WO2015162161A1

WO2015162161A1 - Lncrnas for therapy and diagnosis of cardiac hypertrophy

Info

Publication number: WO2015162161A1
Application number: PCT/EP2015/058684
Authority: WO
Inventors: Thomas Thum; Kumarswamy REGALLA; Janika VIERECK
Original assignee: Medizinische Hochschule Hannover
Priority date: 2014-04-22
Filing date: 2015-04-22
Publication date: 2015-10-29
Also published as: TR201818825T4; EP3134526A1; DK3134526T3; JP6805304B2; CN106488777A; US20220315924A1; CN117224687A; PL3467111T3; PL3134526T3; ES2706878T3; US20220348920A1; DK3467111T3; EP3134526B1; JP2017514813A; US20200165604A1; EP3467111B1; US20170183652A1; HUE057484T2; HUE041700T2; US11371043B2

Abstract

The present invention relates to a pharmaceutical composition comprising (i) a compound promoting the expression and/or the activity of one or more long non-coding RNAs (lncRNAs) selected from SEQ ID NOs 12, 8 to 11 and 13; and/or (ii) a compound inhibiting the expression and/or the activity of one or more lncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28. The present invention also relates to a compound (i) promoting the expression and/or the activity of one or more lncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13; and/or (ii) inhibiting the expression and/or the activity of one or more lncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 for use in treating or preventing cardiac hypertrophy.

Description

LncRNAs for therapy and diagnosis of cardiac hypertrophy

The present invention relates to a pharmaceutical composition comprising (i) a compound promoting the expression and/or the activity of one or more long non-coding RNAs (IncRNAs) selected from SEQ ID NOs 12, 8 to 11 and 13; and/or (ii) a compound inhibiting the expression and/or the activity of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28. The present invention also relates to a compound (i) promoting the expression and/or the activity of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13; and/or (ii) inhibiting the expression and/or the activity of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 for use in treating or preventing cardiac hypertrophy.

In this specification, a number of documents including patent applications and manufacturer's manuals are cited. The disclosure of these documents, while not considered relevant for the patentability of this invention, is herewith incorporated by reference in its entirety. More specifically, all referenced documents are incorporated by reference to the same extent as if each individual document was specifically and individually indicated to be incorporated by reference.

Large-scale analysis of mammalian transcriptomes uncovered that transcription of genomes leads to a complex proportion of RNA molecules of which only a small fraction serves as templates for protein synthesis. Several studies indicate that these non-coding RNAs (ncRNAs) have important biological functions as their protein coding counterparts and suggest that altered expression or function of ncRNAs effects cardiovascular diseases, including cardiac hypertrophy and fibrosis, coronary artery disorders, as well as myocardial infarction.

The most reflected ncRNAs in cardiovascular research are microRNAs (miRNAs, miRs). These are endogenous, single-stranded RNAs composed of approximately 20-22 nucleotides that bind other transcripts reducing the stability and/or translation of their targets. For example, it was shown that miR-21 and miR-132 induce cardiac fibrosis or hypertrophy, respectively, and that in vivo repression of these miRNAs by specific antagomiRs (being chemically engineered oligonucleotides silencing miRNAs) rescues fibrosis or hypertrophy in cardiac disease model of pressure-overload (Thum et al. Nature. 2008 456(7224):980-4.; Ucar and Gupta et al. Nat Commun. 2012 3:1078). In another study it was found that miR-24 acts as a critical regulator of angiogenesis in ischemic heart disease (Fiedler et al. Circulation. 2011 124(6):720-30).

More recent studies indicate that similar to miRNAs, long ncRNAs (IncRNAs) may also play an important role in various biological processes. LncRNAs are mRNA-like transcripts ranging from 200 nucleotides up to 100 kilobases and are classified based on their genomic distribution relative to protein-coding genes (sense to exons and/or introns, antisense, bidirectional, or intergenic). Several IncRNA transcripts are exclusively restricted to the nucleus, while others are also found in the cytoplasm. Here they interact with proteins as well as other RNA or DNA molecules enabling IncRNAs to influence a variety of gene regulatory mechanisms including chromatin modification, genomic imprinting, nuclear compartmentalization and architecture, as well as transcriptional and post-transcriptional regulation (Schonrock et al. Circ Res. 2012 Oct 26;111(10):1349-62.; Caley et al. ScientificWorldJournal. 2010 10:90-102). Not surprisingly, IncRNAs are involved in human disease, such as cancer, metabolic and neuronal disorders.

However, little is known about their role in cardiovascular biology. Recent studies indicated that the two IncRNAs Braveheart (Bvht) and FOXF1 adjacent non-coding developmental regulatory RNA (Fendrr) are required for the differentiation of cardiomyocytes and the development of lateral mesoderm tissue in the heart and body wall, respectively (Klattenhoff et al. Cell. 2013 152(3):570-83.; Grote et al. Dev Cell. 2013 24(2):206-14). Both IncRNAs modulate the epigenetic profile of cells via an interaction with chromatin modifying complexes. Recent reports have also started to explore the role of IncRNAs in cardiovascular disease. Genome-wide association study (GWAS) identified single-nucleotide polymorphisms (SNPs) in loci encoding for the IncRNAs Ml AT (myocardial infarction-associated transcript) or ANRIL (antisense noncoding RNA in the INK4 locus) that seem to be related to risk of myocardial infarction or coronary artery disease (Ishii et al. J Hum Genet. 2006 51(12):1087-99.; McPherson et al. Science. 2007 316(5830): 1488-91 ). The IncRNA Kcnq1ot1 controls the expression of its antisense gene Kcnql that encodes for a potassium channel. Since the potassium channel activity is essential for a normal cardiac performance, an altered regulation related by IncRNAs might lead to an abnormal heart function (Korostowski et al. PLoS Genet. 2012 8(9):e1002956).

One of the main challenges in cardiac disease research is to identify novel and effective approaches to modulate gene networks or specific intracellular signaling pathways that may prove as effective therapeutic options themselves or have the potential to expand the efficiency of existing therapeutic strategies. It was surprisingly found that specific IncRNAs play a role in the development of cardiac hypertrophy thereby providing novel therapeutic strategies. Thus, the present invention relates in a first aspect to a pharmaceutical composition comprising (i) a compound promoting the expression and/or the activity of one or more long non-coding RNAs (IncRNAs) selected from SEQ ID NOs 12, 8 to 11 and 13; and/or (ii) a compound inhibiting the expression and/or the activity of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28.

In accordance with the present invention, the term "pharmaceutical composition" relates to a composition for administration to a patient, preferably a human patient. The pharmaceutical composition of the invention comprises the compounds recited above. It may, optionally, comprise further molecules capable of altering the characteristics of the compounds of the invention thereby, for example, stabilizing, modulating and/or activating their function. The composition may be in solid, liquid or gaseous form and may be, inter alia, in the form of (a) powder(s), (a) tablet(s), (a) solution(s) or (an) aerosol(s). The pharmaceutical composition of the present invention may, optionally and additionally, comprise a pharmaceutically acceptable carrier or excipient. Examples of suitable pharmaceutical carriers and excipients are well known in the art and include phosphate buffered saline solutions, water, emulsions, such as oil/water emulsions, various types of wetting agents, sterile solutions, organic solvents including DMSO etc. Compositions comprising such carriers or excipients can be formulated by well known conventional methods. These pharmaceutical compositions can be administered to the subject at a suitable dose. The dosage regimen will be determined by the attending physician and clinical factors. As is well known in the medical arts, dosages for any one patient depends upon many factors, including the patient's size, body surface area, age, the particular compound to be administered, sex, time and route of administration, general health, and other drugs being administered concurrently. The therapeutically effective amount for a given situation will readily be determined by routine experimentation and is within the skills and judgement of the ordinary clinician or physician. Generally, the regimen as a regular administration of the pharmaceutical composition should be in the range of 1 pg to 5 g units per day. However, a more preferred dosage might be in the range of 0.01 mg to 100 mg, even more preferably 0.01 mg to 50 mg and most preferably 0.01 mg to 10 mg per day.

Furthermore, if for example said compound is an nucleic acid sequence, such as an siRNA, the total pharmaceutically effective amount of pharmaceutical composition administered will typically be less than about 75 mg per kg of body weight, such as for example less than about 70, 60, 50, 40, 30, 20, 10, 5, 2, 1 , 0.5, 0.1 , 0.05, 0.01 , 0.005, 0.001 , or 0.0005 mg per kg of body weight. More preferably, the amount will be less than 2000 nmol of nucleic acid sequence (e.g., about 4.4 x 1016 copies) per kg of body weight, such as for example less than 1500, 750, 300, 150, 75, 15, 7.5, 1.5, 0.75, 0.15, 0.075, 0.015, 0.0075, 0.0015, 0.00075 or 0.00015 nmol of siRNA agent per kg of body weight.

The length of treatment needed to observe changes and the interval following treatment for responses to occur vary depending on the desired effect. The particular amounts may be determined by conventional tests which are well known to the person skilled in the art.

Pharmaceutical compositions of the invention preferably comprise a pharmaceutically acceptable carrier or excipient. By "pharmaceutically acceptable carrier or excipienf is meant a non-toxic solid, semisolid or liquid filler, diluent, encapsulating material or formulation auxiliary of any type (see also Handbook of Pharmaceutical Excipients 6ed. 2010, Published by the Pharmaceutical Press). The pharmaceutical composition may be administered, for example, orally, parenterally, such as subcutaneously, intravenously, intramuscularly, intraperitoneally, intrathecally, transdermal^, transmucosally, subdurally, locally or topically via iontopheresis, sublingually, by inhalation spray, aerosol or recta I ly and the like in dosage unit formulations optionally comprising conventional pharmaceutically acceptable carriers or excipients.

The term "ncRNA" or "non-coding RNA" as used herein designates a functional RNA molecule that is not translated into a protein. The DNA sequence from which a non-coding RNA is transcribed is often called in the art an RNA gene. The term "IncRNA" or "long non-coding RNA" is commonly used in the art and designates an ncRNA comprising more than 200 nucleotides. SEQ ID NOs 12, 1 to 1 1 , 13, 27 and 28 comprise sequences ranging from 32 to 1598 nucleotides.

The compounds of the invention may be formulated as vesicles, such as liposomes. Liposomes have attracted great interest because of their specificity and the duration of action they offer from the standpoint of drug delivery. Liposomal delivery systems have been used to effectively deliver nucleic acids, such as siRNA in vivo into cells (Zimmermann et al. (2006) Nature, 441 :11 1-114). Liposomes are unilamellar or multilamellar vesicles which have a membrane formed from a lipophilic material and an aqueous interior. The aqueous portion contains the composition to be delivered. Cationic liposomes possess the advantage of being able to fuse to the cell wall. Non-cationic liposomes, although not able to fuse as efficiently with the cell wall, are phagocytosed by macrophages and other cells in vivoA compound inhibiting the expression of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 - as defined herein in item (ii) - is in accordance with the present invention a compound lowering or preventing the transcription of one or more of the genes encoding the IncRNAs selected of SEQ ID NOs 1 to 7, 27 and 28. Such compounds include compounds interfering with the transcriptional machinery and/or its interaction with the promoter of said genes and/or with expression control elements remote from the promoter such as enhancers. The compound inhibiting the expression of an IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28 specifically inhibits the expression of said IncRNA, for example, by specifically interfering with the promoter region controlling the expression of the IncRNA. Preferably, the transcription of an IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 is reduced by at least 50%, more preferred at least 75% such as at least 90% or 95%, even more preferred at least 98% and most preferred by about 100%. A compound inhibiting the activity of an IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 - as defined herein in item (ii) - in accordance with the present invention causes said IncRNA to perform its function with lowered efficiency. The compound inhibiting the activity of an IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28 specifically inhibits the activity of said IncRNA. Preferably, the activity of an IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28 is reduced by at least 50%, more preferred at least 75% such as at least 90% or 95%, even more preferred at least 98%, and most preferably about 100%. Means and methods for determining the reduction of activity of an RNA are established in the art and are described, for example, in Esau et al. (2004), JBC, 279:52361-52365 or Gribbings et al. (2009), Nature Cell Biology 11 , 1 143-1149. Compounds as defined herein in item (ii) may be an antisense molecule, siRNA, shRNA, antibody, ribozyme, aptamer, or small molecule. These and other compounds will be further detailed herein below. The efficiency of an inhibiting compound can be quantified by methods comparing the level of activity in the presence of the inhibitor to that in the absence of the inhibitor. For example, as an activity measure may be used: the change in amount of IncRNA formed. Such method may be effected in high-throughput format in order to test the efficiency of several inhibiting compound simultaneously. High-throughput assays, independently of being biochemical, cellular or other assays, generally may be performed in wells of microtiter plates, wherein each plate may contain 96, 384 or 1536 wells. Handling of the plates, including incubation at temperatures other than ambient temperature, and bringing into contact of test compounds with the assay mixture is preferably affected by one or more computer-controlled robotic systems including pipetting devices. In case large libraries of test compounds are to be screened and/or screening is to be effected within short time, mixtures of, for example 10, 20, 30, 40, 50 or 100 test compounds may be added to each well. In case a well exhibits the expected activity, said mixture of test compounds may be de-convoluted to identify the more test compounds in said mixture giving rise to said activity.

A compound promoting the expression of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 1 1 and 13 - as defined herein in item (i) - may be any compound enhancing or upregulating the transcription of an IncRNA selected from SEQ ID NOs 12, 8 to 11 and 13. Non-limiting examples of such compounds are transcription factors enhancing the transcription of the genes encoding the IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13 or a small molecule enhancing the expression of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13. A transcription factor is a protein binding to specific DNA sequences, thereby controlling the transcription of genetic information from DNA to RNA. A small molecule is a low molecular weight compound which is by definition not a polymer. A compound promoting the activity of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 1 1 and 13 - as defined herein in item (i) - may be any compound which causes that said IncRNA effectively performs its function in a cell. Hence, in the simplest form such a compound may be a recombinantly produced or isolated IncRNAs selected from SEQ ID NOs 12, 8 to 1 1 and 13 or any precursor or fragment thereof. In this embodiment the administration of a recombinantly produced or isolated IncRNA increases the concentration of IncRNA in the subject to be treated. This higher concentration promotes the overall activity of the respective IncRNA in the subject. The fragments have to retain or essentially retain the function of the full-length IncRNA. Such a compound may also be a vector or host being capable of producing such an IncRNAs. Hence, the fragments have to be functional fragments. Also orthologous or homologous sequences of the IncRNA selected from SEQ ID NOs 12, 8 to 1 1 and 13 from different species including precursors or functional fragments thereof may be used. In this regard, preferred homologous sequences of the human IncRNA of SEQ ID NOs 12, 8 to 1 1 and 13 are the respective mouse homologs of SEQ ID NO: 25, 21 to 24 and 26, respectively. The most preferred homologous sequence is SEQ NO: 12. Alternatively, such a compound may be a compound maintaining or even enhancing the activity of an IncRNA selected from SEQ ID NOs 12, 8 to 11 and 13 by either directly or indirectly interacting with the IncRNA. For instance, such a compound may prevent an IncRNA selected from SEQ ID NOs 12, 8 to 1 1 and 13 from degeneration by RNases or may be an interaction partner, such as another IncRNA, which binds to and promotes the activity of an IncRNA selected from SEQ ID NOs 12, 8 to 1 1 and 13. Compounds as defined herein in item (i) will be further detailed herein below. The efficiency of a compound as defined herein in item (i) can also be quantified by methods comparing the level of expression and/or activity of an IncRNA selected from SEQ ID NOs 12, 8 to 1 1 and 13 in the presence of a expression and/or activity promoting compound of the IncRNA, such as a transcription factor, to that in the absence of said compound. For example, as an activity measure the change in amount of IncRNA formed may be used. The method is preferably effected in high-throughput format as further detailed herein above.

