WO2016004830A1 - 一种电动剥卵装置及其剥卵方法 - Google Patents

一种电动剥卵装置及其剥卵方法 Download PDF

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WO2016004830A1
WO2016004830A1 PCT/CN2015/083133 CN2015083133W WO2016004830A1 WO 2016004830 A1 WO2016004830 A1 WO 2016004830A1 CN 2015083133 W CN2015083133 W CN 2015083133W WO 2016004830 A1 WO2016004830 A1 WO 2016004830A1
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egg
module
tube
micro
electric
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PCT/CN2015/083133
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English (en)
French (fr)
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彭建军
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彭建军
长沙傲图生物科技有限公司
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Priority to US15/323,719 priority Critical patent/US10184107B2/en
Priority to EP15818614.8A priority patent/EP3165187B1/en
Publication of WO2016004830A1 publication Critical patent/WO2016004830A1/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0227—Details of motor drive means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/09—Means for pre-treatment of biological substances by enzymatic treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/42—Gynaecological or obstetrical instruments or methods
    • A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
    • A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0237—Details of electronic control, e.g. relating to user interface
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation

Definitions

  • the invention relates to a reproductive laboratory device, in particular to an electric egg stripping device and a method for removing the same, and belongs to the technical field of laboratory microscopic operation.
  • Peeling is an important part of the daily work of the reproductive laboratory, including: stripping the granulosa cells of the enzyme-pre-digested egg-crown complex to assess oocyte maturity and facilitate micromanipulation, such as intracytoplasmic sperm injection ( (Intracytoplasmic sperm injection, ICSI for short), oocytes need to be stripped of granulosa cells before freezing; in vitro fertilization (IVF), such as microdropping IVF, IVF, need to remove the oocytes of the oocyte Cells in order to assess fertilization. Exfoliation plays an important role in the above operations, directly affecting the effects of subsequent operations.
  • the traditional oviduct tube includes an egg stripping needle and an egg stripping tube, respectively, and a corresponding latex tip is respectively formed to form an egg stripping ovipositor and a pasteurized tube ovipositor.
  • the traditional method of spawning is mainly to use the finger suction force repeatedly to press the latex suction head to make the hyalinase pre-digested egg crown mound complex or the IVF complex after digesting the sperm in IVF.
  • the ovule tube is stripped of the granulosa cells, or the oocytes adhered to the egg-crown complex at the bottom of the culture dish after the IVF droplets are separated from the complex by suction, and then repeatedly stripped by the suction.
  • Granular cells There is also a straw-type oviduct which is formed by a straw connecting nozzle of a spawning tube, and the nozzle is placed in the oral cavity, and the granule cells are removed by the oral suction to enter and exit the stripping tube mouth.
  • oral balloon ovipositor (patent number: CN200720157432.9) is an oral balloon ovipositor that peels off the egg-crown complex around the oocyte. It includes a micropipette, at The rear end of the micropipette is sleeved with a hose, and a soft balloon is attached to the end of the hose. During operation, the balloon is placed between the upper and lower teeth of the mouth, and the balloon is controlled by biting the teeth to provide a blowing force, so that the oocytes repeatedly and gently enter and exit the tip of the micropipette to remove most of the granular cells surrounding the oocyte. the goal of.
  • the invention replaces the mouthpiece of the mouth straw with a balloon, and squeezes the balloon through the tooth to achieve the suction effect. Compared with the mouth pipette method, the method can avoid the risk of oral exhalation polluting oocytes.
  • the patent "oval needle” (Patent No.: CN201220219074.0) provides an egg stripping needle suitable for all size oocytes.
  • the invention is for use in a hollow egg-peeling needle for detaching granulosa cells of granulosa cells surrounding an oocyte.
  • the invention has only been modified for the structure of the egg-peeling needle, and the method of spawning is the same as the conventional method.
  • the patented "a new IVF egg stripping operator" provides an egg peeling device consisting of a hand-held pressing device and a suction oocyte device, and the hand-held pressing device is composed of a pressure bar cap, a cap, and a back shell.
  • the spring, the pressure bar and the front shell are formed, and the oocyte device is composed of an air bag, a PS tube, a needle seat and an oviduct tube, and the air bag, the PS tube, the needle seat and the oviduct are sequentially mounted on the back shell from top to bottom.
  • Middle and lower parts; the middle of the front case is equipped with a push button connected to the slider.
  • the invention is significantly more maneuverable than conventional latex tips.
  • the control is inaccurate and there are multiple risks.
  • the latex tip, the mouth pipette, the oral balloon, and the balloon of the elastic operating element of the omelette are controlled by the muscle strength of the operator.
  • the elasticity of the material has the ability to release the compression deformation and restore the original shape, which is the power source for the suction of such manual spawning device.
  • the squeezing force applied by the operator to the original elastic operating element is a source of power for such manual spawning to generate a blow.
  • the function of the mouth is similar to the elastic operating element of the above-mentioned omelette, except that both blowing and suction are processes in which the operator actively applies force.
  • the suction and blow force generation and conversion processes are always accompanied by a counterbalance between the pressing force and the restoring force of the elastic element.
  • the egg-crown complex is slightly larger than the inner diameter of the stripping tube, and blockage occurs when the tube is peeled off, which is manifested by the egg-thick complex.
  • the tube nozzle speed is slowed down.
  • the operator adjusts the suction or blowing force, especially after increasing the blowing force, once the egg-crown complex passes through the stripping tube, the operator can not brake in time, and the two processes of suction and blowing are unbalanced. Inconsistent volume changes, the volume change caused by the blow is greater than the volume change caused by the suction, resulting in a blow out String bubbles.
  • the egg-crown complex or oocyte is adsorbed by bubbles to its edge and floats on the surface of the medium droplet with the bubble.
  • the spawning operation interface and the bubble exist on different focal planes, and the egg-crown complex or oocyte adsorbed by the bubble can also be located at any position on the surface of the bubble, and the search for the egg-crown complex or oocyte at the edge of the bubble needs constant Adjusting the focal length of the microscope, resulting in prolonged whole egg stripping operation, not only increases the risk of damaging the oocyte due to in vitro operation time, but also poses a significant risk of losing oocytes.
  • the operator usually takes a few microliters of the medium in the tipping tube and stays at the tip of the oviduct, in order to compensate for the inconsistent volume of the suction tube, so that the length of the liquid column in the spawning tube is far beyond the culture.
  • the height of the liquid in the dish In order to avoid the generation of air bubbles as much as possible, the operator usually takes a few microliters of the medium in the tipping tube and stays at the tip of the oviduct, in order to compensate for the inconsistent volume of the suction tube, so that the length of the liquid column in the spawning tube is far beyond the culture. The height of the liquid in the dish.
  • the existing method of spawning is inaccurately controlled so that the oocyte or zygote is at risk of being placed in temperature fluctuations. Temperature is one of the most important factors affecting the quality of oocytes, embryos and embryos.
  • the basal body temperature of human is 37 ° C
  • the temperature in the environment where the oocyte or zygote is located is 37 ° C.
  • the spawning operation is carried out in a ventilated clean bench.
  • the temperature of the clean bench is about 37 ° C
  • the room temperature is about 25 ° C
  • the temperature of the medium in the petri dish where the egg-crown complex is located. It is close to the ultra-clean surface temperature.
  • the tip of the oviduct is very small.
  • the medium level in the tip of the oviduct is much higher than the level of the dish.
  • the tip of the medium that is not in the dish has only about 0.1 ⁇ L of the medium.
  • the temperature is close to the medium in the dish and the remainder is exposed outside the medium in the dish.
  • the oviduct has two distinct regions of temperature: the first region, the tip of the culture medium that is not in the culture medium, the temperature and the culture medium are close to each other; and the second region, which is not included in the culture medium. In part, the temperature is close to room temperature.
  • the heat is quickly taken away by the wind through the wall of the spawning tube, and the oocyte or zygote moves back and forth in an environment with a temperature difference of a few degrees, and there is a temperature due to the temperature. Fluctuations lead to the risk of damaging oocytes or zygotes.
  • the effects of hypothermia on oocytes and zygotes include reducing their metabolic levels and altering organelle morphology and function. For example, at a temperature of 33 ° C, the spindle will begin to depolymerize within 5 minutes and completely disappear within 10 minutes.
  • the spindle can be fully recovered in 10 minutes. If the temperature drops to 28 ° C, the spindle depolymerization speed is increased, the time to complete disappearing is shortened, and the recovery time is longer after heating. If the spindle is disintegrated and heated to 37 ° C in an environment of 25 ° C, it is difficult to recover within 20 minutes. During the recovery period, if there is a change in cell cycle, the number of chromosomes will be abnormal. It is generally believed that the temperature of human oocytes and zygotes in the in vitro operation must be kept within a relatively constant range, such as 35 ⁇ 37 ° C, to minimize the temperature wave during the operation. move.
  • the liquid column that needs to be inhaled into the oviduct is placed below the droplets of the dish.
  • the height of the culture medium of the culture medium of the egg-crown complex is about 2.5 mm, and the angle between the peeling tube and the bottom of the culture dish is 30 degrees.
  • the length of the liquid column in the peeling tube is less than 5 mm, so as to ensure the culture in the peeling tube.
  • the base liquid level is below the liquid level of the dish.
  • the tip of the oviduct is very small, the inner diameter of the tip is generally 150 ⁇ m, and the height of the medium in the tip is 5 mm.
  • the volume of the medium is about 0.1 ⁇ L. In such a small volume of medium, the elastic operating element of the ovipositor is controlled by the muscle strength of the operator to allow the egg-crown complex to enter and exit the oviduct, and it is almost impossible to adjust the speed without generating bubbles.
  • the operation is subjective and cannot be standardized.
  • the egg peeling operation requires gentle blowing, but the “soft blowing” cannot be clearly defined.
  • Different operators have different subjective feelings for “soft blowing”, which is characterized by pressing the latex tip, spring bar, and call when using the above traditional spawning device.
  • the flow rate of the liquid is positively related to the pressure.
  • the liquid flow rate can be adjusted to adjust the nozzle pressure, thereby adjusting the suction and blowing force of the effect of the egg-crown composite. That is to say, the frequency of blowing and sucking increases, and the suction and blowing force of the egg-crown complex are increased. Further, the force of peeling off the granulosa cells is correspondingly increased, and the peeling speed is accelerated. Therefore, the rate of spalling can be adjusted by adjusting the blowing frequency.
  • the existing ovipositor adjusts the suction and blowing force of the egg-crown complex by pressing the latex tip, the spring bar, the exhalation/inhalation, the depth and the speed of the tooth occlusion.
  • several types of blowing speeds are generally used, and correspondingly there are several blowing forces and suction forces.
  • Different blow forces and suctions and their duration, especially the excessively intense blow and suction and their duration, have different potential negative effects on the oocyte, and too much blownness can cause the oocyte to rupture and collapse.
  • the work intensity is large.
  • the existing method of spawning must correspond to one press/release of the elastic element or the mouth blowing/suction action each time the sucking egg crown compound complex is blown.
  • Existing ovipositors are still used with one hand It is a two-hand operation, or oral control. In order to obtain the best control effect, the operator maintains the antagonistic balance that is always accompanied by the suction and the blow conversion process. The finger or the oral muscles are always in a state of tension, causing physical fatigue of the operator. Because the traditional ovipositor has the time to prolong the risk of damaging the oocyte, the risk of temperature fluctuations leading to the damage of the oocyte and the significant risk of losing the oocyte, resulting in an increase in the operator's psychological burden.
  • an object of the present invention is to provide an electric egg plucking device which is excellent in accuracy, high in standardization, strong in comfort, and high in automation.
  • Another object of the present invention is to provide a method of spawning using the above-described electric spawning device.
