WO2016010131A1 - Prophylactic or therapeutic agent for age-related macular degeneration - Google Patents

Abstract

　The present invention pertains to a prophylactic or therapeutic agent for age-related macular degeneration containing as an active ingredient a compound shown by formula (1), an enantiomer or diastereomer thereof, or a pharmacologically acceptable salt thereof (also referred to as "this compound" below).

Description

Preventive or therapeutic agent for age-related macular degeneration

The present invention relates to 3-aminocyclopentanecarboxamide derivatives, in particular the formula (1):

Or an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof (hereinafter also referred to as “the present compound”) as an active ingredient.

Age-related macular degeneration (hereinafter also referred to as “AMD”) is one of the leading causes of blindness in developed countries, and is mainly seen in elderly people over 50 years old. Age-related macular degeneration is a disease caused by changes associated with aging of the macular, and is roughly classified into wet age-related macular degeneration, atrophic age-related macular degeneration, and early age-related macular degeneration, which are precursor lesions thereof. Here, wet age-related macular degeneration is a disease in which neovascularization occurs in the macular of the elderly from the choroid, causing bleeding or exudative lesions in the retinal pigment epithelium or subretinal, and finally scar tissue. On the other hand, atrophic age-related macular degeneration is a disease accompanied by macular atrophy and drusen accumulation.

The pathological condition underlying wet age-related macular degeneration is choroidal neovascularization, which is thought to develop on the basis of age-related changes such as macular retinal pigment epithelial cells, Bruch's membrane, and choroidal blood vessels. However, the cause and mechanism of choroidal neovascularization has not yet been elucidated, and this is an area where future development is expected. On the other hand, it is known that choroidal neovascularization is not involved in early age-related macular degeneration and atrophic age-related macular degeneration. In atrophic age-related macular degeneration, it is considered that visual acuity decreases due to photoreceptor cell degeneration (cell death) accompanying atrophy, and photoreceptor cell protection is attracting attention as a new treatment method. (Non-Patent Document 1).

Meanwhile, a 3-aminocyclopentanecarboxamide derivative as a chemokine receptor modulator is disclosed, and a plurality of synthesis examples of the derivative are described. Moreover, the antagonistic action with respect to the chemokine receptor CCR2 of the said derivative | guide_body and the renal protective effect in a diabetic nephropathy rat model are verified (patent document 1).

However, 3-aminocyclopentanecarboxamide derivatives, in particular the formula (1):

There is no report which examined the preventive or therapeutic effect with respect to age-related macular degeneration of the compound shown by this, its enantiomer or diastereomer, or its pharmaceutically acceptable salt (this compound). Furthermore, there is no report examining whether this compound has a preventive or therapeutic effect on any type of age-related macular degeneration such as early age-related macular degeneration, atrophic age-related macular degeneration, or wet age-related macular degeneration. .

International Publication 2010/061329 Pamphlet

An object of the present invention is to provide a preventive or therapeutic agent for age-related macular degeneration containing a 3-aminocyclopentanecarboxamide derivative as an active ingredient.

The present inventors conducted extensive research to search for a new preventive or therapeutic agent for age-related macular degeneration containing a 3-aminocyclopentanecarboxamide derivative as an active ingredient. It has been found that it has excellent angiogenesis inhibitory action and vascular permeability enhancement action in ocular tissues, and this compound has an excellent preventive or therapeutic effect on age-related macular degeneration, particularly exudative age-related macular degeneration. As a result, the present invention has been found.

That is, the present invention relates to the following.

The present invention relates to formula (1):

[In Formula (1),
R ¹ represents a hydrogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom;
R ² represents a hydrogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom;
R ³ represents a hydrogen atom, a halogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom;
R ⁴ represents a 6-membered nitrogen-containing aromatic ring group which is unsubstituted or substituted with R ⁵ ;
R ⁵ represents a halogen atom, a lower alkyl group or a lower alkyl group substituted with a halogen atom.
Or an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient is a preventive or therapeutic agent for age-related macular degeneration.

Another aspect of the present invention is as follows:
In the above formula (1),
R ¹ represents a lower alkyl group or a lower alkyl group substituted with a halogen atom,
R ² represents a hydrogen atom or a lower alkyl group,
R ³ represents a lower alkyl group or a lower alkyl group substituted with a halogen atom,
R ⁴ represents the formula (2a) or (2b):

Indicate
R ⁵ represents a lower alkyl group or a lower alkyl group substituted with a halogen atom,
A prophylactic or therapeutic agent for age-related macular degeneration comprising a compound, an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. Here, “•” in the formula (2a) or (2b) represents a bonding point with the nitrogen atom to which R ⁴ is bonded.