The present invention relates in a second aspect to a compound (i) promoting the expression and/or the activity of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13; and/or (ii) inhibiting the expression and/or the activity of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 for use in treating or preventing cardiac hypertrophy.

Compounds as defined herein in items (i) and (ii) have been detailed herein above in connection with the first aspect of the invention. The same compounds can be used in connection with the second aspect of the invention.

Cardiac hypertrophy is defined as an increase in size of the heart without any increase in myocyte number. This results in a thickening of the heart walls. Pathological cardiac hypertrophy occurs in response to haemodynamic overload due to different forms of stress, such as hypertension, valve disease, and myocardial infarction (Ml). Prolonged hypertrophic growth of the heart results in cardiac arrhythmias, heart failure and may lead to sudden death (Frey N, Olson EN. Cardiac hypertrophy: the good, the bad, and the ugly. Annu Rev Physiol. 2003; 65: 45-79). Thus, cardiac hypertrophy is in accordance with the invention unhealthy cardiac hypertrophy (or pathological hypertrophy), such as cardiac hypertrophy in response to stress or disease, e.g., hypertension, heart muscle injury (myocardial infarction), heart failure or neurohormones. Unhealthy cardiac hypertrophy is to be held distinct from healthy cardiac hypertrophy (physiologic hypertrophy or "athlete's heart") which is a normal response of the heart, for example, in response to healthy exercise or pregnancy. Among healthy subjects, rowers or cyclists tend to have the largest hearts, with an average left ventricular wall thickness of 1.3 centimeters, compared to 1.1 centimeters in average adults.

In order to identify IncRNAs playing a role in the development of cardiac hypertrophy the transverse aortic constriction (TAC) mouse model was used (see deAlmeida et la. (2010), J Vis Exp. 2010 Apr, (38)). The TAC mouse model was established in 1991. Since then the model has been extensively used as a valuable tool to mimic human cardiac hypertrophy and to elucidate fundamental signaling processes involved in the cardiac hypertrophic response. The isolated RNA from TAC mice was used for global IncRNA profiling in whole heart as well as cardiomyocyte-specific samples applying the platforms NCode and Arraystar. It was surprisingly found that specific IncRNAs are significantly deregulation in TAC mice as compared to negative control mice. These IncRNAs were selected from the profiling data (see Table 5). The presence of transcripts in mouse heart tissue was verified by PGR and the deregulation was validated by real-time PGR.

As it is evident from the examples herein below, the present invention unexpectedly found that the mouse homologs (i.e. the IncRNAs of SEQ ID NOs 14 to 20) of the human IncRNAs of SEQ ID NOs 1 to 7, 27 and 28 are significantly upregulated in the TAC mouse model as compared to negative control mice. In this respect it is of note that the human sequences SEQ ID NOs 1 , 27 and 28 are all homologs of the mouse sequence of SEQ ID NO: 14. Due to a genomic duplication event distinguishing human and mouse more than one human homolog exists. All three human homolgs were found to be expressed in human heart tissues, and most strikingly were found to be upregulated in hypertrophic hearts from patients with aortic stenosis (see Figure 13 B). The upregulation of these mouse and human homologous IncRNAs is evidently associated with the development of cardiac hypertrophy. Consequently, the mouse IncRNAs of SEQ ID NOs 14 to 20 as well as the homologous human IncRNAs of SEQ ID NOs 1 to 7, 27 and 28 are pro-hypertrophic IncRNAs. It can be expected that the homologous human IncRNAs have the same function as the mouse IncRNAs. It follows that the inhibition of the expression and/or activity of the IncRNAs of SEQ ID NOs 1 to 7, 27 and 28 in humans will be beneficial for the treatment or prevention of cardiac hypertrophy.

As it is furthermore evident from the examples herein below, the present invention reveals that the mouse homologs (i.e. the IncRNAs of SEQ ID NOs 25, 21 to 24 and 26) of the human IncRNAs of SEQ ID NOs 12, 8 to 1 1 and 13 are significantly downregulated in the TAC mouse model as compared to negative control mice. The down regulation of these mouse IncRNAs is evidently associated with the development of cardiac hypertrophy. Consequently, the mouse IncRNAs of SEQ ID NOs 25, 21 to 24 and 26 as well as the homologous human IncRNAs of SEQ ID NOs 12, 8 to 1 1 and 13 are anti-hypertrophic IncRNAs. It follows that the promotion of the expression and/or activity of the IncRNAs of SEQ ID NOs 12, 8 to 1 1 and 13 in humans will be beneficial for the treatment or prevention of cardiac hypertrophy.

The conclusion that the mouse IncRNAs of SEQ ID NOs 14 to 20 are pro-hypertrophic IncRNAs while the mouse IncRNAs of SEQ ID NOs 25, 21 to 24 and 26 are anti-hypertrophic IncRNAs has been further experimentally proven for the pro-hypertrophic RNA IncRNA Gm1 1641 (SEQ ID NO: 14; human homologs SEQ ID NOs 1 , 27 and 28), anti-hypertrophic IncRNA Gm 17499 (SEQ ID NO: 21 , human homolog SEQ ID NO: 8) and anti-hypertrophic IncRNA H19 (SEQ ID NO: 25; human homolog SEQ ID NO:12) by an independent in vitro model. Gm11641 is also referred to herein as Chast (cardiac hypertrophy associated transcript). The hallmark of hypertrophied cardiomyocytes is an increase in cell size, relative to non-hypertrophic cells. In vitro hypertrophic growth and increase in cell size of HL-1 mouse cardiac muscle cells can be induced by phenylephrine (PE) and isoproterenol (ISO). Therefore, in a first experimental setup the cell size of HL-1 mouse cardiac muscle cells after stimulation with PE and ISO was investigated under conditions, wherein (a) the expression of IncRNA Gm11641 is repressed as well as (b) under conditions, wherein the expression of IncRNA Gm11641 is elevated (see Fig. 11 ). In line with the results in the TAC mouse model, it was found that the overexpression of IncRNA Gm11641 leads to an increase of cell size as compared to a negative control, while repression of IncRNA Gm11641 reduced cardiomyocyte size and further attenuated the PE/ISO-induced increase of cell size. Moreover, Gm11641 overexpression increases the muscle mass of the left ventricle and induces cardiomyocyte growth (Fig. 38). These results show the pro-hypertrophic function of IncRNA Gm11641. Corresponding experiments were performed in a second and a third experimental setup with the IncRNAs Gm 17499 and H19, respectively. Also in line with the results in the TAC mouse model, enhanced expression of the IncRNA Gm 17499 prevents HL-1 cell size increase due to pro-hypertrophic stimuli, while silencing of the IncRNA Gm 17499 results in an enlargement of HL-1 cardiomyocytes, indicating an anti-hypertrophic function of this transcript (see Figure 22). These results show the anti-hypertrophic function of the IncRNA Gm17499. Similar results were observed in experiments with the suppression of the IncRNA H19 (see Fig. 28 to 30). Importantly, when expression of H19 was examined in human healthy and hypertrophic heart (due to aortic stenosis) tissue, it was found that H19 is strongly downregulated in hypertrophied hearts (see Fig. 31 ). In addition, in vivo results on H19 knock-down mice indicate that H19 has a beneficial effect on the hypertrophic gene program and is useful in an anti-hypertrophic therapy (see Fig. 34 and 35). In accordance with a preferred embodiment of the second aspect of the invention the cardiac hypertrophy is a ventricular hypertrophy.

Most cases of cardiac hypertrophy affect the heart ventricles. Although left ventricular hypertrophy is more common, cardiac hypertrophy can also occur in the right ventricle or both ventricles. The ventricles are the chambers in the heart responsible for pumping blood either to the lungs (right ventricle) or to the rest of the body (left ventricle). In accordance with a preferred embodiment of the first and second aspect of the invention the compound as defined in (ii) is (a) a nucleic acid sequence which comprises or consists of a nucleotide sequence being complementary to at least 12 continuous nucleotides of a IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28, (b) a nucleic acid sequence which comprises or consists of a nucleotide sequence which is at least 69% identical to the complementary strand of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28, (c) a nucleic acid sequence which comprises or consists of a nucleotide sequence according to (a) or (b), wherein U is replaced by T, (d) an expression vector expressing the nucleic acid sequence as defined in any one of (a) to (c), preferably under the control of a heart-specific promoter, or (e) a host comprising the expression vector of (d).

The term "nucleic acid sequence" or "nucleotide sequence", in accordance with the present invention, includes DNA, such as cDNA or, in a preferred embodiment genomic DNA, and RNA. It is understood that the term "RNA" as used herein comprises all forms of RNA including, in a preferred embodiment, mRNA or miRNA. The term "nucleic acid sequence" is interchangeably used in accordance with the invention with the term "polynucleotide".

The nucleic acid sequences as defined in items (a) to (c) of this preferred embodiment comprise or consist of sequences that comprise or are complementary to nucleotides of an IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28. Hence, these nucleic acid sequences comprise or are antisense nucleic acid sequences. The antisense technology for silencing the expression of a target gene is well-established and widely used in the art to treat various diseases. The molecule according to item (a) of this preferred embodiment of the invention comprises or consists of a sequence which is with increasing preference complementary to at least 13 nucleotides, at least 14 nucleotides, at least 15 nucleotides, at least 16 nucleotides, at least 17 nucleotides, at least 18 nucleotides, at least 19 nucleotides, at least 20 nucleotides, at least 21 nucleotides, at least 22 nucleotides, or all 23 nucleotides of SEQ ID NOs 1 to 7, 27 and 28. These at least 13 nucleotides, at least 14 nucleotides, at least 15 nucleotides, at least 16 nucleotides, at least 17 nucleotides, at least 18 nucleotides, at least 19 nucleotides, at least 20 nucleotides, or at least 21 nucleotides are preferably a contiguous part of SEQ ID NOs 1 to 7, 27 and 28, i.e. the nucleotides are consecutive in the respective SEQ ID NO. The molecule according to item (a) is preferably a "siRNA". The term "siRNA" in accordance with the present invention refers to small interfering RNA, also known as short interfering RNA or silencing RNA. siRNAs are a class of 18 to 30, preferably 20 to 25, most preferred 21 to 23 or 21 nucleotide-long double-stranded RNA molecules that play a variety of roles in biology. Most notably, siRNA is involved in the RNA interference (RNAi) pathway where the siRNA interferes with the expression of a specific gene. In addition to their role in the RNAi pathway, siRNAs also act in RNAi-related pathways, e.g. as an antiviral mechanism or in shaping the chromatin structure of a genome. siRNAs have a well defined structure: a short double-strand of RNA (dsRNA), advantageously with at least one RNA strand having an overhang. Each strand typically has a 5' phosphate group and a 3' hydroxyl (-OH) group. This structure is the result of processing by dicer, an enzyme that converts either long dsRNAs or small hairpin RNAs into siRNAs. siRNAs can also be exogenously (artificially) introduced into cells to bring about the specific knockdown of a gene of interest. Thus, any gene of which the sequence is known can in principle be targeted based on sequence complementarity with an appropriately tailored siRNA. The double-stranded RNA molecule or a metabolic processing product thereof is capable of mediating target-specific nucleic acid modifications, particularly RNA interference and/or DNA methylation. Also preferably at least one RNA strand has a 5'- and/or 3'-overhang. Preferably, one or both ends of the double-strand have a 3'-overhang from 1-5 nucleotides, more preferably from 1-3 nucleotides and most preferably 2 nucleotides. In general, any RNA molecule suitable to act as siRNA is envisioned in the present invention. The most efficient silencing was so far obtained with siRNA duplexes composed of 21-nt sense and 21 -nt antisense strands, paired in a manner to have 2-nt 3'- overhangs. The sequence of the 2-nt 3' overhang makes a small contribution to the specificity of target recognition restricted to the unpaired nucleotide adjacent to the first base pair (Elbashir et al. Nature. 2001 May 24; 411(6836):494-8). 2'-deoxynucleotides in the 3' overhangs are as efficient as ribonucleotides, but are often cheaper to synthesize and probably more nuclease resistant. The siRNA according to the invention comprises an antisense strand which comprises or consists of a sequence which is with increasing preference complementary to at least 13 nucleotides, at least 14 nucleotides, at least 15 nucleotides, at least 16 nucleotides, at least 17 nucleotides, at least 18 nucleotides, at least 19 nucleotides, at least 20 nucleotides, at least 21 nucleotides, at least 22 nucleotides, or all 23 nucleotides of SEQ ID NOs 1 to 7, 27 and 28. These at least 13 nucleotides, at least 14 nucleotides, at least 15 nucleotides, at least 16 nucleotides, at least 17 nucleotides, at least 18 nucleotides, at least 19 nucleotides, at least 20 nucleotides, or at least 21 nucleotides are preferably a contiguous part of SEQ ID NOs 1 to 7, 27 and 28, i.e. the nucleotides are consecutive in the respective SEQ ID NO. The molecule according to item (a) is also preferably a "shRNA". A "shRNA" in accordance with the present invention is a short hairpin RNA, which is a sequence of RNA that makes a (tight) hairpin turn that can also be used to silence gene expression via RNA interference. shRNA preferably utilizes the U6 promoter for its expression. The shRNA hairpin structure is cleaved by the cellular machinery into siRNA, which is then bound to the RNA-induced silencing complex (RISC). This complex binds to and cleaves mRNAs which match the shRNA that is bound to it. The shRNA according to the invention comprises or consists a sequence which is with increasing preference complementary to at least 13 nucleotides, at least 14 nucleotides, at least 15 nucleotides, at least 16 nucleotides, at least 17 nucleotides, at least 18 nucleotides, at least 19 nucleotides, at least 20 nucleotides, at least 21 nucleotides, at least 22 nucleotides, or all 23 nucleotides of SEQ ID NOs 1 to 7, 27 and 28. These at least 13 nucleotides, at least 14 nucleotides, at least 15 nucleotides, at least 16 nucleotides, at least 17 nucleotides, at least 18 nucleotides, at least 19 nucleotides, at least 20 nucleotides, or at least 21 nucleotides are preferably a contiguous part of SEQ ID NOs 1 to 7, 27 and 28, i.e. the nucleotides are consecutive in the respective SEQ ID NO. A molecule according to item (b) of the above preferred embodiment of the invention is capable of interacting with, more specifically hybridizing with the target IncRNA. By formation of the hybrid the function of the IncRNA is reduced or blocked. Standard methods relating to such antisense technology have been described (see, e.g., Melani et al., Cancer Res. (1991 ) 51 :2897-2901 ). The term "antisense molecule" in accordance with the present invention thus relates to a nucleic acid molecule, preferably an RNA molecule that has a base sequence complementary to a given IncRNA, i.e. the "sense" sequence.

