WO2016017811A1

WO2016017811A1 - Selective biomaterial adsorption/desorption material

Info

Publication number: WO2016017811A1
Application number: PCT/JP2015/071853
Authority: WO
Inventors: 森　寛; 勝下山; 隆伸香月
Original assignee: 日本化成株式会社
Priority date: 2014-08-01
Filing date: 2015-07-31
Publication date: 2016-02-04
Also published as: JPWO2016017811A1; JP6658526B2

Abstract

This invention addresses the problem of providing a selective biomaterial adsorption/desorption material for isolating a biomaterial recovery target from a bodily fluid or the like via selective adsorption/desorption; specifically, a selective biomaterial adsorption/desorption material that is easy to handle, makes automation using an analysis device easy, and makes it possible to isolate and recover a biomaterial recovery target quickly, efficiently, and with a high recovery rate. Said problem is solved by a selective biomaterial adsorption/desorption material for isolating a biomaterial recovery target from a biomaterial-containing liquid via selective absorption/desorption, said selective biomaterial adsorption/desorption material being characterized by being a sheet that contains a porous body that has mesopores.

Description

Selective absorption / desorption materials for biological materials

TECHNICAL FIELD The present invention relates to a biological material selective absorption / desorption material for selectively absorbing / desorbing a biological material to be collected from a biological material-containing liquid, and in particular, a peptide from a biological fluid such as blood. The present invention relates to a biological material selective absorption / desorption material for efficiently separating and recovering biological material.
The present invention also relates to a method for producing a high-purity peptide and a method for separating a peptide using the biological material selective adsorption / desorption material.

In recent years, peptides in blood (serum or plasma) have attracted attention as biomarkers for early detection and diagnosis of cancer, hepatitis, and other diseases. Since peptides specific for diseases exist in peptides of disease patients, early detection / diagnosis of diseases such as cancer becomes possible by detecting these peptides.

In order to analyze peptides as biomarkers, it is necessary to selectively separate and collect peptides from blood collected from diseased patients. Conventionally, as a method for separating and recovering peptides from blood, silica having mesopores (referred to as “mesoporous silica” in the present invention) by utilizing the property that peptides selectively enter and adsorb into mesopores of silica. .) Has been proposed as a peptide adsorption / desorption material.

For example, Patent Document 1 proposes a method in which a peptide contained in serum or plasma is mixed with a dry powder of mesoporous silica having a honeycomb structure so that the peptide is adsorbed and recovered in mesopores of mesoporous silica. ing. Specifically, first, a mesoporous silica powder is adsorbed in a mesopore of mesoporous silica by stirring the dry powder of mesoporous silica in a liquid sample containing the peptide, and then the peptide is adsorbed by centrifugation. Silica is recovered. The recovered product is washed with water and then treated with an acidic buffer to peel and remove proteins or long-chain peptides adsorbed on the outer surface of the mesoporous silica particles. Next, after washing with water, the peptide (short chain peptide) adsorbed in the mesopores of mesoporous silica is recovered by desorption and elution by treating with a peptide recovery solvent consisting of an aqueous solution containing acetonitrile, and this is analyzed. .
In the method of Patent Document 1, since a dry powder of mesoporous silica is used, centrifugation is performed between the steps of mixing mesoporous silica and blood, washing with water, treatment with an acidic buffer, and treatment with a peptide recovery solvent. Requires a solid-liquid separation process of mesoporous silica powder.

International Publication WO2011 / 062270 Pamphlet

The method for separating and recovering peptides using mesoporous silica is an effective method for selectively separating and recovering peptides from blood, but in the future for clinical application, it will be used for automation by analyzers. Further improvements are required.

That is, in the conventional method, mesoporous silica is used as a dry powder, and this is put into blood as it is and stirred. Thus, including the step of mixing blood and mesoporous silica, washing with water, treatment with an acidic buffer, peptide In each step of treatment with the recovered solvent, it is necessary to perform solid-liquid separation operations by stirring and mixing the powder and treatment liquid and centrifuging multiple times, and analyze the method that requires such complicated operations multiple times. It is difficult to automate using an apparatus.

In addition, mesoporous silica powder has the following drawbacks.
(1) Even if mesoporous silica powder is put into the liquid and stirred, sufficient contact efficiency cannot be obtained, and the peptide adsorption efficiency and desorption efficiency are low, so that the treatment takes a long time.
(2) Originally, it is difficult to thoroughly wash highly hydrophilic silica in a powder state and to separate into solid and liquid by centrifugation, because of residual components contained in the powder suspension. The analysis results may be affected.
(3) The recovery efficiency of mesoporous silica powder by centrifugation is poor, and the recovery loss of peptides is large. The low peptide recovery rate is also a cause of insufficient analysis accuracy.
(4) Unrecovered powder becomes a clogging substance or a pollutant, which hinders stable operation of the device.

The present invention solves the above-mentioned conventional problems, is a selective absorption / desorption material of biological material for selectively absorbing / desorbing and separating the biological material to be collected from the liquid containing biological material, This is a material for selective absorption and desorption of biological materials that is easy to handle, easy to be automated by an analyzer, and capable of separating and collecting biological materials to be collected efficiently in a short time and with a high recovery rate. It is an object of the present invention to provide a method for producing a high-purity peptide and a method for separating a peptide using a material for selectively absorbing and desorbing a biological substance.

As a result of intensive studies to solve the above problems, the present inventors have found that the above problems can be solved by using a sheet including a porous body having mesopores as an absorbent / desorbing material, The present invention has been completed.

That is, the gist of the present invention is as follows.
[1] A selective absorption / desorption material for a biological material for selectively absorbing / desorbing a biological material from a liquid containing the biological material, the absorption / desorption material having mesopores A selective absorption / desorption material for a biological material, which is a sheet containing a porous body.
[2] The biological material according to [1], wherein the absorption / desorption material is a laminate in which an absorption / desorption layer including a porous body having mesopores and a water-permeable layer are laminated. Selective absorption / desorption material.
[3] The biological material according to [2], wherein the absorption / desorption material is a laminate in which a water-permeable layer is laminated on both surfaces of an absorption / desorption layer including a porous body having mesopores. Selective suction and desorption material.
[4] The selective adsorption / desorption material for biological material according to [2], wherein the adsorption / desorption material has a porous material having mesopores and a layer containing a binder.
[5] A biological material selective absorption / desorption material for selectively absorbing / desorbing a biological material from a liquid containing the biological material, the absorption / desorption material having mesopores A selective absorption / desorption material for biological materials, characterized in that it is a laminate in which an absorption / desorption layer containing a porous body and a water-permeable layer are laminated.
[6] A selective absorption / desorption material for a biological material for selectively absorbing / desorbing a biological material from a liquid containing the biological material, the absorption / desorption material having mesopores A selective absorption / desorption material for biological materials, comprising a layer containing a porous body and a binder.
[7] The biological material selective adsorption / desorption material according to any one of [1] to [6], wherein the porous body having mesopores has a mesopore diameter of 2 nm to 20 nm.
[8] The selective adsorption / desorption material for biological material according to any one of [1] to [7], wherein the porous body having mesopores has an average particle diameter of 50 μm or more and 700 μm or less.
[9] The selective absorption / desorption material for a biological material according to any one of [1] to [8], wherein the porous body having mesopores has a specific surface area of 100 m ² / g or more and 1200 m ² / g or less. .
[10] The biological material according to any one of [1] to [9], wherein the porous body having mesopores includes one or more selected from the group consisting of mesoporous silica, silica gel, and ion exchange resin. Selective absorption / desorption material.
[11] The material for selectively absorbing and desorbing a biological material according to any one of [2] to [5] and [7] to [10], wherein the water-permeable layer is a woven fabric or a nonwoven fabric.
[12] The selective absorption / desorption material for a biological material according to any one of [2] to [11], wherein the water-permeable layer has a hydrophilic treatment on an outer surface.
[13] The selective adsorption / desorption material for a biological material according to any one of [2] to [12], wherein the water-permeable layer has a hydrophobizing treatment on an outer surface.
[14] The selective adsorption / desorption material for biological material according to any one of [1] to [13], wherein the biological material is a peptide.
[15] The selective adsorption / desorption material for a biological material according to any one of [4] and [6] to [14], wherein the binder is an ethylene vinyl acetate copolymer.
[16] The binder according to any one of [4] and [6] to [15], wherein the binder is contained in a layer containing a porous body having mesopores and a binder in an amount of 20 wt% to 80 wt%. Selective absorption / desorption material for biological materials.
[17] A container capable of holding a liquid containing the biological material, the container having the absorption / desorption material according to any one of [1] to [16] inside.
[18] A biological material purification kit comprising the absorption / desorption material according to any one of [1] to [16] and a container capable of holding a liquid containing the biological material.
[19] A method for producing a biological material, comprising the step of bringing the selective adsorption / desorption material for biological material according to any one of [1] to [16] into contact with a liquid containing the biological material.
[20] A method for separating a biological material, comprising the step of bringing the selective absorbing / desorbing material for a biological material according to any one of [1] to [16] into contact with a liquid containing the biological material.

The selective absorption / desorption material for biological materials of the present invention has the following effects.

(1) Since it is a sheet-like (layered) molded body, solid-liquid separation operation such as centrifugation is unnecessary. For example, the biological material to be collected can be recovered simply by immersing it in a liquid containing biological material and pulling it up. It can be adsorbed to a porous body having mesopores in the adsorption / desorption layer. In addition, since washing with water and treatments with various treatment liquids can be performed with simple operations, automation by an analyzer is easy.
(2) The contact efficiency with a liquid containing a biological substance is high, and the contact efficiency with these liquids is high in the case of treatment with a treatment liquid such as water washing, an acidic buffer solution, and a peptide recovery solvent. Therefore, the adsorption efficiency and desorption efficiency of the biological material are high, and the biological material can be stably separated and recovered with a high yield. Further, the adsorption speed, desorption speed, washing efficiency, and processing efficiency are high, and the time required for the operation of sucking and desorbing the biological material can be greatly shortened. Furthermore, since the washing efficiency and the processing efficiency are high, it is possible to recover a high-purity biological material with few undesirable residual components, and it is possible to perform an accurate analysis without being affected by impurities.
(3) By designing the porous body having mesopores so that it does not fall off, as in the case of using a porous body having mesopores, the unrecovered powder becomes a clogging substance or a pollutant so that the apparatus can be operated stably. The problem of obstruction can be solved.

