WO2015127365A3 - Mutants de sortase a calcium-indépendants - Google Patents
Mutants de sortase a calcium-indépendants Download PDFInfo
- Publication number
- WO2015127365A3 WO2015127365A3 PCT/US2015/017116 US2015017116W WO2015127365A3 WO 2015127365 A3 WO2015127365 A3 WO 2015127365A3 US 2015017116 W US2015017116 W US 2015017116W WO 2015127365 A3 WO2015127365 A3 WO 2015127365A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- residue
- sortase
- calcium
- amino acid
- mutants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/2207—Sortase A (3.4.22.70)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Cette invention concerne des mutants de sortase A calcium-indépendants manifestant une activité catalytique accrue comparativement à la sortase A de type sauvage.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201461943042P | 2014-02-21 | 2014-02-21 | |
US61/943,042 | 2014-02-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2015127365A2 WO2015127365A2 (fr) | 2015-08-27 |
WO2015127365A3 true WO2015127365A3 (fr) | 2015-10-15 |
Family
ID=53879254
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2015/017116 WO2015127365A2 (fr) | 2014-02-21 | 2015-02-23 | Mutants de sortase a calcium-indépendants |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2015127365A2 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018005716A2 (fr) * | 2016-07-01 | 2018-01-04 | Denali Therapeutics Inc. | Variants de l'albumine pour une demi-vie sérique améliorée |
EP4196590A4 (fr) * | 2021-01-28 | 2023-12-06 | Genequantum Healthcare (Suzhou) Co., Ltd. | Protéines de fusion de ligase et applications associées |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6773706B2 (en) * | 1999-04-15 | 2004-08-10 | The Regents Of The University Of California | Identification of sortase gene |
WO2013016653A1 (fr) * | 2011-07-28 | 2013-01-31 | Cell Signaling Technology, Inc. | Détection multi-composants |
-
2015
- 2015-02-23 WO PCT/US2015/017116 patent/WO2015127365A2/fr active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6773706B2 (en) * | 1999-04-15 | 2004-08-10 | The Regents Of The University Of California | Identification of sortase gene |
WO2013016653A1 (fr) * | 2011-07-28 | 2013-01-31 | Cell Signaling Technology, Inc. | Détection multi-composants |
Non-Patent Citations (4)
Title |
---|
DATABASE NCBI, Genbank 17 November 2009 (2009-11-17), "Staphylococcus aureus subsp. aureus ED98", XP055231327, Database accession no. ACY12423 * |
HIRAKAWA ET AL.: "Design of Ca2+-independent Staphylococcus aureus sortase A mutants", BIOTECHNOLOGY AND BIOENGINEERING, vol. 109, no. 12, 4 July 2012 (2012-07-04), pages 2955 - 2961, XP055210375 * |
LOWDER ET AL.: "Recent human-to-poultry host jump, adaptation, and pandemic spread of Staphylococcus aureus", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 106, no. 46, 17 November 2009 (2009-11-17), pages 19545 - 19550, XP055039038 * |
MARESSO ET AL.: "Sortase as a Target of Anti-Infective Therapy", PHARMACOLOGICAL REVIEWS, vol. 60, no. 1, 2008, pages 128 - 141, XP055231326 * |
Also Published As
Publication number | Publication date |
---|---|
WO2015127365A2 (fr) | 2015-08-27 |
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