WO2015118200A1 - System and method for analysing samples - Google Patents

System and method for analysing samples Download PDF

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Publication number
WO2015118200A1
WO2015118200A1 PCT/ES2015/070060 ES2015070060W WO2015118200A1 WO 2015118200 A1 WO2015118200 A1 WO 2015118200A1 ES 2015070060 W ES2015070060 W ES 2015070060W WO 2015118200 A1 WO2015118200 A1 WO 2015118200A1
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WO
WIPO (PCT)
Prior art keywords
platform
data
support
analysis
laser head
Prior art date
Application number
PCT/ES2015/070060
Other languages
Spanish (es)
French (fr)
Inventor
José Miguel AVELLÁ OLIVER
Sergi Beñat MORAIS EZQUERRO
Javier Carrascosa Rubio
Ángel MAQUIEIRA CATALA
Rosa Puchades Pla
Original Assignee
Universitat Politècnica De València
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Publication of WO2015118200A1 publication Critical patent/WO2015118200A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/024—Storing results with means integrated into the container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Definitions

  • the present invention relates to a device and a method that makes use of it in order to carry out data taking in analysis applications of different natures such as chemical or biological material analysis.
  • Analytical methods of samples of biological materials are known in the state of the art, such as systems and methods of testing and / or analysis directed at biological materials.
  • compact disk technologies are known in two large groups: data storage and disk labeling.
  • the first group also called optical disc recording technologies, is based on the use of compact discs as a means of storing and reading information.
  • optical disc recording technologies such as CD, DVD, SACD (Super audio CD), Blu-ray, etc.
  • the second group of technologies is focused on creating labels on disks belonging to the first group. They are based on the laser irradiation of photosensitive materials contained in the discs. These tag creation technologies, in their commercial form, are integrated into the devices and disks of the first group. Thus, there are commercial readers / recorders and discs compatible with these labeling technologies.
  • the present invention relates to a biological material analysis system that makes use of compact disc technology comprising at least one layer of thermosensitive, photosensitive, thermochromic or photochromic material, known among other names as LightScribe technology , in the field of analysis in applications that do not involve thermochromic material; that is to say, the disk is basically taken as a mere support after modifying it.
  • compact disc technology comprising at least one layer of thermosensitive, photosensitive, thermochromic or photochromic material, known among other names as LightScribe technology , in the field of analysis in applications that do not involve thermochromic material; that is to say, the disk is basically taken as a mere support after modifying it.
  • the supports can be covered by different layers of different materials, it is envisaged in this invention to obtain said analysis platforms with interesting materials by assembling the part of the support defined by the disk containing the coordinate code for the recognition and management of disk movement (control feature zone), with other platforms containing interesting materials for the analytical process.
  • the part of the disc that does not contain the central flat ring is replaced or modified in such a way that it comprises a sheet of polymeric material, preferably polystyrene, in said part of the disc that does not contain the central ring (a LightScribe CD for example ) with a coordinate code for the recognition and management of the movement of the laser head along the surface of the platform.
  • the system described here allows to carry out the analysis process in flat circular platforms of different diameters, as well as in non-circular platforms with different interesting ways for the development of analytical applications.
  • an analysis method is carried out using an analytical platform that supports LightScribe and DVD LightScribe CDs, DVD LightScribe discs without the thermochromic / photochromic coating layer and CD LightScribe discs without the thermochromic / photochromic coating layer .
  • the analytical process is also carried out using LightScribe CD discs in which the thermochromic / photochromic coating layer, the metal layer and the data recording dye layer have been removed as an analysis platform.
  • the analytical process is carried out on a 4-pointed blade-shaped platform.
  • This morphology improves the isolation of 4 different analysis zones.
  • This non-circular platform is obtained either by cutting the circular platforms, with circular support, described, or by assembling non-circular platforms, with other support geometries.
  • FIG. 1 Scheme of a possible configuration of a LightScribe disk reader and a LightScribe disk for use in analytical applications.
  • FIG. 2 Possible process of obtaining analysis platforms by assembling on the disk-defined support of the encoded element that contains the coordinate code for the recognition and management of the movement of the disk (control feature zone).
  • FIG. 3 Scheme of a test platform related to an application that involves the isolation of four areas of analysis, different materials and tests, and the recording of data related to the analysis in a portion of photochromic / thermochromic material contained in the disc.
  • the laser head (5) is responsible for emitting a radiation that passes through the analysis zone (6) and reaches a transmission analyzer (7) that receives the radiation once said analysis zone (6) passes and sends data referred to said radiation received to a process unit (8) which is responsible for carrying out the processes necessary to carry out an analysis of what is contained in the analysis area (6) from said data.