The embodiment of the selective absorption / desorption material for biological materials of the present invention is shown in (a) the first embodiment, (b) the second embodiment, (c) the schematic cross section of the third embodiment. FIG. It is a schematic diagram which shows an example of the shape of the selective absorption-desorption material of the biological material of this invention, Comprising: (a), (d), (e) figure is a perspective view, (b), (c) figure is a front view FIG. It is process explanatory drawing which shows an example of the manufacturing method of the selective absorption / desorption material of the biological material of this invention.

Hereinafter, embodiments of the present invention will be described in detail.
In the following, the biological material selective absorption / desorption material according to the embodiment of the present invention is brought into contact with a liquid containing the biological material, the treatment is brought into contact with water for cleaning, and the biological material other than the biological material to be collected "Wetted process" is a combination of the process of contacting with a treatment liquid to remove and remove unnecessary materials, the process of contacting with a desorption liquid such as a biological material recovery solvent for desorption of a biological material to be collected, etc. Sometimes called.
For example, when the biological material to be collected is a peptide (short chain peptide), unnecessary substances other than the biological material to be collected include proteins or long-chain peptides, etc., and the treatment liquid for peeling off unnecessary substances is acidic. Examples include a buffer solution.

[Selective absorption and desorption material for biological materials]
The biological material selective absorption / desorption material according to the present embodiment includes a form (hereinafter, also referred to as a first embodiment) which is a sheet including a porous body having mesopores.
Further, another embodiment of the adsorbing / desorbing material according to the present embodiment may be a laminate in which an adsorbing / desorbing material including a porous body having mesopores and a water-permeable layer are laminated (hereinafter referred to as the first). 2).
In addition, as another embodiment of the adsorbing / desorbing material according to the present embodiment, a layer including a porous body having mesopores and a binder may be included (hereinafter also referred to as a third embodiment).

The absorption / desorption material of the present invention is not particularly limited as long as it includes a porous body having mesopores and has a sheet-like structure. However, for example, a thrombotic material that becomes a blood coagulation factor is not preferable. The overall adsorbent / desorbable material is preferably made of a biocompatible material such as a bioinert material or an antithrombotic material.
However, even in the case of bioincompatible materials, those in which the contact surface with the biological fluid is made of a thrombotic material or a bioinert material by a surface treatment or the like is selected as a biocompatible material. It can be used as a constituent material of the absorption / desorption material.

In addition, since the adsorbing / desorbing material according to the first embodiment is desired to have the following characteristics or conditions, it is preferable to devise various processing and sheet design for that purpose.
(1) Since the porous material having mesopores selectively absorbs and desorbs biological materials, in order to increase the amount of collected biological materials, the porous materials having mesopores contained in the absorption / desorption material It is preferable that both the surface area and the surface area of the mesopores and the pore volume are large.
(2) It is preferable to increase the outer surface area of the porous body contained in the adsorption / desorption material in order to increase the efficiency of contact with the liquid during the liquid contact treatment. In order to increase the outer surface area of the porous body, for example, the particle diameter of the porous body may be reduced, or the absorption / desorption layer may be thinned.
(3) In order to increase the contact efficiency with the liquid during the liquid contact treatment, the porous body having mesopores preferably has good wettability with the liquid during the liquid contact treatment. Moreover, it is preferable that each member constituting the absorbent / desorbing material, for example, a material constituting the water-permeable layer is subjected to a hydrophilic treatment, or the specific gravity of the absorbent / desorbing material is adjusted so as to be easily immersed in the liquid.
(4) The porous body having mesopores contained in the absorption / desorption material may or may not flow during the liquid contact treatment. From the viewpoint of contact efficiency with the liquid, it is preferable that the fluid flows. If the flow is too intense, the particles may be damaged by the collision between the porous bodies having mesopores, and there is a possibility that "powdery" occurs where fine porous powder is generated and dropped. Therefore, the dropped porous powder becomes a clogging factor or a contamination factor of the apparatus, and the recovery rate of the peptide also deteriorates. Therefore, it is preferable to adjust the degree of flow to such an extent that no powder leaks. On the other hand, it is preferable that the porous body does not flow during the liquid contact treatment in order to suppress powder leakage and improve the handleability. In order not to flow, the porous body is preferably fixed with a binder.

In addition, the said description corresponding to the suction / desorption material which concerns on 2nd Embodiment and 3rd Embodiment can be used. Moreover, the description regarding the component requirements of the invention shown below can be applied not only to the first embodiment but also to the absorption / desorption materials according to the second embodiment and the third embodiment. The expression “embodiment of the present invention” includes the first embodiment, the second embodiment, and the third embodiment, and includes all other embodiments that the present invention can include.

<Liquids and biological substances containing biological substances>
In the embodiment of the present invention, the liquid containing the biological material to be processed typically includes blood (serum or plasma) which is a biological fluid, but is not limited thereto, for example, a biological fluid present in the living body. Alternatively, the liquid discharged from the living body to the outside and all of these processing liquids are included. The treatment liquid is a liquid obtained by treating a biological fluid, and the treatment method is not limited as long as the biological substance can be separated and recovered, and a known method can be applied. Further, the living body is not limited to the human body. Specific examples of biological fluids include, but are not limited to, the following.
Blood, lymph, tissue fluid (interstitial fluid, intercellular fluid, interstitial fluid), pleural effusion, ascites, serous fluid such as pericardial fluid, cerebrospinal fluid (spinal fluid), joint fluid (synovial fluid), aqueous humor ( Aqueous humor), saliva, gastric juice, bile, pancreatic juice, intestinal juice, etc., sweat, tears, runny nose, urine, semen, vaginal fluid, amniotic fluid, milk.

The biological material to be separated and recovered from the liquid containing the biological material is not particularly limited as long as it is selectively penetrated and adsorbed into the porous body having mesopores, but for early detection and diagnosis of diseases. Biological substances that can be used as biomarkers are preferably, for example, biomarkers derived from nucleic acids, peptides, proteins, lipid metabolites, carbohydrate metabolites, and the like. Examples of the nucleic acid include DNA and RNA.
The dynamic diameter of the biological material in the liquid is usually 0.3 nm or more, preferably 0.4 nm or more, more preferably 0.5 nm or more, and usually 15 nm or less, preferably 12 nm or less, more preferably 10 nm or less. It is. When the biological substance is a peptide, a short chain peptide having an amino acid residue length of 10 to 100, preferably 10 to 50 is particularly mentioned.
The liquid containing the biological material preferably has a small content of impurities other than the biological material to be separated and recovered, and the impurities can be removed by a known method. For example, when the biological substance is a peptide, it is preferable that the content of impurities such as proteins such as albumin and globulin is small.

<Porous body having mesopores>
The porous body having mesopores is not particularly limited as long as it has mesopores and is a porous body. Specific examples include mesoporous silica, silica gel, silica / alumina, ion exchange resin, diatomaceous earth, activated carbon, zeolite, and acid clay. Of these, mesoporous silica, silica gel, and ion exchange resin are preferable from the viewpoint of adsorption and desorption of biological substances.
The mesopore diameter is usually 2 nm or more, and usually 50 nm or less, preferably 20 nm or less, more preferably 10 nm or less, still more preferably 8 nm or less, and particularly preferably 5 nm or less.
The pore diameter of the mesopores is preferably set according to the type of biological material that is the target of separation and recovery. For example, in order to separate and recover peptides, it is usually 1 nm or more, preferably 2 nm or more, more preferably 3 nm or more, usually 15 nm or less, preferably 12 nm or less, more preferably 10 nm or less.

A porous body having mesopores may have pores such as macropores that are not included in the mesopores as long as it has mesopores. In order to separate and recover, the porous body having mesopores preferably has only mesopores.
The pore diameter of the porous body having mesopores was determined from EP Barrett, LG Joyner, PHHaklenda, J. Amer. Chem. Soc., Vol. 73, 373 (1951 Plot distribution curve calculated by the BJH method described in the above), that is, the differential nitrogen gas adsorption amount (ΔV / Δ (logd); V is the nitrogen gas adsorption volume) is plotted against the pore diameter d (nm). Although it can be obtained from the figure, catalog values can be adopted for commercial products.

Further, the pore volume per unit weight of the porous body having mesopores (in this specification, the amount represented by “pore volume / weight” is simply referred to as “pore volume”) is usually 0.00. 1 ml / g or more, preferably 0.2 ml / g or more, and usually 1.5 ml / g or less, preferably 1.2 ml / g or less. If the pore volume is less than the above lower limit, the adsorption / desorption performance tends to be inferior, and if the upper limit is exceeded, the pore structure and particles are easily broken by the liquid contact treatment, and the adsorption / desorption selectivity is reduced, or the powder leaks. May occur. The pore volume of the porous body having mesopores can be determined from the amount of nitrogen gas adsorbed at a relative pressure of 0.98 of the adsorption isotherm, but a catalog value can be adopted for a commercial product.

The shape of the porous body having mesopores is not particularly limited as long as the porous body can be formed into a sheet (layered) shape, and may be in the form of a crushed shape, a spherical shape, or a monolith or granulated particle. There may be. Moreover, the thing of the honeycomb structure described in patent document 1 may be sufficient. In the case of granulated particles, those having large gaps between primary particles are preferable in terms of contact efficiency with biological fluids and the like.

