WO2015191429A1 - Ensemble flacon d'incubateur pour culture cellulaire - Google Patents

Ensemble flacon d'incubateur pour culture cellulaire Download PDF

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Publication number
WO2015191429A1
WO2015191429A1 PCT/US2015/034646 US2015034646W WO2015191429A1 WO 2015191429 A1 WO2015191429 A1 WO 2015191429A1 US 2015034646 W US2015034646 W US 2015034646W WO 2015191429 A1 WO2015191429 A1 WO 2015191429A1
Authority
WO
WIPO (PCT)
Prior art keywords
flask
base element
opening
cross sectional
vessel
Prior art date
Application number
PCT/US2015/034646
Other languages
English (en)
Inventor
Noah B. PEFAUR
Original Assignee
Abbvie, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbvie, Inc. filed Critical Abbvie, Inc.
Publication of WO2015191429A1 publication Critical patent/WO2015191429A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02—Filters

Definitions

  • Embodiments of the present invention relate generally to devices and apparatus for cell culture.
  • Laboratory equipment for performing cell culture for example, cell culture shaking apparatus is expensive and often limited to specialized consumable articles, due to the size and/or shape of features that receive such articles.
  • One such specialized consumable article is a multiple well plate.
  • the typical multiple well plate has a base with dimensions of 12.8 centimeters by 8.6 centimeters and a height of approximately 3.5 to 4.5 centimeters.
  • a deep well multiple well plate has the same base dimensions and a height of approximately 2.5 to 4.0 centimeters.
  • Typical multiple well microtiter plates are not suited for larger scale cell culture because the wells are often too small, leading to reduced growth, viability, and protein production levels when compared to cell cultures grown in shake flasks.
  • certain equipment for receiving such multiple plates such as incubators, shakers, robotic liquid handling systems, and samplers cannot use, flasks, vials and the like because they often lack the ability to grasp rounded objects, or orient those objects on a platform.
  • These physical constraints impede the ability to automate scale up production of cell cultures on robotics platforms, and process cell culture products once produced.
  • the use of flasks, vials, etc., for cell culture places constraints on throughput, as stacking flasks in a shaking incubator is impractical due to their footprint, shape, and height.
  • Embodiments of the present invention are directed to devices and methods for performing cell culture in equipment for receiving multiple well plates where a larger volume of cell culture is required.
  • the term "cell culture” refers to the process of growing cells outside their natural environment and, in particular, to growing eukaryotic cells of multicellular organisms under controlled conditions.
  • One embodiment of the device of the present invention has a flask element and a base element.
  • the flask element has one or more walls defining a vessel having an opening, interior bottom surface and one or more interior side surfaces and at least one exterior surface. At least one of the interior bottom surface and the interior side surface is constructed and arranged to support a cell culture.
  • the vessel has a volume of greater than about 25 milliliters.
  • the opening has a cross sectional area and the interior bottom surface has a cross sectional area and the cross sectional area of the interior bottom surface is greater than the cross sectional area of the opening.
  • the base element is affixed to the exterior surface of the flask element to hold the flask element in an operating position within an incubator shaking device.
  • the base element is constructed and arranged to have a footprint corresponding to a multiple well plate, with the base element and flask element constructed and arranged, in an embodiment, to have a height not exceeding a deep well multiple well plate.
  • the flask element affixed to the base element is for containing cell cultures in shaking type incubators designed for multiple well plates.
  • the base element has dimensions of approximately
  • the term “footprint of a multiple well plate” refers to such dimensions. And, the flask element and the base element have a total height of about 3.5 to 4.5 centimeters. As used herein, the term “height of a deep well multiple well plate” refers to such dimensions. These dimensions approximate the dimensions of 96 well plates and multiples of 96 well plates. As used herein, the term “affixed” means attached to or held by and also means incorporated as a unitary structure therewith.
  • One further device features a flask element having one or more walls defining a vessel having an opening, bottom surface, one or more interior side surfaces and an exterior surface section and a frusto-conical section with a top opening. At least one of the interior bottom surface and the interior side surface is constructed and arranged to support a cell culture.
  • One embodiment of the flask element features an exterior surface that is constructed and arranged to cooperate with a base element. That is, the exterior surface is received by a base element to hold the flask in operating position within a multiple well device.
