WO2015145851A1 - Dispositif de détection d'analyte, système de détection d'analyte, kit de détection d'analyte, procédé de détection d'analyte, programme, et moyen d'enregistrement - Google Patents

Dispositif de détection d'analyte, système de détection d'analyte, kit de détection d'analyte, procédé de détection d'analyte, programme, et moyen d'enregistrement Download PDF

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Publication number
WO2015145851A1
WO2015145851A1 PCT/JP2014/079083 JP2014079083W WO2015145851A1 WO 2015145851 A1 WO2015145851 A1 WO 2015145851A1 JP 2014079083 W JP2014079083 W JP 2014079083W WO 2015145851 A1 WO2015145851 A1 WO 2015145851A1
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WIPO (PCT)
Prior art keywords
evaluation result
image
specimen
pattern
color development
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PCT/JP2014/079083
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English (en)
Japanese (ja)
Inventor
巌 和賀
幸司 森下
克幸 永井
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Ｎｅｃソリューションイノベータ株式会社
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Priority to JP2016509895A priority Critical patent/JPWO2015145851A1/ja
Publication of WO2015145851A1 publication Critical patent/WO2015145851A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour

Definitions

  • the present invention relates to a specimen detection device, a specimen detection system, a specimen detection kit, a specimen detection method, a program, and a recording medium.
  • an aptamer is used as a sensor to detect viable bacteria (detection target) mixed in food (specimen).
  • the following Patent Document 1 discloses that an aptamer sensor is combined with a labeling substance that develops color suitable for simple inspection, and the presence or absence of viable bacteria is determined by developing color only in the presence of viable bacteria. Yes.
  • the present invention provides a specimen detection device, specimen detection system, specimen detection kit, specimen detection method, program, and recording that can clearly understand the detection result in the color development reaction even for those who do not have specialized knowledge
  • the purpose is to provide a medium.
  • the specimen detection apparatus of the present invention comprises: A coloring pattern image acquisition means for acquiring an image including a coloring pattern of the specimen; An evaluation result storage means for preliminarily storing the color development pattern of the specimen and the evaluation result of the specimen corresponding to the color development pattern of the specimen as evaluation result information; An evaluation result image generating means for generating a corresponding evaluation result image with reference to the evaluation result information stored in the evaluation result storage means from the color development pattern of the image including the color development pattern; And a synthesized image generating unit configured to generate a synthesized image obtained by synthesizing the image including the coloring pattern and the evaluation result image.
  • the sample detection system of the present invention comprises: Including terminals and servers, The terminal and server are connectable via a communication line network,
  • the server includes the sample detection device of the present invention,
  • the terminal includes an imaging unit and an image display unit, An image including the color development pattern is captured by the imaging unit of the terminal, and the captured image is acquired by the image acquisition unit of the sample detection device via the communication network.
  • the composite image generated by the composite image generation unit of the specimen detection apparatus is displayed by the image display unit of the terminal via the communication line network.
  • the first specimen detection kit of the present invention comprises: A reagent for detecting a detection target in a sample with a color pattern and a sample detection device are included, and the sample detection device is the sample detection device of the present invention.
  • the second specimen detection kit of the present invention comprises: A reagent for detecting a detection target in a sample with a color pattern and a terminal are included, and the terminal is a terminal of the sample detection system of the present invention.
  • the specimen detection method of the present invention comprises: A coloring pattern image acquisition step of acquiring an image including a coloring pattern of the specimen; An evaluation result storage step for preliminarily storing the color development pattern of the specimen and the evaluation result of the specimen corresponding to the color development pattern of the specimen in association as evaluation result information; An evaluation result image generation step of generating a corresponding evaluation result image with reference to the evaluation result information stored in the evaluation result storage step from the color development pattern of the image including the color development pattern; And a synthesized image generating step of generating a synthesized image obtained by synthesizing the image including the coloring pattern and the evaluation result image.
  • the program of the present invention causes a computer to execute the specimen detection method of the present invention.
  • the recording medium of the present invention is a computer-readable recording medium that records the program of the present invention.
  • a sample detection device a sample detection system, a sample detection kit, a sample detection method, a program, and a record that can clearly understand the detection result in a color reaction even for those who do not have specialized knowledge
  • a medium can be provided.
  • FIG. 1 is a block diagram showing the configuration of an example of the sample detection system of the present embodiment.
  • FIG. 2 is a diagram illustrating an example of a figure recognized by the color pattern recognition unit of the sample detection apparatus of the present embodiment.
  • FIG. 3 is a flowchart of an example of the specimen detection method of the present embodiment.
  • FIG. 4 shows a process chart prior to the sample evaluation method of the present embodiment.
  • FIG. 5 shows a flowchart of the sample evaluation method of the present embodiment.