  • the label writing unit in photochromic coatings of optical data media writes the result of the analysis on a portion of the test platform (1) containing the photochromic / thermochromic material of the discs labeled by laser radiation.
  • a radiation source defined by the laser head (5) is used together with a reflection analyzer, the analysis platform (1) which in turn comprises the support (2 ) and the encoded element (3) fixed on the support (2) which comprises data referring to: a movement of the laser head (5) and the operation of a motor (4) of the reading and / or writing data unit in optical means intended to generate the rotation of the platform (1), a code reading laser head (5 ') or analyzer of the encoded element, a platform movement system, an analysis or test in the analysis area ( 6), the transmission analyzer (7) and the process unit (8).
  • the analysis platform (1) made of a suitable material, is configured by assembling a disk of the part of the support (2) of the encoded element (3) containing the coordinate code for the recognition and management of the movement of the platform (1).
  • Figure 2 shows a possible configuration process based on the extraction of the original encoded element from an original optical data disk, followed by the assembly of the encoded element (3), which comprises at least one sheet of a material other than that of the disk or comprising the said embedded coding, in order to create the platform (1) comprising the disk, ie the support (2), and the encoded element (3).
  • Microarray format assays are one of the analytical procedures with the greatest potential for the development of analytical applications based on the system object of the invention. Microarray format assays are based on the immobilization of specific probes (proteins, nucleic acids, carbohydrates, microorganisms, molecular imprinting materials, etc.) in certain positions of the platforms of the sample analysis system described herein.
  • bioreceptors labeled with colloidal gold particles and silver salts in solution are used for signal generation in the microarray assays described above.
  • bioreceptors labeled with horseradish peroxidase and 3,3 ', 5,5'-Tetramethylbenzine are used in solution for signal generation in assays
  • antibodies are immobilized on the platforms to carry out an immunoassay in microarray format for the detection of proteins, antibodies, organic compounds and cells.
  • protein-hapten conjugates are immobilized on the described platforms to carry out analyzes of molecular organic compounds.
  • nucleic acids are immobilized on the platforms described to carry out a DNA hybridization assay in a microarray format for the detection of pathogenic organisms.
  • a cell culture medium is dispensed on these platforms to carry out microbiological analyzes, such as colony forming units counting or ecotoxicity assays.
  • immunofiltration membranes are fixed on the described platforms, and using laser radiation, labelable systems capable of carrying out immunoconcentrations and immunofiltration tests are created.
  • vinylidene polyfluoride membranes are fixed on the platform, bioreceptors are immobilized on the membrane, the sample is filtered, bioreceptors labeled with colloidal gold are filtered, and finally a silver solution is filtered to develop the signal.
  • the platforms (1) comprise optically active optical / photon structures interesting for the analysis or data collection stage.
  • optically active gratings are created on the platforms for carrying out non-malignant detection of molecular biorecognition events involved in the analytical process.
  • platforms (1) can be obtained for a wide range of classical analytical methods, such as those related to molecular absorption, turbidimetry and nephelometry.
  • the platforms (1) can have: • adhesive polymeric chambers on the described platforms, and for example the concentration of chloride ion in an aqueous sample is determined by measuring the turbidimetric signal of silver chloride.
  • only one area of the platform (1) that contains a data collection area is selectively irradiated, thus minimizing the volume occupied by the analytical records.
  • FIG. 3 which involves the isolation of 4 analysis zones (6), different materials and tests, and the recording of data relating to the analysis in a portion of photochromic / thermochromic material contained in the disk.
  • Use is made of an analysis area, an analysis or test, data relating to the test recorded on the thermochromic / photochromic material, thermochromic / photochromic material, and the encoded element fixed on the support.
  • the encoded element (3) can be located in a central part of the support (2) and with flat annular shape of internal diameter coinciding with that of an optical media fixing element of the reading and / or writing unit in optical media; also said encoded element (3) is the one that carries the data referred to at least one movement to be performed by the laser head (5.5 ') with respect to the platform.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a system for analysing samples and a method for analysing samples using said system, based on using units for reading/writing data on optical media with the ability to label said optical discs, such as those known as "LightScribe". For this purpose, the optical media comprise a carrier for data which can be read by the read unit via its head, data comprising the necessary information relating to the movements and actions of the motor of the unit and the operation of the laser head, thereby defining the read/write operation on the medium.

Description

SISTEMA Y MÉTODO DE ANÁLISIS DE MUESTRAS  SAMPLE ANALYSIS SYSTEM AND METHOD
DESCRIPCIÓN La presente invención se refiere a un dispositivo y un método que hace uso del mismo para poder llevar a cabo tomas de datos en aplicaciones de análisis de distintas naturalezas como puede ser análisis químico o de material biológico. DESCRIPTION The present invention relates to a device and a method that makes use of it in order to carry out data taking in analysis applications of different natures such as chemical or biological material analysis.