  • One embodiment features a flask element that is constructed and arranged to be removably affixed to the base element.
  • the flask element can be removed from the base element for special processing. For example, it may be desirable to autoclave or sterilize the flask element apart from the base element.
  • One embodiment of the flask element features an exterior surface that cooperates with tab elements on the base element which tab elements hold the flask element in the operating position.
  • the flask element may be affixed to one or more base elements.
  • One embodiment of the flask element features a bottom surface having a depression for collecting fluids.
  • the fluids collected in the depression are extracted by extracting tools such as pipettes and the like which can enter the opening and extend into the depression.
  • One embodiment of the flask element features a bottom surface having a circular form with a diameter of about eight centimeters.
  • the opening, having a cross sectional area less than the cross sectional area of the interior bottom surface is about 30% of the diameter of the interior bottom surface, or about 1.0 to 2.5 centimeters.
  • the at least one side surface is generally cylindrical, although other shapes may be used, having a height of about 1.0 to about 3.0 centimeters.
  • One embodiment features at least one wall having a frusto-conical section having an opening.
  • One embodiment features a frusto-conical section having a height of about 1.0 to about 3.0 centimeters.
  • the total working volume of the sections of the flask defined by the one or more interior surface and bottom surface is about 25 to about 150 milliliters.
  • a further embodiment features a total working volume of about 40 to about 60 milliliters.
  • One embodiment features a breathable filter which is interposed between the interior of the vessel at or about the opening and the exterior of the vessel for the exchange of gases and to isolate cell cultures contained by the vessel from contaminants.
  • a breathable filter is a breathable tape affixed to the exterior surface of the vessel.
  • One embodiement features raised baffles in the base of the flask portion.
  • One embodiment features a flask element or portion thereof integral to the base element as in integrally molded as a single piece or permanently fixed to the each other by fusion or glues or interfitting locking tabs, snap fittings and the like.
  • a further embodiment features a base element.
  • One embodiment features a base element having a housing for affixing to the exterior wall of a flask element to hold the flask element in an operating position within an incubator shaking device.
  • the base element constructed and arranged to have a footprint corresponding to a multiple well plate and the base element and flask element constructed and arranged to have a height not exceeding a deep well multiple well plate.
  • the base element is for holding a flask element containing a cell culture affixed in an operating position in shaking type incubators designed for multiple well plates.
  • One embodiment features a housing comprised of a molded plastic, cellulosic materials, or metal frameworks and combinations thereof.
  • metal frameworks comprise racks and wire structures or plate-like metal forms.
  • Cellulosic materials comprise cardboard and the like.
  • One embodiment of a housing made of cellulosic materials is disposable.
  • One embodiment of a housing has a substantially flat folded nonworking storage position and an expanded working position for holding a flask element.
  • One embodiment of the base element features a housing having one or more side walls and one or more mounting walls or finger elements projecting inward from said side walls for holding a flask element.
  • One embodiment features a base element with cooperating top and bottom features to allow the base elements to be stacked in an interfitting assembly structure.
  • a further embodiment of the invention is directed to a method of performing cell culture.
  • the method comprises the steps of providing a device comprising a flask element and a base element.
  • the flask element has one or more walls defining a vessel having an opening, interior bottom surface and at least one interior side surface and at least one exterior surface.
  • the opening has a cross sectional area and the interior bottom surface has a cross sectional area and the cross sectional area of the interior bottom surface is greater than the opening cross sectional area.
  • At least one of the interior bottom surface and the interior side wall is constructed and arranged to support a cell culture.
  • the vessel has a volume of about 25 to 150 milliliters.
  • the base element is affixed to the exterior surface of the flask element to hold the flask element in an operating position within an incubator shaking device.
  • the base element is constructed and arranged to have a footprint corresponding to a multiple well plate, with the base element and flask element constructed and arranged to have a height not exceeding a deep well multiple well plate.
