electromagnetic radiation having wavelengths ranging from 11.8 to 12.6 cm, comprises the steps of providing a vessel made of microwave-transparent material with closable means and loading said vessel with a sample to be analyzed, a tablet comprising water insoluble hydrated magnesium silicate and crystalline phosphate buffer saline, and water; selecting a hollow receptacle made of microwave-reflecting material which cooperates with microwave electromagnetic radiation; placing one or more closed vessels containing said loaded mixture inside said hollow receptacle; introducing one or more said receptacles with one or more said closed vessels inside a microwave oven; and microwaving, so that the microwave electromagnetic radiation elicits a temperature rise of the mixture inside the closed vessel up to 95°C, and nucleic acids are released and a solution of nucleic acids suitable for PCR analysis is provided.
PCT/EP2015/068887 2014-08-18 2015-08-18 Method of extracting nucleic acids WO2016026829A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP15760101.4A EP3183344A1 (en) 2014-08-18 2015-08-18 Method of extracting nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102014111738.7 2014-08-18
DE102014111738 2014-08-18

Publications (2)

Publication Number Publication Date
WO2016026829A1 WO2016026829A1 (en) 2016-02-25
WO2016026829A9 true WO2016026829A9 (en) 2016-08-25

Family

ID=54065321

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2015/068887 WO2016026829A1 (en) 2014-08-18 2015-08-18 Method of extracting nucleic acids

Country Status (2)

Country Link
EP (1) EP3183344A1 (en)
WO (1) WO2016026829A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201619530D0 (en) * 2016-11-18 2017-01-04 Univ College Cardiff Consultants Ltd The preparation of samples for constituent analysis

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2868082B1 (en) * 2004-03-26 2006-06-09 Amenagement Urbain & Rural PROCESS FOR EXTRACTING INTRACELLULAR COMPOUNDS OR COMPLEXES FROM A SAMPLE CONTAINING CELLS IN SUSPENSION IN A LIQUID
KR101380909B1 (en) * 2013-03-20 2014-04-01 한국생명공학연구원 Absorbents for purification of nucleic acid and a purification method using the absorbents

Also Published As

Publication number Publication date
WO2016026829A1 (en) 2016-02-25
EP3183344A1 (en) 2017-06-28

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