WO2015103317A1 - Therapeutic inhibitory compounds - Google Patents

Therapeutic inhibitory compounds Download PDF

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Publication number
WO2015103317A1
WO2015103317A1 PCT/US2014/072851 US2014072851W WO2015103317A1 WO 2015103317 A1 WO2015103317 A1 WO 2015103317A1 US 2014072851 W US2014072851 W US 2014072851W WO 2015103317 A1 WO2015103317 A1 WO 2015103317A1
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WO
WIPO (PCT)
Prior art keywords
methyl
alkyl
chloro
oxopyridin
benzyl
Prior art date
Application number
PCT/US2014/072851
Other languages
French (fr)
Inventor
Andrew Mcdonald
Original Assignee
Lifesci Pharmaceuticals, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lifesci Pharmaceuticals, Inc. filed Critical Lifesci Pharmaceuticals, Inc.
Priority to CN201480076616.5A priority Critical patent/CN106061480B/en
Priority to US14/800,631 priority patent/US10266515B2/en
Priority to KR1020167020567A priority patent/KR20160106627A/en
Priority to MX2016008688A priority patent/MX2016008688A/en
Priority to EA201691354A priority patent/EA032713B1/en
Priority to AU2014373735A priority patent/AU2014373735B2/en
Priority to EP14877414.4A priority patent/EP3089746A4/en
Priority to CA2935683A priority patent/CA2935683A1/en
Priority to BR112016015449A priority patent/BR112016015449A8/en
Priority to JP2016544593A priority patent/JP6759100B2/en
Publication of WO2015103317A1 publication Critical patent/WO2015103317A1/en
Priority to KR1020177004192A priority patent/KR102286305B1/en
Priority to EP15822307.3A priority patent/EP3169325B1/en
Priority to EA201790170A priority patent/EA035410B1/en
Priority to BR112017000816A priority patent/BR112017000816A2/en
Priority to CA2954814A priority patent/CA2954814A1/en
Priority to PCT/US2015/040659 priority patent/WO2016011209A1/en
Priority to JP2017501403A priority patent/JP6951970B2/en
Priority to MX2017000450A priority patent/MX2017000450A/en
Priority to CN201580049953.XA priority patent/CN107072985B/en
Priority to AU2015289643A priority patent/AU2015289643B2/en
Priority to US15/042,102 priority patent/US9611252B2/en
Priority to US15/470,736 priority patent/US10259803B2/en
Priority to US16/373,391 priority patent/US20200031799A1/en
Priority to US16/373,428 priority patent/US11021463B2/en
Priority to AU2020201550A priority patent/AU2020201550A1/en
Priority to JP2020085352A priority patent/JP6982343B2/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/10—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing aromatic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A61K31/403—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
    • A61K31/4035—Isoindoles, e.g. phthalimide
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/415—1,2-Diazoles
    • A61K31/416—1,2-Diazoles condensed with carbocyclic ring systems, e.g. indazole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
    • A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/4709—Non-condensed quinolines and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/472—Non-condensed isoquinolines, e.g. papaverine
    • A61K31/4725—Non-condensed isoquinolines, e.g. papaverine containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems

Definitions

  • vascular permeability is important in regulating the passage of small molecules or blood cells between blood vessels and surrounding tissues.
  • Vascular permeability can vary with the physiological states of tissues such as during inflammation, changes in blood pressure, and fluctuations in ion and nutrient gradients.
  • the junctions between the endothelial cells that line blood vessels are the immediate controllers of vascular permeability.
  • the strength of these junctions is tightly regulated by the kinin-kallikrein system of polypeptides and enzymes. Abnormalities in the kinin-kallikrein system can lead to a range of pathologies including angioedema, macular edema and brain edema.
  • the kinin-kallikrein system (“contact system”) is responsible for the production of the peptide bradykinin, the proximal molecular mediator of increased vascular permeability (Busse PJ and Buckland MS, Clin. Exp. Allergy, 43(4), 385-94, 2013), blood vessel dilation, inflammation (Bjorkqvist J et al., Thromb. Haemost. 110(3), 399-407, 2013), and pain (Petho G and Reeh PW, Physiol. Rev., 92(4), 1699-775, 2012).
  • FXII zymogen XII
  • FXIIa catalyzes the conversion of prekallikrein to kallikrein, the activated form, which is expressed primarily by hepatocytes in the liver.
  • Activated kallikrein then acts via a positive feedback mechanism to catalyze further conversion of FXII to FXIIa, a process that results in sufficient kallikrein production to drive downstream processes.
  • Kallikrein is a trypsin-like serine protease enzyme.
  • Serine proteases cleave peptide bonds in proteins through a mechanism that involves the binding of a serine residue with the target molecule at the cleavage site.
  • Kallikrein cleaves high molecular weight kinogen (HK) to produce bradykinin. Bradykinin then binds to the bradykinin 2R receptors (BK2R) on endothelial cells to trigger an increase in vascular permeability.
  • Angioedema is a potentially fatal blood disorder characterized by swelling that may occur in the face, gastrointestinal tract, extremities, genitals and upper airways. Angioedema attacks begin in the deeper layers of the skin and mucous membranes with localized blood vessel dilatation and increased permeability.
  • Symptoms of the disease result from the leakage of plasma from blood vessels into surrounding tissues.
  • HAE results from mutations in the genes that code for elements of the coagulation and inflammation pathways.
  • the three forms of HAE are distinguished by their underlying causes and levels of the CI -esterase inhibitor (Cl-INH, serpin peptidase inhibitor, clade G, member 1) protein in the blood, which inhibits the activity of plasma kallikrein.
  • Cl-INH serpin peptidase inhibitor
  • clade G member 1
  • type I patients have insufficient levels of functional Cl-INH
  • type II patients have dysfunctional Cl-INH.
  • type I and II affect men and women at equal rates
  • type III which primarily affects women, results from a mutation in coagulation factor XII (Hageman factor; HAE-FXII).
  • the underlying causes of type I and II HAE are autosomal dominant mutations in Cl-INH gene (SERPING1 gene) on chromosome 11 (1 lql2-ql 3.1).
  • CIINH accounts for 90% of inhibition of FXIIa and 50% of inhibition of plasma kallikrein (Pixley RA et al., J. Biol. Chem., 260, 1723-9, 1985; Schapira M et al., Biochemistry, 20, 2738-43, 1981).
  • Cl-INH also inactivates prekallikrein (Colman RW et al, Blood, 65, 311-8, 1985).
  • Cl-INH levels are normal, its activity blocks FXIIa from converting pre -kallikrein to kallikrein and blocks kallikrein's conversion to HK, thus preventing the production of bradykinin and the edemic episodes.
  • Cl-INH levels are low, or levels of dysfunction Cl-INH high, this inhibition fails and the pathogenic process ensues.
  • plasma kallikrein may contribute to non-hereditary angioedema, high altitude cerebral edema, cytotoxic cerebral edema, osmotic cerebral edema, diabetic macular edema (DME), clinically significant macular edema, cystoid macular edema (CME, Gao BB, Nat Med., 13(2), 181-8, 2007), retinal edema, radiation induced edema, lymph edema, glioma-associated edema, allergic edema e.g. airflow obstruction in chronic allergic sinusitis or perennial rhinitis.
  • retinopathy and diabetic retinopathy include retinopathy and diabetic retinopathy (Liu J and Feener EP, Biol. Chem. 394(3), 319-28, 2013), proliferative and non-pro liferative retinopathy (Liu J et al, Invest. Ophthalmol. Vis.
  • CME following cataract extraction
  • CME induced by cryotherapy CME induced by uveitis
  • CME following vascular occlusion e.g., central retinal vein occlusion, branch retinal vein occlusion or hemiretinal vein occlusion
  • complications related to cataract surgery in diabetic retinopathy hypertensive retinopathy (JA Phillips et al., Hypertension, 53, 175-181, 2009)
  • retinal trauma dry and wet age-related macular degeneration (AMD), ischemic reperfusion injuries (C Storoni et al., JPET, 381, 849-954, 2006), e.g., in a variety of contexts associated with tissue and/or organ transplantation.
  • AMD age-related macular degeneration
  • ischemic reperfusion injuries C Storoni et al., JPET, 381, 849-954, 2006
  • kinin-kallikrein system may contribute to focal cerebral ischemia, damage in surgically- induced brain injury, global cerebral ischemia, spinal cord injury (Radulovic M et al., J. Neuropathol. Exp. Neurol., 72(11), 1072-89, 2013), pain, ischemia, neurological and cognitive deficits, deep vein thrombosis (Bird JE et al., Thromb. Haemost. 2012 Jun; 107(6):1141-50), stroke, myocardial infarction (Konings J et al., Thromb.
  • ischemic stroke e.g., cerebral stroke or subarachnoid stroke
  • intracerebral hemorrhage e.g., cerebral stroke or subarachnoid stroke
  • hemorrhagic transformation of ischemic stroke cerebral trauma associate with injury or surgery, brain aneurysm, arterio-venous malformation, reduction of blood losses during surgical procedures (e.g., cardiothoracic surgery, such as cardiopulmonary bypass or coronary artery bypass grafting), blood coagulation disorders such as thrombosis, itch, disorders with an inflammation component (such as multiple sclerosis; Ma D et al., Blood 2013), epilepsy (Simoes PS et al., Neurochem.
  • astrocyte-activation related diseases e.g., Alzheimer's disease or multiple sclerosis; Burda JE, Glia., 61(9), 1456-70, 2013
  • Parkinson's disease amyotrophic lateral sclerosis, Creutzfeld- Jacob disease
  • stroke epilepsy and trauma (e.g., brain trauma), airflow obstruction in acute asthma; serositis associated with systemic lupus erythematosus (SLE) and other diseases.
  • SLE systemic lupus erythematosus
  • plasma kallikrein inhibitors are considered to be useful in the treatment of other edemas such as macular edema and brain edema, and retinopathy, e.g., retinopathy associated with diabetes and/or hypertension.
  • retinopathy e.g., retinopathy associated with diabetes and/or hypertension.
  • plasma kallikrein inhibitors may also be effective in the treatment of edema formation in diseases, e.g., edema formation related to ischemic reperfusion injuries.
  • Preferred compounds should have little affinity for other proteases. They should be well absorbed from the gastrointestinal tract, be sufficiently metabolically stable and possess favorable pharmacokinetic properties. They should be non-toxic and demonstrate few side-effects.
  • the ideal drug candidate will be able to exist in a physical form that is stable, non-hygroscopic and easily formulated.
  • Design of small molecule drugs targeting plasma kallikrein may yield identification of compounds which target serine proteases similar to kallikrein such as Factor XIa, Factor Xlla, Factor Vila and related proteases also involved in blood coagulation cascades and vascular permeability pathways.
  • the invention provides a compound of the invention which is a compound of Formula I:
  • B is absent or is (Ci-C3)alkyl
  • E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C 6 )alkyl, (C 3 -C 6 )carbocyclyl, (C 3 -C 6 ) carbocyclyl(Ci-C 6 )alkyl, (Ci-C 6 )alkoxy, halo(Ci-C 6 )alkoxy, (C 2 - Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C 2 -C 6 )alkanoyloxy, -NR a R b , and -C
  • R a is H, (Ci-C 6 )alkyl, or (Ci-C 6 )alkanolyl
  • R b is H, (Ci-C 6 )alkyl, or (Ci-C 6 )alkanolyl;
  • the invention also provides a compound of the invention which is a compound of Formula II:
  • E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C 6 )alkyl, (C 3 -C 6 )carbocyclyl, (C 3 -C 6 ) carbocyclyl(Ci-C 6 )alkyl, (Ci-C 6 )alkoxy, halo(Ci-C 6 )alkoxy, (C 2 - Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C 2 -C 6 )alkanoyloxy, -NR a R b , and -C
  • the invention also provides a pharmaceutical composition comprising a compound of Formula I or
  • Formula II or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, diluent, or carrier.
  • the invention also provides a method of treating a disease or condition in an animal wherein inhibition of plasma kallikrein is indicated comprising administering a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, to the animal.
  • the invention also provides a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, for use in medical therapy.
  • the invention also provides a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, for the prophylactic or therapeutic treatment of a disease or condition wherein inhibition of plasma kallikrein is indicated.
  • the invention also provides the use of a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, to prepare a medicament for treatment of a disease or condition in an animal wherein inhibition of plasma kallikrein is indicated.
  • the invention also provides processes and intermediates disclosed herein that are useful for preparing a compound of Formula I or Formula II or a salt thereof.
  • references in the specification to "one embodiment”, “an embodiment”, etc., indicate that the embodiment described may include a particular aspect, feature, structure, moiety, or characteristic, but not every embodiment necessarily includes that aspect, feature, structure, moiety, or characteristic. Moreover, such phrases may, but do not necessarily, refer to the same embodiment referred to in other portions of the specification. Further, when a particular aspect, feature, structure, moiety, or characteristic is described in connection with one embodiment, it is within the knowledge of one skilled in the art to affect or connect such aspect, feature, structure, moiety, or characteristic with other embodiments, whether or not explicitly described, and such combinations are envisaged and are part of the present invention.
  • the term “and/or” means any one of the items, any combination of the items, or all of the items with which this term is associated.
  • the phrases “one or more” and “at least one” are readily understood by one of skill in the art, particularly when read in context of its usage.
  • the phrase “one or more” can mean one, two, three, four, five, six, ten, 100, or any upper limit approximately two or ten times higher than a recited lower limit or element (e.g., typically one or two).
  • one or more substituents on a phenyl ring refers to one to five, or one to four, for example if the phenyl ring is currently disubstituted.
  • the term “about” can refer to a variation of ⁇ 5%, ⁇ 10%, ⁇ 20%, or ⁇ 25% of the value specified. For example, “about 50" percent can in some embodiments carry a variation from 45 to 55 percent.
  • the term “about” can include one or two integers greater than and/or less than a recited integer at each end of the range. Unless indicated otherwise herein, the term “about” is intended to include values, e.g., weight percentages, proximate to the recited range that are equivalent in terms of the functionality of the individual ingredient, the composition, or the embodiment.
  • the term about can also modify the end-points of a recited range as discuss above in this paragraph.
  • the invention may encompass not only the main group, but also the main group absent one or more of the group members.
  • the invention therefore envisages the explicit exclusion of any one or more of members of a recited group. Accordingly, provisos may apply to any of the disclosed categories or embodiments whereby any one or more of the recited elements, species, or embodiments, may be excluded from such categories or embodiments, for example, for use in an explicit negative limitation.
  • an “effective amount” refers to an amount effective to treat a disease, disorder, and/or condition, or to bring about a recited effect.
  • an effective amount can be an amount effective to reduce the progression or severity of the condition or symptoms being treated. Determination of a therapeutically effective amount is well within the capacity of persons skilled in the art.
  • the term "effective amount” can include an amount of a compound described herein, or an amount of a combination of compounds described herein, e.g., that is effective to treat or prevent a disease or disorder, or to treat the symptoms of the disease or disorder, in a host.
  • an “effective amount” generally means an amount that provides the desired effect.
  • treating can include (i) preventing a disease, pathologic or medical condition from occurring (e.g., prophylaxis); (ii) inhibiting the disease, pathologic or medical condition or arresting its development; (iii) relieving the disease, pathologic or medical condition; and/or (iv) diminishing symptoms associated with the disease, pathologic or medical condition.
  • the terms “treat”, “treatment”, and “treating” can extend to prophylaxis and/or can include prevent, prevention, preventing, lowering, stopping or reversing the progression or severity of the condition or symptoms being treated.
  • treatment can include medical, therapeutic, and/or prophylactic administration, as appropriate.
  • inhibitor refers to the slowing, halting, or reversing the growth or progression of a disease, infection, condition, or activity of an enzyme.
  • the inhibition can be greater than about 20%, 40%, 60%, 80%, 90%, 95%, or 99%, for example, compared to the growth or progression that occurs in the absence of the treatment or contacting.
  • substituents of the compounds described herein may be present to a recursive degree.
  • "recursive substituent” means that a substituent may recite another instance of itself. Because of the recursive nature of such substituents, theoretically, a large number may be present in any given claim.
  • One of ordinary skill in the art of medicinal chemistry and organic chemistry understands that the total number of such substituents is reasonably limited by the desired properties of the compound intended. Such properties include, by of example and not limitation, physical properties such as molecular weight, solubility or log P, application properties such as activity against the intended target, and practical properties such as ease of synthesis.
  • Recursive substituents are an intended aspect of the invention.
  • One of ordinary skill in the art of medicinal and organic chemistry understands the versatility of such substituents.
  • the total number will be determined as set forth above or as described in this paragraph.
  • recursive substituents are present only to the extent that the molecular mass of the compound is about 400 to about 1600, about 450 to about 1200, about 500 to about 100, about 500 to about 800.
  • recursive substituents are present only to the extent that the molecular mass of the compound is less than 2000, less than 1800, less than 1600, less than 1500, less than 1400, less than 1200, less than 1000, less than 900, less than 800, less than 750, less than 700, or less than about 600.
  • the term "carbocyclyl” used alone or as part of a larger moiety refers to a saturated, partially unsaturated, or aryl ring system having 3 to 20 carbon atoms. In one embodiment, carbocyclyl includes 3 to 12 carbon atoms (C3-C12). In another embodiment, carbocyclyl includes C3-C8, C3-Cio or C5-C10.
  • carbocyclyl as a monocycle, includes C3-C8, C3-C6 or C5-C6.
  • carbocyclyl, as a bicycle includes C7-C12.
  • carbocyclyl, as a spiro system includes C5-C12.
  • Examples of monocyclic carbocyclyls include cyclopropyl, cyclobutyl, cyclopentyl, 1-cyclopent-l- enyl, 1 -cyclopent-2-enyl, l-cyclopent-3-enyl, cyclohexyl, perdeuteriocyclohexyl, 1-cyclohex-l -enyl, 1- cyclohex-2-enyl, l -cyclohex-3-enyl, cyclohexadienyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, cycloundecyl, phenyl, and cyclododecyl; bicyclic carbocyclyls having 7 to 12 ring atoms include [4,3], [4,4], [4,5], [5,5], [5,6] or [6,6] ring systems, for example bicyclo[2.2.1]heptan
  • carbocyclyl includes aryl ring systems as defined herein.
  • carbocycyl also includes cycloalkyl rings (e.g. saturated or partially unsaturated mono-, bi-, or spiro- carbocycles).
  • alkyl refers to a saturated linear or branched-chain hydrocarbon radical.
  • a radical as used herein can be a monoradical, diradical, or multiradical, depending on how many substituents the group includes.
  • the alkyl radical is one to eighteen carbon atoms (Ci-Cis).
  • the alkyl radical is G-C12, C1-G0, Ci-Cs, Ci-Ce, C1-C5, C1-C4 or C1-C3.
  • alkyl groups include methyl (Me, -CH 3 ), ethyl (Et, -CH2CH3), 1 -propyl (n-Pr, n-propyl, -CH 2 CH 2 CH 3 ), 2-propyl (i- Pr, i-propyl, -CH(CH 3 ) 2 ), 1 -butyl (n-Bu, n-butyl, -CH2CH2CH2CH3), 2-methyl-l -propyl (i-Bu, i-butyl, -
  • alkenyl denotes a linear or branched-chain hydrocarbon radical with at least one carbon-carbon double bond.
  • An alkenyl includes radicals having "cis” and “trans” orientations, or alternatively, "E” and “Z” orientations.
  • the alkenyl radical is two to eighteen carbon atoms (C2-C10).
  • alkynyl refers to a linear or branched hydrocarbon radical with at least one carbon-carbon triple bond.
  • the alkynyl radical is two to eighteen carbon atoms (C2-C10).
  • the alkynyl radical is C2-C8, C2-C6 or C2-C3. Examples include, but are not limited to, ethynyl (-OCH), prop-l-ynyl (-OCCH3), prop-2-ynyl (propargyl, -CH 2 C ⁇ CH), but-l-ynyl, but-2-ynyl and but-3- ynyl.
  • alkoxy refers to a linear or branched radical represented by the formula -OR in which R is alkyl, alkenyl, alkynyl or carbocycyl. Alkoxy groups include methoxy, ethoxy, propoxy, isopropoxy, and cyclopropoxy.
  • haloalkyl refers to an alkyl as defined herein that is substituted with one or more (e.g. 1, 2, 3, or 4) halo groups.
  • aryl used alone or as part of a larger moiety as in “arylalkyl”, “arylalkoxy”, or
  • aryloxyalkyl refers to a monocyclic, bicyclic or tricyclic, carbon ring system, that includes fused rings, wherein at least one ring in the system is aromatic.
  • aryl may be used interchangeably with the term "aryl ring".
  • aryl includes groups having 6-12 carbon atoms.
  • aryl includes groups having 6-10 carbon atoms. Examples of aryl groups include phenyl, naphthyl, anthracyl, phenanthrenyl, naphthacenyl, 1,2,3,4-tetrahydronaphthalenyl, lH-indenyl, 2,3-dihydro- lH-indenyl, and the like.
  • a particular aryl is phenyl.
  • aryl includes indanyl, naphthyl, and tetrahydronaphthyl, and the like, where the radical or point of attachment is on an aromatic ring.
  • heteroaryl used alone or as part of a larger moiety, e.g., “heteroarylalkyl”, or
  • heteroarylalkoxy refers to a monocyclic, bicyclic or tricyclic ring system having 5 to 12 ring atoms, wherein at least one ring is aromatic and contains at least one heteroatom.
  • heteroaryl includes 4-6 membered monocyclic aromatic groups where one or more ring atoms is nitrogen, sulfur or oxygen.
  • heteroaryl includes 5-6 membered monocyclic aromatic groups where one or more ring atoms is nitrogen, sulfur or oxygen.
  • heteroaryl groups include thienyl, furyl, imidazolyl, pyrazolyl, thiazolyl, isothiazolyl, oxazolyl, isoxazolyl, triazolyl, thiadiazolyl, oxadiazolyl, tetrazolyl, thiatriazolyl, oxatriazolyl, pyridyl, pyrimidyl, pyrazinyl, pyridazinyl, triazinyl, tetrazinyl, tetrazolo[l,5-b]pyridazinyl, imidazol[l,2-a]pyrimidinyl, purinyl, benzoxazolyl, benzofuryl, benzothiazolyl, benzothiadiazolyl, benzotriazolyl, benzoimidazolyl, indolyl, l,3-thiazol-2-yl, l,3,4-tri
  • heteroaryl also includes groups in which a heteroaryl is fused to one or more aryl, carbocyclyl, or heterocyclyl rings, where the radical or point of attachment is on the heteroaryl ring.
  • Nonlimiting examples include indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H- quinolizinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl and pyrido[2,3-b]-l,4-oxazin-3(4
  • heterocyclyl refers to a “carbocyclyl” as defined herein, wherein one or more (e.g. 1, 2, 3, or 4) carbon atoms have been replaced with a heteroatom (e.g. O, N, or S).
  • a heterocyclyl refers to a saturated ring system, such as a 3 to 12 membered saturated heterocyclyl ring system.
  • a heterocyclyl refers to a heteroaryl ring system, such as a 5 to 14 membered heteroaryl ring system.
  • heterocyclyl also includes Cs-Csheterocycloalkyl, which is a saturated or partially unsaturated mono-, bi-, or spiro-ring system comprising 3-8 carbons and one or more (1, 2, 3, or 4) heteroatoms.
  • heterocyclyl includes 3-12 ring atoms and includes monocycles, bicycles, tricycles and spiro ring systems, wherein the ring atoms are carbon, and one to five ring atoms is a heteroatom selected from nitrogen, sulfur or oxygen, which is independently optionally substituted by one or more groups.
  • heterocyclyl includes 1 to 4 heteroatoms.
  • heterocyclyl includes 3- to 7- membered monocycles having one or more heteroatoms selected from nitrogen, sulfur or oxygen.
  • heterocyclyl includes 4- to 6-membered monocycles having one or more heteroatoms selected from nitrogen, sulfur or oxygen.
  • heterocyclyl includes 3-membered monocycles.
  • heterocyclyl includes 4-membered monocycles. In another example, heterocyclyl includes 5-6 membered monocycles. In one example, the heterocyclyl group includes 0 to 3 double bonds. Any nitrogen or sulfur heteroatom may optionally be oxidized (e.g. NO, SO, SO2), and any nitrogen heteroatom may optionally be quaternized (e.g. [NRi] + Cl " , [NRi] + OH " ).
  • Example heterocyclyls include oxiranyl, aziridinyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, 1 ,2-dithietanyl, 1,3-dithietanyl, pyrrolidinyl, dihydro-lH-pyrrolyl, dihydrofuranyl, tetrahydrofuranyl, dihydrothienyl, tetrahydrothienyl, imidazolidinyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, 1 , 1 -dioxo-thiomorpholinyl, dihydropyranyl, tetrahydropyranyl,
  • hexahydrothiopyranyl hexahydropyrimidinyl, oxazinanyl, thiazinanyl, thioxanyl, homopiperazinyl, homopiperidinyl, azepanyl, oxepanyl, thiepanyl, oxazepinyl, oxazepanyl, diazepanyl, 1 ,4-diazepanyl, diazepinyl, thiazepinyl, thiazepanyl, tetrahydrothiopyranyl, oxazolidinyl, thiazolidinyl, isothiazolidinyl, 1,1- dioxoisothiazolidinonyl, oxazolidinonyl, imidazolidinonyl, 4,5,6,7-tetrahydro[2H]indazolyl,
  • azabicyclo[2.2.2]hexanyl 2-azabicyclo[3.2.1]octanyl, 8-azabicyclo[3.2.1]octanyl, 2-azabicyclo[2.2.2]octanyl, 8-azabicyclo[2.2.2]octanyl, 7-oxabicyclo[2.2.1]heptane, azaspiro[3.5]nonanyl, azaspiro[2.5]octanyl, azaspiro[4.5]decanyl, l-azaspiro[4.5]decan-2-only, azaspiro[5.5]undecanyl, tetrahydroindolyl,
  • octahydroindolyl tetrahydroisoindolyl, tetrahydroindazolyl, 1,1-dioxohexahydrothiopyranyl and 2- oxopyridin-l(2H)-yl.
  • Examples of 5-membered heterocyclyls containing a sulfur or oxygen atom and one to three nitrogen atoms are thiazolyl, including thiazol-2-yl and thiazol-2-yl N-oxide, thiadiazolyl, including l,3,4-thiadiazol-5-yl and l,2,4-thiadiazol-5-yl, oxazolyl, for example oxazol-2-yl, and oxadiazolyl, such as l,3,4-oxadiazol-5-yl, and l,2,4-oxadiazol-5-yl.
  • Examples of 5-membered ring heterocyclyls containing 2 to 4 nitrogen atoms include imidazolyl, such as imidazol-2-yl; triazolyl, such as l,3,4-triazol-5-yl; l,2,3-triazol-5- yl, l,2,4-triazol-5-yl, and tetrazolyl, such as lH-tetrazol-5-yl.
  • Example benzo-fused 5-membered heterocyclyls are benzoxazol-2-yl, benzthiazol-2-yl and benzimidazol-2-yl.
  • 6-membered heterocyclyls contain one to three nitrogen atoms and optionally a sulfur or oxygen atom, for example pyridyl, such as pyrid-2-yl, pyrid-3-yl, and pyrid-4-yl; pyrimidyl, such as pyrimid-2-yl and pyrimid-4-yl; triazinyl, such as l,3,4-triazin-2-yl and l,3,5-triazin-4-yl; pyridazinyl, in particular pyridazin-3-yl, and pyrazinyl.
  • pyridyl such as pyrid-2-yl, pyrid-3-yl, and pyrid-4-yl
  • pyrimidyl such as pyrimid-2-yl and pyrimid-4-yl
  • triazinyl such as l,3,4-triazin-2-yl and l,3,5-tri
  • pyridine N-oxides and pyridazine N-oxides and the pyridyl, pyrimid-2-yl, pyrimid-4-yl, pyridazinyl and the l,3,4-triazin-2-yl groups are other example heterocyclyl groups.
  • partially unsaturated refers to a ring moiety that includes at least one double or triple bond between ring atoms but the ring moiety is not aromatic.
  • the atom to which the bond is attached includes all stereochemical possibilities.
  • a bond in a compound formula herein is drawn in a defined stereochemical manner (e.g. bold, bold-wedge, dashed or dashed-wedge)
  • a bond in a compound formula herein is drawn in a defined stereochemical manner (e.g. bold, bold-wedge, dashed or dashed-wedge)
  • the atom to which the stereochemical bond is attached is enriched in the absolute stereoisomer depicted unless otherwise noted.
  • the compound may be at least 51% the absolute stereoisomer depicted.
  • the compound may be at least 60% the absolute stereoisomer depicted.
  • the compound may be at least 80%> the absolute stereoisomer depicted.
  • the compound may be at least 90% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 95 the absolute stereoisomer depicted. In another embodiment, the compound may be at least 99% the absolute stereoisomer depicted.
  • (Ci-Ce)alkyl can be methyl, ethyl, propyl, isopropyl, butyl, iso-butyl, sec-butyl, pentyl, 3-pentyl, or hexyl;
  • (C3-C6)cycloalkyl can be cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl;
  • C3- C6)cycloalkyl(Ci-C6)alkyl can be cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl,
  • (Ci- Ce)alkoxy can be methoxy, ethoxy, propoxy, isopropoxy, butoxy, iso-butoxy, sec-butoxy, pentoxy, 3-pentoxy, or hexyloxy;
  • (C2-Ce)alkenyl can be vinyl, allyl, 1-propenyl, 2-propenyl, 1-butenyl, 2-butenyl, 3-butenyl, 1,- pentenyl, 2-pentenyl, 3-pentenyl, 4-pentenyl, 1 - hexenyl, 2-hexenyl, 3-hexenyl, 4-hexenyl, or 5-hexenyl;
  • (C2- C6)alkynyl can be ethynyl, 1-propynyl, 2-propynyl
  • halo(Ci- Ce)alkoxy can be fluoromethoxy, trifluoromethoxy, 2-fluoroethoxy, 2-chloroethoxy, 2,2,2-trifluoroethoxy, or pentafluoroethoxy
  • hydroxy(Ci-C6)alkyl can be hydroxymethyl, 1 -hydroxy ethyl, 2-hydroxyethyl, 1 - hydroxypropyl, 2-hydroxypropyl, 3-hydroxypropyl, 1 -hydroxybutyl, 4-hydroxybutyl, 1 -hydroxypentyl, 5- hydroxypentyl, 1 -hydroxyhexyl, or 6-hydroxyhexyl
  • (Ci-C6)alkoxycarbonyl can be methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl
  • (C2-Ce)alkanoyloxy can be acetoxy, propanoyloxy, butanoyloxy, isobutanoyloxy, pentanoyloxy, or hexanoyloxy
  • aryl can be phenyl, indenyl, or naphthyl
  • heteroaryl can be furyl, imidazolyl, triazolyl, triazinyl, oxazoyl, isoxazoyl, thiazolyl, isothiazoyl, pyrazolyl, pyrrolyl, pyrazinyl, tetrazolyl, pyridyl, (or its N-oxide), thienyl, pyrimidinyl (or its N-oxide), indolyl, isoquinolyl (or its N-oxide) or quinolyl (or its N-oxide).
  • J is aryl or heteroaryl, which aryl or heteroaryl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3- C 6 )carbocyclyl(Ci-C 6 )alkyl, (Ci-C 6 )alkoxy, halo(Ci-C 6 )alkoxy, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (G- Ce)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C 2 -
  • each R e is independently H or (Ci-Ce)alkyl
  • D is hydroxy(G-C6)alkyl. In an embodiment D is alkoxy(G-C3)alkyl.
  • F is -C(COOR a )-. In an embodiment F is -C(CF3)-. In an embodiment J is -0-NH-C(NH)-NH 2 . In an embodiment J is -C(NH)-NH 2 .
  • compounds of the invention have the Formula I-aa or I-bb:
  • E is phenyl.
  • E is pyridinyl.
  • E is indazolyl.
  • E is piperidinyl.
  • E is pyrazinyl.
  • E is indolyl.
  • E is pyrrolidinyl.
  • E is piperazinyl.
  • E is pyrazolyl.
  • E is 5-oxo-4,5-dihydropyrazinyl.
  • B is absent or is (Ci-C3)alkyl
  • C is phenyl or a 6-membered heteroaryl ring, which phenyl or 6-membered heteroaryl ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C 2 -C 6 )alkanoyloxy, (Ci-C 6 )alkyl-NR a R b , -NR a R b , and -C
  • E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C 6 )alkyl, (C 3 -C 6 )carbocyclyl, (C 3 -C 6 ) carbocyclyl(Ci-C 6 )alkyl, (Ci-C 6 )alkoxy, halo(Ci-C 6 )alkoxy, (C 2 - Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C 2 -C 6 )alkanoyloxy, -NR a R b , and -C
  • R a is H, (Ci-C 6 )alkyl, or (Ci-C 6 )alkanolyl
  • R b is H, (Ci-C 6 )alkyl, or (Ci-C 6 )alkanolyl;
  • a specific value for A is a piperidine, indazole, morpholine, piperazine, oxazolidine, imidazole, or pyrrolidine, that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci- Ce)alkoxy, (C 2 -Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl,
  • a specific value for A is a piperidine, indazole, morpholine, piperazine, oxazolidine, imidazole, or pyrrolidine, that is substituted with oxo and that is optionally substituted with one or more groups
  • a specific value for A is a 2-oxopiperidin-l-yl, pyrazole-l-yl, 3-oxomorpholino, 2-oxooxazolidin-3- yl, 2-oxoimidazolidin-l-yl, or 2-oxopyrrolidin-l -yl.
  • the heterocyclic ring of A is substituted with a fluoro group.
  • a specific value for B is C3 ⁇ 4.
  • a specific value for C is phenyl or a 6-membered heteroaryl ring, which phenyl or 6-membered heteroaryl ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci- Ce)alkoxy, (C 2 -Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl,
  • a specific value for C is pyridine, pyrimidine, pyrazine, pyridazine, or triazine, wherein any pyridine, pyrimidine, pyrazine, pyridazine, or triazine is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C 6 )alkoxy, halo(Ci-C 6 )alkoxy, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (Ci-C 6 )alkanoyl, halo(Ci-C 6 )alkyl, hydroxy(Ci-C 6 )alkyl, (Ci-C 6 )alkoxycarbonyl, (C
  • a specific value for C is phenyl, aminophenyl, fluorophenyl, chlorophenyl, cyanophenyl,
  • C is a bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C 2 -Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C 6 )alkyl, hydroxy(Ci-C 6 )alkyl, (G-C 6 )alkoxycarbonyl, (C 2 -C 6 )alkanoyloxy, -(Ci-C 6 )alkyl-COOH, - (Ci-C 6 )alkyl-CO-(Ci-C 6 )alk
  • C is a monocyclic carbocyclic ring or a monocyclic heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C 6 )alkoxy, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (Ci-C 6 )alkanoyl, halo(Ci-C 6 )alkyl, hydroxy(Ci-C 6 )alkyl, (Ci-C 6 )alkoxycarbonyl, (C 2 -C 6 )alkanoyloxy, -NR a R b
  • or J is a monocyclic heterocyclic ring, which heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C 6 )carbocyclyl(Ci-C 6 )alkyl, (G-C 6 )alkoxy, halo(Ci-C 6 )alkoxy, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (Ci- Ce)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C 2 -C6)alkanoyloxy, -NR a R b , - (Ci-C 6 )alkyl-NR a R b ,
  • each R e is independently H or (Ci-Ce)alkyl
  • C is a monocyclic carbocyclic ring or a monocyclic heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(G- C 6 )alkyl, (Ci-C 6 )alkoxy, halo(Ci-C 6 )alkoxy, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (Ci-C 6 )alkanoyl, halo(G- C 6 )alkyl, hydroxy(Ci-C 6 )alkyl, (Ci-C 6 )alkoxycarbonyl, (C 2 -C 6 )alkanoyloxy, -NR
  • C is pyridyl. In a particular embodiment, C is phenyl. In another particular embodiment, C is quinoline or quinoline substituted with halo, such as fluoro, or a (Ci-C6)alkyl, such as methyl.
  • C is substituted with -(Ci-C6)alkyl-NR a R b .
  • C is substituted with aminomethyl.
  • C is substituted with -(Ci-C6)alkyl-NR a R b .
  • C is substituted with acetamidomethyl.
  • C is substituted with -(Ci-C6)alkyl-CO-NR a R b .
  • C is substituted with 2-amino-2-oxoethyl.
  • C is substituted with 3-amino-3-oxopropyl.
  • C is substituted with -(Ci-C6)alkyl-CO-(Ci-C6)alkoxy.
  • C is substituted with -CH 2 - COO-Me.
  • C is substituted with -(CH 2 ) 2 -COO-Me.
  • C is substituted with -(Ci-C6)alkyl-NH-C(0)-NR a R b .
  • C is substituted with -CH 2 - NH-CO-NH 2 .
  • C is substituted with -(Ci-C6)alkoxy-CO-NR a R b .
  • C is substituted with -0-CH 2 -CO-NH 2 .
  • C is substituted with -(Ci- C6)alkyl-NR a R b .
  • C is substituted with methylacetamidomethyl.
  • C is substituted with 1 -acetamidoethyl.
  • C is as defined herein for Formula II.
  • C phenyl optionally substituted as defined herein for Formula II.
  • C is pyridyl, optionally substituted as defined herein for Formula II.
  • J is as defined herein for Formula II.
  • J is indole.
  • J is 3-chloro-6-fluoro-lH-indol-5-yl.
  • J is 3-chloro-lH-indol-5-yl.
  • J is indazolyl.
  • J is 5-chloro-lH-indazol-3-yl.
  • J is pyrrolo[2,3-b]pyridinyl.
  • J is 3-chloro-lH-pyrrolo [2,3-b]pyridin-5-yl.
  • J is a monocyclic heterocyclic ring.
  • J is an amino-dimethylpyridinyl group such as 6-amino-2,4-dimethylpyridin-3-yl.
  • E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C 6 )alkyl, (C 3 -C 6 )carbocyclyl, (C 3 -C 6 ) carbocyclyl(Ci-C 6 )alkyl, (Ci-C 6 )alkoxy, halo(Ci-C 6 )alkoxy, (C 2 - Ce)alkenyl, (C 2 -C6)alkynyl, (Ci-C6)alkanoyl, halo(
  • a specific compound of Formula II is a compound of Formula lib: Formula lib
  • a specific compound of Formula I is a compound of Formula lb:
  • a specific compound of Formula I is a compound of Formula Ic:
  • a specific value for C is a quinoline that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6)
  • carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C 2 -Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C 2 -C6)alkanoyloxy, -NR a R b , and - C( 0)NR a R b .
  • a specific value for C is a 6-quinoline that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6)
  • carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C 2 -Ce)alkenyl, (C 2 -Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C 2 -C6)alkanoyloxy, -NR a R b , and - C( 0)NR a R b .
  • a specific value for C is a 2-methyl-6-quiolinyl.
  • a specific value for D is (Ci-C3)alkyl. Another specific value for D is methylene.
  • a specific value for E is pyrazole, pyridine, indole, phenyl, morpholine, pyrimidine, pyrazine, pyradizine, 2-oxooxazolidine, pyrrolidine, 2-oxopyrrolidine, piperazine, oxopiperazine, oxopyridine, oxopyrimidine, oxopiperidine, oxomorpholine, azaspiro[3,4]octane, 5-thia-2-azaspiro[3,4]octane, hydroxypyridine, indazole, lH-pyrrolo[2,3-b]pyridine, or hydroxypyrimidine, wherein E is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C 3 -C 6 )carbocyclyl, (C 3 -C 6 ) carbocyclyl(Ci
  • a specific value for E is pyrazole, pyridine, or phenyl.
  • a specific value for E is pyrazole.
  • a specific value for E is pyridine.
  • G Another specific value for G is methylene.
  • a specific value for J is aryl.
  • Another specific value for J is heteroaryl.
  • the aryl or heteroaryl can be substituted.
  • the substituents are one or more halo, amino, or methyl groups, or a combination thereof.
  • J is 8-10 membered heteroaryl ring.
  • the heteroaryl ring can be substituted, for example, with one or more halo, amino, or methyl groups, or a combination thereof.
  • J is monocyclic heterocyclic ring.
  • the monocyclic heterocyclic ring is substituted with one or more halo, amino, or methyl groups, or a combination thereof.
  • a specific value for J is chloro-indazole-3-yl. Another specific value for J is 5-chloro-indazole-3-yl.
  • J further include 1 -aminoisoquinolin-6-yl, 3-chloro-6-fluoro-lH-indol-5-yl, 6- amino-2,4-dimethylpyridin-3-yl, 3-chloro-4-fluoro-lH-indol-5-yl, or 3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl, which groups can be optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C 6 )alkoxy, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (Ci-C 6 )alkanoyl, halo(
  • a specific value for R a or R b is H. Another specific value for R a or R b is acetyl. Another specific value for R a or R b is 2-hydroxy-acetyl. Another specific value for R a or R b is 2-amino-acetyl. Another specific value for R a or R b is methoxyformyl. Another specific value for R a or R b is cyclopropylformyl. Another specific value for R a or R b is cyclopropyl.
  • Xi is N, and X2, X3 and X4 are CH.
  • Xi and X3 are N, and X2 and X4 are CH.
  • Xi and X4 are N, and X2 and X3 are CH.
  • Xi and X2 are N, and X3 and X4 are CH.
  • a specific compound of the invention can be selected from Table 1.
  • a specific compound can be further selected from:
  • a specific compound can also be selected from:
  • a specific compound can yet further be selected from:
  • a specific compound can also be selected from:
  • a specific compound of the invention can also be selected from:
  • Carboxylic acid C-l is coupled with a requsite amine to provide C-2.
  • Examples of coupling reactions include, but are not limited to, Suzuki reaction, Buchwald reaction, Ullmann reaction, SN(AR), and the like.
  • Compound C-2 is then converted to the target compound of Formula II.
  • a salt of a compound of Formula I or II can be useful as an intermediate for isolating or purifying a compound of Formula I or II. Additionally, administration of a compound of Formula I or II as a pharmaceutically acceptable acid or base salt may be appropriate.
  • pharmaceutically acceptable salts are organic acid addition salts formed with acids which form a physiological acceptable anion, for example, tosylate, methanesulfonate, acetate,
  • Suitable inorganic salts may also be formed, including hydrochloride, sulfate, nitrate, bicarbonate, and carbonate salts.
  • the compounds may also be isolated as solvates.
  • salts may be obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound such as an amine with a suitable acid affording a physiologically acceptable anion.
  • a sufficiently basic compound such as an amine
  • a suitable acid affording a physiologically acceptable anion.
  • Alkali metal (for example, sodium, potassium or lithium) or alkaline earth metal (for example calcium) salts of carboxylic acids can also be made.
  • the compounds of Formula I or II can be formulated as pharmaceutical compositions and
  • a mammalian host such as a human patient in a variety of forms adapted to the chosen route of administration, i.e., orally or parenterally, by intravenous, intramuscular, topical or subcutaneous routes.
  • the present compounds may be systemically administered, e.g., orally, in combination with a pharmaceutically acceptable vehicle such as an inert diluent or an assimilable edible carrier. They may be enclosed in hard or soft shell gelatin capsules, may be compressed into tablets, or may be incorporated directly with the food of the patient's diet.
  • a pharmaceutically acceptable vehicle such as an inert diluent or an assimilable edible carrier.
  • the active compound may be combined with one or more excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like.
  • Such compositions and preparations should contain at least 0.1% of active compound.
  • the percentage of the compositions and preparations may, of course, be varied and may conveniently be between about 2 to about 60%> of the weight of a given unit dosage form.
  • the amount of active compound in such therapeutically useful compositions is such that an effective dosage level will be obtained.
  • the tablets, troches, pills, capsules, and the like may also contain the following: binders such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, fructose, lactose or aspartame or a flavoring agent such as peppermint, oil of wintergreen, or cherry flavoring may be added.
  • a liquid carrier such as a vegetable oil or a polyethylene glycol.
  • any material used in preparing any unit dosage form should be pharmaceutically acceptable and substantially non- toxic in the amounts employed.
  • the active compound may be incorporated into sustained-release preparations and devices.
  • the active compound may also be administered intravenously or intraperitoneally by infusion or injection.
  • Solutions of the active compound or its salts can be prepared in water, optionally mixed with a nontoxic surfactant.
  • Dispersions can also be prepared in glycerol, liquid polyethylene glycols, triacetin, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
  • the pharmaceutical dosage forms suitable for injection or infusion can include sterile aqueous solutions or dispersions or sterile powders comprising the active ingredient which are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or dispersions, optionally encapsulated in liposomes.
  • the ultimate dosage form should be sterile, fluid and stable under the conditions of manufacture and storage.
  • the liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising, for example, water, ethanol, a polyol (for example, glycerol, propylene glycol, liquid
  • the proper fluidity can be maintained, for example, by the formation of liposomes, by the maintenance of the required particle size in the case of dispersions or by the use of surfactants.
  • the prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, buffers or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and/or gelatin.
  • Sterile injectable solutions are prepared by incorporating the active compound in the required amount in the appropriate solvent with various other ingredients enumerated above, as desired, followed by filter sterilization.
  • the preferred methods of preparation are vacuum drying and the freeze drying techniques, which yield a powder of the active ingredient plus any additional desired ingredient present in the previously sterile-filtered solutions.
  • the present compounds may be applied in pure form, i.e., when they are liquids. However, it will generally be desirable to administer them to the skin as compositions or
  • compositions in combination with a dermatologically acceptable carrier, which may be a solid or a liquid.
  • Useful solid carriers include finely divided solids such as talc, clay, microcrystalline cellulose, silica, alumina and the like.
  • Useful liquid carriers include water, alcohols or glycols or water-alcohol/glycol blends, in which the present compounds can be dissolved or dispersed at effective levels, optionally with the aid of non-toxic surfactants.
  • Adjuvants such as fragrances and additional antimicrobial agents can be added to optimize the properties for a given use.
  • the resultant liquid compositions can be applied from absorbent pads, used to impregnate bandages and other dressings, or sprayed onto the affected area using pump-type or aerosol sprayers.
  • Thickeners such as synthetic polymers, fatty acids, fatty acid salts and esters, fatty alcohols, modified celluloses or modified mineral materials can also be employed with liquid carriers to form spreadable pastes, gels, ointments, soaps, and the like, for application directly to the skin of the user.
  • dermato logical compositions for delivering active agents to the skin are known to the art; for example, see U.S. Patent Nos. 4,992,478 (Geria), 4,820,508 (Wortzman), 4,608,392 (Jacquet et al.), and 4,559,157 (Smith et al.).
  • Such dermatological compositions can be used in combinations with the compounds described herein where an ingredient of such compositions can optionally be replaced by a compound described herein, or a compound described herein can be added to the composition.
  • Useful dosages of the compounds of Formula I or II can be determined by comparing their in vitro activity, and in vivo activity in animal models. Methods for the extrapolation of effective dosages in mice, and other animals, to humans are known to the art; for example, see U.S. Pat. No. 4,938,949 (Borch et al.).
  • the amount of the compound, or an active salt or derivative thereof, required for use in treatment will vary not only with the particular salt selected but also with the route of administration, the nature of the condition being treated and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or clinician.
  • a suitable dose will be in the range of from about 0.5 to about 100 mg/kg, e.g., from about 10 to about 75 mg/kg of body weight per day, such as 3 to about 50 mg per kilogram body weight of the recipient per day, preferably in the range of 6 to 90 mg/kg/day, most preferably in the range of 15 to 60 mg/kg/day.
  • the compound is conveniently formulated in unit dosage form; for example, containing 5 to 1000 mg, conveniently 10 to 750 mg, most conveniently, 50 to 500 mg of active ingredient per unit dosage form.
  • the invention provides a composition comprising a compound of the invention formulated in such a unit dosage form.
  • the desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example, as two, three, four or more sub-doses per day.
  • the sub-dose itself may be further divided, e.g., into a number of discrete loosely spaced administrations; such as multiple inhalations from an insufflator or by application of a plurality of drops into the eye.
  • a disease or condition in an animal wherein inhibition of plasma kallikrein is indicated includes the diseases and conditions recited above in the Background section above.
  • a specific condition where inhibition of plasma kallikrein is indicated is genetic angioedema or 'hereditary angioedema' ("HAE").
  • the ability of a compound of the invention to inhibit plasma kallikrein may be determined using pharmacological models which are well known to the art, or using Test A described below.
  • a 10 mM solution of a test compound ('Compound X') was made in DMSO. This solution was serially diluted 1 in 5 in DMSO to yield 2000, 400, 80, 16, 3.2, 0.64, 0.128, 0.0256 and 0.00512 ⁇ solutions, hereby referred to as "100X Compound X DMSO stocks". A control tube containing only DMSO was included. 16 ⁇ . of each 100X DMSO stock dilution was combined with 384 ⁇ . of assay buffer (50 mM Tris- HC1 pH 7.5, 150 mM NaCl, 0.01% Triton X-100) to yield a "4X Compound X buffer stock".
  • assay buffer 50 mM Tris- HC1 pH 7.5, 150 mM NaCl, 0.01% Triton X-100
  • N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrazine-2- carboxamide (HC1 salt, 17.8 mg) was prepared from methyl 6-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)pyrazine-2-carboxylate as described for N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide.
  • Example 17 Preparation of 2-(5-chloro-3-((l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)methyl)-lH-indazol-l -yl)acetic acid
  • Example 18 Preparation of N-((l-(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-l-(4-((2- oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H-pyrazole-4-carboxamide
  • 2-Amino-2-(5-bromopyridin-3-yl)acetate (1.0 g, 4.0 mmol, 1.0 eq) was diluted with dichloromethane (60 mL) and slowly added with NaHCCb (685 mg, 8.1 mmol, 2.0 eq), triethylamine (1.0 mL, 8.1 mmol, 2.0 eq), and di- tert-butyl dicarbonate (1.0 g, 4.8 mmol, 1.2 eq) at rt in sequence. The reaction solution was stirred for 2 h at the same temperature, and slowly added with a saturated aqueous solution of ammonium chloride (100 mL).
  • Example 28 Preparation of methyl 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(5-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)pyridin-3 -yl)acetate
  • Example 29 Preparation of 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)pyridin-3-yl)acetic acid
  • Example 31 Preparation of l-(4-((4-(l-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2,2,2-trifluoroethyl)-lH- pyrazol- 1 -yl)methyl)benzyl)pyridin-2( 1 H)-one
  • reaction mixture was stirred for 1 h, quenched with saturated aqueous NH4CI, extracted with EA.

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Abstract

The invention provides compounds of Formula I and Formula II: A-B-C-D-E-F-G-J (I) C-D-E-F-G-J (II) wherein A, B, C, D, E, F, G, and J have any of the values defined in the specification, and salts thereof. The compounds are useful for inhibiting plasma kallikrein, and for treating a disease or condition in an animal where inhibition of plasma kallikrein is indicated.

Description

THERAPEUTIC INHIBITORY COMPOUNDS
Related Applications
This application claims priority under 35 U.S.C. § 119(e) to U.S. Provisional Patent Application Nos. 61/921,995, filed December 30, 2013 and 62/025,203, filed July 16, 2014, which applications are incorporated herein by reference.
Background of the Invention
Modulation of vascular permeability is important in regulating the passage of small molecules or blood cells between blood vessels and surrounding tissues. Vascular permeability can vary with the physiological states of tissues such as during inflammation, changes in blood pressure, and fluctuations in ion and nutrient gradients. The junctions between the endothelial cells that line blood vessels are the immediate controllers of vascular permeability. The strength of these junctions is tightly regulated by the kinin-kallikrein system of polypeptides and enzymes. Abnormalities in the kinin-kallikrein system can lead to a range of pathologies including angioedema, macular edema and brain edema.
The kinin-kallikrein system ("contact system") is responsible for the production of the peptide bradykinin, the proximal molecular mediator of increased vascular permeability (Busse PJ and Buckland MS, Clin. Exp. Allergy, 43(4), 385-94, 2013), blood vessel dilation, inflammation (Bjorkqvist J et al., Thromb. Haemost. 110(3), 399-407, 2013), and pain (Petho G and Reeh PW, Physiol. Rev., 92(4), 1699-775, 2012). The pathway activation begins with initial generation of zymogen XII (FXII), which is immediately converted into coagulation factor Xlla (FXIIa). FXIIa catalyzes the conversion of prekallikrein to kallikrein, the activated form, which is expressed primarily by hepatocytes in the liver. Activated kallikrein then acts via a positive feedback mechanism to catalyze further conversion of FXII to FXIIa, a process that results in sufficient kallikrein production to drive downstream processes. Kallikrein is a trypsin-like serine protease enzyme. Serine proteases cleave peptide bonds in proteins through a mechanism that involves the binding of a serine residue with the target molecule at the cleavage site. Kallikrein cleaves high molecular weight kinogen (HK) to produce bradykinin. Bradykinin then binds to the bradykinin 2R receptors (BK2R) on endothelial cells to trigger an increase in vascular permeability.
Angioedema is a potentially fatal blood disorder characterized by swelling that may occur in the face, gastrointestinal tract, extremities, genitals and upper airways. Angioedema attacks begin in the deeper layers of the skin and mucous membranes with localized blood vessel dilatation and increased permeability.
Symptoms of the disease result from the leakage of plasma from blood vessels into surrounding tissues.
Genetic ('hereditary') angioedema (ΉΑΕ') attacks result from unregulated activation of the kallikrein system with consequent overproduction of bradykinin and uncontrolled increases in vascular permeability. As vascular permeability rises beyond normal, plasma leaks out of the vasculature into surrounding tissue, causing swelling (Mehta D and Malik AB, Physiol. Rev., 86 (1), 279-367, 2006; Sandoval R et al., J. Physiol, 533(pt 2), 433-45, 2001 ; Kaplan AP and Greaves MW, Angioedema. J. Am. Acad. Dermatol, 2005).
HAE results from mutations in the genes that code for elements of the coagulation and inflammation pathways. The three forms of HAE are distinguished by their underlying causes and levels of the CI -esterase inhibitor (Cl-INH, serpin peptidase inhibitor, clade G, member 1) protein in the blood, which inhibits the activity of plasma kallikrein. In type I, patients have insufficient levels of functional Cl-INH, while type II patients have dysfunctional Cl-INH. While type I and II affect men and women at equal rates, type III, which primarily affects women, results from a mutation in coagulation factor XII (Hageman factor; HAE-FXII). The underlying causes of type I and II HAE are autosomal dominant mutations in Cl-INH gene (SERPING1 gene) on chromosome 11 (1 lql2-ql 3.1).
CIINH accounts for 90% of inhibition of FXIIa and 50% of inhibition of plasma kallikrein (Pixley RA et al., J. Biol. Chem., 260, 1723-9, 1985; Schapira M et al., Biochemistry, 20, 2738-43, 1981). In addition, Cl-INH also inactivates prekallikrein (Colman RW et al, Blood, 65, 311-8, 1985). When Cl-INH levels are normal, its activity blocks FXIIa from converting pre -kallikrein to kallikrein and blocks kallikrein's conversion to HK, thus preventing the production of bradykinin and the edemic episodes. When Cl-INH levels are low, or levels of dysfunction Cl-INH high, this inhibition fails and the pathogenic process ensues.
In addition to HAE, plasma kallikrein may contribute to non-hereditary angioedema, high altitude cerebral edema, cytotoxic cerebral edema, osmotic cerebral edema, diabetic macular edema (DME), clinically significant macular edema, cystoid macular edema (CME, Gao BB, Nat Med., 13(2), 181-8, 2007), retinal edema, radiation induced edema, lymph edema, glioma-associated edema, allergic edema e.g. airflow obstruction in chronic allergic sinusitis or perennial rhinitis. Other disorders of the plasma kallikrein system include retinopathy and diabetic retinopathy (Liu J and Feener EP, Biol. Chem. 394(3), 319-28, 2013), proliferative and non-pro liferative retinopathy (Liu J et al, Invest. Ophthalmol. Vis. Sci., 54(2), 2013), CME following cataract extraction, CME induced by cryotherapy, CME induced by uveitis, CME following vascular occlusion (e.g., central retinal vein occlusion, branch retinal vein occlusion or hemiretinal vein occlusion), complications related to cataract surgery in diabetic retinopathy, hypertensive retinopathy (JA Phillips et al., Hypertension, 53, 175-181, 2009), retinal trauma, dry and wet age-related macular degeneration (AMD), ischemic reperfusion injuries (C Storoni et al., JPET, 381, 849-954, 2006), e.g., in a variety of contexts associated with tissue and/or organ transplantation.
In addition, kinin-kallikrein system may contribute to focal cerebral ischemia, damage in surgically- induced brain injury, global cerebral ischemia, spinal cord injury (Radulovic M et al., J. Neuropathol. Exp. Neurol., 72(11), 1072-89, 2013), pain, ischemia, neurological and cognitive deficits, deep vein thrombosis (Bird JE et al., Thromb. Haemost. 2012 Jun; 107(6):1141-50), stroke, myocardial infarction (Konings J et al., Thromb. Res., 132(1), 138-42, 2013), acquired angioedema drug-related (ACE-inhibitors), obstructive hydrocephalus, traumatic brain injury, hemorrhagic stroke (e.g., cerebral stroke or subarachnoid stroke), intracerebral hemorrhage, hemorrhagic transformation of ischemic stroke, cerebral trauma associate with injury or surgery, brain aneurysm, arterio-venous malformation, reduction of blood losses during surgical procedures (e.g., cardiothoracic surgery, such as cardiopulmonary bypass or coronary artery bypass grafting), blood coagulation disorders such as thrombosis, itch, disorders with an inflammation component (such as multiple sclerosis; Ma D et al., Blood 2013), epilepsy (Simoes PS et al., Neurochem. Int., 58(4), 477-82, 2011), encephalitis, Alzheimer's disease (Shropshire TD, et al., Biol. Chem. 2013), excessive daytime sleepiness, essential hypertension, increased blood pressure associated with diabetes or hyperlipidemia, renal insufficiency (Martinez-Morillo E, Diamandis A, Diamandis EP, Clin. Chem. Lab. Med. 2012 Mar 24;50(5):931-4), chronic kidney disease, heart failure, disorders associated with increased vascular permeability (e.g., increased retinal vascular permeability, increased leg, feet, ankle vascular permeability), cerebral hemorrhage, microalbuminuria (Bodin S et al., Kidney Int. 2009 Aug;76(4):395-403), albuminuria and proteinuria, deep vein thrombosis, coagulation from post fibrinolytic treatments, angina, sepsis, arthritis (e.g., rheumatoid arthritis, osteoarthritis, infection arthritis; Dai J et al., Arthritis Rheum. 64(11), 3574-82, 2012), lupus, gout, psoriasis, blood loss during cardiopulmonary bypass, inflammatory bowel, diabetes (Feener EP et al., Thromb. Haemost., 110(3), 434-41, 2013), diabetic complications, infectious diseases, astrocyte-activation related diseases (e.g., Alzheimer's disease or multiple sclerosis; Burda JE, Glia., 61(9), 1456-70, 2013), Parkinson's disease, amyotrophic lateral sclerosis, Creutzfeld- Jacob disease, stroke, epilepsy and trauma (e.g., brain trauma), airflow obstruction in acute asthma; serositis associated with systemic lupus erythematosus (SLE) and other diseases.
Current treatments for angioedema, and those under development, target different elements in the HAE pathway. Three classes of therapies are currently available: (a) replacement therapies, (b) selective kallikrein inhibitors and (c) bradykinin receptors antagonists. (A) Replacement therapies have proven useful for both acute attacks, including emergency situations, such as laryngeal edema (Bork K et al., Transfusion, 45, 1774-1784, 2005; Bork K and Barnstedt S E, Arch. Intern. Med., 161, 714-718, 2001) and prophylaxis. (B) Selective Cl-INH inhibitors inactivate both a-FXIIa and β-FXIIa molecules active early in the HAE pathway that catalyze the production of kallikrein (Muller F and Renne T, Curr. Opin. HematoL, 15, 516-21, 2008; Cugno M et al., Trends Mol. Med. 15(2):69-78, 2009). (C) By blocking the bradykinin receptor antagonists prevent bradykinin from activating the vascular permeability pathway and stop the initiation of swelling.
In addition to HAE, plasma kallikrein inhibitors are considered to be useful in the treatment of other edemas such as macular edema and brain edema, and retinopathy, e.g., retinopathy associated with diabetes and/or hypertension. There is evidence that plasma kallikrein inhibitors may also be effective in the treatment of edema formation in diseases, e.g., edema formation related to ischemic reperfusion injuries.
The identification of effective small compounds which potently and specifically inhibit plasma kallikrein is therefore desirable. Preferred compounds should have little affinity for other proteases. They should be well absorbed from the gastrointestinal tract, be sufficiently metabolically stable and possess favorable pharmacokinetic properties. They should be non-toxic and demonstrate few side-effects.
Furthermore, the ideal drug candidate will be able to exist in a physical form that is stable, non-hygroscopic and easily formulated.
Design of small molecule drugs targeting plasma kallikrein may yield identification of compounds which target serine proteases similar to kallikrein such as Factor XIa, Factor Xlla, Factor Vila and related proteases also involved in blood coagulation cascades and vascular permeability pathways.
Currently there is a need for agents that are useful for inhibiting plasma kallikrein as well as Factor
XIa, Factor Xlla, or Factor Vila, or other proteases involved in blood coagulation cascades and vascular permeability pathways. Summary of the Invention
The invention provides a compound of the invention which is a compound of Formula I:
A-B-C-D-E-F-G-J (I)
wherein:
A is a 5 or 6 membered heterocyclic ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb;
B is absent or is (Ci-C3)alkyl;
C is phenyl or a 6-membered heteroaryl ring, which phenyl or 6-membered heteroaryl ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
D is absent, (Ci-C3)alkyl, halo(Ci-C3)alkyl, O, S, S(=0)2, or NRa;
E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
F is -S(=0)2NRa- when E is a 5-membered heteroaryl ring; or F is -C(=0)-NRa- and C is an unsubstituted phenyl when E is a 5-membered heteroaryl ring; or F is -C(=0)-NRa- or -S(=0)2NRa- when E has any value other than a 5-membered heteroaryl ring;
G is absent or is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, (Ci-C6)alkoxycarbonyl, and -C(=0)NRaRb;
J is aryl or heteroaryl, which aryl or heteroaryl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, - C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, - C(=0)NRaRb, or -C(=NRa)NRaRb;
Ra is H, (Ci-C6)alkyl, or (Ci-C6)alkanolyl; and
Rb is H, (Ci-C6)alkyl, or (Ci-C6)alkanolyl;
or a salt thereof. The invention also provides a compound of the invention which is a compound of Formula II:
C-D-E-F-G-J (II)
wherein:
C is a bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb;
D is absent, (Ci-C3)alkyl, halo(Ci-C3)alkyl, O, S, S(=0)2, or NRa;
E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
F is -S(=0)2NRa-, -NRa S(=0)2-, -NRa-C(=0)-, or -C(=0)-NRa-;
G is absent or is (Ci-C3)alkyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
J is bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, - C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, - C(=0)NRaRb, or -C(=NRa)NRaRb;
each Ra is independently H, (Ci-C6)alkyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or -C(=0)NRcRd, wherein each (Ci-C6)alkyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or -C(=0)NRcRd is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy,
halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
each Rb is independently H or (Ci-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb;
each Rc is independently H or (Ci-Ce)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb; and
each Rd is independently H or (Ci-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb;
or a salt thereof.
The invention also provides a pharmaceutical composition comprising a compound of Formula I or
Formula II, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, diluent, or carrier.
The invention also provides a method of treating a disease or condition in an animal wherein inhibition of plasma kallikrein is indicated comprising administering a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, to the animal.
The invention also provides a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, for use in medical therapy.
The invention also provides a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, for the prophylactic or therapeutic treatment of a disease or condition wherein inhibition of plasma kallikrein is indicated.
The invention also provides the use of a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, to prepare a medicament for treatment of a disease or condition in an animal wherein inhibition of plasma kallikrein is indicated.
The invention also provides processes and intermediates disclosed herein that are useful for preparing a compound of Formula I or Formula II or a salt thereof.
Compounds of Formula I or Formula II and salts thereof are useful for inhibiting plasma kallikrein.
Detailed Description
The following definitions are included to provide a clear and consistent understanding of the specification and claims. As used herein, the recited terms have the following meanings. All other terms and phrases have their ordinary meanings as one of skill in the art would understand. Such meanings may be obtained by reference to technical dictionaries, such as Hawley 's Condensed Chemical Dictionary 14th Edition, by R.J. Lewis, John Wiley & Sons, New York, N.Y., 2001.
References in the specification to "one embodiment", "an embodiment", etc., indicate that the embodiment described may include a particular aspect, feature, structure, moiety, or characteristic, but not every embodiment necessarily includes that aspect, feature, structure, moiety, or characteristic. Moreover, such phrases may, but do not necessarily, refer to the same embodiment referred to in other portions of the specification. Further, when a particular aspect, feature, structure, moiety, or characteristic is described in connection with one embodiment, it is within the knowledge of one skilled in the art to affect or connect such aspect, feature, structure, moiety, or characteristic with other embodiments, whether or not explicitly described, and such combinations are envisaged and are part of the present invention.
The singular forms "a," "an," and "the" include plural reference unless the context clearly dictates otherwise. Thus, for example, a reference to "a compound" includes a plurality of such compounds, so that a compound X includes a plurality of compounds X. It is further noted that the claims may be drafted to exclude any element, such as any recited definition of a particular variable or other optional elements. As such, this statement is intended to serve as antecedent basis for the use of exclusive terminology, such as "solely", "only", "except", "but not", and the like, in connection with any element or definition described herein, and/or the recitation of claim elements or use of "negative" limitations.
The term "and/or" means any one of the items, any combination of the items, or all of the items with which this term is associated. The phrases "one or more" and "at least one" are readily understood by one of skill in the art, particularly when read in context of its usage. For example, the phrase "one or more" can mean one, two, three, four, five, six, ten, 100, or any upper limit approximately two or ten times higher than a recited lower limit or element (e.g., typically one or two). For example, one or more substituents on a phenyl ring refers to one to five, or one to four, for example if the phenyl ring is currently disubstituted.
The term "about" can refer to a variation of ± 5%, ± 10%, ± 20%, or ± 25% of the value specified. For example, "about 50" percent can in some embodiments carry a variation from 45 to 55 percent. For integer ranges, the term "about" can include one or two integers greater than and/or less than a recited integer at each end of the range. Unless indicated otherwise herein, the term "about" is intended to include values, e.g., weight percentages, proximate to the recited range that are equivalent in terms of the functionality of the individual ingredient, the composition, or the embodiment. The term about can also modify the end-points of a recited range as discuss above in this paragraph.
As will be understood by the skilled artisan, all numbers, including those expressing quantities of ingredients, properties such as molecular weight, reaction conditions, and so forth, are approximations and are understood as being optionally modified in all instances by the term "about." These values can vary depending upon the desired properties sought to be obtained by those skilled in the art utilizing the teachings of the descriptions herein. It is also understood that such values inherently contain variability necessarily resulting from the standard deviations found in their respective testing measurements.
One skilled in the art will also readily recognize that where members are grouped together in a common manner, such as in a Markush group, the invention encompasses not only the entire group listed as a whole, but each member of the group individually and all possible subgroups of the main group.
Additionally, for all purposes, the invention may encompass not only the main group, but also the main group absent one or more of the group members. The invention therefore envisages the explicit exclusion of any one or more of members of a recited group. Accordingly, provisos may apply to any of the disclosed categories or embodiments whereby any one or more of the recited elements, species, or embodiments, may be excluded from such categories or embodiments, for example, for use in an explicit negative limitation.
An "effective amount" refers to an amount effective to treat a disease, disorder, and/or condition, or to bring about a recited effect. For example, an effective amount can be an amount effective to reduce the progression or severity of the condition or symptoms being treated. Determination of a therapeutically effective amount is well within the capacity of persons skilled in the art. The term "effective amount" can include an amount of a compound described herein, or an amount of a combination of compounds described herein, e.g., that is effective to treat or prevent a disease or disorder, or to treat the symptoms of the disease or disorder, in a host. Thus, an "effective amount" generally means an amount that provides the desired effect.
The terms "treating", "treat" and "treatment" can include (i) preventing a disease, pathologic or medical condition from occurring (e.g., prophylaxis); (ii) inhibiting the disease, pathologic or medical condition or arresting its development; (iii) relieving the disease, pathologic or medical condition; and/or (iv) diminishing symptoms associated with the disease, pathologic or medical condition. Thus, the terms "treat", "treatment", and "treating" can extend to prophylaxis and/or can include prevent, prevention, preventing, lowering, stopping or reversing the progression or severity of the condition or symptoms being treated. As such, the term "treatment" can include medical, therapeutic, and/or prophylactic administration, as appropriate.
The terms "inhibit", "inhibiting", and "inhibition" refer to the slowing, halting, or reversing the growth or progression of a disease, infection, condition, or activity of an enzyme. The inhibition can be greater than about 20%, 40%, 60%, 80%, 90%, 95%, or 99%, for example, compared to the growth or progression that occurs in the absence of the treatment or contacting.
Recursive Substituents. As to any of the formulas described herein or to the groups that contain one or more substituents, it is understood, of course, that such groups do not contain any substitution or substitution patterns that are sterically impractical and/or synthetically non-feasible. Also, the compounds of this invention include all stereochemical isomers arising from the substitution of these compounds.
Selected substituents of the compounds described herein may be present to a recursive degree. In this context, "recursive substituent" means that a substituent may recite another instance of itself. Because of the recursive nature of such substituents, theoretically, a large number may be present in any given claim. One of ordinary skill in the art of medicinal chemistry and organic chemistry understands that the total number of such substituents is reasonably limited by the desired properties of the compound intended. Such properties include, by of example and not limitation, physical properties such as molecular weight, solubility or log P, application properties such as activity against the intended target, and practical properties such as ease of synthesis.
Recursive substituents are an intended aspect of the invention. One of ordinary skill in the art of medicinal and organic chemistry understands the versatility of such substituents. To the degree that recursive substituents are present in a claim of the invention, the total number will be determined as set forth above or as described in this paragraph. In some embodiments, recursive substituents are present only to the extent that the molecular mass of the compound is about 400 to about 1600, about 450 to about 1200, about 500 to about 100, about 500 to about 800. In other embodiments, recursive substituents are present only to the extent that the molecular mass of the compound is less than 2000, less than 1800, less than 1600, less than 1500, less than 1400, less than 1200, less than 1000, less than 900, less than 800, less than 750, less than 700, or less than about 600. The term "carbocyclyl" used alone or as part of a larger moiety, refers to a saturated, partially unsaturated, or aryl ring system having 3 to 20 carbon atoms. In one embodiment, carbocyclyl includes 3 to 12 carbon atoms (C3-C12). In another embodiment, carbocyclyl includes C3-C8, C3-Cio or C5-C10. In other embodiment, carbocyclyl, as a monocycle, includes C3-C8, C3-C6 or C5-C6. In another embodiment, carbocyclyl, as a bicycle, includes C7-C12. In another embodiment, carbocyclyl, as a spiro system, includes C5-C12. Examples of monocyclic carbocyclyls include cyclopropyl, cyclobutyl, cyclopentyl, 1-cyclopent-l- enyl, 1 -cyclopent-2-enyl, l-cyclopent-3-enyl, cyclohexyl, perdeuteriocyclohexyl, 1-cyclohex-l -enyl, 1- cyclohex-2-enyl, l -cyclohex-3-enyl, cyclohexadienyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, cycloundecyl, phenyl, and cyclododecyl; bicyclic carbocyclyls having 7 to 12 ring atoms include [4,3], [4,4], [4,5], [5,5], [5,6] or [6,6] ring systems, for example bicyclo[2.2.1]heptane, bicyclo[2.2.2]octane, naphthalene, and bicyclo[3.2.2]nonane; and spiro carbocyclyls include spiro[2.2]pentane, spiro[2.3]hexane,
spiro[2.4]heptane, spiro[2.5]octane and spiro[4.5]decane. The term carbocyclyl includes aryl ring systems as defined herein. The term carbocycyl also includes cycloalkyl rings (e.g. saturated or partially unsaturated mono-, bi-, or spiro- carbocycles).
The term "alkyl," as used herein, refers to a saturated linear or branched-chain hydrocarbon radical.
A radical as used herein can be a monoradical, diradical, or multiradical, depending on how many substituents the group includes. In one embodiment, the alkyl radical is one to eighteen carbon atoms (Ci-Cis). In other embodiments, the alkyl radical is G-C12, C1-G0, Ci-Cs, Ci-Ce, C1-C5, C1-C4 or C1-C3. Examples of alkyl groups include methyl (Me, -CH3), ethyl (Et, -CH2CH3), 1 -propyl (n-Pr, n-propyl, -CH2CH2CH3), 2-propyl (i- Pr, i-propyl, -CH(CH3)2), 1 -butyl (n-Bu, n-butyl, -CH2CH2CH2CH3), 2-methyl-l -propyl (i-Bu, i-butyl, -
CH2CH(CH3)2), 2-butyl (s-Bu, s-butyl, -CH(CH3)CH2CH3), 2-methyl-2-propyl (t-Bu, t-butyl, -C(CH3)3), 1- pentyl (n-pentyl, -CH2CH2CH2CH2CH3), 2-pentyl (-CH(CH3)CH2CH2CH3), 3-pentyl (-CH(CH2CH3)2), 2- methyl-2-butyl (-C(CH3)2CH2CH3), 3-methyl-2 -butyl (-CH(CH3)CH(CH3)2), 3 -methyl- 1 -butyl
(-CH2CH2CH(CH3)2), 2-methyl-l -butyl (-CH2CH(CH3)CH2CH3), 1 -hexyl (-CH2CH2CH2CH2CH2CH3), 2- hexyl (-CH(CH3)CH2CH2CH2CH3), 3-hexyl (-CH(CH2CH3)(CH2CH2CH3)), 2-methyl-2-pentyl (- C(CH3)2CH2CH2CH3), 3-methyl-2-pentyl (-CH(CH3)CH(CH3)CH2CH3), 4-methyl-2-pentyl
(-CH(CH3)CH2CH(CH3)2), 3-methyl-3-pentyl (-C(CH3)(CH2CH3)2), 2-methyl-3-pentyl (- CH(CH2CH3)CH(CH3)2), 2,3-dimethyl-2-butyl (-C(CH3)2CH(CH3)2), 3,3-dimethyl-2-butyl (- CH(CH3)C(CH3)3, heptyl, octyl, nonyl, decyl, undecyl and dodecyl.
The term "alkenyl," as used herein, denotes a linear or branched-chain hydrocarbon radical with at least one carbon-carbon double bond. An alkenyl includes radicals having "cis" and "trans" orientations, or alternatively, "E" and "Z" orientations. In one example, the alkenyl radical is two to eighteen carbon atoms (C2-C10). In other examples, the alkenyl radical is C2-C8, C2-C6 or C2-C3. Examples include, but are not limited to, ethenyl or vinyl (-CH=CH2), prop-l -enyl (-CH=CHCH3), prop-2-enyl (-CH2CH=CH2),
2-methylprop-l -enyl, but-l-enyl, but-2-enyl, but-3-enyl, buta-l,3-dienyl, 2-methylbuta-l,3-diene, hex-l-enyl, hex-2-enyl, hex-3-enyl, hex-4-enyl and hexa-l,3-dienyl.
The term "alkynyl," as used herein, refers to a linear or branched hydrocarbon radical with at least one carbon-carbon triple bond. In one example, the alkynyl radical is two to eighteen carbon atoms (C2-C10). In other examples, the alkynyl radical is C2-C8, C2-C6 or C2-C3. Examples include, but are not limited to, ethynyl (-OCH), prop-l-ynyl (-OCCH3), prop-2-ynyl (propargyl, -CH2C≡CH), but-l-ynyl, but-2-ynyl and but-3- ynyl.
The term "alkoxy" refers to a linear or branched radical represented by the formula -OR in which R is alkyl, alkenyl, alkynyl or carbocycyl. Alkoxy groups include methoxy, ethoxy, propoxy, isopropoxy, and cyclopropoxy.
The term "haloalkyl," as used herein, refers to an alkyl as defined herein that is substituted with one or more (e.g. 1, 2, 3, or 4) halo groups.
The term "aryl" used alone or as part of a larger moiety as in "arylalkyl", "arylalkoxy", or
"aryloxyalkyl", refers to a monocyclic, bicyclic or tricyclic, carbon ring system, that includes fused rings, wherein at least one ring in the system is aromatic. The term "aryl" may be used interchangeably with the term "aryl ring". In one embodiment, aryl includes groups having 6-12 carbon atoms. In another embodiment, aryl includes groups having 6-10 carbon atoms. Examples of aryl groups include phenyl, naphthyl, anthracyl, phenanthrenyl, naphthacenyl, 1,2,3,4-tetrahydronaphthalenyl, lH-indenyl, 2,3-dihydro- lH-indenyl, and the like. A particular aryl is phenyl. In another embodiment aryl includes indanyl, naphthyl, and tetrahydronaphthyl, and the like, where the radical or point of attachment is on an aromatic ring.
The term "heteroaryl" used alone or as part of a larger moiety, e.g., "heteroarylalkyl", or
"heteroarylalkoxy", refers to a monocyclic, bicyclic or tricyclic ring system having 5 to 12 ring atoms, wherein at least one ring is aromatic and contains at least one heteroatom. In one embodiment, heteroaryl includes 4-6 membered monocyclic aromatic groups where one or more ring atoms is nitrogen, sulfur or oxygen. In another embodiment, heteroaryl includes 5-6 membered monocyclic aromatic groups where one or more ring atoms is nitrogen, sulfur or oxygen. Examples of heteroaryl groups include thienyl, furyl, imidazolyl, pyrazolyl, thiazolyl, isothiazolyl, oxazolyl, isoxazolyl, triazolyl, thiadiazolyl, oxadiazolyl, tetrazolyl, thiatriazolyl, oxatriazolyl, pyridyl, pyrimidyl, pyrazinyl, pyridazinyl, triazinyl, tetrazinyl, tetrazolo[l,5-b]pyridazinyl, imidazol[l,2-a]pyrimidinyl, purinyl, benzoxazolyl, benzofuryl, benzothiazolyl, benzothiadiazolyl, benzotriazolyl, benzoimidazolyl, indolyl, l,3-thiazol-2-yl, l,3,4-triazol-5-yl, l,3-oxazol-2- yl, l,3,4-oxadiazol-5-yl, l,2,4-oxadiazol-5-yl, l,3,4-thiadiazol-5-yl, lH-tetrazol-5-yl, l,2,3-triazol-5-yl, and pyrid-2-yl N-oxide. The terms "heteroaryl" also includes groups in which a heteroaryl is fused to one or more aryl, carbocyclyl, or heterocyclyl rings, where the radical or point of attachment is on the heteroaryl ring. Nonlimiting examples include indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H- quinolizinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl and pyrido[2,3-b]-l,4-oxazin-3(4H)-one. A heteroaryl group may be mono-, bi- or tri-cyclic.
As used herein, the term "heterocyclyl" refers to a "carbocyclyl" as defined herein, wherein one or more (e.g. 1, 2, 3, or 4) carbon atoms have been replaced with a heteroatom (e.g. O, N, or S). In some embodiments, a heterocyclyl refers to a saturated ring system, such as a 3 to 12 membered saturated heterocyclyl ring system. In some embodiments, a heterocyclyl refers to a heteroaryl ring system, such as a 5 to 14 membered heteroaryl ring system. The term heterocyclyl also includes Cs-Csheterocycloalkyl, which is a saturated or partially unsaturated mono-, bi-, or spiro-ring system comprising 3-8 carbons and one or more (1, 2, 3, or 4) heteroatoms.
In one example, heterocyclyl includes 3-12 ring atoms and includes monocycles, bicycles, tricycles and spiro ring systems, wherein the ring atoms are carbon, and one to five ring atoms is a heteroatom selected from nitrogen, sulfur or oxygen, which is independently optionally substituted by one or more groups. In one example, heterocyclyl includes 1 to 4 heteroatoms. In another example, heterocyclyl includes 3- to 7- membered monocycles having one or more heteroatoms selected from nitrogen, sulfur or oxygen. In another example, heterocyclyl includes 4- to 6-membered monocycles having one or more heteroatoms selected from nitrogen, sulfur or oxygen. In another example, heterocyclyl includes 3-membered monocycles. In another example, heterocyclyl includes 4-membered monocycles. In another example, heterocyclyl includes 5-6 membered monocycles. In one example, the heterocyclyl group includes 0 to 3 double bonds. Any nitrogen or sulfur heteroatom may optionally be oxidized (e.g. NO, SO, SO2), and any nitrogen heteroatom may optionally be quaternized (e.g. [NRi]+Cl", [NRi]+OH"). Example heterocyclyls include oxiranyl, aziridinyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, 1 ,2-dithietanyl, 1,3-dithietanyl, pyrrolidinyl, dihydro-lH-pyrrolyl, dihydrofuranyl, tetrahydrofuranyl, dihydrothienyl, tetrahydrothienyl, imidazolidinyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, 1 , 1 -dioxo-thiomorpholinyl, dihydropyranyl, tetrahydropyranyl,
hexahydrothiopyranyl, hexahydropyrimidinyl, oxazinanyl, thiazinanyl, thioxanyl, homopiperazinyl, homopiperidinyl, azepanyl, oxepanyl, thiepanyl, oxazepinyl, oxazepanyl, diazepanyl, 1 ,4-diazepanyl, diazepinyl, thiazepinyl, thiazepanyl, tetrahydrothiopyranyl, oxazolidinyl, thiazolidinyl, isothiazolidinyl, 1,1- dioxoisothiazolidinonyl, oxazolidinonyl, imidazolidinonyl, 4,5,6,7-tetrahydro[2H]indazolyl,
tetrahydrobenzoimidazolyl, 4,5,6,7-tetrahydrobenzo[d]imidazolyl, l,6-dihydroimidazol[4,5-d]pyrrolo[2,3- b]pyridinyl, thiazinyl, oxazinyl, thiadiazinyl, oxadiazinyl, dithiazinyl, dioxazinyl, oxathiazinyl, thiatriazinyl, oxatriazinyl, dithiadiazinyl, imidazolinyl, dihydropyrimidyl, tetrahydropyrimidyl, 1 -pyrrolinyl, 2-pyrrolinyl, 3 -pyrrolinyl, indolinyl, thiapyranyl, 2H-pyranyl, 4H-pyranyl, dioxanyl, 1,3-dioxolanyl, pyrazolinyl, pyrazolidinyl, dithianyl, dithiolanyl, pyrimidinonyl, pyrimidindionyl, pyrimidin-2,4-dionyl, piperazinonyl, piperazindionyl, pyrazolidinylimidazolinyl, 3-azabicyclo[3.1.0]hexanyl, 3,6-diazabicyclo[3.1. l]heptanyl, 6-azabicyclo[3.1.1 ]heptanyl, 3-azabicyclo[3.1.1 ]heptanyl, 3-azabicyclo[4.1.0]heptanyl,
azabicyclo[2.2.2]hexanyl, 2-azabicyclo[3.2.1]octanyl, 8-azabicyclo[3.2.1]octanyl, 2-azabicyclo[2.2.2]octanyl, 8-azabicyclo[2.2.2]octanyl, 7-oxabicyclo[2.2.1]heptane, azaspiro[3.5]nonanyl, azaspiro[2.5]octanyl, azaspiro[4.5]decanyl, l-azaspiro[4.5]decan-2-only, azaspiro[5.5]undecanyl, tetrahydroindolyl,
octahydroindolyl, tetrahydroisoindolyl, tetrahydroindazolyl, 1,1-dioxohexahydrothiopyranyl and 2- oxopyridin-l(2H)-yl. Examples of 5-membered heterocyclyls containing a sulfur or oxygen atom and one to three nitrogen atoms are thiazolyl, including thiazol-2-yl and thiazol-2-yl N-oxide, thiadiazolyl, including l,3,4-thiadiazol-5-yl and l,2,4-thiadiazol-5-yl, oxazolyl, for example oxazol-2-yl, and oxadiazolyl, such as l,3,4-oxadiazol-5-yl, and l,2,4-oxadiazol-5-yl. Examples of 5-membered ring heterocyclyls containing 2 to 4 nitrogen atoms include imidazolyl, such as imidazol-2-yl; triazolyl, such as l,3,4-triazol-5-yl; l,2,3-triazol-5- yl, l,2,4-triazol-5-yl, and tetrazolyl, such as lH-tetrazol-5-yl. Example benzo-fused 5-membered heterocyclyls are benzoxazol-2-yl, benzthiazol-2-yl and benzimidazol-2-yl. Examples of 6-membered heterocyclyls contain one to three nitrogen atoms and optionally a sulfur or oxygen atom, for example pyridyl, such as pyrid-2-yl, pyrid-3-yl, and pyrid-4-yl; pyrimidyl, such as pyrimid-2-yl and pyrimid-4-yl; triazinyl, such as l,3,4-triazin-2-yl and l,3,5-triazin-4-yl; pyridazinyl, in particular pyridazin-3-yl, and pyrazinyl. The pyridine N-oxides and pyridazine N-oxides and the pyridyl, pyrimid-2-yl, pyrimid-4-yl, pyridazinyl and the l,3,4-triazin-2-yl groups, are other example heterocyclyl groups.
