WO2015163945A8 - Development of molecular biology tools for basidiomycete 'red' yeast, sporidiobolus pararoseus engineering - Google Patents

Development of molecular biology tools for basidiomycete 'red' yeast, sporidiobolus pararoseus engineering Download PDF

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Publication number
WO2015163945A8
WO2015163945A8 PCT/US2014/071165 US2014071165W WO2015163945A8 WO 2015163945 A8 WO2015163945 A8 WO 2015163945A8 US 2014071165 W US2014071165 W US 2014071165W WO 2015163945 A8 WO2015163945 A8 WO 2015163945A8
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WO
WIPO (PCT)
Prior art keywords
molecular biology
engineering
basidiomycete
yeast
red
Prior art date
Application number
PCT/US2014/071165
Other languages
French (fr)
Other versions
WO2015163945A1 (en
Inventor
Kirk Apt
Adam Burja
Jon Hansen
David Simpson
Ross Zirkle
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2015163945A1 publication Critical patent/WO2015163945A1/en
Publication of WO2015163945A8 publication Critical patent/WO2015163945A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C12N15/04—Fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A molecular biology toolkit including tools, systems, methods, and protocols for the metabolic engineering of Urediniomycetes, specifically Sporidiobolus pararoseus. These molecular biology tools inchides extraction of genetic material such as RNA, DNA, and protein; transformation including chromosomal integration, gene targeting, and homologous recombination; and expression of both homologous and heterologous genes.
PCT/US2014/071165 2014-04-25 2014-12-18 Development of molecular biology tools for basidiomycete 'red' yeast, sporidiobolus pararoseus engineering WO2015163945A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461984254P 2014-04-25 2014-04-25
US61/984,254 2014-04-25

Publications (2)

Publication Number Publication Date
WO2015163945A1 WO2015163945A1 (en) 2015-10-29
WO2015163945A8 true WO2015163945A8 (en) 2015-12-23

Family

ID=52424100

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/071165 WO2015163945A1 (en) 2014-04-25 2014-12-18 Development of molecular biology tools for basidiomycete 'red' yeast, sporidiobolus pararoseus engineering

Country Status (1)

Country Link
WO (1) WO2015163945A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113355250B (en) * 2021-07-21 2022-08-23 江南大学 Lipid yeast and culture method thereof
CN116286413B (en) * 2023-02-24 2024-05-28 天津科技大学 Brevibacterium roseum strain capable of coloring and producing fragrance of soy sauce and application thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2005085415A1 (en) * 2004-03-04 2007-12-13 株式会社カネカ Novel transformant and method for producing polyester using the same
DE102005029170A1 (en) * 2005-06-23 2006-12-28 Consortium für elektrochemische Industrie GmbH Expression system, useful in the production of protein, comprises a host organism of Sporidiobolus and a DNA vector containing selection marker gene, which codes for a protein
MX2012010427A (en) * 2010-03-11 2012-12-05 Dsm Ip Assets Bv Yeast strains and their uses in the production of lipids.
KR20130016268A (en) * 2010-03-11 2013-02-14 비피 바이오퓨얼즈 유케이 리미티드 Methods, biological oils, biofuels, units, and organisms related to use in compression engines
DK2718442T3 (en) * 2011-06-10 2017-08-14 Temasek Life Sciences Laboratory Ltd GENETIC MANIPULATION AND EXPRESSION SYSTEMS FOR SUBPHYLA OF PUCCINIOMYCOTINA AND USTILAGINOMYCOTINA

Also Published As

Publication number Publication date
WO2015163945A1 (en) 2015-10-29

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