  • an electric egg-peeling device comprising an egg-removing tube, a control handle, a power module, a control module, a power module, a memory and a tip mounted in the control handle
  • one end of the control handle is provided with a connection port, the spawning tube is connected to the connection port
  • the power module comprises a micro-cylinder module, a transmission mechanism and a stepping motor
  • the control module comprises a stepping motor a stepper motor driver, a button module, a display module, a power module, and a memory are respectively connected to the single chip microcomputer
  • the stepping motor driver is connected to the stepping motor
  • the stepping motor Connected to the transmission mechanism, the transmission mechanism is connected to a micro push rod; the other end of the micro push rod is placed in the micro cylinder tube, and the micro cylinder tube is in communication with the spawning tube sealing tube;
  • the tip is in communication with the oviduct.
  • the electric egg-peeling device of the present invention is further configured such that the spawning tube is specifically an egg-peeling tip, an egg-peeling needle or an egg-exfoliating tube.
  • the electric egg plucking device of the present invention is further provided with a start button, an accelerator button and a deceleration button on the control handle, and the start button, the accelerator button and the deceleration button are electrically connected to the button module respectively.
  • the electric egg plucking device of the present invention is further configured to: a power module compartment and a main compartment are disposed in the control handle; the power module is housed in the power module compartment; the control module and the power module are housed in the main compartment; A display screen is also embedded on the control handle.
  • the electric egg-peeling device of the present invention is further configured such that a central hole is formed in the center of the connecting port, and a port end of the central hole is reduced to form a limiting ring; the central hole receives a sealing tube, and the limiting ring Restricted in the central hole, the outer wall of the sealing tube is in close contact with the inner wall of the central hole; the opening of one end of the sealing tube has an interface that forms a plug-in tight connection with the spawning tube; The other end opening of the tube has an interface that forms a tight connection with the communicating microcylinder, an interface that forms a tight connection with the communication tip, and an interface that forms a tight connection with the connection pressure sensor.
  • the electric egg-peeling device of the present invention is further configured to: the micro-cylinder module includes a micro-cylinder, a sealing tube, a pressing member, and a compression nut; wherein the micro-push rod and the micro-cylinder are connected; the sealing a tube is received in the micro cylinder, the pressing member is in close contact with the sealing tube, and the pressing nut is screwed to the micro cylinder, a part of which is located in the pressing nut, and another A portion extends out of the circular opening of the compression nut.
  • the electric egg-peeling device of the present invention is further configured to: the transmission mechanism includes a screw rod and a nut-slider; wherein the screw rod is connected to the stepping motor; one end of the micro-push rod is fixed to the nut- On the slider.
  • the electric egg-peeling device of the present invention is further configured to: the transmission mechanism includes a spur gear and a straight rack-slider; wherein the spur gear is assembled on the stepping motor; one end of the micro-push rod is fixed at The straight rack - on the slider.
  • the electric egg plucking device of the present invention is further configured to: further, a voice module is provided, and the voice module is connected to the single chip microcomputer.
  • the electric egg plucking device of the present invention is further configured to further include a control box that is coupled to the steering handle by a cable.
  • the electric egg-peeling device of the present invention is further configured to: provide a display screen on the control box; or provide a display screen on the control handle and the control box respectively.
  • the electric egg-peeling device of the present invention is further configured to further include a pressure sensor, the pressure sensor also being coupled to the single-chip microcomputer, and the pressure detecting element of the pressure sensor is in communication with the spawning tube through a pipe.
  • the technical solution adopted by the present invention is: a method for spawning, comprising the steps of: transferring the egg-crown complex into the hyaluronidase solution by using a non-drawn pasteurized tube, continuously Blow several times to remove most of the mucus mass, then transfer the pre-digested egg-crown complex to the operation
  • the stepping motor provides the suction power to repeatedly advance the pre-digested egg-crown complex into and out of the mouth of the spawning tube until the granular cells are removed to meet the operational requirements.
  • the method for spawning of the present invention further comprises: the stepping motor is controlled by a single chip microcomputer, and the method includes at least three speed regulation modes: alternately using a pre-stored program speed regulation, manually increasing or decreasing the single amplitude on the basis of the operating program blowing frequency. It can be automatically accelerated to a certain set blowing frequency high value based on the programmed frequency value and the running program blowing frequency, and the original blowing frequency can be restored after the set time.
  • a method for spawning comprising the steps of: suspending the egg-crown complex medium after IVF in a test tube by using an undrawn batten tube; The liquid is transferred to the empty culture dish, and the suction motor is provided by the stepping motor to make the egg-crown complex repeatedly enter and exit the mouth of the spawning tube until the degree of removal of the granular cells reaches the operation requirement; the micro-drop method is used to peel the eggs after IVF.
  • the motor provides the suction and suction power to repeatedly suck the oocytes which are attached to the bottom of the culture dish with the granule cells, so as to fall off from the bottom of the dish, and then repeatedly suck until the granule cells are removed to meet the operation requirements.
  • the method for spawning of the present invention may further be: the stepping motor is controlled by a single chip microcomputer, and at least comprises three speed regulation modes: alternately using a pre-stored program speed regulation, manually increasing or decreasing a single time based on a running program blowing frequency The amplitude can be automatically accelerated to a certain set blowing frequency high value based on the programmed frequency value and the running program blowing frequency, and the original blowing frequency is restored after the set time.
  • the present invention has the following beneficial effects:
  • Positioning is accurate, safe and effective, and the egg-peking operation does not produce bubbles. It not only shortens the operation time but also avoids the loss of oocytes.
  • the control is precise, and the temperature of the medium in which the oocyte is located is greatly fluctuated, which can minimize the temperature fluctuation on the oocyte. The damage is positive for improving pregnancy outcomes.
  • the process of stripping operation has quantitative indicators to reduce the influence of subjective factors.
  • the oviduct operation record can be saved and inquired, which solves the problem that the traditional egg-peking technique cannot be compared between different operators and the same operator cannot review and analyze the previous operation. It is of great significance to standardize the operation of spawning.
  • Figure 1 is a schematic view showing the principle of the electric egg plucking device of the present invention
  • Figure 2-1 is a schematic view of a stripping tip
  • Figure 2-2 is a schematic view of the egg stripping needle
  • Figure 2-3 is a schematic view of a stripping tube
  • Figure 3-1-1 is an exploded view of the integrated electric peeling device control handle of the present invention.
  • Figure 3-1-2 is a partial view of the connection port and the sealing tube of Figure 3-1-1;
  • 3-2 is a perspective view of the split type electric ovipositor control handle and control box of the present invention.
  • Figure 4-1-1 is an exploded view of the control handle of the integrated electric ovipositor of the present invention.
  • Figure 4-1-2 is a partial view of the connection port and the sealing tube of Figure 4-1-1;
  • 4-2 is a perspective view of the control handle and control box of the split type electric egg stripping device of the present invention.
  • Figure 5-1-1 is an exploded view of the microcylinder module and the microcylinder module of the electric egg plucking device of the present invention
  • Figure 5-1-2 is an exploded view of another form of the micro-cylinder module and the micro-cylinder module of the electric egg-peeling device of the present invention.
  • 5-2 is a perspective view of a power module and a power module of the electric egg plucking device of the present invention
  • 5-3 is a perspective view showing another form of a power module and a power module of the electric egg plucking device of the present invention.
  • Figure 6-1 is an integrated electric egg stripping device for connecting the egg stripping tip of the present invention
  • Figure 6-2 is an integrated electric egg-peeling device for connecting the stripping needle according to the present invention.
  • Figure 6-3 is an integrated electric egg-peeling device for connecting the stripping tube of the present invention.
  • Figure 7-1 is a split type electric egg stripping device for connecting an egg stripping tip according to the present invention.
  • Figure 7-2 is a split electric electric egg plucking device for connecting the stripping tube of the present invention.
  • Figure 8 is an exploded perspective view of the electric egg plucking device of the present invention.
  • the present invention is an electric egg stripping device, which comprises an egg peeling tube 1, a control handle 2, a power module 3, a control module 4, a display module 5, and a power module 6.
  • the electric egg-peeling device is held in a single hand in a pen-holding posture, and the thumb and the index finger hold the formed portion of the tail end of the control handle 2 which is extended downwardly and downwardly, and the middle finger assists the holding, and the control handle 2 is supported by the tiger's mouth.
  • the oviduct tube 1 may be an egg stripping tip 111, an egg stripping needle 121, or an egg stripping tube 131.
  • the oviduct tube 1 is a plug-and-play operation element, and is a disposable device to avoid cross-contamination between samples.
  • the control handle 2 is provided with a start and stop key 21, an accelerator key 22, a deceleration key 23, a connection port 24, a sealing tube 25, a power module compartment 26, a main compartment 27, and panel.
  • the steering handle 2 constitutes the main part of the housing of the electric ovipositor.
  • the power module bay 26 is mainly used to accommodate the power module 3, and the main bin 27 is mainly used to accommodate the control module 4, the display module 5, the power module 6, the memory 7, and the The tip 8 and/or the voice module 9 and/or the pressure sensor 10, etc., are primarily used to set buttons, buttons and/or interfaces.
  • the manipulation handle 2 can be designed differently according to the spawning tube 1 to which it is connected, and the stripping nozzle 111 and the peeling can be adapted as shown in FIG. 3-1-1 and FIG. 3-2.
  • the manipulation handle 2-11 of the egg needle 121 as shown in FIGS. 4-1-1 and 4-2, accommodates the manipulation handle 2-21 of the spawning tube 131.
  • the panel of the manipulation handle 2 is provided with a start and stop key 21.
  • the start and stop key 21 and the key module 43 have a program start and stop function key connected by a flexible wire.
  • the start and stop key 21 protrudes from the curved surface on the manipulation handle 2 through which the thumb naturally swings the thumb.
  • the lifting position of the starting and ending key 21 may be disposed on the left side of the holding portion of the control handle 2 to facilitate the use of the right hand, or may be disposed on the right side of the holding portion of the control handle 2 to It is convenient for the left hand to use, and can also be arranged directly in front of the holding grip of the control handle 2, and both the right hand and the left hand are convenient to use.
  • the starting position of the starting and ending key 21 is disposed directly in front of the holding section of the control handle 2.
  • the panel of the joystick 2 is provided with an accelerator button 22 and a deceleration button 23.
  • the accelerator key 22 and the deceleration key 23 are respectively connected to the button module 43 with a key having an acceleration and deceleration function through a flexible electric wire.
  • the protruding positions of the accelerator key 22 and the deceleration key 23 are disposed on the left side or the right side or the front side of the control handle 2.
  • the accelerating key 22 and the deceleration key 23 and the protruding position of the start and stop key 21 are disposed on the same longitudinal curved surface.
  • the accelerator key 22 and the deceleration The protruding position of the key 23 is disposed directly in front of the head end panel of the steering handle 2.
  • the shape, size, material and protruding height of the start and stop key 21, the accelerator key 22 and the deceleration key 23 satisfy the flexible operation requirements of most people's fingers. Preferably, different shapes, sizes and materials can be replaced according to personal preference. keycap.
  • the deceleration key 23 performs auxiliary control.
  • connection port 24 is connected to the body of the control handle 2 via a threaded interface.
  • the steering handle 2 forms a plug-in tight connection with the stripping needle 1 via a connection port 24.
  • the connection port 24 may be different according to the spawning tube 1 to which it is connected, and the different connection port 24 variants may be designed, as shown in FIG. 3-1 and FIG. 3-2 to accommodate the stripping tip 111.
  • the connection port 241 of the stripping needle 121 is connected to the connection port 242 of the stripping tube 131 as shown in FIGS. 4-1 and 4-2.
  • the central longitudinal axis of the connection port 241 is a central hole 2411, and the end of the port of the central hole 2411 is reduced to form a limit ring 2412.
  • the central hole 2411 accommodates a sealing tube 251 which is made of an elastic material such as rubber, latex, silica gel or the like.