As used herein, the term "partially unsaturated" refers to a ring moiety that includes at least one double or triple bond between ring atoms but the ring moiety is not aromatic.
It will be appreciated by those skilled in the art that compounds of the invention having a chiral center may exist in and be isolated in optically active and racemic forms. Some compounds may exhibit polymorphism. It is to be understood that the present invention encompasses any racemic, optically-active, polymorphic, or stereoisomeric form, or mixtures thereof, of a compound of the invention, which possess the useful properties described herein, it being well known in the art how to prepare optically active forms (for example, by resolution of the racemic form by recrystallization techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary phase.
When a bond in a compound formula herein is drawn in a non-stereochemical manner (e.g. flat), the atom to which the bond is attached includes all stereochemical possibilities. When a bond in a compound formula herein is drawn in a defined stereochemical manner (e.g. bold, bold-wedge, dashed or dashed-wedge), it is to be understood that the atom to which the stereochemical bond is attached is enriched in the absolute stereoisomer depicted unless otherwise noted. In one embodiment, the compound may be at least 51% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 60% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 80%> the absolute stereoisomer depicted. In another embodiment, the compound may be at least 90% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 95 the absolute stereoisomer depicted. In another embodiment, the compound may be at least 99% the absolute stereoisomer depicted.
Specific values listed below for radicals, substituents, and ranges, are for illustration only; they do not exclude other defined values or other values within defined ranges for the radicals and substituents.
Specifically, (Ci-Ce)alkyl can be methyl, ethyl, propyl, isopropyl, butyl, iso-butyl, sec-butyl, pentyl, 3-pentyl, or hexyl; (C3-C6)cycloalkyl can be cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl; (C3- C6)cycloalkyl(Ci-C6)alkyl can be cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl,
cyclohexylmethyl, 2-cyclopropylethyl, 2-cyclobutylethyl, 2-cyclopentylethyl, or 2-cyclohexylethyl; (Ci- Ce)alkoxy can be methoxy, ethoxy, propoxy, isopropoxy, butoxy, iso-butoxy, sec-butoxy, pentoxy, 3-pentoxy, or hexyloxy; (C2-Ce)alkenyl can be vinyl, allyl, 1-propenyl, 2-propenyl, 1-butenyl, 2-butenyl, 3-butenyl, 1,- pentenyl, 2-pentenyl, 3-pentenyl, 4-pentenyl, 1 - hexenyl, 2-hexenyl, 3-hexenyl, 4-hexenyl, or 5-hexenyl; (C2- C6)alkynyl can be ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 3-butynyl, 1 -pentynyl, 2-pentynyl, 3-pentynyl, 4-pentynyl, 1- hexynyl, 2-hexynyl, 3-hexynyl, 4-hexynyl, or 5-hexynyl; (Ci-C6)alkanoyl can be acetyl, propanoyl or butanoyl; halo(Ci-C6)alkyl can be iodomethyl, bromomethyl, chloromethyl,
fluoromethyl, trifluoromethyl, 2-chloroethyl, 2-fluoroethyl, 2,2,2-trifluoroethyl, or pentafluoroethyl; halo(Ci- Ce)alkoxy can be fluoromethoxy, trifluoromethoxy, 2-fluoroethoxy, 2-chloroethoxy, 2,2,2-trifluoroethoxy, or pentafluoroethoxy; hydroxy(Ci-C6)alkyl can be hydroxymethyl, 1 -hydroxy ethyl, 2-hydroxyethyl, 1 - hydroxypropyl, 2-hydroxypropyl, 3-hydroxypropyl, 1 -hydroxybutyl, 4-hydroxybutyl, 1 -hydroxypentyl, 5- hydroxypentyl, 1 -hydroxyhexyl, or 6-hydroxyhexyl; (Ci-C6)alkoxycarbonyl can be methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, pentoxycarbonyl, or
hexyloxycarbonyl; (C2-Ce)alkanoyloxy can be acetoxy, propanoyloxy, butanoyloxy, isobutanoyloxy, pentanoyloxy, or hexanoyloxy; aryl can be phenyl, indenyl, or naphthyl; and heteroaryl can be furyl, imidazolyl, triazolyl, triazinyl, oxazoyl, isoxazoyl, thiazolyl, isothiazoyl, pyrazolyl, pyrrolyl, pyrazinyl, tetrazolyl, pyridyl, (or its N-oxide), thienyl, pyrimidinyl (or its N-oxide), indolyl, isoquinolyl (or its N-oxide) or quinolyl (or its N-oxide).
In an aspect of the invention there is provided a compound of Formula I:
A-B-C-D-E-F-G-J (I)
wherein:
A is a 5 or 6 membered heterocyclic ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
B is absent or is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci- C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(G- C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
C is phenyl or heteroaryl, which phenyl or heteroaryl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, (Ci- C6)alkyl-NRaRb, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
D is absent, (Ci-C3)alkyl, halo(Ci-C3)alkyl, hydroxy(Ci-C3)alkyl, alkoxy(Ci-C3)alkyl, O, S, S(=0)2, -
CH(NRaRb)-, or NRa;
E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
F is -S(=0)2NRa-, -NRaS(=0)2-, or -NRa-C(=0)- when E is a 5-membered heteroaryl ring; or F is -C(=0)-NRa- and C is an unsubstituted phenyl when E is a 5-membered heteroaryl ring; or F is -S(=0)2NRa-, -NRaS(=0)2-, -NRa-C(=0)-, -C(COORa)-, -C(CF3)- or -C(=0)-NRa-; when E has any value other than a 5-membered heteroaryl ring;
G is absent or is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(G- C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(G- C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
J is aryl or heteroaryl, which aryl or heteroaryl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3- C6)carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (G- Ce)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-
C6)alkanoyloxy, -C(=0)ORa, -NRaRb, -C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, -C(=0)NRaRb, or -C(=NRa)NRaRb; or J is -0-NH-C(NH)-NH2;
each Ra is independently H, (G-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (G-C6)alkylS(0)2- or -C(=0)NRcRd, wherein each (Ci-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or -
C(=0)NRcRd is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(G-C6)alkyl, (Ci-C6)alkoxy, halo(G- Ce)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(G-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rb is independently H or (G-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rc is independently H or (Ci-Ce)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (G-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rd is independently H or (G-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb; and
each Re is independently H or (Ci-Ce)alkyl;
or a salt thereof.
In an embodiment D is hydroxy(G-C6)alkyl. In an embodiment D is alkoxy(G-C3)alkyl.
In an embodiment F is -C(COORa)-. In an embodiment F is -C(CF3)-. In an embodiment J is -0-NH-C(NH)-NH2. In an embodiment J is -C(NH)-NH2.
In an embodiment, compounds of the invention have the Formula I-aa or I-bb:
-F-G-J
Figure imgf000016_0001
I-aa I-bb
wherein A, B, C, D and E are previously defined. In a particular embodiment, E is phenyl. In a particular embodiment, E is pyridinyl. In a particular embodiment, E is indazolyl. In a particular embodiment, E is piperidinyl. In a particular embodiment, E is pyrazinyl. In a particular embodiment, E is indolyl. In a particular embodiment, E is pyrrolidinyl. In a particular embodiment, E is piperazinyl. In a particular embodiment, E is pyrazolyl. In a particular embodiment, E is 5-oxo-4,5-dihydropyrazinyl.
In another embodiment, the compound of Formula I is
A-B-C-D-E-F-G-J (I)
wherein:
A is a 5 or 6 membered heterocyclic ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb;
B is absent or is (Ci-C3)alkyl;
C is phenyl or a 6-membered heteroaryl ring, which phenyl or 6-membered heteroaryl ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, (Ci-C6)alkyl-NRaRb, -NRaRb, and -C(=0)NRaRb;
D is absent, (Ci-C3)alkyl, halo(Ci-C3)alkyl, O, S, S(=0)2, -CH(NRaRb)-, or NRa;
E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
F is -S(=0)2NRa- when E is a 5-membered heteroaryl ring; or F is -C(=0)-NRa- and C is an unsubstituted phenyl when E is a 5-membered heteroaryl ring; or F is -C(=0)-NRa- or -S(=0)2NRa- when E has any value other than a 5-membered heteroaryl ring;
G is absent or is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, (Ci-C6)alkoxycarbonyl, and -C(=0)NRaRb;
J is aryl or heteroaryl, which aryl or heteroaryl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, - C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, - C(=0)NRaRb, or -C(=NRa)NRaRb;
Ra is H, (Ci-C6)alkyl, or (Ci-C6)alkanolyl; and
Rb is H, (Ci-C6)alkyl, or (Ci-C6)alkanolyl;
or a salt thereof.
A specific value for A is a piperidine, indazole, morpholine, piperazine, oxazolidine, imidazole, or pyrrolidine, that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci- Ce)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl,
(Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb.
A specific value for A is a piperidine, indazole, morpholine, piperazine, oxazolidine, imidazole, or pyrrolidine, that is substituted with oxo and that is optionally substituted with one or more groups
independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb.
A specific value for A is a 2-oxopiperidin-l-yl, pyrazole-l-yl, 3-oxomorpholino, 2-oxooxazolidin-3- yl, 2-oxoimidazolidin-l-yl, or 2-oxopyrrolidin-l -yl. In some embodiments, the heterocyclic ring of A is substituted with a fluoro group.
A specific value for B is C¾.
A specific value for C is phenyl or a 6-membered heteroaryl ring, which phenyl or 6-membered heteroaryl ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci- Ce)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl,
(Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb.
A specific value for C is pyridine, pyrimidine, pyrazine, pyridazine, or triazine, wherein any pyridine, pyrimidine, pyrazine, pyridazine, or triazine is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb.
A specific value for C is phenyl or pyridyl where any phenyl or pyridyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb.
A specific value for C is phenyl or pyridyl, where the phenyl or pyridyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, cyano, -NRaRb, and -C(=0)NRaRb.
A specific value for C is phenyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, cyano, -NRaRb, and -C(=0)NRaRb.
A specific value for C is pyridyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, cyano, -NRaRb, and -C(=0)NRaRb.
A specific value for C is phenyl, aminophenyl, fluorophenyl, chlorophenyl, cyanophenyl,
aminocarbonylphenyl, hydroxyphenyl, pyridyl, aminopyridyl, or methylsulfonylphenyl.
In another aspect of the invention, there is provided compound of Formula II:
C-D-E-F-G-J (II)
wherein:
C is a bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (G-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -(Ci-C6)alkyl-COOH, - (Ci-C6)alkyl-CO-(Ci-C6)alkoxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
or C is a monocyclic carbocyclic ring or a monocyclic heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, -(Ci-C6)alkyl-NRaRb, -(Ci-C6)alkyl-NH-C(0)-NRaRb, - (Ci-C6)alkyl-NH-C(NH)-NRaRb, -(Ci-C6)alkyl-CO-(Ci-C6)alkoxy, -(Ci-C6)alkyl-COOH, -(Ci-C6)alkyl-CO- NRaRb, -(Ci-C6)alkoxy-CO-NRaRb, S(=0)2Re, and -C(=0)NRaRb;
D is absent, (Ci-C3)alkyl, halo(Ci-C3)alkyl, O, S, S(=0)2, -CH(NRaRb)-, or NRa;
E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
F is -S(=0)2NRa-, -NRa S(=0)2-, -NRa-C(=0)-, or -C(=0)-NRa-;
G is absent or is (Ci-C3)alkyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
J is bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, - C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, - C(=0)NRaRb, or -C(=NRa)NRaRb;
or J is a monocyclic heterocyclic ring, which heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6)carbocyclyl(Ci-C6)alkyl, (G-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci- Ce)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, - (Ci-C6)alkyl-NRaRb, -(Ci-C6)alkyl-NH-C(0)-NRaRb, -(Ci-C6)alkyl-NH-C(NH)-NRaRb, -(Ci-C6)alkyl-CO-(Ci- C6)alkoxy, -(Ci-C6)alkyl-COOH, -(Ci-C6)alkyl-CO-NRaRb, -(Ci-C6)alkoxy-CO-NRaRb, S(=0)2Re, and - C(=0)NRaRb;
each Ra is independently H, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or -C(=0)NRcRd, wherein each (G-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or - C(=0)NRcRd is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(G-C6)alkyl, (Ci-C6)alkoxy, halo(G- Ce)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl,
(Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rb is independently H or (G-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (G-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
each Rc is independently H or (Ci-Ce)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (G-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb; and
each Rd is independently H or (G-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (G-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
each Re is independently H or (Ci-Ce)alkyl;
or a salt thereof.
In a particular embodiment, C is a monocyclic carbocyclic ring or a monocyclic heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(G- C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(G- C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, -(G-C6)alkyl-NRaRb, - (Ci-C6)alkyl-NH-C(0)-NRaRb, -(Ci-C6)alkyl-NH-C(NH)-NRaRb, -(Ci-C6)alkyl-CO-(Ci-C6)alkoxy, -(Ci- C6)alkyl-COOH, -(Ci-C6)alkyl-CO-NRaRb, -(Ci-C6)alkoxy-CO-NRaRb, S(=0)2Re, and -C(=0)NRaRb.
In a particular embodiment, C is pyridyl. In a particular embodiment, C is phenyl. In another particular embodiment, C is quinoline or quinoline substituted with halo, such as fluoro, or a (Ci-C6)alkyl, such as methyl.
In a particular embodiment, C is substituted with -(Ci-C6)alkyl-NRaRb. In a particular embodiment, C is substituted with aminomethyl. In a particular embodiment, C is substituted with -(Ci-C6)alkyl-NRaRb. In a particular embodiment, C is substituted with acetamidomethyl. In a particular embodiment, C is substituted with -(Ci-C6)alkyl-CO-NRaRb. In a particular embodiment, C is substituted with 2-amino-2-oxoethyl. In a particular embodiment, C is substituted with 3-amino-3-oxopropyl. In a particular embodiment, C is substituted with -(Ci-C6)alkyl-CO-(Ci-C6)alkoxy. In a particular embodiment, C is substituted with -CH2- COO-Me. In a particular embodiment, C is substituted with -(CH2)2-COO-Me. In a particular embodiment, C is substituted with -(Ci-C6)alkyl-NH-C(0)-NRaRb. In a particular embodiment, C is substituted with -CH2- NH-CO-NH2. In a particular embodiment, C is substituted with -(Ci-C6)alkoxy-CO-NRaRb. In a particular embodiment, C is substituted with -0-CH2-CO-NH2. In a particular embodiment, C is substituted with -(Ci- C6)alkyl-NRaRb. In a particular embodiment, C is substituted with methylacetamidomethyl. In a particular embodiment, C is substituted with 1 -acetamidoethyl.
In a particular embodiment, there is provided a compound of Formula Il-aa or Il-bb:
Figure imgf000020_0001
wherein C is as defined herein for Formula II. In a particular embodiment, C phenyl, optionally substituted as defined herein for Formula II. In another embodiment, C is pyridyl, optionally substituted as defined herein for Formula II.
In another embodiment, there is provided a compound of Formula II-cc through Il-nn:
Figure imgf000020_0002
(Il-ee)
Figure imgf000021_0001
Figure imgf000021_0002
wherein J is as defined herein for Formula II. In a particular embodiment, J is indole. In a particular embodiment, J is 3-chloro-6-fluoro-lH-indol-5-yl. In a particular embodiment, J is 3-chloro-lH-indol-5-yl. In a particular embodiment, J is indazolyl. In a particular embodiment, J is 5-chloro-lH-indazol-3-yl. In a particular embodiment, J is pyrrolo[2,3-b]pyridinyl. In a particular embodiment, J is 3-chloro-lH-pyrrolo [2,3-b]pyridin-5-yl. In a particular embodiment, J is a monocyclic heterocyclic ring. In a particular embodiment, J is an amino-dimethylpyridinyl group such as 6-amino-2,4-dimethylpyridin-3-yl.
In a particular embodiment, there is provided a compound of Formula II:
C-D-E-F-G-J (II)
wherein:
C is a bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb;
D is absent, (Ci-C3)alkyl, halo(G-C3)alkyl, O, S, S(=0)2, -CH(NRaRb)-, or NRa; E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
F is -S(=0)2NRa-, -NRaS(=0)2-, -NRa-C(=0)-, or -C(=0)-NRa-;
G is absent or is (Ci-C3)alkyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
J is bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, - C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, - C(=0)NRaRb, or -C(=NRa)NRaRb;
each Ra is independently H, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or -C(=0)NRcRd, wherein each (Ci-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or - C(=0)NRcRd is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci- Ce)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb;
each Rb is independently H or (Ci-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb;
each Rc is independently H or (Ci-Ce)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb; and
each Rd is independently H or (Ci-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb; or a salt thereof.
A specific compound of Formula II is a compound of Formula lib:
Figure imgf000023_0001
Formula lib
wherein each Xi, X2, X3 and X4 is independently CH or N; and G is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
A specific compound of F of Formula lie:
Figure imgf000023_0002
Formula lie
wherein each of Xi, X2, X3 and X4 is independently CH or N; and G is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
A specific compound of Formula I is a compound of Formula lb:
Figure imgf000023_0003
Formula lb
wherein each Xi, X2, X3 and X4 is independently CH or N; and G is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
A specific compound of Formula I is a compound of Formula Ic:
Figure imgf000023_0004
Formula Ic
wherein each of Xi, X2, X3 and X is independently CH or N; and G is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
A specific value for C is a quinoline that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6)
carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb.
A specific value for C is a 6-quinoline that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6)
carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb.
A specific value for C is a 2-methyl-6-quiolinyl.
A specific value for D is (Ci-C3)alkyl. Another specific value for D is methylene.
A specific value for E is a carbocyclic ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb.
A specific value for E is a heterocyclic ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and - C(=0)NRaRb;
A specific value for E is pyrazole, pyridine, indole, phenyl, morpholine, pyrimidine, pyrazine, pyradizine, 2-oxooxazolidine, pyrrolidine, 2-oxopyrrolidine, piperazine, oxopiperazine, oxopyridine, oxopyrimidine, oxopiperidine, oxomorpholine, azaspiro[3,4]octane, 5-thia-2-azaspiro[3,4]octane, hydroxypyridine, indazole, lH-pyrrolo[2,3-b]pyridine, or hydroxypyrimidine, wherein E is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb.
A specific value for E is pyrazole, pyridine, or phenyl.
A specific value for E is pyrazole.
A specific value for E is pyridine.
A specific value for F is -S(=0)2NRa-.
A specific value for F is -NRa S(=0)2-.
A specific value for F is -NRa-C(=0)-. A specific value for F is -C(=0)-NRa-.
A specific value for G is (Ci-C3)alkyl optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci- C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(G- C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb.
Another specific value for G is methylene.
A specific value for J is aryl. Another specific value for J is heteroaryl. The aryl or heteroaryl can be substituted. In one embodiment, the substituents are one or more halo, amino, or methyl groups, or a combination thereof.
Another specific value for J is 8-10 membered heteroaryl ring. The heteroaryl ring can be substituted, for example, with one or more halo, amino, or methyl groups, or a combination thereof.
Another specific value for J is monocyclic heterocyclic ring. In one embodiment, the monocyclic heterocyclic ring is substituted with one or more halo, amino, or methyl groups, or a combination thereof.
A specific value for J is indazole that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, -C(=0)NRaRb, - C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, -C(=0)NRaRb, or - C(=NRa)NRaRb.
A specific value for J is indazole-3-yl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb,-C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with NRaRb.
A specific value for J is chloro-indazole-3-yl. Another specific value for J is 5-chloro-indazole-3-yl.
Specific values for J also include indole, pyridine, azaindole, quinoline, or isoquinoline, each optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, -C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, -C(=0)NRaRb, or -C(=NRa)NRaRb.
Specific values for J further include 1 -aminoisoquinolin-6-yl, 3-chloro-6-fluoro-lH-indol-5-yl, 6- amino-2,4-dimethylpyridin-3-yl, 3-chloro-4-fluoro-lH-indol-5-yl, or 3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl, which groups can be optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb,-C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, NRaRb, or -C(=0)NRaRb. In one embodiment, J is isoquinoline. The isoquinoline can be optionally substituted as described above. In one specific embodiment, J is 1 -aminoisoquinolin-6-yl.
A specific value for Ra or Rb is H. Another specific value for Ra or Rb is acetyl. Another specific value for Ra or Rb is 2-hydroxy-acetyl. Another specific value for Ra or Rb is 2-amino-acetyl. Another specific value for Ra or Rb is methoxyformyl. Another specific value for Ra or Rb is cyclopropylformyl. Another specific value for Ra or Rb is cyclopropyl.
Specific values for Xi, X2, X3 and X4 are CH.
Specific values for Xi is N, and X2, X3 and X4 are CH.
Specific values for Xi and X3 are N, and X2 and X4 are CH.
Specific values for Xi and X4 are N, and X2 and X3 are CH.
Specific values for Xi and X2 are N, and X3 and X4 are CH.
A specific compound of the invention can be selected from Table 1.
Specific compound of the invention can also be selected from:
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridine-2-sulfonamide; N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)picolinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)picolinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)picolinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-indole-3- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-2- carboxamide;
methyl 2-(5-chloro-3-((l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4-carboxamido)methyl)- 1 H-indazol- 1 -yl)acetate;
2-(5-chloro-3-((l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4-carboxamido)methyl)-lH- indazol-l-yl)acetic acid;
N-((l-(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)- 1 H-pyrazole-4-carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)- benzenesulfonamide; N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)pyridine-3- sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(methyl(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)- benzenesulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)morpholine-2- carboxamide; N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrimi
carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrazine-2-carbo N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridine-4-sulfo
N-((5-chloro-lH-indazol-3-yl)methyl)-2-oxo-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)oxazolidine-5- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-l-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)- pyrrolidine-3- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-oxo-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyTO
carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)nicotinamide;
N-((5-chloro H-indazol-3-yl)methyl)-3-(methyl(4-((2-oxopyridin (2H)-yl)methyl)phenyl)- amino)b enzamide ;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(N-(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)acetamido)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(methyl(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)amino)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(N-(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)- acetamido)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(N-(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)- acetamido)picolinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridine-2-sulfo
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)pyridine-4- sulfonamide;
N-(3 -(N-((5-chloro-l H-indazol-3 -yl)methyl)sulfamoyl)phenyl)-N-(4-((2-oxopyridin- 1 (2H)- yl)methyl)phenyl)acetamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)pyridine-2- sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2^
sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrimidine-4- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrimidine-4- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrrolidine-l - carboxamide; N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrrolid^
sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-l-(4-((2-ox
carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-oxo-3-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)oxazolidine-5- sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-l- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)mo^holine-4- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-methyl-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l - carboxamide;
4-acetyl-N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-oxo-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l - carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-methyl-3-oxo-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)piperazine-l -carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-oxo-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)morpholine-2- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-l-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)piperidine- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-((4-((2-oxopyrid
carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-5-azaspiro[3.4]octane-2- carboxamide;
N-((5-chloro H-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-5 hia-2- azaspiro[3.4]octane-8-carboxamide 5,5-dioxide;
2-(5-chloro H-indazol-3-yl)-2-(l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)acetic acid;
methyl 2-(5-chloro-lH-indazol-3-yl)-2-(l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)acetate;
N-(2-amino- 1 -(5 -chloro- 1 H-indazol-3 -yl)-2-oxoethyl)- 1 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H- pyrazole-4-carboxamide; 3 -(5-chloro- 1 H-indazol-3 -yl)-3 -( 1 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H-pyrazole-4- carboxamido)propanoic acid;
methyl 3-(5-chloro-lH-indazol-3-yl)-3-(l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)propanoate;
N-(3 -amino- 1 -(5 -chloro- 1 H-indazol-3 -yl)-3 -oxopropyl)- 1 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H- pyrazole-4-carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-oxo-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-l,6- dihydropyridine-3-carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-oxo-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-l,2- dihydropyrimidine-5-carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-4,5- dihydropyrazine-2-carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)benzamide; and
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(difluoro(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzamide;
and salts thereof.
A specific compound can be further selected from:
N-(4-carbamimidoylbenzyl)-3 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)benzamide;
N-(4-(aminomethyl)benzyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-aminopyridin-3 -yl)methyl)-3 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)benzamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((5-chlorobenzo[b]thiophen-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chlorobenzo[b]thiophen-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloro-lH-indol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((7-chloronaphthalen-2-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloronaphthalen-2-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((l-fluoronaphthalen-2-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloro-l -fluoronaphthalen-2-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloroisoquinolin-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloroquinolin-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide; and
N-((2-chlorobenzo[b]thiophen-5-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
and salts thereof.
A specific compound can also be selected from:
N-(4-carbamimidoylbenzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-(4-(aminomethyl)benzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-aminopyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)- nicotinamide; N-((6-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((5-chlorobenzo[b]thiophen-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; N-((6-chlorobenzo[b]thiophen-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; N-((6-chloro-lH-indol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((7-chloronaphthalen-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-chloronaphthalen-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((l -fluoronaphthalen-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-chloro-l -fluoronaphthalen-2-yl)methyl)-5-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)- nicotinamide; N-((6-chloroisoquinolin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-chloroquinolin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; and
N-((2-chlorobenzo[b]thiophen-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; and salts thereof.
A specific compound can yet further be selected from:
3 -(4-(( 1 H-pyrazol- 1 -yl)methyl)benzyl)-N-((5 -chloro- 1 H-indazol-3 -yl)methyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopiperidin-l -yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((3-oxomorpholino)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopiperazin-l -yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxooxazolidin-3-yl)methyl)benzyl)benzamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(4-((2-oxoimidazolidin- 1 -yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyrrolidin-l-yl)methyl)benzyl)benzamide;
N-((5 -chloro- 1 H-indazol-3 -yl)methyl)-3-((2-methylquinolin-6-yl)methyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)benzamide; N-((5-chloro-lH-indazol-3-yl)methyl)-3-((5-((2-oxopyridin-l(2H)-yl)methyl)pyridin-2-yl)methyl)benzamide; 3-((6-amino-5-((2-oxopyridin-l(2H)-yl)methyl)pyridin-2-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)benzamide;
3-((2-amino-6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(2-chloro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(2-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
3-(2-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(2-hydroxy-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide; 3-(2-amino-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)benzamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(3 -fluoro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(3-cyano-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)benzamide; 3-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)benz
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(3-hydroxy-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)ben^
3-(3-amino-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)benzam and salts thereof.
A specific compound can also be selected from:
5-(4-(( 1 H-pyrazol- 1 -yl)methyl)benzyl)-N-((5-chloro- 1 H-indazol-3 -yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopiperidin-l -yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((3-oxomorpholino)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopiperazin-l -yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxooxazolidin-3-yl)methyl)benzyl)nicotinamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-5-(4-((2-oxoimidazolidin- 1 -yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyrrolidin-l-yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-((2-methylquinolin-6-yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-((5-((2-oxopyridin-l(2H)-yl)methyl)pyridin-2- yl)methyl)nicotinamide;
5-((6-amino-5-((2-oxopyridin-l(2H)-yl)methyl)pyridin-2-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide;
5-((2-amino-6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; 5-(2-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-hydroxy-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; 5-(2-amino-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)nicotinamide; N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; 5-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-hydroxy-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; 5-(3-amino-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)nicotinamide; and salts thereof.
A specific compound of the invention can also be selected from:
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide; N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-cyanoquinolin-3-yl)methyl)isonicotinamide; 3-((4-(((l-aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)quinoline-6-carboxamide; N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-chloroquinolin-3-yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-(hydroxymethyl)quinolin-3-yl)methyl)isonicotinam N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-(amm^
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinamide;
6-((4-(((l-aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)quinoline-3-carboxamide; N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-(hydroxymethyl)quinolin-6-yl)methyl)isonicotinam N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-(amm^
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6-yl)methyl)ison^ N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-meth^^
6-((4-(((l-aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)-3-methylquinoline-8-carb acid;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)m
yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6-yl)methyl)isonico
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridm^
yl)methyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-methyl-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)qum yl)methyl)isonicotinamide;
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)qum yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-fluo
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-fluoro-8-sulfamoylquinolin-6-yl)methyl)isonicotinami
6-((4-(((l -aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)-3-fluoroquinoline-8-carboxylic acid;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6 yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6-yl)methyl)isonicotm
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridm^
yl)methyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-methyl-2-(trifluoromethyl)pyridm^
yl)methyl)isonicotinamide;
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2-methyl-5-(trifluoromethyl)pyridm^
yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide; N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinami N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamid N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinami N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide; N-((6-fluoro- 1 H-indol-5-yl)methyl)-2-((3 -methylquinolin-6-yl)methyl)isonicotinamide;
N-((6-fluoro-lH-indazol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide; N-((3-chloro-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((5-chloro-lH-indol-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-6-methyl-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamid
N-((3-chloro-6-methyl-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide;
N-((3-chloro-lH-pyrrolo[3,2-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)ison^ N-((lH-pyrrolo[3,2-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide; N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinam N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinami
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinamid
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinami N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isoni^ N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide; N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-chloro-8-(methylsulfonyl)quinolin^ yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-chloro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-chloro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-chloro-8-(methylsulfonyl)quinolin-6-yl)methyl)isonicotm
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)ison^
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinamide;
N-((6-fluoro-lH-indol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((6-fluoro-lH-indazol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-lH-indol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide; N-((5-chloro-lH-indol-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamid^ N-((3 -chloro-6-methyl- 1 H-indol-5-yl)methyl)-2-((3 -fluoroquinolin-6-yl)methyl)isonicotinamide; N-((3-chloro-6-methyl H-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6- yl)methyl)isonicotinamide;
N-((3-chloro-lH-pyrrolo[3,2-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)i
N-((lH-pyrrolo[3,2-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinam
N-((lH-benzo[d]imidazol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((3-aminobenzo[d]isoxazol-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide; N-((3-amino-lH-indazol-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((2-aminobenzo[d]oxazol-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((2-aminobenzo[d]oxazol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide; N-((6-amino-4-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-(3-(me lsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((3-chloro H-indol-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin-6- yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methyl-8-sulfamoylisoquinolin-6-yl)methyl)isonicotm^ 6-((4-(((l -aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)-3-methylisoquinoline-8-ca acid;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoqu^^ yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin-6- yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolm^ yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6-yl)methyl)isom N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methyl-8-sulfamoylquinolin-6-yl)methyl)isonicotina 6-((4-((( 1 -aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)-2-methylquinoline-8-carboxylic acid;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolm^ yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6-yl)methyl)isonico N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H^ yl)methyl)benzyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((5-(methylsulfonyl)-6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)isonicotinamide;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((5-(methylsulfonyl)-6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((5-(methylsulfonyl)-6-((2-oxopyridin-l(2H)-yl)methyl)pyri yl)methyl)isonicotinamide;
N-((6-amino-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin^ l(2H)-yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-methyl-2-(trifluoromethyl)pyridin- l(2H)-yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-methyl-2-(trifluoromethyl)pyridin-3 -yl)methyl)-2-(4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotin^ N-((6-amino-2-methyl-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotm N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotm N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methy^
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotina^ N-((6 -amino -4 -methyl -2 -(trifluoromethyl)pyridin-3 -yl)methyl) -2 -((3 -methylquinolin-6 - yl)methyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-fluoroquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide; N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide; N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-fluoroquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide; N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamid N-((6-amino-4-methyl-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-fluoroquinolin-6- yl)methyl)isonicotinamide;
and salts thereof.
Processes for preparing compounds of Formula I and II are provided as further embodiments of the invention and are illustrated by the following Schemes A-D. For example, compounds of Formula I wherein F is -C(=0)NRa- can be prepared as illustrated in Scheme A.
Scheme A
A B C D E COOR
Figure imgf000038_0001
Br E COOR A~B~C~D~E~C( 0)N(Ra)~G~J
A 1 pormuia i
Br~E~C( 0)N(Ra)~G~J
(F is "C( 0)NRa")
A 3
In Scheme A, compounds of Formula I are synthesized by two methods. In one method carboxylic ester A-1 is converted to A-2 through a coupling reaction. Examples of coupling reactions include, but are not limited to, Suzuki reaction, Buchwald reaction, Ullmann reaction, SN(AR), and the like. The ester group in A- 2 is then hydro lyzed and coupled with amine to give target compound of Formula I. In another method, carboxylic acid A-1 is first coupled with a requsite amine to give A-3, which is converted to the target compound of Formula I.
Compounds of Formula I wherein F is
Figure imgf000038_0002
can be prepared as shown in Scheme B.
Scheme B
Br"E"S(0)2 "Cl ► Br"E"S(0)2 "N(Ra)"G"J ► A B C D E S( 0)2N(Ra) G J
Formula τ
B 2 (F is S(0)2NRa )
Sulfonyl chloride B-l is reacted with a requsite amine to give B-2. Various coupling reactions can be run as depicted in Scheme A to give the target compound of Formula I.
Compounds of Formula II where F is -C(=0)NRa- can be prepared as illustrated in Scheme C.
Scheme C
Br'E'COOR ► Br~E~C(=0)N(Ra)~G~J ► C"D"E"C(=0)N(Ra)"G"j
C"l c-2 Formula π
(F is "C(=0)NRa")
Carboxylic acid C-l is coupled with a requsite amine to provide C-2. Examples of coupling reactions include, but are not limited to, Suzuki reaction, Buchwald reaction, Ullmann reaction, SN(AR), and the like. Compound C-2 is then converted to the target compound of Formula II.
Compounds of Formula II wherein F is
Figure imgf000038_0003
can be prepared as shwon in Scheme D. Scheme D
step l step 2 _
Br"E"S(0)2 "Cl ► Br"E"S(0)2 "N(Ra)"G"j ► C"D"E"S("0)2N(Ra)"G"j
- Formula π
D"2 (F is "S(0)2NRa")
Sulfonyl chloride D-l is reacted with a requsite amine to give D-2. Various coupling reactions can be run as depicted in Scheme A to give the target compound of Formula II.
In cases where compounds are sufficiently basic or acidic, a salt of a compound of Formula I or II can be useful as an intermediate for isolating or purifying a compound of Formula I or II. Additionally, administration of a compound of Formula I or II as a pharmaceutically acceptable acid or base salt may be appropriate. Examples of pharmaceutically acceptable salts are organic acid addition salts formed with acids which form a physiological acceptable anion, for example, tosylate, methanesulfonate, acetate,
trifluoroacetate, citrate, malonate, tartrate, succinate, benzoate, behendate, ascorbate, a-ketoglutarate, and a- glycerophosphate. Suitable inorganic salts may also be formed, including hydrochloride, sulfate, nitrate, bicarbonate, and carbonate salts. The compounds may also be isolated as solvates.
Pharmaceutically acceptable salts may be obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound such as an amine with a suitable acid affording a physiologically acceptable anion. Alkali metal (for example, sodium, potassium or lithium) or alkaline earth metal (for example calcium) salts of carboxylic acids can also be made.
The compounds of Formula I or II can be formulated as pharmaceutical compositions and
administered to a mammalian host, such as a human patient in a variety of forms adapted to the chosen route of administration, i.e., orally or parenterally, by intravenous, intramuscular, topical or subcutaneous routes.
Thus, the present compounds may be systemically administered, e.g., orally, in combination with a pharmaceutically acceptable vehicle such as an inert diluent or an assimilable edible carrier. They may be enclosed in hard or soft shell gelatin capsules, may be compressed into tablets, or may be incorporated directly with the food of the patient's diet. For oral therapeutic administration, the active compound may be combined with one or more excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like. Such compositions and preparations should contain at least 0.1% of active compound. The percentage of the compositions and preparations may, of course, be varied and may conveniently be between about 2 to about 60%> of the weight of a given unit dosage form. The amount of active compound in such therapeutically useful compositions is such that an effective dosage level will be obtained. The tablets, troches, pills, capsules, and the like may also contain the following: binders such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, fructose, lactose or aspartame or a flavoring agent such as peppermint, oil of wintergreen, or cherry flavoring may be added. When the unit dosage form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier, such as a vegetable oil or a polyethylene glycol.
Various other materials may be present as coatings or to otherwise modify the physical form of the solid unit dosage form. For instance, tablets, pills, or capsules may be coated with gelatin, wax, shellac or sugar and the like. A syrup or elixir may contain the active compound, sucrose or fructose as a sweetening agent, methyl and propylparabens as preservatives, a dye and flavoring such as cherry or orange flavor. Of course, any material used in preparing any unit dosage form should be pharmaceutically acceptable and substantially non- toxic in the amounts employed. In addition, the active compound may be incorporated into sustained-release preparations and devices.
The active compound may also be administered intravenously or intraperitoneally by infusion or injection. Solutions of the active compound or its salts can be prepared in water, optionally mixed with a nontoxic surfactant. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, triacetin, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
The pharmaceutical dosage forms suitable for injection or infusion can include sterile aqueous solutions or dispersions or sterile powders comprising the active ingredient which are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or dispersions, optionally encapsulated in liposomes. In all cases, the ultimate dosage form should be sterile, fluid and stable under the conditions of manufacture and storage. The liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising, for example, water, ethanol, a polyol (for example, glycerol, propylene glycol, liquid
polyethylene glycols, and the like), vegetable oils, nontoxic glyceryl esters, and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the formation of liposomes, by the maintenance of the required particle size in the case of dispersions or by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, buffers or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and/or gelatin.
Sterile injectable solutions are prepared by incorporating the active compound in the required amount in the appropriate solvent with various other ingredients enumerated above, as desired, followed by filter sterilization. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and the freeze drying techniques, which yield a powder of the active ingredient plus any additional desired ingredient present in the previously sterile-filtered solutions.
For topical administration, the present compounds may be applied in pure form, i.e., when they are liquids. However, it will generally be desirable to administer them to the skin as compositions or
formulations, in combination with a dermatologically acceptable carrier, which may be a solid or a liquid.
Useful solid carriers include finely divided solids such as talc, clay, microcrystalline cellulose, silica, alumina and the like. Useful liquid carriers include water, alcohols or glycols or water-alcohol/glycol blends, in which the present compounds can be dissolved or dispersed at effective levels, optionally with the aid of non-toxic surfactants. Adjuvants such as fragrances and additional antimicrobial agents can be added to optimize the properties for a given use. The resultant liquid compositions can be applied from absorbent pads, used to impregnate bandages and other dressings, or sprayed onto the affected area using pump-type or aerosol sprayers.
Thickeners such as synthetic polymers, fatty acids, fatty acid salts and esters, fatty alcohols, modified celluloses or modified mineral materials can also be employed with liquid carriers to form spreadable pastes, gels, ointments, soaps, and the like, for application directly to the skin of the user.
Examples of dermato logical compositions for delivering active agents to the skin are known to the art; for example, see U.S. Patent Nos. 4,992,478 (Geria), 4,820,508 (Wortzman), 4,608,392 (Jacquet et al.), and 4,559,157 (Smith et al.). Such dermatological compositions can be used in combinations with the compounds described herein where an ingredient of such compositions can optionally be replaced by a compound described herein, or a compound described herein can be added to the composition.
Useful dosages of the compounds of Formula I or II can be determined by comparing their in vitro activity, and in vivo activity in animal models. Methods for the extrapolation of effective dosages in mice, and other animals, to humans are known to the art; for example, see U.S. Pat. No. 4,938,949 (Borch et al.). The amount of the compound, or an active salt or derivative thereof, required for use in treatment will vary not only with the particular salt selected but also with the route of administration, the nature of the condition being treated and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or clinician.
In general, however, a suitable dose will be in the range of from about 0.5 to about 100 mg/kg, e.g., from about 10 to about 75 mg/kg of body weight per day, such as 3 to about 50 mg per kilogram body weight of the recipient per day, preferably in the range of 6 to 90 mg/kg/day, most preferably in the range of 15 to 60 mg/kg/day.
The compound is conveniently formulated in unit dosage form; for example, containing 5 to 1000 mg, conveniently 10 to 750 mg, most conveniently, 50 to 500 mg of active ingredient per unit dosage form. In one embodiment, the invention provides a composition comprising a compound of the invention formulated in such a unit dosage form.
The desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example, as two, three, four or more sub-doses per day. The sub-dose itself may be further divided, e.g., into a number of discrete loosely spaced administrations; such as multiple inhalations from an insufflator or by application of a plurality of drops into the eye.
A disease or condition in an animal wherein inhibition of plasma kallikrein is indicated includes the diseases and conditions recited above in the Background section above. A specific condition where inhibition of plasma kallikrein is indicated is genetic angioedema or 'hereditary angioedema' ("HAE").
The ability of a compound of the invention to inhibit plasma kallikrein may be determined using pharmacological models which are well known to the art, or using Test A described below.
Test A.
A 10 mM solution of a test compound ('Compound X') was made in DMSO. This solution was serially diluted 1 in 5 in DMSO to yield 2000, 400, 80, 16, 3.2, 0.64, 0.128, 0.0256 and 0.00512 μΜ solutions, hereby referred to as "100X Compound X DMSO stocks". A control tube containing only DMSO was included. 16 μΐ. of each 100X DMSO stock dilution was combined with 384 μΐ. of assay buffer (50 mM Tris- HC1 pH 7.5, 150 mM NaCl, 0.01% Triton X-100) to yield a "4X Compound X buffer stock".
Separately, a 40 nM solution of human Plasma Kallikrein (Abeam) and a 93.6 μΜ solution Pro-Phe- Arg-AMC (Bachem) were made using assay buffer. These solutions are hereby known as 4X hPK and 2X PFR-AMC, respectively.
60 μΐ. of each 4X Compound X Buffer stock was combined with 60μΙ. of 4X hPK to yield 120 μΐ. of "2X Compound X Buffer stock/2X hPK". 50 μΐ. was removed from this mixture and placed into duplicate wells on a Microfluor 1 Black U-bottom microtiter plate (Thermo Scientific). This plate was incubated for 5 minutes at 37 °C. To each well, 50 μΐ. of pre-warmed 2X PFR-AMC was added to start the enzymatic reaction. Cleavage of PFR-AMC was monitored in a Bio tek Synergy H4 reader set at 37 °C. Readings were taken every 43 seconds for 1 hour. The highest mean velocity over 20 reads (~15 minutes) was used to calculate the IC50. The IC50 was calculated using the Gen5 (Biotek Instruments).
The invention will now be illustrated by the following non-limiting Examples.
EXAMPLES
Example 1: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)benzamide
Figure imgf000042_0001
To a stirred solution of boron trichloride in toluene (200 mL, 1 M, 0.2 mol, 1.16 eq), a solution of 4- chloroaniline (22.0 g, 0.172 mol, 1.0 eq) in dry toluene (200 mL) was added dropwise under nitrogen at a temperature ranging from 5 °C to 10 °C. To the resulting mixture, chloroacetonitrile (15 mL, 0.237 mol, 1.38 eq) and aluminum trichloride (29.0 g, 0.217 mol, 1.26 eq) were added successively. The mixture was refluxed for 18 h. After cooling, ice-cold hydrochloric acid (2 N, 500 mL) was added and a yellow precipitate was formed. The mixture was warmed at 80 °C with stirring, until the precipitate was dissolved. The cooled solution was extracted with dichloromethane (250 mL X 3). The organic layer was washed with water, dried (Na2SO i), and concentrated. The resulting residue was purified on silica gel column (PE/EtOAc = 50/1 ~ PE/EA/DCM = 1/8/1, v/v/v) to afford l-(2-amino-5-chlorophenyl)-2-chloroethanone as a yellow brown solid (18.3 g, 52 %).
Figure imgf000043_0001
To a stirred suspension of l-(2-amino-5-chlorophenyl)-2-chloroethanone (16 g, 78 mmol, 1.0 eq) in cone, hydrochloric acid (120 mL) was added a solution of sodium nitrite (5.9 g, 86 mmol, 1.1 eq) in water (30 mL) at 0 °C. After 1 h, a solution of SnCl22H20 (42.3 g, 187 mmol, 2.4 eq) in cone, hydrochloric acid (60 mL) was added to the reaction mixture and stirred for 1 h. Ice-water was added to the reaction mixture. The precipitate was collected by filtration, washed with water and dried to afford crude 5-chloro-3-(chloromethyl)- lH-indazole, which was used in the next step without further purification (13.5 g, 86%).
Figure imgf000043_0002
A solution of 5-chloro-3-(chloromethyl)-lH-indazole (13.5 g, 67 mmol, 1.0 eq), 3,4-dihydro-2H-pyran (11.3 g, 134 mmol, 2.0 eq) and p-toluenesulfonic acid monohydrate (1.27 g, 6.7 mmol, 0.1 eq) in THF (300 mL) was stirred at 70 °C for 12 h. After cooling to rt (~22 °C), the reaction mixture was mixed with water (300 mL) and extracted with EA (200 mL x2). The organic layer was washed with brine, dried over anhydrous Na2S04, and concentrated to afford 5-chloro-3-(chloromethyl)-l -(tetrahydro-2H-pyran-2-yl)-lH-indazole as a yellow solid (16 g, 84%
Figure imgf000043_0003
To a solution of 5-chloro-3-(chloromethyl)-l-(tetrahydro-2H-pyran-2-yl)-lH-indazole (18 g, 63 mmol, 1 eq) in anhydrous DMF (200 mL) under N2, potassium phthalimide (17.5 g, 94 mmol, 1.5 eq) was added and the resulting mixture was heated at 90 °C for 2 h. The mixture was poured into water and extracted with DCM (200 mL X 2). The combined organic layers were washed with water and brine, dried over anhydrous Na2S04 and concentrated. The crude product was washed with EtOH to afford 2-((5-chloro-l -(tetrahydro-2H- pyran-2-yl)-lH-indazol-3-yl)methyl)isoindoline-l,3-dione as a white solid (15 g, 60%>).
Figure imgf000043_0004
To a solution of 2-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)isoindoline-l,3-dione (15 g, 37.9 mmol, 1.0 eq) in THF (300 mL) and DCM (60 mL) was added hydrazine hydrate (9.5 g, 189 mmol, 5 eq). The white suspension was stirred at 48 °C for 12 h and phthalyl hydrazide was removed by filtration. The filtrate was concentrated in vacuo and the crude material was dissolved in DCM and washed with 1 N NaOH solution. The organic layer was dried over anhydrous Na2S04 and concentrated to afford (5-chloro-l- (tetrahydro-2H-pyran-2-yl)-l -indazol-3-yl)methanamine as a ellow solid (9.9 g, 99%).
Figure imgf000044_0001
The mixture of pyridin-2(lH)-one (6.0 g, 63.1 mmol, 1.0 eq), 1 ,4-bis(bromomethyl)benzene (50.0 g, 189.4 mmol, 3.0 eq) and potassium carbonate (8.7 g, 63.1 mmol, 1.0 eq) in C¾CN (350 mL) was stirred at 80 °C overnight. After cooled to rt, to the mixture was added water (300 mL). The precipitate was filtered and rinsed with EtOH to recover 1 ,4-bis(bromomethyl)benzene; the filtrate was extracted with DCM (100 mL X 2), the extracts were washed with water, brine and dried over anhydrous Na2S04, filtered and concentrated to afford l-(4-(br
Figure imgf000044_0002
A mixture of ethyl 3-bromobenzoate (2.29 g, 10 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l ,3,2- dioxaborolane) (3.05 g, 12 mmol, 1.2 eq), Pd(dppf)Cl2 (732 mg, 1 mmol, 0.1 eq), KOAc (2.94 g, 30 mmol, 3 eq) in DME (50 mL) under N2 was stirred at 90 °C overnight and concentrated. The resulting residue was purified via flash chromatography (PE/EA = 50/1, v/v) to afford ethyl 3-(4,4,5,5-tetramethyl-l,3,2- dioxaborolan-2-yl)be
Figure imgf000044_0003
A mixture of ethyl 3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)benzoate (2.64 g, 9.57 mmol, 1.16 eq), 1- (4-(bromomethyl)benzyl)pyridin-2(lH)-one (2.30 g, 8.27 mmol, 1 eq), Pd(PPh3)4 (1.16 g, 1 mmol, 0.12 eq), K2CO3 (4.14 g, 30 mmol, 3.6 eq) in DME/H2O (60 mL/15 mL) under N2 was stirred at 90 °C overnight and concentrated. The resulting residue was extracted with EA (50 mL X 3). The combined organic layers were dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via flash chromatography (PE/EA=9/1-1/1, v/v) to afford ethyl 3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzoate as a colorless oil (3.10 g crude, quant).
Figure imgf000045_0001
To a solution of ethyl 3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzoate (3.10 g, crude, 1.0 eq) in MeOH (15 mL) and THF (10 mL) was added 2 N NaOH aqueous solution (20 mL). The mixture was stirred at rt overnight. The mixture was concentrated. To the resulting residue was added cone. HCl to adjust to pH 2. The precipitate was collected and dried to afford 3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzoic acid as a white solid (2.64 g, quant.).
Figure imgf000045_0002
A mixture of 3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzoic acid (320 mg, 1 mmol, 1 eq), (5-chloro-l - (tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (320 mg crude, 1.2 mmol, 1.2 eq), HATU (570 mg, 1.5 mmol, 1.5 eq) and DIEA (1 mL) in DMF (5 mL) was stirred at 60 °C overnight. The mixture was poured into water (20 mL). The precipitate was collected and purified via flash chromatography to afford N-((5- chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-3 -(4-((2-oxopyridin- 1 (2H)- yl)methyl)b
Figure imgf000045_0003
A solution of N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)-l H-indazol-3 -yl)methyl)-3 -(4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)benzamide (100 mg, 0.176 mmol, 1 eq) in MeOH (3 mL) and HCl/dioxane (5 N, 2 mL) was stirred at rt overnight, then concentrated. The resulting residue was suspended in NaHC03 solution. The precipitate was collected and purified via prep-TLC (DCM/MeOH = 10/1, v/v) to afford N-((5-chloro-lH- indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide as a yellow solid (60 mg, 70%). LRMS (M+H+) m/z calculated 483.2, found 483.2. ¾ NMR (DMSO-i 6, 300 MHz) δ 13.06 (s, 1 H), 9.09 (t, 1 H), 7.90 (d, 1 H), 7.75-7.67 (m, 3 H), 7.52 (d, 1 H), 7.42-7.31 (m, 4 H), 7.20 (s, 4 H), 6.38 (d, 1 H) , 6.21 (t-d, 1 H), 5.03 (s, 2 H), 4.76 (d, 2 H), 3.94 (d, 2 H). Example 2: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)amino)benzamide
Figure imgf000046_0001
N"((5"ch|oro"i H dazo 3"y|)methy|)~3"((4"((2^
Figure imgf000046_0002
To a solution of l-(bromomethyl)-4-nitrobenzene (2.3 g, 10.5 mmol, 1.0 eq) in CH3CN (20 mL) were added K2CO3 (2.9 g, 21 mmol, 2.0 eq) and pyridin-2(lH)-one (1.0 g, 10.5 mmol, 1.0 eq). The mixture was heated at 80 °C overnight. The mixture was concentrated under reduced pressure, mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via silica gel column (PE/EA = 5/1 to DCM/MeOH = 200/1, v/v) to afford l-(4-nitrobenzyl)pyridin-2(lH)-one as a yellow solid (1.9 g, 79 %).
Figure imgf000046_0003
To a solution of l-(4-nitrobenzyl)pyridin-2(lH)-one (1.9 g, 8.3 mmol, 1.0 eq) in EtOH (20 mL) and H20 (20 mL) was added Fe (1.9 g, 33 mmol, 4.0 eq). The mixture was heated to 80 °C, con.HCl (0.1 mL, 0.8 mmol, 0.1 eq) was then added dropwise. The resulting mixture was heated at 80 °C overnight. The mixture was adjusted to pH 10 with 15% KOH in EtOH, and filtered. The filter cake was washed with EtOH (20 mL X 2). The combined filtrate was concentrated to remove EtOH, the aqueous residue was extracted with EA (50 mL X 6). The combined organic layers were washed with brine (30 mL), dried over anhydrous Na2S04 and
concentrated. The resulting residue was purified via silica gel column (DCM/MeOH= 200/1 to 100/1, v/v) to afford l-(4-aminobenzyl)pyridin-2(lH)-one as a yellow solid (1.3 g, 79%).
Figure imgf000047_0001
To a solution of l-(4-aminobenzyl)pyridin-2(lH)-one (500 mg, 2.5 mmol, 1.0 eq.) in toluene (15 mL) were added ethyl 3-bromobenzoate (630 mg, 2.7 mmol, 1.1 eq), K3P04 (1.06 g, 5 mmol, 2.0 eq), Pd2(DBA)3 (36 mg, 0.0375 mmol, 0.015 eq) and X-phos (58 mg, 0.1 mmol, 0.04 eq) at rt. The mixture was heated at 120 °C overnight under N2. After cooled to 25 °C, the mixture was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via silica gel column (DCM/MeOH = 200/1, v/v) to afford ethyl 3-((4-( -oxopyridin-l(2H)-yl)methyl)phenyl)amino)benzoate as a white solid (460 mg, 53%).
Figure imgf000047_0002
To a solution of ethyl 3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)benzoate (460 mg, 1.3 mmol, 1.0 eq) in MeOH (4 mL) and H2O (4 mL) was added NaOH (160 mg, 4 mmol, 3.0 eq). The mixture was heated at 60 °C for 30 min. The mixture was concentrated to remove MeOH, then diluted with ice-water (10 mL) and acidified with 1 N HC1 to pH 2~3. The mixture was concentrated under reduced pressure. The resulting residue was re-dissolved in MeOH (20 mL) and filtered. The filtrate was concentrated to afford 3-((4-((2- oxopyridin-l(2 ).
Figure imgf000047_0003
To a solution of 3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)benzoic acid (200 mg, 0.63 mmol, 1.0 eq) in DMF (5 mL) were added DIPEA (242 mg, 1.88 mmol, 3.0 eq) and HATU (285 mg, 0.75 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methanamine (166 mg, 0.63 mmol, 1.0 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via prep-TLC (DCM/MeOH = 20/1, v/v) to afford N-((5-chloro-l- (tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-3 -((4-((2-oxopyridin- 1 (2H)- yl)methyl)pheny
Figure imgf000048_0001
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin- l(2H)-yl)methyl)phenyl)amino)benzamide (100 mg, 0.18 mmol, 1.0 eq) in MeOH (2 mL) was added HC1/EA (2 mL, 4 mmol) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated under reduced pressure. The resulting residue was mixed with water (10 mL), neutralized with saturated NaHCCb (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2SC>4 and concentrated. The resulting residue was purified via prep-TLC (DCM/MeOH = 20/1, v/v) to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)amino)benzamide as a white solid (40 mg, 47%). LRMS (M+H+) m/z caculated 484.2, found 484.2. ¾ NMR (DMSO-ifc, 300 MHz) δ 13.04 (brs, 1 H), 9.00-9.05 (m, 1 H), 8.36 (s, 1 H), 7.90 (d, 1 H), 7.70-7.80 (m, 1 H), 7.50-7.60 (m, 2 H), 7.10-7.45 (m, 7 H), 7.00-7.05 (m, 2 H), 6.39 (d, 1 H), 6.22 (t, 1 H), 4.99 (s, 2 H), 4.76 (d, 2 H).
Example 3: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-((4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)amino)nicotinamide
Figure imgf000048_0002
N ((5 chioro 1 H jndazoi 3 yi nethyl) 5 ((4 ((2 oxopyrjdin 1 (2H) yljmethyljphenyijamjnojnjcotinamjde
Figure imgf000048_0003
To a solution of l-(4-aminobenzyl)pyridin-2(lH)-one (500 mg, 2.5 mmol, 1.0 eq) in toluene (15 mL) were added ethyl 5-bromonicotinate (633 mg, 2.7 mmol, 1.1 eq), K3PO4 (1.06 g, 5 mmol, 2.0 eq), Pd2(DBA)3 (36 mg, 0.0375 mmol, 0.015 eq) and X-phos (58 mg, 0.1 mmol, 0.04 eq) at rt. The mixture was heated at 120 °C overnight under N2. After cooled to 25 °C, the mixture was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via silica gel column (DCM/MeOH = 200/1, v/v) to afford ethyl 5-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)nicotinate as a yellow solid (350 mg, 40%).
Figure imgf000049_0001
To a solution of ethyl 5-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)nicotinate (350 mg, 1 mmol, 1.0 eq) in MeOH (5 mL) and H2O (5 mL) was added NaOH (120 mg, 3 mmol, 3.0 eq). The mixture was heated at 60 °C for 30 min. The mixture was concentrated to remove MeOH, then diluted with ice-water (10 mL) and acidified with 1 N HC1 to pH 2~3. The mixture was concentrated under reduced pressure. The resulting residue was re-dissolved in MeOH (20 mL) and filtered. The filtrate was concentrated to afford 5-((4-((2- oxopyridin-l(2 ant.).
Figure imgf000049_0002
To a solution of 5-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)nicotinic acid (400 mg crude, 1 mmol, 1.0 eq) in DMF (5 mL) were added DIPEA (387 mg, 3 mmol, 3.0 eq) and HATU (456 mg, 1.2 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methanamine (266 mg, 1 mmol, 1.0 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via flash chromatography to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2 yl)-lH-indazol-3-yl)methyl)-5-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)nicotinamide as a white solid (300 mg, 53%).
Figure imgf000050_0001
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-((4-((2-oxopyridin- l(2H)-yl)methyl)phenyl)amino)nicotinamide (300 mg, 0.53 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/EA (2 mL, 4 mmol) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated under reduced pressure. The resulting residue was mixed with water (10 mL), neutralized with saturated NaHC03 (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-((4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)amino)nicotinamide as a white solid (30 mg, 12%). LRMS (M+H+) m/z caculated 485.2, found 485.2. ¾ NMR (DMSO-ifc, 300 MHz) δ 9.20-9.27 (m, 1 H), 8.58 (s, 1 H), 8.45 (s, 1 H), 8.39 (d, 1 H), 7.90 (s, 1 H), 7.80-7.84 (m, 1 H), 7.78 (d, 1 H), 7.53 (d, 1 H), 7.40 (t, 1 H), 7.33 (d, 1 H), 7.25 (d, 2 H), 7.08 (d, 2 H), 6.40 (d 1 H), 6.22 (t, 1 H), 5.01 (s, 2 H), 4.77 (d, 2 H).
Example 4: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l
yl)methyl)b
Figure imgf000050_0002
The mixture of (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (2.8 g, 10.7 mmol, 1.0 eq), 3-bromobenzene-l-sulfonyl chloride (3.0 g, 11.7 mmol, 1.1 eq) and triethylamine (3.24 g, 32.1 mmol, 3.0 eq) in DCM (50 mL) was stirred at rt overnight. Then the mixture was washed with water and brine, dried over anhydrous sodium sulfate, filtered and concentrated. The resulting residue was purified on silica gel column (PE/EA = 2: 1, v/v) to afford 3-bromo-N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)benzenesulfonamide as a yellow oil (4.8 g, 84%).
Figure imgf000051_0001
The minture of 3-bromo-N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)benzenesulfonamide (500 mg, 1.03 mmol, 1.1 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l ,3,2- dioxaborolane) (314 mg, 1.24 mmol, 1.2 eq), Pd(dppf)Cl2 (73 mg, 0.1 mmol, 0.1 eq) and KOAc (304 mg, 3.1 mmol, 3.0 eq) in 1 ,2-dimethoxy ethane (20 mL) was stirred at 90 °C under N2 overnight. Then the mixture was concentrated. The resulting residue was diluted with EtOAc (50 mL), washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated. The resulting residue was purified on silica gel column (PE/EtOAc = 2/1, v/v) to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3- (4,4,5,5-
Figure imgf000051_0002
The minture of N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-3 -(4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl)benzenesulfonamide (800 mg, 1.5 mmol, 1.2 eq), l-(4-
(bromomethyl)benzyl)pyridin-2(lH)-one (350 mg, 1.26 mmol, 1.0 eq), Pd(dppf)Cl2 (95 mg, 0.13 mmol, 0.1 eq) and K3PO4 (801 mg, 3.78 mmol, 3.0 eq) in DMF/H2O (20 mL/2 mL) was stirred at 110 °C under N2 overnight. Then the mixture was concentrated. The resulting residue was diluted with EtOAc (50 mL), washed with brine, dried over anhydrous sodium sulfate, filtered and concentrated. The resulting residue was purified by silica gel column (PE/EtOAc = 1/1, v/v) to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol- 3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzenesulfonamide as a yellow solid (360 mg, 47%).
Figure imgf000051_0003
N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-3-(4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)benzenesulfonamide (360 mg, 0.6 mmol, 1.0 eq) was dissolved in HCl/l,4-dioxane (2 M, 20 mL) and stirred at rt for 2 h. Then the mixture was concentrated in vacuo, and the resulting residue was purified by pre-HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)benzenesulfonamide as yellow solid (27.8 mg, 9%). LCMS (M+H+) m/z calculated 519.1, found 518.8. ¾ NMR (CD3OD, 300 MHz) δ 7.68-7.57 (m, 3H), 7.54-7.48 (m, 1H), 7.39 (d, 1H), 7.34-7.22 (m, 5H), 7.14 (d, 1H), 6.57 (d, 1H), 6.37 (dt, 1H), 5.16 (s, 2H), 4.40 (s, 2H), 3.91 (d, 1H).
Example 5: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)pyridine-3-sulfonamide
Figure imgf000052_0001
chioro 1 H indazoi 3 yKmethyh 5 (4 ((2 oxopyridin - 2H) yhmethyhbenzyKpynd ine 3
sulfonamide
Figure imgf000052_0002
Potassium acetate (1.9 g, 19.4 mmol, 3.0 eq), bis(pinacolato)diboron ( 1.98 g, 7.76 mmol, 1.2 eq) and tetrakis(triphenylphosphine)palladium (374 mg, 0.323 mmol, 0.05 eq) were added to a solution of l -(4-
(bromomethyl)benzyl)pyridin-2(lH)-one (1.8 g, 6.47 mmol, 1.0 eq) in dioxane (120 mL) and the mixture was heated at 80 °C for 3 h and allowed to cool. The mixture was concentrated, and the resulting residue was purified on silica gel column (PE/EA = 3/1 then DCM/MeOH = 15/1, v/v) to afford l-(4-((4,4,5,5- tetramethyl-l,3,2-dioxaborolan-2-yl)methyl)benzyl)pyridin-2(lH)-one as a white solid (1.0 g, 47%).
Figure imgf000052_0003
A mixture of 5-bromopyridine-3-sulfonyl chloride (257 mg, 1 mmol, 1.0 eq), (5-chloro-l-(tetrahydro-2H- pyran-2-yl)-lH-indazol-3-yl)methanamine (310 mg, 12 mmol, 1.2 eq) and Et3N (l mL) in DCM (10 mL) was stirred at rt overnight and then concentrated. The resulting residue was purified via flash chromatography (PE/EA/DCM=l/2/l, v/v/v) to afford 5-bromo-N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)pyridine-3-sulfonamide as a white solid (451 mg, 92%).
Figure imgf000053_0001
A mixture of 5-bromo-N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)pyridine-3- sulfonamide (99 mg, 0.20 mmol, 1.0 eq), l -(4-((4,4,5,5-tetramethyl-l ,3,2-dioxaborolan-2- yl)methyl)benzyl)pyridin-2(lH)-one (85 mg, 0.26 mmol, 1.3 eq), Pd(PPh3)4 (27 mg, 0.023 mmol, 0.1 15 eq), Na2CC>3 (106 mg, 1 mmol, 5 eq) in 1 ,4-dioxane/H20 (5 mL/0.5 mL) under N2 was stirred at 90 °C overnight and then concentrated. The resulting residue was purified via prep-TLC (DCM/MeOH = 12/1 , v/v) to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)pyridine-3-sulfonamide as a white solid (92 mg, 74%).
Figure imgf000053_0002
A solution of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)pyridine-3-sulfonamide (92 mg, 0.15 mmol, 1 eq) in MeOH (2 mL) and HCl/dioxane (5 N, 2 mL) was stirred at rt overnight, then concentrated. The resulting residue was suspended in NaHCC solution. The precipitate was collected and purified via prep-TLC (DCM/MeOH = 12/1 , v/v) to afford N-((5- chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)pyridine-3-sulfonamide as a white powder (50 mg, 63%). LRMS (M+H+) m/z calculated 520.1 , found 520.1. ¾ NMR (CD30D-i/6, 300 MHz) δ 8.56 (d, 1 H), 8.34 (d, 1 H), 7.70-7.65 (m, 3 H), 7.56-7.50 (m, 1 H), 7.38 (d, 1 H), 7.31 -7.25 (m, 3 H), 7.14 (s, 1 H), 7.12 (s, 1 H), 6.57 (d, 1 H) , 6.39 (t, 1 H), 5.18 (s, 2 H), 4.51 (s, 2 H), 3.88 (s, 2 H).
Example 6: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-((4-((2-oxopyridin-l (2H)- yl)methyl)phenyl)amino)pyridine-3 -sulfonamide
Figure imgf000053_0003
N ((5 chioro -i n indazoi 3 yhmethyk 5 ((4 ((2 oxopyHdin -W2H) yhmethyKphenyhaminojpyndine 3
sulfonamide
Figure imgf000054_0001
A mixture of 5-bromo-N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)pyridine-3- sulfonamide (243 mg, 0.5 mmol, 1.0 eq), l-(4-aminobenzyl)pyridin-2(lH)-one (121 mg, 0.6 mmol, 1.2 eq), Pd2(dba)3 (50 mg, 0.05 mmol, 0.1 eq), K3PO4 (318 mg, 1.5 mmol, 3 eq) in toluene (5 mL) under N2 was refluxed overnight and then filtered. The filtrate was concentrated. The resulting residue was purified via prep-TLC (DCM/MeOH = 12/1, v/v) to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)-5-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)pyridine-3-sulfonamide as a light yellow solid (72 mg
Figure imgf000054_0002
A solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-((4-((2-oxopyridin- l(2H)-yl)methyl)phenyl)amino)pyridine-3 -sulfonamide (72 mg, 0.12 mmol, 1 eq) in MeOH (2 mL) and HCl/dioxane (5 N, 1 mL) was stirred at rt overnight, then concentrated. The resulting residue was triturated with Et20 (5 mL) and DCM (10 mL) to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-((4-((2-oxopyridin- l(2H)-yl)methyl)phenyl)amino)pyridine-3 -sulfonamide HC1 salt as a yellow powder (60 mg, 90%). LRMS (M+H+) m/z calculated 521.1, found 520.8. ¾ NMR (CD30D-i/6, 300 MHz) δ 8.13 (d, 2 H), 7.93 (dd, 1 H), 7.84-7.83 (m, 1 H), 7.74-7.69 (m, 2 H), 7.45-7.41 (m, 3 H), 7.33-7.30 (m, 1 H), 7.17-7.14 (m, 2 H), 6.75 (d, 1 H) , 6.64 (t-d, 1 H), 5.30 (s, 2 H), 4.64 (s, 2 H).
Example 7: Preparation ofN-((5-chloro-lH-indazol-3-yl)methyl)-l -(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-lH-pyrrolo[2,3-b]pyri
Figure imgf000054_0003
W"((5"ch|oro"iH dazof3"ykmethyhT^
carboxamjde
Figure imgf000055_0001
To a solution of lH-pyrrolo[2,3-b]pyridine-3-carboxylic acid (162 mg, 1 mmol, 1.0 eq) in DMF (8 mL) were added HATU (570 mg, 1.5 mmol, 1.5 eq) and Et3N (0.3 mL, 2.0 mmol, 2.0 eq). The mixture was stirred at rt for 15 min, (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (265 mg, 1.0 mmol, 1.0 eq) was then added. The resulting mixture was stirred at rt overnight, then mixed with water and extracted with DCM (50 mL X 2). The combined organic layers were washed with water and brine, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified via silica gel column (DCM/MeOH = 10/1, v/v) to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-lH-pyrrolo[2,3- b]pyridine-3-
Figure imgf000055_0002
A mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-lH-pyrrolo[2,3-b]pyridine- 3-carboxamide (380 mg, 0.93 mmol, 1.0 eq), l-(4-(bromomethyl)benzyl)pyridin-2(lH)-one (258 mg, 0.93 mmol, 1.0 eq) and Cs2C03 (1.05 g, 1.8 mmol, 2.0 eq) in CH3CN (20 mL) was stirred at 80 °C overnight. The reaction mixture was poured into water and extracted with DCM (50 mL X 2). The combined organic layers were washed with water and brine, dried over anhydrous sodium sulfate and concentrated. The resulting residue was purified via flash chromatography (reverse, 30-95%) Cf¾CN in water) to afford N-((5-chloro-l- (tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-l -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)-l H- pyrrolo[2,3-b]pyri
Figure imgf000055_0003
To a solution of N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)- 1 -(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)-lH-pyrrolo[2,3-b]pyridine-3-carboxamide (130 mg, 0.21 mmol, 1.0 eq) in MeOH (2 mL) was added concentrated HC1 (0.5 mL, 6 mmol). The mixture was stirred at rt for 2 h, and then concentrated in vacuo. The resulting residue was neutralized with saturated aqueous sodium bicarbonate solution, the precipitate was collected by filtration, further purified by preparative TLC (5% MeOH in DCM) to afford N-((5-chloro- 1 H-indazol-3 -yl)methyl)- 1 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H-pyrrolo [2,3 - b]pyridine-3-carboxamide as a white solid. LRMS (M+H+) m/z calculated 523.0, found 522.8. 'H NMR (DMSO-i/6, 300 MHz) δ 13.03 (s, 1 H), 8.69-8.65 (t, 1 H), 8.52-8.50 (d, 1 H), 8.33-8.31 (d, 1 H), 8.24 (s, 1H), 7.92 (s, 1 H), 7.73-7.70 (d, 1H), 7.537.50 (d, 1H), 7.31-7.21 (m, 7H), 6.38-6.35 (d, 1H), 6.20-6.18 (t, 1H), 5.46 (s, 2H), 5.02 (s, 2H), 4.77-4.75 (d, 2H).
Example 8: Preparation ofN-((5-chloro-lH-indazol-3-yl)methyl)-l -(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-lH-indazole-3-carboxamide
Figure imgf000056_0001
W"((5"ch|oro"i H dazof3"y|)methyl)T(4"((2^^
Figure imgf000056_0002
To a solution of lH-indazole-3-carboxylic acid (324 mg, 2.0 mmol, 1.0 eq) in DMF (10 mL) were added HATU (1.14 g, 3.0 mmol, 1.5 eq) and DIEA (768 mg, 6.0 mmol, 3.0 eq). The mixture was stirred at rt for 30 min. Then (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (584 mg, 2.2 mmol, 1.1 eq) was added. The resulting mixture was stirred at 80 °C overnight. The mixture was poured into water and extracted with EtOAc (50 mL X 2). The combined organic layers were washed with water and brine, dried over anhydrous sodium sulfate, filtered and concentrated. The resulting residue was purified on silica gel column (PE/EtOAc = 5/1, v/v) to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)- lH-indazole-3-carboxamide as a yellow solid (410 mg, 50%).
Figure imgf000057_0001
A mixture of N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)-l H-indazol-3-yl)methyl)- 1 H-indazole-3 - carboxamide (410 mg, 1.0 mmol, 1.0 eq), l-(4-(bromomethyl)benzyl)pyridin-2(lH)-one (278 mg, 1.0 mmol, 1.0 eq) and K2C03 (276 mg, 2.0 mmol, 2.0 eq) in DMF (10 mL) was stirred at 90 °C overnight. After cooled to rt, the mixture was poured into water. The precipitate was collected and dried to afford N-((5-chloro-l - (tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-l -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H- indazole-3 -carbox
Figure imgf000057_0002
A solution of N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)- 1 -(4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)-l H-indazole-3 -carboxamide (405 mg, 0.67 mmol, 1.0 eq) in HCl/l,4-dioxane (~2 M, 10 mL) was stirred at rt overnight. The mixture was concentrated and the resulting residue was purified by preparative HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-l-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-l H-indazole-3 -carboxamide as yellow solid. LCMS (M+H+) m/z calculated 523.1, found 522.8. 'H NMR (CD3OD, 300 MHz) δ 8.26 (d, 1H), 7.96 (s, 1H), 7.61 (d, 1H), 7.56-7.46 (m, 3H), 7.40 (t, 1H), 7.35-7.25 (m, 2H), 7.22 (s, 4H), 6.54 (d, 1H), 6.35 (t, 1H), 5.67 (s, 2H), 5.13 (s, 2H), 4.96 (s, 2H).
Example 9: Preparation ofN-((5-chloro-lH-indazol-3-yl)methyl)-l -(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)piperidine-3-carboxamide
Figure imgf000057_0003
N ((5 chioro 1 H indazoi 3 yl^methyl) 1 (4 ((2 oxopyrjdin - {2H) yl)methyl)benzy|)pjperjdine 3 car oxamide
Figure imgf000058_0001
To a solution of l-(4-(bromomethyl)benzyl)pyridin-2(lH)-one (100 mg, 0.36 mmol, 1.0 eq) in CftCN (5 mL) were added K2CO3 (149 mg, 1.08 mmol, 3.0 eq) and ethyl piperidine-3-carboxylate (113 mg, 0.72 mmol, 2.0 eq). The mixture was heated at 80 °C overnight, and then concentrated. The resulting residue was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via flash
chromatography to afford ethyl l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3-carboxylate as a colorless oil (100 mg,
Figure imgf000058_0002
To a solution of ethyl l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3-carboxylate (100 mg, 0.28 mmol, 1.0 eq) in MeOH (2 mL) and H20 (2 mL) was added NaOH (34 mg, 0.85 mmol, 3.0 eq). The mixture was heated at 60 °C for 30 min. The mixture was concentrated to remove MeOH, then diluted with ice-water (10 mL) and acidified with 1 N HC1 to pH 2~3. The mixture was concentrated under reduced pressure. The resulting residue was re-dissolved in MeOH (20 mL) and filtered. The filtrate was concentrated under reduced pressure to afford l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3-carboxylic acid as a white solid (200 mg crude, quant.).
Figure imgf000058_0003
To a solution of l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3-carboxylic acid (200 mg crude, 0.28 mmol, 1.0 eq) in DMF (5 mL) were added DIPEA (108 mg, 0.84 mmol, 3.0 eq) and HATU (128 mg, 0.34 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l -(tetrahydro-2H- pyran-2-yl)-lH-indazol-3-yl)methanamine (82 mg, 0.31 mmol, 1.1 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via flash chromatography to afford N-((5-chloro-l- (tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)mem^
3-carboxamide
Figure imgf000059_0001
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-l-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)piperidine-3-carboxamide (70 mg, 0.12 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/EA (2 mL, 4 mmol) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated under reduced pressure. The resulting residue was mixed with water (10 mL), neutralized with saturated NaHC03 (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2S04 and concentrated. The resulting residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3-yl)methyl)-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3- carboxamide as an off-white solid (30 mg, 51%). LRMS (M+H+) m/z caculated 490.2, found 489.9. ¾ NMR (DMSO-ifc, 300 MHz) δ 13.07 (brs, 1 H), 8.40-8.50 (m, 1 H), 7.70-7.80 (m, 2 H), 7.51 (d, 1 H), 7.38-7.45 (m, 1 H), 7.30-7.35 (m, 1 H), 7.10-7.28 (m, 4 H), 6.41 (d, 1 H), 6.20-6.30 (m, 1 H), 5.05 (s, 2 H), 4.52 (d, 2 H), 3.40-3.45 (m, 2 H), 2.60-2.72 (m, 2 H), 2.30-2.40 (m, 1 H), 2.00-2.10 (m, 1 H), 1.80-1.95 (m, 1 H), 1.50-1.70 (m, 2 H), 1.30-1.48 (m, 2 H).
Example 10: Preparation ofN-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000059_0002
N ((5 chioro 1 H indazoi 3 l^methyl) 5 (4 ((2 oxopyHdin 1 (2H) y|)methyl)benzy|)njcotinamjc|e
Figure imgf000059_0003
A mixture of l-(4-((4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)methyl)benzyl)pyridin-2(lH)-one (200 mg, 0.615 mmol, 1.0 eq), ethyl 5-bromonicotinate (170 mg, 0.738 mmol, 1.2 eq), Pd(PPh3) i (71 mg, 0.061 mmol, 0.1 eq), Na2C03 (196 mg, 1.84 mmol, 3.0 eq) in dioxane/H20 (20 mL/2 mL) under N2 was stirred at 95 °C overnight and concentrated. The residue was extracted with EA (50 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via flash
chromatography (MeCN/H20 = 30%-95%, v/v) to afford ethyl 5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinate
Figure imgf000060_0001
To a solution of ethyl 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate (71 mg, 0.204 mmol, 1.0 eq) in MeOH (5 mL) was added drop wise a solution of NaOH (82 mg, 2.04 mmol, 10 eq) in water (3 mL). The mixture was stirred at rt overnight. The organic solvents were evaporated. To the residue was added cone. HCl till pH 5, then extracted with EA (20 mL *3). The combined organic layers were washed with brine, dried over sodium sulfate, filtered and concentrated in vacuo to afford 5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)ni
Figure imgf000060_0002
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (63 mg, 0.196 mmol, 1 eq), (5- chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (63 mg, 0.236 mmol, 1.2 eq), HATU (82 mg, 0.216 mmol, 1.1 eq) and DIEA (51 mg, 0.393 mmol, 2 eq) in DMF (1 mL) was stirred at rt overnight. The mixture was poured into water (20 mL) and saturated NaHC03 (20 mL), extracted with EA (30 mL X 2). The combined organic layers was washed with brine, dried over sodium sulfate, filtered and concentrated in vacuo. The residue was purified by flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a yellow oil (72 mg, 64%>).
Figure imgf000061_0001
A solution of N-((5-chloro -(tetrahydro-2H-pyran-2-yl) H-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)nicotinamide (70 mg, 0.123 mmol, 1 eq) in MeOH (5 mL) and HCl/Et20 (3 N, 5 mL) was stirred at rt overnight, then concentrated. The residue was collected and dried to afford the HCl salt of N-((5- chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (58 mg, 90%). LRMS (M+H+) m/z calculated 484.2, found 484.2. ¾ NMR (DMSO-i/6, 400 MHz) δ 13.09 (s, 1 H), 9.28 (t, 1 H), 8.85 (d, 1 H), 8.61 (d, 1 H), 8.04 (s, 1 H), 7.89 (d, 1 H), 7.73 (q, 1 H), 7.53 (d, 1 H) , 7.41- 7.31 (m, 2 H), 7.24-7.19 (m, 4 H), 6.39 (d, 1 H), 6.20 (t, 1 H), 5.03 (s, 2 H), 4.77 (d, 2 H), 3.98 (s, 2 H). Example 11: Preparation ofN-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)pyrazine-2-carboxami
Figure imgf000061_0002
N ((5 chioro 1 H indazoi 3 l^methyl) 6 (4 ((2 oxopyrjdin <| (2H) yl)methyl)benzy|)Pyrazjne 2 carboxamjde
Figure imgf000061_0003
A mixture of l-(4-((4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)methyl)benzyl)pyridin-2(lH)-one (339 mg, 1.05 mmol, 3.0 eq), methyl 6-chloropyrazine-2-carboxylate (150 mg, 0.87 mmol, 1.0 eq), Pd(dppf)Ci2 (71 mg, 0.061 mmol, 0.1 eq), K2C03 (240 mg, 1.74 mmol, 2.0 eq) in toluene/dioxane/H20 (10 niL/1 niL/1 mL) under N2 was stirred at 100 °C for 5 h and then concentrated. The residue was extracted with EA (50 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via pre-TLC to afford methyl 6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrazine-2-carboxylate as a white solid (100 mg, 34%). N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrazine-2- carboxamide (HC1 salt, 17.8 mg) was prepared from methyl 6-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)pyrazine-2-carboxylate as described for N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide. LRMS (M+H+) m/z calculated 484.94, found 485.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.40 (t, 1 H), 9.03 (s, 1 H), 8.79 (s, 1 H), 7.72 (dd, 1 H), 7.53 (d, 1 H), 7.39 (t, 1 H), 7.36-7.22 (m, 4 H), 7.20 (d, 2H) , 6.38 (d, 1 H), 6.22-6.19 (t, 1 H), 5.03 (s, 2 H), 4.83 (d, 2 H), 4.19 (s, 2 H).