If the size of the porous body having mesopores is too small, the surface area outside the particle will increase with respect to the total surface area (the total surface area inside the mesopore and the surface area outside the particle). On the other hand, depending on the adsorbing substance, there is a risk that the adsorption selectivity may be lowered. Conversely, for large particles, the pore length in the particles is long. It takes time to penetrate and diffuse into the pores, and it takes a long time for absorption / desorption, washing, and collection of biological materials, which is not preferable.

The size of the porous body having mesopores has an optimum value according to the biological material to be collected and cannot be defined unconditionally. For example, it is 80% or more, preferably 90% or more, more preferably, of all particles. The maximum ferret diameter of 95% or more of the particles is preferably 20 μm or more, more preferably 50 μm or more, preferably 1 mm or less, more preferably 800 μm or less.
The average particle diameter is preferably 50 μm or more, more preferably 70 μm or more, preferably 700 μm or less, more preferably 600 μm or less.

Here, the maximum ferret diameter is the maximum value of the so-called directional tangent diameter, which corresponds to the diameter of spherical particles, and in the case of irregularly shaped particles such as crushed particles, the particles are divided into two parallel fixed directions. When sandwiched between tangent lines, this corresponds to the length of the part where the distance between the lines is the longest. The maximum ferret diameter can be determined, for example, by observing particles with an optical microscope and performing image analysis (hereinafter, the maximum ferret diameter may be referred to as “particle size”). The ratio of particles having a predetermined diameter in all particles can be determined by arbitrarily selecting 100 or more particles.
The average particle diameter is an average value of the particle sizes of the primary particles. The average particle size can be obtained from the particle size distribution measured by a laser diffraction / scattering type particle size distribution analyzer (for example, Laser Micron Sizer LMS-24 manufactured by Seishin Enterprise). Can be adopted.

The porous body having mesopores has a specific surface area of usually 100 m ² / g or more, preferably 200 m ² / g, and usually 1200 m ² / g or less, preferably 1000 m ² / g or less. When the specific surface area is at least the above lower limit, the target product can be efficiently absorbed and desorbed. By being below the above upper limit, the strength of the porous body can be ensured and the durability of the absorbent / desorbable material can be improved.
The specific surface area is measured by the BET single point method.

The porous body having mesopores may be used after being subjected to a surface treatment such as a hydrophobization treatment in order to prevent substances other than the target substance from adsorbing to the outer surface. As an example of surface treatment, the outer surface of a porous body having mesopores is hydrophobized by silicone treatment and trimethylsilylation treatment, and the target biological material is selectively contained only in the pores of the porous body having mesopores. You may modify | reform so that it may adsorb | suck to. In this case, if such a treatment is also performed in the pores of the porous body having mesopores, it becomes difficult for the target substance to be adsorbed in the pores, so that the surface treatment is performed only on the outer surface of the particles. Thus, it is important to adjust the degree of the processing.

<Suction / desorption layer>
The absorption / desorption layer may be composed only of a porous body having mesopores, or may be composed of a porous body having mesopores and another material.
As materials other than porous bodies having mesopores that may be contained in the absorption / desorption layer, the material having the mesopores does not significantly impair the absorption / desorption function of biological substances, and more There is no particular limitation as long as it is, but, for example, fixing a porous body having mesopores to improve the molding stability of the absorption / desorption layer, a binder for preventing the above-mentioned powder leakage, and adjusting the specific gravity Inorganic filler for the purpose, coarse particles of silica for improving the wettability.
The first embodiment of the present invention includes an aspect in which the material constituting the absorption / desorption layer flows during the liquid contact treatment. In this case, the absorption / desorption layer may or may not include a binder.

<Binder>
The binder is not particularly limited as long as a porous body having mesopores can be molded, but a thermoplastic resin is preferably used. The thermoplastic resin is not particularly limited as long as it can mold a porous body having mesopores (for example, adhesion by thermal fusion). Preferably, the water absorption and MFR described below are satisfied. For example, polyethylene, polypropylene, ethylene vinyl acetate copolymer, polyvinyl acetate, saponified ethylene vinyl acetate copolymer, polyvinyl alcohol, polyester, polyamide, Examples thereof include polyurethane, ionomer resin, and the like. From the viewpoint of moldability and solvent resistance of the porous body, ethylene vinyl acetate copolymer, saponified ethylene vinyl acetate copolymer, polyester, polyamide, and polyethylene are preferable. Among them, ethylene acetic acid has the advantage that the elution of the components is small, the absorption / desorption characteristics of the porous body having mesopores are difficult to deteriorate, and the porous body having mesopores is not easily dropped even if the absorption / desorption material is subjected to external stress. A vinyl copolymer is more preferable.

It is preferable that the thermoplastic resin has a water absorption rate of 0.2% or more measured by the following method. A thermoplastic resin having a water absorption rate of less than 0.2% is highly hydrophobic, and in the absorption / desorption layer, the thermoplastic resin exposed in the gaps between the mesoporous silica particles repels water, so that the absorption / desorption material Absorption / desorption performance may be inferior. The higher the water absorption rate of the thermoplastic resin, the more preferable, and particularly 0.5% or more is preferable. The upper limit of the water absorption rate of the thermoplastic resin is usually 10% or less.

(Water absorption rate of thermoplastic resin)
After the thermoplastic resin particles are uniformly dispersed on the polyester release film or aluminum foil, the polyester release film or aluminum foil is laminated on the polyester release film or aluminum foil to sandwich the thermoplastic resin particles, and then this laminate is hot-pressed. Then, the film is treated at 0.5 MPa for 2 minutes at a temperature 10 ° C. higher than the melting point of the thermoplastic resin particles, and then the release film or the aluminum foil is peeled off to prepare a film for measuring water absorption (thickness: about 1 mm). This film is immersed in water at 25 ° C. for 3 hours and then pulled up. The film is sandwiched in paper with good water absorption to remove water droplets on the surface, and the weight is measured. From the weight increase, the water absorption rate (= weight increase / before water absorption) Weight × 100).

The water absorption rate of the thermoplastic resin is used as an index of hydrophilicity of the thermoplastic resin, but the hydrophilicity of the thermoplastic resin can also be expressed by a water contact angle. The thermoplastic resin is formed into a film by the same method as the film for measuring water absorption described above, and the water contact angle measured by the θ / 2 method with respect to the film surface is 95 ° or less, particularly 85 ° or less. It is preferable. The water absorption rate described above does not necessarily correspond to this water contact angle, and there are thermoplastic resins having a low water absorption rate even if the water contact angle is small, and thermoplastic resins having a large water contact angle even if the water absorption rate is large. . When both of these characteristics are used as an index, the water absorption rate is large and the water contact angle is small, but the water contact angle is about 0.2%, even if the water absorption rate is relatively low. If it is small, it can be said that it is effective for the present invention.

The thermoplastic resin also preferably has an MFR measured by the following method of 55 g / 10 min or less. When the fluidity is high such that the MFR exceeds 55 g / 10 min, the surface of the porous body having mesopores is widely covered by the flow at the time of heat fusion, and the exposed area of the porous body having mesopores is reduced. In addition, the pores of the porous body having mesopores may be blocked, and the inherent absorption / desorption function of the porous body having mesopores may not be effectively exhibited. The thermoplastic resin preferably has a small MFR, but if the MFR is too small, the fluidity at the time of heat melting is too small, and it may be difficult to mold a porous body having mesopores. Therefore, the MFR of the thermoplastic resin is preferably 1 to 55 g / 10 min, particularly 5 to 50 g / 10 min.

(MFR of thermoplastic resin)
Measured according to JIS K6760 at 190 ° C. and 2.16 kg load.

Although there is no restriction | limiting in particular about melting | fusing point of a thermoplastic resin, Usually, 50 degreeC or more, Preferably it is 80 degreeC or more, and is usually 250 degrees C or less, Preferably it is 150 degrees C or less. When the melting point of the thermoplastic resin is less than 50 ° C., there is a problem that the thermoplastic resin is likely to be thermally deformed when used at room temperature. In the case where the water-permeable layer is provided, the water-permeable layer may be thermally deformed.

The shape of the thermoplastic resin is not particularly limited, but by using a particulate material, a porous body having mesopores and the thermoplastic resin are mixed and pressed to form a sheet that is an adsorption delayer. Can do. When thermoplastic resin particles are used, a suitable particle size exists in relation to the particle size of the porous body having mesopores. If the particle diameter of the thermoplastic resin particles is excessively large relative to the particle diameter of the porous body having mesopores, good voids cannot be formed between the porous bodies having mesopores, and the thermoplastic resin is formed during sheet formation. In some cases, a porous body having mesopores is buried in the resin particles, and a sufficient absorption / desorption function cannot be exhibited. Even if the particle diameter of the thermoplastic resin particles is excessively small, porous bodies having mesopores are in close contact with each other, and the porosity of the absorption / desorption layer is reduced. Thermoplastic resin particles having an excessively small particle size are difficult to obtain as a commercial product and have the disadvantage of being inferior in uniform mixing with a porous material having mesopores.

The average particle diameter of the thermoplastic resin particles is the average particle diameter of the thermoplastic resin particles / the average particle diameter of the porous body having mesopores = 1/8 to 15 / the average particle diameter of the porous body having mesopores. 1, particularly in the range of 1/7 to 8/1. The average particle diameter itself of the thermoplastic resin particles is arbitrary within the range satisfying the above average particle diameter ratio, but is preferably about 5 to 900 μm from the viewpoint of easy availability and handleability.

The average particle diameter of the thermoplastic resin particles is a value determined by a laser diffraction scattering method (water dispersion wet method).