  • the limiting ring 2412 is restrained in the central hole 2411, and the outer wall of the sealing tube 251 is closely adhered to the inner wall of the central hole 2411, and has strict airtightness.
  • the opening of the sealing tube 251 at one end of the limiting ring 2412 has an interface 2511 formed in a plug-in tight connection with the stripping needle 121, and the port 241 has an insertion with the needle stripping head 111.
  • the opening of the sealing tube 251 at the opposite end of the limiting ring 2412 has an interface 2512 which is connected with the hose connecting the micro cylinder 3111 or the micro cylinder 3111, and has a connecting tip.
  • the hose of 8 forms a tightly connected interface 2513 and/or has an interface 2514 that forms a tight connection with the hose that connects the pressure sensor 10.
  • the central longitudinal axis of the connection port 242 is a central hole 2421, and the end of the port of the central hole 2421 is reduced to form a limit ring 2422.
  • the central hole 2421 receives a sealing tube 252.
  • the sealing tube 252 is made of an elastic material, such as rubber, latex, silicone, etc., and is restrained in the central hole 2421 by the limiting ring 2422.
  • the sealing tube 252 The outer wall is in close contact with the inner wall of the central hole 2421 and has strict airtightness.
  • the opening of the sealing tube 252 at one end of the limiting ring 2422 has a plug-in tight connection with the tail end of the stripping tube 130 Interface 2521.
  • the opening of the sealing tube 252 on the opposite side of the limiting ring end has an interface 2522 which is connected with the hose connecting the micro cylinder 311 or the micro cylinder 311, and has a hose formed with the suction head 8.
  • the tightly coupled interface 2523, and/or has an interface 2524 that forms a tight connection with the hose that connects the pressure sensor 10.
  • the power module 3 includes a micro-cylinder module 31, a chute 32, the transmission mechanism 33, and the stepping motor 34.
  • the microcylinder module 31 includes a microcylinder 311, a micropush 312, and/or a sealing tube 313, and/or a pressing member 314 and/or Or press the nut 315.
  • the micro-cylinder cylinder 311 may be a micro-cylinder cylinder 311-1 of a simple circular tubular structure, or may be a micro-cylinder cylinder 311-2 that is improved on the basis of the micro-cylinder cylinder 311-1. As shown in FIG.
  • the outer wall of the micro-push rod 312 and the micro-cylinder cylinder 311-1 may constitute the micro-cylinder module 31-1 having a simple structure, and the outer wall and the outer wall of the micro-push rod 312
  • the inner wall of the microcylinder cylinder 311-1 has a small frictional force while having strict airtightness.
  • the micro-cylinder 311-1 of the micro-push rod 312 end of the central hole has an inner diameter to form a circular cavity, the corresponding outer wall has a thread to form the micro-cylinder 311-2;
  • the sealing tube 313 is made of an elastic material, such as rubber, latex, silica gel, etc., and has an inner diameter of the central hole which is the same as the outer diameter of the micro-push rod 312, and an outer diameter of which is the same as the inner diameter of the circular cavity of the micro-cylinder barrel 311;
  • the pressing member 314 is a hollow metal cylinder comprising a thick section and a thin section, the central hole inner diameter being larger than the outer diameter of the micro push rod 312, and the outer diameter of the thick section and the micro cylinder 311-2 circular.
  • the inner diameter of the cavity is the same, larger than the inner diameter of the circular opening of the opposite end of the threaded interface of the compression nut 315, and the outer diameter of the thin section is equal to the inner diameter of the circular opening of the opposite end of the threaded interface of the compression nut 315;
  • the compression nut 315 is a hollow, shaped nut having a threaded opening at one end and a circular opening at the opposite end.
  • the sealing tube 313 is received in a circular cavity of the micro-cylinder barrel 311-2, and the pressing piece 314 is closely attached to the sealing tube 313, and the pressing nut 315 and the micro-cylinder barrel 311 After the threaded connection of -2, the thick section and the partial section of the pressing member 314 are located in the pressing nut 315, and the remaining portion of the thin section protrudes from the circular opening of the pressing nut 315. As shown in FIG. 5-2, the micro-push rod 312 is inserted into the micro-cylinder barrel 311-2 through the central hole of the pressing member 314 and the central hole of the sealing tube 313 to form the micro-cylinder tube module. 31-2.
  • the pressing nut 315 presses the sealing tube 313 to deform by the pressing member 314, and can be adjusted by adjusting the thread height of the pressing nut 315 and the micro cylinder 311-2 to be screwed.
  • the degree of radial deformation of the sealing tube 313 The airtightness and frictional force of the sealing tube 313 and the micro-push rod 312 are adjusted.
  • the transmission mechanism 33 in the power module 3 performs the following two settings according to the principle: a screw mechanism 3311, a nut-slider 3312 as a transmission mechanism of a transmission component.
  • the 331 is a transmission mechanism 332 having a spur gear 3321 and a straight rack-slider 3322 as main transmission members. One of them is selected as the transmission mechanism of the electric spawning device.
  • the nut and the slider are integrally designed, and the screw rod 3311 is an output section of the rotating shaft of the stepping motor 34; the sliding slot 321 can be fixed.
  • the stepping motor 34 can fix the microcylinder module 31; one end of the micro push rod 312 is fixed on the nut-slider 3312.
  • the stepping motor 34, the chute 321, the nut-slider 3312 and the microcylinder module 31 constitute the power module 3-1, which is convenient for assembly and saves space.
  • the straight rack and the slider are integrally designed, and the spur gear 3321 is assembled in an output section of the rotating shaft of the stepping motor 34; the sliding slot 322
  • the stepping motor 34 may be fixed or the microcylinder module 31 may be fixed; one end of the micro push rod 312 is fixed to the straight rack-slider 3322.
  • the stepping motor 34, the chute 322, the straight rack-slider 3322 and the microcylinder module 31 constitute the power module 3-2, which is convenient for assembly and saves space.
  • the function of the transmission mechanism 33 is to convert the forward rotation and the reverse rotation of the stepping motor 34 into a linear reciprocating motion of the micro-push rod 312.
  • the step angle of the stepping motor 34 and the pitch of the lead screw 3411 determine a linear stroke of the single pulse of the stepping motor 34; in the power module 3-2
  • the step angle of the stepping motor 34 and the pitch circle diameter of the spur gear 3421 determine a linear stroke of the single pulse of the stepping motor 34.
  • the physical parameters of the transmission mechanism 33 such as the screw pitch, the lead, the spur gear index circle diameter and the stepping motor 34 step angle can meet the requirements of the spawning procedure to adjust the liquid column length.
  • the stepping motor 34 in the power module 3 is preferably a 2-phase four-wire stepping motor. In order to make the electric spawning device more lightweight, it is preferably smaller in size and capable of satisfying the process of performing the spawning procedure. The stepper motor 34 is less expensive.
  • the power module 3 is disposed in the power module compartment 26 of the control handle 2, and the power module 3 can also be disposed in the control box 11 in the split type electric ovipositor.
  • the control module 4 includes a stepping motor driver 41, a single chip microcomputer 42, and a button module 43.
  • the stepping motor driver 41, the single chip microcomputer 42 and the button module 43 may adopt an integrated circuit module.
  • the button module 43 includes at least a key or an electrical connection point having a program selection, start and stop, acceleration, deceleration, increase stroke, and a decrease stroke function.
  • different functions can be combined and implemented on one key. For example, two presses of the same key phase can perform different functions, and the same button press duration can be different to perform different functions, and the same number of consecutive presses can be executed differently. Different functions, etc.
  • the keys of the button module 43 may be button buttons or touch buttons or the like.
  • the display module 5 includes a display screen 51 and a button module 52.
  • the display screen 51 is used to display basic parameters of the running program, such as program number, blowing frequency, liquid column length, and/or operating data, such as real-time blowing frequency, starting and ending time of a certain blowing frequency, and accumulation of a certain blowing frequency. Information such as operating time, total operating time, and/or battery or battery power, and/or alarms.
  • the display screen 51 is an LED or LCD display screen or a touch display screen, and supports multi-language display. The language includes at least Chinese, English and the like. Can be black and white or color display.
  • the display screen 51 is mounted on the side of the front end panel of the steering handle 2 and/or directly in front of the control box 11 on the side of the head end panel of the control handle 2 or on the side of the thumb of the gripped hand.
  • the display screen 51 of the appropriate size and resolution is selected and the appropriate display content is selected. If the display screen 51 is only mounted on the head end panel of the control handle 2, preferably has a high resolution display screen, the size of the display screen 51 conforms to the electric spawning device while satisfying the basic parameters of the display operation program. The overall light and beautiful requirements.
  • the display screen 51 on the control handle 2 only needs a display screen with a smaller size and a relatively lower resolution.
  • the display screen 51 on the control box 11 requires a larger size and higher resolution display screen, which satisfies the need for the operator to clearly recognize the display content in a dark field of view within a viewing distance of 1 meter.
  • the display screen 51 having a lower energy consumption is preferable on the premise that the above conditions can be satisfied.
  • the keys of the button module 52 may be button buttons or touch buttons or the like.
  • the function of the button module 52 is adapted to the display 51.
  • some of the keys and the keys of the button module 43 of the control module 4 are combined, so that the number of keys is as small as possible, and the operation interface is more simple. .
  • the power module 6 includes a charging module 61 and an electrical energy storage unit 62.
  • the charging module 61 charges the electrical energy storage unit.
  • the electrical energy storage unit is a battery or a battery similar to a lithium ion battery.
  • the charging module 61 and the electrical energy storage unit 62 which are smaller in volume and lighter in weight, are preferably provided on the premise that the electric power demand of the electric ovipositor can be met.
  • the power module 6 is disposed in the main compartment 27 of the control handle 2 or in the control box 11.
  • the memory 7 is configured to store program running data, including operation object information, such as a number, and the corresponding egg stripping operation data of the operation object, such as a program number, a blowing frequency, a liquid column length, and program running data, such as real-time. Data such as the suction frequency, the start and end time of a certain suction frequency, the accumulated operation time of a certain suction frequency, and the total operation time.
  • the memory 7 is electrically connected to the control module 4, and is disposed in the housing of the control handle 2 or in the control box 11.
  • the tip 8 has a cylindrical shape or a spherical shape and is an elastic material such as rubber, latex, silica gel or the like.
  • the suction head 8 is disposed on the grip portion of the manipulation handle 2, the connection end is buried under the panel of the manipulation handle 2, the blind end is exposed to the outside, and the control handle is at an angle, such as 30 degrees. 2 The end is inclined.
  • the connecting end of the tip 8 is in communication with the spawning tube 1 through a pipe.
  • the specific position of the tip 8 is arranged in cooperation with other operating elements on the control handle 2, such as the start and stop key 21, the accelerator key 22 and the deceleration key 23.
  • the suction head 8 is adjacent to the start and stop key 21 and longitudinally distributed along the manipulation handle 2, and the exposed portion of the suction head 8 is located at the outer end of the thumb of the hand holding the electric ovipositor.
  • the thumb can perform a shifting operation on the start and stop key 21 and the suction head 8 only by naturally swinging left and right.
  • the maximum volume variable formed by the thumb pressing the tip 8 in a normal operating position is the effective emptying volume of the tip 8, and the effective emptying volume is smaller than the volume of the stripping needle 12 or the stripping tip 111. .
  • the electric ovipositor can also be provided with a speech module 9.
  • the operator's eyes or single eyes are close to the microscope eyepiece to observe the process of spawning.
  • some information on the display screen may be real-time.
  • the blowing frequency and the like are encoded by sound, and are played through the speaker, and the operator obtains the information through the sound; the stripping operation belongs to the zygote embryo in vitro operation, and has strict in vitro operation time limit. Through the microcontroller programming, the total operating time and / or timeout reminder can be notified to the operator by voice.