  • FIG. 6 is a flowchart showing an example of another specimen evaluation method of the present embodiment.
  • FIG. 1 is a block diagram showing the configuration of an example of the sample detection system 10 of the present embodiment.
  • the system 10 includes a terminal 1 and a server 2, and the terminal 1 and the server 2 can be connected via a communication line network 4, and the server 2 includes a sample detection device 3.
  • the communication line network 4 is not particularly limited, and a publicly known communication line network can be used. Specific examples include an Internet line, a telephone line, a LAN (Local Area Network), and the like. Good.
  • the terminal 1 includes an imaging unit 5 and an image display unit 6 as shown in FIG.
  • the imaging unit 5 captures an image including a coloring pattern.
  • the imaging unit 5 is not particularly limited, and examples thereof include a CCD (Charge Coupled Device) camera, a CMOS (Complementary Metal Oxide Semiconductor) camera, and a scanner.
  • the image display unit 6 displays the composite image generated by the composite image generation unit 14 of the specimen detection device 3. Although it does not restrict
  • the terminal 1 is not particularly limited as long as it includes the imaging unit 5 and the image display unit 6, and examples thereof include a mobile phone, a smartphone, a personal computer (PC), and a tablet terminal.
  • the specimen detection apparatus 3 mainly includes a color pattern image acquisition unit (image acquisition unit) 11, an evaluation result storage unit 12, an evaluation result image generation unit 13, and a composite image generation unit 14. Included as a component. As shown in the figure, the image acquisition unit 11 and the composite image generation unit 14 can be connected to the communication network 4.
  • the image acquisition unit 11 acquires an image including the color development pattern of the specimen.
  • the image acquisition unit 11 acquires, for example, an image including the color pattern captured by the imaging unit 5 of the terminal 1 via the communication line network 4.
  • the evaluation result storage means 12 the color development pattern of the specimen and the evaluation result of the specimen corresponding to the color development pattern of the specimen are associated and stored in advance as evaluation result information.
  • the evaluation result image generation means 13 refers to the evaluation result information stored in the evaluation result storage means 12 from the color development pattern of the image including the color development pattern, and generates a corresponding evaluation result image.
  • the composite image generation unit 14 generates a composite image obtained by combining the image including the color pattern and the evaluation result image.
  • the evaluation result storage means 12 is not particularly limited. For example, a database or server, a random access memory (RAM), a read only memory (ROM), a hard disk (HD), an optical disk, a floppy (registered trademark) disk (FD), or the like. Can be given.
  • the evaluation result storage unit 12 may be, for example, a device built-in type or an external type such as an external storage device.
  • the evaluation result image generating means 13 is not particularly limited, but preferably includes a coloring pattern recognition means.
  • the color pattern recognition means recognizes the color pattern by recognizing a preset figure from an image including the color pattern. In the present embodiment, the color pattern is used as a marker.
  • the preset figure is not particularly limited, and may be, for example, a square 16 figure composed of lines surrounding the coloring pattern 15 shown in FIG.
  • a black frame 17 surrounding the coloring pattern 15 may be used.
  • the specimen detection device 3 may further include an imaging unit and an image display unit, and may be an integrated device without being connected to the terminal 1 and the communication network.
  • the imaging unit is not particularly limited, and may be the same as the imaging unit of the terminal 1, for example.
  • the image display unit is not particularly limited, and is the same as, for example, the image display unit of the terminal 1. It may be.
  • the specimen is not particularly limited, and for example, biological samples, food (including beverages), pharmaceuticals, chemicals, soil, plants, microorganisms, viruses, water (for example, tap water, drainage, rivers) Water, seawater, rainwater, snow, etc.), garbage, waste, etc.
  • biological samples include saliva, urine, blood and the like.
  • the color development includes not only the appearance of color but also the change of color (discoloration). In addition, the color development includes that there is no color change or no color change when the detection target is not present in the specimen.
  • the color development pattern of the specimen is not particularly limited, and is, for example, a color development pattern as a result of a general color reaction between a detection target and a reagent in the specimen.
  • the color pattern includes the presence or absence of color development due to a color reaction between the detection object and the reagent. Specifically, for example, it may be a color pattern resulting from the binding of a reagent that specifically binds to the detection target in the sample, or the result of an oxidation-reduction reaction between the detection target and the reagent (indicator).
  • the coloring pattern may be used.
  • the term “one color pattern of the specimen” means, for example, a pattern of the presence or absence of color development in one spot.
  • the generated evaluation result image is an image of a comprehensive evaluation result of the plurality of color patterns.
  • the detection target in the sample is not particularly limited, and examples thereof include agricultural chemicals, food poisoning bacteria, and biomarkers.
  • examples of the biological marker include proteins, genes, antigens, and the like.
  • the reagent is not particularly limited, and examples thereof include antibodies, aptamers, and the like. Aptamers are preferable from the viewpoint that the detection target can be detected even in a trace amount. If the reagent is an aptamer, it has the ability to specifically bind to biomolecules such as proteins, and because it can be chemically synthesized, it is chemically stable and easy to handle.