Example 12: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(N-(4-((2-oxopyridin-l
yl)methy
Figure imgf000062_0001
To a solution of ethyl 3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)benzoate (230 mg, 0.66 mmol, 1.0 eq) in DMF (5 mL) was added NaH (79 mg, 60% in oil, 1.98 mmol, 3.0 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 15 min, then acetyl chloride (518 mg, 6.6 mmol, 10 eq) was added into the mixture. The reaction mixture was stirred at rt overnight. The reaction mixture was poured into ice-water (10 mL), extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated under reduced pressure to afford ethyl 3-(N-(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)ac
Figure imgf000062_0002
To a solution of ethyl 3-(N-(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)-acetamido)benzoate (150 mg, 0.38 mmol, 1.0 eq) in MeOH (1 mL) and H2O (1 mL) was added NaOH (46 mg, 1.15 mmol, 3.0 eq). The mixture was heated at 60 °C for 30 min. The mixture was concentrated to remove MeOH, then diluted with ice-water (10 mL) and acidified with 1 N HC1 to pH 3~4. The mixture was concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (20 mL) and filtered. The filtrate was concentrated under reduced pressure to afford 3-(N-(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)acetamido)benzoic acid as a yellow solid (300
Figure imgf000063_0001
To a solution of 3-(N-(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)acetamido)benzoic acid (300 mg, 0.38 mmol, 1.0 eq) in DMF (3 mL) were added DIPEA (147 mg, 1.14 mmol, 3.0 eq) and HATU (173 mg, 0.46 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l-(tetrahydro-2H-pyran- 2-yl)-lH-indazol-3-yl)methanamine (111 mg, 0.42 mmol, 1.1 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)-3-(N-(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)acetamido)benzamide as a white solid (100 mg, 43%).
Figure imgf000063_0002
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-(N-(4-((2-oxopyridin- l(2H)-yl)methyl)phenyl)acetamido)benzamide (100 mg, 0.16 mmol, 1.0 eq) in MeOH (2 mL) was added HC1/EA (2 mL, 4 mmol) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL), neutralized with saturated NaHC03 (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-(N-(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)acetamido)benzamide as a white solid (70 mg, 83%). LRMS (M+H+) m/z caculated 526.2, found 526.2. ¾ NMR (DMSO-ifc, 300 MHz) δ 13.06 (brs, 1 H), 9.10-9.20 (m, 1 H), 7.90 (d, 1 H), 7.75-7.85 (m, 3 H), 7.20-7.60 (m, 9 H), 6.40 (d, 1 H), 6.20-6.30 (m, 1 H), 5.08 (s, 2 H), 4.76 (d, 2 H), 1.92 (s, 3 H).
Example 13: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-l -(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-lH-indole-3-carboxamide
Figure imgf000064_0001
A/"((5"ch|oro"i H"indazo|~3~y|)methy|)"l "(4"((2"oxopyridin"i (2H)"yl)methy|)benzy|) "i H~indO|e~3~cai"boxamide
Figure imgf000064_0002
A mixture of methyl lH-indole-3-carboxylate (263.5 mg, 1.5 mmol, 1.0 eq), l-(4-
(bromomethyl)benzyl)pyridin-2(lH)-one (417.5 mg, 1.5 mmol, 1.0 eq) and K2CO3 (840 mg, 6 mmol, 4 eq) in DMF (5 mL) was stirred at 130 °C overnight. The mixture was mixed with water and extracted with DCM (20 mL X 3). The combined organic layers were dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford methyl l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-indole- 3-carboxylate as a yellow solid (306 mg, 54.8%).
Figure imgf000064_0003
To a solution of methyl l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-indole-3-carboxylate (306 mg, 0.82 mmol, 1.0 eq) in MeOH (10 mL) and THF (10 mL) was added a NaOH aqueous solution (4 N, 10 mL). The mixture was stirred at rt overnight. The organic solvents were evaporated. The resulting residue was acidified to pH 5 with HC1 and extracted with DCM (20 mL X 5). The combined organic layers were dried over anhydrous Na2S04 and concentrated to afford l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-indole-3- carboxylic acid as a yellow solid (290 mg, 98.6%).
Figure imgf000065_0001
A mixture of l -(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)-lH-indole-3-carboxylic acid (72 mg, 0.2 mmol, 1.0 eq), (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (64 mg, 0.24 mmol, 1.2 eq), HATU (1 15 mg, 1.5 mmol, 1.5 eq) and DIPEA (0.8 mL) in DMF (5 mL) was stirred at 60 °C overnight. The mixture was mixed with water and extracted with DCM (10 mL X 3). The combined organic layers were dried over anhydrous Na2SC>4 and concentrated. The residue was purified via prep-TLC (DCM/MeOH = 15/1 , v/v) to afford N-((5-chloro -(tetrahydro-2H-pyran-2-yl) H-indazol-3-yl)methyl) -(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)-lH-indole-3-carboxamide as a yellow solid (50 mg, 41%).
Figure imgf000065_0002
A solution of N-((5-chloro -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-l -(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)-lH-indole-3-carboxamide (50 mg, 0.083 mmol, 1 eq) in MeOH (2 mL) and HCl/dioxane (5 N, 2 mL) was stirred at rt overnight, then concentrated. The residue was suspended in NaHCC solution and extracted with DCM (10 mL X 3). The combined organic layers were dried over anhydrous Na2S04 and concentrated. The residue was purified via prep-TLC (DCM/MeOH = 15/1 , v/v) to afford N-((5-chloro-lH- indazol-3-yl)methyl)-l -(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)-lH-indole-3-carboxamide as a white powder (27 mg, 62.6%). LRMS (M+H+) m/z calculated 522.2, found 521.9. ¾ NMR (CD3OD, 400 MHz) δ 8.20-8.18 (m, 1 H), 7.95-7.94 (m, 2 H), 7.65 (dd, 1 H), 7.54-7.49 (m, 2 H), 7.40-7.34 (m, 2 H) , 7.27-7.25 (m, 2 H) , 7.23-7.18 (m, 4 H) , 6.56 (d, 1 H) , 6.380 (t, 1 H), 5.42 (s, 2 H), 5.16 (s, 2 H), 4.94 (s, 2 H). Example 14: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-l -((4-((2-oxopyridin-l (2H)- yl)methyl)phenyl)sulfonyl)pyrrolidine-3-carboxamide
Figure imgf000066_0001
W"((5"ch|oro"i H indazo 3"y|)methyl)"l ~((4~((2~oxopyrjdin~i (2H)"yl)met yl)pheny|)SU| ony|)pyrro|i,
carboxamjde
Figure imgf000066_0002
To a solution of SOCb (8.2 g, 70 mmol, 2.0 eq) in MeOH (80 mL) at 0 °C was added pyrrolidine-3-carboxylic acid (4 g, 35 mmol, 1.0 eq). The reaction mixture was stirred at 40 °C overnight, and then concentrated to afford methyl pyrrolidine-3 -c
Figure imgf000066_0003
To a solution of 4-(bromomethyl)benzene-l -sulfonyl chloride (1.15 g, 4.26 mmol, 1.1 eq) in DCM (20 mL) was added TEA (1.17 g, 11.6 mmol, 3.0 eq). The reaction mixture was cooled to 0 °C, methyl pyrrolidine-3 - carboxylate (0.5 g, 3.88 mmol, 1.0 eq) was then added. The reaction mixture was stirred at 0 °C for 0.5 h. The mixture was poured into water (20 mL) and extracted with EA (15 mL X 3). The organic layers were washed with brine (10 mL), dried over Na2S04 and concentrated. The residue was purified on silica gel column (PE/EA = 8/1 to 4/1, v/v) to afford methyl l-((4-(bromomethyl)phenyl)sulfonyl)pyrrolidine-3-carboxylate as a yellow solid (300 mg, 21%
Figure imgf000066_0004
To a solution of methyl l-((4-(bromomethyl)phenyl)sulfonyl)pyrrolidine-3 -carboxylate (210 mg, 0.58 mmol, 1.0 eq) in CH3CN (10 mL) were added K2CO3 (241 mg, 1.75 mmol, 3.0 eq) and pyridin-2(lH)-one (55 mg, 0.58 mmol, 1.0 eq). The mixture was stirred at 70 °C for 2 h. The mixture was poured into water (20 mL) and extracted with EA (15 mL X 3). The organic layers were washed with brine (10 mL), dried over Na2S04 and concentrated. The residue was purified on silica gel column (DCM/MeOH = 100/2, v/v) to afford methyl 1 - ((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)pyrrolidine-3-carboxylate as a white solid (160 mg, 73%).
Figure imgf000067_0001
To a solution of methyl l-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)pyrrolidine-3-carboxylate (180 mg, 0.48 mmol, 1.0 eq) in THF/H20 (6 mL/2 mL) was added LiOH (200 mg, 4.8 mmol, 3.0 eq). The mixture was stirred at rt overnight then concentrated to afford l-((4-((2-oxopyridin-l(2H)- yl)methyl)ph
To a solution of l-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)pyrrolidine-3-carboxylic acid (173 mg, 0.5 mmol, 1.0 eq) in DMF (10 mL) were added TEA (150 mg, 1.5 mmol, 3.0 eq), (5-chloro-l-(tetrahydro- 2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (132 mg, 0.5 mmol, 1.0 eq) and HATU (285 mg, 0.75 mmol, 1.5 eq). The reaction mixture was stirred at rtrt overnight. The mixture was mixed with water and extracted with EA. The EA extract was dried over Na2S04 and concentrated. The residue was purified by Prep-HPLC to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-l -((4-((2-oxopyridin-l(2H)- yl)methyl)ph
Figure imgf000067_0003
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-l-((4-((2-oxopyridin- l(2H)-yl)methyl)phenyl)sulfonyl)pyrrolidine-3-carboxamide (60 mg, 0.1 mmol, 1.0 eq) in MeOH (3 mL) was added HCl/MeOH (3 mL, 12 mmol). The mixture was stirred at rt overnight and then concentrated. The residue was purified by prep-HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-l -((4-((2-oxopyridin- l(2H)-yl)methyl)phenyl)sulfonyl)pyrrolidine-3-carboxamide as a white solid (20 mg, 40%>). LRMS (M+H+) m/z calculated 526.01, found 526.1. ¾ NMR (DMSO-tfc, 300 MHz) δ 8.50-8.51 (m, 1H), 7.81-7.84 (m, 2H), 7.73-7.76 (m, 2H), 7.54-7.46 (m, 4H), 7.34-7.37 (m, 1H), 6.56-6.60 (d, 1H), 6.39-6.44 (t, 1H), 5.29 (s, 2H), 4.62-4.85 (m, 2H), 3.50-3.56 (m, 1H), 3.31-3.33 (m, 3H), 2.84-2.89 (m, 1H), 1.92-1.96 (m, 2H).
Example 15: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)piperazine-2-carboxamide
Figure imgf000068_0001
N ((5 chioro 1 H indazoi 3 yl^methyl) 4 (4 ((2 oxopyridin 1 (2H) yl)methyl)benzy|)pjperazjne 2 carboxamide
Figure imgf000068_0002
To a solution of l-(4-(bromomethyl)benzyl)pyridin-2(lH)-one (600 mg, 2.15 mmol, 1.0 eq) in CH3CN (5 mL) were added K2CO3 (0.89 g, 6.45 mmol, 3.0 eq) and 1-tert-butyl 2-methyl piperazine-l,2-dicarboxylate (1.05 g, 4.32 mmol, 2.0 eq). The mixture was heated at 80 °C overnight, and then concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford 1-tert-butyl 2-methyl 4-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)p
Figure imgf000068_0003
To a solution of 1 -tert-butyl 2-methyl 4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l,2- dicarboxylate (150 mg, 0.34 mmol, 1.0 eq) in MeOH (5 mL) and H20 (5 mL) was added NaOH (41 mg, 1.02 mmol, 3.0 eq). The mixture was heated at 60 °C for 30 min. The mixture was concentrated to remove MeOH, then diluted with ice-water (10 mL) and acidified with 1 N HC1 to pH 5-6. The mixture was concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (20 mL) and filtered. The filtrate was concentrated under reduced pressure to afford l-(tert-butoxycarbonyl)-4-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)piperazine-2-carboxylic acid as a white solid (300 mg crude, quant.).
Figure imgf000069_0001
To a solution of l-(tert-butoxycarbonyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-2-carboxylic acid (300 mg crude, 0.34 mmol, 1.0 eq) in DMF (5 mL) were added DIPEA (132 mg, 1.02 mmol, 3.0 eq) and HATU (155 mg, 0.41 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l- (tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (99 mg, 0.37 mmol, 1.1 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford tert-butyl 2-(((5- chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)carbamoyl)-4-(4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl
Figure imgf000069_0002
To a solution oftert-butyl 2-(((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)carbamoyl)-4- (4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l-carboxylate (100 mg, 0.15 mmol, 1.0 eq) in MeOH (2 mL) was added HC1/EA (2 mL, 4 mmol) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL), neutralized with saturated NaHCC (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)piperazine-2-carboxamide as a white solid (20 mg, 27%). LRMS (M+H+) m/z caculated 491.2, found 491.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.02 (brs, 1 H), 8.30-8.40 (m, 1 H), 7.85 (s, 1 H), 7.77 (d, 1 H), 7.51 (d, 1 H), 7.40-7.48 (m, 1 H), 7.33 (d, 1 H), 7.10-7.30 (m, 4 H), 6.41 (d, 1 H), 6.23 (t, 1 H), 5.06 (s, 2 H), 4.50-4.60 (m, 2 H), 3.30-3.45 (m, 4 H), 3.20-3.25 (m, 1 H), 2.70-2.85 (m, 1 H), 2.55-2.68 (m, 2 H), 1.90-2.00 (m, 2 H). Example 16: Preparation of methyl 2-(5-chloro-3-((l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH- pyrazole-4-carboxamido)methyl)-lH-indazol-l -yl)acetate methyl 1 H dazoM
Figure imgf000070_0001
The mixture of N-((5-chloro- 1 H-indazol-3 -yl)methyl)- 1 -(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)- 1 H- pyrazole-4-carboxamide (94 mg, 0.2 mmol, 1.0 eq), methyl 2-chloroacetate (62 mg, 0.57 mmol, 2.9 eq) and CS2CO3 (327 mg, 1 mmol, 5.0 eq) in DMF (3 mL) was stirred at 50 °C overnight. The mixture was poured into water and the precipitate was collected by suction and purified via prep-TLC (DCM:MeOH = 20:1, v/v) to afford methyl 2-(5-chloro-3-((l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)methyl)-lH-indazol-l-yl)acetate as a white powder (56 mg, quant.). LRMS (M+H+) m/z calculated 545.2, found 545.2. ¾ NMR (CD3OD, 400 MHz) δ 8.15 (s, 1 H), 7.95 (s, 1 H), 7.88 (d, 1 H), 7.70 (dd, 1 H), 7.56-7.54 (m, 1 H), 7.51 (d, 1 H), 7.40 (dd, 1 H), 7.32 (d, 2 H), 7.27 (d, 2 H), 6.58 (d, 1 H) , 6.40 (t- d, 1 H), 5.36 (s, 2 H), 5.27 (s, 2 H), 5.20 (s, 2 H), 4.84 (s, 2 H), 3.77 (s, 3 H).
Example 17: Preparation of 2-(5-chloro-3-((l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)methyl)-lH-indazol-l -yl)acetic acid
I
Figure imgf000070_0002
2 (5 chioro 3 (4 oxopyndin <| (2H) yl)methyl)benzyK <| H pyrazo|e 4 carboxamido^methyl) 1 H indazoi 1
yl^acetic acjd
Figure imgf000071_0001
The mixture of methyl 2-(5-chloro-3-((l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)methyl)-lH-indazol-l-yl)acetate (230 mg, 0.42 mmol, 1.0 eq) and NaOH (34 mg, 0.85 mmol, 2.0 eq) in H20 (1.5 mL), THF (1.5 mL) and MeOH (1.5 mL) was stirred at 25 °C overnight. The mixture was neutralized with 1 N HCl and the precipitate was collected by suction to afford 2-(5-chloro-3-((l -(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4-carboxamido)methyl)-lH-indazol-l -yl)acetic acid as a yellow solid (160 mg, 72%). LCMS (M-H+) m/z calculated 529.14, found 528.8. ¾ NMR (DMSO-d6, 300 MHz) δ 13.12 (brs, 1H), 8.75 (t, 1H), 8.27 (s, 1H), 7.90 (s, 2H), 7.73 (dd, 1H), 7.65 (d, 1H), 7.41-7.37 (m, 2H), 7.26-7.19 (m, 4H), 6.38 (d, 1H), 6.21 (t, 1H), 5.29 (s, 2H), 5.23 (s, 2H), 5.05 (s, 2H), 4.66 (d, 2H).
Example 18: Preparation of N-((l-(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-l-(4-((2- oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H-pyrazole-4-carboxamide
Figure imgf000071_0002
The mixture of 2-(5-chloro-3-((l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)methyl)-lH-indazol-l-yl)acetic acid (80 mg, 0.15 mmol, 1.0 eq), HATU (86 mg, 0.23 mmol, 1.5 eq), triethylamine (75 mg, 0.75 mmol, 5.0 eq) and NH4Cl (16 mg, 0.30 mmol, 2.0 eq) in DMF (3 mL) was stirred at 25 °C overnight. The mixture was poured into water and the precipitate was collected by suction and purified via prep-HPLC to afford N-((l-(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-l -(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4-carboxamide as a white powder (5.4 mg, 7%). LCMS (M+H+) m/z calculated 530.17, found 530.2. ¾ NMR (DMSO-d6, 300 MHz) δ 8.72 (t, 1H), 8.27 (s, 1H), 7.89 (s, 1H), 7.88 (d, 1H), 7.74 (dd, 1H), 7.61-7.58 (m, 2H), 7.43-7.35 (m, 2H), 7.29-7.19 (m, 4H), 6.38 (d, 2H), 6.21 (td, 1H), 5.29 (s, 2 H), 5.05 (s, 2H), 5.01 (s, 2H), 4.66 (d, 2H).
Example 19: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-oxo-4-(4-((2-oxopyridin-l(2H)- yl)meth l benz l -4 5-dih dro razine-2-carboxamide
Figure imgf000072_0001
car oxamjde
Figure imgf000072_0002
A mixture of l-(4-(bromomethyl)benzyl)pyridin-2(lH)-one (200 mg, 0.719 mmol, 1.0 eq), methyl 5-oxo-4,5- dihydropyrazine-2-carboxylate (133 mg, 0.863 mmol, 1.2 eq), K2CO3 (198 mg, 1.43 mmol, 2.0 eq) in MeCN (5 mL) was stirred at 85 °C under N2 overnight and concentrated. The residue was mixed with water (20 mL), extracted with EA (30 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via flash chromatography (MeCN/EhO = 30 %-95 %, v/v) to afford methyl 5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-4,5-dihydropyrazine-2-carboxylate as a white solid (169 mg, 66%).
Figure imgf000072_0003
To a solution of methyl 5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-4,5-dihydropyrazine-2- carboxylate (160 mg, 0.455 mmol, 1.0 eq) in MeOH (10 mL) was added drop wise a solution of NaOH (182 mg, 4.55 mmol, 10.0 eq) in water (5 mL). The mixture was stirred at rt overnight. The organic solvents were evaporated. To the residue was added cone. HCl till pH 3; the precipitate was collected and dried to afford 5- oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-4,5-dihydropyrazine-2-carboxylic acid (126 mg, 82%).
Figure imgf000073_0001
A mixture of 5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-4,5-dihydropyrazine-2-carboxylic acid (120 mg, 0.356 mmol, 1.0 eq), (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (94 mg, 0.356 mmol, 1.0 eq), HATU (149 mg, 0.391 mmol, 1.1 eq) and DIEA (92 mg, 0.712 mmol, 2 eq) in DMF (3 mL) was stirred at rt for 3 hours. The mixture was poured into aqueous saturated NaHCCb (30 mL), extracted with EA (30 mL X 2). The combined organic layers were washed with brine, dried over sodium sulfate, filtered and concentrated in vacuo. The residue was purified by flash chromatography to afford N-((5-chloro- l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)- 4,5-dihydropyrazine-2-carboxamide as a white solid (116 mg, 55%).
Figure imgf000073_0002
A solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-oxo-4-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)-4,5-dihydropyrazine-2-carboxamide (110 mg, 0.188 mmol, 1 eq) in MeOH (5 mL) and HCl/Et20 (3 N, 5 mL) was stirred at rt overnight. The precipitate was collected and dried to afford the HC1 salt ofN-((5-chloro-lH-indazol-3-yl)methyl)-5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-4,5- dihydropyrazine-2-carboxamide as a white solid (23 mg, 22%). LRMS (M+H+) m/z calculated 501.2, found 501.2. 'H NMR (DMSO-ifc, 300 MHz) δ 13.02 (s, 1 H), 9.01-8.99 (m, 1 H), 8.51 (s, 1 H), 8.01-7.99 (m, 2 H), 7.76-7.74 (m, 1 H), 7.53-7.49 (m, 1 H), 7.47-7.23 (m, 6 H), 6.38 (d, 1 H), 6.31 (t, 1 H), 5.14 (s, 2 H), 5.05 (s, 2 H), 4.73 (d, 2 H). Example 20: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-6-oxo-l-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-l,6-dihydropyridine-3-carboxamide
Figure imgf000073_0003
N ((5 chioro 1 H indazoi 3 yKmethyh 6 oxo 1 ,4 , oxopyridin 1 (2H) yhmethyhbenzyh ι ·6 dihydropyndine 3
carhoxamide
Figure imgf000074_0001
A mixture of methyl 6-oxo-l,6-dihydropyridine-3-carboxylate (115 mg, 0.75 mmol, 1.05 eq), l-(4- (bromomethyl)benzyl)pyridin-2(lH)-one (198 mg, 0.71 mmol, 1.0 eq) and K2CO3 (220 mg, 1.6 mmol, 2.2 eq) in MeCN (5 mL) was stirred at 85 °C overnight. The mixture was filtered and the filtrate was concentrated. The residue was purified via flash chromatography to afford methyl 6-oxo-l-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-l,6
Figure imgf000074_0002
To a solution of methyl 6-oxo-l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-l,6-dihydropyridine-3- carboxylate (214 mg, 0.61 mmol, 1.0 eq) in MeOH (10 mL) and THF (10 mL) was added a 2 N NaOH aqueous solution (10 mL). The mixture was stirred at rt overnight. The organic solvents were evaporated. The residue was adjusted to pH 2 with HC1 and filtered. The solid was collected to afford 6-oxo- 1-(4-((2- oxopyri 97.6%).
Figure imgf000074_0003
A mixture of 6-oxo-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-l,6-dihydropyridine-3-carboxylic acid (85 mg, 0.25 mmol, 1.0 eq), (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (80 mg, 0.30 mmol, 1.2 eq), HATU (124 mg, 0.32 mmol, 1.3 eq) and DIPEA (1 mL) in DMF (4 mL) was stirred at 55 °C overnight. The mixture was concentrated and the residue was purified via flash chromatography to afford N- ((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-6-oxo-l-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-l,6-dihydropyridine-3-carboxamide as a yellow solid (115 mg, 78.2%>).
Figure imgf000075_0001
A solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-6-oxo-l -(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)-l,6-dihydropyridine-3-carboxamide (115 mg, 0.197 mmol, 1 eq) in MeOH (3 mL) and HCl/dioxane (5 N, 3 mL) was stirred at rt overnight, then diluted with DCM (10 mL). The mixture was filtered and the solid was washed with DCM to afford the HC1 salt of N-((5-chloro-lH-indazol-3-yl)methyl)- 6-oxo-l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-l,6-dihydropyridine-3-carboxamide as a yellow solid (84 mg, 79.5%). LRMS (M+H+) m/z calculated 500.1, found 500.2. ¾ NMR (CD3OD, 300 MHz) δ 8.42-8.40 (m, 1 H), 8.00-7.94 (m, 2 H), 7.87-7.86 (m, 1 H), 7.84-7.78 (m, 1 H), 7.51 (d, 1 H) , 7.40-7.31 (m, 5 H), 6.83 (d, 1 H) , 6.75 (t-d, 1 H), 6.57 (d, 1 H) , 5.33 (s, 2 H), 5.22 (s, 2 H), 4.87 (s, 2 H).
Example 21: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)picolinamide
Figure imgf000075_0002
A mixture of l-(4-((4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)methyl)benzyl)pyridin-2(lH)-one (200 mg, 0.61 mmol, 1.0 eq), ethyl 6-bromopicolinate (170 mg, 0.73 mmol, 1.2 eq), Na2C03 (196 mg, 1.8 mmol, 3.0 eq) and Pd(PPh3)4 (71 mg, 0.06 mmol, 0.1 eq) in dioxane/H20 (20 mL/2 mL) was stirred at 95 °C under N2 overnight. After cooled to rt, the mixture was filtered. The filtrate was concentrated. The residue was purified on silica gel column (PE/EtOAc = 1/1) to afford ethyl 6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)picolinate as a yellow oil (62 mg, 28%).
Figure imgf000076_0001
To a solution of ethyl 6-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)picolinate (45 mg, 0.12 mmol, 1.0 eq) in MeOH (2 mL) and H2O (1 mL) was added L1OH.H2O (16 mg, 0.38 mmol, 3.0 eq). The mixture was stirred at 70 °C for 2 h. The organic solvents were evaporated. The residue was acidified with cone HCl to pH 5 and extracted with EA (3x20 mL), the combined organic layer was washed with brine, dried over Na2S04, filtered and concentrated in vacuo to afford 6-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)picolinic acid as a white solid (32 mg, 77%).
Figure imgf000076_0002
A mixture of 6-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)picolinic acid (32 mg, 0.10 mmol, 1.0 eq), (5- chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (31.8 mg, 0.12 mmol, 1.2 eq), HATU (46 mg, 0.12 mmol, 1.2 eq) and DIEA (39 mg, 0.30 mmol, 3.0 eq) in DMF (2 mL) was stirred at rt for 3 h. The mixture was purified by flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)picolinamide as a white solid (21 mg, 37%).
Figure imgf000076_0003
A solution of N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-6-(4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)picolinamide (21 mg, 0.03 mmol, 1.0 eq) in EA (5 mL) and HCl/Et20 (3 N, 2 mL) was stirred at rt for 20 h. Then the mixture was evaporated under reduced pressure to afford N-((5-chloro-lH- indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)picolinamide as a white solid (1 1 mg, 57%). LRMS (M+H+) m/z calculated 484.1 , found 484.1. ¾ NMR (MeOH-d4, 400 MHz) δ 7.84 (d, 1H), 7.77 (d, 1H), 7.72 (t, 1H), 7.51 (m, 1H), 7.41 -7.37 (m, 2H), 7.26-7.22 (m, 2H), 7.16 (d, 2H), 7.09 (d, 2H), 6.44 (d, 1H), 6.24 (t, 1H), 5.02 (s, 2H), 4.83 (s, 2H), 4.05 (s, 2H). Example 22: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000077_0001
N~((5~chloro~i H~indazo| y|)methy|)^
Figure imgf000077_0002
A mixture of l -(4-((4,4,5,5-tetramethyl-l ,3,2-dioxaborolan-2-yl)methyl)benzyl)pyridin-2(lH)-one (200 mg, 0.61 mmol, 1.0 eq), methyl 2-bromoisonicotinate (159 mg, 0.73 mmol, 1.2 eq), Na2C03 (196 mg, 1.8 mmol, 3.0 eq) and Pd(PPh3)4 (71 mg, 0.06 mmol, 0.1 eq) in dioxane/H20 (20 mL/2 mL) was stirred at 95 °C under N2 overnight. After cooled to rt, the mixture was filtered. The filtrate was concentrated. The residue was purified on silica gel column (PE/EtOAc = 1/1) to afford methyl 2-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)is
Figure imgf000077_0003
To a solution of methyl 2-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)isonicotinate (70 mg, 0.20 mmol, 1.0 eq) in MeOH (6 mL) and H2O (4 mL) was added NaOH (82 mg, 2.0 mmol, 10.0 eq). The mixture was stirred at 70 °C for 1 h. The organic solvents were evaporated. The residue was acidified with cone HC1 to pH 5 and extracted with EA (3x20 mL), the combined organic layer was washed with brine, dried over Na2S04, filtered and concentrated in vacuo to afford 2-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)isonicotinic acid as a white solid (64 mg, 95%).
Figure imgf000078_0001
A mixture of 2-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)isonicotinic acid (64 mg, 0.19 mmol, 1.0 eq), (5- chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (63 mg, 0.23 mmol, 1.2 eq), HATU (82 mg, 0.21 mmol, 1.1 eq) and DIEA (51 mg, 0.39 mmol, 2.0 eq) in DMF (1 mL) was stirred at rt overnight. The mixture was poured into water (25 mL), extracted with EA (2x40 mL). The combined organic layer was washed with brine, dried over Na2S04, filtered and concentrated in vacuo. The residue was then further purified by flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)-2-( -((2-oxopyridin-l (2H)-yl)methyl)benzyl)isonicotinamide as a brown oil (70 mg, 61%).
Figure imgf000078_0002
A solution of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)isonicotinamide (70 mg, 0.12 mmol, 1.0 eq) in EA (5 mL) and HCl/Et20 (5 N, 5 mL) was stirred at rt for 20 h. Then the mixture was concentrated. The residue was purified by flash chromatography (MeCN/H20 = 1/2 to 9/1) to afford N-((5-chloro-l H-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)isonicotinamide as a white solid (21 mg, 35%). LRMS (M+H+) m/z calculated 484.1 , found 484.1. 'H NMR (MeOH-d4, 400 MHz) δ 8.43 (d, 1H), 7.73 (s, 1H), 7.54-7.48 (m, 3H), 7.39-7.34 (m, 2H), 7.22 (d, 1H), 7.11 (m, 4H), 6.43 (d, 1H) , 6.23 (t, 1H), 5.01 (s, 2H), 4.76 (s, 2H), 4.03 (s, 2H).
Example 23: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l (2H)- yl)methyl)phenoxy)nicotinamide
Figure imgf000078_0003
N ((5 chioro 1 H indazoi 3 yljmethyl) 5 (4 ((2 oxopyHdin 1 (2H) y|)methyl)phenoXy)niCOtinamic|e
Figure imgf000079_0001
A mixture of 5-hydroxynicotinic acid (3 g, 21.6 mmol), cone H2SO4 (2.2 g, 21.6 mmol) in MeOH (50 mL) was heat at 70 °C overnight. The mixture was concentrated, poured into water (100 mL) and extracted with EA (100 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated to afford methyl 5-hydro
Figure imgf000079_0002
A mixture of methyl 5-hydroxynicotinate (2 g, 0.01 mol), 4-fluorobenzaldehyde (2.1 g, 0.13 mol) and K2CO3 (3.6 g, 0.02 mol) in DMF (50 mL) was heated at 70 °C for overnight. The mixture was diluted with water (100 mL) and extracted with EA (100 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. Purification by flash chromatography (PE/EA = 6/1, v/v) afforded methyl 5-(4- formylphenoxy)nicotinate as a yellow solid (2.2 g, 65%).
Figure imgf000079_0003
A mixture of methyl 5-(4-formylphenoxy)nicotinate (1.5 g, 5.76 mmol) and NaBFL (218 mg, 5.76 mmol) in MeOH (20 mL) was stirred at rt for 10 min. The reaction was quenched with aq NH4CI (10 mL). The mixture was concentrated, poured into water (100 mL) and extracted with EA (100 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. Purification by flash chromatography (PE/EA = 1/1, v/v) afforded methyl 5-(4-(hydroxymethyl)phenoxy)nicotinate as a yellow solid (1.48 g, 64%>).
Figure imgf000079_0004
A solution of methyl 5-(4-(hydroxymethyl)phenoxy)nicotinate (500 mg, 1.92 mmol), ΡΙ¾ (785 mg, 2.90 mmol) in DCM (5 mL) was stirred at rt for 2 h. The mixture was diluted with water (100 mL) and extracted with EA (100 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated to afford methy %).
Figure imgf000080_0001
A mixture of methyl 5-(4-(bromomethyl)phenoxy)nicotinate (630 mg, 2.3 mmol), pyridin-2(lH)-one (238 mg, 2.5 mmol) and K2CO3 (952 mg, 7 mmol) in CH3CN (10 mL) was heated at 80 °C for overnight. The mixture was diluted with water (100 mL) and extracted with EA (100 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. Purification by flash chromatography (PE/EA = 1/1, v/v) afforded methyl 5- , 30%).
Figure imgf000080_0002
A mixture of methyl 5-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)nicotinate (90 mg, 0.34 mmol) and NaOH (200 mg, 0.51 mmol) in THF/H2O (4 mL / 1 mL) was stirred at rt for 2 h. The mixture was concentrated to afford 5-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)nicotinic acid (50 mg, 80%) which was used directly withtout furthe
Figure imgf000080_0003
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)nicotinic acid (40 mg, 0.12 mmol), (5-chloro-l- (tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (33 mg, 0.12 mmol) and TEA (38 mg, 0.37 mmol), HATU (71 mg, 0.18 mmol) in DMF (5 mL) was stirred at rt for 2 h. The mixture was diluted with water (100 mL) and extracted with EA (100 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. Purification by flash chromatography (PE/EA = 1/1, v/v) afforded N-((5-chloro-l- (tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-5-(4-((2-oxopyridin- 1 (2H)- yl)methyl)phenoxy)nicotinamide as a white solid (50 mg, 73%).
Figure imgf000081_0001
A mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)phenoxy)nicotinamide (50 mg, 0.09 mmol) in HCl/MeOH (5 mL) was stirred at rt overnight. The mixture was concentrated and purified by prep-HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4- ((2-oxopyridin-l(2H)-yl)methyl)phenoxy)nicotinamide as a white solid (13 mg, 31%). LRMS (M+H+) m/z calculated 486, found 486.1. ¾ NMR (CD3OD, 400 MHz) δ 9.12 (s, 1 H), 8.81 (s, 1 H), 8.58 (s, 1 H), 8.14- 8.13 (d, 1 H), 7.92 (s, 1 H), 7.91-7.89 (m, 1 H ), 7.57-7.53 (m, 3 H), 7.42-7.39 (m, 1 H), 7.31-7.29 (d, 2 H), 6.92-6.90 (d, 1 H), 6.85-6.82 (m, 1 H ), 5.42 (s, 2 H ), 4.96 (s, 2 H ). Example 24: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)- yl)methyl)phenoxy)benzamide
Figure imgf000081_0002
N ((5 chioro 1 H indazoi 3 yl^methyl) 3 (4 ((2 oxopyrjdin -| (2H) y|)methyl)phenoxy)benzamjde
Figure imgf000081_0003
The mixture of methyl 3-hydroxybenzoate (2 g, 13.2 mmol, 1.0 eq), 4-fluorobenzaldehyde (1.6 g, 13.2 mmol, 1.0 eq) and K2C03 (3.6 g, 26.4 mmol, 2.0 eq) in CH3CN (50 mL) was stirred at 80 °C overnight. Then the mixture was cooled to rt and filtered. The filtrate was concentrated. The residue was purified on silica gel column (PE/EtOAc = 5/1) to afford methyl 3-(4-formylphenoxy)benzoate as a colorless oil (1.7 g, 50%).
Figure imgf000082_0001
To a solution of methyl 3-(4-formylphenoxy)benzoate (1.7 g, 6.64 mmol, 1.0 eq) in MeOH (20 mL) was added NaBH4 (505 mg, 13.3 mmol, 2.0 eq) at 0 °C. The mixture was stirred at rt for 2 h. LCMS showed the reaction complete. Then the mixture was concentrated, diluted with EtOAc (50 mL). The mixture was washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified on silica gel column (PE/EtOAc = 5/1) to give methyl 3-(4-(hydroxymethyl)phenoxy)benzoate as a yellow oil (680 mg, 40%).
Figure imgf000082_0002
The mixture of methyl 3-(4-(hydroxymethyl)phenoxy)benzoate (680 mg, 2.6 mmol, 1.0 eq) in SOCh. (10 mL) was refluxed for 2 h. Then the mixture was concentrated. The residue was diluted with EtOAc (50 mL). The mixture was washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was dried to afford methyl 3-(4- mg, 86%).
Figure imgf000082_0003
A mixture of methyl 3-(4-(chloromethyl)phenoxy)benzoate (300 mg, 1.08 mmol, 1.0 eq), pyridin-2(lH)-one (124 mg, 1.30 mmol, 1.2 eq) and K2C03 (447 mg, 3.24 mmol, 3.0 eq) in CH3CN (10 mL) was stirred at 80 °C overnight. After cooled to rt, the mixture was filtered. The filtrate was concentrated. The residue was purified on silica gel column (PE/EtOAc = 1/1) to afford methyl 3-(4-((2-oxopyridin-l(2H)- yl)methyl)phenoxy)b
Figure imgf000082_0004
To a solution of methyl 3-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)benzoate (300 mg, 0.9 mmol, 1.0 eq) in MeOH (5 mL) and H2O (5 mL) was added NaOH (107 mg, 2.7 mmol, 3.0 eq). The mixture was stirred at rt for 1 h. The organic solvents were evaporated. To the residue was added cone. HCl till pH 2. The precipitate was collected and dried to afford 3-(4-((2-oxopyridin-l (2H)-yl)methyl)phenoxy)benzoic acid as a white solid (250 mg, 86%).
Figure imgf000083_0001
A mixture of 3-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)benzoic acid (250 mg, 0.78 mmol, 1.0 eq), (5- chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (248 mg, 0.93 mmol, 1.2 eq), HATU (593 mg, 1.56 mmol, 2.0 eq) and DIEA (302 mg, 2.34 mmol, 3.0 eq) in DMF (10 mL) was stirred at 60 °C overnight. The mixture was poured into water (50 mL). The precipitate was collected and dried to afford N- ((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)- yl)
Figure imgf000083_0002
A solution of N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-3-(4-((2-oxopyridin- 1 (2H)- yl)methyl)phenoxy)benzamide (420 mg, 0.74 mmol, 1.0 eq) in MeOH (1 mL) and HCl/dioxane (5 N, 5 mL) was stirred at rt for 2 h. Then the mixture was concentrated. The residue was recrystallized with
MeOH/EtOAc to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)- yl)methyl)phenoxy)benzamide as a white solid (20.6 mg, 6%>). LRMS (M+H+) m/z calculated 485.1, found 485.1. 'H NMR (DMSO-ifc, 400 MHz) δ 13.06 (s, 1H), 9.17 (t, 1H), 7.89 (d, 1H), 7.82-7.80 (m, 1H), 7.66 (d, 1H), 7.50-7.30 (m, 7H), 7.17 (s, 1H), 7.01 (d, 2H) , 6.41 (d, 1H), 6.24 (t, 1H), 5.07 (s, 2H), 4.75 (d, 2H).
Example 25: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-6-oxo-l-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)piperidine-3-carboxamide
Figure imgf000084_0001
chioro i n indazoi 3 y^methyl) 6 oxo 1 (4 ((2 oxopyridin 1 (2H) yl)methyl)benzy|)pjperidine 3 carboxamide
Figure imgf000084_0002
To a solution of methyl 6-oxopiperidine-3-carboxylate (113 mg, 0.72 mmol, 1.0 eq) in DMF (2 mL) was added NaH (86 mg, 2.16 mmol, 3.0 eq) at 0 °C under N2. The suspension was stirred at 0 °C for 15 min, then l-(4-(bromomethyl)benzyl)pyridin-2(lH)-one (200 mg, 0.72 mmol, 1.0 eq) was added into the mixture. The mixture was stirred at rt for 2 h. The reaction mixture was poured into ice-water (5 mL), acidified with 1 N HC1 to pH 2-3, and concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford 6-oxo-l-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)piperidine-3-carboxylic acid as a yellow solid (80 mg, 33%).
Figure imgf000084_0003
To a solution of 6-oxo-l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3-carboxylic acid (80 mg, 0.24 mmol, 1.0 eq) in DMF (3 mL) were added DIPEA (93 mg, 0.72 mmol, 3.0 eq) and HATU (109 mg, 0.29 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min. (5 -chioro- 1 -(tetrahy dro-2H-pyran- 2-yl)-lH-indazol-3-yl)methanamine (69 mg, 0.26 mmol, 1.1 eq) was added into the mixture. The mixture was stirred at rt overnight under N2. The mixture was poured into water (10 mL), extracted with EA (10 mL *3); the combined organic layer was washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)-6-oxo-l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3-carboxamide as a yellow solid (120 mg, 85%).
Figure imgf000085_0001
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-6-oxo-l -(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3-carboxamide (120 mg, 0.2 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/EA (2 mL) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL), neutralized with saturated
NaHC03 (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3-yl)methyl)-6-oxo-l-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)piperidine-3-carboxamide as a white solid (70 mg, 69%). LRMS (M+H+) m/z caculated 504.1, found 504.2. ¾ NMR (DMSO-i e, 300 MHz) δ 13.04 (brs, 1 H), 8.59 (t, 1 H), 7.80-7.75 (m, 2 H), 7.52 (d, 1 H), 7.42 (t-d, 1 H), 7.33 (d, 1 H), 7.28-7.12 (m, 4 H), 6.40 (d, 1 H), 6.22 (t, 1 H), 5.06 (s, 2 H), 4.60-4.30 (m, 4 H), 3.28-3.15 (m, 2 H), 2.80-2.60 (m, 1 H), 2.40-2.20 (m, 2 H), 2.00-1.80 (m, 2 H).
Example 26: Preparation of 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(3-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)phenyl)acetic acid
Figure imgf000085_0002
To a solution of methyl 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(3-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)phenyl)acetate (180 mg, 0.31 mmol, 1.0 eq) in MeOH (10 mL) and ¾0 (5 mL) was added NaOH (128 mg, 3.1 mmol, 10.0 eq). The mixture was stirred at 70 °C for 3 h. The organic solvents were evaporated. To the residue was added cone HC1 till pH 3. The precipitation formed was filtered and dried to afford 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(3-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)phenyl)acetic acid as a white solid (62 mg, 38%). LRMS (M+H+) m/z calculated 513.2, found 513.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.13 (s, 1H), 7.91 (s, 1H), 7.74 (d, 1H), 7.53 (m, 1H), 7.41- 7.32 (m, 2H), 7.24-7.10 (m, 8H), 7.08 (d, 1H), 6.38 (d, 1H) , 6.20 (t, 1H), 5.02 (s, 2H), 4.23 (s, 1H), 4.04 (d, 2H), 3.87 (s, 2H).
Example 27: Preparation of methyl 2-(5-chloro-lH-indazole-3-carboxamido)-2-(5-(4-((2-oxopyridin-l yl)methyl)benzyl)pyridin-3-yl)acetate
Figure imgf000086_0001
methyl 2"(5"ch|oro"i H dazo|e"3"carboxamjdO)"2"(5"(4"^
Figure imgf000086_0002
To a stirred solution of 5-bromonicotinaldehyde (4.5 g, 24 mmol, 1.0 eq) in ammonia solution (60 ml, 5 M solution in methanol) was added tetraisopropyl orthotitanate (8.2 g, 29 mmol, 1.2 eq) and the resulting mixture was stirred at r.t. for 2 h. Trimethylsilylcyamde (2.4 g, 24 mmol, 1.0 eq) was then added dropwise and stirring continued at rt for 22 h. The reaction mixture was poured into ice-water (300 mL). After stirring, the resulting mixture was filtered through celite, washing with ethyl acetate (80 mLx3). The filtrate was concentrated in vacuo and the residue was taken up in ethyl acetate and washed sequentially with distilled water and with saturated brine. The organic phase was then dried over sodium sulphate and concentrated in vacuo to give an oil, which was further purified by flash chromatography to afford 2-amino-2-(5- bromopyridin-3-yl)acetonitrile (1.3 g, 25%) as a yellow solid.
Figure imgf000086_0003
To a solution of 2-amino-2-(5-bromopyridin-3-yl)acetonitrile in 10 mL of methanol was added drop wise HCI/dioxane (5 N, 5 mL). The mixture was stirred at rt overnight and then heated to reflux for 3 h. After cooling to rt, the mixture was concentrated under reduced pressure, the residue was poured into saturated NaHC03 (60 mL), extracted with EA (50 mLx 3) and the combined organic layer was washed with brine, dried over Na2S04, filtered and concentrated in vacuo to give methyl 2-amino-2-(5-bromopyridin-3-yl)acetate (1.0 g, 66%) as a yellow oil.
Figure imgf000087_0001
2-Amino-2-(5-bromopyridin-3-yl)acetate (1.0 g, 4.0 mmol, 1.0 eq) was diluted with dichloromethane (60 mL) and slowly added with NaHCCb (685 mg, 8.1 mmol, 2.0 eq), triethylamine (1.0 mL, 8.1 mmol, 2.0 eq), and di- tert-butyl dicarbonate (1.0 g, 4.8 mmol, 1.2 eq) at rt in sequence. The reaction solution was stirred for 2 h at the same temperature, and slowly added with a saturated aqueous solution of ammonium chloride (100 mL). The aqueous layer was extracted twice with ethyl acetate. The organic layer was collected, dried over Na2S04, filtered, and concentrated under reduced pressure. The residue was purified by chromatography on silica gel column (PE/EA = 30/1 to 3/1) to afford methyl 2-(5-bromopyridin-3-yl)-2-((fert- butoxycarb
Figure imgf000087_0002
To a suspension of methyl 2-(5-bromopyridin-3-yl)-2-((tert-butoxycarbonyl)amino)acetate (710 mg, 2.0 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2-dioxaborolane) (523 mg, 2.0 mmol, 1.0 eq) and AcOK (605 mg, 6.1 mmol, 3.0 eq) in dioxane (40 mL) was added Pd(dppf)Ci2 (75 mg, 0.1 mmol, 0.05 eq). The mixture was heated to 85 °C under N2 overnight. After cooled to rt, l-(4-(bromomethyl)benzyl)pyridin- 2(lH)-one (480 mg, 2.0 mmol, 1.0 eq), and a solution of Na2C03 (654 mg, 6.1 mmol, 3.0 eq) in water (6 mL) were added, followed by Pd(dppf)Ch (75 mg, 0.1 mmol, 0.05 eq). The mixture was then heated to 95 °C for a further 2 h and allowed to cool. After removal of the solvent, the residue was poured into water (60 mL), extracted with EA (3x60 mL), the combined organic layer was washed with brine, dried over Na2S04, filtered and concentrated in vacuo to give an oil, which was further purified by chromatography on silica gel column (PE/EA = 2/1 , then DCM/MeOH = 10/1 ) to afford methyl 2-((tert-butoxycarbonyl)amino)-2-(5-(4-((2- oxopyridin-l(2
Figure imgf000087_0003
A solution of methyl 2-((tert-butoxycarbonyl)amino)-2-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridin- 3-yl)acetate (440 mg, 0.95 mmol, 1.0 eq) in MeOH (5 mL) and HC1/EA (3 N, 3 mL) was stirred at rt for 20 h. Then the mixture was concentrated to afford methyl 2-amino-2-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)b
Figure imgf000088_0001
A mixture of 5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carboxylic acid (130 mg, 0.46 mmol, 1.0 eq), methyl 2-amino-2-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate HC1 salt (202 mg, 0.46 mmol, 1.0 eq), HATU (176 mg, 0.46 mmol, 1.0 eq) and DIEA (1 mL) in DCM (30 mL) was stirred at rt for 20 h. The mixture was diluted with DCM (30 mL), washed with saturated NaHCC (20 mL), and the brine, dried over Na2SC>4, filtered and concentrated in vacuo. The residue was then further purified by flash chromatography (MeCN/H20 = 25%- 95%) to afford methyl 2-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH- indazole-3-carboxamido)-2-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate as a white solid (150 mg, 51%).
Figure imgf000088_0002
A solution of methyl 2-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carboxamido)-2-(5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate (50 mg, 0.079 mmol, 1.0 eq) in MeOH (3 mL) and HCl/EA (3 N, 2 mL) was stirred at 40 °C for 5 h. Then the mixture was concentrated. The residue was purified by flash chromatography (MeCN/E O = 1/2 to 5/1) to afford methyl 2-(5-chloro-lH-indazole-3- carboxamido)-2-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate as a white solid (26 mg, 60%). LRMS (M+H+) m/z calculated 542.1, found 542.1. 'H NMR (DMSO-ifc, 300 MHz) δ 13.93 (s, 1H), 9.26 (d, 1H), 8.52 (d, 1H), 8.42 (d, 1H), 8.09 (d, 1H), 7.76-7.68 (m, 3H), 7.46-7.37 (m, 2H), 7.23-7.18 (m, 4H), 6.38 (d, 1H), 6.21 (m, 1H), 5.80 (d, 2H), 5.02 (s, 2H), 3.94 (s, 2H), 3.65 (s, 2H).
Example 28: Preparation of methyl 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(5-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)pyridin-3 -yl)acetate
Figure imgf000089_0001
meth l 2"(((5"ch|oro"i H dazof3"y|)methy|)amino^
Figure imgf000089_0002
A mixture of 5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carbaldehyde (73 mg, 0.27 mmol, 1.0 eq), methyl 2-amino-2-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate HCl salt (100 mg, 0.27 mmol, 1.0 eq) and AcOH (5 drops) in MeOH (3 mL) was stirred at rt for 1 h. NaBH4 (209 mg, 5.5 mmol, 20 eq) was then added portion wise. The mixture was stirred at rt for a further 1 h and then quenched with water (2 mL). After workup, it was purified by flash chromatography (MeCN/l hO = 1/3 to 10/1) to afford methyl 2- (((5-chloro -(tetrahydro-2H-pyran-2-yl) H ndazol-3-yl)methyl)amino)-2-(5-(4-((2-oxopyridin-l(2H)- yl)
Figure imgf000089_0003
A solution of methyl 2-(((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)amino)-2-(5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate (40 mg, 0.065 mmol, 1.0 eq) in MeOH (2 mL) and HCl/EA (3 N, 3 mL) was stirred at 50 °C for 2 h. Then the mixture was concentrated. The residue was purified by flash chromatography (MeCN/H20 = 1/5 to 3/2) to afford methyl 2-(((5-chloro-lH-indazol-3- yl)methyl)amino)-2-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate as a white solid (31 mg, 89%). LRMS (M+H+) m/z calculated 528.2, found 528.2. ¾ NMR (DMSO-Λ, 300 MHz) δ 12.98 (s, 1H), 8.36 (m, 2H), 7.89 (s, 1H), 7.75-7.72 (m, 1H), 7.61 (s, 1H), 7.52-7.49 (m, 1H), 7.42-7.30 (m, 2H), 7.20- 7.16 (m, 4H), 6.40-6.37 (m, 1H), 6.22-6.18 (m, 1H), 5.04 (d, 2H), 4.49-4.45 (m, 1H), 3.99-3.91 (m, 4H), 3.56 (s, 3H), 3.28-3.23 (m, 1H).
Example 29: Preparation of 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)pyridin-3-yl)acetic acid
Figure imgf000090_0001
Figure imgf000090_0002
To a solution of methyl 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)pyridin-3-yl)acetate (23 mg, 0.037 mmol, 1.0 eq) in MeOH (3 mL) and H2O (2 mL) was added NaOH (15 mg, 0.38 mmol, 10.0 eq). The mixture was stirred at 70 °C for 2 h. The organic solvents were evaporated. To the residue was added cone. HCl till pH 3 and then evaporated under reduced pressure to give a solid, which was further purified by flash chromatography (MeCN/l hO = 1/9 to 3/2) to afford 2-(((5- chloro- 1 H-indazol-3 -yl)methyl)amino)-2-(5-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)pyridin-3 -yl)acetic acid as a white solid (19 mg, 85%). LRMS (M+H+) m/z calculated 514.1, found 514.1. ¾ NMR (DMSO-ifc, 300 MHz) δ 13.12 (s, 1H), 8.35 (d, 2H), 7.93 (s, 1H), 7.74 (d, 1H), 7.63 (d, 1H), 7.53 (q, 1H), 7.42-7.32 (m, 2H), 7.19-7.16 (m, 4H), 6.39 (m, 1H), 6.20 (t, 1H), 4.35 (s, 1H), 4.10 (d, 2H), 3.88 (s, 2H).
Example 30: Preparation of 2-(5-chloro-lH-indazole-3-carboxamido)-2-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)pyridin-3-yl)acetic acid
Figure imgf000090_0003
A mixture of methyl 2-amino-2-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate (202 mg, 0.46 mmol, 1.0 eq), 5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carboxylic acid (130 mg, 0.46 mmol, 1.0 eq), HATU (176 mg, 0.46 mmol, 1.0 eq) and DIEA (1 mL) in DCM (30 mL) was stirred at rt for 20 h. Then the mixture was diluted with DCM (30 mL), washed with saturated NaHCC>3 aqueous (20 mL) and then brine, dried over Na2S04, filtered and concentrated in vacuo to give an oil, which was further purified by flash chromatography (MeCN/H20 = 1/2 to 19/1) to afford methyl 2-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)- lH-indazole-3-carboxamido)-2-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate as a white solid (150 mg, 51%).
Figure imgf000091_0001
To a solution of methyl 2-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carboxamido)-2-(5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate (100 mg, 0.15 mmol, 1.0 eq) in MeOH (10 mL) and H2O (5 mL) was added NaOH (64 mg, 1.5 mmol, 10.0 eq). The mixture was then stirred at rt for 20 h. The organic solvents were evaporated. To the residue was added cone. HC1 till pH 3. The mixture was then evaporated under reduced pressure to give a solid, which was further purified by flash chromatography (MeCN/H20 = 1/2 to 19/1) to afford 2-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carboxamido)- 2-(5-(4-((2-oxopyri 81 mg, 83%).
Figure imgf000091_0002
A solution of 2-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carboxamido)-2-(5-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetic acid (40 mg, 0.065 mmol, 1.0 eq) in HC1/EA (3 N, 5 mL) was stirred at rt for 20 h. Then the mixture was concentrated. The residue was purified by flash chromatography (MeCN/H20 = 1/9 to 3/2) to afford 2-(5-chloro-lH-indazole-3-carboxamido)-2-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)pyridin-3-yl)acetic acid as a white solid (9 mg, 26%>). LRMS (M+H+) m/z calculated 528.1, found 528.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.13 (s, 1H), 7.91 (s, 1H), 7.74 (d, 1H), 7.53 (m, 1H), 7.41- 7.32 (m, 2H), 7.24-7.10 (m, 8H), 7.08 (d, 1H), 6.38 (d, 1H), 6.20 (t, 1H), 5.02 (s, 2H), 4.23 (s, 1H), 4.04 (d, 2H), 3.87 (s, 2H).
Example 31: Preparation of l-(4-((4-(l-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2,2,2-trifluoroethyl)-lH- pyrazol- 1 -yl)methyl)benzyl)pyridin-2( 1 H)-one
Figure imgf000092_0001
1 ,4 ,,4 (1 ((/5 chioro 1 H indazo| 3 yKmethykaminoj 2'2'2 tnfluoroethyK 1 H pyrazo| 1
yl)methy|)benzy|)pyric|in"2(1 H)"one
Figure imgf000092_0002
4-iodo-lH-pyrazole (5.03 g, 25.92 mmol, 1.0 eq) was dissolved in dry THF (40 mL), NaH (4.16 g, 60%, 4.0 eq) was added in portions. T he resulting mixture was stirred at rt for 30 min. To this mixture was added (2- (chloromethoxy)ethyl)trimethylsilane (6.47 g, 38.89 mmol, 1.5 eq) dropwise. The reaction was stirred at ambient temperature for 2 h, then poured into saturated aqueous NH4CI. The mixture was extracted with EA, washed with water, brine, dried over anhydrous sodium sulfate, and purified on silica gel column to afford desired 4-iodo-l -((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrazole (7.5 g, 89%).
Figure imgf000092_0003
To the solution of triethylamine (14.6 mL, 105 mmol, 1.0 eq), piperidine (12.6 g, 126 mmol, 1.2 eq) in DCM (40 mL) was added trifluoroacetic anhydride (14.8 mL, 105 mmol, 1.0 eq) at 0 °C over 30 min. The resulted mixture was stirred at 0 °C for 30 min then at rt for 1 h, and then quenched with water. The DCM layer was washed with IN HCl, brine, dried over anhydrous sodium sulfate, filtered and evaporated to dryness to afford 2,2,2-trifluoro-l-(piperidin-l-yl)ethanone as yellow oil (13.2 g, 70%).
Figure imgf000092_0004
To a solution of 4-iodo-l -((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrazole (4.0 g, 12.34 mmol, 1.0 eq) in dry THF (100 mL) at 0 °C was added iPrMgBr (1.0 M, 18.5 mL, 18.5 mmol, 1.5 eq) quickly. The mixture was stirred for 30 min, then cooled to -78 °C. a solution of 2,2,2-trifluoro-l-(piperidin-l-yl)ethanone (4.46 g, 24.68 mmol, 2.0 eq, dissolved in 20 mL of THF) was added quickly. The reaction mixture was stirred for 1 h, quenched with saturated aqueous NH4CI, extracted with EA. The extracts were washed with water, brine, dried over Na2S04, filtered and purified on silica gel column (PE/EA = 10/1) to afford 2,2,2-trifluoro-l-(l-((2- (trimethylsilyl)ethoxy)methyl)-lH-pyrazol-4-yl)ethanone as colorless oil (5.2 g, crude).
Figure imgf000092_0005
2,2,2-trifluoro-l-(l -((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrazol-4-yl)ethanone (5.23 g, crude, obtained above) was dissolved in pyridine (45 mL) and EtOH (15 mL). H2NOH.HCl (1.35 g, 19.46 mmol) was added to this solution. The reaction mixture was stirred at 60 °C for 3 h, and then concentrated. The residue wasdissolved in EA (100 mL), washed with 1 N HC1 (100 m L X2), brine, dried over anhydrous sodium sulfate, and purified via flash chromatography to afford 2,2,2-trifluoro-l-(l -((2- (trimethylsilyl)
Figure imgf000093_0001
2,2,2-trifluoro-l-(l -((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrazol-4-yl)ethanone oxime (3.4 g, 11.0 mmol, 1.0 eq) and Raney Ni were mixed in MeOH (30 mL) and hydrogenated overnight, filtered through celite, concentrated, purified via flash chromatography to afford 2,2,2-trifluoro-l -(l-((2- (trimethylsilyl)ethoxy)methyl)-lH-pyrazol-4-yl)ethanamine (1.2 g, 80% purity) which was used without further purificatio
Figure imgf000093_0002
The solution of 2,2,2-trifluoro-l-(l -((2-(trimethylsilyl)ethoxy)methyl)-lH-pyrazol-4-yl)ethanamine (1.2 g, 4.0 mmol, 1.0 eq) in methanol (3 mL) was treated with TFA (1 mL) and stirred at 30 °C for 30 min. The mixture was concentrated, basified with saturated aqueous NaHC03, and concentrated. The residue was purified via flash chromatography to afford 2,2,2-trifluoro-l-(lH-pyrazol-4-yl)ethanamine as a white solid (400 mg, 59%).
Figure imgf000093_0003
To a solution of 2,2,2-trifluoro-l -(lH-pyrazol-4-yl)ethanamine (200 mg, 1.2 mmol, 1.0 eq) in dry THF (10 mL) was added NaHMDS (2.0 M, 0.575 mL, 1.15 mmol, 0.95 eq) at -10 °C. The mixture was stirred at this temperature for 30 min, and then l-(4-(chloromethyl)benzyl)pyridin-2(lH)-one (282 mg, 1.21 mmol, 1.0 eq) was added. Tthe cool bath was removed and the reaction mixture was stirred at 60 °C overnight, quenched with saturated aqueous NH4CI, concentrated. The residue was purified via flash chromatography to afford 1 - (4-((4-(l-amino-2,2,2 rifluoroethyl)-lH-pyrazol-l -yl)methyl)benzyl)pyridin-2(lH)-one as a white solid (230 mg, 52%
Figure imgf000093_0004
To the mixture of l-(4-((4-(l-amino-2,2,2-trifluoroethyl)-lH-pyrazol-l-yl)methyl)benzyl)pyridin-2(lH)- (110 mg, 0.317 mmol, 1.0 eq) in MeOH (2 mL) was added 5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazole-3-carbaldehyde (84 mg, 0.317 mmol, 1.0 eq) followed by two drops of HOAc.The mixture was stirred for 1 h, and NaBH4 (48 mg, 1.26 mmol, 4.0 eq) was added in portions till the reaction was complete. The mixture was concentrated, and the residue was dissolved in DCM, washed with water, brine, dried over anhydrous sodium sulfate, and purified via flash chromatography to afford l-(4-((4-(l -(((5-chloro-l - (tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)amino)-2,2,2-trifluoroethyl)-lH-pyrazol-l - yl)methyl)be
Figure imgf000094_0001
l-(4-((4-(l -(((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)amino)-2,2,2 ri
lH-pyrazol-l -yl)methyl)benzyl)pyridin-2(lH)-one (110 mg, 0.18 mmol, 1.0 eq) was stirred in TFA (1.5 mL) for 1 h, and then concentrated. The residue was neutralized with 1 N NaOH, concentrated, and triturated in 20% MeOH in DCM. The filtrate was concentrated and purified via reverse flash chromatography to afford 1 - (4-((4-(l-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2,2,2-trifluoroethyl)-lH-pyrazol-l- yl)methyl)benzyl)pyridin-2(lH)-one as a white solid (45 mg, 52%). LRMS (M+H)+ m/z calculated 527.1, found 527.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 12.96 (s, 1 H), 7.90 (s, 1 H), 7.85 (s, 1 H), 7.77 (d, 1 H), 7.54- 7.46 (m, 2 H), 7.41 (dt, 1 H), 7.32 (d, 1 H), 7.25 (d, 2 H), 7.16 (d, 2 H), 6.40 (d, 1 H), 6.22 (t, 1 H), 5.28 (s, 2 H), 5.06 (s, 2 H), 4.39 - 4.28 (m, 1 H), 4.08 (dd, 1 H), 3.98 (dd, 1 H), 3.18-3.08 (m, 1 H).
Example 32: Preparation of 5-chloro-N-(2,2,2-trifluoro-l-(l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH- pyrazol-4-yl)ethyl)-lH-indazole-3-carboxamide
Figure imgf000094_0002
5"c |oro"A/"(2'2'2"tnf|uoro"r(r(4 " ((2"oxopyridin"i (2H)"
yljmet yljbenzyi^H^yrazoi^'yi^ ylj lH'indazoie'a'c^r dxamicie
Figure imgf000094_0003
The mixture of 5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carboxylic acid (124 mg, 0.44 mmol, 1.0 eq ), HATU (251 mg, 0.66 mmol, 1.5 eq) and DIEA (0.2 mL) in DCM (10 mL) was stirred for 10 min and then l-(4-((4-(l-amino-2,2,2-trifluoroethyl)-lH-pyrazol-l-yl)methyl)benzyl)pyridin-2(lH)-one (160 mg, 0.44 mmol, 1.0 eq) was added. The reaction mixture was stirred for 1.5 h, quenched with saturated aqueous sodium bicarbonate. The organic layer was washed with water, brine, and purified via flash chromatography to afford 5-chloro-l-(tetrahydro-2H-pyran-2-yl)^
lH-pyrazol
Figure imgf000095_0001
The mixture of 5-chloro-l -(tetrahydro-2H-pyran-2-yl)-N-(2,2,2-trifluoro-l-(l-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-lH-pyrazol-4-yl)ethyl)-lH-indazole-3-carboxamide (200 mg, 0.32 mmol, 1.0 eq) in trifluoroacetic acid was stirred for 1 h, then concentrated and added with saturated aqueous sodium carbonate and DCM. After extraction, the organic phase was concentrated and purified via reverse flash chromatography to afford 5-chloro-N-(2,2,2 rifluoro-l-(l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazol-4-yl)ethyl)- lH-indazole-3-carboxamide as a white solid (80 mg, 46%). LRMS (M+H)+ m/z calculated 460.1, found 460.1. ¾ NMR (DMSO-d6, 400 MHz) δ (ppm) 13.96 (s, 1 H), 9.43 (d, 1 H), 8.10 (s, 2 H), 7.75 (dd, 2 H), 7.70 (d, 1 H), 7.47 (dd, 1 H), 7.40 (dt, 1 H), 7.23 (m, 4 H), 6.39 (d, 1 H), 6.20 (dt, 1 H), 6.06 (t, 1 H), 5.30 (s, 2 H), 5.05 (s, 2 H). Example 33: Preparation of l-(4-(3-(l-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2,2,2- trifluoro
Figure imgf000095_0002
To a solution of l-(3-bromophenyl)-2,2,2-trifluoroethanone (1.01 g, 4.0 mmol, 1 eq) in toluene (20 mL) was added LiHMDS (1 M in THF, 4.4 mL, 1.1 eq) dropwise at rt and the mixture was stirred at rt for 15 min. Then B¾-Me2S (2 M, 4 mL, 2.0 eq) was added. The mixture was stirred at rt for 20 min. The mixture was diluted with water, extracted with EA (3x20 mL). The combined organic layers were concentrated, and purified by flash chromatography (PE/EA = 10/1-5/1) to afford l-(3-bromophenyl)-2,2,2-trifluoroethanamine as a white solid (500 mg, 50%).
Figure imgf000096_0001
To a solution of l -(3-bromophenyl)-2,2,2-trifluoroethanamine (506 mg, 2.0 mmol, 1 eq), and TEA (606 mg, 6.0 mmol, 3 eq) in DCM (10 mL) was added TFAA (462 mg, 2.2 mmol) in 3 mL of DCM at 0 °C under N2. The reaction mixture was stirred at rt for 2 h, diluted with water, and extracted with EA (3x20 mL). The combined organic layers were washed with brine, concentrated, and purified on silica gel column (PE/EA = 20/1-10/1) to afford N-(l -(3-bromophenyl)-2,2,2-trifluoroethyl)-2,2,2-trifluoroacetamide as a colorless oil (600 mg, 86%).
Figure imgf000096_0002
A mixture ofN-(l-(3-bromophenyl)-2,2,2-trifluoroethyl)-2,2,2-trifluoroacetamide (140 mg, 0.47 mmol, 1.0 eq), (Pin)2B2 (140 mg, 0.47 mmol, 1.0 eq), Pd(dppf)Cl2 (140 mg, 0.47 mmol, 1.0 eq) and AcOK (140 mg, 0.47 mmol, 1.0 eq) in dioxane (8 mL) was stirred at 80 °C under N2 atmosphere overnight. The mixture was diluted with water, extracted with EA (3x20 mL). The combined organic layers were washed with brine, and concentrated. The residue was purified by prep-TLC to afford 2,2,2-trifluoro-N-(2,2,2-trifluoro-l -(3-(4,4,5,5- tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)ethyl)acetamide as a white solid (14 mg, 10%).
Figure imgf000096_0003
A mixture of 2,2,2-trifluoro-N-(2,2,2-trifluoro-l-(3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)phenyl)ethyl)acetamide (200 mg, 0.50 mmol, 1.0 eq) and l-(4-(bromomethyl)benzyl)pyridin-2(lH)-one (140 mg, 0.50 mmol, 1.0 eq), Pd(PPh3)4 (58 mg, 0.050 mmol, 0.1 eq) and Na2C03 (159 mg, 1.50 mmol, 3.0 eq) in dioxane-H20 (7 mL / 1 mL) was stirred at 80 °C under N2 atmosphere overnight. Then the mixture was filtered and the filtrate concentrated. The residue was diluted with water, extracted with EA (3x20 mL). The combined organic layers were washed with brine, concentrated. The residue was purified by prep-TLC to afford 2,2,2-trifluoro-N-(2,2,2-trifluoro-l -(3-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)phenyl)ethyl)acetamide as a white solid (70 mg, 30%>).
Figure imgf000097_0001
To a solution of 2,2,2-trifluoro-N-(2,2,2-trifluoro-l -(3-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)phenyl)ethyl)acetamide (70 mg, 0.15 mmol, 1.0 eq) in ΜΘΟΗ/]¾0 (10 mL / 2 mL) was added KOH (252 mg, 0.48 mmol, 30 eq). The mixture was refluxed for 2 h. It was diluted with water, extracted with EA (3x20 mL). The combined organic layers were washed with brine, concentrated, purified by prep-TLC (PE/EA = 1/1) to afford l-(4-(3-(l-amino-2,2,2-trifluoroethyl)benzyl)benzyl)pyridin-2(lH)-one (40 mg, 72%).
Figure imgf000097_0002
To a solution of l-(4-(3-(l-amino-2,2,2-trifluoroethyl)benzyl)benzyl)pyridin-2(lH)-one (40 mg, 0.11 mmol, 1 eq) in MeOH (10 mL) was added 5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carbaldehyde (29 mg, 0.11 mmol, 1.0 eq), followed with AcOH (13.2 mg, 0.22 mmol, 2 eq). The mixture was stirred at rt for 1 h. NaBH4 (100 eq) was added in 3 portions. The mixture was heated at 50 °C overnight, then diluted with water, extracted with EA (3x20 mL). The combined organic layers were washed with brine, concentrated, purified by prep-TLC (PE/EA = 1/1) to afford l-(4-(3-(l-(((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)amino)-2,2,2-trifluoroethyl)benzyl)benzyl)pyridin-2(lH)-one as a brown solid (30 mg, 44%).
Figure imgf000097_0003
To a stirred suspension of l-(4-(3-(l-(((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)amino)-2,2,2-trifluoroethyl)benzyl)benzyl)pyridin-2(lH)-one (20 mg, 0.032 mmol, 1.0 eq) in EA (3 mL) was added EA/HCl (4 N, 1 mL). The mixture was stirred at rt for 4 h. After concentration, it was purified by prep-HPLC to afford l-(4-(3-(l-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2,2,2- trifluoroethyl)benzyl)benzyl)pyridin-2(lH)-one HC1 salt as a white solid (10.0 mg, 51%>). LRMS (M+H+) m/z caculated 537.16, found 537.2. ¾ NMR (CD3OD, 400 MHz) δ 7.91 (d, 1 H), 7.73 (t, 1 H), 7.58 (m, 1 H), 7.48-7.35 (m, 5 H), 7.29-7.24 (dd, 4 H),6.77 (d, 1 H), 6.66(t, 1 H), 5.50 (m, 1 H), 5.26 (s, 2 H), 4.50 (q, 2 H) 3.99 (s, 2 H).
Example 34: Preparation of methyl 2-(5-chloro-lH-indazol-3-yl)-2-(3-(4-((2-oxopyridin-l
yl)methyl)be
Figure imgf000098_0001
methyl
Figure imgf000098_0002
To the solution of NaOH (440 mg, 11 mmol, 1.1 eq.) in H2O (10 mL) was added 5-chloroindoline-2,3-dione (1.81 g, 10 mmol, 1.0 eq). The dark solution was cooled to 0-5 °C. A solution of NaNC (828 mg, 12 mmol, 1.2 eq) in H2O (3 mL) was added. The mixture was added to a solution of cone. H2SO4 (2.90 g, 30 mmol, 3.0 eq) in H20 (30 mL) at 0-5 °C. The mixture was stirred at 0-5 °C for 30 min. A solution of SnCl2 H20 (5.64 g, 25 mmol, 2.5 eq) in cone. HC1 (15 mL) was added and the yellow slurry was stirred at rt for 2.5 h. Filtered and the solid was collected to afford 5-chloro-lH-indazole-3-carboxylic acid as a yellow solid (2 g crude, quant).
Figure imgf000098_0003
To the solution of 5 -chioro- lH-indazole-3-carboxylic acid (2 g, 10 mmol, 1.0 eq.) in MeOH (100 mL) was added cone. H2SO4 (3 drops). The mixture was stirred at 75 °C overnight. Filtered and the filtrate was concentrated. The residue was partitioned between saturated NaHCCb aqueous solution (20 mL) and DCM (20 mL). The aqueous layer was extracted with DCM (20 mLX2). The combined organic extracts were dried over Na2S04 and concentrated to afford methyl 5-chloro-lH-indazole-3-carboxylate as a yellow solid (1.28 g, 61%).
Figure imgf000098_0004
The mixture of methyl 5-chloro-lH-indazole-3-carboxylate (1.28 g, 6.1 mmol, 1.0 eq), 3,4-dihydro-2H-pyran
(1.7 mL, 18.7 mmol, 3.0 eq), TsOH H20 (120 mg, 0.63 mmol, 0.1 eq) in THF (100 mL) as stirred at 70 °C overnight. The mixture was concentrated and the residue was purified on silica column to afford methyl 5- chloro-l-(tetrahydro-2H-pyra -2-yl)-lH-indazole-3-carboxylate as a yellow solid (1.27 g, 73.4 %).
Figure imgf000099_0001
To a solution of methyl 5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carboxylate (100 mg, 0.34 mmol, 1.0 eq) was added a solution of DIBAL-H in hexane (1 mol/L, 1 mL, 3.0 eq.) at -70 °C. The mixture was stirred at -78 °C for 1 h and warmed to rt for 2 h. The reaction was quenched with saturated NH4CI aqueous solution (10 mL) and extracted with DCM (10 mL X 2). The combined organic extracts were dried over Na2S04 and concentrated to afford (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanol as a white solid (91 mg, quant)
Figure imgf000099_0002
The mixture of (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanol (2.0 g, 7.5 mmol, 1.0 eq.), PCC (4.85 g, 22.5 mmol, 3.0 eq.), and silica gel (100-200 mesh, 3 g) in DCM (60 mL) was stirred at rt for 5 h. Filtered and the filtrate was concentrated. The residue was purified on silica gel column to afford 5-chloro-l- (tetrahydro-2H-pyran-2-yl)- -indazole-3-carbaldehyde as a light solid (1.18 g, 59.4%).
Figure imgf000099_0003
To a solution of 5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carbaldehyde (723 mg, 2.73 mmol, 1.0 eq.) in NFL/MeOH (15 mL) was added (z-PrO)4Ti (0.1 mL). The mixture was stirred at rt for 1 h. BF3 Et20 (3 drops) was added and followed by addition of TMSCN (1.1 mL, 8.81 mmol, 3.2 eq.). The mixture was stirred at rt overnight and quenched with H2O (50 mL). Extracted with DCM (50 mL X 3) and the combined organic extracts were dried over Na2S04 and concentrated. The residue was purified on silica gel column to afford 2- amino-2-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)acetonitrile as a yellow solid (704 mg,
88.4%).
Figure imgf000099_0004
A mixture of 2-amino-2-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)acetonitrile (83 mg, 0.285 mmol, 1 eq), (92 mg , 0.288 mmol, 1.0 eq), HATU (145 mg, 0.38 mmol, 1.3 eq), and DIPEA (0.8 mL) in DMF (3 mL) was stirred at 50 °C overnight. The mixture was purified via prep- MPLC to afford N-((5- chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)(cyano)methyl)-3-(4-((2-oxopyridin-l(2H)- yl)methyl)b
Figure imgf000100_0001
To a solution of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)(cyano)methyl)-3-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)benzamide (142 mg, 0.24 mmol, 1.0 eq) in DMSO (3 mL) was added K2CO3 (40 mg, 0.29 mmol, 1.2 eq) and H2O2 (30% aq., 0.5 mL). The mixture was stirred at rt overnight and purified via prep-MPLC to afford N-(2-amino-l -(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)-2- oxoethyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide as a white solid (75 mg, 51.3%).
Figure imgf000100_0002
A solution of N-(2-amino-l -(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)-2-oxoethyl)-3-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)benzamide (75 mg, 0.123 mmol, 1 eq) in MeOH (2 mL) and
HCl/dioxane (5 N, 2 mL) was stirred at rt overnight, then concentrated. The residue was purified via prep- TLC (DCM/MeOH = 15/1, v/v) to afford methyl 2-(5-chloro-lH-indazol-3-yl)-2-(3-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)benzamido)acetate as a white solid (15 mg, 22.6 %). LRMS (M+H+) m/z calculated 541.2, found 541.1. ¾ NMR (CD3OD, 400 MHz) δ 7.88 (s, 1 H), 7.76 (s, 1 H), 7.73-7.69 (m, 2 H), 7.57 (d, 1 H), 7.54-7.51 (m, 1 H), 7.42-7.39 (m, 3 H), 7.23 (dd, 4 H), 6.58 (d, 1 H) , 6.40 (t, 1 H), 6.20 (s, 1 H), 5.18 (s, 2 H), 4.03 (s, 2 H), 3.81 (s, 3 H).
Example 35: Preparation of methyl 2-(5-chloro-lH-indazol-3-yl)-2-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)acetate
Figure imgf000101_0001
A mixture of 2-amino-2-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)acetonitrile (290 mg, 1 mmol, 1 eq), 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (320 mg , 1 mmol, 1.0 eq), HATU (500 mg, 1.3 mmol, 1.3 eq), and DIPEA (1 mL) in DMF (5 mL) was stirred at 50 °C overnight. The mixture was mixed with water (20 mL) and filtered. The solid was collected and purified on silica gel column to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)(cyano)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)
Figure imgf000101_0002
To the solution of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)(cyano)methyl)-5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (237 mg, 0.4 mmol, 1.0 eq) in DMSO (5 mL) was added K2CO3 (28 mg, 0.2 mmol, 0.5 eq) and H2O2 (30% aq., 0.5 mL). The mixture was stirred at rt overnight and purified via prep-MPLC to afford N-(2-amino-l -(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)-2- oxoethyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (150 mg, 61.4%).
Figure imgf000101_0003
A solution of N-(2-amino-l -(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)-2-oxoethyl)-5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (110 mg, 0.18 mmol, 1 eq.) in HC1/ MeOH (5 N, 5 mL) was stirred at rt overnight, then concentrated. The residue was purified via prep-MPLC and further purified via prep-TLC (DCM/MeOH = 20/1, v/v) to afford methyl 2-(5-chloro-lH-indazol-3-yl)-2-(5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)acetate as a white solid (60 mg, 61 %). LRMS (M+H+) m/z calculated 542.2, found 542.2. ¾ NMR (CD30D, 300 MHz) δ 8.85 (d, 1 H), 8.57 (d, 1 H), 8.12 (s, 1 H), 7.85 (d, 1 H), 7.68 (dd, 1 H), 7.57-7.50 (m, 2 H), 7.40 (dd, 1 H), 7.25 (dd, 4 H), 6.57 (d, 1 H), 6.38 (t-d, 1 H), 6.20 (s, 1 H), 5.17 (s, 2 H), 4.07 (s, 2 H), 3.80 (s, 3 H).
Example 36: Preparation of N-(l-(5-chloro-lH-indazol-3-yl)-2-hydroxyethyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000102_0001
To the solution of methyl 2-(5-chloro-lH-indazol-3-yl)-2-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)acetate (82 mg, 0.15 mmol, 1.0 eq) and NaBH4 (42 mg, 1.1 mmol, 7 eq) in THF (4 mL) was added MeOH (0.3 mL) dropwise at 55 °C. The mixture was stirred at 60 °C for 1 h, cooled to rt and quenched with water (7 drops). Filtered and the filtrate was concentrated. The residue was purified via prep-HPLC to afford N-(l-(5-chloro-lH-indazol-3-yl)-2-hydroxyethyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a white solid (22 mg, 28.2%). LRMS (M+H+) m/z calculated 514.2, found 514.1. 'H NMR (CD3OD, 400 MHz) δ 8.83 (d, 1 H), 8.52 (s, 1 H), 8.22 (s, 1 H), 7.75 (d, 1 H), 7.56 (dd, 1 H), 7.43-7.37 (m, 2 H), 7.23 (dd, 1 H), 7.15 (dd, 4 H), 6.44 (d, 1 H), 6.26 (t, 1 H), 5.60 (t, 1 H), 5.05 (s, 2 H), 4.02 (d, 2 H), 4.01 (s, 2 H). Example 37: Preparation of N-(l-(5-chloro-lH-indazol-3-yl)ethyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000103_0001
Λ/"(1"(5"ch "1 HΊndazoΓ3"y|Jethy|)"5"(4"((2"oxopyridin"1(2H)"yl)methy|)benzy|Jnicotinamic|e
Figure imgf000103_0002
To the solution of 5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazole-3-carbaldehyde (260 mg, 0.98 mmol, 1.0 eq.) in THF (5 mL) was added a solution of CHsMgBr in THF (3 mol/L, 0.5 mL, 1.5 eq). The mixture was stirred at rt overnight. The mixture was quenched with water (10 mL) and extracted with EA (20 mL X 2). The combined organic layers were dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography (PE/EA = 10/1, v/v) to afford l-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol- 3-yl)ethanol as a white solid (270 mg, 97.9%).
I
Figure imgf000103_0003
To the solution of l-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)ethanol (280 mg, 1.0 mmol, 1.0 eq), isoindoline-l,3-dione (156 mg, 1.06 mmol, 1.06 eq), PPI13 (314 mg, 1.2 mmol, 1.2 eq) in THF (10 mL) was added DIAD (0.3 mL, 1.5 eq) at 0 °C. The mixture was stirred at rt overnight and partitioned between EA (20 mL) and water (10 mL). The aqueous layer was extracted with EA (20 mL X 2). The combined organic layers were dried over anhydrous Na2S04 and concentrated. The residue was purified via prep-TLC (PE/EA = 7/1, v/v) to afford 2-(l -(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)ethyl)isoindoline-l,3-dione as a white solid (150 mg, 36.6%).
Figure imgf000103_0004
To a solution of 2-(l-(5-c oro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)ethyl)isoindoline-l,3-dione (70 mg, 0.18 mmol, 1.0 eq.) in MeOH (4 mL) was added ΝΗ2ΝΗ2Ή20 (0.10 mL). The mixture was stirred at rt for 3 h. The volatiles were concentrated and to the residue was added DCM (10 mL) and stirred at rt for 1 h, then filtered through Celite. The filtrate was concentrated to afford l-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)- lH-indazol-3-yl)ethanamine as a white solid (54 mg crude, quant).
Figure imgf000104_0001
A mixture of 5-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)nicotinic acid (58 mg, 0.18 mmol, 1 eq), l-(5- chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)ethanamine (54 mg , 0.18 mmol, 1.0 eq), HATU (69 mg, 0.18 mmol, 1.0 eq), and DIPEA (0.3 mL) in DMF (5 mL) was stirred at rt overnight. The mixture was purified via prep- MPLC to afford N-(l-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)ethyl)-5-(4- ((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (58 mg, 61%).
Figure imgf000104_0002
To a solution of N-(l -(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)ethyl)-5-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)nicotinamide (58 mg, 0.1 mmol, 1 eq) in MeOH (5 mL) was added HC1/EA (5 N, 3 mL) and the mixture was stirred at rt overnight. The volatiles were concentrated and to the residue was added NFL/MeOH (3 N, 5 mL) and concentrated again. The rresidue was purified via prep-TLC (MeOH/DCM = 1/15, v/v) to afford N-(l -(5-chloro-lH-indazol-3-yl)ethyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a yellow solid (35 mg, 71.4%). LRMS (M+H+) m/z calculated 498.2, found 498.2. 'H NMR (DMSO-ifc, 300 MHz) δ 13.09 (s, 1 H), 9.14 (d, 1 H), 8.86 (s, 1 H), 8.61 (s, 1 H), 8.05 (s, 1 H), 7.86 (s, 1 H), 7.74 (d, 1 H), 7.53 (d, 1 H), 7.40 (t, 1 H), 7.32 (d, 1 H), 7.25 -7.18 (m, 4 H), 6.38 (d, 1 H), 6.20 (t, 1 H), 5.62 (t, 1 H), 5.03 (s, 2 H), 3.99 (s, 2 H), 1.65 (d, 3 H).