ESTADO DE LA TÉCNICA STATE OF THE TECHNIQUE
Son conocidos en el estado de la técnica los métodos analíticos de muestras de materiales biológicos como puede ser los sistemas y método de ensayo y/o análisis dirigidos a materiales biológicos. Igualmente son conocidas las tecnologías de disco compacto en dos grandes grupos: las de almacenamiento de datos y las de etiquetaje de los discos. Analytical methods of samples of biological materials are known in the state of the art, such as systems and methods of testing and / or analysis directed at biological materials. Likewise, compact disk technologies are known in two large groups: data storage and disk labeling.
El primer grupo, también llamado optical disc recording technologies, está basado en el uso de discos compactos como medio de almacenamiento y lectura de información. Existen varios sistemas comerciales pertenecientes a optical disc recording technologies, como son CD, DVD, SACD (Super audio CD), Blu-ray, etc. The first group, also called optical disc recording technologies, is based on the use of compact discs as a means of storing and reading information. There are several commercial systems belonging to optical disc recording technologies, such as CD, DVD, SACD (Super audio CD), Blu-ray, etc.
El segundo grupo de tecnologías está enfocado en la creación de etiquetas en discos pertenecientes al primer grupo. Se basan en la irradiación láser de materiales fotosensibles contenidos en los discos. Estas tecnologías de creación de etiquetas, en su forma comercial, se encuentran integradas en los dispositivos y discos del primer grupo. Así pues, existen lectores/grabadores y discos comerciales compatibles con estas tecnologías de etiquetaje. The second group of technologies is focused on creating labels on disks belonging to the first group. They are based on the laser irradiation of photosensitive materials contained in the discs. These tag creation technologies, in their commercial form, are integrated into the devices and disks of the first group. Thus, there are commercial readers / recorders and discs compatible with these labeling technologies.
Actualmente existen tres sistemas comerciales pertenecientes a este segundo grupo: DiscT@2, Labelflash y LightScribe. Los desarrollos descritos en la propuesta de patente están todos llevados a cabo usando la tecnología LightScribe. There are currently three commercial systems belonging to this second group: DiscT @ 2, Labelflash and LightScribe. The developments described in the Patent proposals are all carried out using LightScribe technology.
DESCRIPCIÓN DE LA INVENCIÓN DESCRIPTION OF THE INVENTION
En un primer aspecto, la presente invención se refiere a un sistema de análisis de material biológico que hace uso de la tecnología de discos compactos que comprenden al menos una capa de material termosensible, fotosensible, termocrómico o fotocrómico, conocidos entre otros nombres como tecnología LightScribe, en el campo del análisis en aplicaciones que no involucran el material termocrómico; es decir el disco se toma básicamente como un mero soporte previa modificación del mismo. In a first aspect, the present invention relates to a biological material analysis system that makes use of compact disc technology comprising at least one layer of thermosensitive, photosensitive, thermochromic or photochromic material, known among other names as LightScribe technology , in the field of analysis in applications that do not involve thermochromic material; that is to say, the disk is basically taken as a mere support after modifying it.
El potencial de la tecnología de disco en el campo del análisis reside tanto en el uso de los lectores de disco a modo de instrumentación analítica como en el empleo los discos como plataforma de análisis. A partir de los discos etiquetables mediante radiación láser comerciales se pueden obtener diferentes plataformas interesantes. De esta manera y dada la ausencia de limitaciones ópticas de los sistemas etiquetables mediante radiación láser se pueden generar distintas plataformas de análisis con soportes como los discos recubiertos por materiales interesantes para el proceso analítico (polímeros, metales, óxidos de metales, silicio, óxido de silicio, geles, etc). The potential of disk technology in the field of analysis lies both in the use of disk readers as an analytical instrumentation and in the use of disks as an analysis platform. Different interesting platforms can be obtained from the labels that can be labeled using commercial laser radiation. In this way and given the absence of optical limitations of the systems that can be labeled by means of laser radiation, different analysis platforms can be generated with supports such as discs coated with interesting materials for the analytical process (polymers, metals, metal oxides, silicon, oxide of silicon, gels, etc).
Estas plataformas se pueden obtener mediante el recubrimiento, con estos materiales citados anteriormente, como puede ser discos o soportes recubiertos por una capa de oro depositada por la técnica de sputtering o por una capa de poliestireno depositada por la técnica de spin coating. These platforms can be obtained by coating, with these materials mentioned above, such as discs or supports coated by a layer of gold deposited by the sputtering technique or by a layer of polystyrene deposited by the spin coating technique.