  • the corresponding evaluation result image is not particularly limited, and examples thereof include a 3D image, a 2D image, text information (for example, characters, numerical values, etc.) that look like a three-dimensional image from the viewpoint of the camera.
  • the evaluation result image is an angel indicating that it is safe
  • the evaluation result image may be a skull character image indicating that it is dangerous.
  • the corresponding evaluation result image may be stored in advance in association with the evaluation result of the specimen in the storage means, for example.
  • the storage unit is not particularly limited, and may be the same as or not the same as the evaluation result storage unit 12, for example.
  • the storage means is the same as the evaluation result storage means 12, for example, it is stored in a different area from the area where the evaluation result information is stored.
  • the composite image refers to an image obtained by combining the image including the coloring pattern and the evaluation result image.
  • the composite image may be, for example, an image obtained by superimposing the evaluation result image on an image including the color pattern, or a part or all of the image including the color pattern may not be visible.
  • the evaluation result image translucent, the entire image including the coloring pattern may be visible.
  • sample evaluation method Next, the sample evaluation method of the present embodiment will be described with reference to the flowchart of FIG.
  • the sample evaluation method of this embodiment is implemented as follows using the sample evaluation system 10 of this embodiment shown in FIG. 1, for example.
  • the sample evaluation method of the present embodiment is not limited to using the system 10.
  • the color development pattern of the sample and the evaluation result of the sample corresponding to the color development pattern of the sample are associated and stored in advance in the evaluation result storage means 12 as evaluation result information (evaluation result storage step).
  • the evaluation result information may be one or plural.
  • the detection target in the sample is colored using a reagent (S1).
  • the imaging unit 5 of the terminal 1 captures an image including the color development pattern of the specimen (S2).
  • an image including the color development pattern of the specimen is acquired by the image acquisition means 11 (S3).
  • the image acquisition step (S3) for example, the captured image may be acquired by the image acquisition unit 11 via the communication network 4 by the imaging unit 5 of the terminal 1.
  • the evaluation result image generation means 13 refers to the evaluation result stored in the evaluation result storage step from the color development pattern of the image including the color development pattern, and generates a corresponding evaluation result image (S4).
  • the composite image generation unit 14 generates a composite image synthesized with the image including the color pattern and the evaluation result image (S5).
  • the composite image generated by the composite image generation means 14 is displayed by the image display means 6 of the terminal 1 via the communication line network 4 (S6).
  • the imaging means 5 such as a camera is held up so that the coloring pattern of the sample is reflected, and the processing of S2 to S6 is continuously performed on the sample by the image display means 6 of the terminal 1. It is possible to display information that is easy to understand intuitively by superimposing information in real time.
  • AR Augmented Reality
  • the color development pattern is used as a marker for image recognition, and additional information for clarifying the detection result is presented according to the color development pattern. For this reason, if the present invention is used, even a person who does not have specialized knowledge can clearly understand the detection result in the color development reaction.
  • the color pattern recognition unit of the apparatus 3 may recognize the preset color pattern.
  • the color pattern recognition means recognizes the color pattern by recognizing the preset figure from an image including the color pattern.
  • S1 to S2 and S6 are optional steps and may or may not be present.
  • a test paper 21 shown in FIG. 4A is prepared.
  • the test paper 21 is provided with spots 22A and 22B, and each spot 22A and 22B is provided with reagents A and B for detecting harmful substances from food in a colored pattern, respectively.
  • food that is a sample is added to each of the spots 22A and 22B.
  • the color changes if there is a harmful substance in the food shown in black in the figure (B)
  • the color changes not (shown in white in the figure (B)).
  • the color patterns 22A and 22B are obtained according to the color change.
  • FIG. 5B when there are two color patterns, four types of images including the color patterns can be formed.
  • the color development patterns 22A and 22B of the specimen and the comprehensive evaluation results of the specimen corresponding to the color development patterns 22A and 22B of the specimen are associated with each other as a database (evaluation result storage). Means) 12 is stored.
  • the database 12 stores four pieces of evaluation result information 23A to 23D.
  • the evaluation result information 23A indicates that the two color development patterns 22A and 22B are both white and have not changed. In this case, as shown in FIG. 5C, the evaluation result “safe” is shown.
  • one of the two color development patterns 22A and 22B is black, indicating that the color is changing in any one of the spots 22.
  • the evaluation result “danger” is shown as shown in FIG.
  • the evaluation result information 23D indicates that both of the two color development patterns 22A and 22B are black and that each of the spots 22 has changed color.
  • the evaluation result “very dangerous” is shown as shown in FIG.
  • an evaluation result image generating means refers to the comprehensive evaluation result stored in the database 12 from the color patterns 22A and 22B included in the image 34.
  • the evaluation result image generation means has the color patterns 22A and 22B that match the color patterns 22A and 22B of the evaluation result information 23A shown in FIG.