Example 38: Preparation of N-(l-(5-chloro-lH-indazol-3-yl)ethyl)-5-((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)nicotinamide
Figure imgf000104_0003
N (1 (5 chioro 1H indazo| 3 yl^ethyl) 5 ((6 ((2 oxopyrjdin 1 (2H) yljmethylj yndin 3 yl/nethyljnicotinamjde
Figure imgf000105_0001
To a solution of 5-((6-((2-oxopyridin-l (2H)-yl)methyl)pyridin-3-yl)methyl)nicotinic acid (500 mg crude, 0.5 mmol, 1.0 eq) in DMF (5 mL) were added DIPEA (194 mg, 1.5 mmol, 3.0 eq) and HATU (228 mg, 0.6 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, l-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)- lH-indazol-3-yl)ethanamine (170 mg crude, 0.5 mmol, 1.0 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-(l-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)ethyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide as a yellow solid (130 mg, 45%).
Figure imgf000105_0002
To a solution of N-(l -(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)ethyl)-5-((6-((2-oxopyridin- l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide (130 mg, 0.22 mmol, 1.0 eq) in MeOH (5 mL) was added HC1/EA (5 mL) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL), neutralized with saturated NaHC03 (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-(l - (5-chloro-lH-indazol-3-yl)ethyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide as a white solid (70 mg, 64%). LRMS (M+H+) m/z caculated 499.1, found 499.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.03 (brs, 1 H), 9.12 (d, 1 H), 8.87 (d, 1 H), 8.65 (d, 1 H), 8.47 (d, 1 H), 8.06 (t, 1 H), 7.86 (d, 1 H), 7.75 (dd, 1 H), 7.64 (dd, 1 H), 7.53 (d, 1 H), 7.48-7.40 (m, 1 H), 7.33 (dd, 1 H), 7.13 (d, 1 H), 6.37 (d, 1 H), 6.23 (td, 1 H), 5.63 (t, 1 H), 5.12 (s, 2 H), 4.03 (s, 2 H), 1.66 (d, 3 H).
Example 39: Preparation of methyl 3-(lH-indazol-3-yl)-3-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)propanoate
Figure imgf000106_0001
methyl 3 (1 H indazoi 3 y|j 3 ^5 ^ ^2 oxopyridin ΐ βΗ) yljmethyljbenzyijnicotinamidOjpropanoate
Figure imgf000106_0002
Methyl 3-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)acrylate (200 mg, 0.62 mmol, 1.0 eq) and benzylamine (1 mL) were combined and then heated to 70 °C under N2 overnight. After cooled to rt, the residue was purified by flash chromatography (MeCN/l hO = 1/2 to 19/1) to afford methyl 3-(benzylamino)-3- (5-chloro-l -(tetrahydro- -pyran-2-yl)-lH-indazol-3-yl)propanoate as an oil (188 mg, 70%).
Figure imgf000106_0003
A mixture of methyl 3-(benzylamino)-3-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)propanoate (140 mg, 0.32 mmol, 1.0 eq), 20% Pd(OH)2/C (50 mg) in methanol (20 mL) was stirred at rt under a hydrogen atmosphere (1 atm, balloon) for 1.5 h. The reaction mixture was filtered and the filtrate was concentrated to afford methyl 3-amino-3-(l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)propanoate as a yellow oil (90 mg, 90%).
Figure imgf000106_0004
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (95 mg, 0.29 mmol, 1.0 eq), methyl 3-amino-3-(l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)propanoate (90 mg, 0.29 mmol, 1.0 eq), HATU (113 mg, 0.29 mmol, 1.0 eq) and DIEA (1 mL) in DCM (20 mL) was stirred at rt for 3 h. The mixture was diluted with DCM (50 mL), washed with saturated NaHCC (25 mL), and the brine, dried over Na2SO i, filtered and concentrated in vacuo. The residue was then further purified by flash chromatography
(MeCN/H20 = 1/4 to 4/1) to afford methyl 3-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)-3- (l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)propanoate as a white solid (129 mg, 72%>).
Figure imgf000107_0001
A solution of methyl 3-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)-3-(l-(tetrahydro-2H- pyran-2-yl)-lH-indazol-3-yl)propanoate (125 mg, 0.20 mmol, 1.0 eq) in MeOH (3 mL) and HC1/EA (3 N, 5 mL) was stirred at 50 °C for 2 h. Then the mixture was concentrated. The residue was purified by flash chromatography (MeCN/H20 = 1/2 to 19/1) to afford methyl 3-(lH-indazol-3-yl)-3-(5-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)nicotinamido)propanoate as a white solid (82 mg, 76%). LRMS (M+H+) m/z calculated 522.2, found 522.2. ¾ NMR (DMSO-ifc, 300 MHz) δ 12.90 (s, 1H), 9.217 (d, 1H), 8.84 (s, 1H), 8.61 (s, 1H), 8.02 (s, 1H), 7.76-7.71 (m, 2H), 7.49 (d, 1H), 7.42-7.29 (m, 2H) , 7.21 (m, 4H), 7.07-7.02 (m, 1H), 6.38 (d, 1H), 6.23-6.18 (m, 1H), 6.02-5.99 (m, 1H), 5.03 (s, 2H), 3.97 (s, 2H), 3.55 (s, 3H), 3.36-3.28 (m, 1H), 3.12-3.04 (m, 1H).
Example 40: Preparation of methyl 3-(5-chloro-lH-indazol-3-yl)-3-(5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)propanoate
Figure imgf000107_0002
methyl S^chloro'iH^dazofs'yi^s"^"^^
Figure imgf000107_0003
A mixture of methyl 3-(benzylamino)-3-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)propanoate (140 mg, 0.32 mmol, 1.0 eq), 20% Pd(OH)2/C (50 mg), cone HC1 (0.5 mL) in methanol (25 mL) was stirred at rt under a hydrogen atmosphere (1 atm, balloon) for 1 h. The reaction mixture was filtered and the filtrate was concentrated to afford methyl 3-amino-3-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)propanoate as a yellow oil (76 mg, 64%>).
Figure imgf000108_0001
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (70 mg, 0.21 mmol, 1.0 eq), methyl 3-amino-3-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)propanoate (73 mg, 0.21 mmol, 1.0 eq), HATU (83 mg, 0.21 mmol, 1.0 eq) and DIEA (0.5 mL) in DCM (20 mL) was stirred at rt for 2 h.The mixture was diluted with DCM (30 mL), washed with saturated NaHCCb (20 mL), and the brine, dried over Na2SC>4, filtered and concentrated in vacuo. The residue was then further purified by flash chromatography
(MeCN/H20 = 1/2 to 19/1) to afford methyl 3-(5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)-3-(5- (4-((2-oxopyridi -l(2H)-yl)methyl)benzyl)nicotinamido)propanoate as a white solid (25 mg, 17%).
Figure imgf000108_0002
A solution of methyl 3-(5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)-3-(5-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)nicotinamido)propanoate (25 mg, 0.039 mmol, 1.0 eq) in MeOH (2 mL) and
HCl/MeOH (2 N, 3 mL) was stirred at rt overnight. Then the mixture was concentrated. The residue was purified by flash chromatography (MeCN/l hO = 1/19 to 3/2) to afford methyl 3-(5-chloro-lH-indazol-3-yl)- 3-(5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)propanoate as a white solid (16 mg, 73%). LRMS (M+H+) m/z calculated 556.2, found 556.2. ¾ NMR (DMSO-i/6, 300 MHz) δ 13.11 (s, 1H), 9.18 (d, 1H), 8.81 (d, 1H), 8.62 (d, 1H), 8.02 (s, 1H), 7.82 (s, 1H), 7.73 (dd, 1H), 7.53 (d, 1H), 7.41-7.31 (m, 2H) , 7.23-7.19 (m, 4H), 6.38 (d, 1H), 6.21-6.18 (m, 1H), 5.96-5.91 (m, 1H), 5.03 (s, 2H), 3.97 (s, 2H), 3.55 (s, 3H), 3.31-3.27 (m, 1H), 3.15-3.09 (m, 1H).
Example 41: Preparation of 2-(5-chloro-3-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol-l-yl)acetic acid 2~(5~ acid
Figure imgf000109_0001
The mixture of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide HC1 salt (260 mg, 0.5 mmol, 1.0 eq), methyl 2-chloroacetate (543 mg, 2.5 mmol, 5.0 eq) and CS2CO3 (1.0 g, 3 mmol, 6 eq) in DMF (5 mL) was was stirred at rt overnight. The mixture was poured into water and the precipitate was collected by suction to afford methyl 2-(5-chloro-3-((5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol-l -yl)acetate as a yellow solid (200 mg, 72.2%).
Figure imgf000109_0002
To the solution of methyl 2-(5-chloro-3-((5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)methyl)- lH-indazol-l-yl)acetate (200 mg, 0.36 mmol, 1 eq) in THF/MeOH (5 niL/10 mL) was added NaOH aqueous solution (1 N, 5 mL) and the mixture was stirred at rt overnight, then concentrated. The residue was acidified with HC1 to pH 3. The mixture was concentrated and the residue was purified via prep-MPLC to afford 2-(5- chloro-3-((5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol-l -yl)acetic acid as a yellow solid (140 mg, 74.3 %). LRMS (M+H+) m/z calculated 542.2, found 542.1. ¾ NMR (DMSO-d6, 300 MHz) δ 13.14 (s, 1H), 9.34 (t, 1H), 8.86 (s, 1 H), 8.62 (s, 1 H), 8.05 (s, 1 H), 7.93 (s, 1 H), 7.75 (d, 1 H), 7.67 (d, 1 H), 7.42-7.37 (m, 2 H), 7.25-7.19 (m, 4 H), 6.38 (d, 1 H), 6.21 (t, 1 H), 5.24 (s, 2 H), 5.03 (s, 2 H), 4.76 (d, 2 H), 3.99 (s, 2 H). Example 42: Preparation of N-((l-(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2- oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinamide
Figure imgf000110_0001
A mixture of 2-(5-chloro-3-((5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol- l-yl)acetic acid (110 mg, 0.20 mmol, 1 eq), NH3/MeOH (3 mol/L, 0.5 mL), NH4C1 (20 mg, 0.37 mmol, 1.9 eq), HATU (80 mg, 0.21 mmol, 1.05 eq), DIPEA (0.3 mL) in DMF (3 mL) was stirred at rt overnight. The mixture was purified via flash chromatography to afford N-((l -(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3- yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (41 mg, 37.3%).. LRMS (M+H+) m/z calculated 542.0, found 542.1. ¾ NMR (DMSO-d6, 300 MHz) δ 9.33 (t, 1H), 8.86 (s, 1 H), 8.61 (s, 1 H), 8.06 (s, 1 H), 7.86 (s, 1 H), 7.74 (d, 1 H), 7.45-7.37 (m, 3 H), 7.31-7.19 (m, 6 H), 6.39 (d, 1 H), 6.21 (t, 1 H), 5.04 (s, 2 H), 4.74 (s, 4 H), 3.98 (s, 2 H).
Example 43: Preparation of methyl 2-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)- 1 H-in
Figure imgf000110_0002
methyl 2"(5"chloro"3"((5"((6"((2"oxopyrjciin"i (2H)"yl)methyl)pyridin yljmethyljnjcotinamjdo Tiethyl)! H'iridazof 1 " yl^acetate
Figure imgf000111_0001
To a solution of N-((5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamide (200 mg crude, 0.41 mmol, 1.0 eq) in DMF (4 mL) were added methyl 2- chloroacetate (445 g, 4.1 mmol, 10.0 eq) and CS2CO3 (668 mg, 2.05 mmol, 5.0 eq). The mixture was stirred at rt overnight. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford methyl 2-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)nicotinamido)methyl)-lH-indazol-l-yl)acetate as a yellow solid (80 mg, 35%). LRMS (M+H+) m/z caculated 557.1, found 557.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.34 (t, 1 H), 8.88 (d, 1 H), 8.66 (d, 1 H), 8.47 (d, 1 H), 8.08 (d, 1 H), 7.94 (d, 1 H), 7.74 (dd, 1 H), 7.67 (d, 1 H), 7.64 (dd, 1 H), 7.48- 7.40 (m, 2 H), 7.13 (d, 1 H), 6.37 (d, 1 H), 6.23 (td, 1 H), 5.38 (s, 2 H), 5.12 (s, 2 H), 4.76 (d, 2 H), 4.03 (s, 2 H), 3.76 (s, 3 H).
Example 44: Preparation of 2-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)-lH-indazol-l -yl)acetic acid
Figure imgf000111_0002
The solution of methyl 2-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)-lH-indazol-l-yl)acetate (80 mg, 0.14 mmol, 1.0 eq) in MeOH (2 mL) and THF (2 mL) was treated with an aqueous solution of NaOH (56 mg, 1.4 mmol, 10 eq) in 2 mL of water. The mixture was stirred at rt overnight. Then the mixture was concentrated and the residue was acidified with 1 N HC1 till pH was 3. The mixture was purified by flash chromatography to give 2-(5-chloro-3-((5-((6-((2- oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamido)methyl)-lH-indazol-l -yl)acetic acid as a yellow solid (29 mg, 38%). LRMS (M+H+) m/z calculated 543.15, found 543.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.34 (t, 1H), 8.88 (s, 1H), 8.65 (s, 1H), 8.47 (s, 1H), 8.08 (s, 1H), 7.92 (s, 1H), 7.74 (d, 1H), 7.64 (d, 2H), 7.45-7.39 (m, 2H), 7.13 (d, 1H), 6.37 (d, 1H), 6.23 (t, 1H), 5.18 (s, 2H), 5.12 (s, 2H), 4.76 (d, 2H), 4.03 (s, 2H).
Example 45: Preparation of N-((l -(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2- oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide
Figure imgf000112_0001
A mixture of 2-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)-lH-indazol-l -yl)acetic acid (22 mg, 0.04 mmol, 1.0 eq), NH4CI (3.3 mg, 0.06 mmol, 1.5 eq), HATU (30.4 mg, 0.08 mmol, 2.0 eq) and DIEA (15 mg, 0.12 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt for 2 h. The mixture was poured into water (20 mL) and extracted with EtOAc (20 mL x2). The combined organic layer was washed with water, brine and dried over anhydrous sodium sulfate. The mixture was filtered and concentrated. The obtained residue was purified by flash columm to afford N-((l-(2- amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamide as a yellow solid (7 mg, 32%). LRMS (M+H+) m/z calculated 542.17, found 542.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.32 (t, 1H), 8.76 (s, 1H), 8.65 (s, 1H), 8.47 (s, 1H), 8.08 (s, 1H), 7.91 (s, 1H), 7.74 (d, 1H), 7.65-7.60 (m, 3H), 7.43-7.38 (m, 2H), 7.30 (s, 1H), 7.13 (d, 1H), 6.37 (d, 1H), 6.23 (t, 1H), 5.12 (s, 2H), 5.03 (s, 2H), 4.76 (d, 2H), 4.03 (s, 2H).
Example 46: Preparation of N-((5-chloro-l -(2-hydroxyethyl)-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin- 1 (2H)-yl)methyl)pyridin-3 -yl)methyl)nicotinamide
Figure imgf000113_0001
N~((5~chloro" 2 ydroxyethy H^n^
yl)methyl)nicotinar ilc|e
Figure imgf000113_0002
To a solution of methyl 2-(5-chloro-3-((5-((6-((2-oxopyridin-l (2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)-l H-indazol-l -yl)acetate (80 mg, 0.14 mmol, 1.0 eq) in THF (10 mL) was added NaBH4 (33 mg, 0.86 mmol, 6.0 eq). The mixture was heated at 60 °C. The mixture was added dropwise MeOH (2 mL) over 1 h and then heated at 60 °C for 1 h. The mixture was added water (5 mL). The mixture was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford 20 mg product, which was further purified via prep-HPLC to afford N-((5-chloro-l -(2-hydroxyethyl)- lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l (2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide as a yellow solid (7 mg, 9%). LRMS (M+H+) m/z caculated 529.1, found 529.2. ¾ NMR (CD3OD, 400 MHz) δ 8.75 (d, 1 H), 8.50 (s, 1 H), 8.33 (s, 1 H), 7.98 (s, 1 H), 7.74 (d, 1 H), 7.64 (dd, 1 H), 7.55 (dd, 1 H), 7.50-7.40 (m, 2 H), 7.25 (dd, 1 H), 7.14 (d, 1 H), 6.44 (d, 1 H), 6.31 (t, 1 H), 5.13 (s, 2 H), 4.77 (s, 2 H), 4.34 (t, 2 H), 4.00 (s, 2 H), 3.84 (t, 2 H). Example 47: Preparation of ethyl 3-(5-chloro-3-((5-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol-l -yl)propanoate
Figure imgf000113_0003
ethyl 3 (5 chioro 3 ^5 ^ ((2 oxopyndin -| (2H) yl)methy|)benzy|jnicotinamidOjmethy|) 1H jndazoi 1 y|jpropanoate
Figure imgf000114_0001
The mixture of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)nicotinamide HC1 salt (260 mg, 0.5 mmol, 1.0 eq), ethyl acrylate (250 mg, 2.5 mmol, 5 eq) and CS2CO3 (815 mg, 2.5 mmol, 5.0 eq) in DMF (3 mL) was was stirred at 80 °C overnight. The mixture was cooled to rt and poured into water (10 mL). The aqueous layer was extracted with DCM (10 mL X3).The combined organic layers were dried over anhydrous Na2S04 and concentrated and the residue was purified via prep-HPLC to afford ethyl 3-(5-chloro-3-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol-l -yl)propanoate as a white solid (47 mg, 16.1%). LRMS (M+H+) m/z calculated 584.2, found 584.1. ¾ NMR (CD30D, 400 MHz) δ 8.83 (d, 1 H), 8.57 (d, 1 H), 8.09 (t, 1 H), 7.85 (d, 1 H), 7.69 (dd, 1 H), 7.62 (s, 1 H), 7.60 (s, 1 H), 7.56-7.51 (m, 1 H), 7.39 (dd, 1 H), 7.26 (dd, 4 H), 6.58 (d, 1 H), 6.40 (t-d, 1 H), 5.18 (s, 2 H), 4.89 (s, 2 H), 4.65 (t, 2 H), 4.08 (s, 2 H), 4.03 (q, 2 H), 2.96 (t, 2 H), 1.11 (t, 3 H).
Example 48: Preparation of 3-(5-chloro-3-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)- 1 H-indazol- 1 -yl)propanoic acid
Figure imgf000114_0002
To the solution of ethyl 3-(5-chloro-3-((5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)methyl)- 1 H-indazol- 1 -yl)propanoate (30 mg, 0.05 mmol, 1 eq) in THF/MeOH (1 mL/1 mL) was added NaOH aqueous solution (1 N, 1 mL) and the mixture was stirred at rt overnight, then concentrated. The residue was acidified with HC1 to pH 5. Filtered and the solid was collected to afford 3-(5-chloro-3-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol-l -yl)propanoic acid as a white solid (27 mg, 93.1 %). LRMS (M+H+) m/z calculated 556.2, found 556.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.39 (t, 1 H), 8.92 (d, 1 H), 8.69 (d, 1 H), 8.20 (s, 1 H), 7.89 (d, 1 H), 7.75 (dd, 1 H), 7.71 (d, 1 H), 7.42-7.38 (m, 2 H), 7.23 (dd, 4 H), 6.58 (d, 1 H), 6.38 (d, 1 H), 6.21 (t, 1 H), 5.04 (s, 2 H), 4.76 (d, 2 H), 4.55 (t, 2 H), 4.03 (s, 2 H), 2.85 (t, 2 H).
Example 49: Preparation of ethyl 3-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)- 1 H-indazol- 1 -yl)propanoate
Figure imgf000115_0001
The mixture of N-((5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamide (68 mg, 0.14 mmol, 1.0 eq), ethyl acrylate (17 mg, 0.17 mmol, 1.2 eq) and CS2CO3 (55 mg, 0.17 mmol, 1.2 eq) in DMF (5 mL) was stirred at 80 °C for 1 h. The mixture was cooled to rt and purified directly by flash chromatography to afford ethyl 3-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)nicotinamido)methyl)-l H-indazol- 1 -yl)propanoate as a yellow solid (65 mg, 79%). LRMS (M+H+) m/z calculated 585.2, found 585.2. ¾ NMR (CD3OD, 400 MHz) δ 8.86 (s, IH), 8.60 (s, IH), 8.44 (s, IH), 8.09 (s, IH), 7.84 (s, IH), 7.75 (d, IH), 7.64 (d, IH), 7.59-7.53 (m, 2H), 7.36 (d, IH), 7.24 (d, IH), 6.55 (d, IH), 6.42 (t, IH), 5.23 (s, 2H), 4.87 (s, 2H), 4.63 (t, 2H), 4.09 (s, 2H), 4.02 (q, 2H), 2.94 (t, 2H), 1.11 (t, 3H).
Example 50: Preparation of 3-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)- 1 H-indazol- 1 -yl)propanoic acid
Figure imgf000116_0001
The solution of ethyl 3-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)-lH-indazol-l-yl)propanoate (76 mg, 0.14 mmol, 1.0 eq) in MeOH (2 mL) was treated with an aqueous solution of NaOH (22 mg, 0.56 mmol, 4.0 eq) in 2 mL of water. The mixture was stirred at rt overnight. Then the mixture was concentrated and the residue was acidified with 1 N HCl till pH was 3. The mixture was purified by flash chromatography to give 3-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)nicotinamido)methyl)-lH-indazol-l-yl)propanoic acid as a white solid (58 mg,
74%). LRMS (M+H+) m/z calculated 557.17, found 557.2. ¾ NMR (CD3OD, 400 MHz) δ 8.85 (s, IH), 8.60 (s, IH), 8.44 (s, IH), 8.09 (s, IH), 7.82 (s, IH), 7.75 (dd, IH), 7.65 (dd, IH), 7.58 (d, IH), 7.54 (td, IH), 7.35 (dd, IH), 7.24 (d, IH), 6.55 (d, IH), 6.41 (td, IH), 5.24 (s, 2H), 4.87 (t, 2H), 4.61 (t, 2H), 4.11 (s, 2H), 2.87 (t,
2H).
Example 51: Preparation of N-((l-(3-amino-3-oxopropyl)-5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2- oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide
Figure imgf000116_0002
N ((1 (3 amino 3 oxopropyh 5 chioro H indazoi 3 ykmethyh 5 ((6 i(2 oxopyric|in ,2H\ yhmethyhpyridin 3 yl)methyl)iiicotinamid6
Figure imgf000117_0001
A mixture of 3-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)-lH-indazol-l -yl)propanoic acid (140 mg, 0.25 mmol, 1.0 eq), NH4CI (20 mg, 0.38 mmol, 1.5 eq), HATU (190 mg, 0.5 mmol, 2.0 eq) and DIEA (96 mg, 0.75 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt overnight. The mixture was poured into water (20 mL) and extracted with EtOAc (30 mL x2). The combined organic layer was washed with water, brine and dried over anhydrous sodium sulfate. The mixture was filtered and concentrated. The obtained residue was purified by flash columm to afford N-((l -(3- amino-3 -oxopropyl)-5-chloro- 1 H-indazol-3 -yl)methyl)-5-((6-((2-oxopyridin-l (2H)-yl)methyl)pyridin-3 - yl)methyl)nicotinamide as a white solid (91.9 mg, 66%).LRMS (M+H+) m/z calculated 556.19, found 556.2. ¾ NMR (DMSO-de, 300 MHz) δ 9.30 (t, 1H), 8.87 (s, 1H), 8.65 (s, 1H), 8.47 (s, 1H), 8.07 (s, 1H), 7.88 (s, 1H), 7.75-7.63 (m, 3H), 7.46-7.37 (m, 3H), 7.13 (d, 1H), 6.87 (s, 1H), 6.36 (d, 1H), 6.23 (t, 1H), 5.12 (s, 2H), 4.75 (d, 2H), 4.54 (t, 2H), 4.03 (s, 2H), 2.66 (t, 2H).
Example 52: Preparation of N-((5-chloro-l -methyl-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000117_0002
A solution of 2-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)isoindoline-l,3-dione (650 mg, 1.6 mmol, 1.0 eq) in MeOH (10 mL) and HC1/EA (3 N, 20 mL) was stirred at rt for 5 h. Then the mixture was concentrated in vacuo to afford 2-((5-chloro-lH-indazol-3-yl)methyl)isoindoline-l,3-dione as a white solid (500 mg, 87%).
Figure imgf000118_0001
To a cold suspension of 2-((5-chloro-lH-indazol-3-yl)methyl)isoindoline-l,3-dione (500 mg, 1.4 mmol, 1.0 eq) and K2C03 (396 mg, 2.8 mmol, 2.0 eq) in DMF (5 mL) was added methyl iodide (408 mg, 2.8 mmol, 2.0 eq). The suspension was stirred at rt overnight and then quenched with EA/H2O (20 mL/20 mL). The precipitate was filtered and dried to afford 2-((5-chloro-l-methyl-lH-indazol-3-yl)methyl)isoindoline-l,3- dione as a white solid (295 mg, 63%).
Figure imgf000118_0002
2-((5-Chloro-l -methyl- lH-indazol-3-yl)methyl)isoindo line- 1, 3 -dione (290 mg, 0.89 mmol, 1.0 eq) in ethanol (20 mL) was treated with hydrazine hydrate (1 mL, 31.5 mmol), the mixture was stirred at rt for 20 h. The solid precipitate was filtered off and the filtrate was concentrated in vacuo. The residue was purified by flash chromatography (MeCN/l hO = 1/2 to 9/1) to give (5-chloro-l-methyl-lH-indazol-3-yl)methanamine (162 mg, 93%).
Figure imgf000118_0003
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (150 mg, 0.46 mmol, 1.0 eq), (5- chloro-1 -methyl- lH-indazol-3-yl)methanamine (160 mg, 0.81 mmol, 1.7 eq), HATU (213 mg, 0.56 mmol, 1.2 eq) and DIEA (1 mL) in DMF (3 mL) was stirred at rt for 2 h. The mixture was purified by flash
chromatography (MeCN/ H20 = 1/2 to 9/1) to afford N-((5-chloro-l-methyl-lH-indazol-3-yl)methyl)-5-(4- ((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (112 mg, 48%>). LRMS (M+H+) m/z calculated 498.2, found 498.2. ¾ NMR (DMSO-d6, 300 MHz) δ 9.28 (t, IH), 8.85 (d, IH), 8.61 (d, IH), 8.03 (t, IH), 7.90 (d, IH), 7.74 (dd, IH), 7.65 (d, IH) , 7.42-7.36 (m, 2H), 7.24-7.18 (m, 4H), 6.38 (d, IH), 6.22- 6.17 (m, IH), 5.02 (s, 2H), 4.74 (d, 2H), 4.00 (s, 3H), 3.98 (s, 2H).
Example 53: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)sulfonyl)benzamide
Figure imgf000119_0001
N"((5"c |oro"i H^dazof3" |)met yl)"3"((4 "(^^
Figure imgf000119_0002
To a solution of 3-mercaptobenzoic acid (0.5 g, 3.3 mmol, 1.0 eq) in DMF (5 mL) was added EtONa (2.1 g, 21% in EtONa, 6.5 mmol, 2.0 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, then 4- fluorobenzaldehyde (0.4 g, 3.3 mmol, 1.0 eq) was added into the mixture. The reaction mixture was stirred at rt overnight. The reaction mixture was poured into ice-water (10 mL), acidified with 1 N HCl to pH 3-4. The mixture was concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (20 mL) and filtered. The filtrate was concentrated under reduced pressure. The residue was purified via flash chromatography to afford 3-((4-formylphenyl)thio)benzoic acid as a yellow solid (1.1 g, about 50%> purity, 64%).
Figure imgf000119_0003
To a solution of 3-((4-formylphenyl)thio)benzoic acid (1.1 g, about 50%> purity, 2.2 mmol, 1.0 eq) in MeOH (10 mL) was added H2SO4 (0.84 g, 8.6 mmol, 4.0 eq) at rt. The mixture was heated at 80 °C overnight. The mixture was concentrated to remove MeOH, then diluted with ice -water (10 mL) and neutralized with saturated NaHC03 to pH 7-8. The mixture was concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (20 mL) and filtered. The filtrate was concentrated under reduced pressure. The residue was purified via flash chromatography to afford methyl 3-((4-formylphenyl)thio)benzoate as a yellow solid (400 mg, 68%).
Figure imgf000119_0004
To a solution of methyl 3-((4-formylphenyl)thio)benzoate (400 mg, 1.47 mmol, 1.0 eq) in EtOH (10 mL) was added NaB¾ (67 mg, 1.76 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at rt overnight. The mixture was mixed with water (5 mL). The mixture was concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (20 mL) and filtered. The filtrate was concentrated under reduced pressure. The residue was purified via flash chromatography to afford methyl 3-((4- (hydroxymethyl)phenyl)thio
Figure imgf000120_0001
To a solution of methyl 3-((4-(hydroxymethyl)phenyl)thio)benzoate (200 mg, 0.73 mmol, 1.0 eq) in DCM (2 mL) were added SOCb (434 mg, 3.65 mmol, 5.0 eq) at 0 °C under N2. The mixture was stirred at rt for 30 min. The mixture was concentrated to dryness under reduced pressure to afford methyl 3-((4- (chloromethyl)phenyl)thio)
Figure imgf000120_0002
A mixture of methyl 3-((4-(chloromethyl)phenyl)thio)benzoate (200 mg crude, 0.73 mmol, 1.0 eq), pyridin- 2(lH)-one (104 mg, 1.1 mmol, 1.5 eq) and K2CO3 (302 mg, 2.19 mmol, 3.0 eq) in MeCN (10 mL) was stirred at 80 °C overnight. The mixture was filtered and the filtrate was concentrated to afford methyl 3-((4-((2- oxopyridin-l(2H)-yl)me .
Figure imgf000120_0003
To a solution of methyl 3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)thio)benzoate (300 mg crude, 0.73 mmol, 1.0 eq) in MeOH (5 mL) and H20 (5 mL) was added NaOH (88 mg, 2.19 mmol, 3.0 eq). The mixture was heated at 60 °C for 30 min. The mixture was concentrated to remove MeOH, then diluted with ice-water (10 mL) and acidified with 1 N HCl to pH 3-4. The mixture was concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (10 mL) and filtered. The filtrate was concentrated under reduced pressure. The residue was purified via flash chromatography to afford 3-((4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)thio)benzoic acid as a white solid (120 mg, 49%>).
Figure imgf000121_0001
To a solution of 3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)thio)benzoic acid (110 mg, 0.33 mmol, 1.0 eq) in DCM (10 mL) were added m-CPBA (662 mg, 3.3 mmol, 10.0 eq) at rt. The mixture was stirred at rt for 2 h. The mixture was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford 3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)benzoic acid as a white solid (70 mg, 57%).
Figure imgf000121_0002
To a solution of 3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)benzoic acid (70 mg, 0.19 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (81 mg, 0.63 mmol, 3.0 eq) and HATU (96 mg, 0.25 mmol, 1.3 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol- 3-yl)methanamine (61 mg, 0.23 mmol, 1.2 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)-3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)benzamide as a yellow solid (100 mg, 85%).
Figure imgf000121_0003
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin- l(2H)-yl)methyl)phenyl)sulfonyl)benzamide (100 mg, 0.16 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/MeOH (2 mL) at 0 °C. The mixture was stirred at rt for 2 h. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL), neutralized with saturated NaHC03 (10 mL). The aqueous layer was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)sulfonyl)benzamide as a yellow solid (70 mg, 82%). LRMS (M-H) m/z caculated 531.1, found 531.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.12 (brs, 1 H), 9.44 (t, 1 H), 8.43 (s, 1 H), 8.16 (d, 1 H), 8.08 (d, 1 H), 7.98-7.94 (m, 2 H), 7.90 (s, 1 H), 7.78 (d, 1 H), 7.71 (t, 1 H), 7.55 (d, 1 H), 7.48-7.40 (m, 3 H), 7.33 (dd, 1 H), 6.40 (d, 1 H), 6.25 (t, 1 H), 5.15 (s, 2 H), 4.80 (d, 2 H).
Example 54: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(difluoro(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzamide
Figure imgf000122_0001
N ((5 chioro -in inrfazoi 3 ljimethyl) 3 (dif|uoro(4 ((2 oxopyi-jdin 1(2H) yljmethyl henyijmethyljbenzamjcie
Figure imgf000122_0002
To a solution of dimethyl isophthalate (25 g, 129 mmol, 4.3 eq) in dry THF (500 mL) was added p- tolylmagnesium bromide (30 mmol, 1 eq) dropwise under nitrogen in an ice-water bath and the mixture was stirred at this temperature for 4 h. The reaction mixture was poured into aqueous NH4CI solution and extracted with EA. The organic layers were concentrated and the residue was purified by flash chromatography to give methyl 3-(4-methylbenzoyl)benzoate as a white solid (1.6 g, 21%).
Figure imgf000122_0003
To a solution of methyl 3-(4-methylbenzoyl)benzoate (508 mg, 2 mmol, 1 eq) and BF3.Et20 (1.42 g, 10 mmol, 5 eq) in dry DCM (5 mL) was added ethane- 1,2-dithiol (954 mg, 8 mmol, 4 eq). The mixture was stirred overnight and then partitioned between DCM and water. The organic layer was concentrated and purified by Prep-TLC (PE/EA = 5/1) to afford methyl 3-(2-(p-tolyl)-l,3-dithiolan-2-yl)benzoate as a white solid (400 mg, 61%).
Figure imgf000122_0004
To a solution of NO+BF4- (1 g, 8.5 mmol, 5.7 eq) and 70% HF-pyridine (4.4 g, 31 mmol, 20 eq) in dry DCM (20 mL) at 0 °C was added a solution of methyl 3-(2-(p-tolyl)-l,3-dithiolan-2-yl)benzoate (500 mg, 1.5 mmol, 1 eq) in DCM (5 mL). The mixture was stirred at 0 °C for 1 h, then diluted with DCM and passed through a short column packed with Ab03/Mg2S04 (20 g / 8 g). The collected DCM solution was concentrated, purified by prep-TLC (PE/EA = 15:1) to give methyl 3-(difluoro(p-tolyl)methyl)benzoate as a white oil (320 mg, 77%).
Figure imgf000123_0001
To a solution of methyl 3-(difluoro(p-tolyl)methyl)benzoate (276 mg, 1 mmol, 1 eq) in CCL (10 mL) were added NBS (213 mg, 1.2 mmol, 1.2 eq) and AIBN (16.4 mg, 0.1 mmol, 0.1 eq). The mixture was stirred at 85 °C for 3 h, then concentrated, poured into water, and extracted with DCM (3x20 mL). The combined organic layers were concentrated, purified by prep-TLC (PE/EA = 15: 1) to afford methyl 3-((4- (bromomethyl)phenyl)difluoromethyl)benzoate as a white oil (300 mg, 84.5%).
Figure imgf000123_0002
To a solution of methyl 3-((4-(bromomethyl)phenyl)difluoromethyl)benzoate (200 mg, 0.56 mmol, 1 eq) in MeCN (10 mL) were added pyridin-2(lH)-one (108 mg, 1.1 mmol, 2 eq) and K2C03 (232 mg, 1.68 mmol, 3 eq). The mixture was stirred at 80 °C for 2 d, then concentrated, poured into water, and extracted with EA (3x20 mL). The combined organic layers were washed with brine, concentrated, and purified by prep-TLC (PE/EA = 1 : 1) to afford methyl 3-(difluoro(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)methyl)benzoate as a white oil (90 mg, 43%).
Figure imgf000123_0003
To a solution of methyl 3-(difluoro(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)methyl)benzoate (90 mg, 0.24 mmol, 1.0 eq) in THF/H20 (15 mL/5 mL) was added LiOH.H20 (101 mg, 2.4 mmol, 10.0 eq). The mixture was stirred at rt overnight, and concentrated to give 3-(difluoro(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzoic acid (crude) for the next step.
Figure imgf000124_0001
To a solution of 3-(difluoro(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)methyl)benzoic acid (crude, -0.24 mmol, 1 eq) in DMF (10 mL) were added TEA (48.5 mg, 0.48 mmol, 2 eq), (5-chloro-l -(tetrahydro-2H- pyran-2-yl)-lH-indazol-3-yl)methanamine (63.8 mg, 0.24 mmol, 1 eq) and HATU (182.4 mg, 0.48 mmol, 2 eq). The reaction mixture was stirred at rt for 3 h. The mixture was concentrated, and purified by prep-TLC (EA/Et3N = 50/1) to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-(difluoro(4- ((2-oxopyridin-l(2H)-yl)methyl)phenyl)methyl)benzamide as a white oil (90 mg, 62%).
Figure imgf000124_0002
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-(difluoro(4-((2- oxopyridin-l(2H)-yl)methyl)phenyl)methyl)benzamide (90 mg, 0.15 mmol, 1.0 eq) in MeOH (4 mL) was added HCl/MeOH (4 mL). The mixture was stirred at rt for 12 h and then concentrated. The residue was purified by prep-HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-(difluoro(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzamide as a white solid (60 mg, 77%). LRMS (M+H+) m/z caculated 519.1, found 518.8. ¾ NMR (400 MHz, CD3OD) δ 8.04 (s, 1H), 7.95 (d, 1H), 7.88 (d, 1H), 7.70 (dd, 2H), 7.61-7.46 (m, 5H), 7.37 (m, 3H), 6.59 (d, 1H), 6.42 (q, 1H), 5.24 (s, 2H), 4.92 (s, 2H).
Example 55: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(hydroxy(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzamide
Figure imgf000124_0003
N ((5 chioro 1 H indazoi 3 yi^methylj 3 (hydroxy^ ((2 οχοργ¾η 1 (2H) yljmethyljphenyijmethyljbenzamide
Figure imgf000125_0001
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin- l(2H)-yl)methyl)benzoyl)benzamide (30 mg, 0.1 mmol, 1.0 eq) in CH3OH (5 mL) was added NaB¾ (76 mg, 0.2 mmol, 2.0 eq) at 0 °C. The mixture was stirred at rt overnight, and then concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-3 -(hydroxy(4-((2-oxopyridin- l(2H)-yl)methyl)p
Figure imgf000125_0002
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-(hydroxy(4-((2- oxopyridin-l(2H)-yl)methyl)phenyl)methyl)benzamide (30 mg, 0.051 mmol, 1.0 eq) in MeOH (5 mL) was added MeOH/HCl (4 N, 5 mL). The mixture was stirred at rt overnight and concentrated and purified by prep- HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-(hydroxy(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzamide HC1 salt as a white solid (8.0 mg, 32%). LRMS (M+H+) m/z caculated 499.2, found 499.1. ¾ NMR (CD3OD, 400 MHz) δ 7.90(d, 2 H), 7.75 (m, 2 H), 7.60-7.48 (m, 3 H), 7.40-7.29 (dd, 4H), 7.28 (d, 2 H), 6.64 (d, 2 H), 6.49 (t, 1 H), 5.82 (s, 1H), 5.21 (s, 2 H), 4.92 (s, 2 H).
Example 56: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(methoxy(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzamide
Figure imgf000125_0003
N"((5"c |oro"i H^dazo| y|)met y|) (met^
Figure imgf000126_0001
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-(hydroxy(4-((2- oxopyridin-l(2H)-yl)methyl)phenyl)methyl)benzamide (29 mg, 0.05 mmol, 1.0 eq) in dry DCM under N2 was added DAST (0.05 mL) dropwise at -65 °C. The mixture was stirred at -65 °C for 1 h. The reaction mixture was quenched with aqueous NH4CI, diluted with water, extracted with DCM (3x20 mL). The combined organic layers were concentrated, purified by prep-TLC (DCM/MeOH = 30/1) to afford N-((5-chloro-l - (tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-3 -(fluoro(4-((2-oxopyridin-l (2H)- yl)methyl)p
Figure imgf000126_0002
To a stirred suspension of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-3-(fluoro(4-
((2-oxopyridin-l(2H)-yl)methyl)phenyl)methyl)benzamide (20 mg, 0.034 mmol, 1.0 eq) in MeOH (2 mL) was added MeOH/HCl (4 N, 1 mL). The mixture was stirred at rt for 5 h. Then it was concentrated and rinsed with MeCN to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-(methoxy(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzamide HC1 salt as a white solid (10 mg, 47%). LRMS (M+H+) m/z caculated 513.2, found 513.1. ¾ NMR (CD3OD, 400 MHz) δ 7.76 (s, 2 H), 7.56 (dd, 2 H), 7.40-7.35(m, 3 H), 7.29-7.21 (m, 4 H), 7.13 (m, 2 H), 6.44 (d, 1 H), 6.25 (t, 1 H), 5.21 (s, 1 H), 5.04 (s, 2 H) , 3.21 (s, 3 H).
Example 57: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(l -hydroxy-l-(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl) ethyl)benzamide
Figure imgf000126_0003
N"((5"ch|oro"i H^dazof3"y|)methy|)"3"^^
Figure imgf000127_0001
To a solution of N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)- yl)methyl)benzoyl)benzamide (30 mg, 0.060 mmol, 1 eq) in dry THF (3 mL) was added MeMgBr (3.0 M in THF, 0.12 mL) dropwise at 0 °C under N2 and the mixture was stirred at rt overnight. The reaction mixture was quenched with aqueous NH4CI, diluted with water, extracted with EtOAc (3x20 mL). The combined organic layers was concentrated to afford N-((5-chloro-lH-indazol-3-yl)methyl)-3-(l-hydroxy-l -(4-((2- oxopyridin-l(2H)-yl)methyl)phenyl)ethyl)benzamide as a white solid (9.5 mg, 30 %). LRMS (M+H+) m/z caculated 513.2, found 513.2. ¾ NMR (CD3OD, 400 MHz) 87.90 (s, 1 H), 7.89 (d, 1 H), 7.69 (dd, 2 H), 7.59- 7.48 (m, 3 H), 7.42-7.34 (m, 4H), 7.24 (d, 2 H), 6.57 (d, 1 H), 6.37 (t, 1 H), 5.17 (s, 2H), 4.91 (s, 2 H), 1.92 (s, 3 H).
Example 58: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-fluoro-4-((2-oxopyridin-l yl)methyl)benzyl)nicotinamide
Figure imgf000127_0002
A mixture of methyl 4-(bromomethyl)-3-fluorobenzoate (500 mg, 2.02 mmol, 1.0 eq), pyridin-2(lH)-one (384 mg, 4.04 mmol, 2.0 eq) and K2CO3 (836 mg, 6.06 mmol, 3.0 eq) in MeCN (5 mL) was stirred at 85 °C overnight. The mixture was filtered and the filtrate was concentrated. The residue was purified via flash chromatography to afford methyl 3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate as a yellow solid (430 mg, 82%).
Figure imgf000128_0001
To a solution of methyl 3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate (380 mg, 1.46 mmol, 1.0 eq) in THF (5 mL) was added NaBFL (332 mg, 8.74 mmol, 6.0 eq). The mixture was heated at 60 °C. The mixture was added dropwise MeOH (5 mL) over 1 h. The mixture was heated at 60 °C for 5 h. The mixture was added water (10 mL). The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated to afford l-(2-fluoro-4-(hydroxymethyl)benzyl)pyridin- 2(lH)-one as a white solid (350
Figure imgf000128_0002
To a solution of l-(2-fluoro-4-(hydroxymethyl)benzyl)pyridin-2(lH)-one (350 mg crude, 1.46 mmol, 1.0 eq) in DCM (5 mL) were added PBr3 (593 mg, 2.19 mmol, 1.5 eq) at rt. The mixture was stirred at rt for 1 h. The mixture was mixed with saturated NaHC03 (10 mL) and extracted with DCM (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford l-(4-(bromomethyl)-2-fluorobenzyl)pyridin-2(lH)-one as a white solid (170 mg, 39%).
Figure imgf000128_0003
To a solution of ethyl 5-bromonicotinate (336 mg, 1.46 mmol, 1.0 eq) in 1,4-dioxane (10 mL) were added AcOK (420 mg, 4.38 mmol, 3.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2-dioxaborolane) (371 mg, 1.46 mmol, 1.0 eq) and Pd(dppf)Cl2 (54 mg, 0.07 mmol, 0.05 eq). The mixture was heated at 85 °C overnight under N2, and then the mixture was cooled to rt, to the mixture were added l-(4-(bromomethyl)-2- fluorobenzyl)pyridin-2(lH)-one (432 mg, 1.46 mmol, 1.0 eq), Pd(dppf)Cl2 (54 mg, 0.07 mmol, 0.05 eq) and Na2C03 (464 mg, 4.38 mmol, 3.0 eq) in water (5 mL). The mixture was heated to 95 °C for 2 h. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL) and extracted with DCM (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford ethyl 5-(3-fluoro-4- ((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate as a white solid (100 mg, 48%).
Figure imgf000129_0001
To a solution of ethyl 5-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate (100 mg, 0.27 mmol, 1.0 eq) in MeOH (2 mL) and H20 (2 mL) was added NaOH (32 mg, 0.81 mmol, 3.0 eq). The mixture was heated at 60 °C for 30 min. The mixture was concentrated to remove MeOH, then diluted with ice-water (10 mL) and acidified with 1 N HCl to pH 2-3. The mixture was concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (10 mL) and filtered. The filtrate was concentrated under reduced pressure to afford 5-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid as a yellow solid (150 mg crude, quant).
Figure imgf000129_0002
To a solution of 5-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (150 mg crude, 0.27 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (104 mg, 0.81 mmol, 3.0 eq) and HATU (123 mg, 0.32 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l-(tetrahydro-2H-pyran- 2-yl)-lH-indazol-3-yl)methanamine (79 mg, 0.3 mmol, 1.1 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)-5-(3-fluoro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)nicotinamide as a white solid (120 mg, 76%).
Figure imgf000129_0003
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(3-fluoro-4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (120 mg, 0.2 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/EA (2 mL) at 0 °C. The mixture was stirred at rt overnight. The mixture was filtered. The filtered cake was washed with Et20 (10 mL). The filtered cake was dryness under reduced pressure to afford N-((5-chloro- lH-indazol-3-yl)methyl)-5-(3-fluoro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)nicotinamide HC1 salt as a white solid (70 mg, 65%). LRMS (M+H+) m/z caculated 502.1, found 502.1. ¾ NMR (DMSO-i/6, 400 MHz) δ 13.16 (brs, 1 H), 9.66 (t, 1 H), 9.13 (s, 1 H), 8.93 (s, 1 H), 8.66 (s, 1 H), 7.94 (d, 1 H), 7.71 (dd, 1 H), 7.54 (d, 1 H), 7.50-7.40 (m, 1 H), 7.34 (dd, 1 H), 7.24 (d, 1 H), 7.14 (d, 1 H), 7.08 (t, 1 H), 6.39 (d, 1 H), 6.24 (t, 1 H), 5.07 (s, 2 H), 4.81 (d, 2 H), 4.16 (s, 2 H).
Example 59: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-chloro-4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000130_0001
To a suspension of methyl 4-(bromomethyl)-3-chlorobenzoate (500 mg, 1.8 mmol, 1.0 eq) and pyridin-2(lH)- one (360 mg, 3.8 mmol, 2.0 eq) in MeCN (10 mL) was added K2C03 (523 mg, 3.8 mmol, 2.0 eq). The mixture was heated to 85 °C overnight. After cooled to rt, the mixture was concentrated in vacuo to give a solid, whihc was purified by flash chromatography to afford methyl 3-chloro-4-((2-oxopyridin-l (2H)-yl)methyl)benzoate as a white solid (413 mg, 78%
Figure imgf000130_0002
To a solution of methyl 3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate (410 mg, 1.4 mmol, 1.0 eq) in THF (20 mL) was added L1BH4 (325 mg, 14 mmol, 10.0 eq) carefully. After addition, the mixture was heated to reflux for 1 h and allowed to cool. The mixture was quenched with water (20 mL), extracted with EA (20 mL x 3), the combined organic layer was washed with brine, dried over Na2S04, filtered and concentrated in vacuo to afford l-(2-chloro-4- llow oil (352 mg, 95%).
Figure imgf000131_0001
To a solution of l-(2-chloro-4-(hydroxymethyl)benzyl)pyridin-2(lH)-one (350 mg, 1.4 mmol, 1.0 eq) in DCM (10 mL) was added drop wise SOCh (1 mL) in ice-water bath. After addition, the mixture was stirred at rt for 1 h. After removal of the solvent, the residue was purified by flash chromatography to afford 1 -(2-chloro-4- (chloromethyl)benzyl)
Figure imgf000131_0002
To a suspension of ethyl 5-bromonicotinate (1.0 g, 4.3 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'- bi(l,3,2-dioxaborolane) (1.1 g, 4.3 mmol, 1.0 eq) and AcOK (1.280 g, 13 mmol, 3.0 eq) in dioxane (50 mL) was added Pd(dppf)Ci2 (160 mg, 0.21 mmol, 0.05 eq). The mixture was heated to 85 °C under N2 overnight. After cooled to rt, l-(2-chloro-4-(chloromethyl)benzyl)pyridin-2(lH)-one (360 mg, 1.3 mmol, 0.3 eq), and a solution of Na2CC>3 (1.380 g, 13 mmol, 3 eq) in water (8 mL) were added, followed by Pd(dppf)Ci2 (160 mg, 0.21 mmol, 0.05 eq). The mixture was then heated to 95 °C for a further 2 h and allowed to cool. After removal of the solvent, the residue was quenched with water (30 mL), extracted with EA (3x30 mL), the combined organic layer was washed with brine dried over Na2S04, filtered and concentrated in vacuo to give an oil, which was further purified by chromatography on silica gel column, elution with DCM/MeOH (30/1 to 10/1) to afford ethyl 5-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate as a yellow solid (399 mg, crude).
Figure imgf000131_0003
To a solution of ethyl 5-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate (399 mg, crude, 1.0 eq) in MeOH (10 mL) and H20 (10 mL) was added NaOH (400 mg, 10.0 mmol). The mixture was stirred at 75 °C for 2 h. The organic solvents were evaporated. To the residue was added cone. HC1 till pH 3. The precipitate was filtered and dried to afford 5-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid as a white solid (260 mg, 54%> of above two steps).
Figure imgf000132_0001
A mixture of 5-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (260 mg, 0.73 mmol, 1.0 eq), (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (195 mg, 0.73 mmol, 1.0 eq), HATU (279 mg, 0.73 mmol, 1.0 eq) and DIEA (1 mL) in DCM (20 mL) was stirred at rt for 2 h, the mixture was diluted with DCM (50 mL), washed with saturated NaHCCb (25 mL), brine, dried over Na2SC>4, filtered and concentrated in vacuo. The residue was then further purified by flash chromatography (MeCN/l hO = 25%- 95%) to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(3-chloro-4-((2- oxopyridin-l(2H)
Figure imgf000132_0002
A solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(3-chloro-4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (310 mg, 0.51 mmol, 1.0 eq) in MeOH (5 mL) and HC1/EA (3 N, 10 mL) was stirred at rt for 20 h. Then the mixture was concentrated. The residue was purified by flash chromatography (MeCN/H20 = 25%- 95%) to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-chloro-4- ((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (76 mg, 26%>). LRMS (M+H+) m/z calculated 518.1, found 518.1. ¾ NMR (DMSO-i/6, 300 MHz) δ 13.07 (s, 1H), 9.28 (t, 1H), 8.87 (d, 1H),
8.64 (d, 1H), 8.06 (t, 1H), 7.90 (d, 1H), 7.65 (m, 1H), 7.54-7.43 (m, 3H) , 7.33 (m, 1H), 7.21 (m, 1H), 6.81 (d, 1H), 6.42 (d, 1H), 6.25 (m, 1H), 5.10 (s, 2H), 4.78 (d, 2H), 4.01 (s, 2H).
Example 60: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-fluoro-4-((2-oxopyridin-l yl)methyl)benzyl)nicotinamide
Figure imgf000132_0003
N ((5 chioro 1 H indazoi 3 y^methyl) 5 (2 fiuoro 4 ((2 oxopyndin 1 (2H) y^methyl^enzyi^icotinamide
Figure imgf000133_0001
To a solution of benzyl 5-bromonicotinate (1.0 g, 3.42 mmol, 1.0 eq) in 1,4-dioxane (10 mL) were added AcOK (985 mg, 10.26 mmol, 3.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l ,3,2-dioxaborolane) (870 mg, 3.42 mmol, 1.0 eq) and Pd(dppf)Cl2 (125 mg, 0.17 mmol, 0.05 eq). The mixture was heated at 85 °C overnight under N2, and then the mixture was cooled to rt, to the mixture were added methyl 4-(bromomethyl)-3- fluorobenzoate (845 mg, 3.42 mmol, 1.0 eq), Pd(dppf)Cl2 (125 mg, 0.17 mmol, 0.05 eq) and Na2C03 (1.09 g, 10.26 mmol, 3.0 eq) in water (5 mL). The mixture was heated to 95 °C for 2 h. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford benzyl 5-(2-fluoro-4- (methoxycarbonyl)benzyl)nicotinate as a yellow oil (360 mg, 28%).
Figure imgf000133_0002
To a solution of benzyl 5-(2-fluoro-4-(methoxycarbonyl)benzyl)nicotinate (360 mg, 0.95 mmol, 1.0 eq) in MeOH (5 mL) was added Pd/C (36 mg, 10% Pd). The mixture was hydrogenated at rt overnight. The mixture was filtered and the filtered cake was washed with DCM (10 mL) and MeOH (10 mL). The filtrate was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford 5-(2-fluoro-4-(metho ant).
Figure imgf000133_0003
To a solution of 5-(2-fluoro-4-(methoxycarbonyl)benzyl)nicotinic acid (278 mg crude, 0.95 mmol, 1.0 eq) in DMF (5 mL) were added DIPEA (368 mg, 2.85 mmol, 3.0 eq) and HATU (433 mg, 1.14 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methanamine (278 mg, 1.05 mmol, 1.1 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford methyl 4-((5-(((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl) methyl)carbamoyl)pyridin-3-yl)methyl)-3-fluorobenzoate as an off-white solid (360 mg, 71%).
Figure imgf000134_0001
To a solution of methyl 4-((5-(((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)carbamoyl)pyridin-3-yl)methyl)-3-fluorobenzoate (360 mg, 0.67 mmol, 1.0 eq) in THF (10 mL) was added L1BH4 (147 mg, 6.7 mmol, 10.0 eq). The mixture was heated at 60 °C overnight. The mixture was added water (5 mL). The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl) methyl)-5-(2-fluoro-4- (hydroxymethyl)benzyl)nicotinamide as a yellow solid (160 mg, 47%).
Figure imgf000134_0002
To a solution of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(2-fluoro-4- (hydroxymethyl)benzyl)nicotinamide (140 mg, 0.28 mmol, 1.0 eq) in DCM (2 mL) were added Et3N (28 mg, 0.28 mmol, 1.0 eq) and MsCl (32 mg, 0.28 mmol, 1.0 eq) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with saturated NaHCCb (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl) methyl)-5-(4-(chloromethyl)-2- fluorobenzyl)nic
Figure imgf000134_0003
A mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-(chloromethyl)-2- fluorobenzyl)nicotinamide (20 mg, 0.038 mmol, 1.0 eq), pyridin-2(lH)-one (18 mg, 0.19 mmol, 5.0 eq) and K2CO3 (16 mg, 0.11 mmol, 3.0 eq) in MeCN (2 mL) was stirred at 80 °C overnight. The mixture was filtered and the filtrate was concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l- (tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-5-(2-fluoro-4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)nicotinamide as a yellow solid (13 mg, 46%).
Figure imgf000135_0001
To a solution of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(2-fluoro-4-((2- oxopyridin-l (2H)-yl)methyl)benzyl)nicotinamide (13 mg, 0.022 mmol, 1.0 eq) in MeOH (2 mL) was added HC1/EA (2 mL) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was added saturated NaHCC (10 mL) and concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (10 mL), filtered. The filtrate was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford N-((5-chloro- lH-indazol-3-yl)methyl)-5-(2-fluoro-4-((2-oxopyridin-l (2H)-yl) methyl)benzyl)nicotinamide as a white solid (7 mg, 64%). LRMS (M+H+) m/z caculated 502.1 , found 502.1. ¾ NMR (CO3OO-d4, 400 MHz) δ 8.72 (d, 1 H), 8.45 (s, 1 H), 7.97 (s, 1 H), 7.75 (d, 1 H), 7.59 (dd, 1 H), 7.46-7.40 (m, 1 H), 7.38 (d, 1 H), 7.24 (dd, 1 H), 7.17 (t, 1 H), 6.96 (t, 2 H), 6.46 (d, 1 H), 6.29 (td, 1 H), 5.05 (s, 2 H), 4.79 (s, 2 H), 3.97 (s, 2 H). Example 61: Preparation ofN-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-cyano-4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000135_0002
N ((5 chioro 1 H indazoi 3 y^meth l) 5 (3 cyano 4 ((2 oxopyndin 1 (2H) y^methyl^enzyi^icotinamide
Figure imgf000135_0003
A mixture of methyl 3-iodo-4-methylbenzoate (2 g, 7.2 mmol, 1.0 eq) and CuCN (774 mg, 8.6 mmol, 1.2 eq) in NMP (20 mL) was stirred at 200 °C wiht microwave for 1 h. Then the mixture was poured into water (50 mL), extracted with EtOAc (50 mL x3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified on silica gel column (PE/EtOAc = 5/1) to afford methyl 3-cyano-4-methylbenzoate as a white solid (1.2 g, 95%>).
Figure imgf000136_0001
A mixture of methyl 3-cyano-4-methylbenzoate (1.7 g, 9.7 mmol, 1.0 eq), NBS (2.07 g, 11.6 mmol, 1.2 eq) and BPO (234 mg, 0.97 mmol, 0.1 eq) in CC14 (20 mL) was stirred at 90 °C under N2 overnight. Then the reaction was quenched with water (10 mL), extracted with DCM (50 mL x3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified on silica gel column (PE/EtOAc = 20/1) to afford methyl 4-(bromomethyl)-3-cyanobenzoate as a yellow solid
(1.5 g, 61%).
Figure imgf000136_0002
A mixture of methyl 4-(bromomethyl)-3-cyanobenzoate (1.5 g, 5.9 mmol, 1.0 eq), pyridin-2(lH)-one (842 mg, 8.9 mmol, 1.5 eq) and K2CO3 (1.63 g, 11.8 mmol, 2.0 eq) in CH3CN (30 mL) was stirred at 85 °C overnight. After cooled to rt, the mixture was filtered. The filtrate was concentrated. The residue was purified on silica gel column (PE/EtOAc = 1/1) to afford methyl 3-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate as a yellow solid (1.2 g, 75%).
Figure imgf000136_0003
To a solution of methyl 3-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate (1 g, 3.7 mmol, 1.0 eq) in THF (30 mL) was added L1BH4 (810 mg, 37 mmol, 10.0 eq). The mixture was refiuxed and stirred for 1 h. LCMS showed the reaction complete. Then the mixture was cooled to rt and dropped to water. The obtained mixture was extracted with EtOAc (50 mL *3). The mixture was washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified on silica gel column (PE/EtOAc = 1/2) to give 5- (hydroxymethyl)-2-((2-oxopyridin-l(2H)-yl)methyl)benzonitrile as a yellow oil (720 mg, 81%).
Figure imgf000136_0004
The mixture of 5-(hydroxymethyl)-2-((2-oxopyridin-l(2H)-yl)methyl)benzonitrile (620 mg, 2.58 mmol, 1.0 eq) in DCM (5 mL) and SOCk. (5 mL) was stirred at rt overnight. Then the mixture was concentrated. The residue was purified on silica gel column (PE/EtOAc = 5/1) to give 5-(chloromethyl)-2-((2-oxopyridin-l(2H)- yl)methyl)benzonitrile as a yellow oil (420 mg, 63%).
Figure imgf000137_0001
The mixture of ethyl 5-bromonicotinate (374 mg, 1.6 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'- bi(l,3,2-dioxaborolane) (406 mg, 1.6 mmol, 1.0 eq), Pd(dppf)Ck (58 mg, 0.05 eq) and KOAc (470 mg, 4.8 mmol, 3.0 eq) in dioxane (15 mL) was degassed with N2 and stirred at 85 °C overnight. Then the mixture was cooled, 5-(chloromethyl)-2-((2-oxopyridin-l(2H)-yl)methyl)benzonitrile (420 mg, 1.6 mmol, 1.0 eq), Pd(dppf)Ci2 (58 mg, 0.05 eq) and an aqueous solution of Na2CC>3 (509 mg, 4.8 mmol, 3.0 eq) in 8 mL of water were added. The mixture was degassed with N2 and stirred at 95 °C overnight. Then the mixture was cooled and concentrated, the residue was dissolved in EtOAc (150 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via silica gel column (DCM/EtOAc = 1/1) to afford ethyl 5-(3-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate as a yellow solid (410 mg, 69%).
Figure imgf000137_0002
The solution of ethyl 5-(3-cyano-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)nicotinate (410 mg, 1.1 mmol, 1.0 eq) in MeOH (10 mL) was treated with an aqueous solution of LiOH (92 mg, 2.2 mmol, 2.0 eq) in 5 mL of water. The mixture was stirred for 2 h at rt. Then the mixture was concentrated and the residue was acidified with 1 N HC1 till pH 3. The mixture was concentrated to give 5-(3-cyano-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinic acid as a yellow solid (520 mg, quant). The solid was used for the next step without further purific
Figure imgf000137_0003
A mixture of (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (292 mg, 1.1 mmol, 1.0 eq), 5-(3-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (380 mg, 1.1 mmol, 1.0 eq), HATU (627 mg, 1.65 mmol, 1.5 eq) and DIEA (426 mg, 3.3 mmol, 3.0 eq) in DMF (20 mL) was stirred at rt for 2 h. The mixture was poured into water (50 mL). The appeared solid was filtered and dried to afford N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3-yl)methyl)-5-(3 -cyano-4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)nicotinamide as a brown solid (550 mg, 84%). The solid was used for the next step without further purificatio
Figure imgf000138_0001
The solutuion of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(3-cyano-4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (550 mg, 0.93 mmol, 1.0 eq) in 1 N HCl/EtOAc was stirred at rt overnight. Then the mixture was concentrated and the residue was washed with MeOH to give N-((5- chloro-lH-indazol-3-yl)methyl)-5-(3-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide HCl salt as a white solid (382 mg, 81%). LRMS (M+H+) m/z calculated 509.1, found 509.1. ¾ NMR (CD3OD, 400 MHz) δ 9.20 (d, 1H), 8.95 (s, 1H), 8.91 (s, 1H), 7.92 (s, 1H), 7.85 (dd, 1H), 7.79 (d, 1H), 7.67-7.61 (m, 2H), 7.52 (d, 2H), 7.39 (dd, 1H), 7.28 (d, 1H), 6.62 (d, 1H), 6.54 (t, 1H), 5.41 (s, 2H), 4.97 (s, 2H), 4.36 (s, 2H).
Example 62: Preparation of 5-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro- 1H- indazol-3-yl)methyl)nicotinamide
Figure imgf000138_0002
A mixture ofN-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-cyano-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide (150 mg, 0.3 mmol, 1.0 eq) and K2CO3 (41 mg, 0.3 mmol, 1.0 eq) in DMSO (3 mL) was added H2O2 (3 mL) dropwise. Then the mixture was stirred at rt for 3 h. Then the mixture was poured into water (10 mL). The appeared solid was filtered and purified by flash chromatography to afford 5- (3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)nicotinamide as a white solid (20 mg, 13%). LRMS (M+H+) m/z calculated 527.2, found 527.2. ¾ NMR (DMSO-d6, 300 MHz) δ 13.08 (s, IH), 9.27 (t, IH), 8.86 (d, IH), 8.63 (d, IH), 8.07-8.06 (m, IH), 7.90 (d, IH), 7.72 (dd, IH), 7.54-7.51 (m, 2H), 7.46-7.41 (m, 2H), 7.35-7.26 (m, 2H), 6.88 (d, IH), 6.39 (d, IH), 6.22 (t, IH), 5.21 (s, 2H), 4.78 (d, 2H), 4.01 (s, 2H).
Example 63: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2,5-difluoro-4-((2- oxopyridin-1 yl)methyl)benzyl)nicotinamide
Figure imgf000139_0001
N ((5 chioro 1 H indazoi 3 y|)methyl) 5 (2'5 difiuoro 4 ^2 oxopyrjdin <| (2H) y|)methyl)benzy|)njcotinamjde
Figure imgf000139_0002
To a solution of methyl 2,5-difluoro-4-methylbenzoate (500 mg, 2.69 mmol, 1.0 eq) in CCL (6 mL) were added NBS (527 mg, 2.96 mmol, 1.1 eq), AIBN (13 mg, 0.08 mmol). The mixture was stirred at 85 °C overnight. Then the mixture was evaporated to dryness, and the residue was purified via silica column (PE/EtOAc=100/l) to solid (190 mg, 27%).
Figure imgf000139_0003
To a solution of methyl 4-(bromomethyl)-2,5-difluorobenzoate (500 mg, 2.0 mmol, 1.0 eq), pyridin-2(lH)- one (232 mg, 2.44 mmol) in CH3CN (10 mL) was added K2CO3 (841 mg, 6.1 mmol, 3.0 eq). The mixture was stirred at 70 °C for 2 h. The mixture was evaporated to dryness, the residue was purified via silica column (PE/EtOAc = 1/1) to afford methyl 2,5-difluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate as a white solid (528 mg, 78%).
Figure imgf000140_0001
To a solution of methyl 2,5-difluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate (400 mg, 1.43 mmol, 1.0 eq) in THF (30 mL) was added LiBFL (157 mg, 7.17 mmol, 5.0 eq). The mixture was stirred at 70 °C for 2 h. After cooling to rt, 20 mL of ¾0 was added into the mixture, and the mixture was extracted with EA (3x30 mL). The organic extracts were concentrated to afford l -(2,5-difluoro-4-(hydroxymethyl)benzyl)pyridin- 2(lH)-one as a yellow solid (300 mg, 83%).
Figure imgf000140_0002
To a solution of l-(2,5-difluoro-4-(hydroxymethyl)benzyl)pyridin-2(lH)-one (300 mg, 1.19 mmol, 1.0 eq) in THF (5 mL) was added PBr3 (355 mg, 1.31 mmol, 1.1 eq) at 0 °C. The mixture was stirred at 0 °C for 2 h. 10 mL of H2O was added into the mixture, and the mixture was extracted with EA (3x15 mL). The organic extracts were concentrated to afford l-(4-(bromomethyl)-2,5-difluorobenzyl)pyridin-2(lH)-one as a yellow solid (350 mg, 94%).
Figure imgf000140_0003
The mixture of (5-(ethoxycarbonyl)pyridin-3-yl)boronic acid (195 mg, 1.0 mmol, 1.0 eq), l-(4- (bromomethyl)-2,5-difluorobenzyl)pyridin-2(lH)-one (314 mg, 1.0 mmol, 1.0 eq), Pd(Pli3P)4 (115 mg, 0.1 eq) and Na2CC>3 (318 mg, 3.0 mmol, 3.0 eq) in dioxane (10 mL) and H2O (1.0 mL) was degassed with N2 and stirred at 85 °C overnight. After cooling to rt, the mixture was concentrated. The residue was diluted with EtOAc (100 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via silica column (PE/EtOAc = 1/4) to afford ethyl 5-(2,5-difluoro-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinate as a yellow solid (120 mg, 31%>).
Figure imgf000141_0001
To a solution of ethyl 5-(2,5-difluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate (120 mg, 0.31 mmol, 1.0 eq) in THF (6 mL) and H20 (2 mL) was added LiOH.H20 (131 mg, 3.1 mmol, 10.0 eq). The mixture was stirred at rt overnight. The mixture was evaporated to dryness to afford 5-(2,5-difluoro-4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid as a yellow solid (111 mg, quant), which was used in the next step
Figure imgf000141_0002
A mixture of 5-(2,5-difluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (111 mg, 0.31 mmol, 1.0 eq), (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (83 mg, 0.31 mmol, 1.0 eq), HATU (177 mg, 0.47 mmol, 1.5 eq) and TEA (94 mg, 0.93 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt overnight. The mixture was purified directly by flash chromatography to afford N-((5-chloro-l -(tetrahydro- 2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(2,5-difluoro-4-((2-oxopyridin-l (2H)- yl)methyl)b
Figure imgf000141_0003
A mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(2,5-difluoro-4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (85 mg, 0.17 mmol, 1.0 eq) in HCl/MeOH (25% w/w, 6 mL) was stirred at rt for 2 h. The mixture was purified directly by flash chromatography to afford N-((5- chloro- 1 H-indazol-3 -yl)methyl)-5-(2,5-difluoro-4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinamide as a white solid (25 mg, 35%). LRMS (M+H+) m/z calculated 520.13, found 520.1. ¾ NMR (CD3OD, 400 MHz) δ 9.20 (s, 1H), 8.95 (s, 1H), 8.91 (s, 1H), 7.91 (s, 1H), 7.85 (d, 1H), 7.67 (t, 1H), 7.52 (d, 1H), 7.38 (d, 1H), 7.28 (t, 1H), 7.11 (t, 1H), 6.64 (d, 1H), 6.54 (t, 1H), 5.25 (s, 2H), 4.96 (s, 2H), 4.32 (s, 2H).
Example 64: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-l-(2,5-difluoro-4-((2- oxopyridin-l(2H)- yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
Figure imgf000142_0001
dihydropyridine"3"barboxamic|e
Figure imgf000142_0002
To a solution of l-(4-(bromomethyl)-2,5-difluorobenzyl)pyridin-2(lH)-one (200 mg, 0.63 mmol, 1.0 eq), methyl 6-oxo-l,6-dihydropyridine-3-carboxylate (97 mg, 0.63 mmol) in DMF (5 mL) was added K2CO3 (264 mg, 1.91 mmol, 3.0 eq). The mixture was stirred at RT overnight. The mixture was evaporated to dryness, the residue was purified via silica column (PE/EtOAc = 1/1) to afford methyl l-(2,5-difluoro-4-((2-oxopyridin- l(2H)-yl)methyl)benzyl) 0 mg, 62%).
Figure imgf000142_0003
To a solution of methyl l-(2,5-difluoro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine- 3-carboxylate (170 mg, 0.44 mmol, 1.0 eq) in THF (6 mL) and H20 (2 mL) was added LiOH.H20 (184 mg, 4.4 mmol, 10.0 eq). The mixture was stirred at rt overnight. The mixture was evaporated to dryness to afford 1 -(2,5-difluoro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)-6-oxo-l ,6-dihydropyridine-3-carboxylic acid as a white sol
Figure imgf000142_0004
A mixture of l-(2,5-difluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3- carboxylic acid (164 mg, 0.44 mmol, 1.0 eq), (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methanamine (129 mg, 0.48 mmol, 1.0 eq), HATU (251 mg, 0.66 mmol, 1.5 eq) and TEA (133 mg, 1.32 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt overnight. The mixture was purified directly by flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-l -(2,5- difluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide as a colorless oil (70 mg, 26%)
Figure imgf000143_0001
A mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-l-(2,5-difluoro-4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide (70 mg, 0.11 mmol, 1.0 eq) in HCl/MeOH (25% w/w, 6 mL) was stirred at rt for 2 h. The mixture was purified directly by flash
chromatography to afford N-((5-chloro-lH-indazol-3-yl)methyl)-l-(2,5-difluoro-4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide as a white solid (15 mg, 25%). LRMS (M+H+) m/z calculated 536.12, found 536.1. ¾ NMR (CD3OD, 400 MHz) δ 8.31 (s, 1H), 7.84-7.86 (m, 2H), 7.78 (s, 1H), 7.67 (t, 1H), 7.40 (d, 1H), 7.28 (m, 1H), 7.00 (t, 2H), 6.67 (d, 1H), 6.61 (t, 1H), 6.42 (d, 1H), 5.20 (s, 2H), 5.09 (s, 2H), 4.77 (s, 2H).
Example 65: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2,5-dichloro-4-((2- oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000143_0002
A mixture of l,4-dichloro-2,5-dimethylbenzene (5 g, 0.03 mol, 1.0 eq), NBS (10.5 g, 0.06 mol) and AIBN (50 mg, 0.3 mmol) in CCL (200 mL) was heated at 85 °C under N2 for 2 h. The solvents were evaporated, diluted with water (200 mL) and extracted with DCM (100 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via flash chromatography (PE) to afford l,4-bis(bromomethyl)-2,5-dichlorobenzene as a white solid (850 mg, 10%).
Figure imgf000144_0001
A mixture of l ,4-bis(bromomethyl)-2,5-dichlorobenzene (300 mg, 0.91 mmol), pyridin-2(lH)-one (86 mg,
0.91 mmol) and K2C03 (152 mg, 1.0 mmol) in CH3CN (5 mL) was heated at 80 °C for 4 h. The mixture was diluted with water (100 mL) and extracted with EA (20 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via flash chromatography to afford l-(4-(bromomethyl)-2,5-dichlorobenzyl)pyridin-2(lH)-one as a white solid (100 mg, 30%).
Figure imgf000144_0002
A mixture of l-(4-(bromomethyl)-2,5-dichlorobenzyl)pyridin-2(lH)-one (100 mg, 0.29 mmol), (5- (ethoxycarbonyl)pyridin-3-yl)boronic acid (65 mg, 0.30 mmol), Pd(PPli3)4 (35 mg, 0.03 mmol) and Na2CC>3 (92 mg, 0.87 mmol) in 1,4-dioxane (10 mL) and H2O (1 mL) was heated at 85 °C for overnight. The mixture was diluted with water (100 mL) and extracted with EA (20 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via flash chromatography to afford ethyl 5-(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate as a colorness oil (50 mg, 27%).
Figure imgf000144_0003
A solution of ethyl 5-(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate (50 mg, 0.123 mmol) and LiOH (50 mg, 1.2 mmol) in THF (5 mL) and H2O (1 mL) was stirred at rt overnight, then concentrated to afford 5-(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid as a white solid (40 mg, 90%), which was used
Figure imgf000144_0004
A mixture of 5-(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (180 mg, 0.47 mmol), (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (130 mg, 0.47 mmol), HATU (265 mg, 0.71 mmol) and TEA (0.2 mL, 1.4 mmol) in DMF (5 mL) was stirred at rt for 2 h. The mixture was concentrated and purified via flash chromatography to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)-5-(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (50 mg, 46%)
Figure imgf000145_0001
A solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(2,5-dichloro-4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (50 mg, 0.06 mmol) in HCl/MeOH (5 mL) was stirred at rt for overnight. The mixture was concentrated and purified by prep-HPLC to afford N-((5-chloro-lH-indazol-3- yl)methyl)-5-(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (30 mg, 80%). LRMS (M+H+) m/z calculated 552, found 552.1. ¾ NMR (CD3OD, 400 MHz) δ 9.22 (s, 1 H), 8.94 (s, 1 H), 8.89 (s, 1 H), 7.94 (s, 1 H), 7.93-7.91 (d, 1 H), 7.80-7.76 (m, 1 H ), 7.68 (s, 1 H), 7.55-7.53 (d, 1 H), 7.42-7.39 (m, 1 H ), 7.20 (s, 1 H), 6.79-6.77 (d, 1 H), 6.70-6.66 (m, 1 H ), 5.37 (s, 2 H), 4.98 (s, 2 H), 4.43 (s, 2 H).
Example 66: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-l-(2,5-dichloro-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
Figure imgf000145_0002
N ((5 chioro 1 H indazoi 3 yUmethyl) 1 (2'5 dichioro 4 ,,2 oxopyridin <| ,2H\ yKmethyKbenzyK 6 oxo e
dihydropyridine"3"bWboxam e
Figure imgf000145_0003
A mixture of l-(4-(bromomethyl)-2,5-dichlorobenzyl)pyridin-2(lH)-one (150 mg, 0.43 mmol), K2CO3 (180 mg, 1.30 mmol) and methyl 6-oxo-l,6-dihydropyridine-3-carboxylate (73 mg, 0.48 mmol) in DMF (5 mL) was stirred at rt for overnight. The mixture was diluted with water (100 mL) and extracted with EA (50 mL X
3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via flash chromatography to afford methyl l-(2,5-dichloro-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-2-oxo-l,2-dihydropyridine-4-carboxylate as a colorless oil (170 mg, 90%).
Figure imgf000146_0001
A solution of methyl l-(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-2-oxo-l,2-dihydropyridine-4- carboxylate (180 mg, 0.43 mmol) and LiOH (180 mg, 4.3 mmol) in THF (10 mL) and ¾0 (1 mL) was stirred at rt for overnight, then concentrated to afford l-(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-2- oxo-l,2-dihydropyridine-4-carboxylic acid as a yellow solid (150 mg, 86%), which was used directly in the next step without further purification.
Figure imgf000146_0002
A mixture of l-(2,5-dichloro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)-2-oxo-l,2-dihydropyridine-4- carboxylic acid (180 mg, 0.43 mmol), (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (130 mg, 0.47 mmol), HATU (250 mg, 0.71 mmol) and TEA (130 mg, 1.40 mmol) in DMF (5 mL) was stirred at rt for 2 h. The mixture was diluted with water (100 mL) and extracted with EA (50 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl) -(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3- carboxamide as a white solid (60 mg, 40%).
Figure imgf000146_0003
A solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-l-(2,5-dichloro-4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l ,6-dihydropyridine-3-carboxamide (60 mg, 0.09 mmol) in HCl/MeOH (5 mL) was stirred at rt for overnight. The mixture was concentrated and purified by prep-HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-l -(2,5-dichloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6- oxo-1, 6-dihydropyridine-3-carboxamide as a white solid (30 mg, 60%). LRMS (M+H+) m/z calculated 568, found 568.1. ¾ NMR (CD3OD, 400 MHz) δ 8.20 (s, 1 H), 7.85-7.82 (d, 1 H), 7.69(s, 1 H), 7.54-7.52 (d, 1 H), 7.44-7.43 (m, 1 H), 7.33-7.31(d, 1 H), 7.20-7.18 (d, 1 H), 7.01 (s, 1 H), 6.97 (s, 1 H), 6.47-6.44 (m, 2 H), 6.31-6.29 (m, 1 H), 5.11-5.09 (d, 4 H), 4.77 (s, 2 H).
Example 67: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(5-chloro-2-fluoro-4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)nicotinamide
Figure imgf000147_0001
N ((5 chioro 1 H indazoi 3 yi methyl) 5 (5 chioro 2 fiuoro 4 ^2 oxopyHdin 1 (2H) yljmethyljbenzyijnicotinamicie
Figure imgf000147_0002
To a solution of methyl 4-(bromomethyl)-5-chloro-2-fluorobenzoate (400 mg, 1.42 mmol, 1.2 eq) in CH3CN were added pyridin-2(lH)-one (162 mg, 1.70 mmol, 1.2 eq), K2CO3 (393 mg, 2.84 mmol), and the mixture was stirred at 60 °C for 3 h. The reaction mixture was concentrated and purified by flash chromatography to afford methyl 5-chloro-2- e solid (380 mg, 96%).
Figure imgf000147_0003
To a solution of methyl 5-chloro-2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate (400 mg, 1.43 mmol, 1 eq) in THF was added L1BH4 (624 mg, 28.6 mmol, 20 eq) under N2. The mixture was stirred at 70 °C for 5 h. The reaction mixture was concentrated and purified by flash chromatography to afford l-(2-chloro-5-fluoro-4- (hydroxymethyl)benzyl)
Figure imgf000147_0004
To a solution of l-(2-chloro-5-fluoro-4-(hydroxymethyl)benzyl)pyridin-2(lH)-one (300 mg, 1.12 mmol, 1 eq) in DCM was added PBr3, and the mixture was stirred at rt for 3 h. The reaction mixture was poured into ice- water and extracted with DCM; the organic phase was washed with NaHCC (aq), concentrated and purified by p-TLC to afford l -(4-(bromomethyl)-2-chloro-5-fluorobenzyl)pyridin-2(lH)-one as a white solid (170 mg, 45 %).
Figure imgf000148_0001
To a solution of l-(4-(bromomethyl)-2-chloro-5-fluorobenzyl)pyridin-2(lH)-one (170 mg, 0.513 mmol) in Dioxane /H2O were added ethyl 5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)nicotinate (142 mg, 0.513 mmol, 1 eq), Na2CC>3 (163 mg, 1.539 mmol, 3 eq) and Pd(PPli3)4 under N2. The mixture was stirred at 80 °C for 5 h. The reaction mixture was concentrated and purified by prep-TLC to afford ethyl 5-(5-chloro-2-fluoro- 4-((2-oxopyridin- -yl)methyl)benzyl)nicotinate as a yellow oil (100 mg, 49 %).
Figure imgf000148_0002
To a solution of ethyl 5-(5-chloro-2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate (100 mg, 0.25 mmol, leq) in THF/H2O was added LiOH (60 mg, 2.50 mmol, 10 eq). The mixture was stirred rt overnight. It
Figure imgf000148_0003
To a solution of 5-(5-chloro-2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (100 mg, 0.27 mmol, 1 eq) in DMF was added (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (100 mg, 0.38 mmol, 1.4 eq), TEA (82 mg, 0.81 mmol, 3 eq), HATU (144 mg, 0.38 mmol). The mixture was stirred at rt for 5 h. The reaction mixture was concentrated and purified by prep-TLC to afford N-((5-chloro-l-
(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-5-(5-chloro-2-fluoro-4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)nicotinamide (100 mg). It (100 mg, 0.16 mmol) was dissolved in MeOH (HC1) and stirred for 2h, concentrated and purified by prep-HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-(5-chloro- 2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (20 mg, 23 %). LRMS (M+H+) m/z calculated 536.1, found 536.1. ¾ NMR (CD3OD, 400 MHz) δ 8.84 (s, 1 H), 8.57 (s, 1 H), 8.08 (s, 1 H), 7.85 (s, 1 H), 7.66-7.32 (m , 5 H), 6.78 (d, 1H), 6.58 (d, 1 H), 5.22 (s, 2 H), 4.07 (s, 2 H), 3.33 (s, 2 H).
Example 68: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-l -(5-chloro-2-fluoro-4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)-6-oxo-l,6-di
Figure imgf000149_0001
N ((5 chioro 1 H indazoi 3 ykmethyl) 1 (5 chioro 2 fluoro 4 ,,2 oxopyrjdin <| ,2H) yhmethyhbenzyh 6 oxo 1 '6
dih dropyridine"3"carboxamide
Figure imgf000149_0002
To a solution of l-(4-(bromomethyl)-2-chloro-5-fluorobenzyl)pyridin-2(lH)-one (300 mg, 0.907 mmol, 1 eq) in DMF was added methyl 6-oxo-l,6-dihydropyridine-3-carboxylate (208 mg, 1.36 mmol, 1.5 eq) and K2CO3. The mixture was stirred at rt overnight, then poured into water and extracted with EtOAc. The organic layers were concentrated, purified by prep-TLC (DCM/MeOH = 10/1) to afford methyl l-(5-chloro-2-fluoro-4-((2- oxopyridin-l(2H)-yl)me mg, 27%).