In another embodiment of the present invention, the compound represented by the formula (1) is
Formula (1 ′):

Indicated by
A prophylactic or therapeutic agent for age-related macular degeneration comprising a compound or a pharmaceutically acceptable salt thereof as an active ingredient.

Another aspect of the present invention is as follows:
In the above formula (1) or formula (1 ′),
R ¹ represents methyl,
R ² represents a hydrogen atom,
R ³ represents isopropyl,
R ⁴ represents the formula (2b):

Indicate
R ⁵ represents trifluoromethyl,
A prophylactic or therapeutic agent for age-related macular degeneration comprising a compound, an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. Here, · in the formula (2b) represents a point of attachment to the nitrogen atom to which R ⁴ is bonded.

Another aspect of the present invention is to prevent or treat age-related macular degeneration containing a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. An agent for preventing or treating the age-related macular degeneration is exudative age-related macular degeneration.

Another aspect of the present invention is a choroidal neovascularization inhibitor comprising a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. .

Another aspect of the present invention is to prevent or treat age-related macular degeneration containing a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. It is a prophylactic or therapeutic agent, or an inhibitor, in which the dosage form is ophthalmic administration, intravitreal administration, subconjunctival administration, intraconjunctival sac administration, subtenon administration or oral administration.

Another aspect of the present invention is to prevent or treat age-related macular degeneration containing a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. The agent is a prophylactic or therapeutic agent, or an inhibitor whose dosage form is an eye drop, eye ointment, insertion agent, patch, injection, tablet, fine granule or capsule.

Furthermore, the present invention also relates to the following.

Another aspect of the present invention is a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof for use in the prevention or treatment of age-related macular degeneration. .

Another aspect of the present invention provides a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for preventing or treating age-related macular degeneration. Is the use of salt.

Another aspect of the present invention provides age-related macular degeneration comprising a therapeutically effective amount of a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof and an additive. A pharmaceutical composition for the prevention or treatment of

Another aspect of the present invention is a method for preventing or treating age-related macular degeneration, the compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof. Administering an effective amount of a salt.

According to the present invention, age-related macular degeneration, particularly exudative age-related macular, comprising a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. An agent for preventing or treating degeneration can be provided.

Hereinafter, the present invention will be described in detail.

“Halogen atom” means a fluorine atom, a chlorine atom, a bromine atom or an iodine atom.

The “lower alkyl group” refers to a linear or branched alkyl group having 1 to 8 carbon atoms, preferably a linear or branched alkyl group having 1 to 6 carbon atoms. Specific examples include methyl, ethyl, n-propyl, n-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, isopropyl, isobutyl, sec-butyl, tert-butyl, isopentyl and the like.

The “lower alkyl group substituted with a halogen atom” refers to a lower alkyl group substituted with one or more (eg, 2 or 3) halogen atoms, and the lower alkyl substituted with 3 halogen atoms Groups are preferred. Specific examples include trifluoromethyl and the like. When a plurality of halogen atoms are present, these halogen atoms may be the same or different.

The “6-membered nitrogen-containing aromatic ring group” means a monocyclic or polycyclic aromatic ring having 6 ring atoms, at least one of which is a nitrogen atom, An aromatic ring containing 1 or 2 nitrogen atoms is preferred. Specific examples include pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl and the like.

The compound contained in the preventive or therapeutic agent for age-related macular degeneration according to the present invention can be produced according to a usual method in the field of synthetic organic chemistry. For example, it can be produced according to the method described in International Publication No. 2010/061329 pamphlet. In addition, geometric isomers (cis-trans isomers), optical isomers (enantiomers, diastereomers) or tautomers of the compound are isolated and purified by usual methods such as column chromatography and HPLC. You can also

The compound contained in the preventive or therapeutic agent for age-related macular degeneration according to the present invention has the formula (1):

Or an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof.