Dado que los soportes pueden ser recubiertos por distintas capas de distintos materiales, se prevé en esta invención la obtención de dichas plataformas de análisis con materiales interesantes mediante el ensamblado de la parte del soporte definido por el disco que contiene el código de coordenadas para el reconocimiento y gestión del movimiento del disco (control feature zone), con otras plataformas conteniendo materiales interesantes para el proceso analítico. Para ello, la parte del disco que no contiene el anillo plano central es sustituida o modificada de tal manera que ésta comprenda una lámina de material polimérico, preferiblemente poliestireno, en dicha parte del disco que no contenga el anillo central (un CD LightScribe por ejemplo) con un código de coordenadas para el reconocimiento y la gestión del movimiento del cabezal láser a lo largo de la superficie de la plataforma. Since the supports can be covered by different layers of different materials, it is envisaged in this invention to obtain said analysis platforms with interesting materials by assembling the part of the support defined by the disk containing the coordinate code for the recognition and management of disk movement (control feature zone), with other platforms containing interesting materials for the analytical process. For this, the part of the disc that does not contain the central flat ring is replaced or modified in such a way that it comprises a sheet of polymeric material, preferably polystyrene, in said part of the disc that does not contain the central ring (a LightScribe CD for example ) with a coordinate code for the recognition and management of the movement of the laser head along the surface of the platform.
El sistema aquí descrito permite llevar a cabo el proceso de análisis en plataformas circulares planas de distintos diámetros, así como en plataformas no circulares con diferentes formas interesantes para el desarrollo de aplicaciones analíticas.  The system described here allows to carry out the analysis process in flat circular platforms of different diameters, as well as in non-circular platforms with different interesting ways for the development of analytical applications.
En una realización preferida se lleva a cabo un método de análisis usando una plataforma analítica que presenta como soporte discos CD LightScribe y DVD LightScribe, discos DVD LightScribe sin la capa de recubrimiento termocrómico/fotocrómico y discos CD LightScribe sin la capa de recubrimiento termocrómico/fotocrómico. En esta realización preferente se lleva también a cabo el proceso analítico usando como plataforma de análisis discos CD LightScribe en los que se le han eliminado la capa de recubrimiento termocrómico/fotocrómico, la capa metálica y la capa de tinte de grabación de datos. In a preferred embodiment, an analysis method is carried out using an analytical platform that supports LightScribe and DVD LightScribe CDs, DVD LightScribe discs without the thermochromic / photochromic coating layer and CD LightScribe discs without the thermochromic / photochromic coating layer . In this preferred embodiment, the analytical process is also carried out using LightScribe CD discs in which the thermochromic / photochromic coating layer, the metal layer and the data recording dye layer have been removed as an analysis platform.
En una realización más preferida se lleva a cabo el proceso analítico en una plataforma en forma de aspa de 4 puntas. Esta morfología mejora el aislamiento de 4 zonas de análisis diferentes. Esta plataforma no circular es obtenida o bien mediante recorte de las plataformas circulares, con soporte circular, descritas, o bien ensamblando plataformas no circulares, con otras geometrías de soporte. BREVE DESCRIPCIÓN DE LAS FIGURAS In a more preferred embodiment, the analytical process is carried out on a 4-pointed blade-shaped platform. This morphology improves the isolation of 4 different analysis zones. This non-circular platform is obtained either by cutting the circular platforms, with circular support, described, or by assembling non-circular platforms, with other support geometries. BRIEF DESCRIPTION OF THE FIGURES
Para complementar la descripción que se está realizando y con objeto de ayudar a una mejor comprensión de las características de la invención, de acuerdo con un ejemplo preferente de realización práctica de la misma, se acompaña como parte integrante de dicha descripción, un juego de dibujos en donde con carácter ilustrativo y no limitativo, se ha representado lo siguiente: FIG. 1. Esquema de una posible configuración del un lector de discos LightScribe y un disco LightScribe para su uso en aplicaciones analíticas. To complement the description that is being made and in order to help a better understanding of the characteristics of the invention, according to a preferred example of practical implementation thereof, a set of drawings is attached as an integral part of said description. where, for illustrative and non-limiting purposes, the following has been represented: FIG. 1. Scheme of a possible configuration of a LightScribe disk reader and a LightScribe disk for use in analytical applications.
FIG. 2. Posible proceso de obtención de plataformas de análisis mediante el ensamblado en el soporte definido por el disco del elemento codificado que contiene el código de coordenadas para el reconocimiento y gestión del movimiento del disco (control feature zone). FIG. 2. Possible process of obtaining analysis platforms by assembling on the disk-defined support of the encoded element that contains the coordinate code for the recognition and management of the movement of the disk (control feature zone).