When the absorption / desorption layer of the absorption / desorption material according to the embodiment of the present invention includes the above-described thermoplastic resin particles, the weight ratio of the thermoplastic resin particles constituting the absorption / desorption layer and the porous body having mesopores is It is preferably 1/4 to 4/1, particularly 1/3 to 3/1. If the thermoplastic resin particles are less than this range, the moldability of the porous body having mesopores is poor and it is difficult to form a sheet-like absorption / desorption layer. When there are more thermoplastic resin particles than this range, the resin melted at the time of molding a porous body having mesopores easily fills the voids between the particles, resulting in a decrease in porosity. Furthermore, if the resin enters or covers the pores of the porous body having mesopores, the absorption / desorption performance decreases, and an absorption / desorption material with excellent absorption / desorption performance can be obtained. Can not.

(Inorganic filler)
By including an inorganic filler having a specific gravity greater than that of the porous material having mesopores in the absorption / desorption layer, the specific gravity of the absorption / desorption material is increased, and it is easy to sink in biological fluids and other treatment liquids. The wettability with biological fluids and other treatment liquids can be improved. Examples of inorganic fillers used for such purposes include metal oxide particles such as zircon (zirconium silicate, specific gravity 4.7), quartz (specific gravity 2.7), alumina (specific gravity 4.0), stainless steel, titanium, and the like. One kind or two or more kinds of metal particles such as an alloy (Ti-6Al-4V) and a cobalt-chromium alloy (Vitalium, Co-Cr-Mo alloy) can be used. In addition, in the case of the metal particle used as the coagulation factor of blood, it is preferable to use it by surface coating with a bioinert material.

If the particle size of the inorganic filler is excessively large, the thickness of the absorption / desorption layer will be affected, and the porous body having mesopores will move easily in the water-permeable sheet, leading to powder leakage, and conversely If it is excessively small, it becomes easy to fill the voids of the porous body having mesopores and the absorption / desorption performance may be lowered. For example, a material having the same size as the porous body having mesopores is used. .

The inorganic filler is used according to the purpose so that the adsorbent / desorbing material has a desired specific gravity. However, in order to secure the content of the porous body having mesopores in the adsorbing / desorbing layer, the total volume is used. Is usually 80% or less, preferably 70% or less of the apparent volume of the adsorption / desorption layer, and is usually 5% or more.

When laminating the absorption / desorption layer and the air-permeable layer, the coarse silica particles are protruded from the surface of the water-permeable layer, which will be described later, by incorporating coarse particles of silica in the absorption / desorption layer. The wettability of the adsorbing / desorbing material can be enhanced by using the particles as hydrophilic starting points. In this case, the particle diameter of the coarse silica particles may be slightly larger than the thickness of the absorption / desorption layer, and is usually 1.5 to 5 times the thickness of the absorption / desorption layer. In the case where coarse particles of silica are contained in the absorption / desorption layer, if the amount is too small, sufficient wettability improvement effect cannot be obtained. However, if the amount is too large, the molding stability of the absorption / desorption layer may be impaired, or the absorption. -Since the amount of desorption is limited, the total volume is usually 80% or less with respect to the apparent volume of the absorption / desorption layer, preferably 70% or less, and usually 5% or more. The volume ratio.

The absorption / desorption layer preferably has a porosity of 10% or more. If the porosity of the absorption / desorption layer is too small, the penetration efficiency of biological fluids and various treatment liquids into the absorption / desorption layer is poor, and excellent absorption / desorption performance with a porous body having mesopores can be sufficiently obtained. It cannot be performed and the cleaning efficiency is inferior. The porosity is preferably 10% or more and is preferably high to some extent. However, if the porosity is excessively high, the ratio of the porous body having mesopores is relatively reduced, so that the absorption / desorption performance is lowered, and the porous body having mesopores In addition, since there are few thermoplastic resin particles and other materials, the moldability, shape retention, and functionality of the absorption / desorption layer are inferior. Accordingly, the porosity of the adsorption / desorption layer is usually 15% or more, preferably 20% or more, more preferably 30% or more, and usually 95% or less, preferably 90% or less, more preferably 85% or less. .

Here, the porosity of the adsorption / desorption layer is, for example, a value obtained by the following method in the case of an adsorption / desorption layer composed of a porous body having mesopores and thermoplastic resin particles.

(Porosity of absorption / desorption layer)
The porosity of the absorption / desorption layer refers to the void (however, the fineness of the porous body having mesopores) out of the volume formed between the sheets when a smooth surface sheet is laminated on both sides of the absorption / desorption layer. The volume of the constituent material occupying the absorption / desorption layer determined from the specific gravity of the material constituting the absorption / desorption layer and the weight used, and the appearance of the absorption / desorption layer It is calculated | required by calculation from the volume (volume formed between the water-permeable sheets mentioned later).
Specifically, the absorption / desorption layer of 12 cm × 12 cm (area) is calculated by the following method.
Absorption / desorption layer volume [cm ³ ]: 12 × 12 × (absorption / desorption layer thickness)
Volume of porous body having mesopores [cm ³ ]:
(Weight of absorption / desorption layer) × (weight ratio of porous body having mesopores in the material constituting absorption / desorption layer) / (true specific gravity of porous body having mesopores)
Pore volume [cm ³ ] of a porous body having mesopores:
(Adsorption / desorption layer weight) × (weight ratio of porous body having mesopores in the constituent material of absorption / desorption layer) × (pore volume [cm ³ ] of porous body having mesopores)
Thermoplastic resin particle volume [cm ³ ]:
(Weight of absorption / desorption layer) × (weight ratio of thermoplastic resin particles in the material constituting absorption / desorption layer) / (thermoplastic resin particle density)
Void volume [cm ³ ]:
(Adsorption and desorption layer volume [cm ^3] - particle volume of the porous body having mesopores [cm ^3] - pore volume of the porous body having mesopores [cm ^3] - thermoplastic resin particle volume ^[cm 3])
Absorption / desorption layer porosity [%]: (void volume [cm ³ ]) / (absorption / desorption layer volume [cm ³ ]) × 100

The thickness of the absorption / desorption layer is preferably thin from the viewpoint of the permeability and permeability of the liquid during the liquid contact treatment. Therefore, the thickness of the absorption / desorption layer is preferably 20 times or less, more preferably 10 times or less, and more preferably 5 times or less the average particle diameter of the porous body having mesopores contained in the layer. More preferably it is. Moreover, it is preferable that it is 2 times or more. That is, the thickness is preferably such that about 2 to 5 porous layers having mesopores are formed in the thickness direction of the absorption / desorption layer.
For example, the thickness of the absorption / desorption layer is preferably 100 μm or more, more preferably 200 μm or more, and preferably 2000 μm or less, more preferably 1500 μm or less.

<Water-permeable layer>
The absorption / desorption material according to the embodiment of the present invention may have a water-permeable layer. The water permeable layer is not particularly limited as long as it is a material having excellent hydrophilicity, water permeability, and biocompatibility and satisfying mechanical strength, chemical resistance, etc. required for handling. Or it is preferable to use a nonwoven fabric.
Although a commercially available thing can be used as a woven fabric or a nonwoven fabric, It is preferable to use a nonwoven fabric as a water-permeable layer from the point which is especially cheap and can obtain easily the thing by which opening adjustment was carried out.

Examples of fibers constituting the nonwoven fabric or woven fabric used in the water-permeable layer include polyester fibers such as polyethylene terephthalate and polytrimethylene terephthalate; polyacrylic fibers; polyolefin fibers such as polyethylene and polypropylene; polyamide fibers such as nylon 6 and nylon 66 Such synthetic fibers; those obtained by combining one or more semi-synthetic fibers such as rayon and acetate fibers can be used. Of these, semi-synthetic fibers, particularly rayon, are preferable because they have high wettability, and are easy to handle when desorbing and desorbing, and polyolefin fibers are preferable because of high recovery of biological materials. Polyester fibers are preferable in that they satisfy a good balance between wettability and biological material recovery.
In addition, fibers with heat-fusibility as a core-sheath structure, special fibers with increased hydrophilicity, such as ultra-fine fibers, irregular fiber cross-sections, grooves and recesses on side surfaces, and fiber porosity Further, surface-treated fibers subjected to hydrophilic coating treatment can also be used. In particular, the core-sheath fiber is preferable because it can be easily heat-sealed at the time of cutting when the absorption / desorption material of the present invention described later is produced.

The water-permeable layer preferably has a larger opening due to the permeability and permeability of various liquids during the liquid contact treatment, but if it is excessively large, the molding stability of the absorption / desorption layer will be inferior.
Further, the thickness of the water-permeable layer is preferably thicker from the viewpoint of securing the strength of the absorbent / desorbing material and ease of heat fusion, and thinner for thinner.
The water-permeable layer preferably satisfies the following conditions from the standpoints of the permeability, permeability, various strengths of the absorbent / desorbent, and handleability of various liquids during the liquid contact treatment.

The average opening of the water-permeable layer is usually 1 μm or more, preferably 10 μm or more, and usually 300 μm or less, preferably 200 μm or less. The average opening of the water-permeable layer is usually 0.1 times or more, preferably 0.2 times or more, and usually 1 time or less with respect to the average particle diameter of the porous body having mesopores in the absorption / desorption layer. , Preferably 0.8 times or less.

The thickness of the water-permeable layer is usually 50 μm or more, preferably 70 μm or more, more preferably 100 μm or more, and usually 1000 μm or less, preferably 500 μm or less, more preferably 300 μm or less. Weight of the water-permeable layer (g / ^{m 2)} is 1/10 to 10 times the weight of the porous body having mesopores of adsorption and desorption layer (g / ^{m 2),} and in particular 1 / 5-5 times It is preferable to use it as follows.
When the water-permeable layer is a nonwoven fabric, the basis weight is usually 10 g / m ² or more, preferably 20 g / m ² or more, more preferably 30 g / m ² or more, and usually 200 g / m ² or less, preferably It is 150 g / m ² or less, more preferably 100 g / m ² or less.