When a geometric isomer (cis-trans isomer), optical isomer (enantiomer, diastereomer) or tautomer exists in the compound represented by the above formula (1), these are also represented by the formula (1) It is included in the range of the compound represented by. In addition, the compound represented by the formula (1) is 1 or 2 selected from the group of geometric isomers (cis-trans isomers), optical isomers (enantiomers, diastereomers) and tautomers. It may be a mixture of the above isomers.

In the above formula (1), R ¹ represents a hydrogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom.

In the above formula (1), R ² represents a hydrogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom.

In the above formula (1), R ³ represents a hydrogen atom, a halogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom.

In the above formula (1), R ⁴ represents a 6-membered nitrogen-containing aromatic ring group which is unsubstituted or substituted with R ⁵ .

In the above formula (1), R ⁵ represents a halogen atom, a lower alkyl group or a lower alkyl group substituted with a halogen atom.

Preferred examples of each substituent are shown below.

In the above formula (1), R ¹ is preferably a lower alkyl group or a lower alkyl group substituted with a halogen atom, particularly preferably a lower alkyl group, and most preferably methyl.

In the above formula (1), R ² is preferably a hydrogen atom or a lower alkyl group, and particularly preferably a hydrogen atom.

In the above formula (1), R ³ is preferably a lower alkyl group or a lower alkyl group substituted with a halogen atom, particularly preferably a lower alkyl group, and most preferably isopropyl.

In the above formula (1), R ⁴ is preferably formula (2a) or (2b), particularly preferably formula (2b).

In the above formula (1), R ⁵ is preferably a lower alkyl group or a lower alkyl group substituted with a halogen atom, particularly preferably a lower alkyl group substituted with a halogen atom, most preferably trifluoromethyl. It is.

The above formula (1) is preferably the formula (1 ′):

It is.

Specific examples of the compound represented by the above formula (1) or formula (1 ′) include the formula (1a):

1,5-anhydro-2,3-dideoxy-3-{[(1R, 3S) -3-isopropyl-3-({(1S, 4S) -5- [5- (trifluoromethyl)] Pyridazin-3-yl] -2,5-diazabicyclo [2,2,1] hept-2-yl} carbonyl) cyclopentyl] amino} -4-O-methyl-D-erythro-pentitol (hereinafter referred to as “Compound 1a Also enantiomers and diastereomers, and mixtures thereof (eg, racemic mixtures, diastereo mixtures, etc.).

Specific examples of the compound represented by the formula (1) or the formula (1 ′) include a formula (1b):

1,5-anhydro-2,3-dideoxy-3-{[(1R, 3S) -3-isopropyl-3-({(1S, 4S) -5- [6- (trifluoromethyl)] Pyrimidin-4-yl] -2,5-diazabicyclo [2,2,1] hept-2-yl} carbonyl) cyclopentyl] amino} -4-O-methyl-D-erythro-pentitol (hereinafter referred to as “Compound 1b Also enantiomers and diastereomers, and mixtures thereof (eg, racemic mixtures, diastereo mixtures, etc.).

The “pharmaceutically acceptable salt” of the compound represented by the above formula (1), (1 ′), (1a) or (1b) includes, for example, a salt with an inorganic acid or a salt with an organic acid. It is done. Examples of the inorganic acid include hydrochloric acid, hydrobromic acid, hydroiodic acid, nitric acid, sulfuric acid, phosphoric acid and the like. Examples of organic acids include acetic acid, fumaric acid, maleic acid, succinic acid, citric acid, tartaric acid, adipic acid, gluconic acid, glucoheptonic acid, glucuronic acid, terephthalic acid, methanesulfonic acid, lactic acid, hippuric acid, 1, 2 -Ethanedisulfonic acid, isethionic acid, lactobionic acid, oleic acid, pamoic acid, polygalacturonic acid, stearic acid, tannic acid, trifluoromethanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, lauryl sulfate, methyl sulfate, naphthalene Examples include sulfonic acid and sulfosalicylic acid.

The compound represented by the above formula (1), (1 '), (1a) or (1b) may be in the form of a hydrate or a solvate.

When a crystal polymorph and a crystal polymorph group (crystal polymorph system) exist in the compound represented by the above formula (1), (1 ′), (1a) or (1b), those crystal polymorphs and Crystal polymorph groups (crystal polymorph systems) are also included within the scope of the compounds of the present invention. Here, the crystal polymorph group (crystal polymorph system) refers to various crystal forms depending on conditions and states (including the formulated state in this state) such as production, crystallization, and storage of these crystals. It means the crystal form and the whole process at each stage when changing.