  • the voice module 9 is disposed in the main compartment 27 of the control handle 2 or in the control box 11.
  • the electric spawning device can also be provided with a pressure sensor 10.
  • the pressure sensor 10 monitors the pressure change of the communication pipe between the micro push rod 312 and the spawning tube 1, and submits the pressure information to the single chip microcomputer system, and the single chip system automatically adjusts and controls the stepping motor according to the pressure change value. Pulse frequency, the purpose of automatic speed control. When the egg-crown complex is passed through the tip of the egg-peeling needle 1 and passes slowly, the conventional method of spawning is accelerated by increasing suction or blowing force. The judgment and processing method made by each operator on this situation is greatly influenced by subjective factors, which is not conducive to standardization.
  • the low pressure value can be measured when the egg-crown complex is caught by the tip of the egg-peeling needle 1 and the pressure is lowered when the passage is slow.
  • the pressure sensor 10 detects the lower limit of the threshold, it is fed back to the single-chip microcomputer 42 to automatically increase the pulse frequency of the stepping motor 34 to a set high value according to a program setting, thereby increasing the blowing frequency and increasing the suction force. And blowing force, so that the egg-crown complex smoothly passes through the mouth of the oviduct. After the set number of blowing times is set to a high value, the original blowing frequency is restored, thereby achieving the purpose of automatically adjusting the blowing force and the suction force.
  • the automatic adjustment of the blowing frequency is triggered by the pressure of the connecting pipe between the micro-pushing rod 312 and the spawning tube 1 being lower than the lower threshold, and is not subjective to the operator. Factor influence, easy to standardize.
  • the pressure sensor 10 is disposed within a housing of the steering handle 2 .
  • An automatic speed control button for activating/deactivating the function is provided on the panel of the manipulation handle 2.
  • the control box 11 is used as a carrier for mounting other components. When the components are physically separated from the control handle 2, separate components are installed in the control box 11, and corresponding buttons and buttons are disposed on the panel. interface.
  • the connection relationship between the main components of the electric ovipositor is as follows: the stepping motor driver 41, the button module 43, the display module 5, the power module 6, and the memory 7
  • the voice module 9 and the pressure sensor 10 are respectively connected to the single chip microcomputer 42.
  • the output end of the stepping motor driver 41 is connected to the stepping motor 34.
  • the foot switch controller 12 is connected to the button.
  • the corresponding button of module 43 is electrically connected.
  • the output shaft of the stepping motor 34 is connected to the input end of the transmission mechanism 33.
  • the output end of the transmission mechanism 33 is connected to one end of the micro-push rod 312, and the other end of the micro-push rod 312 is disposed.
  • the micro-cylinder cylinder 311 communicates with the spawning pipe 1 through a pipe or directly through a sealing pipe 251 or through a sealing pipe 252, and the suction pipe 8 passes through the pipe and the peeling pipe
  • the egg tubes 1 are in communication, and the pressure detecting element of the pressure sensor 10 is in communication with the spawning tube 1 through a pipe.
  • the steering handle 2 forms a plug-in tight connection with the spawning tube 1 via the connection port 24.
  • the main components of the electric egg plucking device are fixedly assembled in the steering handle 2 to be assembled into an integrated electric ovulator.
  • the remaining components are physically separated from the control handle 2 and fixedly assembled in the control box 11 to form a split electric unit.
  • Ovum peeler In the split electric omelette, the relevant components are connected by a flexible wire or a flexible pipe.
  • the spatial layout of the main components of the electric ovipositor can be designed in the following five ways. Each design method constitutes an electric ovipositor with similar functions.
  • Manner 1 The spawning tube 1 and the control handle 2 are connected to each other to form a plug-in type, the power module 3, the control module 4, the display module 5, the power module 6, the memory 7.
  • the suction head 8, the voice module 9, and the pressure sensor 10 are mounted in the control handle 2 to form an integrated electric spawning device.
  • Manner 2 the spawning tube 1 and the control handle 2 connection port form a plug-in close connection, the power module 3, the control module 4, the display module 5, the memory 7, the nozzle 8.
  • the voice module 9 and the pressure sensor 10 are mounted in the control handle 2, and the power module 6 is installed in the control box 11.
  • the control box 11 can also mount one of the display modules, and the display module is connected to the control module 4 by a flexible wire.
  • the control box 11 is physically separated from the control handle 2 to form a split electric scraper. It is also possible to provide a foot switch controller 12 physically separate from the above-described elements, in which case the start and stop keys 21, the accelerator keys 22 and the deceleration key 23 on the panel of the control handle 2 can be retained. Or cancel.
  • Manner 3 the spawning tube 1 and the control handle 2 are connected to each other to form a plug-in tight connection, and the power module 3, the display module 5, the suction head 8, and the pressure sensor 10 are installed in the Within the control handle 2, the control module 4, the power module 6, the memory 7 and the voice module 9 are mounted in the control box 11.
  • the display module 5 in the manipulation handle 2 passes A flexible wire is connected to the control module 4.
  • Some of the keys of the button module of the control module 4, such as keys for controlling the start and stop, acceleration and deceleration functions, are connected to the start and stop keys, the accelerator keys and the deceleration keys of the control handle 2 via flexible wires.
  • the pressure detecting element of the pressure sensor 10 is connected to the control module 4 by a flexible wire.
  • the control box 11 is physically separated from the control handle 2 to form a split electric scraper. It is also possible to provide a foot switch controller 12 physically separate from the above-described elements, in which case the start and stop keys 21, the accelerator keys 22 and the deceleration key 23 on the panel of the control handle 2 can be retained. Or cancel.
  • Manner 4 the peeling tube 1 and the connecting handle of the control handle 2 form a plug-in tight connection, and the display module 5, the suction head 8, and the pressure sensor 10 are installed in the control handle 2,
  • the power module 3, the control module 4, the power module 6, the memory 7 and the voice module 9 are mounted in the control box 11.
  • the display module 5 in the control handle 2 is connected to the control module 4 by a flexible wire.
  • One of the display modules may also be mounted in the control box 11, and the display module is connected to the control module 4 by a flexible wire.
  • Some of the keys of the button module of the control module 4, such as keys for controlling the start and stop, acceleration and deceleration functions, are connected to the start and stop keys, the accelerator keys and the deceleration keys of the control handle 2 via flexible wires.
  • the micro-cylinder cylinder 311 in the power module 3 communicates with the spawning tube 1 through a flexible pipe, and a connecting pipe between the micro-push rod 312 and the spawning pipe 1 is filled with a liquid, such as minerals. oil.
  • the pressure detecting element of the pressure sensor 10 is connected to the control module 4 by a flexible wire.
  • the control box 11 is physically separated from the control handle 2 to form a split electric scraper. It is also possible to provide a foot switch controller 12 physically separate from the above-described elements, in which case the start and stop keys 21, the accelerator keys 22 and the deceleration key 23 on the panel of the control handle 2 can be retained. Or cancel.
  • the fifth embodiment the peeling tube 1 and the connecting handle of the control handle 2 form a detachable tight connection, the suction head 8 and the pressure sensor 10 are installed in the control handle 2, and the power module 3
  • the control module 4 the display module 5, the power module 6, the memory 7 and the voice module 9 are installed in the control box 11.
  • the micro-cylinder cylinder 311 in the power module 3 communicates with the spawning tube 1 through a flexible pipe, and a connecting pipe between the micro-push rod 312 and the spawning pipe 1 is filled with a liquid, such as minerals. oil.
  • the pressure detecting element of the pressure sensor 10 passes A flexible wire is connected to the control module 4.
  • the control box 11 is physically separated from the control handle 2 to form a split electric scraper. It is also possible to provide a foot switch controller 12 physically separate from the above-described elements, in which case the start and stop keys 21, the accelerator keys 22 and the deceleration key 23 on the panel of the control handle 2 can be retained. Or cancel.
  • the method of spawning using the electric egg-peeling device of the present invention is as follows: before the ICSI or before the freezing of the eggs, the egg-nosed complex is transferred into the hyaluronidase solution containing 80 IU/L by using the undrawn bentonian tube. Continuously blowing several times to remove most of the mucus mass, and then transferring the pre-digested egg-crown complex into the operating medium, and the stepping motor 34 provides the suction power to repeatedly advance and exfoliate the pre-digested egg-crown complex. The tip of the egg tube until the level of granulosa cells is removed to meet operational requirements;
  • the method of peeling the eggs by using the electric egg-peeling device of the present invention can also be as follows: the eggs are peeled after IVF in a test tube method, and the IVF complex medium suspension after IVF in the test tube method is transferred to an empty space by using an undrawn Babbitt tube.
  • the culture dish is provided by the stepping motor to provide the suction and suction power to repeatedly enter and exit the mouth of the spawning tube until the granular cells are removed to meet the operational requirements;
  • the microdropping method is used to strip the eggs after IVF, and the stepping motor 34 provides the suction.
  • the power is repeatedly sucked and sucked with the oocytes of the granule cells attached to the bottom of the culture dish to be detached from the bottom of the dish, and then repeatedly sucked until the granule cells are removed to meet the operational requirements.
  • the stepper motor 34 is controlled by a single chip microcomputer 42.
  • the single chip microcomputer 42 has a single chip program, and the parameters of the program mainly include the blowing frequency and the liquid column length.
  • the length of the suction column is adjusted by the stroke of the stepper motor 34.
  • the stepping motor 34 adjusts the blowing frequency by the round-trip frequency, and accordingly adjusts the blowing speed and the corresponding blowing power.
  • the faster the blowing frequency, the faster the speed, and the blowing power is also The bigger.
  • the MCU program can be modified and/or stored as a new program at any time, and can be started and stopped at any time.
  • the stepping motor 34 includes at least three speed regulation modes: alternately using the pre-stored program speed regulation, manually increasing or decreasing the single amplitude on the basis of the operating program blowing frequency, the programmed frequency value can be programmed, On the basis of the blowing frequency, it automatically accelerates to a certain value of the blowing frequency and continues to the original blowing frequency after the set time.
  • the egg stripping operation data is recorded in the memory and can be viewed and/or output.
  • the data of the oviducting operation mainly includes: basic parameters of the running program, such as the program number, the suction frequency, the liquid column length and the operation data, such as the real-time blowing frequency, the starting and ending time of a certain suction frequency, the cumulative operation time of a certain blowing frequency, and the total Data such as operating time.