Figure imgf000149_0003
To a solution of methyl l -(5-chloro-2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6- dihydropyridine-3-carboxylate (100 mg, 0.248 mmol, 1 eq) in THF/H2O was added LiOH (60 mg, 2.48 mmol, 10 eq). The mixture was stirred at rt overnight, then concentrated. The residue was used for the next step without further purification.
Figure imgf000150_0001
To a solution of l-(5-chloro-2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3- carboxylic acid (100 mg, 0.257 mmol, 1 eq) in DMF were added (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methanamine (103 mg, 0.257 mmol, 1 eq), TEA (52 mg, 0.514 mmol, 2 eq), HATU ( 147 mg, 0.386 mmol, 1.5 eq). The mixture was stirred at rt overnight, poured into water and extracted with EtOAc. The organics was concentrated, purified by prep-TLC to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)- lH-indazol-3-yl)methyl)-l-(5-chloro-2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6- dihydropyridine-3-carboxamide (100 mg, 61 %). It was dissolved in MeOH (HC1); the mixture was stirred at rt overnight, concentrated and purified by prep-HPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-l-(5- chloro-2-fluoro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)-6-oxo-l ,6-dihydropyridine-3-carboxamide as a white solid (10 mg, 12%). LRMS (M+H+) m/z calculated 552.1, found 552.1. ¾ NMR (d6-DMSO, 400 MHz) δ 13.17-13.06 (m, 1 H), 8.96-8.92 (m, 1 H), 8.50 (s, 1 H), 7.96-7.89 (m, 2 H), 7.75-7.71 (m , 1 H), 7.54-7.48 (m, 2H), 7.38-7.32 (m, 2 H), 6.68 (d, 1 H), 6.46-6.27 (m, 3 H), 5.17-4.74 (m, 6 H). Example 69: Preparation of N-(4-aminobenzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide
Figure imgf000150_0002
N (4 amjnobenzyi) 5 ,4 ((2 oxopyrjdin i (2Hj
ljmethyljbenzyijnicotinamide
Figure imgf000150_0003
A mixture of methyl ethyl 5-bromonicotinate (5.0 g, 21.73 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'- bi(l,3,2-dioxaborolane) (5.5 g, 21.73 mmol, 1.0 eq), Pd(dppf)Cl2 (790 mg, 1.08 mmol, 5%) and KOAc (6.4 g, 65.21 mmol, 3.0 eq) in dioxane (180 mL) was degassed with N2 and stirred at 85 °C for 3 h, cooled, l-(4- (bromomethyl)benzyl)pyridin-2(lH)-one (6.0 g, 21.73 mmol, 1.0 eq), Pd(dppf)Cl2 (790 mg, 1.08 mmol, 5%) and an aqueous solution of Na2C03 (6.9 g, 65.21 mmol, 3.0 eq) in 30 mL of water were added. The mixture was degassed and stirred at 95 °C under N2 overnight. Cooled and filtered through celite, concentrated, the residue was dissolved in DCM (150 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via silica gel column chromatography (5% MeOH in DCM) then purified by flash chromatography to afford ethyl 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate as a yellow solid (4.17 g, 55.1%).
Figure imgf000151_0001
To a stirred solution of methyl ethyl 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinate (2.5 g, 7.18 mmol, 1.0 eq) in MeOH (15 mL) was added an aqueous solution of NaOH (570 mg, 14.36 mmol, 2.0 eq) in water (10 mL). The mixture was stirred at ambient temperature for 1.5 h, concentrated, the residue was acidified with 2 N HCl till pH value was 1 , concentrated in vacuo, the residue was dissolved in 10% MeOH in DCM, filtered, the filtrate was concentrated to dryness to afford 5-(4-((2-oxopyridin-l(2H)- yl)methyl)b
Figure imgf000151_0002
To a stirred mixture of 5-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)nicotinic acid (100 mg, 0.31 mmol, 1.0 eq) and HATU (178 mg, 0.46 mmol, 1.5 eq) in DMF (4 mL) was added 4-(aminomethyl)aniline (90 mg, 0.73 mmol, 2.3 eq) at 10 °C, the mixture was stirred for 20 min, TEA (0.5 mL) was added. The reaction was stirred at ambient temperature overnight, poured into water, extracted with DCM (15 mL X 3). The combined organic layer was washed with brine, dried over anhydrous Na2S04, concentrated, and purified via prep- HPLC to afford N-(4-aminobenzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (30 mg, 22%). LRMS (M+H+) m/z calculated 425.2, found 425.2. ¾ NMR (DMSO-d6, 400 MHz) δ
9.00 (t, 1 H), 8.84 (s, 1 H), 8.60 (s, 1 H), 8.02 (s, 1 H), 7.75 (dd, 1 H), 7.40 (t, 1 H), 7.25-7.20 (m, 4 H) , 6.96 (d, 2 H) , 6.49 (d, 2 H) , 6.39 (d, 1 H) , 6.20 (t, 1 H), 5.04 (s, 2 H), 4.97 (s, 2 H), 4.28 (d, 2 H), 3.98 (s, 2 H).
Example 70: Preparation of N-((5-chlorobenzo[b]thiophen-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000152_0001
The mixture of 3-(bromomethyl)-5-chlorobenzo[b]thiophene (500 mg, 1.9 mmol, 1.0 eq), isoindo line- 1,3- dione (335 mg, 2.28 mmol, 1.2 eq) and K2C03 (498 mg, 3.61 mmol, 3.0 eq) in CH3CN (20 mL) was stirred at 80 °C overnight. Then the mixture was cooled to rt and filtered. The filtrate was concentrated. The residue was diluted with water (50 mL), extracted with EtOAc (30 mL X 3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was dried to afford 2-((5- chlorobenzo[b]thiophen-3-yl)methyl)isoindoline-l,3-dione as a yellow solid (600 mg, 96%).
Figure imgf000152_0002
To a suspension of 2-((5-chlorobenzo[b]thiophen-3-yl)methyl)isoindoline-l,3-dione (600 mg, 1.83 mmol, 1.0 eq) in DCM/THF (3 mL/2 mL) was added N2H4.H20 (458 mg, 9.16 mmol, 5.0 eq). The mixture was stirred at rt overnight. Then the mixture was concentrated. The residue was purified on silica gel column (PE/EtOAc = 2/1) to give (5-chlorobenzo[b]thiophen-3-yl)methanamine as a white solid (280 mg, 77%).
Figure imgf000152_0003
A mixture of (5-chlorobenzo[b]thiophen-3-yl)methanamine (60 mg, 0.3 mmol, 1.0 eq), 5-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)nicotinic acid (97 mg, 0.3 mmol, 1.0 eq), HATU (171 mg, 0.45 mmol, 1.5 eq) and DIEA (116 mg, 0.9 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt overnight. The mixture was poured into water (50 mL). The precipitate was collected and purified by pre-HPLC to afford N-((5- chlorobenzo[b]thiophen-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (6.2 mg, 4%). LRMS (M+H+) m/z calculated 500.12, found 500.1. ¾ NMR (CD3OD, 400 MHz) δ 8.83 (s, 1H), 8.57 (s, 1H), 8.08 (s, 1H), 7.97 (s, 1H), 7.88 (d, 1H), 7.69 (d, 1H), 7.62 (s, 1H), 7.53 (td, 1H), 7.37 (d, 1H), 7.28-7.23 (m, 4H), 6.57 (d, 1H), 6.39 (d, 1H), 5.17 (s, 2H), 4.79 (s, 2H), 4.07 (s, 2H).
Example 71: Preparation of N-((6-aminopyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000153_0001
To a solution of 6-aminonicotinonitrile (1.0 g, 8.4 mmol, 1.0 eq) in DCM (10 mL) was added Et3N (1.7 g, 16.8 mmol, 2.0 eq), DMAP (102 mg, 0.84 mmol, 0.1 eq) and Boc20 (3.7 g, 16.8 mmol, 2.0 eq) at rt. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford tert-butyl (5-cyanopyridin-2-yl)carbamate as a white solid (1.7 g, 92%).
Figure imgf000153_0002
To a solution of tert-butyl (5-cyanopyridin-2-yl)carbamate (500 mg, 2.28 mmol, 1.0 eq) in Nl¾/MeOH (10 mL) was added Raney Ni (about 100 mg). The mixture was hydrogenated at rt overnight. The mixture was filtered and the filtrate was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford tert-butyl (5-(aminomethyl)pyridin-2-yl)carbamate as a white solid (240 mg, 47%).
Figure imgf000153_0003
To a solution of tert-butyl (5-(aminomethyl)pyridin-2-yl)carbamate (240 mg, 1.08 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/l,4-dioxane (2 mL). The mixture was stirred at rt for 2 h. The mixture was concentrated to dryness under reduced pressure to afford 5-(aminomethyl)pyridin-2-amine as a white solid (300 mg crude, quant).
Figure imgf000154_0001
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (100 mg, 0.31 mmol, 1.0 eq) in DMF (2 mL) was added DIPEA (200 mg, 1.55 mmol, 5.0 eq), HATU (141 mg, 0.37 mmol, 1.2 eq) and 5- (aminomethyl)pyridin-2-amine (300 mg crude, 1.08 mmol, 3.5 eq). The mixture was stirred at rt overnight. The mixture was added water (10 mL), extracted with EA (10 mL x 3), the combined organic layer was washed with brine (10 mL). The combined organic layers were dried over anhydrous Na2S04 and
concentrated. The residue was purified via flash chromatography chromatography to afford N-((6- aminopyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (38 mg, 29%). LRMS (M+H+) m/z calculated 426.2, found 426.2. ¾ NMR (DMSO-< 6, 400 MHz) δ 9.02 (t, 1 H), 8.83 (s, 1 H), 8.61 (s, 1 H), 8.01 (s, 1 H), 7.85 (s, 1 H), 7.75 (d, 1 H), 7.40 (td, 1 H), 7.33 (d, 1 H), 7.30-7.20 (m, 4 H), 6.45-6.35 (m, 2 H), 6.21 (t, 1 H), 5.87 (brs, 2 H), 5.04 (s, 2 H), 4.25 (d, 2 H), 3.98 (s, 2 H).
Example 72: Preparation of N-(4-(aminomethyl)benzyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000154_0002
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (160 mg, 0.5 mmol, 1.0 eq.), tert- butyl 4-(aminomethyl)benzylcarbamate (142 mg, 0.6 mmol, 1.2 eq.), HATU (250 mg, 1.3 mmol, 1.3 eq.) and DIPEA (0.8 mL) in DMF (5 mL) was stirred at 50 °C overnight. The mixture was poured into water (20 mL) and filtered. The solid was collected to afford tert-butyl 4-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)benzylcarbamate as a yellow solid (230 mg, 85.5%).
Figure imgf000155_0001
A solution of tert-butyl 4-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)benzylcarbamate (101 mg, 0.188 mmol, 1 eq.) in DCM (2 mL) and HCl/dioxane (5 N, 2 mL) was stirred at rt for 2 h, then concentrated. The residue was triturated with
DCM/MeOH = 10/1, v/v) to afford N-(4-(aminomethyl)benzyl)-5-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)nicotinamide hydrochloride as a brown solid (80 mg, 89.9%). LRMS (M+H+) m/z calculated 439.2, found 439.2. ¾ NMR (CD3OD, 400 MHz): δ 9.17 (s, 1 H), 7.84 (d, 1 H), 7.65 (t-d, 1 H),7.50-7.45 (m, 4 H), 7.35 (s, 4 H), 6.67 (d, 1 H), 6.54 (t, 1 H), 5.25 (s, 2 H), 4.65 (s, 2 H), 4.32 (s, 2 H), 4.13 (s, 2 H).
Example 73: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000155_0002
The mixture of 4,6-dimethylpyridin-2-amine (1 g, 8.2 mmol, 1.0 eq) and NBS (1.46 g, 8.2 mmol, 1.0 eq) in CH3CN (30 mL) was stirred at rt overnight. Then the mixture was concentrated, and the residue was purified on silica gel column (PE/EtOAc = 5/1) to afford 5-bromo-4,6-dimethylpyridin-2-amine as a yellow solid (800 mg, 48%).
Figure imgf000155_0003
The mixture of 5-bromo-4,6-dimethylpyridin-2-amine (800 mg, 3.98 mmol, 1.0 eq) and CuCN (425 mg, 4.78 mmol, 1.2 eq) in NMP (10 mL) was stirred at 200 °C for 1 h with microwave. Then the mixture was cooled to rt and directly purified by flash chromatography to give 6-amino-2,4-dimethylnicotinonitrile as a brown oil (585 mg, crude, include NMP).
Figure imgf000156_0001
A mixture of 6-amino-2,4-dimethylnicotinonitrile (585 mg, 3.98 mmol, 1.0 eq), B0C2O (1.74 g, 7.96 mmol, 2.0 eq), TEA (1.2 g, 12mmol, 3.0 eq) and DMAP (49 mg, 0.4 mmol, 0.1 eq) in 1,4-dioxane (10 mL) was stirred at rt overnight. Then the mixture was purified on silica gel column (PE/EtOAc = 5/1) to afford tert- butyl (5-cyano-4,6-dimethylpyridin-2-yl)carbamate as a white solid 720 mg, 73%).
Figure imgf000156_0002
The solutuion of tert-butyl (5-cyano-4,6-dimethylpyridin-2-yl)carbamate (720 mg, 2.9 mmol, 1.0 eq) and Raney Ni (20 mg) in MeOH (15 mL) was stirred at rt under ¾ (1 atm) overnight. Then the mixture was filtered and the filtrate was concentrated to give tert-butyl (5-(aminomethyl)-4,6-dimethylpyridin-2- yl)carbamate as a colorless oil (720 mg, 99%).
Figure imgf000156_0003
The mixture of tert-butyl (5-(aminomethyl)-4,6-dimethylpyridin-2-yl)carbamate (720 mg, 2.9 mmol, 1.0 eq) in 2 N HCI/dioxane (15 mL) and MeOH (3 mL) was stirred at rt overnight. Then the mixture was filtered to give 5-(
Figure imgf000156_0004
A mixture of 5-(aminomethyl)-4,6-dimethylpyridin-2-amine (117 mg, 0.63 mmol, 1.0 eq), 5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (200 mg, 0.63 mmol, 1.0 eq), HATU (479 mg, 1.26 mmol, 2.0 eq) and DIEA (406 mg, 3.15 mmol, 5.0 eq) in DMF (5 mL) was stirred at rt for 2 h. The mixture was poured into water (50 mL), extracted with DCM (30 mLx3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified by flash chromatography to afford N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a white solid (91.2 mg, 32%). LRMS (M+H+) m/z calculated 454.2, found 454.2. 'H NMR (DMSO-d6, 400 MHz) δ 8.80 (d, 1H), 8.58 (d, 1H), 7.99 (s, 1H), 7.75 (dd, 1H), 7.40 (td, 1H), 7.22 (s, 4H), 6.39 (d, 1H), 6.23-6.21 (m, 2H), 5.99 (brs, 2H), 5.04 (s, 2H), 4.32 (d, 2H), 3.98 (s, 2H), 2.33 (s, 3H), 2.20 (s, 3H).
Example 74: Preparation of N-((6-chloro-lH-indol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000157_0001
N ((6 chioro 1 H indoi 3 yl)methy|) 5 (4 ((2 oxopyHdin < (2H) y^methyl^enzyi^icotinamide
Figure imgf000157_0002
To a solution of 6-chloro-lH-indole-3-carbaldehyde (1.0 g, 5.66 mmol, 1.0 eq) in NLh/MeOH (10 mL) was added NH2OH.HCI (502 mg, 7.2 mmol, 1.3 eq) at rt. The mixture was stirred at rt for 2 h. The mixture was concentrated to dryness under reduced pressure to afford 6-chloro-lH-indole-3-carbaldehyde oxime as a yellow solid (1.6 g crude, quant).
Figure imgf000157_0003
To a solution of 6-chloro-lH-indole-3-carbaldehyde oxime (360 mg, 1.84 mmol, 1.0 eq) in NL /MeOH (5 mL) was added Raney Ni (about 36 mg). The suspension was hydrogenated at rt overnight. The mixture was filtered. The filtered cake was washed with MeOH (10 mL). The filtrate was concentrated under reduced pressure to afford (6- e, quant).
Figure imgf000157_0004
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (150 mg, 0.47 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (182 mg, 1.41 mmol, 3.0 eq) and HATU (213 mg, 0.56 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (6-chloro-lH-indol-3-yl)methanamine (93 mg, 0.52 mmol, 1.1 eq) was then added and the mixture was stirred at rt for 2 h under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford 110 mg of product, which was further purified via Prep-HPLC to afford N-((6-chloro-lH-indol-3- yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a yellow solid (50 mg, 22%). LRMS (M+H+) m/z caculated 483.1, found 483.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.08 (brs, 1 H), 9.00 (t, 1 H), 8.83 (s, 1 H), 8.59 (s, 1 H), 8.02 (s, 1 H), 7.75 (d, 1 H), 7.62 (d, 1 H), 7.45-7.35 (m, 2 H), 7.34 (s, 1 H), 7.25- 7.15 (m, 4 H), 7.00 (d, 1 H), 6.39 (d, 1 H), 6.21 (t, 1 H), 5.04 (s, 2 H), 4.59 (d, 2 H), 3.98 (s, 2 H).
Example 75: Preparation of N-((5-chloro-lH-indol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000158_0001
N ((5 chioro 1 H indoi 3 yljmethyl) 5 (4 ((2 oxopyndin <\ βΗ) yljmethyljbenzyijnicotinamide
Figure imgf000158_0002
To a solution of 5-chloro-lH-indole-3-carbaldehyde (1.0 g, 5.66 mmol, 1.0 eq) in NELVMeOH (10 mL) was added NH2OH.HCI (502 mg, 7.2 mmol, 1.3 eq) at rt. The mixture was stirred at rt for 2 h. The mixture was concentrated to dryness under reduced pressure. The crude product was purified via flash chromatography to afford 5-chloro-lH-indole-3-carbal 2%).
Figure imgf000158_0003
To a solution of 5-chloro-lH-indole-3-carbaldehyde oxime (600 mg, 3.08 mmol, 1.0 eq) in AcOH (10 mL) was added Zn (1.0 g, 15.4 mmol). The suspension was heated to 30 °C overnight. The mixture was concentrated to dryness under reduced pressure. The residue was added water (10 mL), saturated NaHCCb (20 mL), extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated to afford (5-chloro-lH-indol-3-yl)methanamine as a yellow solid (380 mg crude, 68%).
Figure imgf000159_0001
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (150 mg, 0.47 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (182 mg, 1.41 mmol, 3.0 eq) and HATU (213 mg, 0.56 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-lH-indol-3-yl)methanamine (93 mg, 0.52 mmol, 1.1 eq) was then added and the mixture was stirred at rt for 2 h under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-lH-indol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (40 mg, 18%). LRMS (M+H+) m/z caculated 483.1, found 483.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.14 (brs, 1 H), 9.02 (t, 1 H), 8.83 (d, 1 H), 8.59 (d, 1 H), 8.01 (s, 1 H), 7.74 (d, 1 H), 7.68 (s, 1 H), 7.45- 7.35 (m, 3 H), 7.30-7.15 (m, 4 H), 7.07 (dd, 1 H), 6.39 (d, 1 H), 6.20 (t, 1 H), 5.03 (s, 2 H), 4.56 (d, 2 H), 3.98 (s, 2 H).
Example 76: Preparation of N-((lH-indol-6-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000159_0002
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (91 mg, 0.28 mmol, 1.0 eq.), (1H- indol-6-yl)methanamine (44 mg, 0.30 mmol, 1.07 eq.), HATU (114 mg, 0.3 mmol, 1.07 eq.) and DIPEA (0.5 mL) in DCM (3 mL) was stirred at rt overnight. Filtered and the solid was collected and triturated with water to afford N-((lH-indol-6-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (100 mg, 78.7%). LRMS (M+H+) m/z calculated 449.2, found 449.2. ¾ NMR (CD3OD, 400 MHz) δ 8.85 (s, 1 H), 8.57 (s, 1 H), 8.09 (s, 1 H), 7.69 (d, 1 H), 7.55-7.51 (m, 2 H), 7.39 (t, 1 H), 7.29-7.22 (m, 5 H), 7.03 (d, 1 H), 6.57 (d, 1 H), 6.43-6.37 (m, 2 H), 5.18 (s, 2 H), 4.67 (s, 2 H), 4.08 (s, 2 H).
Example 77: Preparation of N-((3-chloro-lH-indol-6-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000160_0001
The mixture of N-((lH-indol-6-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (56 mg 0.125 mmol, 1.0 eq) in DCM (5 mL) was treated with NCS (16 mg, 0.125 mmol, 1.0 eq). And the reaction was stirred at ambient temperature overnight, concentrated, purified via flash chromatography to afford N-((3 chloro-lH-indol-6-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (15 mg, 25%). LRMS (M+H+) m/z calculated 483.1, found 483.1. lHNMR (CD3OD, 400 MHz) δ 8.73 (s, 1 H), 8.45 (s, 1 H), 7.96 (s, 1 H), 7.56 (d, 1 H), 7.44-7.34 (m, 2 H), 7.26 (s, 1 H), 7.18-7.10 (m, 5 H), 7.01 (d, 1 H), 6.45 (d, 1 H), 6.26 (t, 1 H), 5.05 (s, 2 H), 4.55 (s, 2 H), 3.95 (s, 2 H).
Example 78: Preparation of N-((lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000160_0002
W"((1HΊndazof3"y|Jmethyl)"5"(4"((2"oxopyridin"1(2H)Vl)methyl)benzy|Jnicotinamic|e
Figure imgf000161_0001
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (91 mg, 0.28 mmol, 1.0 eq), (1H- indazol-3-yl)methanamine (44 mg, 0.30 mmol, 1.07 eq), HATU (114 mg, 0.3 mmol, 1.07 eq) and DIPEA (0.5 mL) in DCM (3 mL) was stirred at rt overnight. Filtered and the solid was collected and purified via prep- MPLC to afford N-((lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (40 mg, 31%). LRMS (M+H+) m/z calculated 450.2, found 450.2. ¾ NMR (CD3OD, 400 MHz) δ 8.85 (s, 1 H), 8.56 (s, 1 H), 7.83 (d, 1 H), 7.69 (d, 1 H), 7.55-7.50 (m, 2 H), 7.40 (t, 1 H), 7.26 (dd, 4 H), 7.14 (t, 1 H), 6.58 (d, 1 H) , 6.40 (t, 1 H), 5.18 (s, 2 H), 4.96 (s, 2 H), 4.08 (s, 2 H). Example 79: Preparation of N-((lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000161_0002
To a solution of lH-indole-5-carbonitrile (1.0 g, 7.0 mmol, 1.0 eq) in NLh/MeOH (10 mL) was added Raney
Ni (about 100 mg). The suspension was hydrogenated at rt overnight. The mixture was filtered. The filtered cake was washed with MeOH (10 mL). The filtrate was concentrated under reduced pressure. The residue was purified via flash chromatography to afford (lH-indol-5-yl)methanamine as an off-white solid (330 mg, 32%).
Figure imgf000161_0003
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (60 mg, 0.19 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (74 mg, 0.57 mmol, 3.0 eq) and HATU (87 mg, 0.23 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (lH-indol-5-yl)methanamine (28 mg, 0.19 mmol, 1.0 eq) was then added and the mixture was stirred at rt for 2 h under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((1H- indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a yellow solid (30 mg, 35%). LRMS (M+H+) m/z caculated 449.2, found 449.2. ¾ NMR (DMSO-i e, 400 MHz) δ 11.03 (brs, 1 H), 9.14 (t,
1 H), 8.88 (d, 1 H), 8.61 (d, 1 H), 8.05 (s, 1 H), 7.75 (dd, 1 H), 7.46 (s, 1 H), 7.45-7.35 (m, 1 H), 7.34-7.30 (m,
2 H), 7.26-7.20 (m, 4 H), 7.05 (d, 1 H), 7.45-7.35 (m, 2 H), 6.20 (t, 1 H), 5.03 (s, 2 H), 4.52 (d, 2 H), 3.99 (s, 2 H). Example 80: Preparation of N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000162_0001
To a solution of N-((lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (100 mg, 0.22 mmol, 1.0 eq) in DCM (5 mL) was added NCS (30 mg, 0.22 mmol, 1.0 eq). The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a white solid (30 mg, 28%). LRMS (M-H+) m z caculated 481.1, found 481.2. 'H NMR (DMSO-i e, 400 MHz) δ 11.33 (brs, 1 H), 9.19 (t, 1 H), 8.87 (s, 1 H), 8.62 (d, 1 H), 8.04 (s, 1 H), 7.75 (d, 1 H), 7.50 (d, 1 H), 7.45-7.30 (m, 3 H), 7.28-7.20 (m, 4 H), 7.16 (d, 1 H), 6.39 (d, 1 H), 6.20 (t, 1 H), 5.03 (s, 2 H), 4.56 (d, 2 H), 3.99 (s, 2 H).
Example 81: Preparation of N-((6-amino-2-methylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000163_0001
Figure imgf000163_0002
The solutuion of 6-amino-2-methylnicotinonitrile (200 mg, 1.5 mmol, 1.0 eq) and Raney Ni (50 mg) in MeOH (10 mL) was stirred at rt under ¾ (1 atm) overnight. Then the mixture was filtered and the filtrate was concentrated to give 5-(aminomethyl)-6-methylpyridin-2-amine as a yellow solid (210 mg, quant). The solid was used for the next step without further purification.
Figure imgf000163_0003
A mixture of 5-(aminomethyl)-6-methylpyridin-2-amine (47 mg, 0.34 mmol, 1.1 eq), 5-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinic acid (100 mg, 0.31 mmol, 1.0 eq), HATU (235 mg, 0.62 mmol, 2.0 eq) and DIEA (120 mg, 0.93 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt for 2 h. The mixture was poured into water (50 mL), extracted with EtOAc (30 mLx3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified by flash chromatography to afford N-((6-amino-2-methylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (34 mg, 25%). LRMS (M+H+) m/z calculated 440.2, found 440.2. ¾ NMR (DMSO-d6, 400 MHz) δ 8.95 (t, 1H), 8.84 (s, 1H), 8.61 (s, 1H), 8.00 (s, 1H), 7.75 (dd, 1H), 7.42-7.38 (m, 2H), 7.24-7.20 (m, 4H), 6.40-6.37 (m, 2H), 6.23-6.19 (m, 2H), 5.04 (s, 2H), 4.28 (d, 2H), 3.99 (s, 2H), 2.33 (s, 3H).
Example 82: Preparation of N-(3-chlorobenzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide
Figure imgf000164_0001
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (100 mg, 0.3 mmol, 1.0 eq), (3- chlorophenyl)methanamine (44 mg, 0.31 mmol, 1.0 eq), HATU (236 mg, 0.6 mmol, 2.0 eq) and DIEA (120 mg, 0.9 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt for 2 h. The mixture was poured into water (50 mL), extracted with EtOAc (30 mLx3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified by flash chromatography to afford N-(3- chlorobenzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a yellow solid (74 mg, 54%). LRMS (M+H+) m/z calculated 444.2, found 444.1. ¾ NMR (DMSO-d6, 400 MHz) δ 9.23 (t, 1H), 8.88 (d, 1H), 8.63 (d, 1H), 8.05 (s, 1H), 7.75 (dd, 1H), 7.42-7.21 (m, 9H), 6.38 (d, 2H), 6.21 (t, 1H), 5.04 (s, 2H), 4.47 (d, 2H), 4.01 (s, 2H).
Example 83: Preparation of N-((6-chloro-lH-benzo[d]imidazol-2-yl)methyl)-5-(4-((2-oxopyridin- 1(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000164_0002
N ((6 chioro 1 H benzo^imidazoi 2 yl)methyl) 5 (4 ((2 oxopyndin 1 (2H) y^methyl^enzy^nicotinamide
Figure imgf000164_0003
A mixture of 2-((tert-butoxycarbonyl)amino)acetic acid (2.62 g, 15.0 mmol, 1.0 eq), EDC.HCl (3.44 g, 18.0 mmol, 1.2 eq), HOBt (2.83 g, 21.0 mmol, 1.4 eq) and DCM (150 mL) was stirred at rt for 10 min. Then 4- chlorobenzene-l,2-diamine (2.13 g, 15.0 mmol, 1.0 eq) and DMF(2 mL) were added, and the resulting mixture was stirred at rt overnight. DCM was evaporated and EA (150 mL) was added. The organic phase was washed with brine (30 mL), sat. NH4C1 (30 mL x 2), sat. NaHCOs (30 mL x 2) and finally with brine (30 mL). The resulting organic layer was dried with Na2S04, concentrated to dryness. Then 20 mL of AcOH was added and the mixture was heated to 70 °C and stirred for 2 h. After cooled to rt, the mixture was concentrated in vacuo to give a solid, purified by flash chromatography to afford tert-butyl ((6-chloro-lH-benzo[d]imidazol- 2-yl)methyl)c
Figure imgf000165_0001
To a solution of tert-butyl ((6-chloro-lH-benzo[d]imidazol-2-yl)methyl)carbamate (3.33 g, 11.8 mmol, 1.0 eq) in EA/MeOH (15 mL/60 mL) was added HC1/EA (20 mL). The mixture was stirred at rt overnight. The mixture was concentrated in vacuo to give a pink solid, purified by flash chromatography to afford (6-chloro- lH-benzo[d]imidazol-2-yl)methanamine as a pink oil (1.50 g, 69.8%).
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (96 mg, 0.30 mmol, 1.0 eq) in DCM (7.5 mL) were added HATU (171 mg, 0.45 mmol, 1.5 eq) and DIPEA (1.5 mL). The mixture was stirred at rt for 5 min. Then (6-chloro-lH-benzo[d]imidazol-2-yl)methanamine (66 mg, 0.36 mmol, 1.2 eq) was added, and the resulting mixture was stirred at rt overnight. The mixture was washed with ¾0 (5 mL x 2), sat.NaCl (5 mL), dried with Na2SC>4, concentrated to dryness to give a yellow solid. The residue was purified by flash chromatography to afford N-((6-chloro-lH-benzo[d]imidazol-2-yl)methyl)-5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (120 mg, 82.8%>). LRMS (M+H+) m/z calculated 483.1, found 483.1. ¾ NMR (DMSO-i/6, 400 MHz) δ 8.93 (d, 1H), 8.62 (d, 1H), 8.17 (s, 1H), 7.69 (dd, 1H), 7.53 (m, 3H), 7.27 (m, 5H), 6.57 (d, 1H), 6.39 (t, 1H), 5.18 (s, 2H), 4.82 (s, 2H), 4.10 (s, 2H).
Example 84: Preparation of N-((5-amino-3-methylpyrazin-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000166_0001
W"((5"amjno"3"methyl razjn"2~y|)methyl)~5"(4"^
Figure imgf000166_0002
To a solution of 5-bromo-6-methylpyrazin-2-amine (300 mg, 1.6 mmol, 1.0 eq) in NMP (12 mL) was added CuCN (287 mg, 3.2 mmol, 2.0 eq). The suspension was heated at 200 °C for 1 h in microwave. The reaction mixture was cooled to 25 °C, mixed with water (20 mL), NH3.H2O (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford 5-amino-3-methylpyrazine-2- carbonitrile as a brown solid (210 mg, 98%).
Figure imgf000166_0003
To a solution of 5-amino-3-methylpyrazine-2-carbonitrile (100 mg, 0.75 mmol, 1.0 eq) in Nl¾/MeOH (5 mL) was added Raney Ni (about 21 mg). The suspension was hydrogenated at rt overnight. The mixture was filtered. The filtered cake was washed with MeOH (10 mL). The filtrate was concentrated under reduced pressure to afford 5-(aminomethyl)-6-methylpyrazin-2-amine as a yellow solid (130 mg crude, quant).
Figure imgf000166_0004
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (100 mg, 0.31 mmol, 1.0 eq) in
DMF (2 mL) were added DIPEA (120 mg, 0.93 mmol, 3.0 eq) and HATU (141 mg, 0.37 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, 5-(aminomethyl)-6-methylpyrazin-2-amine (130 mg crude, 0.31 mmol, 1.0 eq) was then added and the mixture was stirred at rt for 2 h under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-amino-3-methylpyrazin-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a white solid (40 mg, 29%). LRMS (M+H+) m/z caculated 441.2, found 441.2. 'H NMR (DMSO-ifc, 400 MHz) δ 8.91 (t, 1 H), 8.84 (s, 1 H), 8.60 (s, 1 H), 8.02 (s, 1 H), 7.75 (dd, 1 H), 7.63 (s, 1 H), 7.50-7.30 (m, 1 H), 7.28-7.20 (m, 4 H), 6.39 (d, 1 H), 6.25-6.18 (m, 3 H), 5.04 (s, 2 H), 4.42 (d, 2 H), 3.98 (s, 2 H), 2.31 (s, 3 H).
Example 85: Preparation of N-((5-aminopyridin-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000167_0001
N ((5 amjnopyridin 2 yl)methy|) 5 (4 ((2 oxopyndin 1 (2H) y^methyl^enzyi^icotinamide
Figure imgf000167_0002
To the solution of 5-aminopicolinonitrile (500 mg, 4.2 mmol, 1.0 eq) in THF (10 mL) were added TEA (1.27 g, 12.6 mmol), DMAP (51.2 mg, 0.42 mmol), (Boc)20 (1.1 g, 5.04 mmol). The mixture was stirred at 60 °C for 2 h. After cooling to rt, 20 mL of H2O was added, the mixture was extracted with EtOAc (3x30 mL); the organics were washed with brine and dried over anhydrous sodium sulfate, filtered and concentrated, the residue was purified via silica column (PE/EtOAc = 3/1) to afford tert-butyl (6-cyanopyridin-3-yl)carbamate as a white solid (100 mg, 11%).
Figure imgf000167_0003
To a solution of tert-butyl (6-cyanopyridin-3-yl)carbamate (100 mg, 0.31 mmol, 1.0 eq) in MeOH.NF - (5 mL) was added Raney-nickel (50 mg). The mixture was stirred at rt under ¾ overnight. The mixture was filtered, the filtrate was concentrated to dryness to afford tert-butyl (6-(aminomethyl)pyridin-3-yl)carbamate as a yellow oil
Figure imgf000167_0004
A mixture of tert-butyl (6-(aminomethyl)pyridin-3-yl)carbamate (90 mg, 0.28 mmol, 1.0 eq), 5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (89 mg, 0.28 mmol, 1.0 eq), HATU (160 mg, 0.42 mmol, 1.5 eq) and TEA (85 mg, 0.84 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt overnight. The mixture was purified directly by flash chromatography to afford tert-butyl (6-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)pyridin-3-yl)carbamate as a colorless oil (60 mg, 41 >).
Figure imgf000168_0001
A mixture of tert-butyl (6-((5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamido)methyl)pyridin-3- yl)carbamate (60 mg, 0.11 mmol, 1.0 eq) in HCl/MeOH (25% w/w, 6mL) was stirred at rt overnight. The mixture was purified directly by flash chromatography to afford N-((5-aminopyridin-2-yl)methyl)-5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (25 mg, 52%). LRMS (M+H+) m/z calculated 426.19, found 426.2. ¾ NMR (CD3OD, 400 MHz) δ 9.12 (s, 1H), 8.86 (d, 2H), 7.98 (s, 1H), 7.82 (s, 1H), 7.60 (m, 2H), 7.27 (s, 4H), 6.79 (d, 2H), 6.72 (t, 1H), 5.25 (s, 2H), 4.64 (s, 2H), 4.22 (s, 2H).
Example 86: Preparation of N-(4-chloro-2-methylbenzyl)-5-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000168_0002
N (4 hioro 2 meth lbenzyi) 5 ^4 ^2 oxopyndin <| (2H) y|)methyl)benzy|)njcotinamjde
Figure imgf000168_0003
To a solution of 4-chloro-2-methylbenzaldehyde (2 g, 12.94 mmol, 1 eq) in MeOH were added NH2OH.HCI (1.35 g, 19.41 mmol, 1.5 eq) and TEA (2.61 g, 25.88 mmol, 2 eq). The mixture was stirred at 60 °C for 3 h, and then concentrated, purified by flash chromatography to afford 4-chloro-2-methylbenzaldehyde oxime as a yellow solid (1.1 g, 50 %).
Figure imgf000168_0004
To a solution of 4-chloro-2-methylbenzaldehyde oxime (400 mg, 2.36 mmol, 1 eq) in AcOH was added Zn (1.5 g, 23.6 mmol, 10 eq). The mixture was stirred at 60 °C for 2 h. The mixture was neutralized with
NH3.H2O, and extracted with EtOAc. The organics was concentrated to afford (4-chloro-2- methylphenyl)methanamine as a yellow oil (280 mg, 76 %>).
Figure imgf000169_0001
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (150 mg, 0.468 mmol, 1 eq) in DMF were added HATU (267 mg, 0.702 mmol, 1.5 eq), TEA (95 mg, 0.936 mmol, 2 eq), (4-chloro-2- methylphenyl)methanamine (150 mg, 0.936 mmol, 2 eq). The mixture was stirred at rt for 5 h, poured into water and extracted with EtOAc. The organics was concentrated and purified by prep-HPLC to afford N-(4- chloro-2-methylbenzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (80 mg, 37 %). LPvMS (M+H+) m/z calculated 458.2, found 458.2. ¾ NMR (d6-DMSO, 400 MHz) δ 9.14-9.11 (m, 1 H), 8.91 (s, 1 H), 8.67 (s, 1 H), 8.12 (s, 1 H), 7.77-7.75 (m, 1 H), 7.42-7.38 (m, 1 H), 7.26-7.20 (m, 7 H), 6.40- 6.38 (m, 1 H) , 6.23-6.20 (m, 1 H), 5.04 (s, 2 H), 4.43-4.42 (m, 2 H), 4.02 (s, 2 H), 2.08 (s, 3 H).
Example 87: Preparation of N-((6-amino-4-methylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000169_0002
To a solution of 6-amino-4-methylnicotinonitrile (200 mg, 1.5 mmol, 1.0 eq) in NHs/MeOH (5 mL) was added Raney Ni (about 20 mg). The suspension was hydrogenated at rt overnight. The mixture was filtered. The filtered cake was washed with MeOH (10 mL). The filtrate was concentrated under reduced pressure. The residue was purified via flash chromatography to afford 5-(aminomethyl)-4-methylpyridin-2-amine as a white solid (95 mg, 46%).
Figure imgf000169_0003
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (100 mg, 0.31 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (120 mg, 0.93 mmol, 3.0 eq) and HATU (141 mg, 0.37 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, 5-(aminomethyl)-4-methylpyridin-2-amine (47 mg, 0.34 mmol, 1.1 eq) was then added and the mixture was stirred at rt for 2 h under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((6-amino-4-methylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (70 mg, 51%). LRMS (M+H+) m/z caculated 440.2, found 440.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 8.90-8.75 (m, 2 H), 8.59 (d, 1 H), 8.01 (s, 1 H), 7.80-7.70 (m, 2 H), 7.45-7.35 (m, 1 H), 7.25-7.20 (m, 4 H), 6.39 (d, 1 H), 6.24 (s, 1 H), 6.21 (t, 1 H), 5.74 (s, 2 H), 5.04 (s, 2 H), 4.29 (d, 2 H), 3.98 (s, 2 H), 2.14 (s, 3 H).
Example 88: Preparation of N-((3-aminobenzo[d]isoxazol-5-yl)methyl)-5-(4-((2-oxopyridin-l
yl)methyl)benzyl)nicotinamide
N~((3~ami
Figure imgf000170_0001
Potassium tert-butylate (4.57 g, 40.8 mmol, 1.1 eq.) was suspended in THF (40 mL). Acetone oxime (2.97 g, 40.7 mmol, 1.1 eq.) was added and the mixture was stirred at rt for 20 min., followed by the addition of a solution of 2-fluoro-5-methylbenzonitrile (5.00 g, 37 mmol, 1.0 eq.) in THF (30 mL) dropwise. The mixture was stirred at rt for 3 h and then refluxed overnight. The dark brown solution was quenched with water (10 mL). The mixture was partitioned between saturated NaHC03 aqueous solution (50 mL) and EA (150 mL). The aqueous layer was extracted with EA (50 mL). The combined organic layers were dried over anhydrous Na2S04 and concentrated to afford brown oil. The crude oil was dissolved in EtOH (80 mL). H2O (53 mL) and cone. HC1 (27 mL) was added and the mixture was stirred at 90 °C for 2 h. Cooled to rt and the mixture was basified with NaOH aqueous solution to pH 10. The aqueous layer was extracted with EA (100 mL X3).The combined organic layers were dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography (PE/EA = 5/1, v/v) to afford 5-methylbenzo[d]isoxazol-3-amine as a white solid (2.5 g, 45.6%).
Figure imgf000170_0002
A mixture of 5-methylbenzo[d]isoxazol-3-amine (1.48 g, 10 mmol, 1.0 eq.), B0C2O (6.54 g, 30 mmol, 3.0 eq.), DMAP (122 mg, 1.0 mmol, 0.1 eq.), TEA (4.2 mL, 30 mmol, 3.0eq.) in DCM (30 mL) was refluxed for 18 h. The mixture was washed with water (30 mL X 2), dried over anhydrous Na2SC>4 and concentrated. The residue was purified via flash chromatography (PE/EA/DCM = 1/20/1-1/7/1, v/v/v) to afford tert-butyl [(tert- butoxy)-N-(5-methylbenz (3.2 g, 92%).
Figure imgf000171_0001
A mixture of tert-butyl [(tert-butoxy)-N-(5-methylbenzo[d]isoxazol-3-yl)carbonylamino]formate (1.04 g, 3 mmol, 1.0 eq.), NBS (536 mg, 3 mmol, 1.0 eq.), AIBN (53 mg, 0.32 mmol, 0.1 eq.) in CC14 (30 mL) was stirred at 85 °C for 5 h. Cooled to rt and the mixture was filtered. The filtrate was concentrated and the residue was purified via flash chromatography (PE/EA = 10/1, v/v) to afford tert-butyl {(fert-butoxy)-N-[5- (bromomethyl)benzo[d]isoxazol-3-yl]carbonylamino}formate as a white solid (970 mg, 75.8%).
Figure imgf000171_0002
A mixture of tert-butyl {(tert-butoxy)-N-[5-(bromomethyl)benzo[d]isoxazol-3-yl]carbonylamino}formate (602 mg, 1.4 mmol, 1.0 eq.), isoindoline-l,3-dione (310 mg, 2.1 mmol, 1.5 eq.), CS2CO3 (1.1 g, 3.4 mmol, 2.4 eq.) in DMF (10 mL) was stirred at 20 °C overnight. The mixture was partitioned between EA (20 mL) and water (20 mL). The aqueous layer was extracted with EA (20 mL X 2). The combined organic layers were dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography (PE/EA = 5/1, v/v) to afford tert-butyl ((tert-butoxy)-N-{5-[(l,3-dioxobenzo[c]azolidin-2-yl)methyl]benzo[d]isoxazol-3- yl}carbonylamino)formate as a white solid (616 mg, 88.8%).
Figure imgf000171_0003
To a solution of tert-butyl ((tert-butoxy)-N- {5-[(l,3-dioxobenzo[c]azolidin-2-yl)methyl]benzo[d]isoxazol-3- yl}carbonylamino)formate (320 mg, 0.65 mmol, 1.0 eq.) in n-BuOH (10 mL) was added NH2NH2.H2O (0.20 mL). The mixture was stirred at rt overnight. The white slurry was diluted with DCM (10 mL) and filtered. The filtrate was evaporated and to the residue was triturated with Et20 and dried to afford 3-(4-5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid as a white solid (120 mg, 70.6%>).
Figure imgf000172_0001
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (80 mg, 0.25 mmol, 1 eq), tert-butyl 5-(aminomethyl)benzo[d]isoxazol-3-ylcarbamate (66 mg, 0.25 mmol, 1.0 eq), HATU (114 mg, 0.30 mmol, 1.2 eq), DIPEA (0.5 mL) in DMF (3 mL) was stirred at rt overnight. The mixture was purified via prep- MPLC to afford tert-butyl 5-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)benzo[d]isoxazol-3-ylcarbamate as a white solid (96 mg, 67.8%).
Figure imgf000172_0002
To a solution of tert-butyl 5-((5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamido)methyl)benzo[d]isoxazol-3-ylcarbamate (96 mg, 0.17 mmol, 1 eq.) in DCM/MeOH (v/v=l/l, 6 mL) was added HCl/EA (5 N, 3 mL) and the mixture was stirred at rt overnight. The precipitate was collected by suction and washed with EA and DCM to afford N-((3-aminobenzo[d]isoxazol-5- yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide HC1 salt as a yellow solid (64 mg, 75.5%). LPvMS (M+H+) m/z calculated 466.2, found 466.2. ¾ NMR (CD3OD, 400 MHz) δ 9.18 (s, 1 H), 8.92 (s, 2 H), 7.91 (d, 1 H), 7.84 (s, 1 H), 7.72 (t, 1 H), 7.63 (d, 1 H), 7.41 (d, 1 H), 7.38-7.34 (m, 4 H), 6.74 (d, 1 H), 6.63 (t, 1 H), 5.28 (s, 2 H), 4.72 (s, 2 H), 4.31 (s, 2 H).
Example 89: Preparation of N-((6-methyl-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000172_0003
The mixture of 5-bromo-6-methyl-lH-pyrrolo[2,3-b]pyridine (2.11 g, 10.0 mmol, 1.0 eq) and CuCN (1.34 g, 15.0 mmol, 1.5 eq) in NMP (30 mL) was stirred at 170 °C for 6 h, then cooled, poured into 28% NH4OH (100 mL), and extracted with EA (50 mL X2). The extracts were washed with water, brine, dried over anhydrous sodium sulfate, filtered and concentrated to dryness to afford 6-methyl-lH-pyrrolo[2,3-b]pyridine-5- carbonitrile as a brown solid (0.9 g, 62%).
Figure imgf000173_0001
The mixture of 6-methyl-lH-pyrrolo[2,3-b]pyridine-5-carbonitrile (157 mg, 1.0 mmol, 1.0 eq) and Raney Ni in methanol (10 mL) and Ni¾/MeOH (5 N, 5 mL) was hydrogenated overnight. The mixture was filtered through celite, rinsed with DCM/MeOH (10/1), and the filtrate was concentrated to dryness to afford (6- methyl-lH-pyrrolo[2,3-b]pyridin-5-yl)methanamine as a yellow solid (136 mg, 84%).
Figure imgf000173_0002
5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (135 mg, 0.42 mmol, 1.0 eq) and HATU (239 mg, 0.63 mmol, 1.5 eq) were mixed in DCM (5 mL) and DIEA (0.23 mL), and the mixture was stirred for 30 min, then (6-methyl-lH-pyrrolo[2,3-b]pyridin-5-yl)methanamine (68 mg, 0.42 mmolo, 1.0 eq) was added. The reaction mixture was stirred for 1 h, quenched with water. The DCM layer was washed with water, brine and dried with anhydrous sodium sulfate, purified via flash chromatography to afford N-((6-methyl-lH- pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (70 mg, 35%). LRMS (M+H) + m/z calculated 464.2, found 464.2. 'HNMR (CD3OD, 400 MHz) δ 8.85 (s, 1 H), 8.57 (s, 1 H), 8.09 (s, 1 H), 7.93 (s, 1 H), 7.68 (d, 1 H), 7.52 (t, 1 H), 7.31-7.24 (m, 5 H), 6.57 (d, 1 H), 6.42 (d, 1 H), 6.38 (t, 1 H), 5.18 (s, 2 H), 4.69 (s, 2 H), 4.08 (s, 2 H), 2.64 (s, 3 H).
Example 90: Preparation of N-((3-chloro-6-methyl-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2- oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinamide
Figure imgf000173_0003
N ((3 chioro 6 methyl 1 H pyrrol°[2'3 b yHdin 5 yhmetnyh 5
(4"((2"oxopyndin"i ^H y|)methy|)benzy|)njcotinamjde
Figure imgf000174_0001
5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (135 mg, 0.42 mmol, 1.0 eq) and HATU (239 mg, 0.63 mmol, 1.5 eq) were mixed in DCM (5 mL) and DIEA (0.23 mL). The mixture was stirred for 30 min, then (6-methyl-lH-pyrrolo[2,3-b]pyridin-5-yl)methanamine (68 mg, 0.42 mmolo, 1.0 eq) was added, and the reaction was stirred for 1 h. Then it was quenched with water, the DCM layer was washed with water, brine and dried with anhydrous sodium sulfate, and purified via flash chromatography to afford N-((6-methyl-lH- pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (70 mg, 35%). LRMS (M+H) + m/z calculated 464.2, found 464.2. ¾ NMR (CD3OD, 400 MHz) δ 8.85 (s, 1 H), 8.57 (s, 1 H), 8.09 (s, 1 H), 7.93 (s, 1 H), 7.68 (d, 1 H), 7.52 (t, 1 H), 7.31-7.24 (m, 5 H), 6.57 (d, 1 H), 6.42 (d, 1 H), 6.38 (t, 1 H), 5.18 (s, 2 H), 4.69 (s, 2 H), 4.08 (s, 2 H), 2.64 (s, 3 H).
Example 91: Preparation of N-((6-methyl-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000174_0002
A mixture of 5-bromo-6-methyl-lH-indole (600 mg, 2.86 mmol, 1.0 eq) and CuCN (306 mg, 3.42 mmol, 1.2 eq) in NMP (10 mL) was stirred at 200 °C with microwave for 5 h. Then the mixture was poured into water (50 mL), extracted with EtOAc (50 mLx3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified by flash chromatography to afford 6-methyl-lH-indole-5-carbo
Figure imgf000174_0003
A mixture of 6-methyl-lH-indole-5-carbonitrile (120 mg, 0.77 mmol, 1.0 eq) and Raney Ni (50 mg) in methanol (15mL) was stirred at rt under ¾ atmosphere (1 atm) overnight. Then the mixture was filtered and concentrated to afford (6-methyl-lH-indol-5-yl)methanamine as a yellow solid (120 mg, 97%). The solid used for the next step without further purification.
Figure imgf000175_0001
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (240 mg, 0.75 mmol, 1.0 eq), (6- methyl-lH-indol-5-yl)methanamine (120 mg, 0.75 mmol, 1.0 eq), HATU (570 mg, 1.5 mmol, 2.0 eq) and DIEA (290 mg, 2.25 mmol, 3.0 eq) in DMF (10 mL) was stirred at rt for 2 h. The mixture was poured into water (50 mL), extracted with EtOAc (50 mLx3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2SC>4 and concentrated. The residue was purified by pre-HPLC to afford N-((6- methyl-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamideas a yellow solid (150 mg, 43%). LRMS (M+H+) m/z calculated 463.2, found 463.2. ¾ NMR (CD3OD, 400 MHz) δ 8.84 (s, 1 H), 8.56 (s, 1 H), 8.09 (s, 1 H), 7.68 (dd, 1 H), 7.53 (td, 1 H), 7.50 (s, 1 H), 7.29-7.23 (m, 5H), 7.16 (d, 1 H), 6.57 (d, 1 H), 6.40-6.37 (m, 2 H), 5.17 (s, 2 H), 4.66 (s, 2 H), 4.08 (s, 2 H), 2.44 (s, 3 H).
Example 92: Preparation of N-((3-chloro-6-methyl-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000175_0002
A mixture of N-((6-methyl-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (140 mg, 0.3 mmol, 1.0 eq) and NCS (40 mg, 0.3 mmol, 1.0 eq) in DCM (10 mL) was stirred at 25 °C overnight. Then the mixture was diluted with DCM (50 mL), washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified by flash chromatography to afford N-((3- chloro-6-methyl-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a yellow solid (15 mg, 10%). LRMS (M+H+) m/z calculated 497.2, found 497.1. ¾ NMR (CD3OD, 400 MHz) δ 8.84 (s, 1 H), 8.56 (s, 1 H), 8.09 (s, 1 H), 7.68 (dd, 1 H), 7.53 (td, 1 H), 7.47 (s, 1 H), 7.29-7.24 (m, 5H), 7.17 (d, 1 H), 6.57 (d, 1 H), 6.38 (td, 1 H), 5.18 (s, 2 H), 4.69 (s, 2 H), 4.08 (s, 2 H), 2.46 (s, 3 H).
Example 93: Preparation of N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000176_0001
■V pyrro|0[2'3 jjpyHdin 5 yl)methy|) 5 (4 ((2 oxop.
1 2H)"yl)methy|)benzy|)niCOtinalTiic|e
Figure imgf000176_0002
To the solution of lH-pyrrolo[2,3-b]pyridine-5-carbaldehyde (400 mg, 2.73 mmol, 1.0 eq) in MeOH (10 mL) was added sodium borohydride (208 mg, 5.46 mmol, 2.0 eq). The reaction was stirred for 1 h, and then purified via flash chromatography to afford (lH-pyrrolo[2,3-b]pyridin-5-yl)methanol as a white solid (310 mg, 76%).
Figure imgf000176_0003
To the suspension of (lH-pyrrolo[2,3-b]pyridin-5-yl)methanol (310 mg, 2.09 mmol, 1.0 eq) in dry DCM (10 mL) was added thionyl chloride (0.3 mL, 4.18 mmol, 2.0 eq) dropwise. The reaction was stirred for 3 h, and concentrated to dryness to afford 5-(chloromethyl)-lH-pyrrolo[2,3-b]pyridine as a white solid (420 mg, crude).
Figure imgf000176_0004
To the suspension of 5-(chloromethyl)-lH-pyrrolo[2,3-b]pyridine (420 mg, crude) in dry THF (15 mL) was added sodium hydride (130 mg, 60%>, 3.2 mmol) followed by di-tert-butyl iminodicarboxylate (600 mg, 2.7 mmol, 1.1 eq). The reaction was stirred for 16 h, quenched with saturated aqueous ammonium chloride, and purified via flash chromatography to afford (lH-pyrrolo[2,3-b]pyridin-5-yl)methanamine hydrochloeide as a white solid (190 mg, quant).
Figure imgf000177_0001
The mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (276 mg, 0.86 mmol, 1.0 eq) and HATU (495 mg, 1.30 mmol, 1.5 eq) in DMF (6 mL) and DIEA (1 mL) was stirred for 15 min. Then (1H- pyrrolo[2,3-b]pyridin-5-yl)methanamine hydrochloride (190 mg, 0.86 mmol, 1.0 eq) was added. The reaction was stirred for 1 h. The mixture was diluted with DCM (50 mL), washed with water, brine, and purified via flash chromatography to afford N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a white solid (290 mg, 75%). LRMS (M+H)+ m/z calculated 450.2, found 450.2. 'H NMR (DMSO-d6, 300 MHz) δ 11.59 (s, 1 H), 9.19 (t, 1 H), 8.87 (d, 1 H), 8.61 (d, 1 H), 8.20 (d, 1 H), 8.03 (d, 1 H), 7.88 (d, 1 H), 7.74 (dd, 1 H), 7.46-7.36 (m, 2 H), 7.25-7.19 (m, 4 H), 6.42-6.37 (m, 2 H), 6.20 (t, 1 H), 5.03 (s, 2 H), 4.55 (d, 2 H), 3.99 (s, 2 H).
Example 94: Preparation of N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000177_0002
The suspension of N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide (185 mg, 0.41 mmol, 1.0 eq) and NCS (55 mg, 0.41 mmol, 1.0 eq) in dry DCM (8 mL) was stirred at 25 °C for 8 h, and concentrated. Te residue was purified via flash chromatography to afford N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a white solid (100 mg, 50%). LRMS (M+H)+ m/z calculated 484.2, found 484.2. 'H NMR (DMSO-d6, 400 MHz) δ 11.96 (s, 1 H), 9.23 (t, 1 H), 8.87 (s, 1 H), 8.62 (s, 1 H), 8.30 (s, 1 H), 8.04 (s, 1 H), 7.87 (s, 1 H), 7.75 (d, 1 H), 7.68 (s, 1 H), 7.41 (t, 1 H), 7.24-7.20 (m, 4 H), 6.38 (d, 1 H), 6.20 (t, 1 H), 5.03 (s, 2 H), 5.00 (d, 2 H), 3.99 (s, 2 H).
Example 95: Preparation of N-((3-fluoro-4-methylpyridin-2-yl)methyl)-5-(4-((2-oxopyridin-l
yl)methyl)benzyl)nicotinamide
Figure imgf000178_0001
N ((3 fiuoro 4 methylpyndin 2 yl)methyl) 5 (4 ((2 oxopyndin 1 (2H) y^methyl^enzyi^icotinamide
Figure imgf000178_0002
To a mixture of 2-bromo-3-fluoro-4-methylpyridine (2.0 g, 10.5 mmol, 1.0 eq) and zinc cyanide (1.23 g, 10.5 mmol, 1.0 eq) in DMF (30 mL) was added palladium tetra(triphenylphosphine) (607 mg, 0.52 mmol, 0.05 eq). The mixture was degassed and then heated to 90 °C for 3 h. After this time, the mixture was diluted with water (200 mL) and EtOAc (200 mL), filtered and the resulting layers were separated. The aqueous layer was further extracted with EtOAc (2x100 mL). The combined extracts were washed with water (200 mL), dried over MgSO i, filtered and the solvent removed in vacuo. The resulting residue was purified by chromatograph on silica gel column (eluting with PE/EA = 20/1 to 6/1) to afford 3-fluoro-4-methylpicolinonitrile as a yellow oil (1.6 g, crude).
Figure imgf000178_0003
A stirred solution of 3-fluoro-4-methylpicolinonitrile (1.6 g, crude -30% purity, 1.0 eq) in 150 mL of ethanol and 6 mL (49 mmol) of cone HC1 was hydrogenated (50 psi) over 300 mg of 10% Pd/C for 20 h. The catalyst was removed by filtration and the solvents removed under reduced pressure. The resulting solid was purified by flash chromatography (MeCN/E O = 1/19 to 2/3) to afford (3-fluoro-4-methylpyridin-2-yl)methanamine HC1 salt as yellow solid (90 mg).
Figure imgf000178_0004
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (100 mg, 0.31 mmol, 1.0 eq), (3- fluoro-4-methylpyridin-2-yl)methanamine HCl salt (67 mg, 0.31 mmol, 1.0 eq), HATU (119 mg, 0.31 mmol, 1.0 eq) and DIEA (0.5 mL) in DCM (20 mL) was stirred at rt for 2 h. The mixture was evaporated under reduced pressure to give an oil, which was further purified by flash chromatography (MeCN/H20 = 1/9 to 1/1) to afford N-((3-fluoro-4-methylpyridin-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a white solid (51 mg, 36%). LRMS (M+H+) m/z calculated 443.1, found 443.1. 'H NMR (DMSO-d6, 400 MHz) δ 9.15 (t, 1 H), 8.86 (d, 1 H), 8.62 (d, 1 H), 8.19 (d, 1 H), 8.03 (d, 1 H), 7.75 (dd, 1 H), 7.42-7.37 (m, 1 H), 7.29-7.19 (m, 5 H), 6.39 (d, 1 H), 6.22-6.19 (m, 1 H), 5.04 (s, 2 H), 4.60 (d, 2 H), 3.99 (s, 2 H), 2.28 (d, 3 H).
Example 96: Preparation of N-((6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000179_0001
To a solution of 6-fluoro-l -(triisopropylsilyl)-lH-indole (2 g, 6.9 mmol, 1.0 eq) in THF (30 mL) was added s- BuLi (6.3 mL, 1.3 M, 1.2 eq) at -78 °C slowly. Then the mixture was stirred at this temperature for 1 h. DMF (1.5 g, 20.7 mmol, 3.0 eq) was added dropwise. The mixture was stirred at -78 °C for 1 h. Then the reaction was quenched by saturated aquoues NH4CI. The obtained mixture was extracted with EtOAc (50 mLx3). The organic layers were combined and washed with brine ,dried over Na2S04, filtered and concentrated. The residue was purified on silica gel column (PE/EtOAc = 100/1) to give 6-fluoro-l-(triisopropylsilyl)-lH- indole-5-carbaldehyde as a yellow oil (950 mg, 57%).
Figure imgf000179_0002
A mixture of 6-fluoro-l-(triisopropylsilyl)-lH-indole-5-carbaldehyde (780 mg, 2.45 mmol, 1.0 eq) and NH2OH.HCI (340 mg, 4.89 mmol, 2.0 eq) in NH3/MeOH (15% w/w, 10 mL) was stirred at rt overnight. The mixture was concentrated. The residue was purified directly on silica gel column (PE/EtOAc = 50/1) to afford 6-fluoro-lH-indole-5-carbaldehyde oxime as a yellow solid (460 mg, crude).
Figure imgf000180_0001
A mixture of 6-fluoro-lH-indole-5-carbaldehyde oxime (460 mg, 1.38 mmol, 1.0 eq) and Raney Ni (100 mg) in Nl¾/MeOH (15% w/w, 10 mL) was stirred at rt under ¾ atmosphere (1 atm) overnight. Then the mixture was filtered and concentrated to afford (6-fluoro-lH-indol-5-yl)methanamine as a gray solid (420 mg, 95%>). The solid
Figure imgf000180_0002
A mixture of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (312 mg, 0.98 mmol, 1.0 eq), (6- fluoro-lH-indol-5-yl)methanamine (160 mg, 0.98 mmol, 1.0 eq), HATU (559 mg, 1.47 mmol, 1.5 eq) and DIEA (379 mg, 2.94 mmol, 3.0 eq) in DMF (8 mL) was stirred at rt for 2 h. The mixture was purified directly by flash chromatography to afford N-((6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide as a yellow solid (100 mg, 22%>). LRMS (M+H+) m/z calculated 467.19, found 467.2. 'H NMR (CD3OD, 300 MHz) δ 8.84 (s, 1 H), 8.55 (s, 1 H), 8.09 (s, 1 H), 7.68 (dd, 1 H), 7.56-7.50 (m, 2 H), 7.29-7.22 (m, 5 H), 7.11 (d, 1 H), 6.57 (d, 1 H), 6.43 (d, 1 H), 6.38 (td, 1 H), 5.18 (s, 2 H), 4.69 (s, 2 H), 4.07 (s, 2 H).
Example 97: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000180_0003
A solution of N-((6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide (68 mg, 0.15 mmol, 1.0 eq) and NCS (39 mg, 0.29 mmol, 2.0 eq) in DCM (10 mL) was stirred at 20 °C overnight. Then the mixture was purified by flash chromatography to give N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a yellow oil (8.1 mg, 11%). LRMS (M+H+) m/z calculated 501.2, found 501.1. ¾ NMR (CD3OD, 400 MHz) δ 8.85 (s, 1 H), 8.57 (s, 1 H), 8.09 (s, 1 H), 7.67 (dd, 1 H), 7.55-7.51 (m, 2 H), 7.30-7.24 (m, 5 H), 7.14 (d, 1 H), 6.57 (d, 1 H), 6.39 (td, 1 H), 5.18 (s, 2 H), 4.71 (s, 2 H), 4.09 (s, 2 H).
Example 98: Preparation of N-(2-(guanidinooxy)ethyl)-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000181_0001
W"(2"(guanjdinooxy)ethyl)"5"(4"((2"oxopyrjdi
Figure imgf000181_0002
To a solution of 2-hydroxyisoindoline-l,3-dione (4.9 g, 30 mmol, 1.0 eq), benzyl (2-hydroxyethyl)carbamate (5.9 g, 30 mmol, 1.0 eq) and PPh3 (7.9 g, 30 mmol, 1.0 eq) in THF (100 mL) was added DIAD (6.1 g, 30 mmol, 1.0 eq). The mixture was stirred at rt overnight under N2. The reaction was mixed with saturated NaHC03 (100 mL) and extracted with EA (100 mL x 3). The combined organic layers were washed with brine (50 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified on silica gel column (DCM/MeOH = 400/1 to 100/1) to afford benzyl (2-((l,3-dioxoisoindolin-2-yl)oxy)ethyl)carbamate as a yellow solid (7.0 g, 69%).
Figure imgf000181_0003
To a solution of benzyl (2-((l,3-dioxoisoindolin-2-yl)oxy)ethyl)carbamate (6.0 g, 17.7 mmol, 1.0 eq) in EtOH (60 mL) and THF (60 mL) was added N2H4.H2O (8.8 g, 177 mmol, 10.0 eq). The mixture was stirred at rt for 2 h. The mixture was concentrated to dryness under reduced pressure. The residue was added DCM (100 mL) and stirred for 1 h. The suspension was filtered. The filtrate was concentrated to dryness under reduced pressure. The residue was purified via silica gel column (DCM/MeOH = 100/1 to 50/1) to afford benzyl (2- (aminooxy)ethyl)carbamate as a colorless oil (3.7 g, 85%>).
Figure imgf000181_0004
To a solution of benzyl (2-(aminooxy)ethyl)carbamate (3.7 g, 17.6 mmol, 1.0 eq) in DMF (50 mL) was added tert-butyl (((tert-butoxycarbonyl)amino)(lH-pyrazol-l-yl)methylene)carbamate (5.5 g, 17.6 mmol, 1.0 eq). The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was purified via silica gel column (PE/EA = 20/1 to DCM/MeOH = 300/1) to afford (N,N'-Di(tert- butoxycarbonyl))2-(benzyloxycarbonylamino)ethoxyguanidine as a colorless oil (1.2 g, 15%).
Boc Boc
N' H2' Pd/C N'
Cb-z O A Boc O i Boc
H2N- H H H H
To a solution of (N,N'-Di(tert-butoxycarbonyl))2-(benzyloxycarbonylamino)ethoxyguanidine (1.2 g, 2.66 mmol, 1.0 eq) in EtOH (20 mL) and THF (20 mL) was added Pd/C (120 mg, 10% Pd). The mixture was hydrogenated at rt for 1 h. The mixture was filtered. The filtrate was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford (N,N'-Di(tert-butoxycarbonyl))2- (amino)ethoxyguanidine as a white solid (320 mg, 38%>).
Figure imgf000182_0001
To a solution of 5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinic acid (60 mg, 0.19 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (73 mg, 0.57 mmol, 3.0 eq) and HATU (86 mg, 0.23 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (N,N'-di(tert-butoxycarbonyl))2- (amino)ethoxyguanidine (60 mg, 0.19 mmol, 1.0 eq) was then added and the mixture was stirred at rt overnight under N2. The reaction was purified via flash chromatography to afford the coupling product as a white solid (75 mg, 64%). To a solution of above product (75 mg, 0.12 mmol, 1.0 eq) in DCM (3 mL) was added TFA (1 mL). The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (5 mL), neutralized with saturated NaHCCb (5 mL) and concentrated. The residue was purified via Prep-HPLC to afford N-(2-(guanidinooxy)ethyl)-5-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (20 mg, 40%). LRMS (M+H+) m/z caculated 421.2, found 421.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 8.20 (d, 1 H), 8.64 (t, 1 H), 8.61 (d, 1 H), 7.99 (t, 1 H), 7.76 (dd, 1 H), 7.45-7.35 (m, 1 H), 7.30-7.20 (m, 4 H), 6.39 (d, 1 H), 6.21 (td, 1 H), 5.11 (brs, 2 H), 5.04 (s, 2 H), 4.38 (brs, 2 H), 3.99 (s, 2 H), 3.70 (t, 2 H), 3.50-3.40 (m, 2 H).
Example 99: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l
yl)methyl)pyridin-3-yl)methyl)nicotinamide
Figure imgf000183_0001
A mixture of methyl 6-methylnicotinate (2 g, 13.2 mmol, 1.0 eq), NBS (2.4 g, 13.2 mmol, 1.0 eq) and BPO (319 mg, 1.32 mmol, 0.1 eq) in CCI4 (40 mL) was stirred at 95 °C under N2 overnight. Then the reaction was quenched with water (10 mL), extracted with DCM (50 mLx3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified on silica gel column (PE/EtOAc = 20/1) to afford a yellow solid (780 mg, 26%).
Figure imgf000183_0002
A mixture of methyl 6-(bromomethyl)nicotinate (880 mg, 3.83 mmol, 1.0 eq), pyridin-2(lH)-one (545 mg, 5.74 mmol, 1.5 eq) and K2CO3 (1.06 g, 7.66 mmol, 2.0 eq) in CH3CN (30 mL) was stirred at 85 °C overnight. After cooled to rt, the mixture was filtered. The filtrate was concentrated. The residue was purified by flash chromatography to afford methyl 6-((2-oxopyridin-l(2H)-yl)methyl)nicotinate as a yellow solid (730 mg,
78%).
Figure imgf000183_0003
The solution of methyl 6-((2-oxopyridin-l(2H)-yl)methyl)nicotinate (1.15 g, 4.7 mmol, 1.0 eq) in THF (30 mL) was heated to reflux. Then MeOH was added slowly. The mixture was refluxed and stirred for 2 h. LCMS showed the reaction was completed. Then the mixture was cooled to rt and water (10 mL) was added. The obtained mixture was concentrated and the residue was diluted with MeOH (50 mL), filtered and the filtrate was concentrated to give l-((5-(hydroxymethyl)pyridin-2-yl)methyl)pyridin-2(lH)-one as a yellow solid (1.15 g, quant). The solid was used for the next step without further purification.
Figure imgf000184_0001
The mixture of l-((5-(hydroxymethyl)pyridin-2-yl)methyl)pyridin-2(lH)-one (1.15 g, 5.3 mmol, 1.0 eq) in DCM (20 mL) and SOCh. (10 mL) was stirred at rt overnight. Then the mixture was concentrated to give 1- ((5-(chloromethyl)pyridin-2- , 92%).
Figure imgf000184_0002
The mixture of ethyl 5-bromonicotinate (1 g, 4.3 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2- dioxaborolane) (1.1 g, 4.3 mmol, 1.0 eq), Pd(dppf)Cl2 (157 mg, 0.05 eq) and KOAc (1.26 g, 12.9 mmol, 3.0 eq) in dioxane (30 mL) was degassed with N2 and stirred at 85 °C overnight. Then the mixture was cooled, 1- ((5-(chloromethyl)pyridin-2-yl)methyl)pyridin-2(lH)-one (1.02 g, 4.3 mmol, 1.0 eq), Pd(dppf)Cl2 (157 mg, 0.05 eq) and an aqueous solution of Na2CC>3 (1.37 g, 12.9 mmol, 3.0 eq) in 10 mL of water were added. The mixture was degassed with N2 and stirred at 95 °C for 2 h. Then the mixture was cooled and concentrated, the residue was dissolved in EtOAc (150 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via silica gel column (PE/EtOAc = 1/1 -quant EtOAc) to afford methyl 5-((6-((2- oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinate as a brown solid (620 mg, 41%).
Figure imgf000184_0003
The solution of methyl 5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinate (620 mg, 1.78 mmol, 1.0 eq) in MeOH (5 mL) was treated with an aqueous solution of NaOH (213 mg, 5.33 mmol, 3.0 eq) in 10 mL of water. The mixture was stirred for 2 h at rt. Then the mixture was concentrated and the residue was acidified with 2 N HC1 till pH 3. The mixture was concentrated to give 5-((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)nicotinic acid as a gray solid (1.0 g, quant). The solid was used for the next step without further purification.
Figure imgf000185_0001
A mixture of (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (200 mg, 0.75 mmol, 1.0 eq), 5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinic acid (242 mg, 0.75 mmol, 1.0 eq), HATU (428 mg, 1.13 mmol, 1.5 eq) and DIEA (290 mg, 2.25 mmol, 3.0 eq) in DMF (10 mL) was stirred at rt for 2 h. The mixture was poured into water (50 mL). The appeared solid was filtered and dried to afford N- ((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)nicotinamide as a gray solid (200 mg, 47%). The solid was used for the next step without furth
Figure imgf000185_0002
The solutuion of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin- l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide (200 mg, 0.35 mmol, 1.0 eq) in 1 N HCl/EtOAc (8 mL) and MeOH (1 mL) was stirred at rt for 2 h. Then the mixture was concentrated and the residue was washed with MeOH to give N-((5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamide HC1 salt as a white solid (97.6 mg, 58%). LRMS (M+H+) m/z calculated 485.1, found 485.1. 'H NMR (DMSO-d6, 400 MHz) δ 13.21 (brs, 1 H), 9.62 (t, 1 H), 9.09 (s, 1 H), 8.91 (s, 1 H), 8.70 (s, 1 H), 8.58 (s, 1 H), 8.01 (d, 1 H), 7.94 (s, 1 H), 7.85 (d, 1 H), 7.54 (d, 1 H), 7.47 (t, 1 H), 7.35 (t, 2 H), 6.40 (d, 1 H), 6.29 (t, 1 H), 5.26 (s, 2 H), 4.80 (d, 2 H), 4.23 (s, 2 H).
Example 100: Preparation of 5-((2-(aminomethyl)pyridin-3-yl)methyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinami
Figure imgf000185_0003
A mixture of 3-methylpicolinonitrile (2 g, 16.9 mmol, 1.0 eq), NBS (3 g, 16.9 mmol, 1.0 eq) and BPO (41 1 mg, 1.7 mmol, 0.1 eq) in CCU (30 mL) was refluxed and stirred overnight. Then the mixture was cooled to rt and concentrated. The residue was diluted with water (50 mL), extracted with DCM (50 mLx3). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified on silica gel column (PE/EtOAc = 5/1) to afford 3-(bromomethyl)picolinonitrile as a brown solid (2.1 g, 63%).
Figure imgf000186_0001
The mixture of ethyl 5-bromonicotinate (1 g, 4.34 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l ,3,2- dioxaborolane) (l . lg, 4.34 mmol, 1.0 eq), Pd(dppf)Cl2 (157 mg, 5%) and KOAc (1.27 g, 13 mmol, 3.0 eq) in dioxane (36 mL) was degassed with N2 and stirred at 85 °C overnight. After cooling to rt, 3-
(bromomethyl)picolinonitrile (0.85 g, 4.34 mmol, 1.0 eq), Pd(dppf)Ci2 (157 mg, 5%) and an aqueous solution of Na2CC>3 (1.38 g, 13 mmol, 3.0 eq) in 10 mL of water were added. The mixture was degassed with N2 and stirred at 95 °C for 2 h. Then the mixture was concentrated. The residue was diluted with EtOAc (100 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via silica flash chromatography (PE/EtOAc = 1/1) to afford ethyl 5-((2-cyanopyridin-3-yl)methyl)nicotinate as a yellow solid (340 mg, 29%).
Figure imgf000186_0002
A mixture of ethyl 5-((2-cyanopyridin-3-yl)methyl)nicotinate (340 mg, 0.93 mmol, 1.0 eq), B0C2O (202 mg, 1.02 mmol, 1.1 eq) and Raney Ni (50 mg) in methanol (20 mL) was stirred at rt under ¾ atmosphere (1 atm) for 5 h. Then the mixture was filtered and concentrated. The residue was purified via silica gel column
(PE/EtOAc = 5/1) to afford ethyl 5-((2-(((tert-butoxycarbonyl)amino)methyl)pyridin-3-yl)methyl)nicotinate as a yellow oil (
Figure imgf000186_0003
To a solution of ethyl 5-((2-(((tert-butoxycarbonyl)amino)methyl)pyridin-3-yl)methyl)nicotinate (300 mg, 0.8 mmol, 1.0 eq) in MeOH (5 mL) and H2O (5 mL) was added NaOH (65 mg, 1.6 mmol, 2.0 eq). The mixture was stirred at rt for 2 h. Then the organic solvents were evaporated. To the residue was added cone. HC1 till pH 2. The obtained mixture was concentrated to afford 5-((2-(((tert-butoxycarbonyl)amino)methyl)pyridin-3- yl)methyl)nicotinic acid as a yellow solid (470 mg, crude, contained NaCl).
Figure imgf000187_0001
A mixture of 5-((2-(((tert-butoxycarbonyl)amino)methyl)pyridin-3-yl)methyl)nicotinic acid (235 mg, crude, 0.4 mmol, 1.0 eq), (3-chloro-lH-indol-5-yl)methanamine HCl salt (95 mg, 0.44 mmol, 1.1 eq), HATU (228 mg, 0.6 mmol,1.5 eq) and DIEA (258 mg, 2.0 mmol, 5.0 eq) in DMF (10 mL) was stirred at rt overnight. The mixture was purified directly by flash chromatography to afford fert-butyl ((3-((5-(((3-chloro-lH-indol-5- yl)methyl)carbamoyl)pyridin-3-yl)methyl)pyridin-2-yl)methyl)carbamate as a yellow solid (95 mg, 47%).
Figure imgf000187_0002
A mixture of tert-butyl ((3-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-yl)methyl)pyridin-2- yl)methyl)carbamate (95 mg, 0.19 mmol, 1.0 eq) in HCl/MeOH (20% w/w, 10 mL) was stirred at rt for 2 h. The mixture was purified directly by flash chromatography to afford 5-((2-(aminomethyl)pyridin-3- yl)methyl)-N-((3-chloro-lH-indol-5-yl)methyl)nicotinamide as a yellow solid (12 mg, 16%>). LRMS (M+H+) m/z calculated 406.14, found 406.1. ¾ NMR (CD3OD, 400 MHz) δ 8.89 (s, 1 H), 8.54 (s, 1 H), 8.46 (s, 1 H), 8.01 (s, 1 H), 7.57 (d, 1 H), 7.51 (s, 1 H), 7.35 (d, 1 H), 7.29-7.19 (m, 3 H), 4.66 (s, 2 H), 4.15 (s, 2 H), 3.89 (s, 2 H).
Example 101: Preparation of 5-((2-(aminomethyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide
Figure imgf000187_0003
A mixture of 5-((2-(((tert-butoxycarbonyl)amino)methyl)pyridin-3-yl)methyl)nicotinic acid (235 mg, crude, 0.4 mmol, 1.0 eq), (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (117 mg, 0.44 mmol, 1.1 eq), HATU (228 mg, 0.6 mmol,1.5 eq) and DIEA (155 mg, 1.2 mmol, 3.0 eq) in DMF (10 mL) was stirred at rt overnight. The mixture was purified directly by flash chromatography to afford tert-butyl ((3-((5- (((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)carbamoyl)pyridin-3-yl)methyl)pyridin-2- yl)
Figure imgf000188_0001
A mixture of tert-butyl ((3-((5-(((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)carbamoyl)pyridin-3-yl)methyl)pyridin-2-yl)methyl)carbamate (132 mg, 0.22 mmol, 1.0 eq) in HCl/MeOH (20% w/w, 10 mL) was stirred at rt for 2 h. The mixture was purified directly by flash chromatography to afford 5-((2-(aminomethyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide as a yellow solid (14 mg, 16%>). LRMS (M+H+) m/z calculated 407.14, found 407.1. ¾ NMR (CD3OD, 400 MHz) δ 8.87 (s, 1 H), 8.56 (s, 1 H), 8.46 (d, 1 H), 8.01 (s, 1 H), 7.85 (s, 1 H), 7.59 (d, 1 H), 7.48 (d, 1 H), 7.34 (dd, 1 H), 7.30-7.27 (m, 1 H), 4.18 (s, 2 H), 3.89 (s, 2 H), 3.35 (s, 2 H).
Example 102: Preparation of 5-(2-(aminomethyl)benzyl)-N-((3-chloro-lH-indol-5-yl)methyl)nicotinamide
Figure imgf000188_0002
The mixture of ethyl 5-bromonicotinate (1 g, 4.34 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2- dioxaborolane) (l . lg, 4.34 mmol, 1.0 eq), Pd(dppf)Cl2 (157 mg, 5%) and KOAc (1.27 g, 13 mmol, 3.0 eq) in dioxane (36 mL) was degassed with N2 and stirred at 85 °C overnight. After cooling to rt, 2- (bromomethyl)benzonitrile (0.85 g, 4.34 mmol, 1.0 eq), Pd(dppf)Ci2 (157 mg, 5%>) and an aqueous solution of Na2CC>3 ( 1.38 g, 13 mmol, 3.0 eq) in 10 mL of water were added. The mixture was degassed with N2 and stirred at 95 °C for 2 h. Then the mixture was concentrated. The residue was diluted with EtOAc (100 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via silica flash chromatography (PE/EtOAc = 5/1) to afford ethyl 5-(2-cyanobenzyl)nicotinate as a yellow oil (750 mg, 65%).
Figure imgf000189_0001
A mixture of ethyl 5-(2-cyanobenzyl)nicotinate (750 mg, 2.8 mmol, 1.0 eq), B0C2O (676 mg, 3.1 mmol, 1.1 eq) and Raney Ni (100 mg) in methanol (20 mL) was stirred at rt under ¾ atmosphere (1 atm) overnight. Then the mixture was filtered and concentrated. The residue was purified via silica gel column (PE/EtOAc = 5/1) to afford ethyl 5-(2-(((tert-butoxycarbonyl)amino)methyl)benzyl)nicotinate as a yellow oil (520 mg, 50%).
Figure imgf000189_0002
To a solution of ethyl 5-(2-(((tert-butoxycarbonyl)amino)methyl)benzyl)nicotinate (350 mg, 0.95 mmol, 1.0 eq) in MeOH (5 mL) and H2O (5 mL) was added NaOH (76 mg, 1.89 mmol, 2.0 eq). The mixture was stirred at rt for 1 h. Then the organic solvents were evaporated. To the residue was added cone. HC1 till pH 2. The obtained mixture was concentrated to afford 5-(2-(((tert-butoxycarbonyl)amino)methyl)benzyl)nicotinic acid as a yellow solid (500 mg, crude, contained NaCl).
Figure imgf000189_0003
A mixture of 5-(2-(((tert-butoxycarbonyl)amino)methyl)benzyl)nicotinic acid (250 mg, crude, 0.42 mmol, 1.0 eq), (3-chloro-lH-indol-5-yl)methanamine HC1 salt (100 mg, 0.46 mmol, 1.1 eq), HATU (239 mg, 0.63 mmol,1.5 eq) and DIEA (162 mg, 1.26 mmol, 3.0 eq) in DMF (10 mL) was stirred at rt overnight. The mixture was purified directly by flash chromatography to afford tert-butyl 2-((5-(((3-chloro-lH-indol-5- yl)methyl)carb
Figure imgf000189_0004
A mixture of tert-butyl 2-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3- yl)methyl)benzylcarbamate (132 mg, 0.26 mmol, 1.0 eq) in HCl/MeOH (25% w/w, 10 mL) was stirred at rt for 2 h. The mixture was purified directly by flash chromatography to afford 5-(2-(aminomethyl)benzyl)-N- ((3-chloro-lH-indol-5-yl)methyl)nicotinamide as a yellow solid (27 mg, 26%). LRMS (M+H+) m/z calculated 405.15, found 405.1. ¾ NMR (DMSO-d6, 300 MHz) δ 11.37 (s, 1 H), 9.29 (t, 1 H), 8.94 (s, 1 H), 8.58 (s, 1 H), 8.34 (brs, 3 H), 8.00 (s, 1 H), 7.51-7.33 (m, 5 H), 7.21-7.16 (m, 2 H), 4.56 (d, 2 H), 4.19 (s, 2 H), 4.05- 4.00 (m, 2 H).