In the present invention, age-related macular degeneration refers to wet age-related macular degeneration, atrophic age-related macular degeneration, and early age-related macular degeneration as a precursor lesion thereof as described above.

When this compound is used for the prevention or treatment of age-related macular degeneration, it can be administered to patients orally or parenterally. As the dosage form, oral administration, topical administration to the eye (instillation administration) , Intraconjunctival sac administration, intravitreal administration, subconjunctival administration, subtenon administration, etc.), intravenous administration, transdermal administration, and the like. As a preferable dosage form used when the compound is locally administered to the eye, an eye drop or an eye ointment is used, or an injection, particularly a subconjunctival agent, a tenon sac administration agent or an intravitreal administration agent is used. Used. A preparation containing the present compound as an active ingredient is formulated into a dosage form suitable for administration together with a pharmaceutically acceptable additive, if necessary. Examples of dosage forms suitable for oral administration include tablets, capsules, granules, powders, and the like. Examples of dosage forms suitable for parenteral administration include injections, eye drops, eye ointments, and patches. Agents, gels, intercalating agents and the like. These can be prepared using conventional techniques widely used in the field. Furthermore, in order to utilize the sustained action of the therapeutic effect of the present invention more effectively, it may be a DDS preparation such as an intraocular implant preparation or a microsphere.

For example, a tablet can be prepared by appropriately selecting an excipient, a disintegrant, a binder, a lubricant, a coating agent, a corrigent and the like. Examples of the excipient include lactose, glucose, D-mannitol, anhydrous calcium hydrogen phosphate, starch, and sucrose. Examples of the disintegrant include carboxymethylcellulose, carboxymethylcellulose calcium, croscarmellose sodium, crospovidone, starch, partially pregelatinized starch, and low-substituted hydroxypropylcellulose. Examples of the binder include hydroxypropyl cellulose, ethyl cellulose, gum arabic, starch, partially pregelatinized starch, polyvinyl pyrrolidone, and polyvinyl alcohol. Examples of the lubricant include magnesium stearate, calcium stearate, talc, hydrous silicon dioxide, and hardened oil. Examples of the coating agent include purified sucrose, hydroxypropylmethylcellulose, hydroxypropylcellulose, methylcellulose, ethylcellulose, polyvinylpyrrolidone and the like. Examples of the corrigent include citric acid, aspartame, ascorbic acid, menthol and the like.

For example, injections can be prepared by selecting and using isotonic agents, buffering agents, surfactants, thickeners and the like as necessary. As an isotonizing agent, sodium chloride etc. are mentioned, for example. Examples of the buffering agent include sodium phosphate. Examples of the surfactant include polyoxyethylene sorbitan monooleate. Examples of the thickener include methyl cellulose.

For example, the eye drops can be selected and used as necessary from isotonic agents, buffering agents, surfactants, stabilizers, preservatives, etc., and the pH is acceptable for ophthalmic preparations. Although it may be within the range, it is usually preferably within the range of 4-8. Examples of the isotonic agent include sodium chloride and concentrated glycerin. Examples of the buffering agent include sodium phosphate and sodium acetate. Examples of the surfactant include polyoxyethylene sorbitan monooleate, polyoxyl 40 stearate, polyoxyethylene hydrogenated castor oil, and the like. Examples of the stabilizer include sodium citrate and sodium edetate. Examples of the preservative include benzalkonium chloride and paraben.

For example, the eye ointment can be prepared using a commonly used base such as white petrolatum, liquid paraffin and the like.

For example, the intercalating agent is prepared by crushing and mixing a biodegradable polymer, for example, a biodegradable polymer such as hydroxypropylcellulose, hydroxypropylmethylcellulose, carboxyvinyl polymer, polyacrylic acid and the like, and compressing the powder. And an excipient, a binder, a stabilizer, and a pH adjuster can be used as necessary.

For example, an intraocular implant preparation can be prepared using a biodegradable polymer, for example, a biodegradable polymer such as polylactic acid, polyglycolic acid, lactic acid / glycolic acid copolymer, and hydroxypropylcellulose.