The water-permeable layer has an air permeability measured according to JIS L 1096 6.27.1 A method (Fragile method) of usually 50 cc / cm ² · sec or more, preferably 70 cc / cm ² · sec or more, Further, it is usually 250 cc / cm ² · sec or less, preferably 200 cc / cm ² · sec or less.

Further, the specific surface area of the porous body having mesopores in the absorption / desorption layer with respect to the specific surface area of the water-permeable layer is usually 1000 times or more, preferably 10,000 times or more, and usually 10 million times or less. Here, the specific surface area of the water-permeable layer is the value of the surface area of the yarn (fiber) to be formed when the water-permeable layer is composed of a woven fabric or a non-woven fabric, and the specific surface area of the porous body having mesopores. This is the total value of the surface area outside the particle and the surface area inside the pore, and can be determined by the nitrogen gas BET method.

When the water-permeable layer is composed of a woven or non-woven fabric, if the fibers constituting the woven or non-woven fabric are highly hydrophobic and sufficient wettability cannot be obtained, the following hydrophilicity improving treatment is applied. May be.
(1) A surface treatment using a plasma such as argon or oxygen is performed on a woven fabric or a non-woven fabric to introduce a hydroxyl group to enhance hydrophilicity.
(2) Improve wettability and hydrophilicity by increasing the surface roughness of the woven or non-woven fabric with sandblasting or the like. By this blasting treatment, a hydrophilic element can be implanted into the surface of the woven or non-woven fabric, thereby further improving the hydrophilicity.
(3) Hydrophilic copolymer resin using hydrophilic fibers such as cellulose fiber, rayon fiber, polyvinyl alcohol (PVA) fiber, ethylene vinyl acetate copolymer (EVA) fiber, or hydrophilic monomers such as vinyl acetate and vinyl alcohol A fiber, etc., as a fiber constituting a woven fabric or a nonwoven fabric, alone or in addition to those exemplified above as a fiber constituting a nonwoven fabric or a woven fabric used for a water-permeable sheet, mixed with these fibers, Alternatively, it is used by a method of laminating or forming a sheet using a binder or the like as a short fiber.
(4) A treatment for physically or chemically fixing hydrophilic groups such as polyethylene glycol, poly (2-hydroxyethyl methacrylate), poly (acrylamide), and sulfonated segment polyurethane to the surface of the woven or non-woven fabric is performed.
(5) The surface of the woven or non-woven fabric is subjected to a silica coating treatment to make it hydrophilic. This silica coating may be performed partially by printing dots or lines on a woven or non-woven fabric.

The degree of hydrophilicity of the water-permeable layer is such that when a 1 cm square sheet is immersed in water in a form that floats on the water surface, it is buried in water in a short time. The hydrophilicity is preferably such that it is buried within 1 minute, more preferably within 30 seconds. In addition, as another method for indicating the degree of hydrophilicity, when water droplets of pure water are dropped on the water-permeable layer, water starts to penetrate into the sheet within 10 seconds, preferably within 5 seconds. It should be soaked in water.

In addition, as a specific gravity adjustment to make it easier for the absorbent / desorbent material to sink in biological fluids and various processing liquids, a perforated sheet (for example, punching metal) made of a material having a high specific gravity is laminated on the water-permeable layer, A specific gravity material may be included.

It is preferable that the water-permeable layer does not become clogged with contaminants or adhere to an adhesive when the absorbent / desorbent is immersed in the biological fluid. Moreover, it is preferable that it is excellent in hydrophilicity from the surface of the permeability of various liquids at the time of a liquid-contact process, and a permeability. However, depending on the material of the water-permeable layer and the surface treatment method, the amount of biological material adsorbed on the water-permeable layer is excessive, and the biological material in the pores of the porous body having mesopores in the absorption / desorption layer It is preferable to adjust the surface state as appropriate because there is a possibility that the amount of adsorbed will be reduced.
For the adjustment of the surface condition, for example, an antithrombotic material is preferably applied, and examples of the antithrombotic material include heparin-immobilized coating material, urokinase-immobilized coating material, thrombomogerin-immobilized coating material, and segmentation. Examples include polyurethane coating materials, synthetic biocompatible coating materials, phospholipid adsorption or immobilization coating materials (MPC polymers), and alkylated cellulose coating materials.

In addition, although there is a demerit that it becomes hydrophobic and lowers hydrophilicity, it can be made excellent in biocompatibility. Therefore, a silicone material such as polydimetalsiloxane can be used for the aqueous layer or a silicone coating can be passed. It may be applied to the aqueous layer.
Fluorine resins such as polyethylene and polyolefins typified by polypropylene and polytetrafluoroethylene can be preferably used as a constituent material of the water-permeable layer and also as a coating material.

<Configuration of Absorption / Desorption Material of the Present Invention>
A specific structural example of the adsorbing / desorbing material of the present invention will be described below with reference to the drawings. The following description is an example of the absorbing / desorbing material of the present invention, and the adsorbing / desorbing material of the present invention is It is not limited to the following.

FIG. 1 is a schematic cross-sectional view showing an example of an embodiment of the adsorption / desorption material of the present invention.
FIG. 1A is an example of a preferred embodiment of a sheet including a porous body having mesopores. The absorbent / desorbing material 1 is a sheet-like absorbent / desorbing material in which a porous body 2 having mesopores is bonded to each other with a binder 3. A water-permeable layer 5 is laminated on both surfaces of the desorption layer 4. The absorption / desorption layer 4 and the water-permeable layer 5 are bonded together by a binder 3 included in the absorption / desorption layer 4.
The thickness of the absorbent / desorbable material in which the water-permeable layer 5 is laminated on both surfaces of the absorbent / desorbable layer 4 is usually 300 μm or more, preferably 500 μm or more, and usually 5000 μm or less, preferably 4000 μm or less. Preferably it is 3000 micrometers or less.

In FIG. 1 (a), the absorption / desorption layer 4 is composed of a porous body 2 having mesopores and a binder 3. May be included. The water-permeable layers 5 on both surfaces of the absorption / desorption layer 4 may be made of the same material or different materials in one water-permeable layer and the other water-permeable layer.
In the structure in which the water-permeable layer 5 is laminated on both surfaces of the absorption / desorption layer 4, the absorption / desorption layer 4 may or may not include the binder 3.
When a binder is included, a sheet-like absorption / desorption layer 4 in which porous bodies 2 having mesopores are bonded to each other with a binder 3 is formed as shown in FIG.
When the binder is not included, the (ai) absorption / desorption layer is a porous molded body having mesopores molded without the binder, and (aii) the absorption / desorption layer is a mesopore. (A-iii) Absorption / desorption layer, which is a layer formed by passing a porous body having pores to a water-permeable layer, is formed by sandwiching a porous body having mesopores between water-permeable layers. The adsorbing / desorbing material can be formed into a sheet shape by a mode such as maintaining

(Ai) When the absorption / desorption layer is a porous molded body having mesopores molded without containing a binder, the molded body, for example, compresses and molds a porous body having mesopores. Can be obtained. When the absorption / desorption layer does not contain a binder, the absorption / desorption layer and the water-permeable layer may be laminated using the same material as the binder described above, and heat fusion is performed by the water-permeable layer containing a material that can be fused. You may wear it. In this embodiment, the absorption / desorption layer is likely to be brittle, while the amount of the binder can be reduced, and the liquid containing the biological material can be efficiently brought into contact with the porous body having mesopores. preferable.

(A-ii) In the case where the absorption / desorption layer is a layer formed by fixing a porous body having mesopores to the aqueous layer, in order to form the absorption / desorption layer, A porous body having mesopores may be fixed only to one layer, or a porous body having mesopores may be fixed to both water-permeable layers. It is preferable to fix the porous body having mesopores to both water-permeable layers in that the amount of the porous body having mesopores contained in the adsorbing / desorbing material is easily increased. The fixing method is not limited. For example, a porous body having mesopores and a water-permeable layer may be bonded to the water-permeable layer with a binder and a material that can be fused to the water-permeable layer is used. And may be fused. In this embodiment, since two water-permeable layers are not adhere | attached through an absorption / desorption layer, it is necessary to fix two water-permeable layers mutually. The fixing position of the two water-permeable layers is not limited, and examples thereof include a method of fixing the peripheral portions of the two water-permeable layers and a method of fixing the two water-permeable layers at a predetermined interval. The fixing method is not limited, and may be, for example, adhesion or heat fusion. In the case of bonding, the aforementioned binder may be used as an adhesive. In the case of fusion, the water-permeable layer is preferably made of a material that can be heat-sealed. The fixing position is not limited, but it is preferable to join the peripheral edge of the water-permeable layer in order to prevent powder leakage. According to this embodiment, since the area in contact with the binder is reduced on the surface of the porous body having mesopores, the liquid contact treatment can be efficiently performed, and the porous body 2 having mesopores is water-permeable. Since it is fixed to the layer, it is preferable in that it is difficult for the powder to leak.

(A-iii) When the absorption / desorption layer holds the shape of the layer by sandwiching the porous body having mesopores with the water-permeable layer, the porous body having mesopores flows during the liquid contact treatment, Like this, there is a risk of powder leakage. The aspect which fixed the peripheral part of the two water-permeable layers so that a porous body may not leak from the peripheral part of a water-permeable layer is preferable. The method for fixing the peripheral portions of the two water-permeable layers is not limited, and for example, adhesion or fusion may be used. In the case of bonding, the aforementioned binder may be used as an adhesive. Two water-permeable layers may be fused at a predetermined interval. In this case, it is preferable to use a material that can be heat-sealed to the water-permeable layer.