  • the overall evaluation result of the coloring patterns 22A and 22B is determined as “safe”.
  • the evaluation result image generation unit generates an image of an angel character indicating “safety” as a corresponding evaluation result image.
  • a combined image generating unit (not shown) generates a combined image obtained by combining the image 34 including the coloring patterns 22A and 22B and the evaluation result image.
  • the composite image 35 generated by the composite image generation means (not shown) is displayed on the display of the smartphone 33 via the Internet.
  • the color development step has been described using the test paper 21 but is not limited thereto.
  • a reaction plate such as a plastic or glass plate may be used instead of the test paper, and for example, DNA
  • the specimen and the reagent may be mixed using a (Deoxyribonucleic acid) chip or the like.
  • the color development step used a method of adding a sample to the reagent on the test paper 21, but the method is not limited to this.
  • the sample and the reagent are mixed in advance, You may add the said liquid mixture on a test paper.
  • an example of application to safety in the food field has been shown as an example of use of the present invention, but the example of use of the present invention is not limited to this.
  • the quality of the food is evaluated by evaluating the taste as an evaluation result image and displaying it on a terminal display or the like. It can also be used for improvement.
  • a gene as a specimen to check the sex of a fetus, for example, displaying a character image of a moth or moth as an evaluation result image on a terminal display or the like
  • a sex discriminator for a fetus that can clearly understand the evaluation result.
  • a component serving as an index of happiness, or the like as a specimen for use in a fatigue check or happiness check for example, as an evaluation result image, a facial expression indicating fatigue
  • the evaluation result can be used as a fatigue checker or a happiness checker that can clearly understand the evaluation result.
  • a lie is used as a sample
  • a component that is secreted is used as a sample
  • a character mark such as “lie” or “true” is displayed on the terminal display, etc. Therefore, it can be used as a lie detector that can clearly understand the evaluation result.
  • a specimen blood or urine containing a component that is an indicator of an infant's or pet's emotion is used as a specimen, and the infant's or pet's facial expression is displayed on a terminal display, etc. Available.
  • the evaluation result image generation means 13 includes an evaluation result estimation means, and the evaluation result estimation means is stored in the color development pattern of the image including the color development pattern and the evaluation result storage means.
  • Embodiment 1 except that the evaluation result is estimated by comparing the color development pattern of the evaluation result information, and the evaluation result image generating means generates an evaluation result image corresponding to the estimated evaluation result.
  • the sample detection system of the present embodiment has the same configuration as the sample detection system 10 of the first embodiment except that the sample detection apparatus of the present embodiment is a constituent element.
  • the sample detection method of the present embodiment includes an evaluation result estimation step in the step S4 shown in FIG. 3, and the evaluation result estimation step stores the color development pattern of the image including the color development pattern and the evaluation result storage step.
  • the step S4 in the present embodiment can be described as follows using FIG. That is, first, as shown in the figure, the database 12 stores two pieces of evaluation result information 43A and 43B.
  • the coloring patterns 22A and 22B of the evaluation result information 43A are slightly darker in any case.
  • the comprehensive evaluation result of the evaluation result information 43A is “safe”.
  • the coloring patterns 22A and 22B of the evaluation result information 43B are completely changed in dark color.
  • the comprehensive evaluation result of the evaluation result information 43B is “danger”.
  • the color development patterns 22A and 22B included in the sample image 41 to be evaluated first, the color development pattern 22A has a slightly darker color change, and the color development pattern 22B has a lighter color change.
  • the evaluation result estimation means compares the coloring patterns 22A and 22B included in the image 41 of the evaluation target specimen with the coloring patterns 22A and 22B of the evaluation result information 43A and B, It is estimated that the color development patterns 22A and 22B of the sample to be evaluated are evaluated as “safe”. Then, the evaluation result image generation unit generates an evaluation result image corresponding to the evaluation result. Thereby, even when the color change of the color pattern image of the specimen to be evaluated does not completely match the color change of the color pattern image stored in the database 12, an evaluation result image can be generated. it can.
  • the first sample detection kit of the present embodiment includes a reagent for detecting a detection target in a sample with a color pattern and a sample detection device, and the sample detection device is the sample detection device of the present invention. It is characterized by.
  • the reagent is not particularly limited and includes the reagent described in the first embodiment.
  • the second sample detection kit of the present embodiment includes a reagent for detecting a detection target in a sample with a color pattern and a terminal, and the terminal is a terminal of the sample detection system of the present invention.
  • the reagent is not particularly limited and includes the reagent described in the first embodiment.
  • the program of this embodiment is a program that can execute the sample evaluation method of Embodiment 1 or 2 on a computer. Or the program of this embodiment may be recorded on a recording medium, for example.
  • the recording medium is not particularly limited, and examples thereof include a read only memory (ROM), a hard disk (HD), an optical disk, and a floppy (registered trademark) disk (FD).