FIG. 3. Esquema de una plataforma de ensayo relativa a una aplicación que involucra el aislamiento de cuatro zonas de análisis, diferentes materiales y ensayos, y el grabado de datos referentes al análisis en una porción de material fotocrómico/termocrómico contenida en el disco. FIG. 3. Scheme of a test platform related to an application that involves the isolation of four areas of analysis, different materials and tests, and the recording of data related to the analysis in a portion of photochromic / thermochromic material contained in the disc.
REALIZACIÓN PREFERENTE DE LA INVENCIÓN A continuación se ¡lustrará la invención mediante unos ensayos realizados por los inventores, que pone de manifiesto la efectividad del producto de la invención. Tanto el sistema como el método aquí descritos hacen uso de un algoritmo informático que se encarga de determinar automáticamente un resultado analítico a partir de la información obtenida de la lectura de una zona de análisis (6) de una plataforma (1 ) analítica del sistema objeto de la invención, plataforma (1 ) que comprende un soporte (2) y un elemento codificado (3) fijado en el soporte (2), que a su vez comprende datos referidos a operaciones a llevar a cabo por al menos un cabezal láser (5,5') ya sea éste un movimiento de un cabezal láser (5) o movimientos referidos a un cabezal láser de lectura de códigos (5'), en cualquier caso el cabezal láser (5,5') dispone de capacidad de lectura y/o escritura de datos y puede ser el de una unidad de lectura y/o escritura de datos en medios ópticos de datos que presenta un único cabezal láser (5,5') que se encarga de operar sobre la plataforma, o un cabezal láser de lectura de códigos (5') adicional dedicado. El cabezal láser (5) se encarga de emitir una radiación que pasa por la zona de análisis (6) y llega a un analizador de transmisión (7) que recibe la radiación una vez pasa dicha zona de análisis (6) y hace llegar datos referidos a dicha radiación recibida a una unidad de proceso (8) la cual se encarga de llevar a cabo los procesos necesarios para llevar a cabo un análisis de aquello contenido en la zona de análisis (6) a partir de dichos datos. Posteriormente, la unidad de escritura de etiquetas en recubrimientos fotocrómicos de medios ópticos de datos, escribe el resultado del análisis en una porción de la plataforma (1 ) de ensayo que contiene el material fotocrómico/termocrómico de los discos etiquetables mediante radiación láser. Esta aplicación permite un acceso rápido y directo por parte del usuario a la información obtenida del análisis, hecho que facilita el proceso de análisis a los usuarios no especializados. Tal y como se observa en la figura 1 en esta realización se emplea: una fuente de radiación definida por el cabezal láser (5) junto con un analizador de reflexión, la plataforma (1 ) de análisis que a su vez comprende el soporte (2) y el elemento codificado (3) fijado en el soporte (2) el cual comprende datos referidos a: un movimiento del cabezal láser (5) y al funcionamiento un motor (4) de la unidad de lectura y/o escritura de datos en medios ópticos destinado a generar la rotación de la plataforma (1 ), el un cabezal láser de lectura de códigos (5') o analizador del elemento codificado, un sistema de movimiento de la plataforma, un análisis o ensayo en la zona de análisis (6), el analizador de transmisión (7) y la unidad de proceso (8). La plataforma (1 ) de análisis, fabricada de un material adecuado, se configura mediante ensamblado a un disco de la parte del soporte (2) del elemento codificado (3) que contiene el código de coordenadas para el reconocimiento y gestión del movimiento de la plataforma (1 ). En la figura 2 se muestra un posible proceso de configuración basado en la extracción del elemento codificado original de un disco óptico de datos original, seguido del ensamblado del el elemento codificado (3), el cual comprende al menos una lámina de un material diferente a aquel del disco o que comprende la citada codificación embebida, para crear así la plataforma (1 ) que comprende el disco, es decir el soporte (2), y el elemento codificado (3). PREFERRED EMBODIMENT OF THE INVENTION The invention will now be illustrated by tests carried out by the inventors, which demonstrates the effectiveness of the product of the invention. Both the system and the method described here make use of a computer algorithm that is responsible for automatically determining an analytical result from the information obtained from reading an analysis area (6) of an analytical platform (1) of the object system of the invention, platform (1) comprising a support (2) and an encoded element (3) fixed on the support (2), which in turn comprises referred data to operations to be carried out by at least one laser head (5.5 ') whether this is a movement of a laser head (5) or movements referred to a laser head for code reading (5'), in any case the laser head (5.5 ') has the ability to read and / or write data and can be that of a unit for reading and / or writing data in optical data media that has a single laser head (5.5' ) that is responsible for operating on the platform, or a dedicated additional code reading laser head (5 '). The laser head (5) is responsible for emitting a radiation that passes through the analysis zone (6) and reaches a transmission