The dose of this compound can be appropriately changed according to the dosage form, the severity of the patient's symptoms, age, weight, eye volume, doctor's judgment, etc. 0.01 to 10000 mg per day, preferably 0.1 to 5000 mg, more preferably 0.5 to 2500 mg can be administered in one or several divided doses. For adults, 0.0001 to 2000 mg can be administered once or in several divided doses. In the case of eye drops or intercalating agents, 0.000001 to 10% (w / v), preferably 0.00001 to 1% (w / v), more preferably 0.0001 to 0.1% ( The active ingredient concentration of w / v) can be administered once or several times a day. Furthermore, in the case of a patch, a patch containing 0.0001 to 2000 mg can be applied to an adult. In the case of an intraocular implant preparation, 0.0001 to 2000 mg is included for an adult. An intraocular implant formulation can be implanted in the eye.

The results of pharmacological tests and formulation examples are shown below, but these examples are for better understanding of the present invention and do not limit the scope of the present invention.

[Pharmacological test 1]
The usefulness of this compound was evaluated using a laser-induced rat choroidal neovascularization model (Invest. Ophthalmol. Vis. Sci., 40 (2), 459-466 (1999)).

(Production method of krypton laser-induced rat choroidal neovascularization model animal)
Rats were anesthetized by intramuscular administration of 1 mL / kg of a mixed solution (7: 1) of 5% (W / V) ketamine hydrochloride injection and 2% xylazine hydrochloride injection, and 0.5% (W / V) Tropicamide-0.5% phenylephrine hydrochloride ophthalmic solution was instilled to make mydriasis, and then photocoagulated with a krypton laser photocoagulator. Photocoagulation was performed in the posterior region of the fundus, avoiding thick retinal blood vessels, and focusing on the deep retina at 8 spots per eye (coagulation conditions: spot size 100 μm, output 100 mW, coagulation time 0.1 seconds). . After photocoagulation, fundus photography was performed to confirm the laser irradiation site.

(Test compound)
In this pharmacological test, the compound 1b synthesized according to the synthesis method described in International Publication No. 2010/061329 pamphlet was used as the present compound.

(Drug administration method)
Compound 1b is mixed with 1% (W / V) methylcellulose solution (prepared by dissolving methylcellulose in purified water) to 0.2 and 2 mg / mL, and compound 1b is administered at doses of 1 and 10 mg / kg. The liquid was orally administered twice a day for 7 days including the operation day from the photocoagulation operation day. A 1% (W / V) methylcellulose solution was similarly administered to the base administration group.

(Evaluation methods)
On day 7 after photocoagulation, the rats were given general anesthesia by intramuscular administration of 1 mL / kg of a mixed solution (7: 1) of 5% (W / V) ketamine hydrochloride injection and 2% xylazine hydrochloride injection. After 5% (W / V) tropicamide-0.5% phenylephrine hydrochloride ophthalmic solution was instilled to make mydriatic, 0.1 mL of 10% fluorescein solution was injected from the penile vein, and fluorescence fundus imaging was performed. In the fluorescence fundus angiography, a spot where no fluorescence leak was observed was judged negative (no angiogenesis), and a spot where fluorescence leak was found was judged positive. In addition, a photocoagulation site in which slight fluorescence leakage was observed was determined to be positive (with angiogenesis) when two sites were present. Thereafter, the rate of choroidal neovascularization (%) was calculated from the number of positive spots with respect to 8 spots irradiated with laser according to Formula 1, and the rate of inhibition (%) of the evaluation drug was calculated according to Formula 2. The results for compound 1b are shown in Table 1. The number of cases in each administration group is 6 to 8.

[Formula 1]:
Incidence rate of choroidal neovascularization (%) = (number of positive spots / total number of photocoagulation sites) × 100

[Formula 2]:
Inhibition rate (%) = {(A ₀ −A _X ) / A ₀ } × 100
A ₀ : incidence rate of choroidal neovascularization in base administration group A _X : incidence rate of choroidal neovascularization in drug administration group

As is clear from Table 1, Compound 1b was shown to inhibit choroidal neovascularization in a laser-induced rat choroidal neovascularization model animal. From the above results, it was shown that the present compound has an excellent angiogenesis inhibitory action in the choroid and has an excellent preventive or therapeutic effect on age-related macular degeneration, particularly wet age-related macular degeneration.

[Formulation example]
The pharmaceutical agent of the present invention will be described more specifically with formulation examples, but the present invention is not limited to these formulation examples.