A particularly preferred example of the molecule according to item (b) is an Endoribonuclease- prepared siRNA (esiRNA). An esiRNA is a mixture of siRNA oligos resulting from cleavage of a long double-stranded RNA (dsRNA) according to item (b) with an endoribonuclease such as Escherichia coli RNase III or dicer. esiRNAs are an alternative concept to the usage of chemically synthesized siRNA for RNA Interference (RNAi). An esiRNAs is the enzymatic digestion of a long double stranded RNA in vitro. For the generation of esiRNAs a cDNA of an IncRNA template may be amplified by PGR and tagged with two bacteriophage-promotor sequences. RNA polymerase is then used to generate long double stranded RNA that is complentary to the target-gene cDNA. This complentary RNA may be subsequently digested with RNase III from Escherichia coli to generate short overlapping fragments of siRNAs with a length between 18-25 base pairs. This complex mixture of short double stranded RNAs is similar to the mixture generated by Dicer cleavage in vivo and is therefore called endoribonuclease-prepared siRNA or short esiRNA. Hence, esiRNA are a heterogeneous mixture of siRNAs that all target the same mRNA sequence. esiRNAs lead to highly specific and effective gene silencing.

The sequence identity of the molecule according to item (b) to an IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28 is with increasing preference least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99% and 100%. Means and methods for determining sequence identity are known in the art. Preferably, the BLAST (Basic Local Alignment Search Tool) program is used for determining the sequence identity with regard to one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28. Preferred examples of nucleic acid sequences which comprise a nucleotide sequence which is at least 69% identical to the complementary strand of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 are the complementary strand of one or more IncRNAs selected from SEQ ID NOs 14 to 20. Antisense molecules, siRNAs and shRNAs of the present invention are preferably chemically synthesized using appropriately protected ribonucleoside phosphoramidites and a conventional RNA synthesizer. Suppliers of RNA synthesis reagents include Proligo (Hamburg, Germany), Dharmacon Research (Lafayette, CO, USA), Pierce Chemical (part of Perbio Science, Rockford, IL, USA), Glen Research (Sterling, VA, USA), ChemGenes (Ashland, MA, USA), and Cruachem (Glasgow, UK).

The ability of antisense molecules, siRNA, and shRNA to potently, but reversibly, silence IncRNA and genes in vivo makes these molecules particularly well suited for use in the pharmaceutical composition of the invention. Ways of administering siRNA to humans are described in De Fougerolles et al., Current Opinion in Pharmacology, 2008, 8:280-285. Such ways are also suitable for administering other small RNA molecules like shRNA. Accordingly, such pharmaceutical compositions may be administered directly formulated as a saline, via liposome based and polymer-based nanoparticle approaches, as conjugated or complexation pharmaceutical compositions, or via viral delivery systems. Direct administration comprises injection into tissue, intranasal and intratracheal administration. Liposome based and polymer- based nanoparticle approaches comprise the cationic lipid Genzyme Lipid (GL) 67, cationic liposomes, chitosan nanoparticles and cationic cell penetrating peptides (CPPs). Conjugated or complexation pharmaceutical compositions comprise PEI-complexed antisense molecules, siRNA, shRNA or miRNA. Further, viral delivery systems comprise influenza virus envelopes and virosomes. The antisense molecules, siRNAs, shRNAs may comprise modified nucleotides such as locked nucleic acids (LNAs). The ribose moiety of an LNA nucleotide is modified with an extra bridge connecting the 2' oxygen and 4' carbon. The bridge "locks" the ribose in the Z'-endo (North) conformation, which is often found in the A-form duplexes. LNA nucleotides can be mixed with DNA or RNA residues in the oligonucleotide whenever desired. Such oligomers are synthesized chemically and are commercially available. The locked ribose conformation enhances base stacking and backbone pre-organization. This significantly increases the hybridization properties (melting temperature) of oligonucleotides. Particularly preferred example of siRNAs are GapmeRs (LNA™ GapmeRs (Exigon)). GapmeRs are potent antisense oligonucleotides used for highly efficient inhibition of mRNA and IncRNA function. GapmeRs contain a central stretch of DNA monomers flanked by blocks of LNAs. The GapmeRs are preferably 14-16 nucleotides in length and are optionally fully phosphorothioated. The DNA gap activates the RNAse H-mediated degradation of targeted RNAs and is also suitable to target transcripts directly in the nucleus. GapmeRs are used in the examples, e.g., to downregulate the IncRNA Gm1 1641 (SEQ ID NO: 14) in the cardiomyocyte cell line HL-1 (Fig. 9).

Examples of suitable expression vectors which may be used in connection with item (d) of the above-preferred embodiment will detailed herein below.

In accordance with a further preferred embodiment of the first and second aspect of the invention the compound as defined in (ii) is an aptamer, a ribozyme, an antibody, a protein drug, or a small molecule inhibitor. The aptamer, ribozyme, antibody, protein drug, or small molecule inhibitor of this embodiment specifically bind to one or more IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28, thereby inhibiting the activity of one or more IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28.

The term "aptamer" in accordance with the present invention refers to DNA or RNA molecules being either in the natural D-conformation or in the L-conformation ("spiegelmer") that have been selected from random pools based on their ability to bind other molecules. Aptamers have been selected which bind nucleic acid, proteins, small organic compounds, and even entire organisms. A database of aptamers is maintained at http://aptamer.icmb.utexas.edu/. More specifically, aptamers can be classified as DNA or RNA aptamers or peptide aptamers. Whereas the former consist of (usually short) strands of oligonucleotides, the latter consist of a short variable peptide domain, attached at both ends to a protein scaffold. Nucleic acid aptamers are nucleic acid species that have been engineered through repeated rounds of in vitro selection or equivalently, SELEX (systematic evolution of ligands by exponential enrichment) to bind to various molecular targets such as small molecules, proteins, nucleic acids, and even cells, tissues and organisms. The molecular target envisaged by the present invention is a nucleic acid, namely an IncRNA selected from 1 to 7, 27 and 28. Hence, aptamers can be produced against the target molecule of the invention. Peptide aptamers are peptides that are designed to interfere with other protein interactions inside cells. They consist of a variable peptide loop attached at both ends to a protein scaffold. This double structural constraint greatly increases the binding affinity of the peptide aptamer to levels comparable to an antibody's (nanomolar range). The variable loop length is typically comprised of 10 to 20 amino acids, and the scaffold may be any protein which has good solubility properties. Currently, the bacterial protein Thioredoxin-A is the most used scaffold protein, the variable loop being inserted within the reducing active site, which is a -Cys-Gly-Pro-Cys- loop in the wild protein, the two cysteins lateral chains being able to form a disulfide bridge. Peptide aptamer selection can be made using different systems, but the most used is currently the yeast two-hybrid system.

Aptamers offer the utility for biotechnological and therapeutic applications as they offer molecular recognition properties that rival those of the commonly used biomolecules, in particular antibodies. In addition to their discriminate recognition, aptamers offer advantages over antibodies as they can be engineered completely in a test tube, are readily produced by chemical synthesis, possess desirable storage properties, and elicit little or no immunogenicity in therapeutic applications. Non-modified aptamers are cleared rapidly from the bloodstream, with a half-life of minutes to hours, mainly due to nuclease degradation and clearance from the body by the kidneys, a result of the aptamer's inherently low molecular weight. The rapid clearance of aptamers can be an advantage in applications such as in vivo diagnostic imaging. Several modifications, such as 2'-fluorine-substituted pyrimidines, polyethylene glycol (PEG) linkage, etc. are available to scientists with which the half-life of aptamers easily can be increased to the day or even week time scale. The term "ribozymes" refers to RNA molecules that act as enzymes in the absence of proteins. These RNA molecules act catalytic or autocatalytic and are capable of cleaving e.g. other RNAs at specific target sites but they have also been found to catalyze the aminotransferase activity of the ribosome. Selection of appropriate target sites and corresponding ribozymes can be done as described for example in Zaher and Unrau (2007), RNA 13 (7): 1017-1026. Examples of well-characterized small self-cleaving RNAs are the hammerhead, hairpin, hepatitis delta virus, and in vitro-selected lead-dependent ribozymes. The organization of these small catalysts is in contrast to that of larger ribozymes, such as the group I intron.

The principle of catalytic self-cleavage has become well established in the last 10 years. The hammerhead ribozymes are characterized best among the RNA molecules with ribozyme activity. Since it was shown that hammerhead structures can be integrated into heterologous RNA sequences and that ribozyme activity can thereby be transferred to these molecules, it appears that catalytic sequences for almost any target sequence can be created, provided the target sequence contains a potential matching cleavage site.

The basic principle of constructing hammerhead ribozymes is as follows: An interesting region of the RNA, which contains the GUC (or CUC) triplet, is selected. Two oligonucleotide strands, each with 6 to 8 nucleotides, are taken and the catalytic hammerhead sequence is inserted between them. Molecules of this type were synthesized for numerous target sequences. They showed catalytic activity in vitro and in some cases also in vivo. The best results are usually obtained with short ribozymes and target sequences. Since the target sequence is a short RNA sequence, namely an IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28. IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 are bona fide targets sequences for the generation of ribozymes being capable to specifically cleave an IncRNA selected from SEQ ID NOS 1 to 7, 27 and 28.

Also the aptamers and ribozymes may comprise modified nucleotides, such as locked nucleic acids (LNAs).

The term "antibody" as used in accordance with the present invention comprises, for example, polyclonal or monoclonal antibodies. Furthermore, also derivatives or fragments thereof, which still retain the binding specificity, are comprised in the term "antibody". Antibody fragments or derivatives comprise, inter alia, Fab or Fab' fragments, Fd, F(ab')₂, Fv or scFv fragments, single domain V_H or V-like domains, such as VhH or V-NAR-domains, as well as multimeric formats such as minibodies, diabodies, tri bodies, tetrabodies or chemically conjugated Fab'- multimers (see, for example, Altshuler et al., 2010., Holliger and Hudson, 2005). The term "antibody" also includes embodiments such as chimeric (human constant domain, non-human variable domain), single chain and humanized (human antibody with the exception of non- human CDRs) antibodies. Various techniques for the production of antibodies and fragments thereof are well known in the art and described, e.g. in Altshuler et al., 2010. Thus, polyclonal antibodies can be obtained from the blood of an animal following immunisation with an antigen in mixture with additives and adjuvans and monoclonal antibodies can be produced by any technique which provides antibodies produced by continuous cell line cultures. Examples for such techniques are described, e.g. Harlow and Lane (1988) and (1999) and include the hybridoma technique originally described by Kohler and Milstein, 1975, the trioma technique, the human B-cell hybridoma technique (see e.g. Kozbor, 1983; Li et al., 2006) and the EBV-hybridoma technique to produce human monoclonal antibodies (Cole et al., 1985). Furthermore, recombinant antibodies may be obtained from monoclonal antibodies or can be prepared de novo using various display methods such as phage, ribosomal, mRNA, or cell display. A suitable system for the expression of the recombinant (humanized) antibodies or fragments thereof may be selected from, for example, bacteria, yeast, insects, mammalian cell lines or transgenic animals or plants (see, e.g., US patent 6,080,560; Holliger and Hudson, 2005). Further, techniques described for the production of single chain antibodies (see, inter alia, US Patent 4,946,778) can be adapted to produce single chain antibodies specific for the target of this invention. Surface plasmon resonance as employed in the BIAcore system can be used to increase the efficiency of phage antibodies.

The term "protein drug" designates designer drugs that are derivatives of human proteins. These proteins are used as scaffold to create a protein drug by well-established screening procedures (see Tomlinson et al (2004), NATURE BIOTECHNOLOGY, 22(5): 521 -522). Non- limiting examples of human proteins which serve as a scaffold for designing protein drugs are transferrin, C-type lectins, trinectins, domain antibodies, kunitz domains, lipocalins and the Fyn SH3 domain. A small molecule inhibitor is a low molecular weight organic compound which is by definition not a polymer. The small molecule of the invention is preferably a molecule that binds with high affinity to an IncRNA of SEQ ID NOs 1 to 7, 27 and 28 and in addition inhibits the activity of an IncRNA of SEQ ID NOs 1 to 7, 27 and 28. The upper molecular weight limit for a small molecule is preferably 1500Da, more preferably 1000Da and most preferably 800Da which allows for the possibility to rapidly diffuse across cell membranes so that they can reach intracellular sites of action. Libraries of small organic molecules and high-throughput techniques for screening such libraries with a specific target molecule, in the present case an IncRNA selected from SEQ ID NOs 1 to 7, 27 and 28, are established in the art. Antisense molecule, siRNA, shRNA, antibody, enzyme, ribozyme, aptamer, protein drug, or small molecule inhibitor may be fused to a lipid, such as a cholesterol. Means and methods to introduce lipid modifications and in particular a cholesterol modification to a nucleic acid molecule are described in Krutzfeldt et al. 2005 (Nature 438, 685-689). For example, a cholesterol may be linked through a hydroxylprolinol linkage to a nucleic acid molecule. Such modifications increase the efficiency of the uptake of a nucleic acid molecule and in particular of small RNAs into the cell.

In accordance with another preferred embodiment of the first and second aspect of the invention the compound as defined in (i) is (a) a nucleic acid sequence which comprises or consists of the nucleic acid sequence of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13 or an nucleic acid sequence which is at least 69% identical thereto, (b) an expression vector expressing the nucleic acid sequence as defined in (a), preferably under the control of a heart-specific promoter, or (c) a host comprising the expression vector of (b).