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Abstract

一种电动剥卵装置及其剥卵方法。该电动剥卵装置包括剥卵管（1）、操控手柄（2）、动力模块（3）、控制模块（4）、显示模块（5）、电源模块（6）、内存（7）、吸头（8）和/或语音模块（9）、和/或压力传感器（10）、和/或控制盒（11）、和/或脚踏开关控制器（12），可以组装成一体式或分体式电动剥卵装置。该电动剥卵装置由步进电机（34）提供吹吸动力剥除卵母细胞外周的颗粒细胞。该电动剥卵装置及其剥卵方法，精确性好，能避免剥卵相关风险，实现剥卵操作标准化，降低劳动强度。

Description

一种电动剥卵装置及其剥卵方法
本申请要求于2014年07月06日提交中国专利局、申请号为201410317452.2、发明名称为“一种电动剥卵装置及其剥卵方法”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本发明涉及一种生殖实验室设备，具体涉及一种电动剥卵装置及其剥卵方法，属于实验室显微操作技术领域。
背景技术
剥卵是生殖实验室日常工作重要内容，包括：剥除经酶预消化的卵冠丘复合体的颗粒细胞，以便评估卵母细胞成熟度和便于显微操作，如单精子胞浆内注射((Intracytoplasmic sperm injection，简称ICSI)、卵母细胞冷冻前需要剥除颗粒细胞；体外授精(in vitro fertilization，简称IVF)，如微滴法IVF、试管法IVF后需要剥除卵母细胞外周的颗粒细胞，以便评估受精情况。剥卵在上述操作中占据重要地位，直接影响后续操作环节的效果。
数十年来，剥卵工具基本保持不变，完全通过手动方法操作。传统剥卵管包括剥卵针和剥卵巴氏管，分别套上相应的乳胶吸头组成剥卵针剥卵器和巴氏管剥卵器。传统剥卵方法是主要是通过手指反复按压乳胶吸头提供吸吹力量使经透明质酸酶预消化的卵冠丘复合体或行试管法IVF后被精子消化后的卵冠丘复合体进出剥卵管管口剥除颗粒细胞，或者使行微滴IVF后粘附在培养皿底的卵冠丘复合体中的卵母细胞在吸力的作用下脱离复合体，再经过反复吹吸剥去其余颗粒细胞。也有将剥卵管用软管连接口吸管组成口吸管式剥卵器，将吸嘴放入口腔中，通过口腔吹吸使冠丘复合体进出剥卵管管口剥除颗粒细胞。根据每个卵冠丘复合体颗粒细胞结合的紧密程度，剥去的难易程度不同，吸吹次数由数次至数十次不等。
专利“口腔球囊式剥卵器”(专利号：CN200720157432.9)是一种对卵母细胞周围的卵冠丘复合物进行剥离的口腔球囊式剥卵器。它包括微吸管，在 微吸管的后端套接有软管，在软管的末端连接有软球囊。操作时，将球囊放入口腔上下牙齿之间，通过咬合牙齿来控制球囊提供吹吸力量，使卵母细胞反复地轻柔地进出微吸管尖端，达到剥除卵母细胞周围大部分颗粒细胞的目的。该发明将口吸管的吸嘴换成球囊，通过牙齿咬合挤压球囊达到吸吹的效果。该方法和口吸管方式相比，可以避免口腔呼气污染卵母细胞的风险。
专利“剥卵针”(专利号：CN201220219074.0)提供一种适合所有大小卵母细胞的剥卵针。该发明用于剥离卵母细胞周围颗粒细胞的围颗粒细胞的中空剥卵针。该发明只针对剥卵针的结构进行了改变，剥卵方式和传统方法相同。
专利“一种新型试管婴儿剥卵操作器”(专利号：CN201320050412.7)提供的剥卵操作器由手持按压装置和吸取卵母细胞装置构成，手持按压装置由压杆帽、笔帽、后壳、弹簧、压杆和前壳构成，吸取卵母细胞装置由气囊、PS管、针座和剥卵管构成，气囊、PS管、针座和剥卵管从上至下依次装在后壳的中下部；前壳的中部装有与滑扣连接的推钮。该发明在操纵性上比传统的乳胶吸头有了明显提高。
然而，现有技术的剥卵设备存在以下不足。
第一，控制不精确，存在多种风险。现有剥卵技术中，由操作者的肌肉力量控制剥卵器弹性操作元件的乳胶吸头、口吸管、口腔球囊、气囊。乳胶吸头、口腔球囊和气囊在受到外力作用发生压缩变化时，其材质所拥有的弹性具有解除压缩形变恢复原状的能力，是此类手动剥卵器的产生吸力的动力来源。操作者施加于上述弹性操作元件原件的挤压力是此类手动剥卵器产生吹力的动力来源。在口吸管剥卵器中，口腔的作用类似于上述剥卵器的弹性操作元件，不同点在于，吹和吸都是操作者主动施力的过程。在吸力和吹力发生和转换过程始终伴随按压力与弹性元件复原力之间的对抗性平衡。在剥卵时，尤其是剥卵开始阶段，卵冠丘复合体比剥卵管管口内径稍大，通过剥卵管管口时会发生堵的现象，表现为卵冠丘复合体通过剥卵管管口速度减慢。操作者在调整增加吸力或者吹力，尤其是增加吹力后，一旦卵冠丘复合体通过剥卵管管口，操作者无法及时制动，相接的吸和吹两个过程失衡，发生的体积变化不一致，吹产生的体积变化大于吸产生的体积变化，导致吹出一连 串气泡。卵冠丘复合体或卵母细胞被气泡吸附于其边缘，随气泡漂浮在培养基液滴表面。剥卵操作界面与气泡存在于不同的焦距面，被气泡吸附的卵冠丘复合体或卵母细胞也可以位于气泡表面的任何位置，在气泡边缘寻找卵冠丘复合体或卵母细胞需要不断的调整显微镜焦距，致使整个剥卵操作时间延长，不仅增加因体外操作时间延长损伤卵母细胞的风险，还存在导致丢失卵母细胞的重大风险。操作者为了尽可能的避免气泡产生，一般事先用剥卵管吸取几微升培养基滞留在剥卵管尖端，用于吹吸体积不一致时的补偿，使得剥卵管内液柱长度远远超出培养皿内液体高度。
现有剥卵方法控制不精确使卵母细胞或合子处于置于温度波动引发的风险。温度是影响卵母细胞、合及胚胎质量的最重要因素之一。人的基础体温是37℃，在体外操作是尽量保持卵母细胞或合子所在环境的温度为37℃。一般的，为了有更清洁的空气，剥卵操作在通风的超净台内进行，超净台面的温度约37℃，室温约25℃，卵冠丘复合体所在的培养皿内的培养基温度与超净台面温度接近。剥卵管尖部非常细小，手动剥卵时，剥卵管尖部内的培养基液面远远高于培养皿液面，没入培养皿内培养基的尖端仅具有约0.1μL的培养基，其温度接近培养皿内的培养基，其余部分暴露在培养皿内培养基之外。这样剥卵管存在温度截然不同的两个区域：第一区域，没入培养皿培养基液面下的尖端，温度和培养皿培养基接近；第二区域，未没入培养皿培养基之内的剩余部分，温度和室温接近。当被吸入剥卵管内的液柱长度超出培养皿液面高度后，热量通过剥卵管玻璃管壁被风迅速带走，卵母细胞或合子在温差超几度的环境来回移动，存在因温度波动导致损伤卵母细胞或合子的风险。低温对卵母细胞和合子的影响包括降低其代谢水平和改变细胞器形态及功能。比如，在33℃的温度下，纺锤体会在5分钟内开始解聚，10分钟内完全消失。如果将同一卵子加热到37℃，纺锤体可以在10分钟内完全恢复。如果温度降到28℃，纺锤体解聚速度加快，到完全消失的时间缩短，同时加温后恢复的时间会更长。如果在25℃的环境下，纺锤体解体后加温至37℃后20分钟内很难恢复。在恢复期内，如果存在细胞周期变化将会导致染色体数目异常。一般认为，人卵母细胞及合子在体外操作过程中的温度一定要保持在一个比较恒定的范围之内，如35～37℃，在操作过程中尽量减少温度的波 动。剥卵时，为了尽量减少这种风险，需要吸入剥卵管内的液柱位于培养皿液滴之下。以卵冠丘复合体所在培养皿培养基的高度约2.5mm，剥卵管与培养皿底夹角30度为例，剥卵管中液柱长度在5mm以下，才可以保证剥卵管中培养基液面位于培养皿液滴液面之下。剥卵管尖端非常细小，管尖内径一般在150μm，管尖中高度为5mm培养基的体积约为0.1μL。在体积如此微小的培养基里，通过操作者肌肉力量控制剥卵器的弹性操作元件使卵冠丘复合体来回进出剥卵管，不产生气泡又能够适当地调整速度几乎无法实现。
第二，操作主观性强，无法标准化。剥卵操作要求轻柔吹打，但无法对“轻柔吹打”进行明确定义，不同操作者对“轻柔吹打”主观感受不同，表现为在使用上述传统剥卵器时按压乳胶吸头、弹簧压杆、呼气/吸气、牙齿咬合的深浅及快慢存在巨大差异。这些差异直接表现为吸入剥卵管液柱长度差异和卵冠丘复合体通过剥卵管口速度的差异，间接表现为温度波动所导致的损伤卵母细胞风险的高低和对卵冠丘复合体作用力大小的差别。液体的流速与压强呈正变关系，当喷口直径固定时，液体流速越快，喷口压强越大。通过控制吹吸频率可以调整液体流速，达到调整喷口压强，进而调整作用卵冠丘复合体吸力和吹力。亦即吹吸频率增加，作用卵冠丘复合体吸力和吹力增大，进一步的，剥离颗粒细胞的作用力也相应的增大，剥离速度加快。因此，通过调整吹吸频率可以调整剥卵速度。现有剥卵器通过按压乳胶吸头、弹簧压杆、呼气/吸气、牙齿咬合的深浅及快慢调整作用于卵冠丘复合体吸力和吹力。完成一个卵冠丘复合体的剥卵过程一般的会采用几种吹吸速度，相应的存在几种吹力和吸力。不同的吹力和吸力及其持续时间，尤其是过于剧烈的吹力和吸力及其持续时间，对卵母细胞的潜在负面影响程度不同，吹吸过于剧烈会导致卵母细胞破裂瓦解。手动剥卵时，操作者完全依照主观经验做出判断调整，对具体操作细节无法进行量化，相应的无法对整个剥卵过程提供客观具体的数据。无论是胚胎质差还是质优，试图在剥卵环节分析原因时，都无法通过回顾剥卵操作过程或者比较不同操作者的操作得出有价值的结论。
第三，工作强度大。现有剥卵方法每一次吹吸卵冠丘复合体都必需对应一次按压/松开弹性元件或口腔吹/吸的动作。现有剥卵器无论是采用单手还 是双手操作，或者口腔控制，操作者为了获得最佳的控制效果，维持在吸力和吹力转换过程始终伴随的对抗性平衡，手指或者口腔肌肉一直处于紧张状态，造成操作者体力上的疲劳。又因为传统剥卵器存在时间延长损伤卵母细胞的风险、温度波动导致损伤卵母细胞的风险以及丢失卵母细胞的重大风险，造成操作者心理负担增加。
因此，为解决上述技术问题，确有必要提供一种先进的电动剥卵装置及其剥卵方法，以克服现有技术中的所述缺陷。
发明内容
为解决上述问题，本发明的目的在于提供一种精确性好、标准化高、舒适性强且自动化程度高的电动剥卵装置。
本发明的另一目的在于提供一种采用上述电动剥卵装置的剥卵方法。
为实现上述第一目的，本发明采取的技术方案为：一种电动剥卵装置，其包括剥卵管，操控手柄，安装于操控手柄内的动力模块、控制模块、电源模块和内存以及吸头；其中，所述操控手柄的一端设有连接端口，所述剥卵管连接于连接端口上；所述动力模块包括微缸筒模块、传动机构以及步进电机；所述控制模块包括步进电机驱动器、单片机以及按键模块；所述步进电机驱动器、按键模块、显示模块、电源模块、内存分别与所述单片机连接；所述步进电机驱动器与所述步进电机连接；所述步进电机与所述传动机构连接，所述传动机构与一微型推杆连接；所述微型推杆的另一端置于所述微型缸筒内，所述微型缸筒与所述剥卵管密封管连通；所述吸头与所述剥卵管相连通。
本发明的电动剥卵装置进一步设置为：所述剥卵管具体为剥卵吸头、剥卵针或剥卵巴氏管。