  • the flask element is affixed to the base element for containing cell cultures in shaking type incubators designed for multiple well plates, and the method further comprises the step of growing a cell culture on at least one of the interior bottom surface and interior side surface.
  • One embodiment of the present invention features the further step of placing a breathable filter element over the opening, for example, without limitation, one breathable filter element is a breathable tape.
  • Embodiments of the present method feature the use of the device received in an operating position in processing equipment for multiple well plates. Further embodiment feature the exchange, or replacement of a first flask element with a second flask element or a first flask element that has been processed apart from the base element.
  • Figure 1 depicts a cutaway plan view of a device embodying features of the present invention.
  • Figure 2 depicts a top view of a device embodying features of the present invention.
  • FIG. 3 depicts a slightly elevated exploded view of a device embodying features of the present invention. Detailed Description of the Invention
  • Embodiments of the present invention will be described in detail with respect to devices and methods for performing cell culture in equipment for receiving multiple well plates, and in particular, 96 well plates. Those skilled in the art will recognize that the description herein can be modified and altered and therefore the present description should not considered limiting but as an exemplification of the present invention.
  • FIG. 1 a device, generally designated by the numeral 11 , embodying features of the present invention is depicted.
  • the device 11 has two major elements, a flask element 15 and a base element 17.
  • the flask element 15 has one or more walls 21 defining a vessel 23 having an opening 25, interior bottom surface 27 and one or more interior side surfaces 29 and at least one exterior surface 31.
  • the opening 25 has a cross sectional area and the interior bottom surface 27 has a cross sectional area; and the cross sectional area of the interior bottom surface 27 is greater.
  • At least one of the interior bottom surface 27 and the interior side surface 29 is constructed and arranged to support a cell culture. The transition from bottom surface 27 to side surface 29 may be gradual or markedly different. As depicted, the bottom surface 27 and side surface 29 has an interior edge 33.
  • Interior bottom surface 27 has a depression 35 for collecting fluids.
  • Depression 35 may comprise an indentation or, as depicted, slope in the interior bottom surfaces 27 which form a low point in the middle of the flask element 15.
  • the fluids collected in the depression 35 are extracted by extracting tools such as pipettes and the like [not shown] which can enter the opening 25 and extend into the depression 35.
  • the at least one wall 21 defining vessel 23 has a cylindrical section 39 and a frusto- conical section 41 forming part of the interior side surfaces 29 extending to the opening 25.
  • the side wall 21 can be cylindrical with a top substantially planar wall [not shown] having an opening 25 or all frustoconcial.
  • the frusto-conical section 41 has a height of about 1.0 to about 3.0 centimeters.
  • the cylindrical section 39 of side wall 21 has a height of about one to three centimeters.
  • a further embodiment features a total working volume of about 40 to about 60 milliliters.
  • the one or more walls 21 define a vessel 23 having a volume of greater than about
  • the base element 17 may comprise a wire or plate-like form or, as depicted, a housing 51 affixed to the exterior surface 31 of the flask element 15 to hold the flask element 15 in an operating position within an incubator shaking device.
  • the housing 51 is constructed and arranged to have a footprint corresponding to a multiple well plate, with the base element 17 and flask element 15 constructed and arranged to have a height not exceeding a deep well multiple well plate.
  • the base element 17 has dimensions of approximately about 12.0 to about 13.5 centimeters in length represented by line AA and about 8.0 to about 9.0 centimeters in width as represented by line BB.
  • the term "footprint of a multiple well plate” refers to such dimensions.
  • the flask element 15 and the base element 17 have a total height of about 3.5 to about 4.5 centimeters as represented by line CC.
  • the term "height of a deep well multiple well plate” refers to such dimensions. These dimensions approximate the dimensions of 96 well plates and multiples of 96 well plates.
  • the flask element 15 has an exterior surface 31 that is constructed and arranged to cooperate with a base element 17.
  • the exterior surface 31 is received by housing 51 of a base element 17 to hold the flask element 15 in operating position within a multiple well apparatus.
  • Flask element 15 is permanently affixed to the base element 17 or in the alternative is constructed and arranged to be removably affixed to the base element 17.