The nucleic acid sequence according to item (a) of this preferred embodiment may be a recombinantly produced or isolated IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13, any precursor thereof or any fragment thereof as long as a sequence identity of at least 69% over the entire length of an IncRNA selected from SEQ ID NOs 12, and 8 to 11 and 13 is maintained. Also orthologous or homologous sequences of the IncRNA selected from SEQ ID NOs 12, 8 to 11 and 13 from different species including precursor or a functional fragment thereof may be used. Preferably the respective mouse homologs of SEQ ID NO: 21 to 26 are used. The fragments have to retain or essentially retain the function of the full-length IncRNA. Hence, the fragments have to be functional fragments. Particularly preferred examples of sequences comprising a nucleic acid sequence which is at least 69% identical to IncRNAs of SEQ ID NOs 8 to 13 are the mouse homologous IncRNAs of SEQ ID NOs 21 to 26, respectively. The most preferred mouse homologous IncRNA is SEQ ID NO: 25.

The sequence identity of the nucleic acid sequence according to item (a) to an IncRNA selected from SEQ ID NOs 12, 8 to 11 and 13 is with increasing preference at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99% and 100%. Means and methods for determining sequence identity are known in the art. Preferably, the BLAST (Basic Local Alignment Search Tool) program is used for determining the sequence identity with regard to one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13. In accordance with items (b) and (c) of the above preferred embodiment such a compound may also be an expression vector or host being capable of producing an nucleic acid sequence as defined in item (a). An expression vector may be a plasmid that is used to introduce a specific transcript into a target cell. Once the expression vector is inside the cell, the protein that is encoded by the gene is produced by the cellular-transcription and translation machinery ribosomal complexes. The plasmid is in general engineered to contain regulatory sequences that act as enhancer and/or promoter regions and lead to efficient transcription of the transcript. In accordance with the present invention the expression vector preferably contains a heart-specific promoter. Heart-specific promoters are known in the art, for example, from Boecker at al. (2004), Mol Imagin.; 3(2):69-75. This ensures that the nucleic acid sequence is only expressed in the heart and may avoid potential unwanted side effects by expression in other organs. Non-limiting examples of expression vectors include prokaryotic plasmid vectors, such as the pUC-series, pBluescript (Stratagene), the pET-series of expression vectors (Novagen) or pCRTOPO (Invitrogen) and vectors compatible with an expression in mammalian cells like pREP (Invitrogen), pcDNA3 (Invitrogen), pCEP4 (Invitrogen), pMCI neo (Stratagene), pXT1 (Stratagene), pSG5 (Stratagene), EBO-pSV2neo, pBPV-1 , pdBPVMMTneo, pRSVgpt, pRSVneo, pSV2-dhfr, plZD35, pLXIN, pSIR (Clontech), pIRES-EGFP (Clontech), pEAK-10 (Edge Biosystems) pTriEx-Hygro (Novagen) and pCINeo (Promega). Examples for plasmid vectors suitable for Pichia pastoris comprise e.g. the plasmids pA0815, pPIC9K and pPIC3.5K (all Intvitrogen). For the formulation of a pharmaceutical composition a suitable vector is selected in accordance with good manufacturing practice. Such vectors are known in the art, for example, from Ausubel et al, Hum Gene Ther. 201 1 Apr; 22(4):489-97 or Allay et al., Hum Gene Ther. May 2011 ; 22(5): 595-604.

A typical mammalian expression vector contains the promoter element, which mediates the initiation of transcription of mRNA, the protein coding sequence, and signals required for the termination of transcription and polyadenylation of the transcript. Moreover, elements such as origin of replication, drug resistance gene, regulators (as part of an inducible promoter) may also be included. The lac promoter is a typical inducible promoter, useful for prokaryotic cells, which can be induced using the lactose analogue isopropylthiol-b-D-galactoside. ("IPTG"). For recombinant expression and secretion, the polynucleotide of interest may be ligated between e.g. the PelB leader signal, which directs the recombinant protein in the periplasm and the gene III in a phagemid called pHEN4 (described in Ghahroudi et al, 1997, FEBS Letters 414:521-526). Additional elements might include enhancers, Kozak sequences and intervening sequences flanked by donor and acceptor sites for RNA splicing. Highly efficient transcription can be achieved with the early and late promoters from SV40, the long terminal repeats (LTRs) from retroviruses, e.g., RSV, HTLVI, HIVI, and the early promoter of the cytomegalovirus (CMV). However, cellular elements can also be used (e.g., the human actin promoter). Suitable expression vectors for use in practicing the present invention include, for example, vectors such as pSVL and pMSG (Pharmacia, Uppsala, Sweden), pRSVcat (ATCC 37152), pSV2dhfr (ATCC 37146) and pBC12MI (ATCC 67109). Alternatively, the recombinant (poly)peptide can be expressed in stable cell lines that contain the gene construct integrated into a chromosome. The co-transfection with a selectable marker such as dhfr, gpt, neomycin, hygromycin allows the identification and isolation of the transfected cells. The transfected nucleic acid can also be amplified to express large amounts of the encoded (poly)peptide. The DHFR (dihydrofolate reductase) marker is useful to develop cell lines that carry several hundred or even several thousand copies of the gene of interest. Another useful selection marker is the enzyme glutamine synthase (GS) (Murphy et al.1991 , Biochem J. 227:277-279; Bebbington et al. 1992, Bio/Technology 70:169-175). Using these markers, the mammalian cells are grown in selective medium and the cells with the highest resistance are selected. As indicated above, the expression vectors will preferably include at least one selectable marker. Such markers include dihydrofolate reductase, G418 or neomycin resistance for eukaryotic cell culture and tetracycline, kanamycin or ampicillin resistance genes for culturing in E. coli and other bacteria. For vector modification techniques, see Sambrook and Russel (2001 ), Molecular Cloning: A Laboratory Manual, 3 Vol.. Generally, vectors can contain one or more origins of replication (ori) and inheritance systems for cloning or expression, one or more markers for selection in the host, e.g., antibiotic resistance, and one or more expression cassettes. Suitable origins of replication (ori) include, for example, the Col E , the SV40 viral and the M 3 origins of replication.

The coding sequences inserted in the vector can e.g. be synthesized by standard methods, or isolated from natural sources. Ligation of the coding sequences to transcriptional regulatory elements and/or to other amino acid encoding sequences can be carried out using established methods. Transcriptional regulatory elements (parts of an expression cassette) ensuring expression in prokaryotes or eukaryotic cells are well known to those skilled in the art. These elements comprise regulatory sequences ensuring the initiation of the transcription (e.g., translation initiation codon, promoters, enhancers, and/or insulators), internal ribosomal entry sites (IRES) (Owens, Proc. Natl. Acad. Sci. USA 98 (2001 ), 1471-1476) and optionally poly-A signals ensuring termination of transcription and stabilization of the transcript. Additional regulatory elements may include transcriptional as well as translational enhancers, and/or naturally-associated or heterologous promoter regions. Preferably, the nucleotide sequence as defined in item (a) of the above preferred embodiment of the invention is operatively linked to such expression control sequences allowing expression in prokaryotic or eukaryotic cells.

The host may be a prokaryotic or eukaryotic cell. A suitable eukaryotic host may be a mammalian cell, an amphibian cell, a fish cell, an insect cell, a fungal cell or a plant cell. Representative examples of bacterial cells are E. coli, Streptomyces and Salmonella typhimurium cells; of fungal cells are yeast cells; and of insect cells are Drosophila S2 and Spodoptera Sf9 cells. It is preferred that the cell is a mammalian cell such as a human cell. Mammalian host cells that could be used include, human Hela, 293, H9 and Jurkat cells, mouse NIH3T3 and C127 cells, Cos 1 , Cos 7 and CV1 , quail QC1-3 cells, mouse L cells and Chinese hamster ovary (CHO) cells. The cell may be a part of a cell line, preferably a human cell line. Appropriate culture mediums and conditions for the above-described host cells are known in the art. The host is preferably a host cell and more preferably an isolated host cell. The host is also preferably a non-human host.

In accordance with another preferred embodiment of the first and second aspect of the invention the compound as defined in (i) is (a) a transcription factor promoting the expression of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13, and/or (b) a small molecule enhancing the expression of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 1 1 and 13.

The term "transcription factor" as used herein defines a protein or peptide that binds to specific DNA sequences, thereby controlling the transcription of the genes encoding of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 1 1 and 13. The efficiency of a transcription factor in activating the expression of an IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13 can be quantified by methods comparing the level of the IncRNA in the presence of the transcription factor to that in the absence of the transcription factor. For example, as an activity measure the change in amount of IncRNA formed may be used. Such a method may be effected in high-throughput format in order to test the efficiency of several inhibiting compound simultaneously. High-throughput formats have been further detailed herein above.

The small molecule enhancing the expression of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 1 1 and 13 is a low molecular weight organic compound which is by definition not a polymer. The small molecule of the invention is preferably a molecule that binds with high affinity to an IncRNA of SEQ ID NOs 12, 8 to 11 and 13 and in addition enhances the activity of an IncRNA of SEQ ID NOs 11 , 8 to 11 and 13. The upper molecular weight limit for a small molecule is preferably 1500Da, more preferably 1000Da and most preferably 800Da which allows for the possibility to rapidly diffuse across cell membranes so that they can reach intracellular sites of action. Libraries of small organic molecules and high-throughput techniques for screening such libraries with a specific target molecule, in the present case an IncRNA selected from SEQ ID NOs 12, 8 to 11 and 13, are established in the art.

In a third aspect the present invention relates to a method for diagnosing cardiac hypertrophy in a patient, comprising (a) detecting the expression level of one or more IncRNAs selected from SEQ ID NOs 12, 1 to 11 , 13, 27 and 28 in a sample obtained from said patient, and (b) comparing said expression level of the one or more IncRNAs with the expression level of these one or more IncRNAs in a sample obtained from healthy subjects, wherein a greater than 2-fold downregulation of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28; and/or a greater than 2-fold upregulation of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13 is indicative for a cardiac hypertrophy in the patient.

The method according to the third aspect of the invention may also encompass detecting and comparing the expression level of one or more IncRNAs being with increased preference at least 90%, at least 92%, at least 94%, at least 96%, at least 98%, at least 99%, and at least 99.5% identical to any one of SEQ ID NOs 12, 1 to 11 , 13, 27 and 28. Means and methods for determining sequence identity are known in the art. Preferably, the BLAST (Basic Local Alignment Search Tool) program is used for determining the sequence identity with regard to one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28 and/or 12, 8 to 11 and 13. The method according to the third aspect of the invention may furthermore encompass detecting and comparing the expression level of one or more IncRNAs differing with increasing preference by no more than 10, such as 5, 4, 3, 2 or 1 nucleotide(s) from any one of SEQ ID NOs 1 to 7, 27 and 28 and/or 12, 8 to 11 and 13. The nucleotide differences may be the addition, deletion and/or substitution of nucleotide(s). The sequences the expression of which is compared, while being homologous, may also differ from each other with increasing preference by no more than 10, such as 5, 4, 3, 2 or 1 nucleotide(s).

The term "sample" designates a tissue sample or a body fluid sample. The body fluid sample is preferably selected from blood, serum, plasma, urine, salvia, amniotic fluid, cerebrospinal fluid and lymph. The tissue sample is preferably an organ sample, such as a heart, liver or kidney sample. As far as the method is applied to a body fluid sample it is to be understood that the expression level of an IncRNA corresponds to the concentration of the IncRNA, because IncRNAs are not directly expressed in the body fluid but secreted from the cells, said cells expressing the IncRNAs, into the body fluids. The "patient" or "subject" referred to herein is human.

The term "detecting the expression level of IncRNA" means determining the amount or yield of the IncRNA. The IncRNAs are initially expressed within a cell. It was found in accordance with the present invention that the IncRNAs of SEQ ID NOs 1 to 7, 27 and 28 and/or 12, 8 to 1 1 and 13 can be detected in the sample of a patient, in particular a heart tissue sample. An IncRNA being "expressed in a sample" is therefore a IncRNA whose expression level can be detected in the sample by means and methods being further detailed herein below.. An ncRNA is upregulated in a test sample if the amount or yield of the ncRNA is significantly greater as compared to the amount or yield of the corresponding ncRNA in a control sample. Likewise, an ncRNA is downregulated in a test sample if the amount or yield of the ncRNA is significantly less as compared to the amount or yield of the corresponding ncRNA in a control sample. In this context the term "corresponding ncRNA" means, for example, that the expression level of the IncRNA of SEQ ID NO: 1 in the test sample is compared to the expression level of the IncRNA of SEQ ID NO: 1 in the control sample, or likewise that the expression level of the IncRNA of SEQ ID NO: 2 in the test sample is compared to the expression level of the IncRNA of SEQ ID NO: 2 in the control sample. This applies mutatis mutandis for scenarios where the expression of more than one IncRNA selected from SEQ ID NOs 12, 1 to 11 , 13, 27 and 28 is determined. For instance, if the expression level of all IncRNAs of SEQ ID NOs 12, 1 to 11 , 13, 27 and 28 is determined in the test sample it is compared to the expression level of all IncRNAs of SEQ ID NOs 12, 1 to 11 , 13, 27 and 28 in the control sample.

The expression level in the samples can be quantified by any suitable means and methods available from the art. In general relative and absolute quantification means and methods can be used. In absolute quantification no known standards or controls are needed. The expression level can be directly quantified. As well-known in the art, absolute quantification may rely on a predetermined standard curve. In relative quantification the expression level is quantified relative to a reference (such as known control expressions levels). Also in the absence of controls, one can relatively quantify the expression level when comparing e.g. fluorescence intensities. Methods to assess RNA concentration may, for example, comprise measuring the fluorescence intensity of dyes that bind to nucleic acids and selectively fluoresce when bound. Such methods comprise a reverse transcription reaction and the production of cDNA, wherein the amount of the cDNA is determined thereby indirectly determining the amount of the RNA. The fluorescent-based method is particularly useful for cases where the RNA concentration is too low to accurately assess some with spectrophotometry and/or in cases where contaminants absorbing at 260nm make accurate quantification by spectrophotometry difficult or impossible.

When comparing the expression level of the one or more IncRNAs between different samples reliability of the comparison is preferably improved by including an invariant endogenous control (expression of a reference gene) to correct for potential sample to sample variations. Such normalization with respect to an invariant endogenous control is routinely performed in the art. For example, means and methods for expression level normalization, e.g. in real-time RT-PCR (see, for example, Bustin, Journal of Molecular Endocrinology, (2002) 29, 23-39) or micro-array expression analysis (see, for example, Calza and Balwitan, Methods Mol Biol. 2010;673:37-52) are well-established. Also methods for normalization of the expression levels of small RNA sequences are established (see, for example, Mestdagh et al. (2009) Genome Biol.; 0(6):R64). In case RT-PCR or a micro-array is used to determine the expression levels in accordance with the present invention, the expression levels are preferably normalized to a spiked-in RNA (see, for example, McCormick et al. (2011 ), Silence, 2:2). Known amounts of a spiked-in RNA are mixed with the sample during preparation. More preferably the RNA is externally spiked-in to plasma and/or serum before the RNA isolation process is carried out, in which case the samples are plasma and/or serum. The spiked-in RNA technology is well- known and commercial kits are available from a number of manufacturers. The spiked-in RNA is preferably a spiked-in C. elegans RNA.

