WO2016011086A3 - Method and kit for protozoa characterization - Google Patents

Method and kit for protozoa characterization Download PDF

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Publication number
WO2016011086A3
WO2016011086A3 PCT/US2015/040456 US2015040456W WO2016011086A3 WO 2016011086 A3 WO2016011086 A3 WO 2016011086A3 US 2015040456 W US2015040456 W US 2015040456W WO 2016011086 A3 WO2016011086 A3 WO 2016011086A3
Authority
WO
WIPO (PCT)
Prior art keywords
protozoa
characterization
kit
visualizing
pathogenic
Prior art date
Application number
PCT/US2015/040456
Other languages
French (fr)
Other versions
WO2016011086A2 (en
Inventor
Stephen E. FRY
Jeremy Ellis
Original Assignee
Fry Laboratories, LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/331,143 external-priority patent/US20140349293A1/en
Application filed by Fry Laboratories, LLC filed Critical Fry Laboratories, LLC
Publication of WO2016011086A2 publication Critical patent/WO2016011086A2/en
Publication of WO2016011086A3 publication Critical patent/WO2016011086A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed are compositions, kits, and methods for detecting, extracting, visualizing, characterizing, and/or identifying one or more protozoa. Various types of polymerase chain reaction techniques in connection with specifically designed oligonucleotide probes can be used to detect a variety of, e.g., pathogenic, protozoa in samples.
PCT/US2015/040456 2014-07-14 2015-07-14 Method and kit for protozoa characterization WO2016011086A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/331,143 2014-07-14
US14/331,143 US20140349293A1 (en) 2011-08-03 2014-07-14 Method and kit for protozoa characterization

Publications (2)

Publication Number Publication Date
WO2016011086A2 WO2016011086A2 (en) 2016-01-21
WO2016011086A3 true WO2016011086A3 (en) 2016-04-21

Family

ID=55079164

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/040456 WO2016011086A2 (en) 2014-07-14 2015-07-14 Method and kit for protozoa characterization

Country Status (1)

Country Link
WO (1) WO2016011086A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017156431A1 (en) * 2016-03-11 2017-09-14 The Joan & Irwin Jacobs Technion-Cornell Institute Systems and methods for characterization of viability and infection risk of microbes in the environment

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005168359A (en) * 2003-12-09 2005-06-30 New Industry Research Organization Method for detecting and detecting laver olpidioid fungus disease pathogenic fungus
WO2006117596A2 (en) * 2004-05-04 2006-11-09 Dako Denmark A/S Nucleic acid probes and nucleic acid analog probes
US20140113301A1 (en) * 2011-05-16 2014-04-24 Universitetet I Oslo Detection of saxitoxin-producing dinoflagellates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005168359A (en) * 2003-12-09 2005-06-30 New Industry Research Organization Method for detecting and detecting laver olpidioid fungus disease pathogenic fungus
WO2006117596A2 (en) * 2004-05-04 2006-11-09 Dako Denmark A/S Nucleic acid probes and nucleic acid analog probes
US20140113301A1 (en) * 2011-05-16 2014-04-24 Universitetet I Oslo Detection of saxitoxin-producing dinoflagellates

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRAMAN, M.: "Protozoal Infectio- The Next Big Discovery? A New Protozoa Appears Linked To Mystery Diseases", DR. FRY INTERVIEW., 12 July 2013 (2013-07-12), pages 1 - 12 *
DATABASE GenBank [O] 9 August 2013 (2013-08-09), "Uncultured eukaryote clone nco77f12c1 18S ribosomal RNA gene, partial sequence", retrieved from NCBI Database accession no. KC673216.1 *
FINDLEY, K. ET AL.: "Topographic Diversity Of Fungal And Bacterial Communities In Human Skin.", NATURE, 2013 *
GUY, R. ET AL.: "Real-Time PCR For Quantification Of Giardia And Cryptosporidium In Environmental Water Samples And Sewage.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 69, no. 9, September 2003 (2003-09-01), pages 5178 - 5185, XP008104236, DOI: doi:10.1128/AEM.69.9.5178-5185.2003 *

Also Published As

Publication number Publication date
WO2016011086A2 (en) 2016-01-21

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