analyzer (7) that receives the radiation once said analysis zone (6) passes and sends data referred to said radiation received to a process unit (8) which is responsible for carrying out the processes necessary to carry out an analysis of what is contained in the analysis area (6) from said data. Subsequently, the label writing unit in photochromic coatings of optical data media writes the result of the analysis on a portion of the test platform (1) containing the photochromic / thermochromic material of the discs labeled by laser radiation. This application allows quick and direct access by the user to the information obtained from the analysis, which facilitates the analysis process to non-specialized users. As can be seen in Figure 1 in this embodiment, a radiation source defined by the laser head (5) is used together with a reflection analyzer, the analysis platform (1) which in turn comprises the support (2 ) and the encoded element (3) fixed on the support (2) which comprises data referring to: a movement of the laser head (5) and the operation of a motor (4) of the reading and / or writing data unit in optical means intended to generate the rotation of the platform (1), a code reading laser head (5 ') or analyzer of the encoded element, a platform movement system, an analysis or test in the analysis area ( 6), the transmission analyzer (7) and the process unit (8). The analysis platform (1), made of a suitable material, is configured by assembling a disk of the part of the support (2) of the encoded element (3) containing the coordinate code for the recognition and management of the movement of the platform (1). Figure 2 shows a possible configuration process based on the extraction of the original encoded element from an original optical data disk, followed by the assembly of the encoded element (3), which comprises at least one sheet of a material other than that of the disk or comprising the said embedded coding, in order to create the platform (1) comprising the disk, ie the support (2), and the encoded element (3).
Los ensayos en formato de micromatriz son uno de los procedimientos analíticos con mayor potencial para el desarrollo de aplicaciones analíticas basadas en el sistema objeto de la invención. Los ensayos en formato de micromatriz se basan en la inmovilización de sondas específicas (proteínas, ácidos nucleicos, carbohidratos, microorganismos, materiales de impronta molecular, etc.) en determinadas posiciones de las plataformas del sistema de análisis de muestras aquí descrito. En una realización preferida se usan bioreceptores marcados con partículas de oro coloidal y sales de plata en disolución para la generación de la señal en los ensayos de micromatriz descritos anteriormente. En otro aspecto de esta realización se usan bioreceptores marcados con peroxidasa de rábano picante y 3,3',5,5'-Tetrametilbenz¡d¡na en disolución para la generación de señal en ensayos Microarray format assays are one of the analytical procedures with the greatest potential for the development of analytical applications based on the system object of the invention. Microarray format assays are based on the immobilization of specific probes (proteins, nucleic acids, carbohydrates, microorganisms, molecular imprinting materials, etc.) in certain positions of the platforms of the sample analysis system described herein. In a preferred embodiment, bioreceptors labeled with colloidal gold particles and silver salts in solution are used for signal generation in the microarray assays described above. In another aspect of this embodiment, bioreceptors labeled with horseradish peroxidase and 3,3 ', 5,5'-Tetramethylbenzine are used in solution for signal generation in assays
En una realización preferida del método aquí descrito se inmovilizan anticuerpos sobre las plataformas para llevar cabo un inmunoensayo en formato de micromatriz para la detección de proteínas, anticuerpos, compuestos orgánicos y células. En una realización más preferida, se inmovilizan conjugados proteína-hapteno sobre las plataforma descritas para llevar a cabo análisis de compuestos orgánicos moleculares. En una realización más prefenda, se inmovilizan ácidos nucleicos sobre las plataformas descritas para llevar cabo un ensayo de hibridación de ADN en formato de micromatriz para la detección organismos patógenos. In a preferred embodiment of the method described herein, antibodies are immobilized on the platforms to carry out an immunoassay in microarray format for the detection of proteins, antibodies, organic compounds and cells. In a more preferred embodiment, protein-hapten conjugates are immobilized on the described platforms to carry out analyzes of molecular organic compounds. In a more preferred embodiment, nucleic acids are immobilized on the platforms described to carry out a DNA hybridization assay in a microarray format for the detection of pathogenic organisms.
En una realización aún más preferida se dispensa un medio de cultivo celular sobre estas plataformas para llevar a cabo análisis microbiológicos, tales como recuento de unidades formadoras de colonias o ensayos de ecotoxicidad. In an even more preferred embodiment, a cell culture medium is dispensed on these platforms to carry out microbiological analyzes, such as colony forming units counting or ecotoxicity assays.