As evident from the examples herein below, the deregulation of the levels of one or more mouse IncRNAs selected from 14 to 20 and 15 to 26 are indicative for cardiac hypertrophy as evidenced in the TAC mouse model. Thus, determining the expression levels of one or more of the respective human homologous IncRNAs selected from 12, 1 to 11 , 13, 27 and 28 can be expected to be of prognostic value for diagnosing a cardiac hypertrophy in a patient. The IncRNAs selected from 12, 1 to 1 1 , 13, 27 and 28 may be combined with further diagnostic markers for cardiac hypertrophy in order to enhance the confidentially of the diagnostic method. High-expression level of the IncRNAs selected from 1 to 7, 27 and 28 and low expression level of the IncRNAs selected from 12, 8 to 11 and 13 is indicative for cardiac hypertrophy. In the examples herein below the primer sequences of SEQ ID NOs 29 to 62 were employed in order to detect the expression level of IncRNA, wherein the uneven numbers are forward primers and the even number are reverse primers. Consecutive numbers, such as SEQ ID NOs 29 and 30, SEQ ID NOs 31 and 32, SEQ ID NOs 33 and 34 etc. are a primer pair. The primer pair of SEQ ID NOs 29/30 is for the detection of the expression level of the mouse IncRNA H19 (SEQ ID NO: 25) while the primer pair of SEQ ID NOs 31/32 is for the detection of the expression level of the human IncRNA H19 (SEQ ID NO: 12). The primer pair of SEQ ID NOs 39/40 is for the detection of the expression level of the mouse IncRNA Gm11641 (SEQ ID NO: 14), while the expression levels of the three human homologous IncRNAs SEQ ID NOs 1 , 27 and 28 can be detected by the primer pairs of SEQ ID NOs 33/34, SEQ ID NOs 35/36 and SEQ ID NOs 37/38, respectively.

One or more of these primer pairs are preferably used in the diagnostic method according to the third aspect of the invention. One or more of these primer pairs are likewise preferably incorporated into the kit of the invention being described herein below.

The greater than 2-fold downregulation is with increasing preference greater than 3-fold downregulation, greater than 4-fold downregulation, greater than 5-fold downregulation, greater than 6-fold downregulation, greater than 7-fold downregulation and greater than 8-fold downregulation. Likewise the greater than 2-fold upregulation is with increasing preference greater than 3-fold upregulation, greater than 4-fold upregulation, greater than 5-fold upregulation, greater than 6-fold upregulation, greater than 7-fold upregulation and greater than 8-fold upregulation. The higher thresholds for the up- and downregulation may increase the reliability of the method of the third aspect of the invention. In accordance with a preferred embodiment of the third aspect of the invention the sample is a blood sample or blood-derived sample.

The blood -derived sample is preferably plasma or serum. In accordance with another preferred embodiment of the third aspect of the invention the sample is a heart tissue sample.

The heart tissue sample comprises preferably muscle cells of the heart wall and most preferably muscle cells of the ventricular wall. In accordance with a further preferred embodiment of the third aspect of the invention the detection of the expression level of the one or more IncRNAs comprises (a) quantitative PCR, preferably quantitative real time PCR, or (b) a template/RNA amplification method followed by determining the expression level of the one or more IncRNAs using a fluorescence- or luminescence-based quantification method.

In quantitative PCR (qPCR), the amount of amplified product is linked to fluorescence intensity using a fluorescent reporter molecule. The point at which the fluorescent signal is measured in order to calculate the initial template quantity can either be at the end of the reaction (endpoint semi-quantitative PCR) or while the amplification is still progressing (real-time qPCR).

In endpoint semi-quantitative PCR, fluorescence data are collected after the amplification reaction has been completed, usually after 30-40 cycles, and this final fluorescence is used to back-calculate the amount of template present prior to PCR.

The more sensitive and reproducible method of real-time qPCR measures the fluorescence at each cycle as the amplification progresses. This allows quantification of the template to be based on the fluorescence signal during the exponential phase of amplification, before limiting reagents, accumulation of inhibitors, or inactivation of the polymerase have started to have an effect on the efficiency of amplification. Fluorescence readings at these earlier cycles of the reaction will measure the amplified template quantity where the reaction is much more reproducible from sample to sample than at the endpoint.

A non-limiting example of a template/RNA amplification method followed by determining the expression level of the one or more IncRNAs using a fluorescence- or luminescence-based quantification method is a method combining transcription mediated amplification (TMA) and a hybridization protection assay (HPA). In more detail, such a method may comprise hybridizing one or more oligonucleotides ("capture oligonucleotides") that are complementary to any of SEQ ID NOs 12, 1 to 11 , 13, 27 and 28. In case two or more of SEQ ID NOs 12, 1 to 1 1 , 13, 27 and 28 are targeted, a separate capture oligonucleotides is used for each sequence selected from 12, 1 to 1 1 , 13, 27 and 28. The hybridized target sequences are then captured onto magnetic microparticles that are separated from the sample in a magnetic field. Wash steps may be utilized to remove extraneous components. Target amplification typically occurs via TMA, which is a transcription-based nucleic acid amplification method that utilizes two enzymes, Moloney murine leukemia virus (MMLV) reverse transcriptase and T7 RNA polymerase. A unique set of primers is used for each target sequence selected from 12, 1 to 11 , 13, 27 and 28. The reverse transcriptase is used to generate a DNA copy (containing a promoter sequence for T7 RNA polymerase) of the target sequence. T7 RNA polymerase produces multiple copies of RNA amplicon from the DNA copy. Detection of IncRNA expression level is achieved by HPA using single-stranded, chemiluminescent-labeled nucleic acid probes that are complementary to the one or more amplicon. Preferably, distinguishably labelled probes are used for each target amplicon. The labeled nucleic acid probes hybridize specifically to the amplicon. A "selection reagent" then differentiates between hybridized and unhybridized probes by inactivating the label on unhybridized probes. During the detection step, the chemiluminescent signal produced by the hybridized probe is measured in a luminometer and is reported as "Relative Light Units" (RLU), thereby quantifying the IncRNA expression level.

In accordance with a still further preferred embodiment of the third aspect of the invention the method comprises prior to the detection of the expression level of the long non-coding RNA a pre-amplifi cation step of the RNA within the test patient's sample and/or the control patient's sample.

Performing a pre-amplification step is of particular advantage in case only a low amount of (test and/or control) sample is available. The pre-amplifi cation step allows increasing the amount of RNA within the sample before proceeding to the analysis of the expression level. Means and methods for the pre-amplification of RNA are well known in the art (see, e.g., Vermeulen et al (2009) BMC Res Notes., 2:235). In case both the RNA in the test and control sample is pre-amplified preferably the same method for the pre-amplification step is used such that the relative amount of RNA of the test sample as compared to the control sample is maintained. In case only the RNA of the test or control sample is pre-amplified or the two RNA samples are pre-amplified by different methods, the expression level data may have to be normalized for pre-amplification step; see, e.g. Mestdagh et al. (2009), Genome Biology 2009, 10:R64. In a fourth aspect the present invention relates to a kit for diagnosing cardiac hypertrophy in a patient, said kit comprising means for the detection of the expression level of one or more IncRNAs selected from SEQ ID NOs 12, 1 to 11 , 13, 27 and 28, and instructions how to use the kit. The instructions how to use the kit preferably inform inter alia that high-expression level of the IncRNAs selected from 1 to 7, 27 and 28 and low expression level of the IncRNAs selected from 12, 8 to 11 and 13 is indicative for cardiac hypertrophy.

The means for the detection of the expression level of one or more IncRNAs selected from SEQ ID NOs 12, 1 to 11 , 13, 27 and 28 are preferably the means required for (i) a quantitative PCR, preferably quantitative real time PGR, or (ii) a template/RNA amplification method followed by determining the expression level of the one or more IncRNAs using a fluorescence- or luminescence-based quantification method. These means have been further detailed herein above in connection with the third aspect of the invention, and may be comprised in the kit. Hence, the means preferably comprise oligonucleotides, such as fluorescent hybridization probes or primers, which specifically hybridize to one or more IncRNAs selected from SEQ ID NOs 12, 1 to 11 , 13, 27 and 28. Additional ingredients of the kits may be florescent or luminescent dyes, preferably coupled to said oligonucleotides. Also, additional ingredients of the kits may be enzymes, such as a reverse transcriptase and/or a polymerase.

In accordance with the kit of the invention the means for the detection of the expression level of one or more IncRNAs selected from SEQ ID NOs 12, 1 to 11 , 13, 27 and 28 preferably comprise means for the detection of the IncRNA of SEQ ID NO: 12. The various components of the kit may be packaged in one or more containers such as one or more vials. The vials may, in addition to the components, comprise preservatives or buffers for storage.

In accordance with a preferred embodiment of the fourth aspect of the invention, the means are primer pairs used for the specific detection of the expression level of one or more IncRNAs selected from SEQ ID NOs 12, 1 to 11 , 13, 27 and 28.

In accordance with a preferred embodiment of all four aspects of the invention the one or more IncRNAs are at least 3 IncRNAs, and preferably at least 5 IncRNAs.

Employing at least 3 IncRNAs, preferably at least 5 IncRNAs, more preferably at least 10 IncRNAs, even more preferably at least 20 IncRNAs and most preferably all IncRNAs of SEQ ID NOs 12, 1 to 11 , 13, 27 and 28 will additionally increase the effectiveness of the pharmaceutical compositions, medical uses, methods and kits of the invention. Employing these numbers of IncRNAs may balance potential differences associated with particular compounds, probes or methods used in connection with the methods and kits of the invention. In the pharmaceutical compositions and medical uses of the invention these numbers of IncRNAs may increase the beneficial effect for the subject to be treated.

In accordance with a preferred embodiment of all four aspects of the invention the one or more IncRNAs is or comprises the IncRNA of SEQ ID NO: 12.

SEQ ID NO: 12 is the human IncRNA H19. The homologous mouse IncRNA H19 is represented by SEQ ID NO: 25. The anti-hypertrophic nature of IncRNA H19 is demonstrated in example 3 herein below. The gene of the H19 IncRNA is found in humans and other mammals. The regulation of the H19 gene has been well described as a paradigm of genomic imprinting and further has been implicated in human genetic disorders and cancer. After birth, H19 is predominantly expressed in muscle tissue where it promotes differentiation and regeneration. The IncRNAs biological function in the heart remained unclear. To the best knowledge of the inventors a role or function of H19 in cardiac hypertrophy is unknown from the prior art and surprisingly found in connection with the present invention. H19 is evolutionary conserved across various mammalian species including mouse and human (see Fig. 32 and 33).

In accordance with a further preferred embodiment of all four aspects of the invention the one or more IncRNAs is or comprises the IncRNA of SEQ ID NO: 1 , 27 or 28, wherein SEQ ID NO: 1 is most preferred.

As discussed herein above SEQ ID NOs 1 , 27 and 28 are the human homologous IncRNAs of the mouse IncRNA Gm1 1641 (SEQ ID NO: 14), noting that a genomic duplication event distinguishes human and mouse and gives rise to more than one homologous human IncRNA of the respective mouse IncRNA (see Figure 13 A and B). The pro-hypertrophic nature of IncRNA Gm1 1641 is demonstrated in the example herein below by two independent cardiac hypertrophy test systems, being the TAC mouse model and phenylephrine (PE) and isoproterenol (ISO) treated HL-1 mouse cardiac muscle cells.

In accordance with a still further preferred embodiment of all four aspects of the invention the one or more IncRNAs is or comprises the IncRNA of SEQ ID NO: 8.

As discussed herein above SEQ ID NO: 8 is the human homologous IncRNA of the mouse IncRNA Gm 17499 (SEQ ID NO: 21 ). The anti-hypertrophic nature of IncRNA Gm 7499 is demonstrated in the example herein below by independent test systems. The figures show:

Figure 1 : Verification of IncRNA Gm1 1641 expression in heart samples. Mouse heart RNA was treated with DNAse I prior reverse transcription. cDNA synthesis was performed either with OligodT(20) or random primer sets.

Figure 2: (A) Validation of candidate IncRNA Gm1 1641 (alias Chast) in whole heart samples of sham and TAC mice 6 weeks post surgery by RT-PCR. FC - fold change. *p<0.05. n = 5. (B) Validation of the transcript of Gm11641 (alias Chast) by rapid amplification of cDNA ends (RACE). This graph shows the results from 3' and 5'RACE in RNA from mouse hearts including the primer sets used for each approach.

Figure 3: Organ expression of candidate IncRNA Gm1 1641. FC - fold change. Figure 4: Expression of candidate IncRNAs in cardiomyocytes, cardiac fibroblasts and endothelial cells derived from mouse hearts. FC - fold change.

Figure 5: Expression of IncRNA Gm11641 in cardiomyocytes, cardiac fibroblasts, or endothelial cells 6 weeks after TAC operation. FC - fold change. *p<0.05.

Figure 6: Subcellular localization of candidate IncRNAs. β-Actin (ActB), GAPDH, Xist and Neatl were analyzed as controls.

Figure 7: Representation of the lentiviral overexpression plasmid, designated as pLV+, harboring the full transcript sequence of a IncRNA (e.g. in this case IncRNA Gm17499).

Figure 8: Lentivirus-mediated overexpression of IncRNA Gm11641. FC - fold change. ***p<0.001 .

Figure 9: Repression of IncRNA Gm1 1641 in HL-1 cells. Expression levels have been evaluated after 48 h of incubation time. FC - fold change. *p<0.05, **p<0.01 , ***p<0.001 , n.s. = not significant. Figure 10: LncRNA Gm1 1641 expression in HL-1 cells upon hypertrophic stimulation with phenlyephrine (PE) and isoproterenol (ISO). Expression levels have been evaluated after 48 h of stimulation. **p<0.01. Figure 11 : Lentivirus-mediated overexpression and GapmeR-based silencing and of IncRNA Gm11641 in HL-1 cells stimulated with phenlyephrine (PE) and isoproterenol (ISO). ^***p<0.001 , ^*p<0.05. Figure 12: Expression of arterial natriuretic peptide (ANP) under hypertrophic conditions and deregulation of IncRNA Gm11641. ^*p<0.05, n.s. = not significant

Figure 13: (A) Representation of potential homologs of the IncRNA Gm11641 (ENSMUST00000130556) in different species applying the UCSC genome browser. (B) LncRNA Gm11641 homolog in humans. Detailed sequence and structure alignment of the murine Gm 641 and its homologs in rat und humans (upper). Validation of the human transcript by gene-specific PCR (lower left; n=at least 3 independent experiments). Expression of the human Gm11641 homolg of SEQ ID NO: 1 in healthy donor heart tissues (n=23) or in patients with aortic stenosis (n=21 ). *p<0.05. FC = fold change.