Example 103: Preparation of N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyrazin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000190_0001
A mixture of 5-(4-(hydroxymethyl)benzyl)nicotinic acid (500 mg, 2.0 mmol, 1.0 eq), (3-chloro-lH-indol-5- yl)methanamine HCl salt (446 mg, 2.0 mmol, 1.0 eq), HATU (782 mg, 2.0 mmol, 1.0 eq) and DIEA (2.0 mL) in DMF (6 mL) was stirred at rt for 2 h and then purified by flash chromatography (MeCN/H20 = 1/4 to 4/1) to afford N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-(hydroxymethyl)benzyl)nicotinamide as white solid (389 mg, 46%).
Figure imgf000190_0002
To a solution of N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-(hydroxymethyl)benzyl)nicotinamide (380 mg, 0.93 mmol, 1.0 eq) in 15 ml of DCM was added dropwise SOCb (1 mL) in ice-water bath. The mixture was then stirred at rt for 1 h and evaporated under reduced pressure to give an oil, which was purified by flash chromatography (MeCN/H20 = 1/2 to 19/1) to afford N-((3-chloro-lH-indol-5-yl)methyl)-5-(4- (chloromethyl)be
Figure imgf000191_0001
A solution of N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-(chloromethyl)benzyl)nicotinamide (90 mg, 0.21 mmol, 1.0 eq), pyrazin-2(lH)-one (41 mg, 0.42 mmol, 2.0 eq) and K2C03 (58 mg, 0.42 mmol, 2.0 eq) in MeCN (10 mL) was stirred at 80 °C for 2 h and allowed to cool. The mixture was concentrated. The residue was purified by flash chromatography (MeCN/l hO = 1/2 to 19/1) to afford N-((3-chloro-lH-indol-5- yl)methyl)-5-(4-((2-oxopyrazin-l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (6.3 mg, 6%). LRMS (M+H+) m/z calculated 484.1, found 484.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 8.72 (s, 1 H), 8.44 (s, 1 H), 7.97 (s, 1 H), 7.95 (d, 1 H), 7.47 (d, 1 H), 7.40 (s, 1 H), 7.26-7.20 (m, 4 H), 7.16-7.10 (m, 3 H), 7.10-7.08 (m, 1 H), 5.00 (s, 2 H), 4.55 (s, 2 H), 3.96 (s, 2 H).
Example 104: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyrazin-l(2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000191_0002
A mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4- (chloromethyl)benzyl)nicotinamide (50 mg, 0.098 mmol, 1.0 eq), pyrazin-2(lH)-one (11 mg, 0.11 mmol, 1.2 eq), and K2CO3 (27 mg, 0.19 mmol, 2.0 eq) in MeCN (2 mL) was heated at reflux for 18 h. The resulting suspension was concentrated in vacuo. The residue was purified by flash chromatography (MeCN/l hO = 1/2 to 19/1) to give N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyrazin- l(2H)-yl)methyl)benzyl)nicotinamide as a white solid (37 mg, 66%).
Figure imgf000192_0001
A solution of N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-5-(4-((2-oxopyrazin- 1 (2H)- yl)methyl)benzyl)nicotinamide (37 mg, 0.065 mmol, 1.0 eq) in MeOH (2 mL) and HC1/EA (3 N, 3 mL) was stirred at 40 °C for 3 h. Then the mixture was concentrated. The residue was purified by flash chromatography (MeCN/H20 = 1/9 to 2/1) to afford N-((5-chloro-l H-indazol-3 -yl)methyl)-5-(4-((2-oxopyrazin-l(2H)- yl)methyl)benzyl)nicotinamide as a white solid (19 mg, 60%). LRMS (M+H+) m/z calculated 485.1, found 485.1. 'H NMR (DMSO-ifc, 400 MHz) δ 13.07 (s, 1 H), 9.28 (t, 1 H), 8.86 (d, 1 H), 8.62 (d, 1 H), 8.04 (s, 1 H), 8.01 (s, 1 H), 7.89 (d, 1 H), 7.73 (d, 1 H) , 7.52 (d, 1 H), 7.34-7.27 (m, 2 H), 7.25 (m, 4 H), 5.02 (s, 2 H), 4.77 (d, 2 H), 3.99 (s, 2 H).
Example 105: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxopyrrolidin-l - yl)benzyl)nicotinamide
Figure imgf000192_0002
The mixture of pyrrolidin-2-one (3.0 g, 35.3 mmol, 1.0 eq), methyl 4-bromobenzoate (8.8 g, 40.9 mmol, 1.16 eq), tris(dibenzylideneacetone)dipalladium(0) (807 mg, 0.88 mmol, 2.5%), Xantphos (1.53 g, 2.65 mmol, 7.5%)) and cesium carbonate (1.60 g, 49.4 mmol, 1.4 eq) in 1,4-dioxane (120 mL) was degassed and refluxed under nitrogen overnight. Then it was concentrated, and the residue was portioned between water and DCM. The DCM layer was separated and washed with water, brine, dried over sodium sulfate, and purified silica gel column (PE/EA = 1/1) to afford methyl 4-(2-oxopyrrolidin-l-yl)benzoate as a white solid (7.0 g, 90%>).
Figure imgf000193_0001
The mixture of methyl 4-(2-oxopyrrolidin-l -yl)benzoate (7.5 g, 34.2 mmol, 1.0 eq), sodium borohydride (7.8 g, 205.6 mmol, 6.0 eq) in THF (200 mL) was heated to reflux, then MeOH was added drop wise carefully until the reaction was complete. After cooling, the mixture was filtered through celite, concentrated, and the residue was dissolved in DCM (100 mL), washed with brine, dried over MgSO i, abd purified silica gel column (PE/EA = 1/2) to afford l-(4-(hydroxymethyl)phenyl)pyrrolidin-2-one as a white solid (1.68 g, 25%). LRMS (M+H+) m/z calculated 444.2, found 444.2.
Figure imgf000193_0002
To a solution of 1 -(4-(hydroxymethyl)phenyl)pyrrolidin-2-one (208 mg, 1.1 mmol, 1.0 eq) in dry DCM (10 mL) was added thionyl chloride (0.16 mL, 2.2 mmol, 2.0 eq). The reaction mixture was stirred for 30 min, concentrated, and the residue was purified via flash chromatography (PE/DCM/EA = 1/1/1) to afford l-(4- (chloromethyl)phenyl)pyrrolidin-2-one as a white solid (210 mg, 92%). LRMS (M+H+) m/z calculated 444.2, found 444.2.
Figure imgf000193_0003
The mixture of ethyl 5-bromonicotinate (345 mg, 1.5 mmol, 1.5 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'- bi(l,3,2-dioxaborolane) (381 mg, 1.5 mmol, 1.5 eq), [1,1 '- bis(diphenylphosphino)ferrocene]dichloropalladium(II) (55 mg, 5%>) and potassium acetate (441 mg, 4.5 mmol, 4.5 eq) in dioxane (10 mL) was degassed with N2 and stirred at 80 °C for 3.5 h, then cooled, l-(4- (chloromethyl)phenyl)pyrrolidin-2-one (210 mg, 1.0 mmol, 1.0 eq), [1,1 '- Bis(diphenylphosphino)ferrocene]dichloropalladium(II) (55 mg, 5%>) and an aqueous solution of sodium carbonate (447 mg, 4.5 mmol, 4.5 eq) in 5 mL of water were added. The mixture was degassed and stirred at 95 °C under N2 overnight. The reaction mixture was cooled and filtered through celite, concentrated, and the residue was dissolved in DCM (150 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via flash chromatography (5%> MeOH in DCM) to afford ethyl 5-(4-(2-oxopyrrolidin-l - yl)benzyl)nicotinate as a yellow solid (170 mg, 52%>).
Figure imgf000194_0001
The mixture of ethyl 5-(4-(2-oxopyrrolidin-l-yl)benzyl)nicotinate (170 mg, 0.52 mmol, 1.0 eq) and sodium hydroxide (42 mg, 1.04 mmol, 2.0 eq) in MeOH (3 mL) and water (3 mL) was stirred at rt for 1.5 h, then concentrated. The residue was diluted with water (2 mL) and washed with EA once, then acidified with concentrated HC1 till pH 2. The organic phase was concentrated in vacuo to afford 5-(4-(2-oxopyrrolidin-l - yl)b
Figure imgf000194_0002
The mixture of 5-(4-(2-oxopyrrolidin-l -yl)benzyl)nicotinic acid (abtanied above), HATU (300 mg, 0.78 mmol, 1.5 eq) and Et3N (0.3 mL) in DCM (5 mL) was stirred for 15 min, (5-chloro-l-(tetrahydro-2H-pyran-2- yl)-lH-indazol-3-yl)methanamine (138 mg, 0.52 mmol, 1.0 eq) was added. The reaction mixture was stirred for 1 h, quenched with water, and the DCM layer was washed with brine, dried over MgSO i, purified via silica flash chromatography (5% MeOH in DCM) to afford 5-(4-(2-oxopyrrolidin-l-yl)benzyl)-N-((l - (tetrahydro-2H-p 70%).
Figure imgf000194_0003
The mixture of 5-(4-(2-oxopyrrolidin-l -yl)benzyl)-N-((l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)nicotinamide (200 mg, 0.36 mmol, 1.0 eq) in MeOH (3 mL) was treated with 5 NHCl/dioxane and stirred for 1 h. then it was concentrated and purified via reverse flash chromatography to afford N-((1H- indazol-3-yl)methyl)-5-(4-(2-oxopyrrolidin-l-yl)benzyl)nicotinamide as a white solid (140 mg, 82%). LRMS (M+H+) m/z calculated 460.1, found 460.1. ¾ NMR (CDC13 + CD3OD, 400 MHz) δ 8.83 (s, 1 H), 8.52 (s, 1 H), 8.02 (s, 1 H), 7.84 (s, 1 H), 7.50 (d, 2 H), 7.40 (d, 1 H) , 7.33 (d, 1 H) , 7.19 (d, 2 H) , 4.91 (d, 2 H), 4.02 (s, 2 H), 3.86 (t, 2 H), 2.61 (t, 2 H), 2.19-2.15 (m, 3 H).
Example 106: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxopyridin-l(2H)- yl)benzyl)nicotinamide
Figure imgf000195_0001
chioro 1 H indazoi 3 yi^methyl) 5 (4 (2 oxopyridin <| (2H) y|)benzy|^nicotinamide
Figure imgf000195_0002
The mixture of pyridin-2(lH)-one (0.95 g, 10.0 mmol, 1.0 eq), 4-fluorobenzaldehyde (1.24 g, 10.0 mmol, 1.0 eq) and Cs2C03 (4.2 g, 13.0 mmol, 1.3 eq) in DMF (50 mL) was stirred at 120 °C overnight. Then it was cooled, poured into water, extracted with EA (50 mLX2), washed with water, brine and purified by reverse flash chromatography to afford 4-(2-oxopyridin-l(2H)-yl)benzaldehyde as a white solid (600 mg).
Figure imgf000195_0003
The mixture of 4-(2-oxopyridin-l(2H)-yl)benzaldehyde (300 mg, 1.5 mmol, 1.0 eq), NaBH4 (114 mg, 3.0 mmol, 2.0 eq) in MeOH (7 mL) was stirred at rt overnight. Then it was concentrated, and the residue was purified via silica flash chromatography to afford l -(4-(hydroxymethyl)phenyl)pyridin-2(lH)-one as a white solid (270 mg, 89%).
Figure imgf000195_0004
The solution of l-(4-(hydroxymethyl)phenyl)pyridin-2(lH)-one (270 mg, 1.34 mmol, 1.0 eq) in dry DCM (10 mL) was added SOCb (0.19 mL, 2.68 mmol, 2.0 eq). The reaction mixture was stirred for 60 min, and concentrated. The residue was purified silica flash chromatography (EA) to afford l-(4- (chloromethyl)phenyl)pyridin-2(lH)-one as a white solid (290 mg, quantitative).
Figure imgf000196_0001
The mixture of ethyl 5-bromonicotinate (345 mg, 1.5 mmol, 1.5 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'- bi(l,3,2-dioxaborolane) (381 mg, 1.5 mmol, 1.5 eq), Pd(dppf)Cl2 (55 mg, 5%) and KOAc (441 mg, 4.5 mmol, 4.5 eq) in dioxane (10 mL) was degassed with N2 and stirred at 80 °C for 3.5 h. after cooling, l-(4- (chloromethyl)phenyl)pyridin-2(lH)-one (210 mg, 1.0 mmol, 1.0 eq), Pd(dppf)Cl2 (55 mg, 5%) and an aqueous solution of Na2C03 (447 mg, 4.5 mmol, 4.5 eq) in 5 mL of water were added to this mixture. The mixture was degassed and stirred at 95 °C under N2 overnight. It was cooled and filtered through celite, concentrated. And the residue was dissolved in DCM (150 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via silica flash chromatography (DCM/MeOH = 20/1) to afford ethyl 5-(4-(2-oxopyridin-l(2H)-yl)benzyl)nicotinate as a yellow solid (170 mg, 52%).
Figure imgf000196_0002
The solution of ethyl 5-(4-(2-oxopyridin-l(2H)-yl)benzyl)nicotinate (300 mg, 0.898 mmol, 1.0 eq) in MeOH (4 mL) was treated with an aqueous solution of NaOH (72 mg, 1.796 mmol, 2.0 eq) in 3 mL of water. The mixture was stirred for 1 h and concentrated. The residue was acidified with 2 N HCl till pH 1 to afford 5-(4- (2-ox
Figure imgf000196_0003
The mixture of 5-(4-(2-oxopyridin-l(2H)-yl)benzyl)nicotinic acid (80 mg, 0.26 mmol, 1.0 eq) and HATU (148 mg, 0.39 mmol, 1.5 eq) in DMF (3 mL) and DIPEA (0.3 ml) was stirred for 30 min, and then (5-chloro- l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (69 mg, 0.26 mmol, 1.0 eq) was added. The reaction was stirred at rt for 1 h, quenched with water, and extracted with DCM. The DCM layer was washed with brine, and purified via flash chromatography to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)-5-(4-(2-oxopyridin-l(2H)-yl)benzyl)nicotinamide as a white solid (100 mg, 69%>).
Figure imgf000197_0001
The solutuion of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-(2-oxopyridin- l(2H)-yl)benzyl)nicotinamide (100 mg, 0.18 mmol, 1.0 eq) in MeOH (3 mL) was treated with 2 N HC1/EA and stirred for 30 min. After concentration, the residue was purified by flash chromatography to afford N-((5- chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxopyridin-l(2H)-yl)benzyl)nicotinamide as a white solid (40 mg, 47%). LRMS (M+H+) m/z calculated 470.1, found 470.1. ¾ NMR (DMSO-d6, 400 MHz) δ 13.10 (s, 1 H), 9.34 (s, 1 H), 8.89 (s, 1 H), 8.70 (s, 1 H), 8.15 (s, 1 H), 7.92 (s, 1 H), 7.74-7.15 (m, 8 H), 6.56- 6.38 (m, 1 H), 6.28 (s, 1 H), 4.80 (s, 2 H), 4.10 (s, 3 H).
Example 107: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((3- oxomorpholino)methyl)benzyl)nicotinamide
Figure imgf000197_0002
To a solution of methyl 4-(bromomethyl)benzoate (2.3 g, 0.01 mol) in dry DCM (80 mL) was added DIBAL- H (22 mL, 0.03 mol) at -78 °C under N2. The mixture was stirred at -78 °C for 1.5 h. The reaction mixture was quenched by careful addition of H2O (50 mL), and then extracted with DCM (100 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated to afford (4- (bromomethyl)phe
Figure imgf000197_0003
A mixture of (4-(bromomethyl)phenyl)methanol (200 mg, 1.0 mmol), (5-(ethoxycarbonyl)pyridin-3- yl)boronic acid (195 mg, 1.0 mmol), Pd(PPh4)3 (116 mg, 0.1 mmol), and Na2C03 (318 mg, 3.0 mmol) in 1,4- dioxane (10 mL) and H2O (2 mL) was heated at 85 °C overnight under N2. The mixture was diluted with water (100 mL) and extracted with EA (50 mL X 3). The combined organic layers were dried over anhydrous sodium sulfate and concentrated. The residue was purified via prep-HPLC to affored ethyl 5-(4- (hydroxymethyl)benzyl)
Figure imgf000198_0001
A mixture of ethyl 5-(4-(hydroxymethyl)benzyl)nicotinate (100 mg, 0.36 mmol), and SOCb (0.04 mL, 0.36 mmol) in DCM (5 mL) was stirred at rt for 30 min. The mixture was concentrated to afford ethyl 5-(4- (chloromethyl)benzyl)nic
Figure imgf000198_0002
To a suspension of NaH (20.8 mg, 0.52 mmol) in dry DMF (2 mL) was added morpholin-3-one (52 mg, 0.52 mmol) at rt. The mixture was stirred at rt for 30 min, then ethyl 5-(4-(chloromethyl)benzyl)nicotinate (100 mg, 0.34 mmol) in 2 mL of DMF was added. The mixture was stirred at rt for 2 h, and then concentrated to afford 5-(4-((3-ox ).
Figure imgf000198_0003
A mixture of 5-(4-((3-oxomorpholino)methyl)benzyl)nicotinic acid (56 mg, 0.17 mmol), (5-chloro-l - (tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (53 mg, 0.18 mmol), HATU (98 mg, 0.28 mmol) and TEA (0.07 mL, 0.51 mmol) in DMF (5 mL) was stirred at rt for 2 h. The mixture was concentrated and purified via prep-HPLC to affored N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4- ((3-oxomorpholino)methyl)benzyl)nicotinamide as a white solid (30 mg, 30%).
Figure imgf000199_0001
A mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((3- oxomorpholino)methyl)benzyl)nicotinamide (20 mg, 0.03 mmol) in HCl/MeOH (5 mL) was stirred at rt for overnight. The mixture was concentrated and purified by prep-HPLC to afford N-((5-chloro-lH-indazol-3- yl)methyl)-5-(4-((3-oxomorpholino)methyl)benzyl)nicotinamide as a white solid (6 mg, 30%). LRMS (M+H+) m/z calculated 490, found 490.1. ¾ NMR (CD3OD, 400 MHz) δ 9.18 (s, 1 H), 8.92 (d, 2 H), 7.92 (s, 1 H), 7.52-7.50 (d, 1 H), 7.44-7.42 (d, 1 H), 7.33-7.32 (m, 4 H), 4.97 (s, 2 H), 4.62 (s, 2 H), 4.31 (s, 2 H), 4.20 (s, 2 H), 3.87-3.85 (m, 2 H). Example 108: Preparation of 5-(4-((lH-pyrazol-l-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide
Figure imgf000199_0002
5 (4 ((1 H pyrazoi 1 yljmethyljbenzyij N ((5 chioro w indazoi 3 yijmethyljnicotinamjde
Figure imgf000199_0003
To a solution of methyl 4-(bromomethyl)benzoate (5.0 g, 21.8 mmol, 1.0 eq) in MeCN (5 mL), lH-pyrazole (2.2 g, 32.7 mmol, 1.5 eq) and K2CO3 (6.0 g, 43.7 mmol, 2.0 eq) were added and the mixture was stirred at 70 °C for 5 h. The mixture was concentrated, poured into water, extracted with EA (3x100 mL). The combined organic layers were washed with brine, concentrated, purified by column chromatography (PE/EA = 5: 1) to afford methyl 4-((lH-pyrazol-l-yl 3.8%).
Figure imgf000199_0004
To a solution of methyl 4-((lH-pyrazol-l -yl)methyl)benzoate (800 mg, 3.70 mmol, 1.0 eq) in THF (10 mL) was added L1AIH4 (281 mg, 7.4 mmol) at 0 °C. The mixture was stirred at 0 °C for 2 h. The reaction was quenched with Na2SO4-10H2O, poured into water, extracted with EA (3 x50 mL). The combined organic layers were washed with brine, and concentrated to afford (4-((lH-pyrazol-l-yl)methyl)phenyl)methanol as a yellow oil (600 mg, 86%).
Figure imgf000200_0001
To a solution of (4-((lH-pyrazol-l -yl)methyl)phenyl)methanol (600 mg, 3.19 mmol, 1.0 eq) in THF (10 mL) was added PBr3 (2.6 g, 9.56 mmol) at 0 °C and the mixture was stirred at that temperature for 2 h. The mixture was poured into ice-water, extracted with DCM (3x50 mL). The combined organic layers were washed with saturated aqueous NaHC03 and brine, dried, and concentrated to afford l-(4-(bromomethyl)benzyl)-lH- pyrazole as a white solid (600 mg, 75%).
Figure imgf000200_0002
A mixture of (5-(ethoxycarbonyl)pyridin-3-yl)boronic acid (233 mg, 1.19 mmol, 1.0 eq) and l-(4- (bromomethyl)benzyl)-lH-pyrazole (300 mg, 1.19 mmol, 1.0 eq), Pd(PPli3)4 (140 mg, 0.12 mmol, 0.1 eq) and Na2C03 (140 mg, 3.57 mmol, 3.0 eq) in dioxane/H20 (7 mL / 1 mL) was stirred at 100 °C under N2 atmosphere overnight. Then the mixture was filtered and the filtrate concentrated. The residue was poured into water, extracted with EA (3x20 mL). The combined organic layers were washed with brine, and concentrated. The residue was purified by prep-TLC to afford ethyl 5-(4-((lH-pyrazol-l-yl)methyl)benzyl)nicotinate as a brown oil (100 m
Figure imgf000200_0003
5-(4-((lH-Pyrazol-l -yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)nicotinamide (8 mg, HCI salt) was prepared from 5-(4-((lH-pyrazol-l -yl)methyl)benzyl)nicotinate as described for N-((5-chloro-lH- indazol-3-yl)methyl)-5-(2-chlorobenzyl)nicotinamide. LRMS (M+H+) m/z caculated 456.15, found 456.2. ¾
NMR (CD3OD, 400 MHz) δ 9.06 (s, 1 H), 8.79 (d, 2 H), 7.99 (s, 1 H), 7.83(d, 2 H), 7.41 (d, 1 H), 7.28-7.18
(m, 5 H), 6.50 (s, 1 H), 5.41 (s, 2 H), 4.84 (s, 2 H), 4.20 (s, 2 H). Example 109: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopiperazin-l - yl)methyl)benzyl)nicotinamide
Figure imgf000201_0001
To a solution of NaH (40 mg, 0.99 mmol) in DMF (3 mL) was added fert-butyl 3-oxopiperazine-l - carboxylate (330 mg, 1.65 mmol). The mixture was stirred at rt for 0.5 h, N-((5-chloro-lH-indazol-3- yl)methyl)-5-(4-(chloromethyl)benzyl)nicotinamide (70 mg, 0.16 mmol, 1.0 eq) was then added. The mixture was stirred at rt for 1 h. Water (0.5 mL) was added into the reaction mixture to quench the reaction. The mixture was evaporated to dryness, and the residue was purified by prep-HPLC to afford tert-butyl 4-(4-((5- (((5-chloro-lH-indazol-3-yl)methyl)carbamoyl)pyridin-3-yl)methyl)benzyl)-3-oxopiperazine-l -carboxylate as a yellow soli
Figure imgf000201_0002
A mixture of tert-butyl 4-(4-((5-(((5-chloro-l H-indazol-3-yl)methyl)carbamoyl)pyridin-3-yl)methyl)benzyl)- 3-oxopiperazine-l -carboxylate (18 mg, 0.036 mmol, 1.0 eq) in HCl/MeOH (25% w/w, 4 mL) was stirred at rt for 4 h. The mixture was purified directly by flash chromatography to afford N-((5-chloro-lH-indazol-3- yl)methyl)-5-(4-((2-oxopiperazin-l -yl)methyl)benzyl)nicotinamide as a yellow solid (10 mg, 67%). LRMS (M+H+) m/z calculated 489.17, found 489.1. ¾ NMR (CD3OD, 400 MHz) δ 9.05 (s, 1 H), 8.78 (d, 2 H), 7.80 (s, 1 H), 7.40 (d, 1 H), 7.21 -7.26 (m, 5H), 4.83 (s, 2 H), 4.53 (s, 2 H), 4.17 (s, 2 H), 3.82 (s, 2 H), 3.41 -3.45 (m, 4 H). Example 110: Preparation of 5-(4-(acetamidomethyl)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000202_0001
To a solution of ethyl 5-bromonicotinate (5 g, 21.73 mmol, 1 eq) in 1,4-dioxane was added B2(Pin)2 (6.1 g, 23.91 mmol, 1.1 eq), KOAc (4.3 g, 43.46 mmol, 2 eq), and Pd(dppf)Cl2 (1.59 g, 2.17 mmol, 0.1 eq) under N2. The mixture was stirred at 90 °C for 3 h. Then to the mixture was added 4-(bromomethyl)benzonitrile (4.3 g, 21.73 mmol, 1 eq), water, and Na2CC>3 (4.6 g, 43.46 mmol, 2 eq). The mixture was stirred at 90 °C for 3 h, and then poured into water, extracted with EA. The organics was concentrated and purified via flash
chromatography (PE/EtOAc = 5/1) to afford ethyl 5-(4-cyanobenzyl)nicotinate as a yellow solid (3.5 g, 60 %).
Figure imgf000202_0002
To a solution of ethyl 5-(4-cyanobenzyl)nicotinate (2 g, 7.51 mmol , 1 eq) in MeOH was added Raney Ni. The mixture was stirred at rt overnight under ¾. The mixture was filtered, concentrated, and purified by fish chromatography (DCM/MeOH = 20/1) to afford methyl 5-(4-(aminomethyl)benzyl)nicotinate as a yellow solid (300 mg, 16 %).
Figure imgf000202_0003
To a solution of methyl 5-(4-(aminomethyl)benzyl)nicotinate (100 mg, 0.39 mmol, 1 eq) in DCM was added AC2O (60 mg, 0.59 mmol, 1.5 eq). The mixture was stirred at rt for 2 h, and concentrated. The residue was used for the next step without further purification.
Figure imgf000203_0001
To a solution of methyl 5-(4-(acetamidomethyl)benzyl)nicotinate (100 mg, 0.34 mmol, 1 eq) in THF/H2O was added LiOH (40 mg, 1.7 mmol, 5 eq), and the mixture was stirred at rt for 2 h. The mixture was acidified to pH 6 with cone. cation.
Figure imgf000203_0002
To a solution of 5-(4-(acetamidomethyl)benzyl)nicotinic acid (100 mg, 0.35 mmol, 1 eq) in DMF was added (3-chloro-lH-indol-5-yl)methanamine (63 mg, 0.35 mmol, 1 eq), HATU (160 mg, 0.42 mmol, 1.2 eq), and TEA (71 mg, 0.70 mmol, 2 eq). The mixture was stirred at rt overnight, then poured into water and extracted with EtOAc. The organics was concentrated and purified by prep-HPLC to afford 5-(4- (acetamidomethyl)benzyl)-N-((3-chloro-lH-indol-5-yl)methyl)nicotinamide as a white solid (5 mg, 3 %). LCMS (M+H+) m/z calculated 447.2, found 447.2. ¾ NMR (CD3OD, 400 MHz) δ 10.77-10.76 (m, 1 H) , 9.23-9.20 (m, 1 H), 8.87 (s, 1 H), 8.60-8.56 (m, 1 H), 8.13 (s , 1 H), 7.53 (s, 1 H), 7.39-7.22 (m, 7 H), 4.71- 4.68 (m, 1 H), 4.34 (d, 2 H), 4.08 (d, 2 H), 1.99 (s, 3 H). Example 111: Preparation of 5-(4-(2-amino-2-oxoethyl)benzyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide
Figure imgf000203_0003
To a solution of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(chloromethyl)benzyl)nicotinamide (120 mg, 0.28 mmol) in DMF (5 mL) were added KCN (18 mg, 0.28 mmol), and 18-crown-6 (7 mg, 0.28mmol). The mixture was stirred at 70 °C for 8 h. The mixture was cooled to rt. Water (20 mL) was added into the reaction mixture, then the mixture was extracted with EA (20 mL x 3). The organic phase was evaporated to dryness, and the residue was purified by flash chromatography to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4- (cyanomethyl)benzyl)nicotinamide as a yellow solid (60 mg, 50%).
Figure imgf000204_0001
To a solution of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(cyanomethyl)benzyl)nicotinamide (60 mg, 0.14 mmol, 1.0 eq) in DMSO (4 mL) were added 30% H202 (1.5 mL) and K2C03 (16 mg, 0.11 mmol). The mixture was stirred at 50 °C overnight. The mixture was purified directly by flash chromatography to afford 5-(4-(2- amino-2-oxoethyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)nicotinamide as a white solid (20 mg, 32%>). LRMS (M+H+) m/z calculated 434.13, found 434.1. ¾ NMR (CD3OD, 400 MHz) δ 9.29 (t, 1 H), 8.86 (s, 1 H), 8.62 (s, 1 H), 8.06 (s, 1 H), 7.91 (s, 1 H), 7.53 (d, 1 H), 7.41 (s, 1 H), 7.32 (m, 1 H), 7.17 (s, 4 H), 6.83 (s, 1 H), 4.78 (d, 2 H), 3.98 (s, 2 H), 3.30 (d, 2 H).
Example 112: Preparation of methyl 2-(4-((5-(((5-chloro-lH-indazol-3-yl)methyl)carbamoyl)pyridin-3- yl)methyl)phenyl) acetate
Figure imgf000204_0002
A solution of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(cyanomethyl)benzyl)nicotinamide (30 mg, 0.072 mmol, 1.0 eq) in MeOH (3 mL) and HC1/EA (3 N, 5 mL) was stirred at 50 °C for 20 h. Then the mixture was concentrated in vacuo to give an oil, which was purified by flash chromatography (MeCN/l hO = 1/2 to 19/1) to afford methyl 2-(4-((5-(((5-chloro-lH-indazol-3-yl)methyl)carbamoyl)pyridin-3-yl)methyl)phenyl)acetate as a white solid (27 mg, 83%). LRMS (M+H+) m/z calculated 449.1, found 449.1. ¾ NMR (DMSO-d6, 300 MHz) δ 13.12 (s, 1 H), 9.40 (t, 1 H), 8.95 (s, 1 H), 8.72 (s, 1 H), 8.24 (s, 1 H), 7.91 (s, 1 H), 7.53 (d, 1 H) , 7.33 (m, 1 H), 7.24-7.17 (m, 4 H), 4.79 (d, 2 H), 4.07 (s, 2 H), 3.62 (s, 2 H), 3.58 (s, 3 H).
Example 113: Preparation of N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-(ureidomethyl)benzyl)nicotinamide
Figure imgf000205_0001
chioro 1 H indoi 5 yl^methyl) 5 (4 (Ureidomethyl)benzy| njcotinamjc|e
Figure imgf000205_0002
To a solution of lH-indole-5-carbonitrile (13.9 g, 97.7 mmol, 1.0 eq) in MeOH (280 mL) was added B0C2O (42.6 g, 196 mmol, 2.0 eq) and Raney Ni (about 1.4 g). The mixture was hydrogenated at rt under 50 Psi overnight. The mixture was filtered and the filtrate was concentrated to dryness under reduced pressure. The residue was purified on silica gel column (PE/EA = 10/1 to 4/1) to afford tert-butyl ((lH-indol-5- yl)methyl)carbamate as a yellow oil (13.2 g, 55%).
Figure imgf000205_0003
To a solution of tert-butyl ((lH-indol-5-yl)methyl)carbamate (13.2 g, 53.6 mmol, 1.0 eq) in DCM (140 mL) was added portions NCS (7.13 g, 53.6 mmol, 1.0 eq) at 0 °C. The mixture was hydrogenated at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was purified on silica gel column (PE/EA = 10/1 to 4/1) to afford tert-butyl ((3-chloro-lH-indol-5-yl)methyl)carbamate as a white solid (9.7 g, 65%).
Figure imgf000205_0004
To a solution of tert-butyl ((3 -chioro- lH-indol-5-yl)methyl)carbamate (9.7 g, 34.6 mmol, 1.0 eq) in EA (40 mL) was added HCl/EA (40 mL, 80 mmol) at 0 °C. The mixture was stirred at rt overnight. The mixture was filtered. The filtered cake was dryness under reduced pressure to afford (3-chloro-lH-indol-5-yl)methanamine HC1 salt as a gray solid (6.0 g, 80%).
Figure imgf000206_0001
To a solution of methyl 5-(4-(aminomethyl)benzyl)nicotinate (160 mg, 0.62 mmol, 1.0 eq) in DCM (2 mL) were added Et3N (250 mg, 2.48 mmol, 4.0 eq) and isocyanatotrimethylsilane (214 mg, 1.86 mmol, 3.0 eq) at 0 °C. The mixture was stirred at rt overnight. The mixture was mixed with saturated NaHCC (10 mL). The mixture was concentrated to dryness under reduced pressure. The residue was purified via flash
chromatography to afford e solid (70 mg, 38%).
Figure imgf000206_0002
To a solution of methyl 5-(4-(ureidomethyl)benzyl)nicotinate (70 mg, 0.23 mmol, 1.0 eq) in MeOH (1 mL) and H2O (1 mL) was added NaOH (28 mg, 0.69 mmol, 3.0 eq). The mixture was heated at 60 °C for 2 h. The mixture was concentrated to remove MeOH, then diluted with ice-water (5 mL) and acidified with 1 N HCl to pH 2-3. The mixture was concentrated to dryness under reduced pressure. The residue was re-dissolved in MeOH (10 mL) and filtered. The filtrate was concentrated under reduced pressure to afford 5-(4- (ureidomethyl)benzyl)
Figure imgf000206_0003
To a solution of 5-(4-(ureidomethyl)benzyl)nicotinic acid (200 mg crude, 0.23 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (89 mg, 0.69 mmol, 3.0 eq) and HATU (105 mg, 0.28 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (3-chloro-lH-indol-5-yl)methanamine (55 mg, 0.25 mmol, 1.1 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was purified via flash chromatography to afford N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-(ureidomethyl)benzyl)nicotinamide as a white solid (6 mg, 6%). LRMS (M+H+) m/z caculated 448.1 , found 448.1. ¾ NMR (DMSO-</6, 400 MHz) δ 11.34 (brs, 1 H), 9.22 (s, 1 H), 8.89 (s, 1 H), 8.62 (s, 1 H), 8.05 (s, 1 H), 7.50-7.40 (m, 3 H), 7.38-7.15 (m, 5 H), 6.36 (s, 1 H), 5.49 (d, 2 H), 4.57 (d, 2 H), 4.12 (s, 2 H), 4.00 (d, 2 H). Example 114: Preparation of 6-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3- yl)methyl)quinoline-2-carboxamide
Figure imgf000207_0001
6"((5"(((3"ch|oro"i H"indor5"yl)methy|)Carbamoy|^pyridin"3'
yl met y|)qUjno|jne"2"car oxamjde
Figure imgf000207_0002
To a solution of 6-methylquinoline (10.0 g, 69.9 mmol, 1.0 eq) in HOAc (150 mL) was added 30% H202 (100 mL), The reaction mixture was heated to 70 °C and stirred overnight, then cooled, and water (100 mL) was added, then Na2S03 was added portion wise to quench excess H2O2 while cooling in ice bath. The reaction mixture was extracted with DCM and the organic phase was washed with water, brine, dried over anhydrous sodium sulfate, filtered and concentrated to dryness to afford 6-methylquinoline 1 -oxide as a brown oil (7.2 g, 64%).
Figure imgf000207_0003
To a solution of 6-methylquinoline 1 -oxide (7.2 g, 45.2 mmol, 1.0 eq) in trimethylsilycyanide (17.0 mL, 135.8 mmol, 3.0 eq) was added benzoyl chloride (15.6 mL, 135.8 mmol, 3.0 eq) while cooling in ice-water bath followed by triethylamine (18.9 mL, 135.8 mmol, 3.0 eq). The reaction was stirred for 1 h. The mixture was diluted with DCM, washed once with saturated aqueous NaHCC carefully, then washed with water, brine, dried over anhydrous sodium sulfate, and purified on silica gel column (PE/DCM/EA = 10/1/1) and then triturated from EtOH to afford 6-methylquinoline-2-carbonitrile as a yellow solid (6.0 g, 78%).
Figure imgf000207_0004
The mixture of 6-methylquinoline-2-carbonitrile (3.7 g, 22.0 mmol, 1.0 eq), NBS (3.9 g, 22.0 mmol, 1.0 eq) and AIBN (72 mg, 2 mol%>) in tetrachloride carbon (100 mL) was re fluxed for 3 h, then cooled and concentrated to dryness. The residue was triturated from DCM, filtered and dried to afford 6- (bromomethyl)quinoline-2-carbonitrile as a white solid (4.0 g, 74%>).
Figure imgf000207_0005
The mixture of ethyl 5-bromonictic acid (1.86 g, 8.1 mmol, 1.0 eq), BPDB (2.06 g, 8.1 mmool, 1.0 eq), potassium acetate (2.38 g, 24.3 mmol, 3.0 eq) and [1,1 '- bis(diphenylphosphino)ferrocene]dichloropalladium(II) (296 mg, 5 mol%) were mixed in 1,4-dioxane (100 mL). The mixture was degassed with nitrogen, heated to 85 °C and stirred for 16 h, then cooled to rt, and 6- (bromomethyl)quinoline-2-carbonitrile (2.0 g, 8.1 mmol, 1.0 eq), [Ι, - bis(diphenylphosphino)ferrocene]dichloropalladium(II) (296 mg, 5 mol%) and sodium carbonate (2.57 g, 24.3 mmol, 3.0 eq, dissolved in 30 mL of water) were added. The mixture was degassed, heated to 95 °C and stirred overnight. Cooled and the mixture was filtered through celite, water (100 mL) and DCM (100 mL) were added to the filtrate. The DCM layer was separated and washed with brine, dried over anhydrous sodium sulfate, and purified on silica gel column to afford ethyl 5-((2-cyanoquinolin-6-yl)methyl)nicotinate as a red solid (670 mg, 26%).
Figure imgf000208_0001
To the solution of ethyl 5-((2-cyanoquinolin-6-yl)methyl)nicotinate (670 mg, 2.1 mmol, 1.0 eq) in THF (6 mL) was added an aqueous solution of lithium hydroxide monohydrate (177 mg, 4.2 mmol, 2.0 eq) in water (3 mL). The reaction was stirred for 2 h, then acidified with 2 N HCl to pH 3. DCM (50 mL) and water (50 mL) were added, and the DCM layer was separated and washed with brine, dried over anhydrous sodium sulfate, and purified via flash chromatography to afford 5-((2-cyanoquinolin-6-yl)methyl)nicotinic acid as a brown solid (250
Figure imgf000208_0002
The mixture of 5-((2-cyanoquinolin-6-yl)methyl)nicotinic acid (250 mg, 0.86 mmol, 1.0 eq) and HATU (490 mg, 0.86 mmol, 1.5 eq) in DMF (5 mL) and DIEA (1 mL) was stirred for 1 h, and amine (187 mg, 0.86 mmol, 1.0 eq) was added. The reaction was stirred for 1 h, diluted with DCM, washed with water, and purified via flash chromatography to afford desired as a yellow solid (100 mg, 32%>).
Figure imgf000208_0003
To the solution of cyanide (10 mg, 0.22 mmol, 1.0 eq) in DMSO (3 mL) was added K2C03 (30 mg, 0.22 mmol, 1.0 eq), followed by a 30% H2O2 (3 mL). The reaction was stirred for 1 h, amine (187 mg, 0.86 mmol, 1.0 eq) was added. The reaction was stirred for 1 h, and quenched with water (5 mL). The precipitate was collected by filtration, rinsed with water, dried and triturated from MeOH then purified via flash
chromatography to afford desired as a white solid (52 mg, 50%>). LRMS (M+H)+ m/z calculated 470.1, found 470.1. 'H NMR (DMSO-ifc, 400 MHz) δ 11.32 (s, 1 H), 9.22 (t, 1 H), 8.92 (s, 1 H), 8.74 (s, 1 H), 8.47 (d, 1 H), 8.25 (s, 1 H), 8.15-8.10 (m, 2 H), 8.05 (d, 1 H), 7.93 (s, 1 H), 7.78-7.75 (m, 2 H), 7.49 (s, 1 H), 7.41 (t, 1 H), 7.36 (d, 1 H), 7.16 (d, 1 H), 4.56 (d, 2 H), 4.29 (s, 2 H).
Example 115: Preparation of methyl 6-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3- yl)methyl)quinoline-2-carboxylate
Figure imgf000209_0001
6-methylquinoline-2-carbonitrile (4.7 g, 23.3 mmol, 1.0 eq) was treated with a 25%> solution of HCl/MeOH and the mixture was heated to 50 °C and stirred for 4 h, then cooled to rt. After concentration to dryness, the residue was dissolved in DCM, washed with saturated aqueous sodium bicarbonate, water, brine and dried over anhydrous sodium sulfate, and purified via silica column (PE/EA = 10/1) to afford methyl 6- methylquinoline-2-carb
Figure imgf000209_0002
The mixture of methyl 6-methylquinoline-2-carboxylate (4.7 g, 23.3 mmol, 1.0 eq), NBS (4.1 g, 23.3 mmol, 1.0 eq) and AIBN (76 mg, 2 mol%>) in tetrachloride carbon (100 mL) was refluxed overnight, then cooled and concentrated to dryness. The residue was purified via silica column (PE/EA = 4/1) to afford methyl 6- (br
Figure imgf000209_0003
The mixture of benzyl 5-bromonictic acid (2.1 g, 7.1 mmol, 1.0 eq), 4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl-4',4',5',5'-tetramethyl-l,3,2-dioxaborolane (1.8 g, 7.1 mmol, 1.0 eq), potassium acetate (2.1 g, 21.3 mmol, 3.0 eq) and (l, -bis(diphenylphosphino)ferrocene)palladium(II) dichloride (105 mg, 2 mol%) in 1,4-dioxane (100 mL) was degassed and stirred at 80 °C overnight, then cooled to rt, methyl 6-(bromomethyl)quinoline-2- carboxylate (2.0 g, 7.1 mmol, 1.0 eq), (l, -bis(diphenylphosphino)ferrocene)palladium(II) dichloride (105 mg, 2 mol%) and an aqueous sodium carbonate (2.3 g, 21.3 mmol, 3.0 eq) in water (25 mL) were added successively. The mixture was degassed, heated to 60 °C and stirred overnight. After cooling, the mixture was filtered through celite, and water (100 mL) and DCM (100 mL) were added to the filtrate. The DCM layer was separated, washed with brine, dried over anhydrous sodium sulfate, and purified on silica gel column to afford methyl 6-((5-((benzyloxy)carbonyl)pyridin-3-yl)methyl)quino line -2 -carboxylate (460 mg, 15%).
Figure imgf000210_0001
The mixture of methyl 6-((5-((benzyloxy)carbonyl)pyridin-3-yl)methyl)quinoline-2-carboxylate (350 mg, 0.84 mmol, 1.0 eq) and Pd/C (10%, 50 mg) in MeOH (15 mL) was hydrogenated with ¾ balloon overnight. Then it was filtered through celite, rinsed with DCM/MeOH (1/1, 100 mL), and the filtrate was concentrated to dryness to afford 5-((2-(methoxycarbonyl)quinolin-6-yl)methyl)nicotinic acid as a yellow solid (235 mg, 86%).
Figure imgf000210_0002
The mixture of 5-((2-(methoxycarbonyl)quinolin-6-yl)methyl)nicotinic acid (150 mg, 0.46 mmol, 1.0 eq), HATU (266 mg, 0.7 mmol, 1.5 eq) in DCM (10 mL) and DIEA (1 mL) was stirred for 15 min, then (3-chloro- lH-indol-5-yl)methanamine hydrochloride (101 mg, 0.46 mmol, 1.0 eq) was added. The reaction was stirred for 2 h, quenched with water. The DCM layer was separated, washed with brine, dried over anhydrous sodium sulfate, and purified on silica gel column to afford methyl 6-((5-(((3-chloro-lH-indol-5- yl)methyl)carbamoyl)pyridin-3-yl)methyl)quinoline-2-carboxylate as a white solid (100 mg, 45%>). ¾ NMR (DMSO-d6, 300 MHz) δ 11.31 (s, 1 H), 9.22 (t, 1 H), 8.92 (s, 1 H), 8.74 (s, 1 H), 8.52 (d, 1 H), 8.16-8.08 (m, 3 H), 7.95 (s, 1 H), 7.80 (d, 1 H), 7.49 (s, 1 H), 7.41-7.34 (m, 2 H), 7.16 (d, 2 H), 4.29 (s, 2 H), 3.94 (s, 3 H).
Example 116: Preparation of N-((3-chloro-lH-indol-5-yl)methyl)-5-((2-methoxyquinazolin-6- yl)methyl)nicotinamide
Figure imgf000211_0001
Λ "((3"θ |θΓθ"ΐ Η ηάθΓ5 ΐ) 6Ι γΙ)"5"((2^6ΐ θΧγςυ ίη3Ζθ|ίη γ^βΐ γΙ)ηί∞ΐίη3ΐτΐί0|β
Figure imgf000211_0002
The mixture of 6-bromo-2-chloroquinazoline (2.435 g, 10 mmol, 1 eq) and C]¾ONa (2.75 mg, 51 mmol, 5.1 eq) in MeOH (60 mL) was stirred at 70 °C overnight. After cooling to rt, the solid was filtered, and washed with water to afford the first crop of the product. The filtrate was concentrated and the residue was partitioned between water (50 mL) and DCM (50 mL). The aqueous layer was extracted with DCM (50 mL X 2). The combined organic layers were dried over anhydrous Na2S04 and concentrated. The residue was combined with the first crop of the product and purified on silica gel column (EA/PE/DCM = 1/6/1, v/v/v) to afford 6- bromo-2-methoxyquinazoline as a light yellow solid (2.30 g, 96.2%).
Figure imgf000211_0003
A mixture of 6-bromo-2-methoxyquinazoline (120 mg, 0.50 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'- bi(l,3,2-dioxaborolane) (153 mg, 0.6 mmol, 1.2 eq), Pd(dppf)Cl2.CH2Ci2 (43 mg, 0.05 mmol, 0.1 eq), KOAc (148 mg, 1.5 mmol, 3 eq) in 1,4-dioxane (5 mL) under N2 was stirred at 100 °C overnight and concentrated. The residue was purified on silica gel column (EA PE = 1/6, v/v) to afford 2-methoxy-6-(4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl)quinazoline as a white solid (150 mg crude, quant)
Figure imgf000211_0004
To the solution of methyl 5-(hydroxymethyl)nicotinate (501 mg, 3 mmol, 1 eq) in DCM (10 mL) was added SOCb (1 mL). The mixture was stirred at rt overnight and concentrated to afford methyl 5- (chloromethyl)nicotinate hydrochloride as a white solid (660 mg, 99 %).
Figure imgf000211_0005
A mixture of methyl 5-(chloromethyl)nicotinate hydrochloride (1.19 g, 5.36 mmol, 1.0 eq), 2-methoxy-6- (4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)quinazoline (2.2 g, 7 mmol, 1.3 eq), Pd(dppf)Cl2.CH2Cl2 (590 mg, 0.72 mmol, 0.13 eq), Na2C03 (3.0 g, 28.3 mmol, 5.3 eq) in l,4-dioxane/H20 (45 mL/4.5 mL) under N2 was stirred at 90 °C for 6 h and then filtered. The filtrate was concentrated and the residue was purified via silica column (EA/PE =1/4-1/1, v/v) to afford methyl 5-((2-methoxyquinazolin-6-yl)methyl)nicotinate as a white solid (1.62 g, 97.8%).
Figure imgf000212_0001
To the solution of methyl 5-((2-methoxyquinazolin-6-yl)methyl)nicotinate (1.62 g, 5.24 mmol, 1 eq) in THF/MeOH (10 mL/5 mL) was added NaOH aqueous solution (I N, 10 mL). The mixture was stirred at rt overnight and concentrated. The residue was acidified with HCl to pH 2. Filtered and the solid was collected to afford 5-(
Figure imgf000212_0002
A mixture of 5-((2-methoxyquinazolin-6-yl)methyl)nicotinic acid (64 mg, 0.217 mmol, 1.0 eq), (3-chloro-lH- indol-5-yl)methanamine HCl salt (70 mg, 0.32 mmol, 1.5 eq), HATU (114 mg, 0.30 mmol, 1.4 eq) and DIPEA (1 mL) in DMF (3 mL) was stirred at rt overnight. The mixture was filtered and the filtrate was purified via prep-HPLC to afford N-((3-chloro-lH-indol-5-yl)methyl)-5-((2-methoxyquinazolin-6- yl)methyl)nicotinamide as a yellow solid (40 mg, 40.4%). LRMS (M+H+) m/z calculated 458.1, found 458.1. ¾ NMR (CD3OD, 400 MHz) δ 9.24 (s, 1 H), 8.89 (d, 1 H), 8.66 (d, 1 H), 8.17 (s, 1 H), 7.86 (s, 1 H), 7.81 (dd, 1 H), 7.76 (d, 1 H), 7.51 (s, 1 H), 7.34 (d, 1 H), 7.24 (s, 1 H), 7.20 (dd, 1 H), 4.67 (s, 2 H), 4.28 (s, 2 H), 4.10 (s, 3 H).
Example 117: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-((2-methoxyquinazolin-6- yl)methyl)nicotinamide
Figure imgf000213_0001
A mixture of 5-((2-methoxyquinazolin-6-yl)methyl)nicotinic acid (295 mg, 1 mmol, 1.0 eq), (5-chloro-l- (tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (319 mg, 1.2 mmol, 1.2 eq), HATU (456 mg, 1.2 mmol, 1.2 eq) and DIPEA (1 mL) in DMF (5 mL) was stirred at rt overnight. The mixture was filtered and the solid was collected to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-((2- methoxyquinazolin-6-yl)methyl)nicotinamide as a white solid (335 mg, 61.8%).
Figure imgf000213_0002
To the solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-((2- methoxyquinazolin-6-yl)methyl)nicotinamide (50 mg, 0.09 mmol, 1 eq) in DCM (3 mL) was added TFA (0.5 mL). The mixture was stirred at rt overnight and neutralized with NF VMeOH. The mixture was purified via prep-MPLC to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-((2-methoxyquinazolin-6- yl)methyl)nicotinamide as a white solid (10 mg, 23.8 %). LRMS (M+H+) m/z calculated 459.1, found 459.1. ¾ NMR (DMSO-de, 400 MHz) δ 9.39 (s, 1 H), 9.31 (s, 1 H), 8.91 (s, 1 H), 8.72 (s, 1 H), 8.12 (s, 1 H), 7.91 (d, 2 H), 7.85 (d, 1 H), 7.75 (d, 1 H), 7.53 (d, 1 H), 7.33 (d, 1 H), 4.78 (d, 2 H), 4.24 (s, 2 H), 4.01 (s, 3 H).
Example 118: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxooxazolidin-3- yl)benzyl)nicotinamide
Figure imgf000214_0001
A mixture of 5-(4-bromobenzyl)-N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)nicotinamide (108 mg, 0.20 mmol, 1.0 eq), oxazolidin-2-one (56 mg, 0.64 mmol, 3.2 eq), Pd2(dba)3 (22 mg, 0.024 mmol, 0.12 eq), Xantphos (26 mg, 0.044 mmol, 0.22 eq), K2C03 (108 mg, 0.78 mmol, 3.9 eq) in toluene (5 mL) under N2 was stirred at 100 °C overnight and concentrated. The residue was purified via prep-MPLC to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-(2- oxooxazolidin- -yl)benzyl)nicotinamide as a white solid (42 mg, 38.5%)
Figure imgf000214_0002
The mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-(2-oxooxazolidin-3- yl)benzyl)nicotinamide (42 mg, 0.077 mmol, 1 eq) in HCPEA (5 N, 4 mL) was stirred at rt overnight. The precipitate was collected by suction to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxooxazolidin-3- yl)benzyl)nicotinamide hydrochloride as a white solid (23 mg, 60.5%). LRMS (M+H+) m/z calculated 462.1, found 462.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.31 (s, 1 H), 9.49 (t, 1 H), 9.01 (d, 1 H), 8.78 (d, 1 H), 8.38 (s, 1 H), 7.92 (d, 1 H), 7.55-7.49 (m, 3 H), 7.35-7.30 (m, 3 H), 4.80 (d, 2 H), 4.41 (t, 2 H), 4.09 (s, 2 H), 4.02 (t, 2 H).
Example 119: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxoimidazolidin-l - yl)benzyl)nicotinamide
Figure imgf000215_0001
A mixture of 5-(4-bromobenzyl)-N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)nicotinamide (108 mg, 0.20 mmol, 1.0 eq), imidazolidin-2-one (86 mg, 1.0 mmol, 5 eq), Pd2(dba)3 (24 mg, 0.024 mmol, 0.12 eq), Xantphos (31 mg, 0.054 mmol, 0.27 eq), K2C03 (138 mg, 1.0 mmol, 5 eq) in toluene (5 mL) under N2 was stirred at 100 °C overnight and concentrated. The residue was purified via prep- MPLC to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-(2- oxoimidazolid -l-yl)benzyl)nicotinamide as a white solid (26 mg, 23.9%)
Figure imgf000215_0002
The mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-(2-oxoimidazolidin- l-yl)benzyl)nicotinamide (26 mg, 0.048 mmol, 1 eq) in HC1/EA (5 N, 2 mL) and MeOH (2 mL) was stirred at rt overnight. The volatiles were concentrated to afford N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2- oxoimidazolidin-l -yl)benzyl)nicotinamide hydrochloride as a yellow solid (20 mg, 84.3%). LRMS (M+H+) m/z calculated 461.1, found 461.1. 'H NMR (CD3OD, 400 MHz) δ 9.16 (s, 1 H), 8.89 (s, 1 H), 8.87 (s, 1 H), 7.91 (d, 1 H), 7.54 (d, 2 H), 7.52 (d, 1 H), 7.39 (dd, 1 H), 7.29 (d, 2 H), 4.98 (s, 2 H), 4.27 (s, 2 H), 3.96 (t, 2 H), 3.56 (t, 2 H).
Example 120: Preparation of N-((6-(aminomethyl)pyridin-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)nicotinamide
Figure imgf000216_0001
A mixture of 5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinic acid (70 mg, 0.21 mmol, 1.0 eq), 5-(aminomethyl)picolinonitrile HC1 salt (45 mg, 0.21 mmol, 1.0 eq), HATU (83 mg, 0.21 mmol, 1.0 eq) and DIEA (0.5 mL) in DCM (10 mL) was stirred at rt for 3 h. The mixture was evaporated under reduced pressure to give an oil, which was purified by flash chromatography (MeCN/l hO = 1/4 to 2/1) to afford N- ((6-cyanopyridin-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide as white solid (82 mg, 86%).
Figure imgf000216_0002
N-((6-Cyanopyridin-3-yl)methyl)-5-((6-((2-oxopyridin-l (2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide (80 mg, 0.18 mmol, 1.0 eq) and (Boc)20 (120 mg, 0.55 mmol, 3.0 eq) was dissolved in 10 mL of methanol, combined with 50 mg Raney nickel and hydrogenated at rt for 2 h at a pressure of 1 atm. Then the catalyst was filtered off and the filtrate was concentrated under reduced pressure to give tert-butyl ((5-((5-((6-((2- oxopyridin- 1 (2H)-yl)methyl)pyridin-3 -yl)methyl)nicotinamido)methyl)pyridin-2-yl)methyl)carbamate (46 mg, 46%).
Figure imgf000217_0001
A solution of tert-butyl ((5-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamido)methyl)pyridin-2-yl)methyl)carbamate (46 mg, 0.085 mmol, 1.0 eq) in MeOH (3 mL) and HCl/MeOH (3 N, 2 mL) was stirred at rt for 2 h. Then the mixture was concentrated. The residue was recrystallized with MeOH/EtOAc to afford N-((6-(aminomethyl)pyridin-3-yl)methyl)-5-((6-((2-oxopyridin- l(2H)-yl)methyl)pyridin-3-yl)methyl)nicotinamide as a white solid (29 mg, 72%). LRMS (M+H+) m/z calculated 441.2, found 441.2. ¾ NMR (DMSO-ifc, 300 MHz) δ 9.87 (t, 1 H), 9.14 (s, 1 H), 8.93 (d, 1 H), 8.72 (s, 1 H), 8.64 (s, 1 H), 8.53 (br, 3 H), 8.06 (d, 1 H), 7.92 (dd, 1 H) , 7.86 (dd, 1 H), 7.55 (d, 1 H), 7.47 (m, 1 H), 7.37 (d, 1 H), 6.39 (d, 1 H), 6.31-6.27 (m, 1 H), 5.27 (s, 2 H), 4.54 (d, 2 H), 4.24 (s, 2 H), 4.18 (m, 2 H).
Example 121: Preparation of methyl 3-(4-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3- yl)methyl)phenyl)propanoate
Figure imgf000217_0002
To a solution of tert-butyl 5-bromonicotinate (5.0 g, 19.4 mmol, 1.0 eq) in 1,4-dioxane (200 mL) were added KOAc (5.7 g, 58.2 mmol, 3.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2-dioxaborolane) (4.93 g, 19.4 mmol, 1.0 eq) and Pd(dppf)Cl2 (355 mg, 0.49 mmol, 0.03 eq). The mixture was heated at 85 °C overnight under N2, and then the mixture was cooled to rt. To the mixture were added methyl 4-(bromomethyl)benzoate (4.44 g, 19.4 mmol, 1.0 eq), Pd(dppf)Cl2 (355 mg, 0.49 mmol, 0.03 eq) and Na2C03 (6.17 g, 58.2 mmol, 3.0 eq) in water (40 mL). The mixture was heated to 80 °C overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (100 mL) and extracted with EA (100 mL x 3). The combined organic layers were washed with brine (50 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified on silica gel column (PE/EA = 10/1 to 4/1) to afford tert-butyl 5-(4- (methoxycarbonyl)benzyl)nicotinate as a yellow solid (3.8 g, 60%).
Figure imgf000218_0001
To a solution of tert-butyl 5-(4-(methoxycarbonyl)benzyl)nicotinate (3.8 g, 11.6 mmol, 1.0 eq) in THF (80 mL) was added L1AIH4 (441 mg, 11.6 mmol, 1.0 eq) at -78 °C under N2. The mixture was stirred at rt overnight. The mixture was added Na2SO4.10H2O to work up at -78 °C. The mixture was stirred warm to rt. The mixture was filtered. The filter cake was washed with EA (30 mL x 2). The filtrate was concentrated. The residue was purified on silica gel column (PE/EA = 10/1 to 3/1) to afford tert-butyl 5-(4- (hydroxymethyl)benzyl)nicotinate as a yellow solid (2.0 g, 58%).
Figure imgf000218_0002
To a solution of tert-butyl 5-(4-(hydroxymethyl)benzyl)nicotinate (700 mg, 2.34 mmol, 1.0 eq) in DCM (7 mL) were added MnC (2.0 g, 23.4 mmol, 10.0 eq). The suspension was stirred at rt overnight. The mixture was filtered on a short silica gel column, and washed with (DCM/MeOH = 20 mL/20 mL). The filtrate was concentrated to afford tert-butyl 5-(4-formylbenzyl)nicotinate as a white solid (700 mg crude, quant).
Figure imgf000218_0003
To a solution of methyl 2-(dimethoxyphosphoryl)acetate (450 mg, 2.47 mmol, 1.05 eq) in THF (7 mL) was added dropwise n-BuLi (1.1 mL, 2.5 M, 2.7 mmoL, 1.15 eq) at -78 °C under N2. The mixture was stirred at - 78 °C for 30 min. tert-butyl 5-(4-formylbenzyl)nicotinate (700 mg, 2.36 mmol, 1.0 eq) in THF (5 mL) was added dropwise into the mixture. The mixture was stirred at rt overnight. The mixture was work up with water (2 mL) at 0 °C, saturated NaHCC (5 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2SC>4 and concentrated to afford (E)-tert-butyl 5-(4- (3-methoxy-3-oxoprop-l-en-l -yl)benzyl)nicotinate as a white solid (880 mg, 95%).
Figure imgf000219_0001
To a solution of (E)-tert-butyl 5-(4-(3-methoxy-3-oxoprop-l-en-l -yl)benzyl)nicotinate (880 mg, 2.49 mmol, 1.0 eq) in EA (8 mL) and MeOH (4 mL) was added Pd/C (170 mg, 10%Pd). The mixture was hydrogenated at rt for 4 h. The mixture was filtered and the filtered cake was washed with DCM (10 mL) and MeOH (10 mL). The filtrate was concentrated to dryness under reduced pressure to afford tert-butyl 5-(4-(3-methoxy-3- oxopropyl)benzyl)nicotinate as a white solid (820 mg, 93%).
Figure imgf000219_0002
To a solution of tert-butyl 5-(4-(3-methoxy-3-oxopropyl)benzyl)nicotinate (820 mg, 2.31 mmol, 1.0 eq) in TFA (4 mL) and DCM (8 mL). The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure to afford 5-(4-(3-methoxy-3-oxopropyl)benzyl)nicotinic acid as a brown oil (1.5 g crude, quant).
Figure imgf000219_0003
To a solution of 5-(4-(3-methoxy-3-oxopropyl)benzyl)nicotinic acid (750 mg crude, 1.16 mmol, 1.0 eq) in DMF (4 mL) were added DIPEA (1.5 g, 11.6 mmol, 10.0 eq) and HATU (529 mg, 1.39 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (3-chloro-lH-indol-5-yl)methanamine (251 mg, 1.16 mmol, 1.0 eq) was then added and the mixture was stirred at rt for 2 h under N2. The mixture was mixed with water (10 mL) and extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford methyl 3-(4-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-yl)methyl)phenyl)propanoate as an off-white solid (400 mg, 75%). LRMS (M+H+) m/z caculated 462.1 , found 462.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.32 (brs, 1 H), 9.20 (t, 1 H), 8.88 (d, 1 H), 8.62 (d, 1 H), 8.05 (d, 1 H), 7.50 (d, 1 H), 7.42 (s, 1 H), 7.37 (d, 1 H), 7.20-7.10 (m, 5 H), 4.56 (d, 2 H), 3.98 (s, 2 H), 3.55 (s, 3 H), 2.80 (t, 2 H), 2.58 (t, 2 H). Example 122: Preparation of 5-(4-(3-amino-3-oxopropyl)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000220_0001
5"(4"(3"amino"3"oxopropy|)benzy|^"((3"ch|oro"i H^ndo 5"y|)methyl)nicotinamide
Figure imgf000220_0002
To a solution of 3-(4-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-yl)methyl)phenyl)propanoic acid (280 mg crude, 0.38 mmol, 1.0 eq) in DMF (3 mL) were added DIPEA (245 mg, 1.9 mmol, 5.0 eq) and HATU (173 mg, 0.46 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, NH4C1 (102 mg, 1.9 mmol, 5.0 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was purified via flash chromatography to afford 5-(4-(3-amino-3-oxopropyl)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide as a white solid (75 mg, 44%). LRMS (Μ+ΡΓ) m/z caculated 447.1 , found 447.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.33 (brs, 1 H), 9.20 (t, 1 H), 8.88 (d, 1 H), 8.62 (d, 1 H), 8.05 (s, 1 H), 7.50 (d, 1 H), 7.42 (s, 1 H), 7.37 (d, 1 H), 7.26 (brs, 1 H), 7.20-7.10 (m, 5 H), 6.74 (brs, 1 H), 4.56 (d, 2 H), 3.98 (s, 2 H), 2.74 (t, 2 H), 2.30 (t, 2 H). Example 123: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyrrolidin-l - yl)methyl)benzyl)nicotinamide
Figure imgf000220_0003
N ((5 chioro 1 H indazoi 3 yl^methyl) 5 (4 ((2 oxopyrro|idin 1 yi^methy^benzyi^njcotinamjde
Figure imgf000221_0001
To a solution of tert-butyl 5-(4-(hydroxymethyl)benzyl)nicotinate (300 mg, 1.0 mmol, 1.0 eq) in DCM (3 mL) were added Et3N (303 mg, 3.0 mmol, 3.0 eq) and MsCl (127 mg, 1.1 mmol, 1.1 eq) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with saturated NaHC03 (5 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford tert-butyl 5-(4-(chloromethyl)benzyl)nicotinate as a white solid (140 mg, 38%).
Figure imgf000221_0002
To a solution of pyrrolidin-2-one (224 mg, 2.64 mmol, 6.0 eq) in THF (4 mL) were added NaH (53 mg, 1.32 mmol, 3.0 eq) at 0 °C under N2. The suspension was stirred at 0 °C for 15 min, then tert-butyl 5-(4- (chloromethyl)benzyl)nicotinate (140 mg, 0.44 mmol, 1.0 eq) was added into the mixture. The mixture was stirred at rt overnight. The reaction mixture was poured into ice-water (5 mL), acidified with 1 N HCl to pH 2- 3, and concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford 5-(4-((2- .
Figure imgf000221_0003
To a solution of 5-(4-((2-oxopyrrolidin-l-yl)methyl)benzyl)nicotinic acid (50 mg, 0.16 mmol, 1.0 eq) in DMF (2 mL) were added DIPEA (103 mg, 0.8 mmol, 5.0 eq) and HATU (73 mg, 0.19 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methanamine (43 mg, 0.16 mmol, 1.0 eq) was then added and the mixture was stirred at rt for 2 h under N2. The mixture was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH- indazol-3-yl)methyl)-5-(4-((2-oxopyrrolidin-l -yl)methyl)benzyl)nicotinamide as a white solid (70 mg, 79%).
Figure imgf000222_0001
To a solution of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyrrolidin- l -yl)methyl)benzyl)nicotinamide (70 mg, 0.13 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/MeOH (2 mL) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (5 mL), neutralized with saturated NaHC03 (10 mL) and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3- yl)methyl)-5-(4-((2-oxopyrrolidin-l -yl)methyl)benzyl)nicotinamide as a white solid (34 mg, 58%). LRMS (M+H+) m/z caculated 474.1 , found 474.2. ¾ NMR (DMSO-i e, 400 MHz) δ 13.07 (brs, 1 H), 9.29 (t, 1 H), 8.87 (d, 1 H), 8.63 (d, 1 H), 8.06 (s, 1 H), 7.90 (d, 1 H), 7.53 (d, 1 H), 7.33 (dd, 1 H), 7.23 (d, 2 H), 7.13 (d, 2 H), 4.78 (d, 2 H), 4.30 (s, 2 H), 4.00 (s, 2 H), 3.18 (t, 2 H), 2.47 (t, 2 H), 2.00-1.80 (m, 2 H).
Example 124: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxoimidazolidin-l - yl)methyl)benzyl)nicotinamide
Figure imgf000222_0002
A solution of tert-butyl 5-(4-(hydroxymethyl)benzyl)nicotinate (1.7 g, 5.69 mmol, 1.0 eq) in TFA (4 mL) and DCM (20 mL) was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was purified via flash chromatography to afford 5-(4-(hydroxymethyl)benzyl)nicotinic acid as a white solid (1.18 g, 86%).
Figure imgf000223_0001
To a solution of 5-(4-(hydroxymethyl)benzyl)nicotinic acid (1.18 g, 4.86 mmol, 1.0 eq) in DMF (10 mL) were added DIPEA (3.13 g, 24.3 mmol, 5.0 eq) and HATU (2.22 g, 5.83 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l-(tetrahydro-2H-pyran-2-yl)-l H-indazol-3 -yl)methanamine (1.29 g, 4.86 mmol, 1.0 eq) was then added and the mixture was stirred at rt for 2 h under N2. The mixture was purified via flash chromatography to afford N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)-5-(4-
Figure imgf000223_0002
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4- (hydroxymethyl)benzyl)nicotinamide (1.9 g, 3.87 mmol, 1.0 eq) in DCM (38 mL) were added Et3N (1.17 g, 11.6 mmol, 3.0 eq) and SOCh (4.6 g, 38.7 mmol, 10.0 eq) at 0 °C. The mixture was stirred at rt for 2 h. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with saturated NaHCCb (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-5-(4-(chloromethyl)benzyl)nicotinamide as a white solid
Figure imgf000223_0003
To a solution of imidazolidin-2-one (101 mg, 1.18 mmol, 6.0 eq) in DMF (2 mL) were added NaH (24 mg, 60%) in oil, 0.59 mmol, 3.0 eq) at 0 °C under N2. The suspension was stirred at 0 °C for 30 min. Then N-((5- chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3 -yl)methyl)-5-(4-(chloromethyl)benzyl)nicotinamide (100 mg, 0.2 mmol, 1.0 eq) was added into the mixture. The mixture was stirred at rt overnight. The reaction mixture was poured into ice-water (5 mL), extracted with EA (10 mL X 3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2- oxoimidazolidin-l -yl)methyl)benzyl)nicotinamide as a white solid (20 mg, 18%).
Figure imgf000224_0001
To a solution of N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2- oxoimidazolidin-l -yl)methyl)benzyl)nicotinamide (20 mg, 0.036 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/MeOH (2 mL) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (5 mL), neutralized with saturated NaHCCb (5 mL) and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3- yl)methyl)-5-(4-((2-oxoimidazolidin-l -yl)methyl)benzyl)nicotinamide as a white solid (17 mg, quant). LRMS (M+H+) m/z caculated 475.1 , found 475.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.09 (brs, 1 H), 9.30 (t, 1 H), 8.87 (d, 1 H), 8.63 (d, 1 H), 8.07 (s, 1 H), 7.91 (d, 1 H), 7.53 (d, 1 H), 7.33 (d, 1 H), 7.25-7.15 (m, 4 H), 6.39 (brs, 1 H), 4.78 (d, 2 H), 4.17 (s, 2 H), 4.00 (s, 2 H), 3.18-3.15 (m, 4 H).
Example 125: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxooxazolidin-3- yl)methyl)benzyl)nicotinamide
Figure imgf000224_0002
To a solution of oxazolidin-2-one (102 mg, 1.18 mmol, 6.0 eq) in DMF (2 mL) were added NaH (24 mg, 60% in oil, 0.59 mmol, 3.0 eq) at 0 °C under N2. The suspension was stirred at 0 °C for 30 min. Then N-((5-chloro- l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-(chloromethyl)benzyl)nicotinamide (100 mg, 0.2 mmol, 1.0 eq) was added into the mixture. The mixture was stirred at rt overnight. The reaction mixture was poured into ice-water (5 mL), extracted with EA (10 mL x 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2- oxooxazolidin-3-yl)methyl)benzyl)nicotinamide as a white solid (60 mg, 55%).
Figure imgf000225_0001
To a solution of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((2-oxooxazolidin- 3-yl)methyl)benzyl)nicotinamide (60 mg, 0.11 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/MeOH (2 mL) at 0 °C. The mixture was stirred at rt overnight. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (5 mL), neutralized with saturated NaHCC (5 mL) and concentrated. The residue was purified via flash chromatography to afford N-((5-chloro-lH-indazol-3- yl)methyl)-5-(4-((2-oxooxazolidin-3-yl)methyl)benzyl)nicotinamide as a white solid (47 mg, 92%). LRMS (M+H+) m/z caculated 476.1, found 476.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.09 (brs, 1 H), 9.30 (t, 1 H), 8.88 (d, 1 H), 8.64 (d, 1 H), 8.07 (s, 1 H), 7.91 (d, 1 H), 7.55-7.50 (m, 1 H), 7.40-7.20 (m, 5 H), 4.78 (d, 2 H), 4.30-4.20 (m, 4 H), 4.00 (d, 2 H), 3.37 (s, 2 H). Example 126: Preparation of 5-(4-(2-amino-2-oxoethoxy)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000225_0002
A mixture of 2-(4-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-yl)methyl)phenoxy)acetic acid (75 mg, 0.17 mmol, 1.0 eq), NH4C1 (11 mg, 0.2 mmol, 1.2 eq), HATU (97 mg, 0.26 mmol, 1.5 eq) and DIEA (66 mg, 0.51 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt for 2 h. The mixture was purified by flash chromatography to afford 5-(4-(2-amino-2-oxoethoxy)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide as a white solid (10.8 mg, 14%).LRMS (M+H+) m/z calculated 449.14, found 449.1. 'H NMR (DMSO-i/6, 400 MHz) δ 11.33 (s, 1 H), 9.20 (t, 1 H), 8.87 (s, 1 H), 8.61 (s, 1 H), 8.04 (s, 1 H), 7.50 (s, 1 H), 7.47 (s, 1 H), 7.42 (s, 1 H), 7.38-7.36 (m, 2 H), 7.20-7.16 (m, 3 H), 6.87 (d, 2 H), 4.56 (d, 2 H), 4.37 (s, 2 H), 3.96 (s, 2 H).
Example 127: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((N- methylacetamido)methyl)benzyl)nicotinamide
Figure imgf000226_0001
N ((5 chioro 1 H jndazoi 3 yi^methyl) 5 (4 ((/V methyiacetamjdo^methy^benzyi^njcotinamjde
Figure imgf000226_0002
A mixture of l-(4-bromophenyl)-N-methylmethanamine (2 g, 10 mmol, 1.0 eq), TEA (3 g, 30 mmol, 3 eq) was cooled to 0 °C. Then acetyl chloride (942 mg, 12 mmol, 1.2 eq) in DCM (20 mL) was added. The mixture was stirred at rt for 1 h. The mixture was washed with brine,dried over Na2SO i, filtered and concentrated. The residue was purified on silica gel column (PE/EtOAc = 5/1) to give N-(4-bromobenzyl)-N-methylacetamide as a yellow oil (1.6 g, 67%).
Figure imgf000226_0003
The mixture of N-(4-bromobenzyl)-N-methylacetamide (872 mg, 3.6 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'- octamethyl-2,2'-bi(l,3,2-dioxaborolane) (914 mg, 3.6 mmol, 1.0 eq), Pd(dppf)Cl2 (131 mg, 5%) and KOAc (1 g, 10.8 mmol, 3.0 eq) in dioxane (20 mL) was degassed with N2 and stirred at 85 °C overnight. Then the mixture was concentrated. The residue was diluted with EtOAc (100 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified via flash chromatography (PE/EtOAc = 5/1) to afford N- methyl-N-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)benzyl)acetamide as a yellow solid (750 mg, 75%).
Figure imgf000227_0001
The mixture of methyl 5-(chloromethyl)nicotinate HC1 salt (577 mg, 2.6 mmol, 1.0 eq), N-methyl-N-(4- (4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)benzyl)acetamide (750 mg, 2.6 mmol, 1.0 eq), Pd(dppf)Ci2 (95 mg, 0.05 eq) and Na2CC (827 mg, 7.8 mmol, 3.0 eq, solution in 10 mL of water) in 1,4-dioxane (20 mL) was degassed with N2 and stirred at 95 °C overnight. Then the mixture was cooled and concentrated. The residue was dissolved in EtOAc (150 mL), washed with water, brine and dried over anhydrous sodium sulfate, filtered and purified on silica gel column (PE/EtOAc = 1/2) to afford methyl 5-(4-((N- methylacetamido)me
Figure imgf000227_0002
To a solution of methyl 5-(4-((N-methylacetamido)methyl)benzyl)nicotinate (300 mg, 0.96 mmol, 1.0 eq) in MeOH (3 mL) and H2O (3 mL) was added NaOH (115 mg, 2.88 mmol, 3.0 eq). The mixture was stirred at rt for 1 h. Then the organic solvents were evaporated. The residue was neutralized with 1 N HC1. The obtained mixture was concentrated to afford 5-(4-((N-methylacetamido)methyl)benzyl)nicotinic acid as a yellow solid (454
Figure imgf000227_0003
A mixture of 5-(4-((N-methylacetamido)methyl)benzyl)nicotinic acid (277 mg, crude, 0.48 mmol, 1.0 eq), (5- chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (127 mg, 0.48 mmol, 1.0 eq), HATU (274 mg, 0.72 mmol,1.5 eq) and DIEA (185 mg, 1.44 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt for 2 h. The mixture was purified directly by flash chromatography to afford N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)- lH-indazol-3-yl)methyl)-5-(4-((N-methylacetamido)methyl)benzyl)nicotinamide as a yellow oil (158 mg, 60%).
Figure imgf000228_0001
A mixture of N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methyl)-5-(4-((N- methylacetamido)methyl)benzyl)nicotinamide (158 mg, 0.29 mmol, 1.0 eq) in HCl/MeOH (25% w/w, 10 mL) was stirred at rt overnight. The mixture was purified directly by flash chromatography to afford N-((5-chloro- lH-indazol-3-yl)methyl)-5-(4-((N-methylacetamido)methyl)benzyl)nicotinamide as a white solid (107.7 mg, 81%). LRMS (M+H+) m/z calculated 462.17, found 462.4. ¾ NMR (DMSO-d6, 300 MHz) δ 13.08 (s, 1 H), 9.30 (t, 1 H), 8.87 (s, 1 H), 8.63 (s, 1 H), 8.06 (s, 1 H), 7.90 (s, 1 H), 7.53 (d, 1 H), 7.33 (dd, 1 H), 7.28-7.21 (m, 2 H), 7.15-7.11 (m, 2 H), 4.78 (d, 2 H), 4.49 (s, 1 H), 4.42 (s, 1 H), 4.00 (d, 2 H), 2.86 (s, 2 H), 2.75 (s, 1 H), 2.08-2.01 (m, 3 H).
Example 128: Preparation of N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-((N- methylacetamido)methyl)benzyl)nicotinamide
Figure imgf000228_0002
A mixture of 5-(4-((N-methylacetamido)methyl)benzyl)nicotinic acid (277 mg, crude, 0.48 mmol, 1.0 eq), (3- chloro-lH-indol-5-yl)methanamine HCl (104 mg, 0.48 mmol, 1.0 eq), HATU (274 mg, 0.72 mmol,1.5 eq) and DIEA (185 mg, 1.44 mmol, 3.0 eq) in DMF (5 mL) was stirred at rt for 2 h. The mixture was purified directly by flash chromatography to afford N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-((N- methylacetamido)methyl)benzyl)nicotinamide as a white solid (86.6 mg, 39%>). LRMS (M+H+) m/z calculated 461.17, found 461.2. ¾ NMR (DMSO-d6, 400 MHz) δ 13.33 (s, 1 H), 9.21 (t, 1 H), 8.89 (s, 1 H), 8.64 (s, 1 H), 8.07 (s, 1 H), 7.50 (s, 1 H), 7.42 (s, 1 H), 7.37 (d, 1 H), 7.27 (d, 1 H), 7.22 (d, 1 H), 7.18-7.12 (m, 3 H), 4.56 (d, 2 H), 4.50 (s, 0.8H), 4.43 (s, 1.3H), 4.01 (d, 2 H), 2.86 (s, 2 H), 2.75 (s, 1 H), 2.08-2.01 (m, 3 H).
Example 129: Preparation of (S)-5-(4-(l -acetamidoethyl)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000229_0001
(S)"5"(4"(1"acetamidoethyl)benzy|J"w"((3"ch "1 HΊndoΓ5 l)methy|)nicotinamic|e
Figure imgf000229_0002
To the solution of (S)-l-(4-bromophenyl)ethanamine (2.0 g, 10.0 mmol, 1.0 eq) and pyridine (1.2 mL, 15.0 mmol, 1.5 eq) in DCM (5 mL) was added acetic anhydride (1.4 mL, 15.0 mmol, 1.5 eq) at 0 °C. The reaction was stirred at rt for 2 h, quenched with water, and the DCM layer was separated, washed twice with 2 N HC1, water, brine, dried over anhydrous sodium sulfate, and purified on silica gel column (PE/EA = 2/1) to afford (S)-N-(l -(4-bromo henyl)ethyl)acetamide as a white solid 1.8 g, 75%).
Figure imgf000229_0003
The mixture of (S)-N-(l-(4-bromophenyl)ethyl)acetamide (710 mg, 2.920 mmol, 1.3 eq), 4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl-4',4',5',5'-tetramethyl-l,3,2-dioxaborolane (743 mg, 2.92 mmol, 1.3 eq), potassium acetate (860 mg, 8.76 mmol, 3.0 eq) and (l, -bis(diphenylphosphino)ferrocene)palladium(II) dichloride (105 mg, 5 mol%) in 1,4-dioxane (20 mL) was degassed and stirred at 80 °C for 6 h, cooled to rt, methyl 5- (chloromethyl)nicotinate hydrochloride (500 mg, 2.25 mmol, 1.0 eq), (Ι,Γ- bis(diphenylphosphino)ferrocene)palladium(II) dichloride (105 mg, 5 mol%) and an aqueous sodium carbonate (1.50 g, 15.0 mmol, 5.0 eq) in water (4 mL) were added successively. The mixture was degassed again and stirred at 65 °C overnight, cooled, filtered through celite, rinsed with DCM. The DCM layer was washed with water, brine, dried over anhydrous sodium sulfate, and purified on silica gel column (PE/EA = 2/1) to afford (S)-methyl 80 mg, 63%).
Figure imgf000229_0004
The solution of (S)-methyl 5-(4-(l-acetamidoethyl)benzyl)nicotinate (580 mg, 1.85 mmol, 1.0 eq) in THF (10 mL) was added an aqueous solution of sodium hydroxide (148 mg, 3.7 mmol, 2.0 eq) in water (4 mL). The reaction was stirred for 1 h. The mixture was acidified with 2 N HC1 to pH 2, concentrated. The residue was washed with 10% MeOH in DCM, filtered, and the filtrate was dried over anhydrous sodium sulfate and concentrated to dryness to afford (S)-5-(4-(l -acetamidoethyl)benzyl)nicotinic acid as a yellow solid (550 mg, quantitative).
Figure imgf000230_0001
To the solution of (S)-methyl 5-(4-(l-acetamidoethyl)benzyl)nicotinate (140 mg, 0.47 mmol, 1.0 eq) in DMF (4 mL) was added HATU (232 mg, 0.61 mmol, 1.3 eq), DIEA (0.7 mL) and (3-chloro-lH-indol-5- yl)methanamine hydrochloride (110 mg, 0.5 mmol, 1.0 eq) successively. The reaction was purified via flash chromatography to afford (S)-5-(4-(l-acetamidoethyl)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide as a yellow solid (90 mg, 41%). LRMS (M+H)+ m/z calculated 461.1, found 461.1.1H NMR (DMSO-ifc, 400 MHz) δ 11.31 (s, 1 H), 9.19 (t, 1 H), 8.88 (s, 1 H), 8.63 (s, 1 H), 8.20 (d, 1 H), 8.06 (s, 1 H), 7.49 (s, 1 H), 7.42 (s, 1 H), 7.37 (d, 1 H), 7.23-7.19 (m, 4 H), 7.17 (d, 1 H), 4.86-4.81 (m, 1 H), 4.56 (d, 1 H), 3.99 (s, 1 H), 1.79 (s, 3 H), 1.29 (d, 3 H). Example 130: Preparation of (S)-5-(4-(l -acetamidoethyl)benzyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide
Figure imgf000230_0002
To the solution of (S)-methyl 5-(4-(l-acetamidoethyl)benzyl)nicotinate (298 mg, 1.0 mmol, 1.0 eq) in DMF (4 mL) was added HATU (495 mg, 1.3 mmol, 1.3 eq), DIEA (1.0 mL) and (5-chloro-l -(tetrahydro-2H-pyran-2- yl)-lH-indazol-3-yl)methanamine (265 mg, 1.0 mmol, 1.0 eq) successively. The reaction was stirred for 1 h, added to ice-water dropwise with stirring, and the precipitate was collected by filtration, dried to afford 5-(4- ((S)-l-acetamidoethyl)benzyl)-N-((5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)nicotinamide as a yellow solid (400 mg, 73%>).