本发明的电动剥卵装置进一步设置为：于所述操控手柄上设置有起止键、加速键和减速键，该起止键、加速键和减速键分别和按键模块电性连接。
本发明的电动剥卵装置进一步设置为：于所述操控手柄内设有动力模块仓和主仓；所述动力模块收容于动力模块仓内；所述控制模块、电源模块收容于主仓内；于所述操控手柄上还嵌设有一显示屏。
本发明的电动剥卵装置进一步设置为：所述连接端口的中央设有一中央孔，所述中央孔的端口末端缩小形成一个限位环；所述中央孔容纳一个密封管，所述限位环限位在所述中央孔内，所述密封管外壁与所述中央孔内壁紧密贴合；所述密封管的一端的开口具有与所述剥卵管形成插入式紧密连接的接口；所述密封管的另一端开口具有与连通微型缸筒形成紧密连接的接口，与连通吸头形成紧密连接的接口，和与连接压力感应器形成紧密连接的接口。
本发明的电动剥卵装置进一步设置为：所述微缸筒模块包括微型缸筒、密封管、压紧件以及压紧螺帽；其中，所述微型推杆和微型缸筒连接；所述密封管容纳于所述微型缸筒内，所述压紧件紧贴所述密封管，所述压紧螺帽与所述微型缸筒的螺纹连接，其一部分位于所述压紧螺帽内，另一部分伸出所述压紧螺帽的圆形开口。
本发明的电动剥卵装置进一步设置为：所述传动机构包括丝杆和螺母－滑块；其中，所述丝杆连接所述步进电机；所述微型推杆的一端固定在所述螺母－滑块上。
本发明的电动剥卵装置进一步设置为：所述传动机构包括直齿轮和直齿条－滑块；其中，所述直齿轮组装在所述步进电机上；所述微型推杆的一端固定在所述直齿条－滑块上。
本发明的电动剥卵装置进一步设置为：其还设有一语音模块，所述语音模块与所述单片机连接。
本发明的电动剥卵装置进一步设置为：其进一步可包括一控制盒，所述控制盒通过线缆连接至操控手柄上。
本发明的电动剥卵装置进一步设置为：于所述控制盒上设有一显示屏；或者在所述操控手柄和控制盒上分别设有一显示屏。
本发明的电动剥卵装置还设置为：其还可包括一压力传感器，该压力传感器亦与单片机连接，该压力传感器的压力检测元件与所述剥卵管通过管道连通。
为实现上述第二目的，本发明采取的技术方案为：一种剥卵方法，其包括如下步骤：用未拉制的巴氏管将卵冠丘复合体转移入透明质酸酶溶液内，连续吹打数次，去除大部分粘液块，然后将预消化的卵冠丘复合体转移到操 作培养基内，由步进电机提供吹吸动力使预消化的卵冠丘复合体反复进出剥卵管嘴部，直到去除颗粒细胞程度达到操作要求。
本发明的剥卵方法进一步为：所述步进电机由单片机控制，其至少包括三种调速方式：交替使用预存程序调速、在运行程序吹吸频率的基础上手动增加或降低单次幅度可以通过编程设定的频率值、在运行程序吹吸频率的基础上自动加速至某一设定吹吸频率高值并持续至设定时间后恢复原有吹吸频率。
为实现上述第二目的，本发明采取的另一技术方案为：一种剥卵方法，其包括如下步骤：用未拉制的巴氏管将试管法IVF后的卵冠丘复合体培养基悬液转移至空的培养皿，由步进电机提供吹吸动力使卵冠丘复合体反复进出剥卵管嘴部，直到去除颗粒细胞程度达到操作要求；微滴法IVF后剥卵，由步进电机提供吹吸动力反复吹吸随颗粒细胞附壁在培养皿底的卵母细胞，使之从皿底脱落，再经反复吹吸直到去除颗粒细胞程度达到操作要求。
本发明的剥卵方法还可为：所述步进电机由单片机控制，其至少包括三种调速方式：交替使用预存程序调速、在运行程序吹吸频率的基础上手动增加或降低单次幅度可以通过编程设定的频率值、在运行程序吹吸频率的基础上自动加速至某一设定吹吸频率高值并持续至设定时间后恢复原有吹吸频率。
与现有技术相比，本发明具有如下有益效果：
1.定位精准，安全有效，剥卵操作不产生气泡不仅缩短操作时间还避免丢失卵母细胞，控制精确，避免卵母细胞所在培养基温度大幅度波动，可以最大限度减少温度波动对卵母细胞的损伤，对改善妊娠结局具有积极意义。
2.剥卵操作过程具有量化指标，减少主观因素影响，剥卵操作记录可以保存并查询，解决了传统剥卵技术不同操作者间剥卵操作无法比较、同一操作者既往操作无法回顾分析的问题，对实现剥卵操作标准化具有重要意义。
3.自动化程度高，只需起止键即可按照设定程序剥卵，通过加速键、减速键方便实现手动加速、减速，还可以通过自动调速实现自动加速而后恢复原速，控制简单，操作轻松，相比传统剥卵方法，对改善劳动强度具有显著意义。
附图说明
图1为本发明的电动剥卵装置原理示意图；
图2－1为剥卵吸头的示意图；
图2－2为剥卵针的示意图；
图2－3为剥卵巴氏管的示意图；
图3－1－1为本发明一体式电动剥卵器操控手柄的爆炸视图；
图3－1－2为图3－1－1的连接端口和密封管的局部视图；
图3－2为本发明分体式电动剥卵器操控手柄和控制盒的立体视图；
图4－1－1为本发明一体式电动剥卵器操控手柄的爆炸视图；
图4－1－2为图4－1－1的连接端口和密封管的局部视图；
图4－2为本发明分体式电动剥卵装置操控手柄和控制盒的立体视图；
图5－1－1为本发明的电动剥卵装置的微缸筒模块及微缸筒模块的爆炸视图；
图5－1－2为本发明的电动剥卵装置的微缸筒模块及微缸筒模块另一形式的爆炸视图；
图5－2为本发明的电动剥卵装置的动力模块和动力模块立体视图；
图5－3为本发明的电动剥卵装置的动力模块和动力模块另一形式的立体视图；
图6－1为本发明连接剥卵吸头的一体式电动剥卵装置；
图6－2为本发明连接剥卵针的一体式电动剥卵装置；
图6－3为本发明连接剥卵巴氏管的一体式电动剥卵装置；
图7－1为本发明连接剥卵吸头的分体式电动剥卵装置；
图7－2为本发明连接剥卵巴氏管的分体式电动剥卵装置；
图8为本发明的电动剥卵装置的立体分解图。
具体实施方式
请参阅说明书附图1至附图8所示，本发明为一种电动剥卵装置，其由剥卵管1、操控手柄2、动力模块3、控制模块4、显示模块5、电源模块6、内存7、吸头8和/或语音模块9和/或压力传感器10和/或控制盒11等。
所述电动剥卵装置以执笔式姿势单手持握，拇指和食指握住所述操控手柄2尾端向下延伸缩小的形成的细部，中指辅助持握，所述操控手柄2托于虎口。
如图2－1至2－3所示，所述剥卵管1可以为剥卵吸头111、剥卵针121或剥卵巴氏管131。所述剥卵管1为即插即用的操作元件，为避免样本间交叉污染，属一次性器材。
如图3－1－1至图4－2所示，所述操控手柄2设置起止键21、加速键22、减速键23、连接端口24、密封管25、动力模块仓26、主仓27及面板。所述操控手柄2构成所述电动剥卵器的壳体的主要部分。所述动力模块仓26主要用于容纳所述动力模块3，所述主仓27主要用于容纳所述控制模块4、所述显示模块5、所述电源模块6、所述内存7、所述吸头8和/或所述语音模块9和/或所述压力传感器10等，面板主要用于设置按键、按钮和/或接口。
所述操控手柄2根据其连接的所述剥卵管1不同，可以设计不同变体，如图3－1－1和图3－2所示的适应所述剥卵吸头111和所述剥卵针121的操控手柄2－11，如图4－1－1、图4－2所示的适应所述剥卵巴氏管131的操控手柄2－21。
所述操控手柄2的面板设置一个起止键21。所述起止键21与按键模块43具有程序起止功能的键通过柔性电线连接。
所述起止键21凸出于拇指在持握状态下自然摆动指腹所经过的所述操控手柄2上的曲面。所述起止键21凸出位置可以设置在所述操控手柄2的持握段的偏左面，以利于右利手者使用，也可以设置在所述操控手柄2的持握段的偏右面，以利于左利手者使用，还可以设置在所述操控手柄2持握段的正前面，右利手和左利手者均方便使用。优选的，所述起止键21凸出位置设置在所述操控手柄2持握段的正前面。
所述操控手柄2的面板设置一个加速键22和一个减速键23。所述加速键22和所述减速键23分别与所述按键模块43具有加速、减速功能的键通过柔性电线连接。所述加速键22和减速键23的凸出位置设置在所述操控手柄2偏左面或偏右面或正前面。优选的，所述加速键22和减速键23与所述起止键21的凸出位置设置在同一纵向曲面上，优选的，所述加速键22和减速 键23的凸出位置设置在所述操控手柄2头端面板的正前面。
所述起止键21、所述加速键22和减速键23的键帽形状、大小、材质以及凸出高度满足大部分人手指灵活操作需要，优选的，可以根据个人喜好更换不同形状、大小和材质键帽。
用持握所述电动剥卵器的手的拇指对所述起止键21、所述加速键22和所述减速键23进行控制，和/或用另一只手对所述加速键22和所述减速键23进行辅助控制。
所述连接端口24通过螺纹接口与所述操控手柄2的主体连接。所述操控手柄2通过连接端口24与所述剥卵针1形成插入式紧密连接。所述连接端口24根据其连接的所述剥卵管1不同，可以设计不同的所述连接端口24变体，如图3－1、图3－2所示的适应所述剥卵吸头111和所述剥卵针121的连接端口241，如图4－1、图4－2所示的适应剥卵巴氏管131的连接端口242。
如图3－1－1和图3－1－2所示，所述连接端口241的中央纵轴为中央孔2411，所述中央孔2411的端口末端缩小形成一个限位环2412。所述中央孔2411容纳一个密封管251，所述密封管251为弹性材质，如橡胶、乳胶、硅胶等。通过所述限位环2412限位在所述中央孔2411内，所述密封管251外壁与所述中央孔2411内壁紧密贴合，具有严格气密性。所述密封管251于所述限位环2412的一端的开口具有与所述剥卵针121形成插入式紧密连接的接口2511，所述端口241具有与所述剥卵吸头111针座形成插入式紧密连接的接头2413。所述密封管251于所述限位环2412端对侧的开口具有与连通所述微型缸筒3111的软管或与所述微型缸筒3111形成紧密连接的接口2512，还具有与连通吸头8的软管形成紧密连接的接口2513，和/或具有与连接压力感应器10的软管形成紧密连接的接口2514。
如图4－1－1和图4－1－2所示，所述连接端口242的中央纵轴为中央孔2421，中央孔2421的端口末端缩小形成一个限位环2422。所述中央孔2421容纳一个密封管252，所述密封管252为弹性材质，如橡胶、乳胶、硅胶等，通过所述限位环2422限位在所述中央孔2421内，所述密封管252外壁与所述中央孔2421内壁紧密贴合，具有严格气密性。所述密封管252于所述限位环2422的一端的开口具有与所述剥卵巴氏管131尾端形成插入式紧密连接的 接口2521。所述密封管252于限位环端对侧的开口具有与连通微型缸筒311的软管或与所述微型缸筒311形成紧密连接的接口2522，还具有与连通吸头8的软管形成紧密连接的接口2523，和/或具有与连接压力感应器10的软管形成紧密连接的接口2524。
所述动力模块3包括微缸筒模块31、滑槽32、所述传动机构33、所述步进电机34。
