WO2015187786A3 - Programmable nucleotide enzymes - Google Patents

Programmable nucleotide enzymes Download PDF

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Publication number
WO2015187786A3
WO2015187786A3 PCT/US2015/033915 US2015033915W WO2015187786A3 WO 2015187786 A3 WO2015187786 A3 WO 2015187786A3 US 2015033915 W US2015033915 W US 2015033915W WO 2015187786 A3 WO2015187786 A3 WO 2015187786A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide
enzymes
sequences
target binding
nucleotide enzymes
Prior art date
Application number
PCT/US2015/033915
Other languages
French (fr)
Other versions
WO2015187786A2 (en
Inventor
Carina NAMIH
Katharina WENDELSTADT
Geoffrey SIWO
Hannu Rajaniemi
Remco LOOS
Cristina Cotobal MARTIN
Tim SMALLIE
Original Assignee
Helix Nanotechnologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helix Nanotechnologies, Inc. filed Critical Helix Nanotechnologies, Inc.
Publication of WO2015187786A2 publication Critical patent/WO2015187786A2/en
Publication of WO2015187786A3 publication Critical patent/WO2015187786A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is directed to compositions and methods related to nucleotide enzymes, such as DNA Iigases. In one embodiment, principles of the present disclosure provide a composition comprising at least one or two or more nucleotide enzyme(s), wherein each of the nucleotide enzymes comprises at least a first and second target binding sequence and a catalytic sequence, wherein the first and second target binding sequences of the first and second nucleotide enzyme are substantially complementary to polynucleotide target sequences and wherein upon hybridization of polynucleotide target sequences with first and second target binding sequences of any of the nucleotide enzymes,
PCT/US2015/033915 2014-06-03 2015-06-03 Programmable nucleotide enzymes WO2015187786A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462007235P 2014-06-03 2014-06-03
US62/007,235 2014-06-03

Publications (2)

Publication Number Publication Date
WO2015187786A2 WO2015187786A2 (en) 2015-12-10
WO2015187786A3 true WO2015187786A3 (en) 2016-01-28

Family

ID=54767572

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/033915 WO2015187786A2 (en) 2014-06-03 2015-06-03 Programmable nucleotide enzymes

Country Status (1)

Country Link
WO (1) WO2015187786A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060166222A1 (en) * 2005-01-21 2006-07-27 Yi Lu Nucleic acid enzyme ligation sensor
US20070072205A1 (en) * 2005-06-09 2007-03-29 Yi Lu Nanomaterial error correction
WO2008016644A1 (en) * 2006-08-01 2008-02-07 Applera Corporation Detection of analytes and nucleic acids
US20110143338A1 (en) * 2007-04-05 2011-06-16 Johnson & Johnson Research Pty Limited Nucleic acid enzymes and complexes and methods for their use
WO2013033792A1 (en) * 2011-09-09 2013-03-14 Speedx Pty Ltd Nucleic acid enzyme substrates

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060166222A1 (en) * 2005-01-21 2006-07-27 Yi Lu Nucleic acid enzyme ligation sensor
US20070072205A1 (en) * 2005-06-09 2007-03-29 Yi Lu Nanomaterial error correction
WO2008016644A1 (en) * 2006-08-01 2008-02-07 Applera Corporation Detection of analytes and nucleic acids
US20110143338A1 (en) * 2007-04-05 2011-06-16 Johnson & Johnson Research Pty Limited Nucleic acid enzymes and complexes and methods for their use
WO2013033792A1 (en) * 2011-09-09 2013-03-14 Speedx Pty Ltd Nucleic acid enzyme substrates

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LU ET AL.: "A Ligation-Triggered DNAzyme Cascade for Amplified Fluorescence Detection of Biological Small Molecules with Zero-Background Signal", J. AM. CHEM. SOC, vol. 133, 11 June 2011 (2011-06-11), pages 11686 - 11691, XP055238250, DOI: doi:10.1021/ja203693b *

Also Published As

Publication number Publication date
WO2015187786A2 (en) 2015-12-10

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