In addition, it is possible to devise the design so that the fluidity of the mesoporous silica particles in the adsorption / desorption layer becomes appropriate while ensuring the permeability and permeability of various liquids during the liquid contact treatment. preferable.
Even if the absorption / desorption layer 4 includes the binder 3, the porous body 2 having mesopores may fall off from the absorption / desorption layer 4 to cause powder leakage, thereby suppressing the powder leakage. In order to do so, it is preferable to devise a design.
The embodiment of FIG. 1A in which the absorption / desorption layer 4 includes the binder 3 and the water-permeable layer 5 is laminated on both surfaces of the absorption / desorption layer 4 particularly suppresses the powder from leaking from the absorption / desorption material 1. This is a preferred embodiment, and is preferred in that it is difficult to inhibit the stable operation of the biological material analyzer.

FIG. 1B is an example of a preferred embodiment of an absorbent / desorbing material in which an absorbing / desorbing layer including a porous body having mesopores and a water-permeable layer are laminated. Porous bodies 2 having mesopores are bonded to each other with a binder 3 to form a sheet-like absorption / desorption layer 4, and a water-permeable layer 5 is laminated on one surface of the absorption / desorption layer 4. The absorption / desorption layer 4 and the water-permeable layer 5 are bonded together by a binder 3 included in the absorption / desorption layer 4.
The water-permeable layer 5 is laminated on one surface of the absorption / desorption layer 4, that is, the thickness of the absorption / desorption material when one absorption / desorption layer and one water-permeable layer are laminated, Usually, it is 150 μm or more, preferably 200 μm or more, and usually 3000 μm or less, preferably 2000 μm or less.
In addition, the absorption / desorption layer may be laminated | stacked on both surfaces of the water-permeable layer. In this case, the thickness of the absorption / desorption material is usually 250 μm or more, preferably 400 μm or more, and is usually 5000 μm or less, preferably 3000 μm or less, more preferably 2000 μm or less.
In FIG. 1 (b), the adsorption / desorption layer 4 is composed of a porous body 2 having a mesopore and a binder 3. Further, the adsorption / desorption layer 4 according to the present invention is coarse in the inorganic filler and silica described above. It may contain particles.
In the configuration in which the water-permeable layer 5 is laminated on one surface of the absorption / desorption layer 4, the absorption / desorption layer 4 may or may not include the binder 3.
When a binder is included, a sheet-like absorption / desorption layer 4 in which porous bodies 2 having mesopores are bonded to each other with a binder 3 is formed as shown in FIG.
When the binder is not included, (bi) the absorption / desorption layer is a porous molded body having mesopores molded without the binder, (b-ii) the absorption / desorption layer is a mesopore The absorbing / desorbing material can be formed into a sheet shape by an embodiment such as a layer formed by passing a porous body having pores to the aqueous layer.

(Bi) When the absorption / desorption layer is a porous molded body having mesopores molded without containing a binder, the molded body is formed by, for example, tableting a porous body having mesopores. It is obtained with. When the absorption / desorption layer does not contain a binder, the same material as the binder described above may be used for the lamination of the absorption / desorption layer and the water-permeable layer. May be. In the present embodiment, the absorption / desorption layer tends to be brittle, while the amount of the binder can be reduced, and the liquid containing the biological material can be efficiently brought into contact with the porous body having mesopores. preferable.

(B-ii) When the absorption / desorption layer is a layer formed by fixing a porous body having mesopores to the aqueous layer, the fixing method is not limited. For example, a porous body having mesopores The porous body having mesopores and the water-permeable layer may be fused using a material that can be adhered to the water-permeable layer with a binder and can be fused to the water-permeable layer. According to this embodiment, since the area in contact with the binder is reduced on the surface of the porous body having mesopores, the liquid contact treatment can be efficiently performed, and the porous body 2 having mesopores is water-permeable. Since it is fixed to the layer, it is preferable in that it is difficult for the powder to leak.
In the embodiment of FIG. 1B in which the absorption / desorption layer 4 includes the binder 3 and the water-permeable layer 5 is laminated on the absorption / desorption layer 4, the adhesion / desorption layer 1 is prevented from leaking. Various liquids at the time of the liquid treatment are preferable in that they easily come into contact with the absorption / desorption layer 4.

FIG. 1 (c) is an example of a preferred embodiment of the adsorbing / desorbing material 1 having a layer including a porous body 2 having mesopores and a binder 3. The porous body 2 having mesopores adheres to each other with the binder 3. Thus, a sheet-like absorption / desorption material 1 is formed.
The thickness of the absorbing / desorbing material in a form in which the water-permeable layer is not laminated on the absorbing / desorbing layer is usually 100 μm or more, preferably 200 μm or more, and usually 2000 μm or less, preferably 1000 μm or less.
In the embodiment of FIG. 1 (c), various components at the time of the liquid contact treatment are easily brought into contact with the absorbing / desorbing layer 4, and the component to which the biological material can adhere other than the porous body 2 having mesopores is only the binder 3. Therefore, it is preferable in that the recovery rate of the biological material can be easily increased and various liquids during the liquid contact treatment can be efficiently brought into contact with the absorption / desorption layer 4.

The shape of the sheet or laminate of the absorbent / desorbent material of the present invention is not particularly limited, and is appropriately determined according to the purpose, such as the form of the biological fluid analyzer to be applied. Usually, the sheet means a shape having a width and depth larger than the thickness, but in the present invention, the shape of the sheet or laminate includes a shape having a thickness larger than the width and / or depth. For example, it may be a rectangular parallelepiped whose thickness is longer than the length of one side of the bottom surface, a cylinder whose thickness in the stacking direction is larger than the diameter of the bottom surface, or a cube having the same thickness, width, and depth. It may be. From the viewpoint of handleability, a shape having a larger width and depth than thickness is preferable.
For example, the suction / desorption material 1 of the present invention is inserted into a blood collection spitz (test tube) 10 as shown in FIG. 2A and used in contact with the blood 6 in the spitz 10. In accordance with the shape of the Spitz 10, a substantially rectangular shape in which one end portion becomes narrower toward the tip end side may be used.

Further, in such an adsorbing / desorbing material, in order to enhance the liquid permeability of the processing liquid, as shown in FIG. 2B, the absorbing / desorbing material 1A having a through hole 1a in the thickness direction, as shown in FIG. As shown to (c), it is good also as the absorption / desorption material 1B which put the cut 1b in the both sides | edges.

Further, in order to increase the amount of the suction / desorption material sheet that can be inserted into the spitz and increase the suction / desorption area, as shown in FIG. It is good.

Furthermore, a configuration may be adopted in which a small piece 1D of the absorbent / desorbing material is placed in the net 7 and suspended by a hanging strap 8 or the like like a tea bag.

The adsorbing / desorbing material can be manufactured, for example, by the manufacturing method described below. In order to reliably prevent the porous body having mesopores in the absorbing / desorbing layer from falling off, the adsorbing / desorbing layer is used. 3 by extending the periphery of two water-permeable sheets provided on both sides of the sheet to the outside of the periphery of the absorbing / desorbing layer and heat-sealing the extended portions by heat sealing or the like. As shown in (e), it is preferable that the edges of the water-permeable layer 22 and the water-permeable layer 24 are in close contact with each other at the entire peripheral edge portion of the absorbent / desorbable material 26 to form a sealed bag. The width of the heat-sealed portion between the water-permeable layers at the peripheral edge (the length L in FIG. 3 (e)) ensures sufficient heat-sealing strength, and absorbs / desorbs the area of the absorbing / desorbing material. From the viewpoint of increasing the layer area ratio and increasing the absorption / desorption efficiency, it is preferably 50 μm or more, and more preferably 2000 μm or less.

<Manufacturing method of absorption / desorption material>
The production method of the adsorbing / desorbing material is not limited. For example, the adsorbing / desorbing material can be produced as follows.
First, as shown in FIG. 3A (perspective view), a mask 21 in which a desired pattern is perforated is prepared, and the mask 21 is formed as a water-permeable layer as shown in FIG. 3B (cross-sectional view). A mesoporous silica particle layer 23 is formed in a pattern shape of the through holes 21a of the mask 21 on the water-permeable layer 22 by spreading a porous body having mesopores, for example, mesoporous silica particles, from above. . Next, as shown in FIG. 3C (cross-sectional view), the water-permeable layer 24 is placed thereon to form a laminate 25 of the water-permeable layer 21 / mesoporous silica particle layer 23 / water-permeable layer 24. Next, as shown by a one-dot chain line in FIG. 3D (plan view), by cutting between the mesoporous silica particle layers 23 of the obtained laminate 25 while heating, the lower water-permeable layer 22 and the upper water-permeable layer 22 are separated from each other. The water-permeable layer 24 is thermally fused and cut, and as shown in FIG. 3E (cross-sectional view), a sheet-like absorbent / desorbing material having a desired shape including the mesoporous silica particles 23A as the absorbing / desorbing layer. 26 is obtained. Further, by performing pleating, an absorbent / desorbing material 1C shown in FIG. 2 (d) can be obtained.
In addition to the method of heating and cutting at the same time as described above, a method of performing heat fusion by heating and then returning to room temperature and then cutting is suitably employed.

When the above-mentioned inorganic filler and silica coarse particles are contained together with the porous material having mesopores in the adsorption / desorption layer, the porous material having mesopores and the inorganic filler and / or Alternatively, a mixture of coarse silica particles may be sprayed.

In addition, when the porous body having mesopores and the above-mentioned binder are included in the absorption / desorption layer, the porous body having mesopores and, for example, thermoplastic resin particles as a binder when spraying the porous body having the above-mentioned mesopores. A mixture 25 is sprayed to form a laminate 25 as described above. After this laminated body 25 is heated by a hot press, a heating roll, a heating belt or the like, the porous bodies having mesopores and the porous body having mesopores and the water-permeable layer are bonded and integrated by melting the thermoplastic resin. Then cut as above.