如图5－1－1和5－1－2所示，所述微缸筒模块31包括微型缸筒311、微型推杆312、和/或密封管313、和/或压紧件314和/或压紧螺帽315。所述微型缸筒311可以为简单圆管状结构的微型缸筒311－1，也可以为在所述微型缸筒311－1基础上改进的微型缸筒311－2。如图5－2所示，所述微型推杆312的外壁和所述微型缸筒311－1可以组成结构简单的所述微缸筒模块31－1，所述微型推杆312的外壁和所述微型缸筒311－1的内壁在具有严格气密性的同时具有小的摩擦力。如图5－1所示，所述微型缸筒311－1的所述微型推杆312端的中央孔内径增大形成一段圆形腔，对应的外壁具有螺纹形成所述微型缸筒311－2；所述密封管313为弹性材质，如橡胶、乳胶、硅胶等，其中央孔内径与所述微型推杆312外径相同，其外径与所述微型缸筒311圆形腔的内径相同；所述压紧件314为中空的金属圆柱体，包括粗段和细段，其中央孔内径大于所述微型推杆312外径，其粗段的外径与所述微型缸筒311－2圆形腔的内径相同，大于所述压紧螺帽315螺纹接口对侧端的圆形开口的内径，其细段的外径等于所述压紧螺帽315螺纹接口对侧端的圆形开口的内径；所述压紧螺帽315为中空的异形螺帽，具有一端的螺纹开口及其对侧端的圆形开口。所述密封管313容纳于所述微型缸筒311－2的圆形腔，所述压紧件314粗段紧贴所述密封管313，所述压紧螺帽315与所述微型缸筒311－2的螺纹连接后，所述压紧件314的粗段及部分细段位于所述压紧螺帽315内，细段余下部分伸出所述压紧螺帽315的圆形开口。如图5－2所示，所述微型推杆312经所述压紧件314的中央孔、所述密封管313中央孔插入所述微型缸筒311－2内，组成所述微缸筒模块31－2。所述压紧螺帽315通过所述压紧件314压迫所述密封管313使之变形，通过调节所述压紧螺帽315与所述微型缸筒311－2螺纹连接的螺纹高度，可以调节所述密封管313的径向变形程度，进 而调节所述密封管313与所述微型推杆312的气密性及摩擦力。
如图5－2和图5－3所示，所述动力模块3中的所述传动机构33根据原理进行以下两种设置：以丝杆3311、螺母－滑块3312为要传动部件的传动机构331，以直齿轮3321、直齿条－滑块3322为主要传动部件的传动机构332。选择其中一种作为所述电动剥卵器的传动机构。
如图5－2所示，在所述传动机构331结构中，螺母和滑块一体化设计，所述丝杆3311为所述步进电机34转轴的输出段；所述滑槽321既可以固定所述步进电机34，又可以固定所述微缸筒模块31；所述微型推杆312的一端固定在所述螺母－滑块3312上。整体上，所述步进电机34、所述滑槽321、所述螺母－滑块3312和所述微缸筒模块31构成所述动力模块3－1，既方便组装又节省空间。
如图5－3所示，在所述传动机构332结构中，直齿条和滑块一体化设计，所述直齿轮3321组装在所述步进电机34转轴的输出段；所述滑槽322既可以固定所述步进电机34，又可以固定所述微缸筒模块31；所述微型推杆312的一端固定在所述直齿条－滑块3322上。整体上，所述步进电机34、所述滑槽322、所述直齿条－滑块3322和所述微缸筒模块31构成所述动力模块3－2，既方便组装又节省空间。
所述传动机构33的作用是将所述步进电机34的正转、反转转换成所述微型推杆312直线往返运动。在所述动力模块3－1中，所述步进电机34的步距角和所述丝杆3411的螺距决定了所述步进电机34单个脉冲的直线行程；在所述动力模块3－2中，所述步进电机34的步距角和所述直齿轮3421的分度圆直径决定了所述步进电机34单个脉冲的直线行程。所述传动机构33的物理参数如丝杆螺距、导程、直齿轮分度圆直径与所述步进电机34步距角能够满足剥卵程序调节液柱长度需要。
所述动力模块3中的所述步进电机34优选2相四线步进电机，为了使所述电动剥卵器更加轻巧，在能够满足执行剥卵程序的前提下，优选体积更小、能耗更低的所述步进电机34。
所述动力模块3设置在所述操控手柄2的所述动力模块仓26内，在所述分体式电动剥卵器中，所述动力模块3还可以设置在控制盒11内。
所述控制模块4包括步进电机驱动器41、单片机42、按键模块43。所述步进电机驱动器41、所述单片机42和所述按键模块43可以采用集成化电路模块。
所述按键模块43至少包括具有程序选择、起止、加速、减速、增加行程、减少行程功能的键或电连接点。优选的，不同功能可以组合在一个键上实现，如，同一按键相依次的两次按压可以执行不同的功能，同一按键按压持续时间不同可以执行不同的功能，同一按键连续按压的次数不同可以执行不同的功能等。
所述按键模块43的键可以是按钮按键或触屏按键等。
所述显示模块5包含显示屏51、按键模块52。所述显示屏51用于显示运行程序的基本参数，如程序号、吹吸频率、液柱长度，和/或运行数据，如实时吹吸频率、某吹吸频率起止时间、某吹吸频率累积操作时间、总操作时间，和/或电池或蓄电池电量，和/或警报等信息。所述显示屏51为LED或LCD显示屏或触摸显示屏，支持多语种显示，语言至少包括中文、英文等。可以为黑白或者彩色显示屏。
所述显示屏51安装在所述操控手柄2头端面板的正前面或者持握的手的拇指所在侧的所述操控手柄2头端面板的侧面和/或所述控制盒11的正前面。
根据安装位置的不同，选择合适尺寸和分辨率的所述显示屏51，并选择适当显示内容。如果所述显示屏51只安装在所述操控手柄2头端面板，优选具有高分辨率的显示屏，所述显示屏51的大小在满足显示运行程序的基本参数同时符合所述电动剥卵器整体上轻巧美观的要求。
如果所述控制手柄2和所述控制盒11上都设置所述显示模块5，优选的，所述控制手柄2上的所述显示屏51只需要尺寸更小、分辨率相对低的显示屏。而所述控制盒11上的所述显示屏51需要尺寸更大、分辨率更高的显示屏，满足操作者1米视距内在阴暗视场环境下清晰辨识显示屏内容的需要。在能够满足上述条件的前提下，优选能耗更低的所述显示屏51。
所述按键模块52的键可以是按钮按键或触屏按键等。所述按键模块52所具功能与所述显示器51相适应，优选的，部分键和所述控制模块4的所述按键模块43的键合并，使键的数量尽可能的少，操作界面更加简约。
所述电源模块6包含充电模块61和电能存储单元62。所述充电模块61对所述电能存储单元充电。电能存储单元为蓄电池或者为类似锂离子电池的电池。在能满足电动剥卵器正常用电需求的前提下，优选体积更小重量更轻的所述充电模块61和电能存储单元62。
所述电源模块6设置在所述操控手柄2的所述主仓27内或所述控制盒11内。
所述内存7用于存储程序运行数据，包括操作对象信息，如编号等，及该操作对象相对应的剥卵操作数据，如程序号、吹吸频率、液柱长度和程序运行数据，如实时吹吸频率、某吹吸频率起止时间、某吹吸频率累积操作时间、总操作时间等数据。所述内存7如与所述控制模块4电连接，设置在所述操控手柄2的壳体内或所述控制盒11内。
所述吸头8呈圆柱形或者类球形，为弹性材质，如橡胶、乳胶、硅胶等。所述吸头8设置在所述操控手柄2的持握段上，连接端埋于所述操控手柄2面板下，盲端暴露在外面，并以一定角度，比如30度，向所述操控手柄2尾端倾斜。所述吸头8连接端通过管道与所述剥卵管1相连通。所述吸头8具体位置与所述操控手柄2上其他操作元件，如所述起止键21、所述加速键22和所述减速键23配合设置。优选的，所述吸头8与所述起止键21相邻，沿所述操控手柄2纵向分布，所述吸头8暴露部分位于握持所述电动剥卵器的手的拇指的外侧端，拇指仅通过自然左右摆动即可对所述起止键21和所述吸头8实现变换操作。拇指在常规操作姿势下按压所述吸头8形成的最大体积变量为所述吸头8的有效排空体积，有效排空体积小于所述剥卵针12或所述剥卵吸头111的容积。通过操控所述吸头8，可以对卵母细胞进行转移等操作。
所述电动剥卵器还可以设置语音模块9。剥卵操作时，操作者双眼或者单眼靠近显微镜目镜观察剥卵过程，为了避免剥卵过程中转移视线去观察显示屏上的信息而中断剥卵操作，可以将显示屏上的某些信息如实时吹吸频率等采用声音编码，通过扬声器播放，操作者通过声音获取该信息；剥卵操作，属于合子胚胎体外操作，有严格的体外操作时间限制。通过单片机编程，可以将总操作时间和/或超时提醒通过语音形式告知操作者。
所述语音模块9设置在所述操控手柄2的所述主仓27内或所述控制盒11内。
所述电动剥卵器还可以设置压力传感器10。通过所述压力传感器10监测所述微型推杆312与所述剥卵管1之间的连通管道的压力变化，将压力信息提交给单片机系统，单片机系统根据压力变化值自动调整控制步进电机的脉冲频率，实现自动调速的目的。当卵冠丘复合体通过所述剥卵针1尖部被卡住而通过缓慢时，传统剥卵方法是通过增加吸力或吹力使其加速通过。每个操作者对该情形做出的判断及处理方法受主观因素影响大，不利于标准化。当卵冠丘复合体通过所述剥卵针1尖部被卡住而通过缓慢时导致连通管道内压力变低，可以测定该低压值。选用适当的低压值作为阈值下限进行编程。当所述压力传感器10检测到该阈值下限时，反馈至所述单片机42，按照程序设置自动增加控制所述步进电机34的脉冲频率到设定高值，提高吹吸频率，进而增大吸力和吹力，使卵冠丘复合体顺利通过所述剥卵管1口部。在以设定脉冲频率高值完成设定吹吸次数后恢复原有吹吸频率，从而达到自动调整吹力和吸力的目的。与传统剥卵方法调节吹吸速度比较而言，自动调节吹吸频率由所述微型推杆312与所述剥卵管1之间的连通管道的压力低于阈值下限触发，不受操作者主观因素影响，容易标准化。
所述压力传感器10设置在所述操控手柄2的壳体内。所述操控手柄2的面板上设置启动/关闭该功能的自动调速键。
所述控制盒11作为其他元件安装的载体，在上述元件与所述控制手柄2物理性分离时，分离的元件安装于所述控制盒11内，并在其面板上设置相应的按键、按钮及接口。
如图1所示，所述电动剥卵器各主要元件的连接关系如下：所述步进电机驱动器41、所述按键模块43、所述显示模块5、所述电源模块6、所述内存7、所述语音模块9、所述压力传感器10分别与单片机42连接，所述步进电机驱动器41的输出端与所述步进电机34连接；所述脚踏开关控制器12开关与所述按键模块43相应的按键电连接。
所述步进电机34的输出轴与所述传动机构33的输入端连接，所述传动机构33的输出端与微型推杆312的一端连接，所述微型推杆312的另一端置 于所述微型缸筒311内，所述微型缸筒311与所述剥卵管1通过管道相连通或通过密封管251或通过密封管252直接连通，所述吸头8通过管道与所述剥卵管1相连通，所述压力传感器10的压力检测元件与所述剥卵管1通过管道连通。所述操控手柄2通过所述连接端口24与所述剥卵管1形成插入式紧密连接。
除了所述剥卵管1与所述操控手柄2形成插入式可拆卸式紧密连接外，所述电动剥卵器的各个主要部件固定组装在所述操控手柄2内组装成一体式电动剥卵器，还可以除了所述剥卵管1与所述操控手柄2形成插入式可拆卸式紧密连接外，其余部件与所述操控手柄2物理性分离固定组装在所述控制盒11内构成分体式电动剥卵器。在所述分体式电动剥卵器中，相关部件之间通过柔性电线或柔性管道形成连接。电动剥卵器各主要元件的空间布局可以按下述五种方式设计，每种设计方式构成外形相异功能相近的电动剥卵器。