Figure 14: Validation of candidate IncRNAs in whole heart samples of TAG hearts compared to tissue of sham animals. FC - fold change. ). ^***p<0.001 , *p<0.05, n.s. = not significant.

Figure 15: (A) Validation of candidate IncRNAs derived Arraystar Mouse LncRNA microarray V2.0 comparing a cardiomyocyte (CMC) samples from 12 week healthy or 13 week sham vs. 13 week TAC mouse hearts. Candidate AJ409495 is potentially protein coding and will not be considered in the following presentation of the results. FC - fold change. CMC - cardiomyocytes. ^***p<0.001 , *^*p<0.01 , *p<0.05, n.s. = not significant. (B) Microarray validation of H19 repression due to cardiac hypertrophy in whole heart samples after several time points post TAC (A7=4-8). ^*p<0.05; ^**p<0.01. FC = fold change. (C) Expression levels of H19 in mouse hearts 2 weeks after continuous angiotensin II (ATM) infusion. (n=4-5). ^*p<0.05. FC = fold change.

Figure 16: Organ expression of candidate IncRNAs. FC - fold change.

Figure 17: Expression of candidate IncRNAs in cardiomyocytes, cardiac fibroblasts and endothelial cells derived from mouse hearts. FC - fold change.

Figure 18: Subcellular localization of candidate IncRNAs. β-Actin, GAPDH, Xist and Neatl were analysed as controls. Figure 19: Representation of the lentiviral overexpression plasmid, designated as pLV+Gm17499, harboring the full transcript sequence of the IncRNA Gm17499.

Figure 20: Lentivirus-mediated overexpression of IncRNA Gm17499. FC - fold change.

**p<0.01.

Figure 21 : Test of different antisense chemistries to suppress candidate IncRNA Gm 17499. Expression levels have been evaluated after 48 h of incubation time. FC - fold change. ^*p<0.05, **p<0.01 , ^***p<0.001 , n.s. = not significant.

Figure 22: SiRNA-based silencing and lentivirus-mediated overexpression of IncRNA Gm 17499 in HL-1 cells stimulated with phenylephrin (PE) and isoproterenol (ISO). ^***p<0.001 , ^**p<0.01 , *p<0.05, n.s. = not significant, a.u. = arbitrary unit. Scale bar in the picture represents 50 μΜ.

Figure 23: Expression of hypertrophy-associated genes under hypertrophic conditions and deregulation of IncRNA Gm17499. First row represents expression of ANP, the second that of BNP and the last row shows Mcipl .4 levels. ^*p<0.05, n.s. = not significant. Figure 24: Validation of candidate IncRNA H19 expression 4 and 6 weeks after TAC operation. ^**p<0.001 , ^*p<0.05. n = 5-7

Figure 25: LncRNA H19 expression in primary neonatal rat cardiomyocytes (NRC ) after 72h treatment with pro-hypertrophic phenylephrine (PE) and isoproterenol (ISO) or Angiotensin II (ATM), n.s. - not significant. *p<0.05.

Figure 26: (A) Repression of IncRNAs H19 in the cardiomyocyte cell lines HL-1 (mouse) and H9C2 (rat) applying esiRNA (48 h after treatment). RLUC - esiRNA against renilla luciferase (negative control), H19 - esiRNA against H19. *p<0.05. (B) Repression of H19 in HL-1 cardiomyocytes by esiRNAs compared to a non-targeting control (renilla luciferase, RLUC) (C) Overexpression of H19 applying lentiviral transduction (pLV). n=at least 3 independent experiments. ^**p<0.01 ; ***p<0.001. FC = fold change.

Figure 27: (A) Cell size of murine cardiomyocyte cell line HL-1 repressing H19 (by esiRNA) treated with the hypertrophic stimuli phenylephrine (PE) and isoproterenol (ISO) or Angiotensin II (ATI!) for 48h. RLUC - esiRNA against renilla luciferase (negative control), H19 - esiRNA against H19. ^*p<0.05. n.s. - not significant. (B) HL-1 cardiomyocyte response upon lentiviral overexpression (pLV+) of H19. Expression levels of atrial and brain natriuretic peptide (ANP, BNP) as well as β-myosin heavy chain (β-MHC) have been determined. n=at least 3 independent experiments. **p<0.01 , ^***p<0.001. FC = fold change.

Figure 28: Cell size of rat cardiomyocyte cell line H9C2 repressing H19 (by esiRNA) treated with the hypertrophic stimuli phenylephrine (PE) and isoproterenol (ISO) or Angiotensin II (ATM) for 48h. RLUC - esiRNA against renilla luciferase (negative control), H19 - esiRNA against H19. ***p<0.001 , **p<0.01. n.s. - not significant.

Figure 29: Cell size of primary neonatal rat cardiomyocytes (NRCM) repressing H19 (by esiRNA) treated with the hypertrophic stimuli phenylephrine (PE) and isoproterenol (ISO) or Angiotensin II (ATM) for 48h. RLUC - esiRNA against renilla luciferase (negative control), H19

- esiRNA against H19. ^***p<0.001 , ^**p<0.01. n.s. - not significant.

Figure 30: Cell size of primary neonatal rat cardiomyocytes (NRCM) repressing H19 (by esiRNA) treated with the hypertrophic stimuli phenylephrine (PE) and isoproterenol (ISO) or Angiotensin II (ATI!) for 72h. RLUC - esiRNA against renilla luciferase (negative control), H19

- esiRNA against H19. ^***p<0.001. n.s. - not significant.

Figure 31 : H19 expression analysis in human healthy (n=22) and hypertrophic (aortic stenosis; n=23) heart tissues by real time PCR (RT-PCR). AS: aortic stenosis. *^* p<0.01

Figure 32: Representation of conservation of the H19 sequence in different species applying the UCSC genome browser.

Figure 33: Sequence comparison of H19 sequences by Dotplot analysis. A strong conservation among mouse and rat, as well as mouse and humans can be observed. A homolog also exists in pig.

Figure 34: TAC operation in H19 knockout mice and their wt !ittermates. After 6 weeks an induction of the heart-to-body-weight ratio was observed. n=2-8. HW = heart weight, BW = body weight, Wt = wild type. Figure 35: Overexpression by AAV9-H19 in vivo for 6 weeks leads to a reversion of the hypertrophic gene program (2+E12 copies per animal). n=3. ^*p<0.05. FC = fold change. Figure 36: Potential transcriptional regulators of Gm11641 expression. Bioinformatic prediction of transcription factors binding sites in the Gm1 1641 promoter. This includes also the pro- hypertrophic transcription factor NFAT (nuclear factor of activated T-cells). In calcineurin transgenic mice (CnA TG), which have a constitutively activated NFAT pathway (n=4), Gm11641 expression is induced, while the NFAT inhibitor 1 R-VIVIT represses the expression of Gm1 1641 in HL-1 cardiomyocytes (n=at least 3 independent experiments). *p<0.05; FC = fold change, Gm1 1641 is referred to in Figure 36 as "Chast" (cardiac hypertrophy associated transcript) Figure 37: Cardiac specific overexpression of Gm1 1641 in mice applying AAV9 (6 weeks post injection). Depiction of the AAV9 construct (left) and the overexpression efficiency in mouse hearts compared to the control vector (right). n=3. *p<0.05; FC = fold change, Gm11641 is referred to in Figure 36 as "Chast". Figure 38: Overexpression by AAV9-Gm1 1641 in vivo for 6 weeks leads to cardiac hypertrophy (0.5+E12 copies per animal). This led to an induction of the heart-to-body-weight ratio and the left ventricular mass as well as an enlargement of the cardiomyocyte diameter. n=3. *p<0.05. LV mass = left ventricular mass, DAP I = 4',6-diamidino-2-phenylindole (DNA stain); WGA = wheat germ agglutinin (membrane stain), a.u. = arbitrary unit, Gm11641 is referred to in Figure 36 as "Chast".

Figure 39: TAC operation and overexpression of Gm1164 by AAV9 (0.5+E12 copies per animal) for 6 weeks led to an induction of the heart-to-body-weight ratio. n=5-8. The examples illustrate the invention:

Example 1- The pro-hvpertrophic IncRNA Gm11641

1.1 IncRNA profiling and validation

To identify IncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) a general IncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0). From this platform the IncRNA Gm1 1641 (ENSMUST00000130556) was identified as a candidate (Tab. 1 ). Tab. 1 : LncRNA Gm11641 derived from the Ncode™ Mouse Non-coding RNA Microarray comparing whole heart samples of 6 week sham and 6 week TAC mice.

The expression of this IncRNA in heart was verified via polymerase chain reaction (PGR) in cDNA derived from heart RNA that was treated with DNAse I prior reverse transcription with OligodT(20) or random primer sets (Fig. 1 ). For further validation of the transcript, the resulting PCR band was excised and sequenced. The upregulation of candidate IncRNA Gm11641 after TAC surgery was validated by real-time PCR (RT-PCR) (Fig. 2 A). For further validation of the transcript, the resulting PCR band was excised and sequenced. In a second step, the transcript sequence was analysed by rapid amplification of cDNA ends (RACE) with RNA of mouse hearts as starting material. This led to a validation of both exons (Fig 2 B).

Gm11641 was overexpressed in HL-1 cells and assessed small peptides by mass spectrometry. No peptide that overlaps to a potential short open reading frames were found, showing that Gm11641 is a non-coding RNA. .2 Organ expression of candidate IncRNA Gm11641

To determine the abundance and tissue specific expression of IncRNA Gm11641 in different organs (Fig. 3), its expression was measured in 14 different tissue samples including heart, aorta, plasma, bone marrow, skeletal muscle, lung, liver, spleen, kidney, brain, lymph node, thymus, gall bladder, and skin.

LncRNA Gm11641 is expressed in nearly all organs tested. This includes heart and plasma suggesting this transcript as a potential therapeutic target and diagnostic biomarker. 1.3 Expression of candidate IncRNA Gm11641 in cellular fractions of the heart

The expression profile of the IncRNA Gm11641 was examined in the three main cell types of the heart: cardiomyocytes, cardiac fibroblasts and endothelial cells (Fig. 4). Therefore, adult mouse heart cells were isolated from several individual hearts applying retrograde perfusion and enzymatic dissociation protocols and determined the levels of IncRNA candidates in each fraction. LncRNA Gm1 1641 is expressed in all cardiac cell types.

1.4 LncRNA Gm11641 in cellular heart fractions after TAG

Since IncRNA Gm11641 is expressed in all cardiac cells, it was aimed to identify the specific cell type that contributes to the hypertrophy-induced upregulation of IncRNA Gm11641 observed in whole heart samples (Fig. 2). Cell fractionation experiments using hearts after sham and TAG surgery (6 weeks post operation) showed that this upregulation of IncRNA Gm11641 is specifically observed in cardiomyocytes, but not in cardiac fibroblasts or endothelial cells (Fig. 5), indicating a potential role of IncRNA Gm1 1641 in cardiomyocyte hypertrophy.

1.5 Subcellular localization of candidate IncRNA Gm1 1641

The biological function of IncRNAs is strongly determined by their subcellular localization. Therefore, a biochemical separation of the total RNA derived from HL-1 cells (a cardiomyocyte cell line) into cytoplasmatic, nuclear-soluble and chromatin-associated fraction was performed (according to: Cabianca et al Cell. 1 ; 149(4):819-31.). The relative abundance of IncRNA Gm11641 in the different fractions was measured by RT-PCR. The known housekeeping genes GAPDH and β-Actin as well as the IncRNAs Xist and Neatl were used as controls for cytoplasmatic localization or chromatin-bound enrichment, respectively (Fig. 6).

As expected the housekeeping mRNAs Gapdh and β-actin (ActB) were predominantly found in cytosol, while known epigenetic modulators such as Xist and Neatl IncRNAs were predominantly found associated with chromatin. Compared to cytoplasmatic or chromatin- associated transcripts, IncRNA Gm11641 seems to be present in all subcellular fractions (cytosol, nuclear soluble and chromatin associated), suggesting a potential role in all cellular compartments. This furthermore indicates that IncRNA Gm1 1641 may modulate both transcriptional and post-transcriptional processes. 1.6 Qverexpression of candidate IncRNA Gm11641

To stably overexpress IncRNA Gm11641 , the full length transcript derived from the corresponding database (see Tab. 1) was cloned into the multiple cloning site (MCS) of a lentiviral overexpression vector (kindly provided by A. Schambach, Institute of Experimental Hematology, MHH). This plasmid harbours a bidirectional promoter that allows the production of the IncRNA transcript from the same gene regulatory element, but physically decoupled from the indicator gene GFP (green fluorescent protein) and a selection cassette. By lentiviral transduction the construct was introduced into HL-1 cardiomyocytes (see Fig. 7).

Lentivirus-mediated transduction of HL-1 cells showed a stable overexpression of IncRNA Gm11641 compared to cells harbouring the empty vector (pLV+empty) or lacking the construct (untransduced) (Fig. 8). .7 Repression of IncRNAs

To downregulate IncRNA Gm11641 applied GapmeR antisense oligonuleotides. LNA™ GapmeRs (Exigon) contain a central stretch of DNA monomers flanked by blocks of modified nucleotides (LNA, locked nucleic acids). The DNA gap activates the RNAse H-mediated degradation of targeted RNAs and is also suitable to target transcripts directly in the nucleus. With this technology the IncRNA Gm11641 was successfully downregulated in the cardiomyocyte cell line HL-1 (Fig. 9).

1.8 Functional characterization of candidate IncRNA Gm11641

1.8.1 In vitro effect of hypertrophied cardiomyocytes on Gm11641 levels

Cardiomyocyte hypertrophy is an adaptive response on the cellular level to pressure or volume stress in the heart. In vitro hypertrophic growth can be induced by stimuli including phenylephrine (PE) and isoproterenol (ISO). Therefore, HL-1 cells were stimulated with both compounds and investigated the influence on the expression of Gm11641 (Fig. 10).

1.8.2 Influence of Gm11641 deregulation on cardiomyocyte size

The hallmark of hypertrophied cardiomyocytes is an increase in cell size, relative to non- hypertrophic cells. Therefore, the cell size of HL-1 cells was investigated after stimulation with PE and ISO as well as the repressed and elevated expression of IncRNA Gm1 1641 applying the deregulation tools described above. Results are exemplary given in Fig. 11.