In the manufacturing process of such an absorption / desorption layer, a mixture of a porous body having mesopores and a binder as needed is sprayed on a water-permeable layer sent out on a belt through a mask, and separately on the mixture. It can implement in the continuous process which piles up the water-permeable layer sent out and heats and cut | disconnects.

[Biological material purification kit]
The absorption / desorption material of the present invention can be used for absorption / desorption of a biological material from a liquid containing the biological material. Therefore, for example, an absorbent / desorbing material can be stored in a container for holding a liquid containing a biological material to obtain a biological material purification kit.
The biological material purification kit may include an absorption / desorption material and a container capable of holding the liquid containing the biological material, and the absorption / desorption is performed inside the container for holding the liquid containing the biological material during use. The material may be simply stored (inserted) for use.

Although the material of the container is not limited, it is preferably transparent in order to carry out a biological material absorption / desorption operation, and resin and glass are preferably exemplified. On the other hand, a colored container may be used for the purpose of preventing the biological material from being decomposed by light.
The volume of the container is not limited, but is usually 0.1 ml or more, preferably 0.5 ml or more, and the upper limit is not limited, but is usually 1000 L or less, preferably 100 L or less, more preferably 10 L or less. Any volume can be selected according to the purpose.
Although the shape of the container is not limited, it is preferable that the shape of the container and the shape of the adsorbing / desorbing material are shapes that can efficiently perform the liquid contact treatment.
When used for analysis of biological materials, examples of the container include a test tube, particularly a microtube.

[Production method of biological material / Separation method of biological material]
The absorption / desorption material according to the embodiment of the present invention can be applied to a known method of absorbing / desorbing and separating a biological substance from a liquid containing the biological substance. For example, the adsorbing / desorbing material according to the embodiment of the present invention is brought into contact with a liquid containing a biological material, and the biological material is selectively adsorbed in the mesopores of the porous body having mesopores, and then the absorbing / desorbing agent. The biological material can be obtained by washing and desorbing the biological material.

For example, when separating a peptide from a biological fluid, specifically blood, the absorption / desorption material is brought into contact with the blood or plasma obtained by processing the blood. The liquid with which the adsorbing / desorbing material is brought into contact preferably has a small content of impurities other than the target biological substance, and known methods can be used for removing the impurities.
After selectively adsorbing peptides in blood or plasma into mesopores of a porous body having mesopores in the adsorption / desorption layer, the adsorption / desorption material is pulled up, as described in Patent Document 1, After washing with water, the porous body is treated with an acidic buffer solution to peel and remove proteins and long-chain peptides adsorbed on the outer surface of the porous body having mesopores, and then treated with a peptide recovery solvent containing acetonitrile. By desorbing the peptide (short chain peptide) adsorbed in the mesopores, the peptide can be separated and recovered from the biological fluid to produce a high purity peptide.

The peptide separation method using the adsorbent / desorbent according to the embodiment of the present invention is effective for blood tests and peptide analysis, and easily and efficiently automates the analysis of the peptide from the biological fluid with a high recovery rate. Separation and collection enable high-precision analysis. For this reason, it is useful for early detection and diagnosis of cancer, hepatitis, and other diseases. It can also be effectively applied to the recovery and analysis of glycoproteins and glycopeptides.

In addition, since the absorption / desorption material according to the embodiment of the present invention can produce a high-purity peptide, in addition to such test purposes, lifestyle-related disease prevention, blood pressure reduction / stabilization, lipid reduction / stability This is useful for the production of supplements that have effects such as aging and anti-aging.

The present invention will be described more specifically with reference to the following examples.
[Example 1]
The following were used as a porous body having mesopores, a binder, and a water-permeable layer.
<Porous body having mesopores: mesoporous silica particles>
“Meso Pure” manufactured by Nippon Kasei Co., Ltd.
Particle size distribution: 70-600μm
Average particle size: 250 μm
Shape: Crushed pore size: 4 nm
Pore volume: 0.7 ml / g
Specific surface area: 730 m ² / g

<Binder: Thermoplastic resin particles>
EVA (ethylene vinyl acetate copolymer)
Water absorption rate: 0.09%
Water contact angle: 91 °
MFR: 70g / 10min
Melting point: 97 ° C
Average particle size: 40 μm

<Water-permeable layer>
Nonwoven fabric “ZL4035”
Constituent fiber: 30% rayon / 70% polyester Fiber average opening: 60 μm
Thickness: 220 μm
Per unit weight: 50 g / m ²
Fiber density: 152.3 kg / m ²
Specific surface area: 0.0003 m ² / g

The mixed powder was obtained by mixing in a mixing container and mixing well so that the weight ratio of mesoporous silica / binder would be 7/3. The obtained mixed powder was uniformly placed on the water-permeable layer so that the basis weight of mesoporous silica was 1000 g / m ^2, and the water-permeable layer was further covered thereon to form a sandwich structure.
The obtained sandwich structure was thermocompression-bonded at a press pressure of 0.5 MPa, a press temperature of 110 ° C., and a press time of 4 minutes using a desktop test press machine (manufactured by Shinto Metal Industry Co., Ltd.) to produce a molded sheet. .
A portion corresponding to 25 mg of mesoporous silica was cut out from the formed molded sheet, and a molded body sample 1 was obtained.

[Test Example 1]
<Preparation of peptide solution>
The peptide used was 0.5 mg of ACTH Human 1-24 Model No. 4109-v manufactured by Wako Pure Chemical Industries, Ltd. 25 μL of ultrapure water was placed in the reagent bottle, and all of the peptide was dissolved in the droplet to prepare a peptide solution.

<Method for quantifying peptides>
The peptide quantification method is shown below.
For peptide quantification, the following LC / MS analyzer was used to evaluate the peptide peak area ratio in the obtained chromatogram.
LC / MS measuring instrument;
LC system: Agilent 1200 manufactured by Agilent Technologies
Column: Imtakt Presto FT-C18 manufactured by Intact Corporation 4.6 mm × 30 mm
Mass spectrometer: Agilent LC / MS 6130 manufactured by Agilent Technologies, Inc.

The molded body sample 1 obtained in Example 1 was placed in an Eppendorf tube having a volume of 1.5 ml, and all the peptide solutions prepared above were further added thereto.
The Eppendorf tube was stirred for 1 hour at a rotation speed of 1500 rpm using a vortex mixer (As One Corporation model VM-96B), and the peptide solution was adsorbed to the molded body sample 1.
Thereafter, the molded body sample 1 was taken out from the Eppendorf tube and transferred into a 1 ml pipette tip, and then the pipette tip was attached to the quantitative pipette. After 1 ml of ultrapure water was sucked into the pipette tip containing the molded body sample 1, it was discharged to discharge the water and washed with water. This washing operation was repeated 5 times.
Next, 1 ml of 0.1 mol / L glycine-hydrochloric acid buffer solution (pH 2.2) manufactured by Wako Pure Chemical Industries, Ltd. was sucked up and then discharged to discharge the buffer solution, followed by washing with the buffer solution. This washing operation was repeated 5 times. The peptide adsorbed on the outer surface of the silica particles was peeled and removed by treatment with this water washing and acidic buffer solution.

Thereafter, the pipette tip was removed from the quantitative pipette, and the molded body sample 1 in the pipette tip was taken out and transferred into an Eppendorf tube having a volume of 1.5 ml. 1 ml of a 0.1 vol% trifluoroacetic acid-acetonitrile solution manufactured by Wako Pure Chemical Industries, Ltd. was added to the Eppendorf tube, and stirring with a vortex mixer (rotation speed: 1500 rpm) was performed for 30 seconds.
Then, after standing at room temperature for 1 hour, the molded body sample 1 was taken out with tweezers, and the remaining liquid was used as analysis sample 1. By this operation, the peptide adsorbed in the mesopores of silica was desorbed and eluted to be recovered.
This analysis sample 1 was quantified by the peptide quantification method described above. The results are shown in Table 1.

[Comparative Example 1]
25 mg of the mesoporous silica used in Example 1 was weighed and placed in an Eppendorf tube having a volume of 1.5 ml, and the peptide solution was added thereto.
The Eppendorf tube was stirred for 1 hour at a rotation speed of 1500 rpm using a vortex mixer (As One Corporation model VM-96B) to adsorb the peptide solution onto mesoporous silica.
Thereafter, 1 ml of ultrapure water was added to the Eppendorf tube, and the mixture was stirred for 30 seconds using the vortex mixer (rotation number: 1500 rpm). Thereafter, the Eppendorf tube was centrifuged for 10 minutes using a centrifuge (manufactured by CHIBITAN-R Merck). Thereafter, after removing the supernatant, 1 ml of ultrapure water was added, and the above-described vortex mixer stirring and centrifugation were repeated three times.

Thereafter, 1 ml of 0.1 mol / L glycine-hydrochloric acid buffer (pH 2.2) manufactured by Wako Pure Chemical Industries, Ltd. was added, and the mixture was stirred and centrifuged with a vortex mixer in the same manner as when adding ultrapure water. Repeated 3 times. The peptide adsorbed on the outer surface of the silica particles was peeled and removed by treatment with this water washing and acidic buffer solution.
Next, 1 ml of 0.1 vol% trifluoroacetic acid-acetonitrile solution manufactured by Wako Pure Chemical Industries, Ltd. was added, and stirring with a vortex mixer (rotation speed: 1500 rpm) was performed for 30 seconds.
Thereafter, the sample was allowed to stand at room temperature for 1 hour, centrifuged for 10 minutes with the above-mentioned centrifuge, and the supernatant was collected to obtain an analytical sample 1 ′. By this operation, the peptide adsorbed in the mesopores of silica was desorbed and eluted to be recovered.
This analysis sample 1 ′ was quantified by the above-described peptide quantification method. The results are shown in Table 1.