  • flask element 15 and the housing 51 depicted in Figure 2 can be made as a unitary molded structure, or flask element 15 is glued or potted into a housing 51 by conventional means.
  • base element 17 has a housing 51 comprising a bottom piece 59 and a top plate 53.
  • Top plate 53 fits about the exterior surface 31 of flask element 15 about the opening 25.
  • Flask element 15 has a lip 55 which cooperates with a matching opening 57 in top plate 53.
  • Top plate 53 snap fits into bottom piece 59 to hold flask element 15 in operating position.
  • Flask element 15 can be removed from the base element 17 for special processing by opening the top plate 53 from the housing 51. For example, it may be desirable to autoclave or sterilize the flask element 15 apart from the base element 17.
  • flask element 15 may cooperate with tab elements or flanges on the base element 15 which tab elements or flanges [not shown] hold the flask element in the operating position.
  • Top plate 53 may be hinged to bottom piece 59 and fold down in the manner of a clam-shell or egg-carton.
  • the base element 17 and flask element 17 can be considered disposable or reusable apart from the other.
  • the base element 17 can be made of plastic, metals or cellulosic materials.
  • Cellulosic materials such as cardboard and polymeric materials such as expanded foam plastic (Styrofoam®) can be readily folded into a collapsed form for storing and unfolded into a more three dimensional form for holding a flask element 15 in an operating position.
  • Flask element 15 is preferably made of glass or plastics suitable for cell culture.
  • base element 17 has a top surface 65 and, in Figure 2, a bottom surface 67.
  • the bottom surface 67 has legs or pins 69, which are received in cooperating holes 71 in the top surface 65 when one base element 17 is placed on top of another base element 17 to provide a stable stack.
  • Other features similar to the cooperating pin and hole described can be readily substituted, such as cooperating lip and rim and the like.
  • a breathable filter is interposed between the interior of the vessel 23 at or about the opening 25 and the exterior of the vessel 31 for the exchange of gases and to isolate cell cultures contained by the vessel from contaminants.
  • One breathable filter is a breathable tape 61 affixed to the exterior surface 31 at the opening 25 or across the lip 55 of flask element 15 as seen in Figure 3.
  • the flask element 15 and the base element 17 of device 11 have been described as such relate to each other.
  • the manner of using the device 11 will be described as a method of performing cell culture.
  • the method comprises the steps of providing a device 11 comprising a fiask element 15 and a base element 17.
  • the term "providing” means having in one's possession to start the process.
  • the fiask element 15 has one or more walls 21 defining a vessel 23 having an opening 25, interior bottom surface 27 and one or more interior side surfaces 29 and at least one exterior surface 31. At least one of the interior bottom surface 27 and the interior side surface 29 is constructed and arranged to support a cell culture.
  • the opening 25 has a cross sectional area and the interior bottom surface 27 has a cross sectional area, and the cross sectional area of the interior bottom surface 27 is greater than that of the opening 25.
  • the vessel has a volume of greater than about 25 milliliters.
  • the base element 17 is affixed to the exterior surface 31 of the flask element 15 to hold the flask element 15 in an operating position within an incubator shaking device.
  • the base element 17 is constructed and arranged to have a footprint corresponding to a multiple well plate, with the base element 17 and flask element 15 constructed and arranged to have a height not exceeding a deep well multiple well plate.
  • the flask element 15 is affixed to the base element 15 for containing cell cultures in shaking type incubators designed for multiple well plates.
  • the method further comprises the step of growing a cell culture on at least one of the interior bottom surface 27 and interior side surface 29.
  • One embodiment of the present method comprises the further step of placing a breathable filter element, such as breathable tape 61, over the opening 25.
  • a breathable filter element such as breathable tape 61
  • the device 11 is received in an operating position in processing equipment for multiple well plates.
  • the f ask element 15 is separated from the base element 17 where it is not permanently affixed.
  • the flask element 15 and the base element 17 are further processed.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Des modes de réalisation de l'invention concernent un dispositif ayant un élément de base et un élément flacon qui a un volume supérieur à environ 25 ml pour cultiver des cultures cellulaires, le dispositif étant adapté à un équipement de traitement standard à parois multiples.
PCT/US2015/034646 2014-06-09 2015-06-08 Ensemble flacon d'incubateur pour culture cellulaire WO2015191429A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462009378P 2014-06-09 2014-06-09
US62/009,378 2014-06-09