Under basal conditions, the overexpression of IncRNA Gm11641 leads to an increase of cell size measured by cell surface area, showing that this transcript is sufficient to induce cardiomyocyte hypertrophy. In accordance, GapmeR-based repression of IncRNA Gm1 1641 reduced cardiomyocyte size and further attenuated the PE/ISO-induced increase of cell size. These results demonstrate a pro-hypertrophic function of the IncRNA Gm1 1641. 1.8.3 Influence on hypertrophy-associated genes

Hypertrophy-induced cardiomyocyte growth is accompanied by reinduction of the "fetal gene program", because gene expression patterns mimic those seen during embryonic development. Applying the same conditions (stimulus and deregulation) regarding the candidate IncRNA Gm1 1641 expression levels of hypertrophy-associated genes including ANP, BNP (arterial and brain natriuretic peptide), Mcipl .4 (modulatory calcineurin-interacting protein 1 , exon 4 isoform), a- and β-MHC (myosin heavy chain) were measured. Fig. 12 represents the result for ANP, while the measurements of the other genes are not shown. Comparable to the cell size measurement, overexpression of IncRNA Gm11641 leads to an expression induction of the hypertrophy indicator ANP, while the knockdown augmented PE/ISO-induced ANP elevation, supporting that IncRNA Gm11641 acts as a pro-hypertrophic transcript. 1.8.4. icroarry expression data

In order to identify cardiac relevant genes that are modulated by Gm1 1641 , global microarray mRNA expression analysis was performed upon lentiviral over-expression or GapmeR- mediated suppression of Gm1 1641 (alias Chast) in the murine HL-1 cardiomyocyte cell line. Results indicated that either Gm1 1641 up-regulation or suppression have a strong effect on HL-1 cardiomyocyte transcriptome. A number of cardiac relevant genes such as Axl, Pak3, Myo18B, Egr1 , Ogn and Noslap (Tab. 2) are reciprocally regulated after Gm1 1641 over- expression and suppression. These results indicate that deregulation of Gm1 641 expression effects the transcriptional mRNA expression profile and activates pro-hypertrophic pathways in cardiomyocytes. Tab. 2: Microarray results of reciprocal regulated mRNAs upon Gm1 1641 silencing (GapmeR) and overexpression (pLV+). FC = fold change.

1.9 Conservation

To identify the homolog of IncRNA Gm1 1641 in further species including humans, BLAST was applied, being an algorithm for the comparison of primary DNA sequences with a library of a sequence database. Results of this alignment lead to the following results (Tab. 2):

Tab. 3: BLAST-based sequence comparison of transcript Gm1 1641 with different species.

The UCSC genome browser was used to depict an alignment across species (Fig. 13A). The alignment identified the human homolog of the mouse IncRNA Gm1 1641.

A more detailed sequence and structure alignment of Gm11641 revealed that Gm1 1641 homologs exist in rat, pig and humans located in intron of a protein coding gene Plekhm in antisense orientation (Figure 13B). The human homolog is annotated as: gi|528476558|ref|NC_018928.2|:62843244-62843536 Homo sapiens chromosome 17, alternate assembly CHM1_1.1., or

gi|568815581 :64783259-64783551 Homo sapiens chromosome 17, GRCh38.p2 Primary Assembly Presence of a Gm1 1641 homolog in human heart tissues was verified by gene-specific PGR (Fig. 13 B). Complementary DNA (cDNA) was prepared from RNA isolated from total human heart tissues. cDNA preparation reaction without reverse transcription served as internal control to rule-out any possible amplification of contaminating DNA. Next the expression of this human homolog was examined in 23 control hearts and 21 hypertrophic hearts from patients with aortic stenosis. In line with the results obtained in hypertrophic mouse hearts, the human Gm11641 homolog is also upregulated in human hypertrophic heart tissue (Figure 13B), evidencing that the findings of this study have a translational potential.

1.10 Regulation of Gm11641 expression

In cardiomyocytes, the hypertrophic response is orchestrated by growth factors and cytokines influencing several signalling cascades, especially the calcium dependent signalling. The transcription factor NFAT (nuclear factor of activated T-cells) is a central regulator in this pathway, finally leading to the activation of the pro-hypertrophic program.

Therefore, it was determined if this central regulator has an impact on the expression of Gm1 1641. Bioinformatic tools predict binding sites for several hypertrophy-related transcription factors in the Gm1 1641 promoter. This includes also NFAT binding sites (Fig. 36).

To study the influence of NFAT in more detail, the expression of Gm1 1641 was assessed in calcineurin transgenic mouse hearts and an induction of Gm11641 expression was found upon constitutively activated NFAT signalling. In vitro, inhibition of NFAT by 1 1 R-VIVIT led to reduced levels of Gm1 1641 in HL-1 cardiomyocytes. This data indicates that Gm11641 expression depends on the pro-hypertrophic NFAT pathway. 1.11 In vivo studies of Gm 1641

Pathological hypertrophy is a response to stress or disease such as hypertension, injuries of the heart muscle, heart valve stenosis, or neurohormones. This leads to an increase in heart muscle mass and finally to a thickening of the ventricular walls, especially of the left ventricle. Although the muscle mass is elevated, the pumping ability of the heart is not increased. On the contrary, the performance of the heart is disturbed and can finally lead to a complete failure.

To analyze the role of Gm11641 in vivo this transcript was overexpressed applying adenoviral vectors (AAV). AAV is small virus lacking an envelope and harboring a single-stranded DNA. To transfer the genetic material, AAV depends on a helper virus. However, transduction of AAV vectors does not result in the expression of further viral genes contributing to their low immunogenicity. Among all serotypes, AAV9 appears to be the most cardiotropic one. Therefore, an AAV9 was applied under the control of the cardiac-specific troponin T promoter to achieve an induction of Gm11641 directed to cardiomyocytes (Fig. 37).

Applying the AAV9-Gm11641 construct, a 20-fold induction of Gm11641 was achieved in whole heart samples. On the functional level, an induction of the heart to body weight ratio was observed (Fig. 38, upper left), indicating that Gm11641 overexpression increases the muscle mass of the left ventricle (Fig. 38, upper right). The size of cardiomyocytes was further analyzed and it was found that Gm11641 induces cardiomyocyte growth (Fig. 38, lower).

Further, left ventricular pressure overload was applied by TAG operation and Gm1 641 was overexpressed by AAV9 for 6 weeks. As expected, the aortic constriction led to an increase in the heart-to-body-weight ratio, as well as the simple injection of AAV9-Gm11641. Interestingly, TAG operation and AAV9-Gm 1641 administration exacerbated the induction of the heart mass (Fig 39).

1.12 Conclusion In conclusion, based on the findings in the tested cardiac hypertrophy models on the IncRNA Gm 1641 this IncRNA is of diagnostic and therapeutic value for cardiac hypertrophy.

Example 2- Identification of pro-hypertrophic and anti-hypertrophic IncRNAs 2.1 IncRNA profiling and validation

To achieve general IncRNA profiling Microarray analysis was performed applying platforms provided by Agilent/Life Technologies (NCode™ Mouse Non-coding RNA Microarrays) and Arraystar (Arraystar Mouse LncRNA microarray V2.0). Candidate transcripts were verified as being expressed in heart tissue via PGR (in cDNA samples derived from RNA treated with DNAse I or not prior reverse transcription) and the deregulation validated by real-time PGR.

The following Tables 3 and 4 and Figures 14 and 15 summarize candidate IncRNAs that have been validated from different Microarrays: Tab. 4: LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Tab. 5: LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts.

2.2 Organ expression of candidate IncRNAs

To determine the expression specificity of IncRNAs in different organs (Fig. 16), the expression of candidate transcripts was measured in 14 different tissue samples including heart, aorta, plasma, bone marrow, skeletal muscle, lung, liver, spleen, kidney, brain, lymph node, thymus, gall bladder, and skin. Most IncRNAs are abundant in several organs. The transcripts Gm8459, H19 and Gm 12224-1 seem to be most specifically enriched in muscle tissue including the heart. 2.3 Expression of candidate IncRNAs in cellular fractions of the heart

The expression profile of candidate IncRNAs was examined in the three main cell types of the heart: cardiomyocytes, cardiac fibroblasts and endothelial cells (Fig.17). Therefore, adult mouse heart cells were isolated from several individual hearts applying retrograde perfusion and enzymatic dissociation protocols and determined the levels of IncRNA candidates in each fraction.

2.4 Subcellular localization of candidate IncRNAs To further elucidate the action mechanism of the candidate IncRNAs, the subcellular localization was analysed by biochemical separation of the total RNA derived from HL-1 cells (a cardiomyocyte cell line) into cytoplasmatic, nuclear-soluble and chromatin-associated fraction, (according to: Cabianca et al Cell. 11 ;149(4):819-31.) The relative abundance of the transcripts in the different fractions was measured by RT-PCR. The known genes GAPDH and β-Actin as well as Xist and Neatl were run as controls for cytoplasmatic localization or chromatin-bound enrichment, respectively (Fig. 18).

More than half of the IncRNA candidates are found to be chromatin-associated, while nuclear soluble IncRNAs are not among the candidates. Only one transcript was enriched in the cytoplasm (Gm8459).

2.5. Overexpression of candidate IncRNAs

To stably overexpress candidate IncRNAs, the full length transcript derived from the corresponding database was cloned into the multiple cloning site (MCS) of a lentiviral overexpression vector (kindly provided by A. Schambach, Institute of Experimental Hematology, MHH). This plasmid harbours a bidirectional promoter that allows the production of the IncRNA transcripts from the same gene regulatory element, but physically decoupled from the indicator gene GFP (green fluorescent protein). By lentiviral transduction the construct was introduced into HL-1 cells (see Fig. 19). The following results are given exemplary for the IncRNA candidate Gm17499. Lentivirus-mediated transduction of HL-1 cells showed a stable overexpression of IncRNA Gm 17499 compared to cells harbouring the empty vector (pLV+empty) or lacking the construct (untransduced) (Fig.20). 2.6 Repression of IncRNAs

To downregulate the IncRNA transcripts three different antisense chemistries were applied: siRNAs, esiRNAs, and GapmeRs. SiRNAs (small interfering RNAs) are, similar to microRNAs, short molecules (20-25 nucleotides) that are involved in the RNA interference pathway. They bind to complementary nucleotide sequences and induce their degradation by a cytoplasmatic localized machinery. EsiRNAs (Eupheria Biotech/Sigma-Aldrich) are endoribonuclease- prepared siRNAs resulting from cleavage of long double-stranded RNAs. This antisense species targets not only one specific sequence as it is the case for siRNAs, but several sequences all over the target. LNA™ GapmeRs (Exigon) contain a central stretch of DNA monomers flanked by blocks of modified nucleotides (LNA, locked nucleic acids). The DNA gap activates the degradation of target RNA and is suitable to target transcripts directly in the nucleus.

Exemplary, the results for Gm 17499 applying all three chemistries are shown (Fig. 21 ) as well as for H19. H19 was successfully downregulated with esiRNA and alatl and was repressed applying GapmeRs (this IncRNA served as positive control for the GapmeR technology).

2.7 Functional characterization of candidate IncRNAs 2.7.1 Influence on cardiomyocyte size

Cardiomyocyte hypertrophy is an adaptive response on the cellular level to pressure or volume stress in the heart. In vitro hypertrophic growth can be induced by stimuli including phenylephrine (PE) and isoproterenol (ISO). Therefore, HL-1 cells were stimulated with both compounds and investigated cardiomyocyte cell size under altered IncRNA levels. Results are exemplary given for the IncRNA Gm 17499 (Fig. 22).

Enhanced expression of IncRNA Gm 17499 prevents cell size increase due to pro-hypertrophic stimuli, while its silencing resulted in an enlargement of cardiomyocytes, indicating an anti- hypertrophic function of this transcript. 2.7.2 Influence on hypertrophy-associated genes

Hypertrophy-induced cardiomyocyte growth is accompanied by a reinduction of the "fetal gene program", because gene expression patterns mimic those seen during embryonic development. Applying the same conditions (stimulus and deregulation) regarding the candidate IncRNA Gm 17499 expression levels of hypertrophy-associated genes including ANP, BNP (arterial and brain natriuretic peptide), and Mcip1.4 (modulatory calcineurin- interacting protein 1 , exon 4 isoform) were measured (Fig. 23). 2.8. Conclusion

In conclusion, based on the above experimental findings the deregulated IncRNAs in the tested cardiac hypertrophy model (left ventricular pressure overload) are of diagnostic and therapeutic interest for cardiac hypertrophy. In particular, the following IncRNAs are of major importance:

Tab. 6: Summary of investigated IncRNAs candidates.

Name Identifier Source Size relationship related

gene

Gm17499 ENSMUST00000171177 Ensembl 692 Antisense Tmpo

bp

Gm16192 ENSMUST00000 48357 Ensembl 937 antisense Mtusl

bp

Gm11641 ENSMUST00000130556 Ensembl 923 Antisense

bp

Gm8459 ENSMUST00000162504 Ensembl 760 intergenic

bp

AK083183 AK083183.1 Fantom3 2759 intergenic

bp

Gm13316 ENSMUST00000140537 Ensembl 691 antisense Cacnb2

bp

H19 ENSMUST00000136359 Ensembl 2286 intergenic

(NR_001592.1) bp

ENSMUST00000152754 Ensembl 1853 intergenic

bp ENSMUST00000140716 Ensembl 817 Intergenic

bp

AK013700 AK013700.1 predicted 725 intergenic

bp

Gm15892-002 ENSMUST00000152627 Ensembl 439 intergenic

bp

BC023483 uc007dvi.1 UCSC 1726 intergenic

bp

Gm12224-001 ENSMUST00000124047 Ensembl 567 antisense Acsl6 bp

AJ409495 AJ409495.1 NRED 292 intergenic

bp

Gm8822-202 ENSMUST00000168371 Ensembl 339 intergenic

bp

Table 7: Human homologs of the the investigated IncRNAs candidates

Mouse Identifier human homolog Identity IncRNA to

mouse sequenc e

Gm 17499 ENSMUST000001711 >gi|568815586|ref|NC_000012.12|: 88.42%

77 98547558-98547816 Homo

sapiens thymopoietin (TMPO), chromosome 12, GRCh38 Primary

Assembly

Gm16192 ENSMUST000001483 >gi 117224595|gb|AF293357.11 : 128 78.47%

57 -394 Homo sapiens AT2 receptor- interacting protein 1 mRNA, complete cds

Gm11641 >gi|568815581 :64783259- 69%

64783551 Homo sapiens chromosome 17, GRCh38.p2

Primary Assembly Gm8459 ENSMUST000001625 >gi|307219258|ref|NG_001223.5|:1 80.52% 04 49-953 Homo sapiens voltage- dependent anion channel 1 pseudogene 2 (VDAC1 P2) on chromosome X

AK08318 AK083183.1 >gi|568815595|ref|NC_000003.12|: 69.10%

3 142958129-142958893 Homo

sapiens chromosome 3, GRCh38

Primary Assembly

Gm13316 ENSMUST000001405 >gi|69122714|ref|NM_014409.3|:1 87.37%

37 241-1929 Homo sapiens TAF5-like

RNA polymerase II, p300/CBP- associated factor (PCAF)- associated factor, 65kDa (TAF5L), transcript variant 1 , mRNA

H19 NR_001592.1 * 71.25%

>gi|57862814|ref|NR_002196.1 |:70

8-2305 Homo sapiens H19, imprinted maternally expressed transcript (non-protein coding)

(H19), long non-coding RNA

AK01370 AK013700.1 >gi|13899395|gb|AC008383.8|AC0 71.98% 0 08383:167188-167922 Homo

sapiens chromosome 5 clone

CTC-222022, complete sequence

Gm15892 ENSMUST000001526 >gi|21754306|dbj|AK095112.11:368 80.00% -002 27 1-4000 Homo sapiens cDNA

FLJ37793 fis, clone

BRHIP3000473

BC02348 uc007dvi.1 >gi|568815597|ref|NC_000001.111: 74.27%

3 245708650-245709375 Homo

sapiens chromosome 1 , GRCh38

Primary Assembly

Gm12224 ENSMUST000001240 >gi|568815593|ref|NC_000005.10|: 88.64% -001 47 131971540-131971671 Homo sapiens chromosome 5, GRCh38

Primary Assembly

AJ409495 AJ409495.1 >gi|568815593|ref|NC_000005.10|: 89.17%

123761272-123761427 Homo

sapiens chromosome 5, GRCh38

Primary Assembly

Gm8822- ENSMUST000001683 >gi|27882035|gb|BC044590.11:321 94.10%

202 71 -659 Homo sapiens ARP3 actin- related protein 3 homolog (yeast),

mRNA (cDNA clone MGC:57216

IMAGE:5261640), complete cds

Example 3 - Further characterization of the anti-hvpertrophlc IncRNA H19

3.1. Differential expression in 4 and 6 week sham/TAC

To identify IncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general IncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0). From this platform the IncRNA H19 (NR_001592.1 ) was identified as one of the potential candidates in addition to Gm 17499 and Gm11641 (Tab. 8 and 9).