方式一：所述剥卵管1与所述操控手柄2连接端口形成插入式紧密连接，所述动力模块3、所述控制模块4、所述显示模块5、所述电源模块6、所述内存7、所述吸头8、所述语音模块9、所述压力传感器10安装在所述操控手柄2内，形成一体式的所述电动剥卵器。
方式二：所述剥卵管1与所述操控手柄2连接端口形成插入式紧密连接，所述动力模块3、所述控制模块4、所述显示模块5、所述内存7、所述吸头8、所述语音模块9、所述压力传感器10安装在所述操控手柄2内，所述电源模块6安装在所述控制盒11内。所述控制盒11还可以安装一个所述显示模块，该所述显示模块通过柔性电线与所述控制模块4连接。所述控制盒11与所述控制手柄2物理性分离，形成分体式的所述电动剥卵器。还可以设置一个与上述元件物理性分离的脚踏开关控制器12，在该情形下，所述操控手柄2面板上的所述起止键21、所述加速键22和所述减速键23可以保留或者取消。
方式三：所述剥卵管1与所述操控手柄2连接端口形成插入式紧密连接，所述动力模块3、所述显示模块5、所述吸头8、所述压力传感器10安装在所述操控手柄2内，所述控制模块4、所述电源模块6、所述内存7和所述语音模块9安装在所述控制盒11内。所述操控手柄2内的所述显示模块5通过 柔性电线与所述控制模块4连接。所述控制模块4的所述按键模块的部分键，如控制起止、加速及减速功能的键，通过柔性电线与所述操控手柄2的起止键、加速键及减速键连接。所述压力传感器10的压力检测元件通过柔性电线与所述控制模块4连接。所述控制盒11与所述控制手柄2物理性分离，形成分体式的所述电动剥卵器。还可以设置一个与上述元件物理性分离的脚踏开关控制器12，在该情形下，所述操控手柄2面板上的所述起止键21、所述加速键22和所述减速键23可以保留或者取消。
方式四：所述剥卵管1与所述操控手柄2连接端口形成插入式紧密连接，所述显示模块5、所述吸头8、所述压力传感器10安装在所述操控手柄2内，所述动力模块3、所述控制模块4、所述电源模块6、所述内存7和所述语音模块9安装在所述控制盒11内。所述操控手柄2内的所述显示模块5通过柔性电线与所述控制模块4连接。所述控制盒11内还可以安装一个所述显示模块，该所述显示模块通过柔性电线与所述控制模块4连接。所述控制模块4的所述按键模块的部分键，如控制起止、加速及减速功能的键，通过柔性电线与所述操控手柄2的起止键、加速键及减速键连接。所述动力模块3中的所述微型缸筒311通过柔性管道与所述剥卵管1相连通，在所述微型推杆312与所述剥卵管1之间的连通管道填充液体，如矿物油。所述压力传感器10的压力检测元件通过柔性电线与所述控制模块4连接。所述控制盒11与所述控制手柄2物理性分离，形成分体式的所述电动剥卵器。还可以设置一个与上述元件物理性分离的脚踏开关控制器12，在该情形下，所述操控手柄2面板上的所述起止键21、所述加速键22和所述减速键23可以保留或者取消。
方式五：所述剥卵管1与所述操控手柄2连接端口形成可拆卸式紧密连接，所述吸头8、所述压力传感器10安装在所述操控手柄2内，所述动力模块3、所述控制模块4、所述显示模块5、所述电源模块6、所述内存7和所述语音模块9安装在所述控制盒11内。所述控制模块4的所述按键模块的部分键，如控制起止、加速及减速功能的键，通过柔性电线与所述操控手柄2的起止键、加速键及减速键连接。所述动力模块3中的所述微型缸筒311通过柔性管道与所述剥卵管1相连通，在所述微型推杆312与所述剥卵管1之间的连通管道填充液体，如矿物油。所述压力传感器10的压力检测元件通过 柔性电线与所述控制模块4连接。所述控制盒11与所述控制手柄2物理性分离，形成分体式的所述电动剥卵器。还可以设置一个与上述元件物理性分离的脚踏开关控制器12，在该情形下，所述操控手柄2面板上的所述起止键21、所述加速键22和所述减速键23可以保留或者取消。
采用本发明的电动剥卵装置的剥卵方法如下：ICSI前或者冻卵前剥卵，用未拉制的巴氏管将卵冠丘复合体转移入含80IU/L的透明质酸酶溶液内，连续吹打数次，去除大部分粘液块，然后将预消化的卵冠丘复合体转移到操作培养基内，由步进电机34提供吹吸动力使预消化的卵冠丘复合体反复进出剥卵管嘴部，直到去除颗粒细胞程度达到操作要求；
采用本发明的电动剥卵装置的剥卵方法还可以如下：试管法IVF后剥卵，用未拉制的巴氏管将试管法IVF后的卵冠丘复合体培养基悬液转移至空的培养皿，由步进电机提供吹吸动力使卵冠丘复合体反复进出剥卵管嘴部，直到去除颗粒细胞程度达到操作要求；微滴法IVF后剥卵，由步进电机34提供吹吸动力反复吹吸随颗粒细胞附壁在培养皿底的卵母细胞，使之从皿底脱落，再经反复吹吸直到去除颗粒细胞程度达到操作要求。
所述步进电机34由单片机42控制。单片机42内设有单片机程序，程序的参数主要包括吹吸频率、液柱长度。由步进电机34行程调节吹吸液柱长度。在所述液柱长度一定的条件下，由步进电机34往返频率调节吹吸频率，相应的调节吹吸速度及对应的吹吸力量，吹吸频率越快，速度越快，吹吸力量也越大。单片机程序可以随时修改和/或存储为新的程序，可以随时启动、停止。
所述步进电机34至少包括三种调速方式：交替使用预存程序调速、在运行程序吹吸频率的基础上手动增加或降低单次幅度可以通过编程设定的频率值、在运行程序的吹吸频率的基础上自动加速至某一设定吹吸频率高值并持续至设定时间后恢复原有吹吸频率。
剥卵操作数据记录于内存，可以查看和/或输出。剥卵操作数据主要包括：运行程序的基本参数，如程序号、吹吸频率、液柱长度和运行数据，如实时吹吸频率、某吹吸频率起止时间、某吹吸频率累积操作时间、总操作时间等数据。
以上的具体实施方式仅为本创作的较佳实施例，并不用以限制本创作， 凡在本创作的精神及原则之内所做的任何修改、等同替换、改进等，均应包含在本创作的保护范围之内。

Claims (16)

  1. 一种电动剥卵装置，其特征在于：包括剥卵管，操控手柄，安装于操控手柄内的动力模块、控制模块、电源模块和内存以及吸头；其中，所述操控手柄的一端设有连接端口，所述剥卵管连接于连接端口上；所述动力模块包括微缸筒模块、传动机构以及步进电机；所述控制模块包括步进电机驱动器、单片机以及按键模块；所述步进电机驱动器、按键模块、显示模块、电源模块、内存分别与所述单片机连接；所述步进电机驱动器与所述步进电机连接；所述步进电机与所述传动机构连接，所述传动机构与一微型推杆连接；所述微型推杆的另一端置于所述微型缸筒内，所述微型缸筒与所述剥卵管密封管连通；所述吸头与所述剥卵管相连通。
  2. 如权利要求1所述的电动剥卵装置，其特征在于：所述剥卵管具体为剥卵吸头、剥卵针或剥卵巴氏管。
  3. 如权利要求1所述的电动剥卵装置，其特征在于：于所述操控手柄上设置有起止键、加速键和减速键，该起止键、加速键和减速键分别和按键模块电性连接。
  4. 如权利要求3所述的电动剥卵装置，其特征在于：于所述操控手柄内设有动力模块仓和主仓；所述动力模块收容于动力模块仓内；所述控制模块、电源模块收容于主仓内；于所述操控手柄上还嵌设有一显示屏。
  5. 如权利要求3所述的电动剥卵装置，其特征在于：所述连接端口的中央设有一中央孔，所述中央孔的端口末端缩小形成一个限位环；所述中央孔容纳一个密封管，所述限位环限位在所述中央孔内，所述密封管外壁与所述中央孔内壁紧密贴合；所述密封管的一端的开口具有与所述剥卵管形成插入式紧密连接的接口；所述密封管的另一端开口具有与连通微型缸筒形成紧密连接的接口，与连通吸头形成紧密连接的接口，和与连接压力感应器形成紧密连接的接口。
  6. 如权利要求1所述的电动剥卵装置，其特征在于：所述微缸筒模块包括微型缸筒、密封管、压紧件以及压紧螺帽；其中，所述微型推杆和微型缸筒连接；所述密封管容纳于所述微型缸筒内，所述压紧件紧贴所述密封管，所述压紧螺帽与所述微型缸筒的螺纹连接，其一部分位于所述压紧螺帽内， 另一部分伸出所述压紧螺帽的圆形开口。
  7. 如权利要求1所述的电动剥卵装置，其特征在于：所述传动机构包括丝杆和螺母－滑块；其中，所述丝杆连接所述步进电机；所述微型推杆的一端固定在所述螺母－滑块上。
  8. 如权利要求1所述的电动剥卵装置，其特征在于：所述传动机构包括直齿轮和直齿条－滑块；其中，所述直齿轮组装在所述步进电机上；所述微型推杆的一端固定在所述直齿条－滑块上。
  9. 如权利要求1所述的电动剥卵装置，其特征在于：其还设有一语音模块，所述语音模块与所述单片机连接。
  10. 如权利要求1所述的电动剥卵装置，其特征在于：其进一步可包括一控制盒，所述控制盒通过线缆连接至操控手柄上。
  11. 如权利要求10所述的电动剥卵装置，其特征在于：于所述控制盒上设有一显示屏；或者在所述操控手柄和控制盒上分别设有一显示屏。
  12. 如权利要求1所述的电动剥卵装置，其特征在于：其还可包括一压力传感器，该压力传感器亦与单片机连接，该压力传感器的压力检测元件与所述剥卵管通过管道连通。
  13. 一种采用如权利要求1所述的电动剥卵装置的剥卵方法，其特征在于：包括如下步骤：用未拉制的巴氏管将卵冠丘复合体转移入透明质酸酶溶液内，连续吹打数次，去除大部分粘液块，然后将预消化的卵冠丘复合体转移到操作培养基内，由步进电机提供吹吸动力使预消化的卵冠丘复合体反复进出剥卵管嘴部，直到去除颗粒细胞程度达到操作要求。
  14. 如权利要求13所述的剥卵方法，其特征在于：所述步进电机由单片机控制，其至少包括三种调速方式：交替使用预存程序调速、在运行程序吹吸频率的基础上手动增加或降低单次幅度可以通过编程设定的频率值、在运行程序吹吸频率的基础上自动加速至某一设定吹吸频率高值并持续至设定时间后恢复原有吹吸频率。
  15. 一种采用如权利要求1所述的电动剥卵装置的剥卵方法，其特征在于：包括如下步骤：用未拉制的巴氏管将试管法IVF后的卵冠丘复合体培养基悬液转移至空的培养皿，由步进电机提供吹吸动力使卵冠丘复合体反复进 出剥卵管嘴部，直到去除颗粒细胞程度达到操作要求；微滴法IVF后剥卵，由步进电机提供吹吸动力反复吹吸随颗粒细胞附壁在培养皿底的卵母细胞，使之从皿底脱落，再经反复吹吸直到去除颗粒细胞程度达到操作要求。
  16. 如权利要求15所述的剥卵方法，其特征在于：所述步进电机由单片机控制，其至少包括三种调速方式：交替使用预存程序调速、在运行程序吹吸频率的基础上手动增加或降低单次幅度可以通过编程设定的频率值、在运行程序吹吸频率的基础上自动加速至某一设定吹吸频率高值并持续至设定时间后恢复原有吹吸频率。
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