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  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'objet de la présente invention est de pourvoir à un dispositif d'évaluation d'analyte, à un système d'évaluation d'analyte, à un procédé d'évaluation d'analyte, à un programme, et à un moyen d'enregistrement grâce auxquels même une personne qui ne dispose pas de connaissances d'expert peut comprendre sans ambiguïté les résultats de la détection dudit analyte par l'intermédiaire d'une réaction couleur. Pour atteindre cet objet, le dispositif de détection d'analyte (3) comprend : un moyen d'acquisition d'image de motif couleur (11) qui acquiert une image contenant le motif couleur d'un analyte ; un moyen de stockage de résultat de l'évaluation (12) qui stocke à l'avance, à titre d'information de résultat de l'évaluation, le motif couleur de l'analyte et un résultat de l'évaluation de l'analyte correspondant au motif couleur de l'analyte associés l'un à l'autre ; un moyen de génération d'image de résultat de l'évaluation (13) qui génère une image de résultat de l'évaluation correspondante à partir du motif couleur de l'image contenant le motif couleur par référence à l'information de résultat de l'évaluation stockée dans le moyen de stockage de résultat de l'évaluation (12) ; et un moyen de génération d'image composite (14) qui génère une image composite qui combine l'image contenant le motif couleur et l'image du résultat de l'évaluation.
PCT/JP2014/079083 2014-03-26 2014-10-31 Dispositif de détection d'analyte, système de détection d'analyte, kit de détection d'analyte, procédé de détection d'analyte, programme, et moyen d'enregistrement WO2015145851A1 (fr)