Figure imgf000231_0001
5-(4-((S)-l -acetamidoethyl)benzyl)-N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methyl)nicotinamide (400 mg, 0.73 mmol, 1.0 eq) was treated with trifluoroacetic aicd (2.5 mL) and DCM (2.5 mL) with stirring for 1 h and then it was concentrated. The residue was washed with saturated aqueous sodium bicarbonate carefully, and the solid was collected and purified via flash chromatography to afford (S)- 5-(4-(l -acetamidoethyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)nicotinamide as a yellow solid (150 mg, 41%). LRMS (M+H)+ m/z calculated 461.1, found 461.1.1H NMR (DMSO-d6, 400 MHz) δ 11.31 (s, 1 H), 9.19 (t, 1 H), 8.88 (s, 1 H), 8.63 (s, 1 H), 8.20 (d, 1 H), 8.06 (s, 1 H), 7.49 (s, 1 H), 7.42 (s, 1 H), 7.37 (d, 1 H), 7.23-7.19 (m, 4 H), 7.17 (d, 1 H), 4.86-4.81 (m, 1 H), 4.56 (d, 1 H), 3.99 (s, 1 H), 1.79 (s, 3 H), 1.29 (d, 3 H).
Example 131: Preparation of 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)-N-((6-fluoro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000231_0002
5 (4 (1 acetyipyrroiidin 2 yhbenzyh N ^ fiuoro I H
indof5" l)methyi)ni∞tir(amic|e
Figure imgf000231_0003
To the solution of 2-(4-bromophenyl)pyrrolidine (914 mg, 4.04 mmol, 1.0 eq) and TEA (2.5 mL, 18 mmol, 4 eq) in DCM (15 mL) was added AcCl (0.38 mL, 5.35 mmol, 1.3 eq). The mixture was stirred at rt overnight and washed with water (20 mL), dried over anhydrous Na2S04 and concentrated to afford l-(2-(4- bromophenyl)pyrrolidin-l -yl)ethanone as a yellow oil (1.14 g crude, quant).
Figure imgf000231_0004
A mixture of l-(2-(4-bromophenyl)pyrrolidin-l-yl)ethanone (1.14 g, 4 mmol, 1.0 eq), 4,4,4',4',5,5,5',5'- octamethyl-2,2'-bi(l,3,2-dioxaborolane) (1.07 g, 4.2 mmol, 1.05 eq), Pd(dppf)Cl2 CH2C12 (327 mg, 0.4 mmol, 0.1 eq), KOAc (1.18 g, 12 mmol, 3 eq) in 1,4-dioxane (20 mL) under N2 was stirred at 85 °C overnight. The mixture was and concentrated and the residue was purified on silica gel column (EA/PE = 2/3, v/v) to afford l-(2-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)pyrrolidin-l -yl)ethanone as a yellow oil (1.39 g crude, quant).
Figure imgf000232_0001
The reaction mixture of l -(2-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)pyrrolidin-l -yl)ethanone (1.39 g, 4 mmol, leq), ethyl 5-(chloromethyl)nicotinate hydrochloride (932 mg, 4.2 mmol, 1.05 eq),
Pd(dppf)Cl2 CH2Cl2 (327 mg, 0.4 mmol, 0.1 eq) and Na2C03 (2.12 g, 20 mmol, 5 eq) in 1 ,4-dioxane/H20 (15 mL/1.5 mL) was degassed with N2 and stirred at 80 °C overnight .The mixture was filtered and concentrated. The residue was purified on silica gel column (EA/PE = 1/1-1/0, v/v) to afford ethyl 5-(4-(l -acetylpyrrolidin- 2-yl)benzyl)nicotinate as a yellow oil (973 mg, 72%).
Figure imgf000232_0002
To a solution of ethyl 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)nicotinate (973 mg, 2.88 mmol, 1.0 eq) in MeOH (5 mL) and THF (10 mL) was added a solution of LiOH (400 mg, 9.52 mmol, 3.3 eq) in water (5 mL). The mixture was stirred at rt for 3 h. The organic solvents were evaporated and the residue was acidified by HCl to pH 3. The mixture was concentrated to afford 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)nicotinic acid as a yellow solid (1.9 g, mixe
Figure imgf000232_0003
A mixture of 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)nicotinic acid (162 mg, 0.5 mmol, 1.0 eq), (6-fluoro-lH- indol-5-yl)methanamine (82 mg, 0.5 mmol, 1.0 eq), HATU (228 mg, 0.6 mmol, 1.2 eq) and DIPEA (0.8 mL) in DMF (3 mL) was stirred at rt overnight. The mixture was purified via prep-MPLC to afford 5-(4-(l - acetylpyrrolidin-2-yl)benzyl)-N-((6-fluoro-lH-indol-5-yl)methyl)nicotinamide as a white solid (136 mg, 57.8%). LRMS (M+H+) m/z calculated 471.2, found 471.2. ¾ NMR (DMSO-d6, 400 MHz) δ 11.10 (s, 1 H), 9.12 (t, 1 H), 8.90 (dd, 1 H), 8.64 (dd, 1 H), 8.10 (d, 1 H), 7.50 (d, 1 H), 7.31 (t, 1 H), 7.25 (d, 1 H), 7.18 (d, 1 H), 7.15 (d, 1 H), 7.11 (d, 1 H), 7.06 (d, 1 H), 6.40 (s, 1 H), 4.97 (t, 1 H) , 4.55 (d, 2 H), 4.00 (d, 2 H), 3.73- 3.45 (m, 2 H), 2.33-2.13 (m, 1 H), 2.00 (s, 1.5 H), 1.86-1.67 (m, 3 H), 1.66 (s, 1.5 H).
Example 132: Preparation of 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000233_0001
To the solution of 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)-N-((6-fluoro-lH-indol-5-yl)methyl)nicotinamide (95 mg, 0.2 mmol, 1.0 eq) in DCM (5 mL) was added NCS (56 mg, 0.42 mmol, 2.1 eq). The mixture was stirred at rt for 2 h and concentrated. The residue was purified via prep-MPLC to afford5-(4-(l -acetylpyrrolidin-2- yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)nicotinamide as a white solid (40 mg, 39.2%). LRMS (M+H+) m z calculated 505.2, found 505.2. ¾ NMR (DMSO-d6, 400 MHz) δ 11.39 (s, 1 H), 9.16 (t, 1 H), 8.89 (dd, 1 H), 8.64 (dd, 1 H), 8.09 (d, 1 H), 7.50 (d, 1 H), 7.45 (d, 1 H), 7.25 (d, 1 H), 7.22 (d, 1 H), 7.17 (d, 1 H), 7.11 (d, 1 H), 7.06 (d, 1 H), 4.97 (t, 1 H) , 4.59 (d, 2 H), 4.00 (d, 2 H), 3.73-3.45 (m, 2 H), 2.33-2.13 (m, 1 H), 2.00 (s, 1.5 H), 1.87-1.70 (m, 3 H), 1.65 (s, 1.5 H).
Example 133: Preparation of 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000233_0002
5"(4 "(racety|pyrro|idin yKbenzyK"/ " ((3"ch|oro"i H"indor5'
yhmethyhnicotitiamide
Figure imgf000234_0001
A mixture of 5-(4-(l -acetylpyrrolidin-2-yl)benzyl)nicotinic acid (162 mg, 0.5 mmol, 1.0 eq), (3-chloro-lH- indol-5-yl)methanamine hydrochloride (117 mg, 0.5 mmol, 1.0 eq), HATU (228 mg, 0.6 mmol, 1.2 eq) and DIPEA (1 mL) in DMF (3 mL) was stirred at rt overnight. The mixture was purified via prep-MPLC to afford 5-(4-(l -acetylpyrrolidin-2-yl)benzyl)-N-((3-chloro-lH-indol-5-yl)methyl)nicotinamide as a white solid (120 mg, 49.4%). LRMS (M+H+) m/z calculated 487.2, found 487.5. ¾ NMR (DMSO-d6, 400 MHz) δ 1 1.32 (s, 1 H), 9.20 (t, 1 H), 8.89 (dd, 1 H), 8.63 (dd, 1 H), 8.09-8.08 (m, 1 H), 7.49 (d, 1 H), 7.43 (s, 1 H), 7.37 (d, 1 H), 7.25 (d, 1 H), 7.17 (d, 2 H), 7.11 (d, 1 H), 7.06 (d, 1 H), 4.97 (t, 1 H) , 4.56 (d, 2 H), 4.00 (d, 2 H), 3.73-3.45 (m, 2 H), 2.37-2.13 (m, 1 H), 2.00 (s, 1.5 H), 1.87-1.66 (m, 3 H), 1.65 (s, 1.5 H).
Example 134: Preparation of 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide
Figure imgf000234_0002
5 (4 (1 acetyipyi-roiidin 2 yhbenzyk N 5 chioro I H indazoi 3 yhmethyhnicotinamide
h drochloride
Figure imgf000234_0003
A mixture of 5-(4-(l -acetylpyrrolidin-2-yl)benzyl)nicotinic acid (162 mg, 0.5 mmol, 1.0 eq), (5-chloro-l- (tetrahydro-2H-pyran-2-yl)-lH-indazol-3-yl)methanamine (133 mg, 0.5 mmol, 1.0 eq), HATU (228 mg, 0.6 mmol, 1.2 eq) and DIPEA (0.8 mL) in DMF (3 mL) was stirred at rt overnight. The mixture was purified via prep-MPLC to afford 5-(4-( 1 -acetylpyrrolidin-2-yl)benzyl)-N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H- indazol-3-yl)methyl)
Figure imgf000234_0004
A solution of 5-(4-( 1 -acetylpyrrolidin-2-yl)benzyl)-N-((5-chloro- 1 -(tetrahydro-2H-pyran-2-yl)- 1 H-indazol-3- yl)methyl)nicotinamide (100 mg, 0.175 mmol, 1 eq) in MeOH (0.5 mL) and HC1/EA (5 N, 2 mL) was stirred at rt overnight, then filtered. The solid was collected to afford 5-(4-(l-acetylpyrrolidin-2-yl)benzyl)-N-((5- chloro-lH-indazol-3-yl)methyl)nicotinamide hydrochloride as a yellow solid (47 mg, 51.2%).LRMS (M+H+) m/z calculated 488.2, found 488.1. ¾ NMR (CD3OD, 400 MHz) δ 9.18 (d, 1 H), 8.94 (s, 1 H), 8.90 (d, 1 H), 7.93 (s, 1 H), 7.53 (d, 1 H), 7.40 (dd, 1 H), 7.37 (d, 1 H), 7.29 (d, 1 H), 7.23 (d, 1 H), 7.19 (d, 1 H), 5.16 - 5.12 (m, 1 H), 4.98 (s, 2 H) , 4.30 (d, 2 H), 3.89-3.68 (m, 2 H), 2.50-2.33 (m, 1 H), 2.21 (s, 1.5 H), 2.04-1.85 (m, 3 H), 1.86 (s, 1.5 H). Example 135: Preparation of (R)-5-(4-(l -acetamidoethyl)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000235_0001
(R) 5 (4 (1 acetam oethyl)benzy| N ^3 chioro H \^ύΡ\ 5 yljmethyljnicotinamide
Figure imgf000235_0002
To a solution of (R)-l-(4-bromophenyl)ethanamine (2.0 g, 10 mmol, 1.0 eq) in DCM (20 mL) were added pyridine (1.19 g, 15 mmol, 1.5 eq) and AC2O (1.53 g, 15 mmol, 1.5 eq) at 0 °C under N2. The suspension was stirred at rt overnight. The reaction mixture was added 1 N HCl (20 mL), extracted with EA (20 mL x3). The combined organic layers were washed with brine (10 mL), dried over anhydrous Na2S04 and concentrated. The residue was purified via flash chromatography to afford (R)-N-(l-(4-bromophenyl)ethyl)acetamide as a white solid (2.0 g, 83%).
Figure imgf000235_0003
To a solution of (R)-N-(l -(4-bromophenyl)ethyl)acetamide (653 mg, 2.69 mmol, 1.0 eq) in 1,4-dioxane (30 mL) were added KOAc (794 mg, 8.1 mmol, 3.0 eq), 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2-dioxaborolane) (686 mg, 2.69 mmol, 1.0 eq) and Pd(dppf)Cl2 (99 mg, 0.14 mmol, 0.05 eq). The mixture was heated at 85 °C overnight under N2, and then the mixture was cooled to rt. To the mixture were added methyl 5- (chloromethyl)nicotinate (500 mg, 2.69 mmol, 1.0 eq), Pd(dppf)Cl2 (99 mg, 0.14 mmol, 0.05 eq) and Na2C03 (859 mg, 8.1 mmol, 3.0 eq) in water (6 mL). The mixture was heated to 80 °C overnight under N2. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (10 mL) and extracted with EA (10 mL x 3). The combined organic layers were washed with brine (5 mL), dried over anhydrous Na2SC>4 and concentrated. The residue was purified on silica gel column (DCM/MeOH = 200/1 to 50/1) to afford (R)-methyl 5-(4-(l -acetamidoethyl)benzyl)nicotinate as a yellow solid (320 mg, 38%).
Figure imgf000236_0001
To a solution of (R)-methyl 5-(4-(l -acetamidoethyl)benzyl)nicotinate (320 mg, 1.03 mmol, 1.0 eq) in MeOH (4 mL) and H2O (4 mL) was added NaOH (124 mg, 3.09 mmol, 3.0 eq). The mixture was heated at 60 °C for 30 min. The mixture was concentrated to remove MeOH, then diluted with ice-water (5 mL) and acidified with 1 N HCl to pH 2-3. The mixture was concentrated to dryness under reduced pressure. The residue was re- dissolved in MeOH (10 mL) and filtered. The filtrate was concentrated under reduced pressure to afford (R)- 5-(4-(l -acetamidoethyl
Figure imgf000236_0002
To a solution of (R)-5-(4-(l-acetamidoethyl)benzyl)nicotinic acid (250 mg crude, 0.52 mmol, 1.0 eq) in DMF (3 mL) were added DIPEA (335 mg, 2.6 mmol, 5.0 eq) and HATU (237 mg, 0.62 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (3-chloro-lH-indol-5-yl)methanamine (113 mg, 0.52 mmol, 1.0 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was purified via flash chromatography to afford (R)-5-(4-(l -acetamidoethyl)benzyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide as a white solid (122 mg, 51%). LRMS (M+H+) m/z caculated 461.1, found 461.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.32 (brs, 1 H), 9.20 (t, 1 H), 8.88 (d, 1 H), 8.63 (d, 1 H), 8.20 (d, 1 H), 8.06 (s, 1 H), 7.49 (d, 1 H), 7.42 (s, 1 H), 7.37 (d, 1 H), 7.30-7.20 (m, 4 H), 7.15 (d, 1 H), 4.90-4.80 (m, 1 H), 4.56 (d, 2 H), 3.99 (s, 2 H), 1.79 (s, 3 H), 1.28 (d, 3 H).
Example 136: Preparation of (R)-5-(4-(l -acetamidoethyl)benzyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide
Figure imgf000236_0003
(R)"5"(4 "(1"acΘtamidOΘthyl)benzy|j "/ "((5"ch|oro"1HΊndazo|Yy|)mΘthyl)nicotinamidΘ
Figure imgf000237_0001
To a solution of (R)-5-(4-(l-acetamidoethyl)benzyl)nicotinic acid (250 mg crude, 0.52 mmol, 1.0 eq) in DMF (3 mL) were added DIPEA (335 mg, 2.6 mmol, 5.0 eq) and HATU (237 mg, 0.62 mmol, 1.2 eq) at 0 °C under N2. The mixture was stirred at 0 °C for 30 min, (5-chloro-l -(tetrahydro-2H-pyran-2-yl)-lH-indazol-3- yl)methanamine (138 mg, 0.52 mmol, 1.0 eq) was then added and the mixture was stirred at rt overnight under N2. The mixture was purified via flash chromatography to afford 5-(4-((R)-l-acetamidoethyl)benzyl)-N-((5- chloro-l-(tetrahydro- lid (220 mg, 77%).
Figure imgf000237_0002
To a solution of 5-(4-((R)-l-acetamidoethyl)benzyl)-N-((5-chloro-l-(tetrahydro-2H-pyran-2-yl)-lH-indazol- 3-yl)methyl)nicotinamide (220 mg, 0.4 mmol, 1.0 eq) in MeOH (2 mL) was added HCl/MeOH (2 mL) at 0 °C. The mixture was stirred at rt for 2 h. The mixture was concentrated to dryness under reduced pressure. The residue was mixed with water (5 mL), neutralized with saturated NaHC03 (5 mL) and concentrated. The residue was purified via flash chromatography to afford (R)-5-(4-(l-acetamidoethyl)benzyl)-N-((5-chloro-lH- indazol-3-yl)methyl)nicotinamide as a white solid (102 mg, 55%). LRMS (M+H+) m/z caculated 462.1, found 462.1. 'H NMR (DMSO-ifc, 300 MHz) δ 13.08 (brs, 1 H), 9.30 (t, 1 H), 8.86 (d, 1 H), 8.63 (d, 1 H), 8.21 (d, 1 H), 8.06 (s, 1 H), 7.90 (d, 1 H), 7.53 (d, 1 H), 7.34 (dd, 1 H), 7.30-7.20 (m, 4 H), 4.90-4.80 (m, 1 H), 4.78 (d, 2 H), 3.98 (s, 2 H), 1.80 (s, 3 H), 1.28 (d, 3 H).
Example 137: Preparation of 2-(5-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridine-3-yl) methyl)- lH-indol-3-yl)acetic acid
Figure imgf000237_0003
To a solution of (5-bromo-lH-indol-3-yl)-acetic acid (2.8 g, 11.0 mmol, 1 eq) in i-BuOH (20 mL) /THF (60 mL) was added B0C2O (12.0 g, 55.0 mmol, 5 eq) and DMAP (134 mg, 1.1 mmol, 0.1 eq). The mixture was stirred at 80 °C overnight. The reaction mixture was concentrated in vacuo. And the residue was purified by chromatography on a silica gel column (EA/PE = 1/ 100) to give 5-bromo-3-tert-butoxycarbonylmethyl- indole-l -carboxylic acid tert-butyl ester (1.29 g, 29%) as a yellow solid.
Figure imgf000238_0001
To a solution of 5-bromo-3-tert-butoxycarbonylmethyl-indole-l -carboxylic acid tert-butyl ester (1.20 g, 2.93 mmol, 1 eq) and B2(Pin)2 (895 mg, 3.52 mmol, 1.2 eq) in dioxane (25 mL) was added KOAc (862 mg, 8.79 mmol, 3 eq) and Pd(dppf)Ci2.CH2Ci2 (120 mg, 0.15 mmol, 0.05 eq). The mixture was stirred at 95 °C for 2 h and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column to give compound 3-tert-butoxycarbonylmethyl-5-(4,4,5,5-tetramethyl-[l,3,2]dioxaborolan- 2-yl)-indole-l-carbo
Figure imgf000238_0002
To a solution of 3-tert-butoxycarbonylmethyl-5-(4,4,5,5-tetramethyl-[l,3,2]dioxaborolan-2-yl)-indole-l- carboxylic acid tert-butyl ester (1.14 g, 2.5 mmol, 1 eq) and 5-chloromethyl-nicotinic acid methyl ester (555 mg, 2.5 mmol, 1 eq) in dioxane (25 mL) and water (5 mL) was added K2CO3 (1.38 g, 10.0 mmol, 4 eq), and Pd(PPli3)4 (150 mg, 0.125 mmol, 0.05 eq). The mixture was stirred at 95 °C for 1 h under nitrogen and then it was diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (EA/ PE = 1/5) to give 3-tert-butoxycarbonylmethyl-5-(5-methoxycarbonyl-pyridin-3- ylmethyl)-indole-l-carboxylic acid tert-butyl ester (1.02 g, 85%) as a colorless oil.
Figure imgf000238_0003
To a solution of 3-tert-butoxycarbonylmethyl-5-(5-methoxycarbonyl-pyridin-3-ylmethyl)-indole-l-carboxylic acid tert-butyl ester (1.0 g, 2.08 mmol, 1 eq) in THF (7 mL), MeOH (7 mL) and H20 (7 mL) was added
L1OH.H2O (96 mg, 2.29 mmol, 1.1 eq). The mixture was stirred at rt for 2 h and was acidified to pH 5-6 with 1 N HC1 solution. The mixture was extracted with EA and the combined organic layer was dried and concentrated. The residue (945 mg, quant) as a white solid was directly used without further purification.
Figure imgf000239_0001
To a solution of 3-tert-butoxycarbonylmethyl-5-(5-carboxy-pyridin-3-ylmethyl)-indole-l-carboxylic acid tert- butyl ester (200 mg, 0.43 mmol, 1 eq) and c-(3-chloro-lH-indol-5-yl)-methylamine (102 mg, 0.473 mmol, 1.1 eq) in DCM (5 mL) was added HOBT (88 mg, 0.645 mmol, 1.5 eq), EDCI (124 mg, 0.645 mmol, 1.5 eq) and Et3N (0.18 mL, 1.29 mmol, 3 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (EA/PE = 1/ 1) to give 3 -tert-butoxycarbonylmethyl-5 - { 5- [(3 -chloro- 1 H-indol-5 -ylmethyl)-carbamoyl] -pyridin-3 -ylmethyl} -indole- -carboxylic acid tert-butyl ester (200 mg, 74%) as a white solid.
Figure imgf000239_0002
3-tert-Butoxycarbonylmethyl-5- {5-[(3-chloro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-3-ylmethyl} -indole- 1 -carboxylic acid tert-butyl ester (80 mg, 0.127 mmol) was treated with HCOOH (4 mL) and the mixture was stirred at rt for 30 h. The mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 2-(5-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-yl)methyl)-lH-indol-3-yl)acetic acid (19 mg, 32%) as a white solid. LRMS (M+H+) m/z calculated 473.1, found 473.1. ¾ NMR (CD3OD, 400 MHz) δ 8.77 (s, 1 H), 8.51 (s, 1 H), 8.10 (s, 1 H), 7.47 (s, 1 H), 7.40 (s, 1 H), 7.30 (d, 1 H), 7.25 (d, 1 H), 7.20 (s, 1 H), 7.15 (dd, 1 H), 7.13 (s, 1 H), 6.91 (dd, 1 H), 4.60 (s, 2 H), 4.07 (s, 2 H), 3.68 (s, 2 H).
Example 138: Preparation of 2-(5-((5-(((5-chloro-lH-indazol-3-yl)methyl)carbamoyl) pyridine-3-yl)methyl)- 1 H-indol-3-yl)acetic acid
Figure imgf000239_0003
To a solution of 3-tert-butoxycarbonylmethyl-5-(5-carboxy-pyridin-3-ylmethyl)-indole-l -carboxylic acid tert- butyl ester (230 mg, 0.49 mmol, 1 eq) and C-(5-chloro-lH-indazol-3-yl)-methylamine (108 mg, 0.59 mmol, 1.2 eq) in DCM (5 mL) was added HOBT (100 mg, 0.74 mmol, 1.5 eq), EDCI (142 mg, 0.74 mmol, 1.5 eq) and Et3N (0.21 mL, 1.48 mmol, 3 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (EA/PE 1/1) to give 3-tert-butoxycarbonylmethyl-5- {5-[(5-chloro-lH-indazol-3-ylmethyl)-carbamoyl]-pyridin-3- ylmethyl}-indole-
Figure imgf000240_0001
5 3-tert-Butoxycarbonylmethyl-5- {5-[(5-chloro-lH-indazol-3-ylmethyl)-carbamoyl]-pyridin-3-ylmethyl}- indole- 1 -carboxylic acid tert-butyl ester (80 mg, 0.128 mmol) was treated with HCOOH (4 mL) and the mixture was stirred at rt for 30 h. The mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 2-(5-((5-(((5-chloro-lH-indazol-3-yl)methyl)carbamoyl)pyridin-3-yl)methyl)-l H-indol-3- yl)acetic acid (25 mg, 42%) as an off-white solid. LRMS (M+H+) m/z calculated 474.1, found 474.1. 1 H 10 NMR (CD3OD, 400 MHz) δ 8.82 (s, 1 H), 8.58 (s, 1 H), 8.13 (s, 1 H), 7.88 (d, 1 H), 7.49 (d, 1 H), 7.46 (s, 1 H), 7.35 (dd, 1 H), 7.31 (d, 1 H), 7.18 (s, 1 H), 6.98 (dd, 1 H), 4.90 (s, 2 H), 4.16 (s, 2 H), 3.73 (s, 2 H).
Example 139: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l -(2,3-difluoro-4 -((2-oxopyrazin- l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydr e
Figure imgf000240_0002
Figure imgf000240_0003
To a solution of (2,3-difluoro-phenyl)-methanol (50 g, 0.35 mol, 1.0 eq) and imidazole (70.9 g, 1.05 mol, 3.0 eq) in dry THF (1 L) was added TBDMSCI (62.7 g, 0.42 mol, 1.2 eq) at ice-bath. After stirring at rt for 3 h, the mixture was extracted with EA, and washed with water and 0.5 N HC1. The organic layer was dried over Na2S04 and evaporated to give the crude product was directly used in next step.
Figure imgf000240_0004
A 1.3 N solution of ί-BuLi in THF (292 mL, 0.38 mol, 1.1 eq) was added to a solution of tert-butyl-(2,3- difluoro-benzyloxy)-dimethyl-silane (90 g, 0.35 mol, 1.0 eq) in dry THF (800 mL) at -78 °C under argon atmosphere. After stirring for 2 h, DMF (28 g, 0.38 mol, 1.1 eq) was added. The mixture was stirred at room 5 temperature overnight and was quenched with saturated aqueous NaCl and extracted with EA. The organic layer was dried over MgS04 and concentrated to give 4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3-difluoro- benzaldehyde (93 g) as a colorless oil.
Figure imgf000241_0001
To a solution of 4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3-difluoro-benzaldehyde (93 g, 0.32 mol, 1.0 eq) in THF (800 mL) was added NaBH4 (8.6 g, 0.22 mol, 0.7 eq) at ice-bath,. And then methanol was added dropwise until LRMS showed the SM was consumed. The reaction mixture was quenched with saturated aqueous NaCl and extracted with ethyl acetate. The organic layer was dried over MgS04 and concentrated give the crude product which was subjected to silica gel chromatography (PE/ EA = 20/ 1 -10/ 1) to afford [4- (tert-butyl-dimethyl-silanyloxymethyl -2,3-difluoro-phenyl]-methanol (42 g, 45%) as a yellow oil.
Figure imgf000241_0002
To a solution of [4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3-difluoro-phenyl] -methanol (10 g, 0.03 mol, 1.0 eq) in DCM (200 mL) was added PPh3 (10.4 g, 0.04 mol, 1.15 eq) and NBS (7.1 g, 0.04 mol, 1.15 eq). The mixture was stirred at room temperature overnight. The reaction mixture was quenched with saturated aqueous NaCl and extracted with CH2CI2. The organic layer was dried over MgSC and concentrated to give the crude product which was subject to silica gel chromatography (DCM/ MeOH = 300/ 1 -200/1) to afford (4-bromomethyl-2,3-difluoro-ph d.
Figure imgf000241_0003
To a solution of (4-bromomethyl-2,3-difluoro-phenyl)-methanol (4.4 g, 0.02 mol, 1.0 eq) in DMF (100 mL) was added 6-hydroxy-nicotinic acid methyl ester (2.84 g, 0.02 mmol, 1.0 eq) and K2CO3 (8.28 g, 0.06 mmol, 3.0 eq). The reaction mixture was stirred overnight at room temperature, and was quenched with saturated aqueous NaCl and extracted with ethyl acetate. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (DCM/ MeOH = 200/ 1) to afford 1- (2,3-difluoro-4-hydroxymethyl-benzyl)-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (4 g, 70%>) as a yellow solid.
Figure imgf000241_0004
To a solution of l -(2,3-difluoro-4-hydroxymethyl-benzyl)-6-oxo-l ,6-dihydro-pyridine-3-carboxylic acid methyl ester (2 g, 6.47 mmol, 1.0 eq) in DCM (30 mL) was added PPh3 (1.95 g, 7.44 mmol, 1.15 eq) and CBr4 (2.47 g, 7.44 mmol, 1.15 eq). The mixture was stirred at room temperature overnight. The reaction mixture was quenched with saturated aqueous NaCl and extracted with CH2CI2. The organic layer was dried over MgS04 and concentrated to give the crude product which was subject to silica gel chromatography (DCM/ MeOH = 300/ 1 -200/ 1) to afford l -(4-bromomethyl-2,3-difluoro-benzyl)-6-oxo-l ,6-dihydro-pyridine-3- carboxylic acid methyl ester (2.1 g, 87%) as a yellow solid.
Figure imgf000242_0001
To a solution of l -(4-bromomethyl-2,3-difluoro-benzyl)-6-oxo-l ,6-dihydro-pyridine-3-carboxylic acid methyl ester (2.1 g, 5.66 mmol, 1.0 eq) in DMF (30 mL) was added pyrazin-2-ol (543 mg, 5.66 mmol, 1.0 eq) and K2CO3 (2.34 g, 17 mmol, 3.0 eq). The reaction mixture was stirred overnight at room temperature, and was quenched with saturated aqueous NaCl and extracted with ethyl acetate. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography
(DCM/MeOH = 20/1) to afford l -[2,3-difluoro-4-(2-oxo-2H-pyrazin-l -ylmethyl)-benzyl]-6-oxo-l ,6-dihydro- pyridine-3-carboxylic acid methy solid.
Figure imgf000242_0002
To a solution of l -[2,3-difluoro-4-(2-oxo-2H-pyrazin-l -ylmethyl)-benzyl]-6-oxo-l ,6- dihydro-pyridine-3- carboxylic acid methyl ester (840 mg, 2.16 mmol, 1.0 eq) in THF (18 mL)/ H20 (2 mL) was added LiOH.H20 (100 mg, 2.38 mmol, 1.1 eq). The mixture was stirred at 40 °C for 30 min and was acidified to pH 5-6 with 1 N HC1 solution. The mixture was concentrated in vacuo and the residue (800 mg, 99%) as a white solid was directly used without further purification.
Figure imgf000242_0003
To a solution of l -[2,3-difluoro-4-(2-oxo-2H-pyrazin-l -ylmethyl)-benzyl]-6-oxo-l ,6-dihydro-pyridine-3- carboxylic acid (80 mg, 0.214 mmol, 1.0 eq) and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (50 m 0.214 mmol, 1.0 eq) in DCM (4 mL) was added HATU (98 mg, 0.257 mmol, 1.2 eq) and Et3N (0.12 mL, 0.856 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and
concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 10/ 1) to give N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-l -(2,3-difluoro-4-((2-oxopyrazin-l(2H)- yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide (62 mg, 52%) as a white solid. LRMS (M+H+) m/z calculated 554.1, found 554.1. Ή NMR ( CD =00, 400 MHz) δ 8.42 (d, 1 H), 8.07 (d, 1 H), 8.00 (dd, 1 H), 7.62 (d, 1 H), 7.52 (d, 1 H), 7.42 (d, 1 H), 7.27 (s, 1 H), 7.14 (d, 1 H), 7.15-7.06 (m, 2 H), 6.57 (d, 1 H), 5.30 (s, 2 H), 5.22 (s, 2 H), 4.69 (s, 2 H).
Example 140: Preparation of l-(2,3-difluoro-4-((2-oxopyrazin-l(2H)-yl)methyl)benzyl)-N-((6-fluoro-l H- indol-5 -yl)methyl) -6-ox -l , 6-dihydropyridine-3 -carboxamide
Figure imgf000243_0001
To a solution of l-[2,3-difluoro-4-(2-oxo-2H-pyrazin-l-ylmethyl)-benzyl]-6-oxo-l,6-dihydro -pyridine-3- carboxylic acid (80 mg, 0.214 mmol, 1.0 eq) and C-(6-fluoro-l H-indol-5-yl)-methylamine (50 mg, 0.214 mmol, 1.0 eq) in DCM (4 mL) was added HATU (98 mg, 0.257 mmol, 1.2 eq) and Et3N (0.12 mL, 0.856 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/MeOH = 10/1) to give l-(2,3-difluoro-4-((2- oxopyrazin-l(2 H)-yl)methyl)benzyl)-N-((6-fluoro-l H-indol-5-yl)methyl)-6-oxo-l,6-dihydropyridine-3- carboxamide (25 mg, 23%) as a white solid. LRMS (M+H+) m/z calculated 520.1.1, found 520.1. ¾ NMR (CD3OD, 400 MHz) δ 8.40 (d, 1 H), 8.07 (d, 1 H), 8.00 (dd, 1 H), 7.62 (d, 1 H), 7.54 (d, 1 H), 7.41 (d, 1 H), 7.23 (d, 1 H), 7.16-7.06 (m, 3 H), 6.56 (d, 1 H), 6.43 (d, 1 H), 5.30 (s, 2 H), 5.22 (s, 2 H), 4.66 (s, 2 H). Example 141: Preparation of N-((3-chloro-lH-indol-5-yl)methyl)-l-(2,3-difluoro-4-((2-oxopyrazin-l(2 H)- yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
Figure imgf000244_0001
To a solution of l-[2,3-difluoro-4-(2-oxo-2H-pyrazin-l-ylmethyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3- carboxylic acid (100 mg, 0.268 mmol, 1.0 eq) and C-(3-chloro-l H-indol-5-yl)-methylamine (70 mg, 0.322 mmol, 1.2 eq) in DCM (4 mL) was added HATU (123 mg, 0.322 mmol, 1.2 eq) and Et3N (0.11 mL, 0.804 mmol, 3 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 20/ 1) to give N-((3-chloro-l H- indol-5-yl)methyl)- 1 -(2,3-difluoro-4-((2-oxopyrazin- 1 (2H)-yl)methyl)benzyl)-6-oxo- 1 ,6-dihydropyridine-3- carboxamide (60 mg, 42%) as an off-white solid. LRMS (M+H+) m/z calculated 536.1, found 536.1. ¾ NMR (CD3OD, 400 MHz) δ 8.36 (d, 1 H), 8.02 (s, 1 H), 7.95 (dd, 1 H), 7.57 (d, 1 H), 7.48 (s, 1 H), 7.37 (d, 1 H), 7.32 (d, 1 H), 7.22 (s, 1 H), 7.17 (d, 1 H), 7.12-7.02 (m, 2 H), 6.52 (d, 1 H), 5.26 (s, 2 H), 5.18 (s, 2 H), 4.61 (s, 2 H). Example 142: Preparation of N-((3-chloro-l H-pyrrolo[2,3-b]pyridin-5-yl)methyl)-l-(2,3-difluoro- 4-((2- oxopyrazin-l( -yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
Figure imgf000244_0002
To a solution of l-[2,3-difluoro-4-(2-oxo-2H-pyrazin-l-ylmethyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3- carboxylic acid (100 mg, 0.268 mmol, 1.0 eq) and C-(3-chloro-l H-pyrrolo[2,3-b]pyridin-5-yl)-methylamine (72 mg, 0.322 mmol, 1.2 eq) in DCM (4 mL) was added HATU (123 mg, 0.322 mmol, 1.2 eq) and Et3N (0.11 mL, 0.804 mmol, 3 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and
concentrated. The residue was purified by chromatography on a silica gel column (DCM/MeOH = 10/ 1) to give N-((3-chloro-l H-pyrrolo[2,3-b]pyridin-5-yl)methyl)-l -(2,3-difluoro-4-((2-oxopyrazin-l(2H)- yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide (48 mg, 34%) as an off-white solid. LRMS (M+H+) m/z calculated 537.1, found 537.1. Ή NMR (CD3OD, 400 MHz) δ 8.41 (d, 1 H), 8.32 (d, 1 H), 8.06 (d, 1 H), 8.00 (d, 1 H), 7.97 (dd, 1 H), 7.61 (d, 1 H), 7.44 (s, 1 H), 7.41 (d, 1 H), 7.16-7.06 (m, 2 H), 6.56 (d, 1 H), 5.30 (s, 2 H), 5.22 (s, 2 H), 4.68 (s, 2 H).
Example 143: Preparation of 5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6- fluoro-1 H-indol- 5 -yl)methyl)nicotinamide
Figure imgf000245_0001
5 (4 (acetamjdomet yl) 2'3 dif|uorobenzy|) N ^3 c ioro 6 fluoro H \ηφ\ 5 yl)met yl)njcotinamjc|e
Figure imgf000245_0002
To a solution of N-(2,3-difluoro-4-hydroxymethyl-benzyl)-acetamide (500 mg, 2.326 mmol, 1 eq) in CHCI3 (15 mL) was added SOCb (0.21 mL) and the mixture was refluxed under heating for 1 h. The solvent was evaporated and the residue was used for the next step directly.
Figure imgf000245_0003
To a solution of the above compound and boronic acid A (524 mg, 2.894 mmol, 1.2 eq) in dioxane (16 mL) and water (4 mL) was added Pd(PPli3)4 and Na2CC>3 (740 mg, 6.98 mmol, 3 eq). The mixture was stirred at 95 °C for 1 h under nitrogen. The solvent was removed in vacuo and the residue was diluted with water and was extracted with EA. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (PE/EA = 2/1) to give 5-[4-(acetylamino-methyl)-2,3-difluoro- benzyl] -nicotinic acid methyl ester (640 mg, 88%) as a yellow solid.
Figure imgf000246_0001
To a solution of 5-[4-(acetylamino-methyl)-2,3-difluoro-benzyl]-nicotinic acid methyl ester (640 mg, 1.94 mmol, 1.0 eq) in THF (18 mL)/ H20 (2 mL) was added LiOH.H20 (90 mg, 2.14 mmol, 1.1 eq). The mixture was stirred at 40 °C for 30 min and the solvent was removed in vacuo. The residue was diluted with water and the mixture was acidified to pH 5 with 1 N HQ solution. The mixture was extracted with DCM and the combined extracts were concentrated in vacuo. The residue (420 mg, 67%> yield) as yellow solid was directly used without further
Figure imgf000246_0002
To a solution of 5-[4-(acetylamino-methyl)-2,3-difluoro-benzyl]-nicotinic acid (68 mg, 0.214 mmol, 1.0 eq) and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (50 mg, 0.214 mmol, 1.0 eq) in DCM (4 mL) was added HATU (98 mg, 0.257 mmol, 1.2 eq) and Et3N (0.12 mL, 0.856 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 20/ 1) to give 5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6- fluoro-1 H-indol-5-yl)methyl)nicotinamide (52 mg, 50%>) as a white solid. LRMS (M+H+) m/z calculated 501.1, found 501.1. ¾ NMR (CD3OD, 400 MHz) δ 8.87 (d, 1 H), 8.60 (d, 1 H), 8.12 (s, 1 H), 7.54 (d, 1 H), 7.25 (s, 1 H), 7.14 (d, 1 H), 7.11-7.06 (m, 2 H), 4.72 (s, 2 H), 4.41 (s, 2 H), 4.15 (s, 2 H), 1.96 (s, 3 H).
Example 144: Preparation of 5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-lH-indol- 5- yl)methyl)nicotinamide
Figure imgf000246_0003
5 (4 (acetamidomethy|) 2'3 difiuorobenzyij N ((3 chioro 1H indO| 5 yljmethyljnicotinamjde
Figure imgf000247_0001
To a solution of 5-[4-(acetylamino-methyl)-2,3-difluoro-benzyl]-nicotinic acid (68 mg, 0.214 mmol, 1.0 eq) and C-(3-Chloro-l H-indol-5-yl)-methylamine (50 mg, 0.214 mmol, 1.0 eq) in DCM (4 mL) was added HATU (98 mg, 0.257 mmol, 1.2 eq) and Et3N (0.12 mL, 0.856 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 20/ 1) to give 5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-lH- indol-5-yl)methyl)nicotinamide (60 mg, 55%) as a white solid. LRMS (M+H+) m/z calculated 483.1, found 483.1. ¾ NMR (CD3OD, 400 MHz) δ 8.86 (d, 1 H), 8.56 (d, 1 H), 8.09 (s, 1 H), 7.52 (s, 1 H), 7.35 (d, 1 H), 7.24 (s, 1 H), 7.20 (dd, 1 H), 7.09-7.00 (m, 2 H), 4.66 (s, 2 H), 4.39 (s, 2 H), 4.06 (s, 2 H), 1.97 (s, 3 H).
Example 145: Preparation of 5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-lH-pyrrolo [2,3- b]pyridin-5-yl)methyl)nicotinamide
Figure imgf000247_0002
To a solution of 5-[4-(acetylamino-methyl)-2,3-difluoro-benzyl]-nicotinic acid (70 mg, 0.219 mmol, 1.0 eq) and C-(3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)-methylamine (48 mg, 0.219 mmol, 1.0 eq) in DCM (4 mL) was added HATU (100 mg, 0.263 mmol, 1.2 eq) and Et3N (0.12 mL, 0.876 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 10/ 1) to give 5-(4-(acetamidomethyl)-2,3- difluorobenzyl)-N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)nicotinamide (66 mg, 63%) as a white solid. LRMS (M+H+) m/z calculated 484.1, found 484.1. ¾ MR (CD3OD, 400 MHz) δ 8.88 (d, 1 H), 8.61 (d, 1 H), 8.34 (d, 1 H), 8.11 (s, 1 H), 7.01 (d, 1 H), 7.44 (s, 1 H), 7.13-7.05 (m, 2 H), 4.72 (s, 2 H), 4.41 ( H), 4.15 (s, 2 H), 1.96 (s, 3 H).
Example 146: Preparation of 5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((5-chloro-lH-indazol- 3- yl)methyl)nicotinamide
Figure imgf000248_0001
To a solution of 5-[4-(acetylamino-methyl)-2,3-difluoro-benzyl]-nicotinic acid (70 mg, 0.219 mmol, 1.0 eq) and C-(5-chloro-l H-indazol-3-yl)-methylamine (56 mg, 0.219 mmol, 1.0 eq) in DCM (4 mL) was added HATU (100 mg, 0.263 mmol, 1.2 eq) and Et3N (0.15 mL, 1.1 mmol, 5 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 10/ 1) to give 5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-lH- pyrrolo[2,3-b]pyridin-5-yl)methyl)nicotinamide (65 mg, 61%) as a white solid. LRMS (M+H+) m/z calculated 484.1, found 484.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 13.08 (s, 1 H), 9.31 (s, 1 H), 8.90 (s, 1 H), 8.65 (s, 1 H), 8.34 (s, 1 H), 8.04 (s, 1 H), 7.91 (s, 1 H), 7.53 (d, 1 H), 7.34 (d, 1 H), 7.16-7.05 (m, 2 H), 4.78 (s, 2 H), 4.26 (s, 2 H), 4.09 (d, 2 H), 1.84 (s, 3 H).
Example 147: Preparation of 2-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6- fluoro- lH-indol-5 -yl)methyl)pyridi -4-carboxamide
Figure imgf000248_0002
A solution of 2-bromo-isonicotinic acid methyl ester (1.2 g, 5.58 mmol, leq), Me6Sn2 (1.4 mL, 6.70 mmol, 1.2 eq) and Pd(PPh3)4 (324 mg, 0.28 mmol, 0.05 eq) in toluene (28 mL) was heated at 80 °C for 12 h under nitrogen atmosphere. The mixture was cooled to rt and filtered through a pad of celite. The filtrate was concentrated and the resi
Figure imgf000249_0001
To a solution of 2-trimethylstannanyl-isonicotinic acid methyl ester (290 mg, 0.97 mmol, 1.5 eq) and N-(4- chloromethyl-2,3-difluoro-benzyl)-acetamide (150 mg, 0.64 mmol, leq) in dioxane (10 mL) was added PdCb(PPh3)2 (23 mg, 0.032 mmol, 0.05 eq). The mixture was stirred at 90 °C for 2 h under nitrogen. The mixture was cooled to rt and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 20/ 1) to give 2-[4-(acetylamino-methyl)-benzyl]-isonicotinic acid methyl ester (100 mg, 47%) as an off-white solid.
Figure imgf000249_0002
To a solution of 2-[4-(acetylamino-methyl)-benzyl]-isonicotinic acid methyl ester (100 mg, 0.30 mmol, 1.0 eq) in THF (5 mL)/ H2O (1 mL) was added L1OH.H2O (14 mg, 0.33 mmol, 1.1 eq). The mixture was stirred at 40 °C for 30 min and was acidified to pH 5-6 with IN HC1 solution. The mixture was concentrated in vacuo and the residue was direct
Figure imgf000249_0003
To a solution of the above crude product and C-(3-chloro-6-fluoro-lH-indol-5-yl)-methylamine (60 mg, 0.27 mmol, 0.9 eq) in DCM (4 mL) was added HATU (137 mg, 0.36 mmol, 1.2 eq) and Et3N (0.17 mL, 1.2 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 20/ 1) to give 2-(4-(acetamidomethyl)- 2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)pyridine-4-carboxamide (65 mg, 43% yields for 2 steps) as an off white solid. LRMS (M+H+) m/z calculated 501.1, found 501.1. Ή NMR (CD3OD, 400 MHz) δ 8.34 (d, 1 H), 7.45 (s, 1 H), 7.40 (d, 1 H), 7.29 (d, 1 H), 7.01 (s, 1 H), 6.90 (d, 1 H), 6.85- 6.76 (ni, 2 H), 4.47 (s, 2 H), 4.17 (s, 2 H), 4.00 (s, 2 H), 1.75 (s, 3 H).
Example 148: Preparation of 6-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6- fluoro-lH-indol-5- yl)methyl)pyrazine-2-carboxamide
Figure imgf000250_0001
6"(4"(acetamjdomethyl)"2,3"dif|uorobenzyK"N"
"ch|oro"6"f|Cioro"i H"indor5"yl)methyl)pyrazine"2"carboxamicie
Figure imgf000250_0002
A solution of 6-chloro-pyrazine-2-carboxylic acid methyl ester (690 mg, 4.0 mmol, l eq), Me6Sn2 (1.0 mL, 4.82 mmol, 1.2 eq) and Pd(PPh3)4 (231 mg, 0.20 mmol, 0.05 eq) in toluene (20 mL) was heated at 80 °C for 12 h under nitrogen atmosphere. The mixture was cooled to rt and was concentrated. The residue was purified by chromatography on a silica gel column (EAJ PE = 1/5) to give 6-trimethylstannanyl-pyrazine-2-carboxylic acid methyl ester (360 mg, 30%) as a yellow oil.
Figure imgf000250_0003
To a solution of 6-trimethylstannanyl-pyrazine-2-carboxylic acid methyl ester (264 mg, 0.88 mmol, 1.2 eq) and N-(4-chloromethyl-2,3-difluoro-benzyl)-acetamide (170 mg, 0.73 mmol, l eq) in dioxane (10 mL) was added PdCb(PPh3)2 (26 mg, 0.036 mmol, 0.05 eq). The mixture was stirred at 95 °C for 2 h under nitrogen. The mixture was cooled to rt and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 20/ 1) to give 6-[4-(acetylamino-methyl)-benzyl]-pyrazine-2-carboxylic acid methyl ester (132 mg, 52%) as a yellow solid.
Figure imgf000250_0004
To a solution of 6-[4-(acetylamino-methyl)-benzyl]-pyrazine-2-carboxylic acid methyl ester (100 mg, 0.336 mmol, 1.0 eq) in THF (5 mL)/ ¾0 (1 mL) was added LiOH.EhO (16 mg, 0.369 mmol, 1.1 eq). The mixture was stirred at 40 °C for 30 min and was acidified to pH 5~6 with IN HC1 solution. The mixture was concentrated in vacuo and the residue was directly used without further purification.
Figure imgf000251_0001
To a solution of the above crude product and C-(3-chloro-6-fluoro-lH-indol-5-yl)-methylamine (46 mg, 0.194 mmol, 1 eq) in DCM (4 mL) was added HATU (153 mg, 0.403 mmol, 1.2 eq) and Et3N (0.19 mL, 1.344 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 10/ 1) to give 6-(4- (acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)pyrazine-2-carboxamide (70 mg, 64% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 502.1 , found 502.1. ¾ NMR (DMSO-ifc, 300 MHz) δ 11.39 (s, 1 H), 9.13-9.11 (m, 1 H), 9.06 (s, 1 H), 8.79 (s, 1 H), 8.36-8.32 (m, 1 H), 7.50 (d, 1 H), 7.45 (d, 1 H), 7.22 (d, 1 H), 7.20-7.04 (m, 2 H), 4.63 (d, 2 H), 4.30 (s, 2 H), 4.26 (d, 2 H), 1.84 (s, 3 H). Example 149: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((6- (tetrahydrofuran-2- yl)pyridin-3-yl)methyl)nicotinamide
Figure imgf000251_0002
N ii3 c ioro 6 fluoro IH indoi 5 yumethyh 5
((6"(tetrahydrofuran"2"y|)Pyndin"3"y|)methy|)nicdtinamic|e
Figure imgf000251_0003
To a solution of 6-bromo-pyridine-3-carbaldehyde (5.58 g, 30 mmol, 1 eq) and furan-2-boronic acid (3.36 g, 30 mmol, 1 eq) in dioxane (40 mL) and water (10 mL) was added Pd(PPli3)4 (1.73 g, 1.5 mmol, 0.05 eq) and Na2CC>3 (9.54 g, 90 mmol, 3 eq). The mixture was stirred at 95 °C for 2 h under nitrogen. The mixture was allowed to cool to rt The solvent was removed in vacuo and the residue was diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (EA/ PE = 1/ 5) to give 6-furan-2-yl-pyridine-3-carbaldehyde (4.50 g, 86%) as a yellow solid.
Figure imgf000252_0001
To a solution of 6-furan-2-yl-pyridine-3-carbaldehyde (2.2 g, 11.56 mmol, 1 eq) in MeOH (20 mL) was added NaBH4 (1.0 g, 26.59 mmol, 2 eq) portionwise at 0 °C. Then the mixture was stirred at rt for 1 h and diluted with saturated aqueous NH4CI solution. The combined mixture was concentrated to remove MeOH and the resultant aqueous solution was extracted with DCM, dried and concentrated to give (6-furan-2-yl-pyridin-3- yl)-methanol (1.87 g, 93%).
Figure imgf000252_0002
To a solution of (6-furan-2-yl-pyridin-3-yl)-methanol (800 mg, 4.57 mmol, 1 eq) in MeOH (40 mL) was added 10% Pd/C (200 mg). The mixture was stirred at 40 °C for 30 h under the presence of ¾. Pd/C was removed by filtration and the filtrate was concentrated. The residue was purified by chromatography on a silica gel column (EA/ PE = 1/ 1) to give [6-(tetrahydro-furan-2-yl)-pyridin-3-yl] -methanol (540 mg, 66%>) as a colorless oil.
Figure imgf000252_0003
To a solution of [6-(tetrahydro-furan-2-yl)-pyridin-3-yl] -methanol (500 mg, 2.79 mmol, 1 eq) in EA was added HC1/EA solution. The mixture was stirred at rt for 0.5 h and the excess reagent was removed by evaporation. The residue was treated with SOCb (7 mL) and the mixture was heated under reflux for 1 h. The excess reagent was removed in vacuo. To a solution of the above residue and boronic acid A (2.8 mmol, 1 eq) in dioxane (14 mL) and water (3.5 mL) was added Na2C03 (1.2 g, 11.2 mmol, 4 eq) and Pd(PPli3)4 (162 mg, 0.14 mmol, 0.05 eq). The mixture was stirred at 95 °C for 2 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (EA/ PE = 1/15) to give 5-[6-(tetrahydro-furan-2-yl)-pyridin-3-ylmethyl]-nicotinic acid methyl ester (690 mg, 83%>) as a yellow solid.
Figure imgf000252_0004
To a solution of 5-[6-(tetrahydro-furan-2-yl)-pyridin-3-ylmethyl]-nicotinic acid methyl ester (100 mg, 0.194 mmol, 1.0 eq) in THF (5 mL)/ H20 (1 mL) was added LiOH.H20 (9 mg, 0.213 mmol, 1.1 eq). The mixture was stirred at 40 °C for 30 min and was acidified to pH 5-6 with IN HC1 solution. The mixture was concentrated in vacuo and the residue was directly used without further purification.
Figure imgf000253_0001
To a solution of the above crude product and C-(3-chloro-6-fluoro-lH-indol-5-yl)-methylamine (46 mg, 0.194 mmol, 1 eq) in DCM (4 mL) was added HATU (89 mg, 0.233 mmol, 1.2 eq) and Et3N (0.11 mL, 0.776 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 10/ 1) to give N-((3-chloro-6-fluoro-lH- indol-5-yl)methyl)-5-((6-(tetrahydrofuran-2-yl)pyridin-3-yl)methyl)nicotinamide (40 mg, 41% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 465.1, found 465.1. ¾ NMR (CD3OD, 400 MHz) δ 8.74 (d, 1 H), 8.48 (d, 1 H), 8.28 (d, 1 H), 7.98 (t, 1 H), 7.55 (dd, 1 H), 7.39 (d, 1 H), 7.30 (d, 1 H), 7.11 (s, 1 H), 7.00 (d, 1 H), 4.81-4.77 (m, 1 H), 4.57 (s, 2 H), 3.98 (s, 2 H), 3.98-3.93 (m, 1 H), 3.84-3.78 (m, 1 H), 2.30-2.21 (m, 1 H), 1.90-1.82 (m, 2 H), 1.77-1.70 (m, 1 H).
Example 150: Preparation of 5-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro- 6-fluoro-lH-indol- 5 -yl)methyl)nicotinamide
Figure imgf000253_0002
5"((6"(acetamjdomethyl)pyndinYyl)methyl)"N^
Figure imgf000253_0003
To a solution of (5-bromo-pyridin-2-yl)-methanol (5 g, 26.6 mmol, 1.0 eq), PI13P (8.38 g, 32.0 mmol, 1.2eq), phthalimide (4.69 g, 32.0 mmol, 1.2 eq) in anhydrous THF under nitrogen was added DIAD (6.46 g, 32.0 mmol, 1.2eq) .The mixture was stirred at rt for 12 h and the excess reagent was removed in vacuo. The mixture was extracted with EA and purified by flash column chromatography on silica gel (PE) to give 2-(5- bromo-pyridin-2-ylmethyl)-isoindole-l,3-dione (8 g, 95%) as white solid.
Figure imgf000254_0001
To a solution of 2-(5-bromo-pyridin-2-ylmethyl)-isoindole-l,3-dione (8 g, 25 mmol, 1.0 eq) in EtOH (50 mL) was added N2 H4.H2O (8 mL). The mixture was stirred at 60 °C for 4 h. The precipitate was removed by filtration. The filtrate was concentrated and the residue was purified by flash column chromatography on silica gel (PE) to give C-(5-bromo -pyridin-2-yl -methylamine (4.7 g, 99%) as an orange solid.
Figure imgf000254_0002
To a solution of compound C-(5-bromo-pyridin-2-yl)-methylamine (4.7 g, 25 mmol, 1 eq) in DCM (100 mL) was added AC2O (5.13 g, 50 mmol, 2 eq) followed by Et3N (5.13 g, 50 mmol, 2eq) at 0 °C under nitrogen. After the addition, the mixture was stirred at rt overnight. The mixture was poured into water and extracted with EA. The organic solution was dried and concentrated. The residue was purified by flash column chromatography on silica gel (EA) to give N-(5-bromo-pyridin-2-ylmethyl)-acetamide (5.29 g, 91.8%) as a white solid.
Figure imgf000254_0003
To a solution of compound N-(5-bromo-pyridin-2-ylmethyl)-acetamide (0.5 g, 2.2 mmol, 1 eq) and B2(Pin)2 (0.67 g, 2.6 mmol, 1.2 eq) in dioxane (25 mL) was added KOAc (0.65g, 6.6 mmol, 3 eq) and
(dppf)PdCl2.CH2Ci2 (90 mg, 0.11 mmol, 0.05 eq). The mixture was stirred at 95 °C under nitrogen for 12 h and cool down, to get N-[5-(4,4,5,5N -[5-(4,4,5,5-tetramethyl-[l,3,2]dioxaborolan-2-yl)-pyridin-2-ylmethyl]- acetamide as a crude compound. To a solution of compound N-[5-(4,4,5,5-tetramethyl-[l, 3,2] dioxaborolan-2- yl) -pyridin-2-ylmethyl]-acetamide and 5-chloromethyl-nicotinic acid methyl ester (0.487 g, 2.2 mmol, 1 eq) in dioxane (20 mL) and water (5 mL) was added Na2C03 (0.7 g, 6.6 mmol, 3 eq), and (dppfJPdCk.CitCh. (90 mg, 0.11 mmol, 0.05 eq). The mixture was stirred at 100 °C for 3 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ CH3OH = 10/1) to give 5-[6-(acetylamino-methyl)-pyridin-3- ylmethyl] -nicotinic acid methyl ester (0.59 g, 91%>) as a yellow oil.
Figure imgf000255_0001
To a solution of compound 5-[6-(acetylamino-methyl)-pyridin-3-ylmethyl] -nicotinic acid methyl ester (2.37 g, 7.9 mmol, 1 eq) in THF (90 mL) and water (10 mL) was added LiOH (0.366 g, 8.7 mmol, 1.1 eq). The mixture was stirred at 40 °C for 3 h and was acidified to pH 5-6 with IN HCl solution. The mixture was concentrated in vacuo and
Figure imgf000255_0002
To a solution of compound 5-[6-(acetylamino-methyl)-pyridin-3-ylmethyl]-nicotinic acid (60 mg, 0.21 mmol, 1 eq) and C-(3-chloro-6-fluoro-l H-indol-5-yl) - methylamine (50 mg, 0.21 mmol, 1 eq) in DMF (4 mL) was added HATU (88 mg, 0.231 mmol, 1.1 eq) and Et3N (0.1 mL, 0.84 mmol, 4 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by
chromatography on a silica gel column (DCM/ CH3OH = 10/1) to give 5-((6-(acetamidomethyl)pyridin-3- yl)methyl)-N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)nicotinamide (10 mg, 10%) as an off white solid. LRMS (M+H+) m/z calculated 466.1, found 466.1. !H NMR (DMSO-ifc, 400 MHz) δ 13.1(s, 1 H) 9.3 (t, 1 H), 8.9 (s, 1 H), 8.6 (s, 1 H), 8.5 (s, 1 H), 8.36 (t, 1 H), 8.1 (s, 1 H),7.9 (s, 1 H), 7.6 (d, 1 H), 7.5 (d, 1 H), 7.3 (d, 1 H), 7.2 (d, 1 H), 4.8 (d, 2 H), 4.3 (d, 2 H), 4.0 (s, 2 H), 1.9 (s, 3 H).
Example 151: Preparation of 5-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide
Figure imgf000255_0003
5 ((6 (acetamjdomethyl)pyridin 3 yl)methy|) N ((5 chioro I H indazoi 3 y^methyl^njcotinamide
Figure imgf000256_0001
To a solution of compound 5-[6-(acetylamino-methyl)-pyridin-3-ylmethyl]-nicotinic acid (131 mg, 0.46 mol,l eq) and C-(5-chloro-l H-indazol-3-yl)-methylamine (100 mg, 0.46 mmol, 1 eq) in DMF (4 mL) was added HOBT (75 mg, 0.552 mmol, 1.2 eq), EDCI (106 mg, 0.552 mmol, 1.2 eq) and Et3N (0.186 g, 1.84 mmol, 4 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ CH3OH = 10/1) to give 5-((6- (acetamidomethyl)pyridin-3-yl)methyl)-N-((5-chloro-l H-indazol-3-yl)methyl)nicotinamide (16 mg, 8%) as an off white solid. LRMS (M+H+) m z calculated 449.1, found 449.1. ¾ NMR (CDCI3, 400 MHz) δ 8.8 (d, 1 H), 8.6 (d, 1 H), 8.4 (d, 1 H), 8.2 (s, 1 H), 7.9 (t, 1 H), 7.6 (s, 1 H), 7.45 (m, 1 H), 7.36-7.34 (d, 1 H), 7.25-7.2 (m, 2 H), 4.75-4.74 (d, 2 H), 4.52-4.51 (d, 2 H), 4.02 (s, 1 H), 2.06 (s, 3 H).
Example 152: Preparation of 5-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000256_0002
5 (acetamjdomethyl)pyridin 3 yljmethylj N ((3 chioro IH jndoi 5 y^methyl^njcotinamide
Figure imgf000256_0003
To a solution of 5-[6-(acetylamino-methyl)-pyridin-3-ylmethyl] (131mg, 0.46 mmol, 1 eq) and C-(3-chloro-l H-indol-5-yl)-methylamine (100 mg, 0.46 mmol, 1 eq) in DMF (4 mL) was added HOBT (75 mg, 0.552 mmol, 1.2 eq), EDCI (106 mg, 0.552 mmol, 1.2 eq) and Et3N (0.186 g, 1.84 mmol, 4 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ CH3OH = 10/1) to give 5-[6-(acetylamino -methyl)-pyridin-3- ylmethyl]-N-(3 -chioro- 1 H-indol-5-ylmethyl)-nicotinamide (52 mg, 25% yield) as an off-white solid. LRMS (M+H+) m/z calculated 448.1, found 448.1. ¾ NMR (CDC13, 400 MHz) δ 8.8 (d, 1 H), 8.6 (d, 1 H), 8.4 (d, 1 H), 8.2 (s, 1 H), 7.9 (t, 1 H), 7.6 (s, 1 H), 7.45 (m, 1 H), 7.36-7.34 (d, 1 H), 7.25-7.24 (m, 2 H), 4.75-4.74 (m, 2 H), 4.52-4.51 (m, 2 H), 4.02 (s, 1 H), 2.06 (s, 3 H). Example 153: Preparation of 5-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-lH-pyrrolo [2,3- b]pyridin-5-yl)methyl)nicotinamide
Figure imgf000257_0001
To a solution of 5-[6-(acetylamino-methyl)-pyridin-3-ylmethyl] (131 mg, 0.46 mmol, 1 eq) and C-(3-chloro-l H-pyrrolo[2,3-b]pyridin-5-yl)-methylamine (100 mg, 0.46 mmol, 1 eq) in DMF (4 mL) was added HOBT (75 mg, 0.552 mmol, 1.2 eq), EDCI (106 mg, 0.552 mmol, 1.2 eq) and Et3N (0.186 g, 1.84 mmol, 4 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ CH3OH = 10/1) to give 5-[6-(acetylamino-methyl)- pyridin-3-ylmethyl]-N-(3-chloro-lH-pyrrolo[2,3-b]pyridin-5-ylmethyl)-nicotinamide (42 mg, 20%) as an off white solid. LRMS (M+H+) m/z calculated 449.1, found 449.1. ¾ NMR (DMSO-</6, 400 MHz) δ 11.9 (s, 1 H), 9.2 (t, 1 H), 8.9 (d, 1 H), 8.7 (d, 1 H), 8.5 (s, 1 H), 8.4 (t, 1 H), 8.3 (d, 1 H), 8.1 (s, 1 H), 7.9 (s, 1 H), 7.67 (d, 1 H), 7.65-7.62 (m, 1 H), 7.21 (d, 1 H), 4.59 (d, 2 H), 4.29 (d, 2 H), 4.0 (s, 2 H), 1.9 (s, 3 H).
Example 154: Preparation of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-lH-indol- 5 yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
Figure imgf000257_0002
l"((6"(acetamjdomet y|)pyridinYyKmet y^
carboxamjde 1 NaN3'DMF'RT
2 Pd/C'H2'RT
To a solution of methyl 6-(bromomethyl)nicotinate (9.0 g, 39.12 mmol, 1.0 eq) in DMF (60 mL) was added
NaN3 (3.8 g, 58.68 mmol, 1.5 eq). The resultant mixture was stirred overnight at room temperature. The reaction mixture was added water and extracted with DCM, and the organic lay was dried over Na2SO i, filtered, and concentrated. The residue was dissolved in MeOH, and 10% of Pd/C (500 mg) was added. The resultant mixture was stirred overnight at room temperature under ¾ (1 arm.). The reaction mixture was concentrated. The residue was purified by column chromatography (DCM/ MeOH = 10/1) to give methyl 6-
(aminomethyl)nicotinate (3.0 g, 46%) as a brown solid.
Figure imgf000258_0001
To a solution of methyl 6-(aminomethyl)nicotinate (3.0 g, 18.05 mmol, 1.0 eq) in DCM (30 mL) was added TEA (3.7 g, 36.11 mmol, 2.0 eq). The reaction mixture was cooled to 0 °C, and then AC2O (3.7 g, 36.11 mmol, 2.0 eq) was added dropwise over period of 5 min. The resultant mixture was warmed to rt and stirred for 1 h and the reaction mixture was concentrated. The residue was purified by column chromatography (DCM/ MeOH = 20/ 1) to g do methyl)nicotinate (3.6 g, 95%>) as a yellow solid.
Figure imgf000258_0002
To a solution of methyl 6-(acetamidomethyl)nicotinate (3.6 g, 17.29 mmol, 1.0 eq) in MeOH (50 mL) was added NaBH4 (1.3 g, 34.58 mmol, 2.0 eq) at 0 °C and then the reaction mixture was warmed to rt and kept stirring overnight. The reaction mixture was concentrated and the residue was purified by column (DCM/ MeOH = 10/ 1) to give N-((5-(hydroxymethyl)pyridine -2-yl)methyl)acetamide (2.2 g, 71%) as a yellow solid.
Figure imgf000258_0003
To a stirred solution of N-((5-(hydroxymethyl)pyridin-2-yl)methyl)acetamide (2.2 g, 12.21 mol, 1.0 eq) in DCM (100 mL) was added PPh3 (4.8 g, 18.31 mmol, 1.5 eq) followed by NBS (2.6 g, 14.65 mmol, 1.2 eq) at 0 °C under N2. The reaction mixture was warmed to room temperature and kept stirring for 1 h. The reaction was quenched with water (100 mL). The organic phase was separated, dried over Na2SO i, filtered, and concentrated. The residue was purified by column chromatography (DCM/ MeOH = 15/ 1) to give N-((5- (bromomethyl)pyridin-2-yl)methyl)acetamide (1.7 g, 57%) as a pink solid. LRMS (M+H+) m/z calculated 242, 244, found 243, 245 [M+H] +.
Figure imgf000259_0001
To a solution of methyl 6-hydroxynicotinate (0.63 g, 4.11 mmol, 1.0 eq) in acetone (50 mL) was added K2CO3 (1.1 g, 8.22 mmol, 2.0 eq) followed by N-((5-(bromomethyl)pyridin-2-yl) methyl)acetamide (1.0 g, 4.11 mmol, 1.0 eq). The resultant mixture was heated to reflux and kept stirring overnight. The reaction mixture was concentrated and the residue was purified by column chromatography (DCM/ MeOH = 15/ 1) to give methyl l -((6-(acetamidomethyl)pyridin-3- yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxylate (1.1 g, 85%) as a pink solid .
Figure imgf000259_0002
To a solution of methyl l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-6-oxo-l,6-dihydro pyridine-3- carboxylate (1.0 g, 3.17 mmol, 1.0 eq) in THF/water (5 mL/5 mL) was added L1OH.H2O (0.2 g, 4.76 mmol, 1.5 eq). The resultant mixture kept stirring for 2 h. LRMS showed the SM was consumed and the organic solvent was removed. The reaction mixture was acidified to pH 5 by HC1 (1 N), and then the mixture were concentrated to give the crude l-((6-(acetamidomethyl)pyridin-3- yl)methyl)-6-oxo-l,6-dihydropyridine-3- carboxylic acid (1.4 g) as a pink solid, which was used directly without further purification.
Figure imgf000259_0003
To a solution of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-6-oxo-l,6-dihydropyridine -3-carboxylic acid (140 mg, crude) in DMSO (10 mL) was added (3-chloro-l H-indol-5-yl)methanamine hydrochloride (72 mg, 0.33 mmol, 1.0 eq) followed by EDCI (76 mg, 0.40 mmol, 1.2 eq), HOBT (54 mg, 0.40 mmol, 1.2 eq) and TEA (100 mg, 0.99 mmol, 3.0 eq). The reaction mixture was heated to 30 °C and kept stirring for overnight. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by column chromatography (DCM/ MeOH = 10/ 1) to give l -((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-l H-indol-5-yl)methyl)-6-oxo-l,6- dihydropyridine-3-carboxamide (25 mg, 16%) as a white solid. LRMS (M+H+) m/z calculated 464.1 found/ 464.1 [M+H]+. 'H NMR (DMSO-ifc, 400 MHz) δ 1.86 (s, 3 H), 4.29 (d, 2 H), 4.52 (d, 2 H), 5.16 (s, 2 H), 6.45 (d, 1 H), 7.14 (d, 1 H), 7.25(d, 1 H), 7.36 (d, 1 H), 7.41 (s, 1 H), 7.49 (d, 1 H), 7.70(d, 1 H), 7.92 (d, 1 H), 8.39 (t, 1 H), 8.53 (d, 1 H), 8.80 (t, 1 H), 11.32 (s, 1 H).
Example 155: Preparation of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-lH-pyrrolo[2,3- b]pyridin-5-yl)methyl)-6-oxo-l,6-dihydropyri
Figure imgf000260_0001
Figure imgf000260_0002
To a solution of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-6-oxo-l,6-dihydropyridine -3-carboxylic acid (140 mg, crude) in DMSO (10 mL) was added (3-chloro-l H-pyrrolo[2,3-b]pyridine -5-yl)methanamine hydrochloride (72 mg, 0.33 mmol, 1.0 eq) followed by EDCI (76 mg, 0.40 mmol, 1.2 eq), HOBT (54 mg, 0.40 mmol, 1.2 eq) and TEA (100 mg, 0.99 mmol, 3.0 eq). The reaction mixture was heated to 30 °C and kept stirring for overnight. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by column chromatography (DCM/ MeOH = 10/ 1) to give l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro- lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide (32 mg, 8%) as a white solid. LRMS (M+H+) m/z calculated 465.1, found 465.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 1.86 (s, 3 H), 4.29 (d, 2 H), 4.54 (d, 2 H), 5.15 (s, 2 H), 6.44 (d, 1 H), 7.24 (d, 1 H), 7.67-7.70 (m, 2 H), 7.86-7.92 (m, 2 H), 8.29(d, 1 H), 8.40 (t, 1 H), 8.54 (d, 1 H), 8.85 (t, 1 H), 11.95 (s, 1 H). Example 156: Preparation of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((5-chloro-l H-indazol- 3- yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
Figure imgf000261_0001
l" 6"(acetamidomet yl)Pyridin"3 l)methyl)"N"((5"c loro"iH"indazor3"y|)met yl^
car oxamjde
Figure imgf000261_0002
To a solution of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-6-oxo-l,6-dihydropyridine -3-carboxylic acid (140 mg, crude) in DMSO (10 mL) was added (5-chloro-l H-indazol-3-yl)methanamine hydrochloride (72 mg, 0.33 mmol, 1.0 eq) followed by EDCI (76 mg, 0.40 mmol, 1.2 eq), HOBT (54 mg, 0.40 mmol, 1.2 eq) and TEA (100 mg, 0.99 mmol, 3.0 eq). The reaction mixture was heated to 30 °C and kept stirring for overnight. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2S04, filtered, concentrated. The residue was purified by column chromatography (DCM/ MeOH = 10/ l) to give l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3-yl)methyl)- 6-oxo-l, 6-dihydropyridine-3-carboxamide (12 mg, 8%) as a white solid. LRMS (M+H+) m/z calculated 465.1, found 465.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 1.86 (s, 3 H), 4.28 (d, 2 H), 4.74 (d, 2 H), 5.15 (s, 2 H), 6.44 (d, 2 H), 7.24 (d, 1 H), 7.34 (d, 1 H), 7.53 (d, 1 H), 7.69 (d, 1 H), 7.90-7.93 (m, 2 H), 8.42 (t, 1 H), 8.52 (d, 1 H), 8.57 (d, 1 H), 8.92 (t, 1 H),13.09 (s, 1 H). Example 157: Preparation of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((6-fluoro-l H-indol-5-yl) methyl)-6-oxo-l,6-dihydro ridine-3-carboxamide
1 6 acetamjdo ydi"opyridine 3
Figure imgf000261_0003
To a solution of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-6-oxo-l,6-dihydropyridine -3-carboxylic acid (300 mg, crude) in DMSO (20 mL) was added (6-fluoro-lH-indol-5-yl)meth anamine (164 mg, 1.00 mmol, 1.0 eq) followed by EDCI (228 mg, 1.20 mmol, 1.2 eq), HOBT (162 mg, 1.2 mmol, 1.2 eq) and TEA (304 mg, 3.00 mmol, 3.0 eq). The reaction mixture was purified by prep-HPLC to give l-((6- (acetamidomethyl)pyridin-3-yl)methyl)-N-((6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l,6-dihydropyridine-3- carboxamide (150 mg, 34%) as a white solid. LRMS (M+H+) m/z calculated 448.1, found 448.1; ¾ NMR (DMSO-ifc, 400 MHz) δ 1.86 (s, 3 H), 4.30 (d, 2 H), 4.52 (d, 2 H), 5.16 (s, 2 H), 6.40-6.46(m, 2 H), 7.16 (d, 1 H), 7.25(d, 1 H), 7.31 (d, 1 H), 7.49 (d, 1 H), 7.70 (d, 1 H), 7.93(d, 1 H), 8.41(t, 1 H), 8.53-8.57 (m, 2 H), 8.71 (t, 1 H), 11.10 (s, 1 H).
Example 158: Preparation of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-6- fiuoro- lH-indol- 5-yl) methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
Figure imgf000262_0001
1 6 (acetamidomet yhpyridin 3 ykmethyh N i 3 Chioro 4 fiuoro IH mo\ 5 yhmethyh 6
"r6"dihydronyridine"3"ca
Figure imgf000262_0002
To a solution of l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((4-fluoro-lH-indol-5-yl) methyl)-6-oxo-l,6- dihydropyridine-3 -carboxamide (100 mg, crude) in DCM (20 mL) was added NCS (164 mg, 1.00 mmol, 1.0 eq) at 0 °C. LRMS showed the SM was consumed. The reaction mixture was purified by prep-HPLC to give l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l,6- dihydropyridine-3 -carboxamide (12 mg, 15%) as a white solid. LRMS (M+H+) m/z calculated 482.1, found 482.1 ; ' H NMR (DMSO-ifc, 400 MHz) δ 1.86 (s, 3 H), 4.29 (d, 2 H), 4.54 (d, 2 H), 5.15 (s, 2 H), 6.45 (d, 1 H), 7.20-7.26 (m, 2 H), 7.44 (d, 1 H), 7.51 (s, 1 H), 7.69 (d, 1 H), 7.92 (d, 1 H), 8.40 (t, 1 H), 8.52 (d, 1 H), 8.56 (d, 1 H), 8.76 (t, 1 H), 11.39 (t, 1 H).
Example 159: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2- oxooxazolidin-3- yl)methyl)benzyl)nicotinamide
Figure imgf000262_0003
c ioro 6 fiuoro I H indO| 5 yl)methyl) 5 (4 ((2 oxooxazo|idin 3 y|^methy|jbenzy|^nicotinamide
Figure imgf000263_0001
To a mixture of oxazolidin-2-one (1.0 g, 11.48 mmol, 1.0 eq) in DMF (30 mL) was added NaH (60%, 2.3 g, 57.40 mmol, 5 eq) at 0 °C. The resultant mixture was kept stirring for 30 min. The solution of l-bromo-4- (bromomethyl)benzene (2.9 g, 11.48 mmol, 1 eq) in DMF (10 mL) was added and then the reaction mixture was warmed to rt, and kept stirring for 1 h. Water was added, and the mixture was extracted with DCM, and the organic layer was dried over Na2S04, filtered, and concentrated. The residue was purified by column chromatography (PE/ EA = 3/ 1) to give 3-(4-bromobenzyl)oxazolidin-2-one (2.2 g, 76%) as a white solid.
Figure imgf000263_0002
The mixture of 3-(4-bromobenzyl)oxazolidin-2-one (1.0 g, 3.91 mmol, 1.0 eq), (Pin)2B2(1.2 g, 4.69 mmol, 1.2 eq), PdCl2(dppf).DCM (160 mg, 0.20 mmol, 0.05 eq), KOAc (1.1 g, 11.73 mmol, 3.0 eq) in dioxane (50 mL) was heated to 100 °C and kept stirring for 2 h under N2. And then the reaction mixture was cooled to rt Then the compound methyl 5-(chloromethyl)nicotinate (726 mg, 3.91 mmol 1.0 eq), Na2CC>3 (1.2 g, 11.73 mmol, 3.0 eq), PdCh dppf). DCM (160 mg, 0.20 mmol, 0.05 eq) and water (5 mL) was added. The resultant mixture was heated to 100 °C and kept stirring for 2 h under N2. The reaction mixture was concentrated. And the residue was purified by column chromatography (DCM/ MeOH = 30/ 1) to give methyl 5-(4-((2- oxooxazolidin-3-yl)methyl)benzyl)nicotinate (1.0 g, 77%) as a white solid.
Figure imgf000263_0003
To a solution of methyl 5-(4-((2-oxooxazolidin-3-yl)methyl)benzyl)nicotinate (1.0 g, 3.06 mmol, 1.0 eq) in THF/water (10 mL/10 mL) was added LiOH.FhO (193 mg, 4.60 mmol, 1.5 eq) and then the reaction mixture kept stirring for 2 h. The reaction mixture was concentrated to give the crude product 5-(4-((2-oxooxazolidin- 3-yl)methyl)benzyl)
Figure imgf000263_0004
To a solution of 5-(4-((2-oxooxazolidin-3-yl)methyl)benzyl)nicotinic acid (100 mg, crude) in DMSO (10 mL) was added (3-chloro-6-fluoro-l H-indol-5-yl)methanamine hydrochloride (75 mg, 0.32 mmol, 1.0 eq) followed by EDCI (74 mg, 0.38 mmol, 1.2 eq), HOBT (52 mg, 0.38 mmol, 1.2 eq) and TEA (97 mg, 0.96 mmol, 3.0 eq). The reaction mixture was heated to 30 °C and kept stirring overnight. LRMS showed the SM was consumed. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by column
chromatography (DCM/ MeOH = 10/1) to give N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-5-(4-((2- oxooxazolidin-3-yl)methyl)benzyl)nicotinamide (25 mg, 16%) as a white solid. LRMS (M+H+) m/z calculated 493.1, found/ 493.1; ' H NMR (DMSO-ifc, 400 MHz) δ 3.38 (t, 2 H), 4.03 (s, 2 H), 4.23 (t, 2 H), 4.29 (s, 4 H), 4.58 (d, 2 H), 7.20-7.29 (m, 5 H), 7.45 (d, 1 H), 7.50 (s, 1 H), 8.08 (s, 1 H), 8.64 (s, 1 H), 8.89 (s, 1 H), 9.15 (t, 1 H), 11.38 (s, 1 H).
Example 160: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo- l -((6-((2-oxopyrazin-l(2 H)-yl)methyl)pyridin-3-yl)methyl)-l,6-dihy de
Figure imgf000264_0001
N 1(3 chioro 6 fiuoro I H indO| 5 ykmethyh 6 xo 1 ,,6 ιι2 oxopyrazjn -| ,2H) yl)methy|) yi"idin 3 yljmethyh 1 '6
ddiihhyyddirOoppyyir-iiddine 3 carboxamide
Figure imgf000264_0002
To a mixture of (6-methyl-pyridin-3-yl)-methanol (11.0 g, 89.32 mmol, 1.0 eq) in DCM (250 mL) was added PPh3 (35.1 g, 133.98 mmol, 1.5 eq) at 0 °C followed by NBS (17.5 g, 98.25 mmol, 1.1 eq). The resultant mixture kept stirring for 1 h. The reaction mixture was concentrated to 50 mL. Crude 5-bromomethyl-2- methyl-pyridine was used directly for the next step without further urification.
Figure imgf000264_0003
To a solution of 6-hydroxy-nicotinic acid methyl ester (13.6 g, 89.32 mmol, 1.0 eq) in DMF (150 mL) was added K2CO3 (24.7 g, 178.64 mmol, 2 eq).The reaction mixture was stirred for 30 min, and then crude 5- bromomethyl-2-methyl-pyridine was added. The resultant mixture was stirred overnight. Water (300 mL) was added, and the mixture was extracted with DCM. The organic phase was washed with brine and water, dried over Na2S04, filtered, and concentrated. The residue was purified by column (EA) to give l-(6-methyl- pyridin-3-ylmethyl)-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (10 g, 43%) as a yellow oil.
To a solution of l-(6-methyl-pyridin-3-ylmethyl)-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (10.0 g, 38.72 mmol, 1.0 eq) in DCM (l OOmL) was added w-CPBA (8.0 g, 46.46 mmol, 1.2 eq).The resultant mixture was stirred for 2 h. And then sat.NaHC03 was added and the organic layer was dried over Na2SO i, filtered and concentrated. The residue was dissolved in AC2O (50 mL), and the resultant mixture was heated to 130 °C and kept stirring for 30 min. The solvent was removed in vacuo. Sat. NaHC03 was added, and the mixture was extracted with DCM. The organic phase was dried over Na2SO i, filtered, and concentrated. The residue was dissolved in MeOH, and K2CO3 (10.7 g, 77.44 mmol, 2.0 eq) was added, and kept stirring for 2 h. The reaction mixture was concentrated. The residue was purified by column (DCM/ MeOH = 30/ 1) to give 1 - (6-hydroxymethyl-pyridin-3-yl methyl)-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (1.8 g, 19%) as a white solid.
Figure imgf000265_0002
To a solution of l-(6-hydroxymethyl-pyridin-3-ylmethyl)-6-oxo-l,6-dihydro-pyridine-3- carboxylic acid methyl ester (1.0 mg, 3.65 mmol, 1.0 eq) in DCM (50 mL) was added PPh3 (1.1 g, 4.38 mmol, 1.2 eq) followed by NBS (0.65 g, 3.65 mmol, 1.0 eq) in portions at 0 °C. The reaction mixture was kept stirring for 2 h. The reaction mixture was concentrated to 8 mL and the slurry was added to the solution of Pyrazin-2-ol (351 mg, 3.65 mmol, 1.0 eq) and K2CO3 (1.5 g, 10.95 mmol, 3.0 eq) in DMF (30 mL). The resultant mixture was stirred for 2 h. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by column chromatography (DCM/ MeOH = 30/ 1) to give 6-oxo-l-[6-(2-oxo-2 H-pyrazin-l -ylmethyl)-pyridin-3- ylmethyl]-l,6-dihydro-pyridine- s a white solid.