Tab. 8: H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Tab. 9: H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. Name identifier Sourc Size relations FC Regula

e hip tion

H19 ENSMUST00000 Ensem 817 intergeni Down

(NR_0015 140716 bl bp c 4,73

92.1 ) ENS UST00000 Ensem 2286 intergeni Down

136359 bi bp c 2,48

The downregulation of candidate IncRNA H19 four and six weeks after TAG surgery was validated by real-time PGR (RT-PCR) (Fig. 24A and B; validation of the cardiomyocyte- specific array is shown above (Table 8 and Fig. 15 A and B). The repression of H19 was verified in whole heart samples 6 weeks after TAC operation. In addition, this repression was observed in earlier as well as later stages of hypertrophy and heart failure (Fig. 15 B). The validation of the CMC-specific array revealed, that this repression can be found in cardiomyocytes (Fig. 15 A), suggesting that H19 might be involved in the development of cardiac hypertrophy and modulation of H19 levels is of therapeutical value.

A second model of cardiac hypertrophy is the continuous infusion of angiotensin II (ATM). This compound is a central product of the renin-angiotensin system and causes an increase in blood pressure via its vasoconstrictive effect. Clinical studies on the effect of angiotensin- converting enzyme (ACE) inhibitors revealed that ATM plays also a central role in the pathophyiology of cardiac hypertrophy, remodeling and heart failure. To induce cardiac hypertrophy, osmotic minipumps (Alzet pumps) are implanted subcutaneously into mice for 2 weeks. In this model, the same reduction of H19 levels was observed as for the TAC model, underlining the relevance of this IncRNA in cardiac hypertrophy (Fig. 15 C). 3.2 Organ expression of candidate IncRNA H19

To determine the abundance and tissue specific expression of IncRNA H19 in different organs (Fig. 16), its expression was measured in 14 different tissue samples including heart, aorta, plasma, bone marrow, skeletal muscle, lung, liver, spleen, kidney, brain, lymph node, thymus, gall bladder, and skin. H19 shows a strong enrichement in muscle tissue, including heart, skeletal muscle, and gal bladder. 3.3 Expression of candidate IncRNA H19 in cellular fractions of the heart

The expression profile of IncRNA H19 was examined in the three main cell types of the heart: cardiomyocytes, cardiac fibroblasts and endothelial cells. Therefore, adult mouse heart cells were isolated from several individual hearts applying retrograde perfusion and enzymatic dissociation protocols and determined the levels of IncRNA candidates in each fraction. H19 was found to be expressed in all cardiac cell types (Fig. 17).

3.4 Subcellular localization of candidate IncRNA H19

The biological function of IncRNAs is strongly determined by their subcellular localization. Therefore, a biochemical separation of the total RNA derived from HL-1 cells (a mouse cardiomyocyte cell line) into cytoplasmatic, nuclear-soluble and chromatin-associated fraction was performed (according to: Cabianca et al Cell. 11;149(4):819-31.). The relative abundance of IncRNA H19 in the different fractions was measured by quantitative RT-PCR. The known housekeeping genes GAPDH and β-Actin as well as the IncRNAs Xist and Neatl were used as controls for cytoplasmatic localization or chromatin-bound enrichment, respectively. H 9 is found in all subcellular fractions, the cytosol, nuclear soluble and chromatin associated compartments of cardiomyocytes (Fig. 18). This indicates that H19 has the possibility to modulate both transcriptional and post-transcriptional processes.

3.5 Repression of candidate IncRNA H19

To downregulate H19 IncRNA an antisense chemistry named esiRNA was applied. EsiRNAs (Eupheria Biotech/Sigma-Aldrich) are endoribonuclease-prepared siRNAs resulting from cleavage of long double-stranded RNAs. This antisense species targets not only one specific sequence as it is the case for siRNAs, but several sequences all over the target.

Compared to a control transfection (RLUC, renilla luciferase), IncRNA H19 was significantly downregulated in two different cardiomyocyte cell lines derived from mouse (HL-1 ) and rat (H9C2) (Fig. 26 A and B).

To stably overexpress this transcript, the full length transcript was cloned into the multiple cloning site (MCS) of a lentiviral overexpression vector. This plasmid harbours a bidirectional promoter that allows the production of the IncRNA transcript from the same gene regulatory element, but physically decoupled from the indicator gene GFP (green fluorescent protein) and a selection cassette. By lentiviral transduction the construct was introduced into HL-1 cardiomyocytes. Lentivirus-mediated transduction of HL-1 cells showed a stable overexpression of H19 compared to cells harbouring the control vector (pLV+empty) (Fig. 26 C).

3.6 Functional characterization of IncRNA H19

3.6.1 In vitro effect of hypertrophied cardiomyocytes on H19 levels Cardiomyocyte hypertrophy is an adaptive response on the cellular level to pressure or volume stress in the heart. In vitro hypertrophic growth can be induced by stimuli including phenylephrine (PE) and isoproterenol (ISO) or Angiontensin II (ATM). Therefore, HL-1 cells were stimulated with these compounds and investigated their influence on the expression of H19 (Fig. 25).

3.6.2 Influence of H19 repression on cardiomyocyte size

The hallmark of hypertrophied cardiomyocytes is an increase in cell size, relative to non- hypertrophic cells. Therefore, the cell size of cardiomyocytes was investigated after stimulation with PE and ISO or ATM while repressing H19 with esiRNA. The knockdown of H19 resulted in a significant increase in cardiomyocyte size in rat H9C2 cell line after 48 h as well as in neonatal rat cardiomyocytes (NRCMs) after 72 h (Fig. 28 to 30). This effect was observed after treatment with PE and ISO in both rat cardiomyocyte cell line as well as in NRCMs after AT II treatment. In the HL-1 cell line a comparable trend was observed (Fig. 27A).

Moreover, H19 was overexpressed in HL-1 cells. Overexpression of H19 in HL-1 cardiomyocytes led to a decrease of cardiac stress markers atrial and brain natriuretic peptide (ANP, BNP) and reduced levels of β-MHC (β-myosin heavy chain), another prominent marker of cardiac hypertrophy (Fig. 27 A), further proving that induction of H19 has a cardioprotective effect.

3.7 Analysis of H19 expression in healthy and diseased human heart tissues Aortic stenosis refers to calcification of aortic valve, which inhibits flow of blood. As a result heart needs to pump blood at a high pressure, finally leading to hypertrophic growth of the heart muscle. H19 expression was measured in 22 healthy heart tissues and 23 hypertrophic (aortic stenosis) heart tissues and found that H19 expression is strongly reduced in hypertrophic hearts (Fig. 31 ). This data from human subjects corroborates the findings described herein in mouse and rat in vitro models, and further evidences that H19 is a bona fide therapeutic target to prevent cardiac hypertrophy.

3.8. Evolutionary conservation of H19

Several studies have shown that the H19 sequence as well the H19-imprinting mechanism are highly conserved among humans and rodents. This can be also depicted applying the UCSC genome browser (Fig. 32). A more detailed alignment of various H19 sequence by Dotplot analysis underlines these findings (Fig. 33) and shows a strong sequential relation between the murine and rat sequence, as well as the murine and human sequence. A further comparison revealed that the human H19 exhibits a homolog in pigs, enabling the performance of H19-related therapy strategies in large animal models.

3.9 In vivo studies on H19

To study the effects of H19 on cardiac remodeling in vivo a H19 knockout mouse was investigated. The deletion of H19 was achieved by neomycin resistance cassette that replaced the promoter and the entire 3 kb transcription unit of H19 (strain description: _{H 1 Q}tmiLda _{in a 12}9S2/SvPas background).

The H19 knockout animals and their wildtype littermates (wt) were subjected to transverse aortic constriction (Fig. 34). As expected, after 6 weeks post surgery the wt showed an induction and heart-to-body-weight ratio. This gain of heart mass was also observed for sham mice comparing wt and H19 knockout animals, while TAC-operated mice lacking the H19 gene showed an exacerbated phenotype. To get a more detailed analysis of the role of H19 in cardiac hypertrophy and to develop a potential therapeutic strategy, this transcript was overexpressed applying adenoviral vectors (AAV). An AAV9 was applied under the control of the cardiac-specific troponin T promoter to achieve an induction of H19 directed to cardiomyocytes (Fig. 35). Applying the AAV9-H19 construct, a 150-fold induction of H19 was achieved in whole heart samples. Changes on transcriptional level of specific markers of cardiac hypertrophy were analyzed. Among them are the transcripts a-MHC, which is down-regulated in cardiac hypertrophy, and β-MHC, which is up-regulated. By in vivo overexpression of H19, a reversion of the a-MHC-to- -MHC switch was observed (Fig. 35), indicating that adenoviral administration of H19 has a beneficial effect on the hypertrophic gene program and serves as a therapeutic strategy.

Claims

1. A pharmaceutical composition comprising

(i) a compound promoting the expression and/or the activity of one or more long non-coding RNAs (IncRNAs) selected from SEQ ID NOs 12, 8 to 11 and 13; and/or

(ii) a compound inhibiting the expression and/or the activity of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28.

2. A compound (i) promoting the expression and/or the activity of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13; and/or

(ii) inhibiting the expression and/or the activity of one or more IncRNAs selected from

SEQ ID NOs 1 to 7, 27 and 28

for use in treating or preventing cardiac hypertrophy.

3. The compound for use according to claim 2, wherein the cardiac hypertrophy is a ventricular hypertrophy.

4. The pharmaceutical composition or compound for use according to any one of claims 1 to 3, wherein the compound as defined in (ii) is

(a) a nucleic acid sequence which comprises or consists of a nucleotide sequence being complementary to at least 12 continuous nucleotides of a IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 29,

(b) a nucleic acid sequence which comprises or consists of a nucleotide sequence which is at least 69% identical to the complementary strand of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28,

(c) a nucleic acid sequence which comprises or consists of a nucleotide sequence according to (a) or (b), wherein U is replaced by T,

(d) an expression vector expressing the nucleic acid sequence as defined in any one of (a) to (c), preferably under the control of a heart-specific promoter, or

(e) a host comprising the expression vector of (d).

5. The pharmaceutical composition or compound for use according to any one of claims 1 to 3, wherein the compound as defined in (ii) is an aptamer, a ribozyme, an antibody, a protein drug, or a small molecule inhibitor.

The pharmaceutical composition or compound for use according to any one of claims 1 to 5, wherein the compound as defined in (i) is

(a) a nucleic acid sequence which comprises or consists of the nucleic acid sequence of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13 or an nucleic acid sequence which is at least 69% identical thereto,

(b) an expression vector expressing the nucleic acid sequence as defined in (a), preferably under the control of a heart-specific promoter, or

(c) a host comprising the expression vector of (b).

(a) a transcription factor promoting the expression of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13, and/or

(b) a small molecule enhancing the expression of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13.

A method for diagnosing cardiac hypertrophy in a patient, comprising

(a) detecting the expression level of one or more IncRNAs selected from SEQ ID NOs 12, 1 to to 1 , 13, 27 and 28 in a sample obtained from said patient, and

(b) comparing said expression level of the one or more IncRNAs with the expression level of these one or more IncRNAs in a sample obtained from healthy subjects, wherein

a greater than 2-fold downregulation of one or more IncRNAs selected from SEQ ID NOs 1 to 7, 27 and 28; and/or

a greater than 2-fold upregulation of one or more IncRNAs selected from SEQ ID NOs 12, 8 to 11 and 13

is indicative for a cardiac hypertrophy in the patient.

The method according to claim 8, wherein said sample is a blood sample or blood- derived sample.

The method according to claim 8, wherein said sample is a heart tissue sample.

The method of any one of claims 8 to 10, wherein the detection of the expression level of the one or more IncRNAs comprises

(a) quantitative PGR, preferably quantitative real time PGR, or (b) a template/RNA amplification method followed by determining the expression level of the one or more IncRNAs using a fluorescence- or luminescence-based quantification method.

12. The method of any one of claims 8 to , wherein the method comprises prior to the detection of the expression level of the long non-coding RNA a pre-amplification step of the RNA within the test patient's sample and/or the control patient's sample.

13. A kit for diagnosing cardiac hypertrophy in a patient, said kit comprising means for the detection of the expression level of one or more IncRNAs selected from SEQ ID NOs

12, 1 to 11 , 13, 27 and 28, and instructions how to use the kit.

14. The kit of claim 13, wherein the means are primer pairs used for the specific detection of the expression level of one or more IncRNAs selected from SEQ ID NOs 12, 1 to 11 ,

13, 27 and 28.

15. The pharmaceutical composition, compound for use, method or kit of any one of claims 1 to 14, wherein the one or more IncRNAs are at least 3 IncRNAs, and preferably at least 5 IncRNAs.