Formulation Example 1 10 mg of this compound in 100 mL of eye drops
Sodium chloride 900mg
Polysorbate 80 Appropriate amount Disodium hydrogen phosphate Appropriate amount Sodium dihydrogen phosphate Appropriate amount Sterilized purified water Appropriate amount

Add this compound and other components to sterilized purified water and mix them thoroughly to prepare eye drops. By changing the amount of the compound added, eye drops having a concentration of 0.05% (w / v) to 1% (w / v) can be prepared.

Formulation Example 2 0.3 g of this compound in 100 g of eye ointment
Liquid paraffin 10.0g
White petrolatum

An eye ointment is prepared by adding this compound to uniformly melted white petrolatum and liquid paraffin, mixing them well, and gradually cooling. By changing the amount of this compound added, an eye ointment having a concentration of 0.05% (w / v) to 1% (w / w) can be prepared.

Formulation Example 3 1 mg of this compound in 100 mg of tablets
Lactose 66.4mg
Corn starch 20mg
Carboxymethylcellulose calcium 6mg
Hydroxypropylcellulose 6mg
Magnesium stearate 0.6mg

This compound and lactose are mixed in a blender, carboxymethylcellulose calcium and hydroxypropylcellulose are added to the mixture and granulated. The resulting granules are dried and sized, and magnesium stearate is added to the sized granules. Mix and compress with a tableting machine. In addition, tablets with a content of the present compound in 100 mg of 0.1 mg to 50 mg can be prepared by appropriately changing the addition amount of the present compound, carboxymethylcellulose calcium and hydroxypropylcellulose.

Formulation Example 4 10 mg of this compound in 10 mL of injection or intravitreal administration
Sodium chloride 90mg
Polysorbate 80 Appropriate amount Sterilized purified water Appropriate amount

Add the compound and other components to sterilized purified water, mix well and dissolve or suspend to prepare an injection. By appropriately changing the addition amount of the present compound and the other components described above, an injection with a content of the present compound in 10 mL of 2 mg to 200 mg can be prepared. The injection prepared as described above can be administered as an injection for intraocular administration, for example, an intravitreal administration.

Claims (8)

1. Formula (1):
   

   

   
   [In Formula (1),
   R ¹ represents a hydrogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom;
   R ² represents a hydrogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom;
   R ³ represents a hydrogen atom, a halogen atom, a lower alkyl group, or a lower alkyl group substituted with a halogen atom;
   R ⁴ represents a 6-membered nitrogen-containing aromatic ring group which is unsubstituted or substituted with R ⁵ ;
   R ⁵ represents a halogen atom, a lower alkyl group or a lower alkyl group substituted with a halogen atom.
   A prophylactic or therapeutic agent for age-related macular degeneration comprising the compound represented by the above, an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient.
2. In formula (1),
   R ¹ represents a lower alkyl group or a lower alkyl group substituted with a halogen atom,
   R ² represents a hydrogen atom or a lower alkyl group,
   R ³ represents a lower alkyl group or a lower alkyl group substituted with a halogen atom,
   R ⁴ represents the formula (2a) or (2b):
   

   

   Indicate
   R ⁵ represents a lower alkyl group or a lower alkyl group substituted with a halogen atom,
   The preventive or therapeutic agent according to claim 1.
3. Formula (1) becomes Formula (1 ′):
   

   

   
   The preventive or therapeutic agent of Claim 1 or 2 which shows this.
4. In formula (1) or formula (1 ′),
   R ¹ represents methyl,
   R ² represents a hydrogen atom,
   R ³ represents isopropyl,
   R ⁴ represents the formula (2b):
   

   

   
   Indicate
   R ⁵ represents trifluoromethyl,
   The preventive or therapeutic agent according to any one of claims 1 to 3.
5. The preventive or therapeutic agent according to any one of claims 1 to 4, wherein the age-related macular degeneration is exudative age-related macular degeneration.
6. A choroidal neovascularization inhibitor comprising a compound represented by the above formula (1), an enantiomer or diastereomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient.
7. The preventive or therapeutic agent or inhibitor according to any one of claims 1 to 6, wherein the administration form is ophthalmic administration, intravitreal administration, subconjunctival administration, intraconjunctival sac administration, subtenon sac administration or oral administration.
8. The prophylactic or therapeutic agent or inhibition according to any one of claims 1 to 7, wherein the dosage form is an eye drop, an eye ointment, an insertion agent, a patch, an injection, a tablet, a fine granule, or a capsule. Agent.