En una realización todavía más preferida se fijan membranas de inmunofiltración sobre las plataformas descritas, y utilizando radiación láser se crean sistemas etiquetables capaces de llevar a cabo inmuno- preconcentraciones y ensayos de inmunofiltración. En una realización preferente se fijan membranas de polifluoruro de vinilideno sobre la plataforma, se inmovilizan bioreceptores en la membrana, se filtra la muestra, se filtran bioreceptores marcados con oro coloidal, y finalmente se filtra una disolución de plata para desarrollar la señal. In an even more preferred embodiment, immunofiltration membranes are fixed on the described platforms, and using laser radiation, labelable systems capable of carrying out immunoconcentrations and immunofiltration tests are created. In a preferred embodiment, vinylidene polyfluoride membranes are fixed on the platform, bioreceptors are immobilized on the membrane, the sample is filtered, bioreceptors labeled with colloidal gold are filtered, and finally a silver solution is filtered to develop the signal.
En una realización aún más preferida las plataformas (1 ) comprenden estructuras óptica/fotón icam ente activas interesantes para la etapa de análisis o toma de datos. En una realización preferente se crean gratings ópticamente activos sobre las plataformas para llevar a cabo detección sin mareaje de eventos de bioreconocimiento molecular involucrados en el proceso analítico. In an even more preferred embodiment, the platforms (1) comprise optically active optical / photon structures interesting for the analysis or data collection stage. In a preferred embodiment, optically active gratings are created on the platforms for carrying out non-malignant detection of molecular biorecognition events involved in the analytical process.
En otro aspecto del método aquí descrito se pueden obtener plataformas (1 ) para un gran abanico de métodos analíticos clásicos, como los relacionados con la absorción molecular, la turbidimetría y la nefelometría. In another aspect of the method described herein, platforms (1) can be obtained for a wide range of classical analytical methods, such as those related to molecular absorption, turbidimetry and nephelometry.
Dada la versatilidad de los ensayos, las plataformas (1 ) pueden disponer de: • cámaras poliméricas adhesivas sobre las plataformas descritas, y se determina por ejemplo la concentración de ión cloruro en una muestra acuosa a través de la medida de la señal turbidimétrica del cloruro de plata. Given the versatility of the tests, the platforms (1) can have: • adhesive polymeric chambers on the described platforms, and for example the concentration of chloride ion in an aqueous sample is determined by measuring the turbidimetric signal of silver chloride.
• cámaras que pueden ser adhesivas, y obtenidas mediante perforación transversal de las plataformas y el sellado de ambos orificios con una película polimérica adhesiva.  • chambers that can be adhesive, and obtained by cross-drilling the platforms and sealing both holes with an adhesive polymeric film.
• estructuras óptica/fotónicamente activas.  • optical / photonically active structures.
• uno o más elementos fluídicos integrados que permiten llevar a cabo el tratamiento de muestra y las diferentes etapas del ensayo, automatizando de esta forma el proceso analítico,  • one or more integrated fluidic elements that allow the sample treatment and the different stages of the test to be carried out, thus automating the analytical process,
• sistemas fluídicos centrífugos que consiguen plataformas de ensayo en las que tanto la etapa de lectura como la de bombeo de los fluidos se llevan a cabo con el lector de discos ópticos de datos.  • centrifugal fluid systems that achieve test platforms in which both the reading stage and the pumping stage of the fluids are carried out with the optical data disc reader.
• canales, válvulas, cámaras y demás dispositivos fluídicos en las plataformas descritas para automatizar un immunoensayo en formato de micromatriz.  • channels, valves, chambers and other fluidic devices on the platforms described to automate an immunoassay in microarray format.
En una realización aún más preferida se irradia selectivamente tan solo un área de la plataforma (1 ) que contiene una zona de toma de datos, minimizando así el volumen ocupado por los registros analíticos. In an even more preferred embodiment, only one area of the platform (1) that contains a data collection area is selectively irradiated, thus minimizing the volume occupied by the analytical records.
En una posible realización mostrada en la figura 3 que involucra el aislamiento de 4 zonas de análisis (6) , diferentes materiales y ensayos, y el grabado de datos referentes al análisis en una porción de material fotocrómico/termocrómico contenida en el disco. Se hace uso de una zona de análisis, un análisis o ensayo, datos referentes al ensayo grabados sobre el material termocrómico/fotocrómico, material termocrómico/fotocrómico, y el elemento codificado fijado en el soporte. In a possible embodiment shown in Figure 3 which involves the isolation of 4 analysis zones (6), different materials and tests, and the recording of data relating to the analysis in a portion of photochromic / thermochromic material contained in the disk. Use is made of an analysis area, an analysis or test, data relating to the test recorded on the thermochromic / photochromic material, thermochromic / photochromic material, and the encoded element fixed on the support.
