WO2015108869A3 - Compartiment cardiaque in vitro - Google Patents

Compartiment cardiaque in vitro Download PDF

Info

Publication number
WO2015108869A3
WO2015108869A3 PCT/US2015/011215 US2015011215W WO2015108869A3 WO 2015108869 A3 WO2015108869 A3 WO 2015108869A3 US 2015011215 W US2015011215 W US 2015011215W WO 2015108869 A3 WO2015108869 A3 WO 2015108869A3
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
pathology
stimuli
vitro
cells
Prior art date
Application number
PCT/US2015/011215
Other languages
English (en)
Other versions
WO2015108869A2 (fr
Inventor
Dov SHAMIR
Original Assignee
New York University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New York University filed Critical New York University
Publication of WO2015108869A2 publication Critical patent/WO2015108869A2/fr
Publication of WO2015108869A3 publication Critical patent/WO2015108869A3/fr
Priority to US15/210,636 priority Critical patent/US20170009207A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2529/00—Culture process characterised by the use of electromagnetic stimulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Physiology (AREA)
  • Computer Hardware Design (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Compartiment in vitro servant à simuler les forces mécaniques et électriques rencontrées in vivo. Un compartiment fournit des stimuli conditionnels. Les stimuli conditionnels simulent une pathologie, et peuvent simuler une pathologie spécifique. Le compartiment est configuré pour permettre des stimulis additionnels afin de tester l'effet de stimuli physiques, chimiques ou électriques sur des cellules présentant la pathologie spécifique. Des produits pharmaceutiques peuvent être testé ex vivo sur des cellules présentant une pathologie dans un environnement simulant l'environnement in vitro. Des composants physiques tels que des stimulateurs cardiaques peuvent également être testés sur de telles cellules dans ledit compartiment.
PCT/US2015/011215 2014-01-14 2015-01-13 Compartiment cardiaque in vitro WO2015108869A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US15/210,636 US20170009207A1 (en) 2014-01-14 2016-07-14 In-vitro bioreactor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461927356P 2014-01-14 2014-01-14
US61/927,356 2014-01-14

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US15/210,636 Continuation-In-Part US20170009207A1 (en) 2014-01-14 2016-07-14 In-vitro bioreactor

Publications (2)

Publication Number Publication Date
WO2015108869A2 WO2015108869A2 (fr) 2015-07-23
WO2015108869A3 true WO2015108869A3 (fr) 2015-09-11

Family

ID=53543603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/011215 WO2015108869A2 (fr) 2014-01-14 2015-01-13 Compartiment cardiaque in vitro

Country Status (2)

Country Link
US (1) US20170009207A1 (fr)
WO (1) WO2015108869A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6793918B2 (ja) * 2016-09-02 2020-12-02 日本光電工業株式会社 測定装置
EP3707235B1 (fr) * 2017-11-06 2022-07-06 Fondazione Istituto Firc Di Oncologia Molecolare (IFOM) Dispositif de compression cellulaire
EP3760702A1 (fr) 2019-07-04 2021-01-06 Celvivo ApS Bioréacteur et système de bioréacteur pour la croissance cellulaire et tissulaire
GB2589083A (en) * 2019-11-11 2021-05-26 Imperial College Innovations Ltd An apparatus & method
WO2022256768A1 (fr) * 2021-05-30 2022-12-08 University Of Florida Research Foundation, Inc. Dispositifs, systèmes et procédés relatifs aux bioréacteurs à perfusion pressurisés
EP4324908A1 (fr) * 2022-08-17 2024-02-21 International Centre For Genetic Engineering And Biotechnology - ICGEB Effecteur d'extrémité pour la stimulation volumétrique cellulaire
WO2024141677A1 (fr) 2022-12-28 2024-07-04 Leartiker S.Coop. Chambre de bioréacteur pour la croissance et/ou la maturation d'un tissu et procédé de croissance et/ou de maturation d'un tissu dans une chambre de bioréacteur