[Comparative Example 2]
Peptide absorption / desorption evaluation was performed in the same manner as in Comparative Example 1 except that the mesoporous silica particles shown below were used. The results are shown in Table 1.
<Porous body having mesopores: mesoporous silica particles>
“Meso Pure” manufactured by Nippon Kasei Co., Ltd.
Particle size distribution: 1-10μm
Average particle size: 5 μm
Shape: Fine powder pore size: 4 nm
Pore volume: 0.6 ml / g
Specific surface area: 720 m ² / g

[Example 2]
A molded body sample 2 was prepared in the same manner as in Example 1 except that the water-permeable layer shown below was used, and an analytical sample 2 was obtained. This analytical sample 2 was quantified by the peptide quantification method described above. The results are shown in Table 1.
<Water-permeable layer>
Non-woven fabric “TM022D” manufactured by Kuraray Co., Ltd.
Constituent fiber: Polypropylene fiber thickness: 188 μm
Weight per unit: 23 g / m ²

[Example 3]
A molded body sample 3 was prepared in the same manner as in Example 1 except that the mesoporous silica shown below was used, and an analytical sample 3 was obtained. This analysis sample 3 was quantified by the peptide quantification method described above. The results are shown in Table 1.
<Porous body having mesopores: silica gel>
“Carriert G-6” manufactured by Fuji Silysia Chemical Ltd.
Particle size distribution: 45 to 150 μm
Shape: Crushed pore size: 6 nm
Pore volume: 0.8ml / g
Specific surface area: 560 m ² / g

From the comparison between Comparative Example 1 and Example 1, the use of the adsorbing / desorbing material of the present invention in the form of a sheet increases the amount of peptide detected rather than using the adsorbing / desorbing material in powder form. It is shown. Moreover, it is clear from the comparison between Example 1 and Example 2 that the amount of peptide recovered is larger when PP (polypropylene) is used as the water-permeable layer.
Further, Example 3 using silica gel as a porous material having mesopores has a larger amount of peptide detected than Example 1 using mesoporous silica. Since the types of porous bodies are different, direct comparison is not possible, but the porous body of Example 3 has a smaller particle size than the porous body of Example 1. Since the porous body has a larger surface area that comes into contact with the peptide-containing solution when the particle size is smaller, the volume of the porous body that can contribute to the absorption and desorption of the peptide increases, and it is considered that the recovered amount of peptide is large.

[Example 4]
A model experiment was conducted to examine the effect of the thickness of the water-permeable layer on the amount of peptide recovered. An analysis sample 4 was obtained in the same manner as in Example 1 except that the nonwoven fabric used for the water-permeable layer was cut into a size having the same area as both surfaces of the molded body and tested together with the molded body sample. This analytical sample 4 was quantified by the peptide quantification method described above. The results are shown in Table 2.

[Example 5]
An analysis sample 5 was obtained in the same manner as in Example 1 except that the nonwoven fabric used for the water-permeable layer was cut into a size having the same area as that of one side of the molded body and tested together with the molded body sample. This analytical sample 5 was quantified by the peptide quantification method described above. The results are shown in Table 2.

[Example 6]
A molded body sample 5 was produced in the same manner as in Example 1 except that the mesoporous silica shown below was used, and an analytical sample 6 was obtained. This analytical sample 6 was quantified by the peptide quantification method described above. The results are shown in Table 2.
<Porous body having mesopores: mesoporous silica particles>
“Meso Pure” manufactured by Nippon Kasei Co., Ltd. used in Example 1 was classified to obtain a porous body having the following particle size distribution and average particle size.
Particle size distribution:> 425 μm
Average particle diameter: 566 μm
Shape: Crushed pore size: 4 nm
Pore volume: 0.7 ml / g
Specific surface area: 730 m ² / g

[Example 7]
A molded body sample 7 was produced in the same manner as in Example 1 except that the water-permeable layer shown below was used, and an analytical sample 7 was obtained. This analytical sample 7 was quantified by the peptide quantification method described above. The results are shown in Table 2.
<Water-permeable layer>
Non-woven fabric “FV-4365” manufactured by Japan Vilene Co., Ltd.
Constituent fiber: Polyester fiber

[Example 8]
Except using the water-permeable layer shown below, the molded object sample 8 was produced similarly to Example 1, and the analysis sample 8 was obtained. This analytical sample 8 was quantified by the peptide quantification method described above. The results are shown in Table 2.
<Water-permeable layer>
Nonwoven fabric "ELTRS-N" manufactured by Asahi Kasei Corporation
Constituent fiber: Nylon fiber

[Example 9]
Except using the water-permeable layer shown below, the molded object sample 9 was produced similarly to Example 1, and the analysis sample 9 was obtained. This analytical sample 9 was quantified by the peptide quantification method described above. The results are shown in Table 2.
<Water-permeable layer>
Nonwoven fabric "ELTRS-P" manufactured by Asahi Kasei Corporation
Constituent fiber: Polypropylene fiber

[Example 10]
A molded body sample 10 was produced in the same manner as in Example 1 except that the binder shown below was used, and an analytical sample 10 was obtained. This analytical sample 10 was quantified by the peptide quantification method described above. The results are shown in Table 2.
<Binder: Thermoplastic resin particles>
Polyethylene (PR1050SP, manufactured by Tokyo Ink Co., Ltd.)
MFR: 24g / 10min
Melting point: 105 ° C

From the comparison with Examples 4 and 5, it can be seen that the amount of detected peptide decreases as the amount of the water-permeable layer increases. Therefore, it is considered that a thin water-permeable layer is preferable as the absorption / desorption material from the viewpoint of peptide absorption / desorption. From Examples 8 to 10, it can be seen that the material of the water-permeable layer is preferably PET (polyethylene terephthalate) rather than PP (polypropylene), and PP is more preferred than Nylon (nylon). The details of the effect of the material of the water-permeable layer on the detected amount of peptide are unknown, but the peptide not only absorbs and desorbs to the porous body having mesopores, but also adsorbs to the water-permeable layer and is easy to desorb. There is a possibility that there is a difference. From Example 10, it was shown that EVA is preferable to polyethylene as a binder in terms of the amount of peptide detected.

1, 1A, 1B, 1C Absorption / desorption material 2 Porous body having mesopores 3 Binder 4 Absorption / desorption layer 5 Non-woven fabric 6 Blood 10 Spitz for blood collection
21

Mask

22, 24 Water-permeable layer 23 Mesoporous silica particle layer 25 Laminate 26 Absorption / desorption material

Claims

A biological material selective absorption / desorption material for selectively absorbing / desorbing biological material from a liquid containing biological material,
The selective adsorption / desorption material for biological materials, wherein the adsorption / desorption material is a sheet containing a porous body having mesopores.
2. The selective absorption of biological material according to claim 1, wherein the absorption / desorption material is a laminate in which an absorption / desorption layer including a porous body having mesopores and a water-permeable layer are laminated.・ Desorption material.
3. The biological material selective according to claim 2, wherein the absorbent / desorbing material is a laminate in which a water-permeable layer is laminated on both sides of an absorbent / desorbing layer including a porous body having mesopores. Absorption / desorption material.
The selective adsorption / desorption material for biological materials according to claim 2, wherein the adsorption / desorption material has a porous body having mesopores and a layer containing a binder.
A biological material selective absorption / desorption material for selectively absorbing / desorbing biological material from a liquid containing biological material,
A selective absorption / desorption material for a biological material, wherein the absorption / desorption material is a laminate in which an absorption / desorption layer including a porous body having mesopores and a water-permeable layer are laminated.
A biological material selective absorption / desorption material for selectively absorbing / desorbing biological material from a liquid containing biological material,
The selective adsorption / desorption material for biological materials, wherein the adsorption / desorption material has a layer containing a porous body having mesopores and a binder.
The selective absorption / desorption material for a biological substance according to any one of claims 1 to 6, wherein the mesopore diameter of the porous body having mesopores is 2 nm or more and 20 nm or less.
The selective absorption / desorption material for biological substances according to any one of claims 1 to 7, wherein the porous body having mesopores has an average particle diameter of 50 µm or more and 700 µm or less.
The selective absorption / desorption material for biological materials according to any one of claims 1 to 8, wherein the porous body having mesopores has a specific surface area of 100 m 2 / g or more and 1200 m 2 / g or less.
The selective absorption of a biological substance according to any one of claims 1 to 9, wherein the porous body having mesopores includes one or more selected from the group consisting of mesoporous silica, silica gel, and ion exchange resin.・ Desorption material.
The selective absorption / desorption material for a biological material according to any one of claims 2 to 5 and 7 to 10, wherein the water-permeable layer is a woven fabric or a nonwoven fabric.
The selective absorption / desorption material for a biological material according to any one of claims 2 to 11, wherein the water-permeable layer is subjected to a hydrophilic treatment on an outer surface.
The selective absorption / desorption material for a biological material according to any one of claims 2 to 12, wherein the water-permeable layer is subjected to a hydrophobic treatment on an outer surface.
The selective adsorption / desorption material for biological material according to any one of claims 1 to 13, wherein the biological material is a peptide.
The selective adsorption / desorption material for a biological substance according to any one of claims 4 and 6 to 14, wherein the binder is an ethylene vinyl acetate copolymer.
The biological substance selective according to any one of claims 4 and 6 to 15, wherein the binder is contained in a layer containing a porous body having mesopores and a binder in an amount of 20 wt% to 80 wt%. Absorption / desorption material.
A container capable of holding a liquid containing the biological substance, the container having the absorption / desorption material according to any one of claims 1 to 16 therein.
A biological material purification kit comprising the absorption / desorption material according to any one of claims 1 to 16 and a container capable of holding a liquid containing the biological material.
A method for producing a biological material, comprising the step of bringing the selective absorbing / desorbing material for a biological material according to any one of claims 1 to 16 into contact with a liquid containing the biological material.
A method for separating a biological material, comprising the step of bringing the selective adsorption / desorption material for biological material according to any one of claims 1 to 16 into contact with a liquid containing the biological material.