En cualquiera de las posibles realizaciones descritas se puede tener el elemento codificado (3) ubicado en una parte central del soporte (2) y con forma anular plana de diámetro interior coincidente con aquel de un elemento de fijación de medios ópticos de la unidad de lectura y/o escritura en medios ópticos; asimismo dicho elemento codificado (3) es aquel que porta los datos referidos a al menos un movimiento a realizar por el cabezal láser (5,5') con respecto de la plataforma. In any of the possible embodiments described, the encoded element (3) can be located in a central part of the support (2) and with flat annular shape of internal diameter coinciding with that of an optical media fixing element of the reading and / or writing unit in optical media; also said encoded element (3) is the one that carries the data referred to at least one movement to be performed by the laser head (5.5 ') with respect to the platform.

Claims

REIVINDICACIONES
1 . Sistema de análisis que comprende una unidad de lectura y/o escritura de datos en medios ópticos de datos, que a su vez comprende un cabezal láser (5,5') con al menos capacidad de lectura/escritura de datos, one . Analysis system comprising a unit for reading and / or writing data in optical data media, which in turn comprises a laser head (5.5 ') with at least the ability to read / write data,
sistema caracterizado porque comprende al menos una plataforma (1 ) de análisis que a su vez comprende un soporte (2) y un elemento codificado (3) fijado en el soporte el cual comprende datos referidos al menos a: un movimiento del cabezal láser (5,5') y al funcionamiento de al menos uno de: cabezal (5,5') y motor (4) de la unidad de lectura y/o escritura de datos en medios ópticos destinado a generar la rotación de la plataforma (1 ).  system characterized in that it comprises at least one analysis platform (1) which in turn comprises a support (2) and an encoded element (3) fixed on the support which comprises data referring at least to: a movement of the laser head (5 , 5 ') and the operation of at least one of: head (5.5') and motor (4) of the unit for reading and / or writing data in optical media intended to generate the rotation of the platform (1) .
2. Sistema según la reivindicación 1 , caracterizado porque la plataforma (1 ) comprende adicionalmente al menos una capa de un matenal que recubre al menos parcialmente el soporte (2). 2. System according to claim 1, characterized in that the platform (1) additionally comprises at least one layer of a mat that covers at least partially the support (2).
3. Sistema según la reivindicación 1 , caracterizado porque el elemento codificado (3) comprende datos legibles por el cabezal láser (5,5'). 3. System according to claim 1, characterized in that the encoded element (3) comprises data readable by the laser head (5.5 ').
4. Sistema según una cualquiera de las reivindicaciones 1 a 3, caracterizado porque el elemento codificado (3) comprende datos referidos a al menos un movimiento a realizar por el cabezal láser (5,5') con respecto de la plataforma (1 ). System according to any one of claims 1 to 3, characterized in that the encoded element (3) comprises data referring to at least one movement to be performed by the laser head (5.5 ') with respect to the platform (1).
5. Sistema según una cualquiera de las reivindicaciones 2 a 4, caracterizado porque la capa de matenal comprende al menos un material que se selecciona de entre el grupo consistente en: polímeros, metales, óxidos de metales, silicio, óxido de silicio, y geles. 5. System according to any one of claims 2 to 4, characterized in that the matenal layer comprises at least one material that is selected from the group consisting of: polymers, metals, metal oxides, silicon, silicon oxide, and gels .
6. Sistema según una cualquiera de las reivindicaciones 1 a 5, caracterizado porque el soporte (2) tiene forma circular plana. System according to any one of claims 1 to 5, characterized in that the support (2) has a flat circular shape.
7. Sistema según reivindicación 1 ó 6, caracterizado porque el elemento codificado (3) se encuentra ubicado en una parte central del soporte (2) y tiene forma anular plana de diámetro interior coincidente con aquel de un elemento de fijación de medios ópticos de la unidad de lectura y/o escritura en medios ópticos. 7. System according to claim 1 or 6, characterized in that the encoded element (3) is located in a central part of the support (2) and has a flat annular shape of internal diameter coinciding with that of an optical means fixing element of the reading and / or writing unit in optical media.
8. Sistema según reivindicación 1 ó 6, caracterizado porque la plataforma (1 ) adicionalmente comprende al menos uno de los siguientes: System according to claim 1 or 6, characterized in that the platform (1) additionally comprises at least one of the following:
• al menos un elemento fluídico que se selecciona de entre: canal, válvula, cámaras, estructura fluídica y dispositivo fluídico, • at least one fluidic element that is selected from: channel, valve, chambers, fluidic structure and fluidic device,
• al menos una membrana de inmunofiltración, • at least one immunofiltration membrane,
• al menos una estructura óptica/fotón icam ente activas, y • at least one optically active photon / photon structure, and
• al menos una cámara polimérica adhesiva. • at least one polymeric adhesive chamber.
PCT/ES2015/070060 2014-02-05 2015-01-29 System and method for analysing samples WO2015118200A1 (en)

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