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4974461A (en) * 1988-10-13 1990-12-04 The United States Of America As Represented By Department Of Health And Human Services Anthropomorphic cardiac ultrasound phantom
US20080298540A1 (en) * 2007-05-29 2008-12-04 Mcgill University Deformable phantom apparatus
US20120034695A1 (en) * 2010-06-30 2012-02-09 Palaniappan Sethu Tissue/cell culturing system and related methods
US20130330701A1 (en) * 2012-06-12 2013-12-12 Vladimir Rubinstein Physical heart simulator

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4974461A (en) * 1988-10-13 1990-12-04 The United States Of America As Represented By Department Of Health And Human Services Anthropomorphic cardiac ultrasound phantom
US20080298540A1 (en) * 2007-05-29 2008-12-04 Mcgill University Deformable phantom apparatus
US20120034695A1 (en) * 2010-06-30 2012-02-09 Palaniappan Sethu Tissue/cell culturing system and related methods
US20130330701A1 (en) * 2012-06-12 2013-12-12 Vladimir Rubinstein Physical heart simulator

Also Published As

Publication number Publication date
WO2015108869A2 (fr) 2015-07-23
US20170009207A1 (en) 2017-01-12

Similar Documents

Publication Publication Date Title
WO2015108869A3 (fr) Compartiment cardiaque in vitro
WO2017180915A3 (fr) Répresseurs à base de crispr/cas9 pour inactiver des cibles géniques in vivo et procédés d'utilisation
WO2016085632A3 (fr) Mesures microfluidiques de la réponse d'un organisme à un médicament
AU2015329974A8 (en) GaINAc phosphoramidites, nucleic acid conjugates thereof and their use
SG10201810723VA (en) Improved t cell compositions
WO2016109840A3 (fr) Compositions et procédés pour une efficacité élevée de l'édition du génome in vivo
WO2016015058A3 (fr) Procédés de détermination de types de tissus et/ou de cellules permettant d'obtenir de l'adn sans cellules, et procédés d'identification d'une maladie ou d'un trouble les employant
WO2016161155A3 (fr) Incubateur de culture cellulaire automatisé
WO2017044857A3 (fr) Méthodes et compositions pour le traitement du glaucome
WO2015187809A3 (fr) Techniques permettant d'isoler une partie d'un service informatique en ligne
WO2015195483A3 (fr) Graphène et matériaux de type graphène utilisés pour la manipulation du potentiel de membrane cellulaire
WO2015002724A3 (fr) Cellules β dérivées de cellules souches et compositions et procédés pour générer ces cellules
WO2016109813A3 (fr) Modèles de prédiction de la toxicité neurale basés sur des cellules souches pluripotentes humaines
MX2011011942A (es) Metodos para el diagnostico de trastornos oncologicos usando intercambiadores epimetabolicos, moleculas intracelulares multidimensionales, o influyentes ambientales.
EP4287191A3 (fr) Méthodes et compositions reposant sur le hla et leurs utilisations
WO2015168161A3 (fr) Analyse de l'expression de gènes de cellules isolées multiplexées par commutation de matrice et fragmentation et étiquetage (tagmentation)
MX2016011812A (es) Uso in vivo e in vitro de grafeno.
WO2016069142A3 (fr) Dispositifs de mesure de la fonction contractile, systèmes associés et procédés d'utilisation correspondants
EP3523639A4 (fr) Réactifs de détection et agencements d'électrodes pour éléments de test de diagnostic multi-analytes, ainsi que leurs procédés d'utilisation
WO2015153772A3 (fr) Complexe zirconium 89-oxine utilisé en tant qu'agent de marquage de cellule pour tomographie par émission de positrons
MY193650A (en) Extracellular matrix compositions
WO2016014342A3 (fr) Oligopeptides dérivés de ang-(1-7) et leurs procédés d'utilisation et de production
EP3732286A4 (fr) Génération de cellules pluripotentes induites par activation de crispr
EP3692327A4 (fr) Analyse pour permettre une réduction des contraintes dans le matériau testé
WO2016191179A8 (fr) Modèles in vitro de thérapie régénérative des cellules musculaires et utilisations de ceux-ci

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15737178

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 15737178

Country of ref document: EP

Kind code of ref document: A2