Publications (1)

Publication Number Publication Date
WO2015191429A1 true WO2015191429A1 (fr) 2015-12-17

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PCT/US2015/034646 WO2015191429A1 (fr) 2014-06-09 2015-06-08 Ensemble flacon d'incubateur pour culture cellulaire

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WO (1) WO2015191429A1 (fr)

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5577837A (en) * 1995-05-19 1996-11-26 Forma Scientific, Inc. Temperature controlled HEPA-filtered console shaker
JP2949011B2 (ja) * 1993-08-05 1999-09-13 株式会社クボタ 容器内の液体振り混ぜ装置
US20050254055A1 (en) * 2004-01-20 2005-11-17 Hong Peng Apparatus and method for monitoring biological cell culture
US20050277188A1 (en) * 2004-06-09 2005-12-15 Ellis Samuel A Fermentation flask for cultivating microorganisms
US20060166358A1 (en) * 1997-03-12 2006-07-27 University Technologies International, Inc. Biofilm incubation
WO2006138143A1 (fr) * 2005-06-15 2006-12-28 Amprotein Corporation Flacon pour culture en suspension
DE102005036763A1 (de) * 2005-08-04 2007-02-08 Inheco Industrial Heating And Cooling Gmbh System aus mehreren Inkubatoren
EP1854871A1 (fr) * 2006-05-09 2007-11-14 Infors AG Armoire tout comme procédé de traitement
WO2008060521A1 (fr) * 2006-11-14 2008-05-22 Acme Biosystems, Llc Appareil de culture cellulaire et procédés associés
EP1932904A2 (fr) * 2006-12-15 2008-06-18 Hitachi, Ltd. Appareil de culture cellulaire et son procédé de commande
EP2639294A1 (fr) * 2012-03-15 2013-09-18 CellProthera Automate et procédé automatisé de culture cellulaire
WO2014044389A1 (fr) * 2012-09-18 2014-03-27 Spicer Consulting Ltd Photobioréacteur

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2949011B2 (ja) * 1993-08-05 1999-09-13 株式会社クボタ 容器内の液体振り混ぜ装置
US5577837A (en) * 1995-05-19 1996-11-26 Forma Scientific, Inc. Temperature controlled HEPA-filtered console shaker
US20060166358A1 (en) * 1997-03-12 2006-07-27 University Technologies International, Inc. Biofilm incubation
US20050254055A1 (en) * 2004-01-20 2005-11-17 Hong Peng Apparatus and method for monitoring biological cell culture
US20050277188A1 (en) * 2004-06-09 2005-12-15 Ellis Samuel A Fermentation flask for cultivating microorganisms
WO2006138143A1 (fr) * 2005-06-15 2006-12-28 Amprotein Corporation Flacon pour culture en suspension
DE102005036763A1 (de) * 2005-08-04 2007-02-08 Inheco Industrial Heating And Cooling Gmbh System aus mehreren Inkubatoren
EP1854871A1 (fr) * 2006-05-09 2007-11-14 Infors AG Armoire tout comme procédé de traitement
WO2008060521A1 (fr) * 2006-11-14 2008-05-22 Acme Biosystems, Llc Appareil de culture cellulaire et procédés associés
EP1932904A2 (fr) * 2006-12-15 2008-06-18 Hitachi, Ltd. Appareil de culture cellulaire et son procédé de commande
EP2639294A1 (fr) * 2012-03-15 2013-09-18 CellProthera Automate et procédé automatisé de culture cellulaire
WO2014044389A1 (fr) * 2012-09-18 2014-03-27 Spicer Consulting Ltd Photobioréacteur

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