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Cited By (1)

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JP2021081428A (ja) * 2019-11-15 2021-05-27 台湾ナノカーボンテクノロジー股▲ふん▼有限公司Ｔａｉｗａｎ Ｃａｒｂｏｎ Ｎａｎｏ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｒｐｏｒａｔｉｏｎ 製品ラベル、生鮮食品の即時情報を提供するシステム及び方法

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JP2007212391A (ja) * 2006-02-13 2007-08-23 Toppan Forms Co Ltd 検体検査具
WO2010095394A1 (fr) * 2009-02-20 2010-08-26 寺本京史 Procédé de télédiagnostic
WO2011016565A1 (fr) * 2009-08-07 2011-02-10 Ｎｅｃソフト株式会社 Elément d'acide nucléique utilisable dans le cadre d'une analyse et procédé, réactif et instrument d'analyse l'utilisant
WO2012086599A1 (fr) * 2010-12-20 2012-06-28 Ｎｅｃソフト株式会社 Outil de détection et système de détection

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JP2006300810A (ja) * 2005-04-22 2006-11-02 Mitsubishi Electric Corp 鮮度識別装置及びそれを備えた冷蔵庫、鮮度識別方法
JP2007212391A (ja) * 2006-02-13 2007-08-23 Toppan Forms Co Ltd 検体検査具
WO2010095394A1 (fr) * 2009-02-20 2010-08-26 寺本京史 Procédé de télédiagnostic
WO2011016565A1 (fr) * 2009-08-07 2011-02-10 Ｎｅｃソフト株式会社 Elément d'acide nucléique utilisable dans le cadre d'une analyse et procédé, réactif et instrument d'analyse l'utilisant
WO2012086599A1 (fr) * 2010-12-20 2012-06-28 Ｎｅｃソフト株式会社 Outil de détection et système de détection

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021081428A (ja) * 2019-11-15 2021-05-27 台湾ナノカーボンテクノロジー股▲ふん▼有限公司Ｔａｉｗａｎ Ｃａｒｂｏｎ Ｎａｎｏ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｒｐｏｒａｔｉｏｎ 製品ラベル、生鮮食品の即時情報を提供するシステム及び方法
JP7090681B2 (ja) 2019-11-15 2022-06-24 台湾ナノカーボンテクノロジー股▲ふん▼有限公司 製品ラベル、生鮮食品の即時情報を提供するシステム及び方法

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