Figure imgf000265_0003
To a solution of 6-oxo-l -[6-(2-oxo-2 H-pyrazin-l-ylmethyl)-pyridin-3-ylmethyl]-l,6- dihydro-pyridine-3- carboxylic acid methyl ester (1.0 g, 2.84 mmol, 1.0 eq) in THF/water (10 mL/10 mL) was added L1OH.H2O (179 mg, 4.26 mmol, 1.5 eq) and then the reaction mixture was kept stirring for 2 h. The reaction mixture was concentrated to give 6-oxo-l-[6-(2-oxo-2 H-pyrazin-1 - ylmethyl)-pyridin-3-ylmethyl]-l,6-dihydro-pyridine-3- carboxylic acid (1.2 g) as
Figure imgf000266_0001
To a solution of 6-oxo-l -[6-(2-oxo-2 H-pyrazin-l-ylmethyl)-pyridin-3-ylmethyl]-l,6- dihydro-pyridine-3- carboxylic acid (100 mg, 0.29 mmol, 1.0 eq) in DMSO (10 mL) was added C-(3-chloro-6-fluoro-l H-indol-5- yl)-methylamine (68 mg, 0.29 mmol, 1.0 eq) followed by EDCI (67 mg, 0.35 mmol, 1.2 eq), HOBT (47 mg, 0.35 mmol, 1.2 eq) and TEA (88 mg, 0.87 mmol, 3.0 eq). The reaction mixture was heated to 30 °C and kept stirring for overnight. LRMS showed SM was consumed. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2SC>4, filtered, and concentrated. The residue was purified by column chromatography (DCM/ MeOH = 10/ 1) to give N-((3-chloro-6-fluoro-l H- indol-5-yl)methyl)-6-oxo-l-((6-((2-oxopyrazin-l(2 H)-yl)methyl)pyridin-3-yl)methyl)-l,6-dihydropyridine-3- carboxamide (25 mg, 17%) as a white solid. LRMS (M+H+) m z calculated 519.1, found 519.1 ; 'HNMR (DMSO-ifc, 400 MHz) δ 4.53 (d, 2 H), 5.15 (s, 4 H), 6.44 (d, 1 H), 7.21 (d, 1 H), 7.31-7.36 (m, 2 H), 7.44 (d, 1 H), 7.50 (s, 1 H), 7.71-7.74 (m, 2 H), 7.92 (d, 1 H), 8.00 (s, 1 H), 8.50 (s, 1 H), 8.55 (s, 1 H), 8.75(t, 1 H), 11.39 (s, 1 H);.
Example 161: Preparation of N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-6-oxo- l-((6-((2-oxopyrrolidin-l- yl)methyl)pyrid
Figure imgf000266_0002
To a solution of l-(6-hydroxymethyl-pyridin-3-ylmethyl)-6-oxo-l,6-dihydro-pyridine-3- carboxylic acid methyl ester (1.0 mg, 3.65 mmol, 1.0 eq) in DCM (50 mL) was added PPh3 (1.1 g, 4.38 mmol, 1.2 eq) followed by NBS (0.65 g, 3.65mmol, 1.0 eq) in portions at 0 °C. The reaction mixture was kept stirring for 2 h. LRMS showed the SM was consumed. The reaction mixture was concentrated to 8 mL and the residue was purified by column to give the crude intermediate bromide. It was added to the solution of pyrrolidin-2-one (310 mg, 3.65 mmol, 1.0 eq) and NaH (60%, 0.73 g, 10.95 mmol, 3.0 eq) in DMF (30 mL). The resultant mixture was stirred for 2 h. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2SO i, filtered, and concentrated. The residue was purified by column chromatography (DCM/ MeOH = 30/ 1) to give 6-oxo-l -[6-(2-oxo-pyrrolidin-l-ylmethyl)-pyridin-3- ylmethyl]-l,6-dihydro-pyri solid.
Figure imgf000267_0001
To a solution of 6-oxo-l -[6-(2-oxo-pyrrolidin-l -ylmethyl)-pyridin-3-ylmethyl]-l,6- dihydro-pyridine-3- carboxylic acid methyl ester (0.5 g, 1.46 mmol, 1.0 eq) in THF/water (5 mL/5mL) was added LiOH.l hO (92 mg, 2.2 mmol, 1.5 eq) and then the reaction mixture was kept stirring for 2 h. The reaction mixture was concentrated to give the crude 6-Oxo-l-[6-(2-oxo-pyrrolidin-l -ylmethyl) -pyridin-3-ylmethyl]-l,6-dihydro- pyridine-3-carboxyli
Figure imgf000267_0002
To a solution of 6-oxo-l -[6-(2-oxo-pyrrolidin-l -ylmethyl) -pyridin-3-ylmethyl]-l ,6- dihydro-pyridine-3- carboxylic acid (100 mg, 0.31 mmol, 1.0 eq) in DMSO (10 mL) was added compound C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (73 mg, 0.31 mmol, 1.0 eq) followed by EDCI (70 mg, 0.37 mmol, 1.2 eq), HOBT (50 mg, 0.37 mmol, 1.2 eq) and TEA (94 mg, 0.93 mmol, 3.0 eq). The reaction mixture was heated to 30 °C and kept stirring for overnight. LCMS showed the SM was consumed. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2SO i, filtered, and concentrated. The residue was purified by prep-HPLC to give N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-6-oxo-l-((6- ((2-oxopyrrolidin-l-yl)methyl)pyridin-3- yl)methyl)-l ,6-dihydropyridine-3-carboxamide (20 mg, 13%>) as a white solid. LRMS (M+H+) m/z calculated 508.1, found 508.1; 1 H NMR (CD3OD, 400 MHz) δ 2.07 (m, 2 H), 2.46 (t, 2 H), 3.43 (t, 2 H), 4.57 (s, 2 H), 4.68 (s, 2 H), 5.26 (s, 2 H), 6.58 (d, 1 H), 7.14 (d, 1 H), 7.25 (s, 1 H) , 7.32 (d, 1 H), 7.52 (d, 1 H), 7.83 (d, 1 H), 8.99(d, 1 H), 8.47 (s, 1 H) , 8.57 (s, 1 H). Example 162: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l-(2,3-difluoro- 4-((2- oxopyrrolidin-l-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide W"((3"c |oro"6"f|uoro"i yl)benzy|)"6"oxo"i ·6'
Figure imgf000268_0001
To a solution of l-(2,3-difluoro-4-hydroxymethyl-benzyl)-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (1.2 g, 3.88 mmol, 1.0 eq) in DCM (20 mL) was added PPh3 (1.22 g, 4.66 mmol, 1.2 eq) and CBr4 (1.55 g, 4.66 mmol, 1.2 eq). The mixture was stirred at rt for 12 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 3/1-1/1) to afford l-(4- bromomethyl-2,3-difluoro-benzyl)-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester as a white solid (400 mg, 29%).
Figure imgf000268_0002
To a solution of l-(4-bromomethyl-2,3-difluoro-benzyl)-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (100 mg, 0.27 mmol, 1.0 eq) in DMF (10 mL) was added NaH (30 mg, 1.35 mmol, 5.0 eq) and 2- pyrrolidone (26 mg, 0.3 mmol, 1.1 eq) . The mixture was stirred at rt for 2 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 3/1-1/1) to afford l-[2,3-difluoro-4-(2-oxo-pyrrolidin-l -ylmethyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester as a white solid (80 mg, 79%).
Figure imgf000268_0003
The mixture of 1 -[2,3 -difluoro-4-(2-oxo-pyrrolidin- 1 -ylmethyl)-benzyl] -6-oxo- 1 ,6-dihydro-pyridine-3 - carboxylic acid methyl ester (80 mg, 0.21 mmol, 1.0 eq) and LiOH EhO (18 mg, 0.43 mmol, 2.0 eq) in THF (5 mL) and water (1 mL) was stirred at rt for 2 h. The reaction mixture was acidified to pH 3 by HC1 (1 N), and extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product l-[2,3-difluoro-4-(2-oxo-pyrrolidin-l-ylmethyl)-benzyl]-6-oxo-l,6- dihydro-pyridine-3-carbo
Figure imgf000269_0001
To a solution of l-[2,3-difluoro-4-(2-oxo-pyrrolidin-l-ylmethyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3- carboxylic acid (80 mg, 0.21 mmol, 1.0 eq) in DMF (5 mL) was added C-(3-chloro-6-fluoro-l H-indol-5-yl)- methylamine (50 mg, 0.21 mmol, 1.0 eq) followed by HATU (96 mg, 0.25 mmol, 1.2 eq) and TEA (106 mg, 1.05 mmol, 5.0 eq). The reaction mixture was stirred overnight at room temperature. LCMS showed the SM was consumed. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by prep-HPLC to give the desired product N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-l-(2,3-difluoro-4-((2-oxopyrrolidin-l- yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide (20 mg) as a white solid. LRMS (M+H+) m/z calculated 543.1, found 543.1. ¾ NMR (CD3OD, 400 MHz): δ 1.89-1.94 (m, 2 H), 2.29 (t, 2 H), 3.24 (t, 2 H), 4.41 (s, 2 H), 4.55 (s,l H), 5.16 (s, 2 H), 6.44 (d, 1 H), 6.94 (m, 2 H), 7.02 (d, 1 H), 7.12(s,l H) , 7.39 (d, 1 H), 7.87 (d, 1 H), 8.28 (s, 1 H).
Example 163: Preparation of l-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6- fluoro-lH -indol- 5-yl)methyl)-l,6-
Figure imgf000269_0002
To a solution of [4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3-difluoro-phenyl]-methanol (10.0 g, 34.7 mmol, 1.0 eq) in DCM (100 mL) was added the PPh3 (10.1 g, 38.2 mmol, 1.1 eq) and CBr4 (12.7 g, 38.2 mmol, 1.1 eq). The reaction mixture was stirred for 2 h at rt Water was added, and the mixture was extracted with CH2CI2. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 10/ 1-5/ 1) to afford (4-bromomethyl-2,3-difluoro-benzyloxy)-fert- butyl-dimethyl-silane as a white solid (4.1 g, 34%).
Figure imgf000270_0001
To a solution of (4-bromomethyl-2,3-difluoro-benzyloxy)-tert-butyl-dimethyl-silane (4.1 g, 11.4 mmol, 1.0 eq) in DMF (50 mL) under N2, potassium phthalimide (17.5 g, 94 mmol, 1.5 eq) was added and the resulting mixture was at rt for 2 h. The mixture was poured into water and extracted with EtOAc (200 mL X 2). The combined organic layers were washed with water and brine, dried over anhydrous Na2S04 and concentrated. The crude product was subject to silica gel chromatography (PE/ EA = 10/ 1-5/ 1) to afford 2-[4-(tert-butyl- dimethyl-silanyloxymethyl)-2,3-difluoro-benzyl]-isoindole-l,3-dione as a white solid (3.6 g, 75.7%).
Figure imgf000270_0002
To a solution of 2-[4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3-difluoro-benzyl]-isoindole-l,3-dione (3.6 g, 8.6 mmol, 1.0 eq) in EtOH (50 mL) was added the N2 H4.H2O (2.2 g, 43.2 mmol, 5.0 eq). The reaction mixture was stirred for 1 h at rt Water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product 4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3- difluoro-benzylamine was used with
Figure imgf000270_0003
To a solution of 4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3-difluoro-benzylamine (2.8 g, 10.0 mmol, 1.0 eq) in DCM (50 mL) was added the Ac20 (2.1 g, 20.0 mmol, 2.0 eq) and Et3N (2.1 g, 20 mmol, 2.0 eq). The reaction mixture was stirred for 1 h at rt Water was added, and the mixture was extracted with DCM. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 5/ 1 - 2/ 1) to afford N-[4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3-difluoro- benzylj-acetamide as a white solid (2.95 g, 90%).
Figure imgf000270_0004
To a solution of N-[4-(tert-butyl-dimethyl-silanyloxymethyl)-2,3-difluoro-benzyl]-acetamide (2.95 g, 9.0 mmol, 1.0 eq) in THF (40 mL) was added the TBAF (4.2 g, 13.5 mmol, 1.5 eq). The reaction mixture was stirred for 3 h at rt The solvent was removed, and water was added. Then the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 5/ 1 - 2/ 1) to afford N-(2,3-difluoro-4-hydroxymethyl-benzyl)- acetamide as a off-white solid (1.1 g, 56.8%).
Figure imgf000271_0001
To a solution of N-(2,3-difluoro-4-hydroxymethyl-benzyl)-acetamide (0.6 g, 2.8 mmol, 1.0 eq) in CHCb (20 mL) was added the SOCb (0.5 g, 4.2 mmol, 1.5 eq). The reaction mixture was stirred for 3 h at 80 °C. The solvent was removed, and water was added. Then the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel
chromatography (PE/ EA = 10/ 1 - 5/ 1) to afford N-(4-chloromethyl-2,3-difluoro-benzyl)-acetamide as a off- white solid (0.5 g, 76.6%).
Figure imgf000271_0002
To a solution of N-(4-chloromethyl-2,3-difluoro-benzyl)-acetamide (150 mg, 0.64 mmol, 1.0 eq) in DMF (5 mL) was added the K2CO3 (177 mg, 1.28 mmol, 2.0 eq) and 6-hydroxy-nicotinic acid methyl ester (117 mg, 0.77 mmol, 1.2 eq). The reaction mixture was stirred for 3 h at 35 °C. Water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 3/ 1 - 1/ 1) to afford l-[4-(acetylamino-methyl)- 2,3-difluoro-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester as an off-white solid (220 mg, 99%). ¾ NMR (DMSO-ifc, 400 MHz) δ 1.84 (s, 3 H), 3.80 (s, 3 H), 4.26 (d, 2 H), 6.45 (d, 1 H), 6.97 (t, 1 H), 7.08 (t, 1 H), 7.83 (d, 1 H), 8.36 (t, 1 H), 8.65 (s, 1 H).
Figure imgf000271_0003
To a solution of l-[4-(aetylamino-methyl)-2,3-difluoro-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (220 mg, 0.63 mmol, 1.0 eq) in THF (5 mL) and H20 (1 mL) was added the LiOH«H20 (30 mg, 0.7 mmol, 1.1 eq). The reaction mixture was stirred for 1 h at 35 °C. Water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product 1- [4-(acetylamino-methyl)-2,3-difluoro-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid was used without further purification as an
Figure imgf000272_0001
To a solution of l-[4-(acetylamino-methyl)-2,3-difluoro-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid (80 mg, 0.234 mmol, 1.0 eq) in DMF (5 mL) was added C-(3-chloro-6-fluoro-l H-indol-5-yl)- methylamine (50 mg, 0.213 mmol, 1.0 eq) followed by HATU (97 mg, 0.26 mmol, 1.2 eq) and TEA (108 mg, 1.07 mmol, 5.0 eq). The reaction mixture was stirred overnight at room temperature. LCMS showed the SM was consumed. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2SO i, filtered, and concentrated. The residue was purified by prep-HPLC to give 1 - (4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-l,6-dihydro-6- oxopyridine-3-carboxamide (60 mg) as a white solid. LRMS (M+H+) m/z calculated 517.1, found 517.1 ; 1 H NMR (DMSO-ifc, 400 MHz) δ 1.83 (s, 3 H), 4.26 (d, 2 H), 4.54 (d, 2 H), 5.20 (s, 2 H), 6.45 (d, 1 H), 6.96 (t, 1 H),7.08 (d, 1 H), 7.22 (d, 1 H), 7.45 (d, 1 H), 7.50 (d, 1 H), 7.94-7.97 (m, 1 H), 8.35 (t, 1 H), 8.47 (s, 1 H), 8.76 (t, 1 H), 11.38 (s, 1 H).
Example 164: Preparation of 5-(4-(acetamidomethyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)pyridine-3 -carboxamide
Figure imgf000272_0002
To a solution of 1 -bromo-4-bromomethyl-benzene (3.0 g, 12.0 mmol, 1.0 eq) in DMF (40 mL) was added phthalimide potassium (4.4 g, 24.0 mmol, 2.0 eq). The reaction mixture was stirred for 1 h at rt Water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give 2-(4-bromo-benzyl)-isoindole-l,3-dione which was used as a white solid (4.0 g, quant)without further purification.
Figure imgf000273_0001
To a solution of 2-(4-bromo-benzyl)-isoindole-l ,3-dione (4.1 g, 13.1 mmol, 1.0 eq) in EtOH (50 mL) was added the Ν2 Η4Ή2Ο (3.3 g, 65.5 mmol, 5.0 eq). The reaction mixture was stirred for 1 h at rt The solvent was evaporated and water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give 4-bromo-benzylamine which was used without purified as a white solid (2.83 g, quant).
Figure imgf000273_0002
To a solution of 4-bromo-benzylamine (2.83 g, 15.3 mmol, 1.0 eq) in DCM (50 mL) was added the AC2O (2.34 g, 23.0 mmol, 1.5 eq) and Et3N (2.34 g, 23.0 mmol, 1.5 eq). The reaction mixture was stirred for 1 h at rt The solvent was evaporated, and water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 5/ 1 - 2/ 1) to afford N-(4-bromo-benzyl)-acetamide as a white solid (1.5 g, 43%). 1 H NMR (DMSO-ifc, 400 2 H), 7.50 (d, 2 H), 8.36 (s, 1 H).
Figure imgf000273_0003
To a solution of N-(4-bromo-benzyl)-acetamide (1.5 g, 6.6 mmol, 1.0 eq) in dioxane (40 mL) was added 4,4,5,5,4',4',5',5'-octamethyl-[2,2']bi[[l,3,2]dioxaborolanyl] (1.7 g, 6.6 mmol,1.0 eq), Pd(dppf)Cl2CH2Ci2 (0.29 g, 0.66 mmol, 0.1 eq) and AcOK (1.94 g, 19.8 mmol, 3.0 eq). The mixture was stirred at 80 °C for 12 h and allowed to cool to rt Then to this mixture was added 5-chloromethyl-nicotinic acid methyl ester (1.47 g, 6.6 mmol, 1.0 eq), NaCOs (2.12 g, 0.19.8 mmol, 3.0 eq) and H20 (8 mL). The mixture was stirred at 95 °C for 2 h and allowed cool to rt The reaction mixture was extracted with EtOAc (100 mLx3). The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 5/ 1 - 2/ 1) to afford 5-[4-(acetylamino-methyl)-benzyl] -nicotinic acid methyl ester as a yellow solid (1.2 g, 61%).' H NMR (DMSO-ifc, 300 MHz): δ 1.84 (s, 3 H), 3.86 (s, 3 H), 4.04 (s, 2 H), 4.19 (d, 2 H),
7.17-7.25 (m, 4H), 8.09 (t, 2 H), 8.30 (s, 1 H), 8.75 (d, 2 H), 8.91 (d, 2 H).
Figure imgf000274_0001
To a solution of 5-[4-(acetylamino-methyl)-benzyl]-nicotinic acid methyl ester (100 mg, 0.34 mmol, 1.0 eq) in THF (5 mL) and H2O (1 mL) was added the L1OH.H2O (22 mg, 0.51 mmol, 1.5 eq). The reaction mixture was stirred for 1 h at 35 °C. Water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give 5-[4-(acetylamino-methyl)-benzyl]-nicotinic acid was used without purification as a yellow oil (95 mg, 99%).
Figure imgf000274_0002
To a solution of 5-[4-(acetylamino-methyl)-benzyl]-nicotinic acid (95 mg, 0.34 mmol, 1.0 eq) in DMF (5 mL) was added C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (70 mg, 0.31 mmol, 0.9 eq) followed by HATU (155 mg, 0.41 mmol, 1.2 eq) and TEA (172 mg, 1.7 mmol, 5.0 eq). The reaction mixture was stirred overnight at rt. LCMS showed the SM was consumed. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2SO i, filtered, and concentrated. The residue was purified by prep-HPLC to give 5-(4-(acetamidomethyl)benzyl)-N-((3-chloro-6-fluoro-l H-indol-5- yl)methyl)pyridine-3-carboxamide (65 mg, 46%) as a white solid. LRMS (M+H+) m/z calculated 465.1, found 465.1 ; ¾ NMR (DMSO-ifc, 400 MHz) δ 1.83 (s, 3 H), 4.00 (s, 2 H), 4.19 (d, 2 H), 4.58 (d, 2 H), 7.17-7.23 (m,5H), 7.45 (d, 2 H), 7.50 (d, 2 H), 8.05 (s, 1 H), 8.26 (t, 2 H), 8.53 (s, 1 H), 8.88 (s, 1 H), 9.15 (t, 2 H), 11.39 (s, 1 H).
Example 165: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l-((6-((2-oxopyridin -1(2 H)-yl)methyl)pyridin-3-yl)methyl)-l,6- xamide
Figure imgf000274_0003
N ((3 C ioro 6 fiuoro m mo\ 5 yKmet yh 6 oxo 1 ,,2 oxopyrjciin -|,2H) yhmet yh
pyi"idin"3"yl)methyl) 1 '6"di ydi"opyriciine 3'carboxamjde
Figure imgf000275_0001
To a solution of [6-(tetrahydro-furan-2-yl)-pyridin-3-yl] -methanol (1.6 g, 7.40 mmol, 1 eq) in EA was added HCl/EA solution. The mixture was stirred at rt for 0.5 h and the excess reagent was removed by evaporation. The residue was treated with SOCh. (18 mL) and the mixture was heated under reflux for 1 h. The excess reagent was removed in vacuo to give l-(5-chloromethyl-pyridin-2-ylmethyl)-l H-pyridin-2-one as a yellow solid (1.92 g, 96%).
Figure imgf000275_0002
To a solution of l-(5-chloromethyl-pyridin-2-ylmethyl)-l H-pyridin-2-one (500 mg, 1.85 mmol, 1 eq) and 6- oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (284 mg, 1.85 mmol, 1 eq) in acetone (20 mL) was added K2CO3 (1.28 g, 9.25 mmol, 5 eq). The mixture was stirred under reflux for 24 h and the solvent was removed in vacuo. The residue was diluted with water and extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (EA) to give 6-oxo-l -[6-(2-oxo-2 H-pyridin-l -ylmethyl)-pyridin-3-ylmethyl]-l,6-dihydro-pyridine-3-carboxylic acid methyl ester as an off-white solid (100 mg, 15%).
Figure imgf000275_0003
To a solution of 6-oxo-l -[6-(2-oxo-2H-pyridin-l -ylmethyl)-pyridin-3-ylmethyl]-l,6-dihydro-pyridine-3- carboxylic acid methyl ester (100 mg, 0.284 mmol, 1.0 eq) in THF (5 mL)/ H20 (1 mL) was added LiOH.H20 (14 mg, 0.342 mmol, 1.2 eq). The mixture was stirred at 40 °C for 30 min and was acidified to pH 5 with IN HCI solution. The mixture was concentrated in vacuo and the residue was directly used without further purification.
Figure imgf000275_0004
To a solution of the above crude product and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (67 mg, 0.284 mmol, 1 eq) in DCM (4 mL) was added HATU (130 mg, 0.342 mmol, 1.2 eq) and Et3N (0.16 mL, 1.136 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/ MeOH = 10/ 1) to give N-((3-chloro-6-fluoro- 1 H-indol-5-yl)methyl)-6-oxo- 1 -((6-((2-oxopyridin- 1 (2 H)-yl)methyl)pyridin-3 -yl)methyl)- 1 ,6- dihydropyridine-3-carboxamide as a white solid (32 mg, 22% yields for 2 steps). LRMS (M+H+) m/z calculated 518.1, found 518.1 ; Ή NMR (CD3OD, 400 MHz): 8.32 (s, 1 H), 8.20 (s, 1 H), 7.72 (d, 1 H), 7.56- 7.50 (m, 2 H), 7.33-7.27 (m, 2 H), 7.07-7.00 (in, 2 H), 6.89 (d, 1 H), 6.34-6.30 (in, 2 H), 6.18 (t, 1 H), 5.02 (s, 2 H), 4.99 (s, 2 H), 4.23 (s, 2 H).
Example 166: Preparation of N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-l-(2,3-difluoro -4- ((2- oxopyridin- 1 (2 H)-yl)methyl)benzyl)-6-oxo- 1 ,6-dihydropyridine-3 -carboxamide
Figure imgf000276_0001
To a solution of l-(4-bromomethyl-2,3-difluoro-benzyl)-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (160 mg, 0.43 mmol, 1.0 eq) in DMF (10 mL) was added K2C03 (82 mg, 0.87 mmol, 2.0 eq) and pyridin-2-ol (72 mg, 0.52 mmol, 1.2 eq) . The mixture was stirred at rt for 3 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/ EA = 3/ 1 - 1/ 1) to afford l-[2,3-difluoro-4-(2-oxo-2 H-pyridin-l -ylmethyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester as a white solid (110 mg, 66%).
The mixture of l l-[2,3-difluoro-4-(2-oxo-2 H-pyridin-l-ylmethyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3- carboxylic acid methyl ester (110 mg, 0.285 mmol, 1.0 eq) and LiOH EhO (18 mg, 0.43 mmol, 1.5 eq) in THF (5 mL) and water (1 mL) was stirred at rt for 2 h. The reaction mixture was acidified to pH 3 by HC1 (IN), and extracted with ethyl acetate. The organic layer was washed with brine and water, dried over Na2S04 and evaporated to give the crude product l-[2,3-difluoro-4-(2-oxo-2 H-pyridin-l -ylmethyl)-benzyl]-6-oxo-l,6- dihydro-pyridine-3-carbox lic acid (100 mg, quant).
Figure imgf000277_0001
To a solution of l-[2,3-difluoro-4-(2-oxo-2 H-pyridin-l-ylmethyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3- carboxylic acid (100 mg, 0.27 mmol, 1.0 eq) in DMF (5 mL) was added C-(3-chloro-6-fluoro-l H-indol-5-yl)- methylamine (70 mg, 0.30 mmol, 1.1 eq) followed by HATU (125 mg, 0.33 mmol, 1.2 eq) and TEA (164 mg, 1.62 mmol, 6.0 eq). The reaction mixture was stirred overnight at rt. LCMS showed the SM was consumed. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by prep-HPLC to give the desired product N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-l-(2,3-difluoro-4-((2-oxopyridin-l(2 H)-yl)methyl)benzyl)-6- oxo-1, 6-dihydropyridine-3-carboxamide as a white solid (45 mg, 30%). LRMS (M+H+) m/z calculated 553.1, found 553.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 4.54 (d, 2 H), 5.13 (s, 2 H), 5.20 (s, 2 H), 6.25 (t, 1 H), 6.38 (d,l H), 6.43 (d, 1 H), 6.92 (m, 2 H), 7.20 (d, 1 H), 7.44 (m, 2 H), 7.50 (s,l H) , 7.73 (d, 1 H), 7.95 (m, 1 H), 8.47 (s, 1 H), 8.74 (t, 1 H), 11.37 (s, 1 H).
Example 167: Preparation of N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-6-oxo-l -((6-((2-oxopyridin- 1(2 H)-yl) methyl)pyridin-3-yl)methyl)-l ,6-dihydropyridine-3-carboxamide
Figure imgf000277_0002
To a solution of [6-(tetrahydro-furan-2-yl)-pyridin-3-yl] -methanol (1.6 g, 7.40 mmol, 1 eq) in EA was added HCI/EA solution. The mixture was stirred at rt for 0.5 h and the excess reagent was removed by evaporation. The residue was treated with SOCh. (18 mL) and the mixture was heated under reflux for 1 h. The excess reagent was removed in vacuo to give l-(5-chloromethyl-pyridin-2-ylmethyl)-lH-pyridin-2-one (1.92 g, 96%) as a yellow solid.
Figure imgf000278_0001
To a solution of l-(5-chloromethyl-pyridin-2-ylmethyl)-lH-pyridin-2-one (500 mg, 1.85 mmol, 1 eq) and 6- oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (284 mg, 1.85 mmol, 1 eq) in acetone (20 mL) was added K2CO3 (1.28 g, 9.25 mmol, 5 eq). The mixture was stirred under reflux for 24 h and the solvent was removed in vacuo. The residue was diluted with water and extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (EA) to give 6-oxo-l -[6-(2-oxo-2H-pyridin-l -ylmethyl)-pyridin-3-ylmethyl]-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (100 mg, 15%) as an off-white solid.
Figure imgf000278_0002
To a solution of 6-oxo-l -[6-(2-oxo-2 H-pyridin-l-ylmethyl)-pyridin-3-ylmethyl]-l ,6-dihydro-pyridine-3- carboxylic acid methyl ester (100 mg, 0.284 mmol, 1.0 eq) in THF (5 mL)/ H20 (1 mL) was added LiOH.H20 (14 mg, 0.342 mmol, 1.2 eq). The mixture was stirred at 40 °C for 30 min and was acidified to pH 5 with IN HC1 solution. The mixture was concentrated in vacuo and the residue was directly used without further purification. To a solution of the above crude product and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (67 mg, 0.284 mmol, 1 eq) in DCM (4 mL) was added HATU (130 mg, 0.342 mmol, 1.2 eq) and Et3N (0.16 mL, 1.136 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and
concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/MeOH = 10/ 1, v/v) to give N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l -((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)-l,6-dihydropyridine-3-carboxamide (32 mg, 22% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 517.1, found 517.1. 1 H NMR (CD3OD, 400 MHz) δ 8.32 (s, 1 H), 8.20 (s, 1 H), 7.72 (d, 1 H), 7.56-7.50 (m, 2 H), 7.33-7.27 (m, 2 H), 7.07-7.00 (m, 2 H), 6.89 (d, 1 H), 6.34-6.30 (m, 2 H), 6.18 (t, 1 H), 5.02 (s, 2 H), 4.99 (s, 2 H), 4.23 (s, 2 H).
Example 168: Preparation of 5-((6-(l -acetylpyrrolidin-2-yl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-l H- indol-5-yl)methyl)nicotinamide
Figure imgf000279_0001
5-((6-(1 -acetyl pyrrolidin-2-yl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-1H-indol-5-yl)
methyl (nicotinamide
Figure imgf000279_0002
To a solution of 6-bromo-pyridine-3-carbaldehyde (4.4 g, 23.70 mmol, 1 eq) and N-Boc-2-pyrroleboronic acid (5.0 g, 23.70 mmol, 1 eq) in dioxane (160 mL) and water (40 mL) was added Pd(PPh3)4 (1.37 g, 1.19 mmol, 0.05 eq) and Na2CC>3 (7.54 g, 71.10 mmol, 3 eq). The mixture was stirred at 95 °C for 2 h under nitrogen. The mixture was allowed to cool to rt. The solvent was removed in vacuo and the residue was diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (EA/PE = 1/ 5, v/v) to give 2-(5-formyl-pyridin-2-yl)-pyrrole-l -carboxylic acid tert-butyl ester (4.20 g, 66%) as a yellow solid.
Figure imgf000279_0003
To a solution of 2-(5-formyl-pyridin-2-yl)-pyrrole-l -carboxylic acid tert-butyl ester (2.2 g, 8.09 mmol, 1 eq) in MeOH (40 mL) was added NaB¾ (615 mg, 16.18 mmol, 2 eq) portionwise at 0 °C. Then the mixture was stirred at rt for 1 h and diluted with saturated aqueous NH4CI solution. The combined mixture was concentrated to remove MeOH and resultant aqueous solution was extracted with DCM, dried and concentrated to give 2-(5-Hydroxymethyl-pyridin-2-yl)-pyrrole-l-carboxylic acid tert-butyl ester (2.2 g, quant).
Figure imgf000279_0004
To a solution of 2-(5-Hydroxymethyl-pyridin-2-yl)-pyrrole-l-carboxylic acid tert-butyl ester (2.2 g, 8.09 mmol, 1 eq) in MeOH (40 mL) was added 10% Pd/C (600 mg). The mixture was stirred at 50 psi and 40 °C under ¾ for 30 h. Pd/C was removed by filtration and the filtrate was concentrated to give 2-(5- Hydroxymethyl-pyridin-2-yl)-pyrrolidine-l -carboxylic acid tert-butyl ester (2.0 g, 89%>) as a yellow oil.
Figure imgf000280_0001
To a solution of 2-(5-Hydroxymethyl-pyridin-2-yl)-pyrrolidine-l -carboxylic acid tert-butyl ester (400 mg, 1.527 mmol, 1 eq) in EA was added HCl/EA solution. The mixture was stirred at rt for 1 h and was concentrated in vacuo. The residue was re-dissolved in dry DCM (15 mL). To the solution was added AcCI (362 mg, 4.58 mmol, 3 eq) and Et3N (0.73 mL, 6.108 mmol, 4 eq) at 0 °C. The mixture was stirred at 0 °C for 1 h and diluted with water. The organic layer was separated and washed with IN HCI solution and brine. The mixture was dried and concentrated in vacuo.
To a solution of the above crude product in MeOH (15 mL) was added K2CO3 (421 mg, 3.054 mmol, 2 eq). The mixture was stirred at rt for 1 h and concentrated in vacuo. The residue was purified by flash
chromatography on a silica gel column (EA/PE = 1/1, v/v) to give l -[2-(5-Hydroxymethyl-pyridin-2-yl)- pyrrolidin-l-yl]-ethanone (240 mg, 71% for 3 steps) as a yellow oil.
Figure imgf000280_0002
To a solution of l -[2-(5-Hydroxymethyl-pyridin-2-yl)-pyrrolidin-l -yl]-ethanone (240 mg, 1.09 mmol, 1 eq) in EA was added HCl/EA solution. The mixture was stirred at rt for 0.5 h and the excess reagent was removed by evaporation. The residue was treated with SOCb (5 mL) and the mixture was heated under reflux for 1 h. The excess reagent was removed in vacuo. To a solution of the above residue and boroncic acid A (1.64 mmol, 1.5 eq) in dioxane (8.2 mL) and water (2.1 mL) was added Na2C03 (462 mg, 4.36 mmol, 4 eq) and (dppfJPdCb (44 mg, 0.055 mmol, 0.05 eq). The mixture was stirred at 90 °C for 1 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/MeOH = 20/1, v/v) to give 5-[6-(l-acetyl-pyrrolidin-2- yl)-pyridin-3-ylmethyl] -nicotinic acid methyl ester (300 mg, 81% yields for 3 steps) as a brown oil.
Figure imgf000280_0003
To a solution of 5-[6-(l-acetyl-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinic acid methyl ester (300 mg, 0.88 mmol, 1.0 eq) in THF (9 mL)/ H20 (1 mL) was added LiOH.H20 (41 mg, 0.968 mmol, 1.1 eq). The mixture was stirred at 40 °C for 1 h and was acidified to pH 5 with IN HCI solution. The mixture was concentrated in vacuo and the residue was directly used without further purification. To a solution of the above crude product and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (207 mg, 0.88 mmol, 1 eq) in DCM (8 mL) was added HATU (401 mg, 1.056 mmol, 1.2 eq) and Et3N (0.50 mL, 3.52 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/MeOH = 10/1, v/v) to give 5-((6-(l - acetylpyrrolidin-2-yl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)nicotinamide (300 mg, 67% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 506.1, found 506.1. lH NMR (CD3OD, 400 MHz) δ 8.77-8.76 (m, 1 H), 8.52-8.50 (m, 1 H), 8.36 (s, 0.35 H), 8.30 (s, 0.65 H), 8.00 (s, 1 H), 7.61-7.52 (m, 1 H), 7.41 (d, 1 H), 7.14-7.09 (m, 2 H), 7.02 (d, 1 H), 4.96-4.95 (m, 1 H), 4.59 (s, 2 H), 4.03 (s, 0.70 H), 4.00 (s, 1.30 H), 3.75-3.51 (in, 2 H), 2.34-2.19 (m, 1 H), 2.00 (s, 1.95 H), 1.91-1.77 (m, 3 H), 1.67 (s, 1.05 H).
Example 169: Preparation of N-(3-Chloro-6-fluoro-l H-indol-5-ylmethyl)-5-[4-(2-oxo-imidazolidin-l ylmethyl)-benzyl] -nicotinamide
Figure imgf000281_0001
N-(3-Chloro-6-fluoro-1H-indol-5-ylmethyl)-5-[4-(2-oxo
Figure imgf000281_0002
To a solution of l-(4-bromo-benzyl)-imidazolidin-2-one (0.7 g, 2.7 mmol, 1 eq) and B2(Pin)2 (0.84 g, 3.3 mmol, 1.2 eq) in dioxane (25 mL) was added KOAc (0.79g, 8.1mmol, 3 eq) and (dppfjPdCk.CifcCh. (110 mg, 0.11 mmol, 0.05 eq). The mixture was stirred at 95 °C under nitrogen for 2 h and cool down, to yield l-[4- (4,4,5,5-tetramethyl-[l,3,2]dioxaborolan-2-yl)-benzyl]-imidazolidin-2-one as a crude compound. To a solution of compound l-[4-(4,4,5,5-tetramethyl-[l ,3,2]dioxaborolan-2-yl) -benzyl] -imidazolidin-2-one and 5- chloromethyl-nicotinic acid methyl ester (0.6 g, 2.7 mmol, 1 eq) in dioxane (20 mL) and water (5 mL) was added Na2C03 (0.86 g, 8.1 mmol, 3 eq), and (dppf)PdCl2.CH2Cl2 (110 mg, 0.135 mmol, 0.05 eq). The mixture was stirred at 100 °C for 3 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/CH3OH = 10/1, v/v) to give 5-[4-(2-oxo-imidazolidin-l -ylmethyl)-benzyl]-nicotinic acid methyl ester (0.82 g, 92%) as an orange solid.
Figure imgf000282_0001
To a solution of compound 5-[4-(2-oxo-imidazolidin-l-ylmethyl)-benzyl] -nicotinic acid methyl ester (0.82 g, 2.5 mmol, 1 eq) in THF (90 mL) and water (10 mL) was added LiOH (0.138 g, 3.3 mmol, 1.3 eq). The mixture was stirred at 40 °C for 3 h and was acidified to pH 5-6 with IN HC1 solution. The mixture was concentrated in vacuo and the residue was directly used without further purification.
Figure imgf000282_0002
To a solution of compound 5-[4-(2-oxo-imidazolidin-l-ylmethyl)-benzyl] -nicotinic acid (100 mg, 0.3 mmol, 1 eq) and C-(3-chloro-6-fluoro-l H-indol-5-yl) - methylamine (76 mg, 0.3 mmol, 1 eq) in DMF (4 mL) was added HOBT (52 mg, 0.386 mmol, 1.2 eq), EDCl (74 mg, 0.386 mmol, 1.2 eq) and Et3N (0.13 g, 1.3 mmol, 4 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/CH3OH = 10/1, v/v) to give N-(6- fluoro-1 H-indol-5-ylmethyl)-5-[4-(2-oxo-imidazolidin-l-ylmethyl)-benzyl]-nicotinamide (40 mg, 25.3% yield) as an off white solid. LRMS (M+H+) m/z calculated 492.1, found 492.1. ¾ NMR (DMSO-< 6, 400 MHz) δ 9.1 (t, 1 H), 8.9 (d, 1 H), 8.6 (d, 1 H), 8.1 (s, 1 H), 7.5 (s, 1 H), 7.46-7.44 (d, 1 H), 7.3-7.15 (m, 5H), 6.4 (s, 1 H), 4.6 (d, 2 H), 4.18 (s, 2 H), 4.02 (s, 2 H), 3.2-3.1 (m, 5 H).
Example 170: Preparation of l-(4-(acetamidomethyl)benzyl)-N-((3-chloro-6-fluoro-l H-indol-5-yl) methyl)- 6-oxo-l, 6-dihydropyridine-3-carboxamide
Figure imgf000282_0003
1-[4-(Acetylamino-methyl)-benzyl]-6-oxo-1,6-dihydro-pyridine-3-carboxylic acid (3-chloro-6-fluoro-1H-indol-5-ylmethyl)-amide
Figure imgf000282_0004
To a solution of 4-aminomethyl-benzoic acid methyl ester (3.0 g, 14.9 mmol, 1.0 eq) in DCM (50 mL) was added AcCl ( 1.76 g, 22.3 mmol, 1.5 eq) and Et3N (4.51 g, 44.7 mmol, 3.0 eq) . The mixture was stirred at rt for 0.5 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to flash
chromatography (PE/EA = 3/1 - 1/1, v/v) to afford 4-(acetylamino-methyl)-benzoic acid methyl ester as a white solid (2.95 g, 95.8%).
Figure imgf000283_0001
The mixture of 4-(acetylamino-methyl)-benzoic acid methyl ester (2.8 g, 13.5 mmol, 1.0 eq) and LAH (0.62 g, 16.2 mmol, 1.2 eq) in THF (50 mL) was stirred at rt for 1 h. The reaction was quenched with ¾0, and the reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to flash chromatography (PE/EA = 3/1 - 1/1, v/v) to afford N-(4-hydro ellow solid (0.6 g, 25.6%).
Figure imgf000283_0002
To a solution of N-(4-hydroxymethyl-benzyl)-acetamide (600 mg, 3.33 mmol, 1.0 eq) in CHCb (30 mL) was added SOCb (600 mg, 5.0 mmol, 1.5 eq). The reaction mixture was stirred for 1 h at 80 °C. LCMS showed the SM was consumed. The solvent was removed in vacuo. The residue was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and evaporated to give the crude product which was subject to flash chromatography (PE/EA = 5/1 - 2/1, v/v) to afford N-(4-chloromethyl-benzyl)-acetamide as a yellow solid (400 mg, 58%).
Figure imgf000283_0003
To a solution of N-(4-chloromethyl-benzyl)-acetamide (100 mg, 0.48 mmol, 1.0 eq) in DMF (5 mL) was added K2CO3 (133 mg, 0.96 mmol, 2.0 eq) and 6-hydroxy-nicotinic acid methyl ester (88 mg, 0.58 mmol, 1.2 eq). The mixture was stirred at rt for 3 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to flash chromatography (PE/EA = 3/1 - 1/1, v/v) to afford l-[4-(acetylamino-methyl)-benzyl]-6- oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester as a white solid (80 mg, 53%>).
Figure imgf000284_0001
The mixture of l-[4-(acetylamino-methyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid methyl ester (80 mg, 0.255 mmol, 1.0 eq) and LiOH H20 (21 mg, 0.51 mmol, 2.0 eq) in THF (5 mL) and water (1 mL) was stirred at rt for 2 h. The reaction mixture was acidified to pH 3 by HC1 (1 N), and extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product l-[4-(acetylamino-methyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid (70 mg,
93%).
Figure imgf000284_0002
To a solution of -[4-(acetylamino-methyl)-benzyl]-6-oxo-l,6-dihydro-pyridine-3-carboxylic acid (70 mg, 0.255 mmol, 1.0 eq) in DMF (5 mL) was added C-(3-chloro-6-fluoro-lH-indol-5-yl)-methylamine (60 mg, 0.255 mmol, 1.0 eq) followed by HATU (116 mg, 0.306 mmol, 1.2 eq) and TEA (129 mg, 1.275 mmol, 5.0 eq). The reaction mixture was stirred overnight at room temperature. LCMS showed the SM was consumed. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2SC>4, filtered, and concentrated. The residue was purified by prep-HPLC to give the desired product l-[4-(acetylamino-methyl)-benzyl] -6-oxo- l,6-dihydro-pyridine-3-carboxylic acid (3-chloro-6-fluoro-l H- indol-5-ylmethyl)-amide (10 mg) as a white solid. LRMS (M+H+) m/z calculated 481.1, found 481.1. ¾ NMR (CD3OD, 400 MHz) δ 1.95 (s, 3 H), 4.31 (s, 2 H), 4.64 (s, 2 H),5.19 (s, 2 H), 4.55 (d,l H), 7.10 (d, 1 H), 7.22 (s, 1 H), 7.25-7.31 (m, 4 H), 7.48 (d,l H), 7.93-7.96 (m, 1 H) , 8.35 (s, 1 H). Example 171: 5-(4-(acetamidomethyl)-2,5-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5 - yl)methyl)nicotinamide
Figure imgf000284_0003
5-[4-(Acetylamino-methyl)-2,5-difluoro-benzyl]-W-(3-chloro-6-fluoro-1H-indol-5-ylmethyl)-nicotinamide
Figure imgf000284_0004
To a solution of 2,5-difluoro-4-methyl-benzoic acid (5 g, 29.0 mmol, 1.0 eq) in MeOH (200 mL) was added SOCb (17.2 g, 145 mmol, 5 eq). The mixture was stirred at rt for 3 h and the excess reagent was removed in vacuo. The mixture was extracted with EA and purified by flash chromatography on silica gel column (PE/EA = 10/1, v/v) to give 2,5-difluoro-4- 5 g, 83% yield) as colorless oil.
Figure imgf000285_0001
The mixture of 2,5-difluoro-4-methyl-benzoic acid methyl ester (4.0 g, 21.5mmol, 1.0 eq), NBS (4.0g, 22.5 mmol, 1.05 eq) and AIBN (0.088 g, 1.07 mmol, 0.05 eq) in CC14 (100 mL) was stirred at 80 °C overnight. The mixture was poured into water and extracted with CH2CI2. The organic solution was dried and concentrated. The residue was purified by flash column chromatography on silica gel column (PE/EA = 10/1, v/v) to give 4- bromomethyl-2,5-difluoro-benzoic acid methyl ester (4 g, 70.1%) as a yellow solid.
Figure imgf000285_0002
To a solution of 4-bromomethyl-2,5-difluoro-benzoic acid methyl ester (4.0 g, 15.1 mmol, 1 eq) in DMF (150 mL) was added NaNb (1.17 g, 18.12 mmol, 1.2 eq). The mixture was stirred at rt overnight. Then the mixture was poured into water and extracted with EA. The organic solution was dried and concentrated. The residue was purified by flash column chromatography on silica gel column (PE/EA = 10/1, v/v) to give 4- azidomethyl-2,5-difluoro-benzoic acid as a yellow oil.
Figure imgf000285_0003
To a solution of compound 4-azidomethyl-2,5-difluoro-benzoic acid methyl ester (1.8 g, 7.9 mmol, 1 eq) in MeOH (80 mL) was added 10%> Pd/C (700 mg) and the mixture was stirred under a Eh-filled balloon overnight. Pd/C was removed by filtration and the filtrate was concentrated in vacuo. The residue was directly used without further purification
Figure imgf000285_0004
To a solution of compound 4-aminomethyl-2,5-difluoro-benzoic acid methyl ester (1.9 g, 9.4 mmol, 1 eq) and Ac20 (2.9 g, 28.2 mmol, 3 eq) in CH2CI2 (100 mL) was added TEA (1.9 g, 18.8 mmol, 2 eq) and the mixture was stirred at rt overnight. The mixture was then poured into water and extracted with EA. The organic solution was dried and concentrated. The residue was purified by flash column chromatography (PE/EA = 10/1, v/v) to give 4-(acetylamino-methyl)-2,5-difluoro-benzoic acid methyl ester (900 mg, 40%) as a yellow oil.
Figure imgf000286_0001
To a solution of compound 4-(acetylamino-methyl)-2,5-difluoro-benzoic acid methyl ester (900 mg, 3.7 mmol, 1 eq) in THF (40 mL) was added L1AIH4 (562 mg, 16.1 mmol, 4 eq). The mixture was stirred at rt for 4 h. The mixture was poured into water and extracted with CH2CI2. The organic solution was dried and concentrated. The residue was purified by flash column chromatography (PE/EA = 10/1, v/v) to give N-(2,5- difluoro-4 -hydroxy methyl-benzyl) -ac as a yellow solid.
Figure imgf000286_0002
To a solution of N-(2,5-difluoro-4-hydroxymethyl-benzyl)-acetamide (350 mg, 0.93 mmol, 1 eq) in CHCI3 (16 mL) was added SOCb (0.2 mL) and the mixture was refluxed under heating for 1 h. The solvent was evaporated and the crude product without further purification.
Figure imgf000286_0003
To a solution of N-(4-chloromethyl-2,5-difluoro-benzyl)-acetamide(300 mg, 1.28 mmol, 1.0 eq) and boronic acid (345 mg, 1.92 mmol, 1.5 eq) in dioxane (12 mL) and water (3 mL) was added Pd(dppf)Cl2 (52 mg, 0.06 mmol, 0.05 eq) and Na2C03 (545 mg, 5.12 mmol, 4 eq). The mixture was stirred at 95 °C for 1 h under nitrogen. The solvent was removed in vacuo and the residue was diluted with water and was extracted with EA. The combined extracts were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (PE/EA = 2/1) to give 5-[4-(acetylamino-methyl)-2,5-difluoro -benzyl] -nicotinic acid methyl ester (100 mg, 23%>) as a yellow solid
Figure imgf000286_0004
To a solution of 5-[4-(acetylamino-methyl)-2,5-difluoro-benzyl]-nicotinic acid methyl ester (100 mg, 0.29 mmol, 1.0 eq) in THF (4 mL)/ H20 (0.5 mL) was added LiOH.H20 (25 mg, 0.07 mmol, 2.0 eq). The mixture was stirred at 40 °C for 30 min and the solvent was removed in vacuo. The residue was diluted with water and the mixture was acidified to pH 5~6 with IN HC1 solution. The mixture was extracted with DCM and the combined extracts were concentrated in vacuo. The residue (120 mg, yellow solid) was directly used without further purification.
Figure imgf000287_0001
To a solution of 5-[4-(acetylamino-methyl)-2,5-difluoro-benzyl]-nicotinic acid (120 mg, 0.37 mmol, 1.0 eq) and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (96 mg, 0.40 mmol, 1.1 eq) in DCM (4 mL) was added HATU (142 mg, 0.37 mmol, 1.0 eq) and Et3N (0.26 mL, 1.85 mmol, 5 eq). The mixture was stirred at r.t. for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by prep-HPLC to give 5- (4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)nicotinamide (17 mg, 9.2%) as a white solid. LRMS (M+H+) m/z calculated 501.1, found 501.1. ¾ NMR (CD3OD, 400 MHz) δ 8.87 (d, 1 H), 8.60 (d, 1 H), 8.12 (s, 1 H), 7.54 (d, 1 H), 7.25 (s, 1 H), 7.16-7.07 (m, 3 H), 4.72 (s, 2 H), 4.37 (s, 2 H), 4.10 (s, 2 H), 1.99 (s, 3 H).
Example 172: Preparation of l-[4-(Acetylamino-methyl)-2,5-difluoro-benzyl]-6-oxo-l,6 -dihydro-pyridine-3- carboxylic acid (3-chloro-6-fluoro-l H-ind
Figure imgf000287_0002
1-[4-(Acetylaminomet yl)-2,5-difluoro-benzyl]-6-oxo-1 ,6-di ydro-pyridine-3-carboxylic
Figure imgf000287_0003
The mixture of compound 5-[6-(acetylamino-methyl)-pyridin-3-ylmethyl] -nicotinic acid (140 mg, 0.6 mol, 1 eq), 6-oxo-l, 6-dihydro-pyridine-3-carboxylic acid methyl ester and (110 mg, 7.2 mmol, 1.2 eq) and K2CO3 (100 mg, 1.2 mmol, 2.0 eq) in DMF (6 mL) was stirred at rt overnight and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (PE/EA = 2/1) to give 5-[6-(acetylamino-methyl)-pyridin-3-ylmethyl]-N-3-ylmethyl) -nicotinamide (139 mg, 66%) as a yellow solid.
Figure imgf000288_0001
To a solution of l-[4-(acetylamino-methyl)-2,5-difluoro-benzyl]-6-oxo-l,6-dihydro-pyridine- 3-carboxylic acid methyl ester (18.3 mg, 0.39 mmol, 1.0 eq) in THF (4 mL)/ H20 (0.5 mL) was added LiOH.H20 (25 mg, 0.42 mmol, 1.1 eq). The mixture was stirred at 40 °C for 30 min and the solvent was removed in vacuo. The residue was diluted with water and the mixture was acidified to pH 5-6 with 1 N HCl solution. The mixture was extracted with DCM and the combined extracts were concentrated in vacuo. The residue (140 mg, yellow solid) was directly used without further purification.
Figure imgf000288_0002
To a solution of l-[4-(l -[4-(acetylamino-methyl)-2,5-difluoro-benzyl]-6-oxo-l,6-dihydro-pyridine-3- carboxylic acid (140 mg, 0.41 mmol, 1.0 eq) and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (107 mg, 0.45 mmol, 1.1 eq) in DCM (4 mL) was added HATU (157 mg, 0.41 mmol, 1.0 eq) and Et3N (0.22 mL, 1.85 mmol, 1.64 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by prep-HPLC to give acetylamino-methyl)-2,5-difluoro-benzyl]-6-oxo-l,6-dihydro- pyridine-3-carboxylic acid (3-chloro-6-fluoro-l H-indol-5-ylmethyl)-amide (10.3 mg) as a white solid. LRMS
(M+H+) m/z calculated 517.1, found 517.1. 1 H NMR (CD3OD, 400 MHz) δ 8.87 (d, 1 H), 7.76-7.84 (m, 1 H), 7.41 (d, 1 H), 7.12 (s, 1 H), 7.103-6.95 (m, 3 H), 6.45 (d, 1 H), 5.10 (s, 2 H), 4.56 (s, 2 H), 4.25 (s, 2 H), 1.87
(s, 3 H).
Example 173: Preparation of 5-[4-(l-Acetyl-pyrrolidin-2-yl)-3-fluoro-benzyl]-N-(3-chloro-6 -fluoro-1 H- indol-5-ylmethyl)-nicotinamide
Figure imgf000288_0003
5-[4-(1 -Acetyl -pyrrolidin-2-yl)-3-fluoro-benzyl]-N-(3-c loro-6-fluoro-1H-indol-5-ylmet yl)-nicotinamide
Figure imgf000289_0001
To a solution of 4-bromo-3-fluoro-benzaldehyde (2 g, 9.85 mmol, 1 eq) and boronic acid A (2.08 g, 9.85 mmol, 1 eq) in dioxane (100 mL) and water (20 mL) was added Na2CC>3 (3.13 g, 30 mmol, 3 eq), and Pd(PPli3)4 (570 mg, 0.49 mmol, 0.05 eq). The mixture was stirred at 100 °C for 3 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (PE/EA = 10/1, v/v) to give 2-(2- fluoro-4- formyl-phenyl)-pyrrole-l -c solid.
Figure imgf000289_0002
To a solution of 2-(2-fluoro-4-formyl-phenyl)-pyrrole-l-carboxylic acid tert-butyl ester (1.24 g, 4.3 mmol, 1 eq) in MeOH (50 mL) was added NaB¾ (0.32 g, 8.6mmol, 2 eq) portionwise at 0 °C. Then the mixture was stirred at rt for 1 h and diluted with saturated aqueous NH4CI solution. The combined mixture was concentrated to remove MeOH and resultant aqueous solution was extracted with DCM, dried and concentrated to give 2-(2-fluoro-4-hydroxymethyl-phenyl)-pyrrole-l -carboxylic acid tert-butyl ester (1.2 g, 99%) as a black solid. To a solution of 2-(2-fluoro-4-hydroxymethyl-phenyl)-pyrrole-l-carboxylic acid tert- butyl ester (1.2 g, 4.3 mmol, 1 eq) in MeOH (40 mL) was added 10%> Pd/C (370 mg). The mixture was stirred at 60 °C for 30 h under ¾. Pd/C was removed by filtration and the filtrate was concentrated. The residue was purified by flash chromatography on a silica gel column (EA/ PE = 1/ 5, v/v) to give [6-(tetrahydro-furan-2- yl)-pyridin-3-yl]-methanol (1.2 g, 98.4%>) as a colorless oil.
Figure imgf000289_0003
To a solution of [6-(tetrahydro-furan-2-yl)-pyridin-3-yl] -methanol (1.2 g, 4.3 mmol, 1 eq) in EA was added HCI/EA solution. The mixture was stirred at r.t. for 0.5 h and the excess reagent was removed by evaporation. To a solution of the residue in DCM (50 mL) was added TEA(1.3 g, 12.9 mmol, 3 eq) and acetyl
chloride(1.01 g, 12.9 mmol, 3 eq) at 0 °C. Then the mixture was stirred at r.t. for 3 h, and the excess reagent was removed in vacuo. To a solution of the residue in CH3OH (50 mL) was added K2CO3 (1.12 g, 8.14 mmol, 2 eq), and the mixture was stirred at r.t. for 3 h. The residue was purified by flash chromatography on a silica gel column (EA) to give l-[2-(2-fluoro-4-hydroxymethyl-phenyl)-pyrrolidin-l-yl]-ethanone (0.92 g, 56.2%>) as a yellow oil.
Figure imgf000290_0001
To a solution of the above residue and boronic acid (5 mmol, 1 eq) in dioxane (25 mL) and water (5 mL) was added Na2C03 (1.64 g, 20 mmol, 4 eq) and Pd(dppf)Ci2.CH2Ci2 (158 mg, 0.19 mmol, 0.05 eq). The mixture was stirred at 95 °C for 2 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/ CH3OH = 10/1, v/v) to give 5-[4-(l-acetyl-pyrrolidin-2-yl)-3-fluoro-benzyl]-nicotinic acid methyl ester (900 mg, 65%) as a black solid.
Figure imgf000290_0002
T 0 a solution of 5-[4-(l-acetyl-pyrrolidin-2-yl)-3-fluoro-benzyl]-nicotinic acid methyl ester (0.1 g, 2.8 mmol, 1 eq) in THF (90 mL) and water (10 mL) was added LiOH (0.015 g, 3.7 mmol, 1.3 eq). The mixture was stirred at 40 °C for 3 h and was acidified to pH 5~6 with 1 N HC1 solution. The mixture was concentrated in vacuo and the residue was directly used without further purification. To a solution of 5-[4-(l -acetyl - pyrrolidin-2-yl)-3-fluoro-benzyl]-nicotinic acid (100 mg, 0.28 mmol, 1 eq) and C-(3-chloro-6-fluoro-l H- indol-5-yl)-methylamine (66 mg, 0.28 mmol, 1 eq) in DMF (4 mL) was added HATU (117 mg, 0.31 mmol, 1.12 eq), and Et3N (0.13 g, 1.12 mmol, 4 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/CH3OH = 10/1, v/v) to give 5-[4-(l -acetyl-pyrrolidin -2-yl)-3-fluoro-benzyl]-N-(3-chloro-6-fluoro-l H-indol-5-ylmethyl) -nicotinamide (38 mg, 26%) as an off white solid. LRMS (M+H+) m/z calculated 523.1, found 523.1. 1 H NMR (CD3OD, 400 MHz) δ 8.7 (m, 1 H), 8.5 (m, 1 H), 8.0 (m, 1 H), 7.4 (d, 1 H), 7.1 (d, 1 H), 7.0-6.8 (m, 4H), 5.15 (t, 1 H),4.6 (s, 2 H), 4.6 (d, 2 H), 4.0-3.9 (d, 2 H), 3.56-3.51 (m, 2 H), 2.25-2.05 (m, 1 H), 2.0 (s, 2 H),1.9-1.6 (m,5 H). Example 174: Preparation of 5-[4-(l-Acetyl-pyrrolidin-2-yl)-benzyl]-N-(3-chloro-6-fluoro-l H-indol -5- ylmethyl)-nicotinamide
Figure imgf000291_0001
5-[4-(1-Acetyl-pyrrolidin-2-yl)-benzyl]-N-(3-chloro-6-fluoro-1H-indol-5-ylmethyl)-nicotinamide
Figure imgf000291_0002
To a solution of compound 4-bromo-benzaldehyde (4 g, 21.6 mmol, 1 eq) and 1 -tert-butoxycarbonylpyrrol-2- yl boronic acid (5.47 g, 25.9 mmol, 1.2 eq) in dioxane (200 mL) and water (40 mL) was added Na2C03 (6.88 g, 65 mmol, 3 eq), and Pd(PPh3)4 (1.25 g, 1.08 mmol, 0.05 eq). The mixture was stirred at 100 °C for 3 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by falsh chromatography on a silica gel column (PE/EA = 10/1, v/v) to give 2-(4-formyl-phenyl)-pyrrole-l-carboxylic acid tert-butyl ester (4.83 g, 82.4%) as an orange solid.
Figure imgf000291_0003
To a solution of 2-(4-formyl-phenyl)-pyrrole-l -carboxylic acid tert-butyl ester (1.24 g, 4.3 mmol, 1 eq) in MeOH (400 mL) was added NaB¾ (1.35 g, 35.6 mmol, 2 eq) portionwise at 0 °C. Then the mixture was stirred at rt for 1 h and diluted with saturated aqueous NH4CI solution. The combined mixture was concentrated to remove MeOH and resultant aqueous solution was extracted with DCM, dried and concentrated to give 2-(4-hydroxymethyl-phenyl)-pyrrole-l-carboxylic acid tert-butyl ester (4.5 g, 92%) as a black solid. To a solution of 2-(4-hydroxymethyl-phenyl)-pyrrole-l-carboxylic acid tert-butyl ester (4.5g, 16.5 mmol, 1 eq) in MeOH (160 mL) was added 10% Pd/C (1.35 g). The mixture was stirred at 60 °C for 30 h under ¾. Pd/C was removed by filtration and the filtrate was concentrated. The residue was purified on a silica gel column (EA/PE = 1/5, v/v) to give 2-(4-hydroxymethyl-phenyl)-pyrrolidine-l -carboxylic acid tert- butyl ester (4.5 g, 98%>) as a colorless oil.
Figure imgf000291_0004
To a solution of 2-(4-hydroxymethyl-phenyl)-pyrrolidine-l-carboxylic acid tert-butyl ester (4.5g, 16.5 mmol, 1 eq) in EA was added HCI/EA solution. The mixture was stirred at rt for 0.5 h and the excess reagent was removed by evaporation. To a solution of the residue in DCM (200 mL) was added TEA(5 g, 49.5 mmol, 3 eq) and acetyl chloride(3.89 g, 49.5 mmol, 3 eq) at 0 °C. Then the mixture was stirred at r.t. for 3 h, and the excess reagent was removed in vacuo. To a solution of the residue in Cf¾OH (200 mL) was added K2CO3 (4.55 g, 33 mmol, 2 eq), and the mixture was stirred at rt for 3 h. The residue was purified on a silica gel column (EA) to give l-[2-(4-hydroxymethyl-phenyl)-pyrrolidin-l-yl]-ethanone (2.1g, 56.2%) as a yellow oil.
Figure imgf000292_0001
To a solution of l-[2-(4-hydroxymethyl-phenyl)-pyrrolidin-l -yl]-ethanone in CHCI3 (100 mL) was add SOCb (1.3 g) and the mixture was heated under reflux for 1 h. The excess reagent was removed in vacuo. To a solution of the above residue and boronic acid (11.8 mmol, 1 eq) in dioxane (60 mL) and water (15 mL) was added Na2C03 (3.87 g, 36.5 mmol, 4 eq) and Pd(dppf)Cl2.CH2Cl2 (373 mg, 0.46 mmol, 0.05 eq). The mixture was stirred at 95 °C for 2 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/ CH3OH = 10/1, v/v) to give 5-[4-(l-acetyl-pyrrolidin-2-yl)-benzyl]-nicotinic acid methyl ester (1.2 g, 39%) as a black solid.
Figure imgf000292_0002
To a solution of 5-[4-(l -Acetyl-pyrrolidin-2-yl)-benzyl]-nicotinic acid methyl ester (0.1 g, 3 mmol, 1 eq) in THF (90 mL) and water (10 mL) was added LiOH (0.016 g, 3.9 mmol, 1.3 eq). The mixture was stirred at 40 °C for 3 h and was acidified to pH 5-6 with 1 N HCl solution. The mixture was concentrated in vacuo and the residue was directly used without further purification. To a solution of 5-[4-(l-acetyl-pyrrolidin-2-yl)-benzyl]- nicotinic acid (100 mg, 0.28 mmol, 1 eq) and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (66 mg, 0.28 mmol, 1 eq) in DMF (4 mL) was added HATU (120 mg, 0.33 mmol, 1.1 eq), and Et3N (0.13 g, 1.2 mmol, 4 eq). The mixture was stirred at rt for 12 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/CH3OH = 10/1, v/v) to give 5-[4- (l-acetyl-pyrrolidin-2-yl)-benzyl]-N-(3-chloro-6-fluoro-l H-indol-5-ylmethyl)-nicotinamide (40 mg, 26.7%>) as an white solid. LRMS (M+H+) m/z calculated 505.1, found 505.1. 1 H NMR (CD3OD, 400 MHz) δ 8.7 (m, 1 H), 8.45 (m, 1 H), 8.0 (m, 1 H), 7.4(d, 1 H), 7.0-6.8 (m, 6 H), 5.15 (t, 1 H),4.6 (s, 2 H), 4.0-3.9 (d, 2 H), 3.56-3.51 (m, 2 H), 2.25-2.05 (m, 1 H), 2.0 (s,l H), 1.9-1.6 (m,5 H). Example 175: Preparation of 5-(4-(l-acetylpyrrolidin-2-yl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro- 1 H- indol-5-yl)methyl)nicotinamide
Figure imgf000293_0001
5-[4-(1-Acetyl^yrrolidin-2-yl)-2,3-difluoro-benzy
Figure imgf000293_0002
To a solution of 4-bromo-2,3-difluoro-benzaldehyde (4.3 g, 19.5 mmol, 1.0 eq) in dioxane (50 mL) was added boronic acid B (4.11 g, 19.5 mmol, 1.0 eq), Pd(dppf)Cl2CH2Cl2 (1.7 g, 1.95 mmol, 0.1 eq), Na2C03 (6.2 g, 58.5 mmol, 3.0 eq) and H20 (10 mL) . The mixture was stirred at 95 °C for 2 h and allowed cool to rt. The reaction mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to flash chromatography (PE/EA = 5/1-2/1, v/v) to afford 2-(2,3-difluoro- 4-formyl-phenyl)-pyrrole-l-carbo l (2.3 g, 38.3%).
Figure imgf000293_0003
The mixture of 2-(2,3-difluoro-4-formyl-phenyl)-pyrrole-l-carboxylic acid tert-butyl ester (2.3 g, 7.5 mmol, 1.0 eq) and NaBH4 (0.57 g, 15.0 mmol, 2.0 eq) in MeOH (50 mL) was stirred at rt for 2 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product 2-(2,3-difluoro-4-hydroxymethyl-phenyl)-pyrrole-l - carboxylic acid tert-butyl ester as a yellow oil (2.25 g, 98%).
Figure imgf000293_0004
The mixture of 2-(2,3-difluoro-4-hydroxymethyl-phenyl)-pyrrole-l-carboxylic acid tert-butyl ester (2.25 g, 7.28 mmol, 1.0 eq) and Pd/C (0.4 g, 20%) in MeOH (50 mL) was stirred at 60 °C for 30 h under the atmosphere of H2 (60 psi). The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product 2-(2,3-difluoro-4- hydroxymethyl-phenyl)-pyrrolidine-l-carboxylic acid tert-butyl ester as a white solid (2.0 g, 89%>).
Figure imgf000294_0001
The mixture of 2-(2,3-difluoro-4-hydroxymethyl-phenyl)-pyrrolidine-l -carboxylic acid tert-butyl ester (2.0 g, 6.4 mmol, 1.0 eq) in MeOH (50 mL) was added HC1. The mixture was stirred at rt for 1 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product (2,3-difluoro-4-pyrrolidin-2-yl-phenyl)-methanol as a white solid (crude 1.9 g, quant).
Figure imgf000294_0002
The mixture of (2,3-difluoro-4-pyrrolidin-2-yl-phenyl)-methanol (1.9 g, 6.4 mmol, 1.0 eq), AcCI (2.02 g, 25.6 mmol, 4.0 eq) and TEA (2.59 g, 25.6 mmol, 4.0 eq) in DCM (50 mL). The mixture was stirred at rt for 1 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product acetic acid 4-(l -acetyl-pyrrolidin-2-yl)-2,3- difluoro-benzyl ester as a white solid (crude 2.1 g, quant).
Figure imgf000294_0003
To a solution of acetic acid 4-(l-acetyl-pyrrolidin-2-yl)-2,3-difluoro-benzyl ester (2.1 g, 6.4 mmol, 1.0 eq) in MeOH (30 mL) was added K2CO3 (1.77 g, 12.8 mmol, 2.0 eq). The mixture was stirred at rt for 2 h. The reaction mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to flash chromatography (PE/EA = 3/1-1/1, v/v) to afford l-[2-(2,3- difluoro-4-hydroxymethyl-phenyl) oil (1.37 g, 84%).
Figure imgf000294_0004
To a solution of l-[2-(2,3-difluoro-4-hydroxymethyl-phenyl)-pyrrolidin-l -yl]-ethanone (1.37 g, 5.37 mmol, 1.0 eq) inCHCL (30 mL) was added SOC12 (0.96 g, 8.06 mmol, 1.2 eq). The mixture was stirred at 80 °C for 2 h. The reaction mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to flash chromatography (PE/EA = 5/1-3/1, v/v) to afford l-[2-(4- chloromethyl-2,3-difluoro-phenyl)-pyrrolidin-l-yl]-ethanone as a white solid (1.36 g, 92.5%).
Figure imgf000295_0001
To a solution of l-[2-(4-chloromethyl-2,3-difluoro-phenyl)-pyrrolidin-l -yl]-ethanone (0.36 g, 1.31 mmol, 1.0 eq) in dioxane (10 mL) was added 5-(4,4,5,5-tetramethyl-[l,3,2]dioxaborolan-2-yl)-nicotinic acid methyl ester (0.34 g, 1.31 mmol, 1.0 eq), Pd(dppf)Cl2CH2Cl2 (114 mg, 0.13 mmol, 0.1 eq), Na2C03 (417 mg, 3.93 mmol, 3.0 eq) and H20 (2 mL) . The mixture was stirred at 95 °C for 2 h and allowed cool to r.t. The reaction mixture was extracted with EtOAc (100 mL x3). The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to flash chromatography (PE/EA = 5/1 - 1/1) to afford 5-[4-(l -acetyl- pyrrolidin-2-yl)-2,3-difluoro-be l (0.4 g, 81.6%).
Figure imgf000295_0002
To a solution of 5-[4-(l-acetyl-pyrrolidin-2-yl)-2,3-difluoro-benzyl]-nicotinic acid methyl ester (400 mg, 1.07 mmol, 1.0 eq) in THF (5 mL) and H20 (1 mL) was added the LiOH«H20 (90 mg, 2.14 mmol, 2.0 eq). The reaction mixture was stirred for 1 h at rt. Water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give 5-[4-(l-acetyl-pyrrolidin-2-yl)-2,3-difluoro- benzyl] -nicotinic acid as a yellow oil (360 mg, 93.3%), which was used directly without further purification.
Figure imgf000295_0003
To a solution of 5-[4-(l -acetyl-pyrrolidin-2-yl)-2,3-difluoro-benzyl]-nicotinic acid (100 mg, 0.317 mmol, 1.0 eq) in DMF (5 mL) was added C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (70 mg, 0.317 mmol, 1.0 eq) followed by HATU (144 mg, 0.38 mmol, 1.2 eq) and TEA (160 mg, 1.58 mmol, 5.0 eq). The reaction mixture was stirred overnight at r.t. LCMS showed the SM was consumed. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by prep-HPLC to give 5-[4-(l-acetyl-pyrrolidin-2-yl)-2,3-difluoro- benzyl]-N-(3-chloro-6-fluoro-l H-indol-5-ylmethyl)-nicotinamide (60 mg, 35.3%) as a white solid. LRMS (M+H+) m/z calculated 541.1, found 541.1.1 H NMR (DMSO-ifc, 400 MHz) δ 1.69-1.94 (m, 4 H), 2.00 (s, 2 H), 2.18-2.33 (m, 1 H), 3.40-3.75 (m, 2 H), 4.10 (d,2 H), 4.59 (d, 2 H), 5.10-5.24 (m, 1 H), 6.86-6.92 (m, 1 H), 7.04-7.17 (m, 1 H), 7.22 (d, 1 H), 7.45 (d, 1 H), 7.50 (d,l H), 8.08 (s, 1 H). 8.64 (s, 1 H), 8.92 (s,l H), 9.18 (t, 1 H).11.39 (s,l H) Example 176: Preparation of 5-(4-(l-acetylpyrrolidin-2-yl)-2,5-difluorobenzyl)-N-((3-chloro-6-fluoro- 1 H- indol-5-yl)methyl)nicotinamide
Figure imgf000296_0001
To a solution of 4-bromo-2,5-difluoro-benzoic acid (5.0 g, 21.18 mmol, 1 eq) in dry MeOH (70 mL) was added SOCh. (3.1 mL, 42.36 mmol, 2 eq). The mixture was stirred at 70 °C for 3 h and the excess reagent was removed in vacuo. The residue was diluted with EA and washed with saturated NaHC03 solution and brine. The organic phase was dried and concentrated to give 4-bromo-2,5-difluoro-benzoic acid methyl ester (4.7 g, 89%) as a gray solid.
Figure imgf000296_0002
To a solution of 4-bromo-2,5-difluoro-benzoic acid methyl ester (3.23 g, 12.93 mmol, 1 eq) and N-boc-2- pyrroleboronic acid (3.0 g, 14.22 mmol, 1.1 eq) in dioxane (60 mL) and water (15 mL) was added Pd(PPli3)4 (747 mg, 0.647 mmol, 0.05 eq) and Na2C03 (4.12 g, 38.79 mmol, 3 eq). The mixture was stirred at 95 °C for 4 h under nitrogen. The mixture was allowed to cool to rt. The solvent was removed in vacuo and the residue was diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (EA/PE = 1/40, v/v) to give 2-(2,5-difluoro-4-methoxycarbonyl-phenyl)-pyrrole-l - carboxylic acid tert-butyl ester (3.0 g, 69%) as a colorless oil.
Figure imgf000296_0003
To a solution of 2-(2,5-difluoro-4-methoxycarbonyl-phenyl)-pyrrole-l-carboxylic acid tert-butyl ester (3.0 g, 9.34 mmol, 1 eq) in MeOH (50 mL) was added 10% Pd/C (900 mg). The mixture was stirred at 50 psi and 40 °C under ¾ for 24 h. Pd/C was removed by filtration and the filtrate was concentrated in vacuo. The residue was purified by flash chromatography on a silica gel column (EA/PE = 1/20, v/v) to give 2-(2,5-difluoro-4- methoxycarbonyl-phenyl)-pyrrolidine-l-carboxylic acid tert-butyl ester (1.95 g, 64%>) as a colorless oil.
Figure imgf000297_0001
To a solution of 2-(2,5-difluoro-4-methoxycarbonyl-phenyl)-pyrrolidine-l -carboxylic acid tert-butyl ester (1.95 g, 6 mmol, 1 eq) in EA was added HC1/EA solution. The mixture was stirred at rt for 1 h and was concentrated in vacuo. The residue was re-dissolved in dry DCM (60 mL). To the solution was added AcCl (1.3 mL, 18 mmol, 3 eq) and Et3N (3.3 mL, 24 mmol, 4 eq) at 0 °C. The mixture was stirred at 0 °C for 1 h and diluted with water. The organic layer was separated and washed with IN HCI solution and brine. The mixture was dried and concentrated in vacuo to give 4-(l-acetyl-pyrrolidin-2-yl)-2,5-difluoro-benzoic acid methyl ester (1.6 g, 94% for 2 steps) as a brown oil.
Figure imgf000297_0002
To a solution of 4-(l -acetyl-pyrrolidin-2-yl)-2,5-difluoro-benzoic acid methyl ester (1.60 g, 5.65 mmol, 1 eq) in dry THF (50 mL) was added LAH (650 mg, 17 mmol, 3 eq). The mixture was stirred at 0 °C for 1 h and was quenched by the addition of EA. The mixture was concentrated in vacuo and the residue was diluted with water. The mixture was extracted with EA and the combined extracts were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (EA) to give l-[2-(2,5-difluoro-4- hydroxymethyl-phenyl)-pyrr oil.
Figure imgf000297_0003
To a solution of l-[2-(2,5-difluoro-4-hydroxymethyl-phenyl)-pyrrolidin-l -yl]-ethanone (180 mg, 0.70 mmol, 1 eq) in CHC13 (5 mL) was added SOCh. (0.05 mL, 0.85 mmol, 1.2 eq). The mixture was stirred at 60 °C for 1 h and the excess reagent was removed by evaporation.
To a solution of the above residue and boronic acid A (0.85 mmol, 1.2 eq) in dioxane (4.2 mL) and water (1.0 mL) was added Na2C03 (300 mg, 2.82 mmol, 4 eq) and Pd(dppf)Cl2 (29 mg, 0.035 mmol, 0.05 eq). The mixture was stirred at 95 °C for 1 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (EA/PE = 2/1, v/v) to give 5-[4-(l -acetyl-pyrrolidin-2-yl)-2,5-difluoro-benzyl]-nicotinic acid methyl ester (90 mg, 34%> yields for 2 steps) as a yellow solid.
Figure imgf000298_0001
To a solution of 5-[4-(l-acetyl-pyrrolidin-2-yl)-2,5-difluoro-benzyl] -nicotinic acid methyl ester (90 mg, 0.24 mmol, 1.0 eq) in THF (5 mL)/ ¾0 (1 mL) was added L1OH.H2O (12 mg, 0.29 mmol, 1.2 eq). The mixture was stirred at 40 °C for 1 h and was acidified to pH 5 with 1 N HC1 solution. The mixture was concentrated in vacuo and the residue was directly used without further purification. To a solution of the above crude product and C-(3-chloro-6-fluoro-l H-indol-5-yl)-methylamine (57 mg, 0.24 mmol, 1 eq) in DCM (4 mL) was added HATU (92 mg, 0.29 mmol, 1.2 eq) and Et3N (0.13 mL, 0.96 mmol, 4 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/MeOH = 20/1 , v/v) to give 5-(4-(l -acetylpyrrolidin-2-yl)-2,5-difluorobenzyl)-N-((3- chloro-6-fluoro-lH-indol-5-yl)methyl)nicotinamide (90 mg, 69% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 541.1, found 541.1. ¾ NMR (CD3OD, 400 MHz) δ 8.76-8.74 (m, 1 H), 8.50-8.47 (m, 1 H), 8.00 (s, 1 H), 7.41 (d, 1 H), 7.12 (s, 1 H), 7.06-6.93 (m, 2 H), 6.79-6.72 (m, 1 H), 5.15-5.08 (m, 1 H), 4.59 (s, 2 H), 3.99 (s, 1 H), 3.95 (s, 1.3 H), 3.74-3.49 (in, 2 H), 2.32-2.16 (m, 1 H), 2.02 (s, 2 H), 1.90-1.71 (m, 3 H), 1.72 (s, 1 H).
Example 177: Preparation of 5-[4-(acetylamino-methyl)-2-fluoro-benzyl]-N-(3-chloro-6-fluoro-lH-indol-5- ylmethyl)-nicotinamide
Figure imgf000298_0002
To a solution of 4-bromo-3-fluoro-benzaldehyde (10.1 g, 49.8 mmol, 1.0 eq) in MeOH (100 mL) was added NaB¾ (3.8 g, 99.6 mmol, 2.0 eq). The mixture was stirred at rt for 2 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product (4-bromo-3-fluoro-ph -methanol (10.3 g, 100%).
Figure imgf000298_0003
The mixture of (4-bromo-3-fluoro-phenyl)-methanol (10.3 g, 50.0 mmol, 1.0 eq), PPI13 (15.7 g, 60.0 mmol, 1.2 eq) and DIAD (12.1 g, 60.0 mmol, 1.2 eq) in THF (100 mL) was stirred at rt for 12 h. The reaction was quenched with H2O. The reaction mixture was extracted with ethyl acetate, and the organic was washed with brine, dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/EA = 10/1-5/1, v/v) to afford 2-(4-bromo-3-fluoro-benzyl)-isoindole-l,3-dione (16.4 g, 98%) as a white solid.
Figure imgf000299_0001
To a solution of 2-(4-bromo-3-fluoro-benzyl)-isoindole-l,3-dione (5.0 g, 15.0 mmol, 1.0 eq) in EtOH (100 mL) was added Ν2¾ H2O (3.75 g, 75 mmol, 5.0 eq). The reaction mixture was stirred at rt for 1 h. The solvent was removed in vacuo, and the residue was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and evaporated to give the crude product which was subject to silica gel chromatography (PE/EA = 3/1-1/1, v/v) to afford 4-bromo-3-fluoro-benzylamine (1.5 g, 46%) as a yellow oil.
Figure imgf000299_0002
To a solution of 4-bromo-3-fluoro-benzylamine (1.5 g, 7.35 mmol, 1.0 eq) in THF (50 mL) was added B0C2O (3.2 g, 14.7 mmol, 2.0 eq) and DMAP (96 mg, 0.74 mmol, 0.1 eq). The mixture was stirred at rt for 2 h. The reaction mixture was extracted with ethyl acetate, washed with brine and water. The organic layer was dried over Na2S04 and evaporated to give the crude product which was subject to silica gel chromatography (PE/EA = 20/1-10/1 , v/v) to afford (4-bromo-3-fluoro-benzyl)-carbamic acid tert-butyl ester (800 mg, 36%>) as a white solid.
Figure imgf000299_0003
5-[4-(tert-Butoxycarbonylamino-methyl)-2-fluoro-benzyl]-nicotinic acid methyl ester (1.0 g, 41%>) was prepared as described for 5-(6-hydroxy methyl-pyridin-3-ylm acid methyl ester.
Figure imgf000299_0004
(4- {5-[(3-Chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-3-ylmethyl} -3-fluoro-benzyl)-carb acid tert-butyl ester (500 mg, 100%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5- ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl) .
Figure imgf000299_0005
To a solution of (4- {5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-3-ylmethyl}-3-fluoro- benzyl)-carbamic acid tert-butyl ester (500 mg, 0.93 mmol, 1.0 eq) in DCM (20 mL) was added HC1 (80 mg, 2.3 mmol, 2.0 eq). The reaction mixture was stirred at rt for 1 h. Water was added, and the mixture was extracted with EtOAc. The organic layer was dried over Na2S04 and evaporated to give 5-(4-aminomethyl-2- fluoro-benzyl)-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-nicotinamide was used without purification (440 mg, 98%) as a white solid.
Figure imgf000300_0001
To a solution of 5-(4-aminomethyl-2-fluoro-benzyl)-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-nicotinamide (100 mg, 0.23 mmol, 1.0 eq) in DCM (10 mL) was added Et3N (47 mg, 0.46 mmol, 2.0 eq) and Ac20 (100 mg, 0.46 mmol, 2.0 eq). The reaction mixture was stirred at rt for 1 h. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2SC>4, filtered, and
concentrated. The residue was purified by prep-HPLC to give 5-[4-(acetylamino-methyl)-2-fluoro-benzyl]-N- (3-chloro-6-fluoro-lH-indol-5-ylmethyl)-nicotinamide (30 mg, 27%) as a white solid. LRMS (M+H+) m/z calculated 483.1, found 483.1. ¾ NMR (DMSO-tfc, 400 MHz) δ 11.38 (s, 1 H), 9.17 (d, 1 H), 8.90 (d, 1 H), 8.61 (d, 1 H), 8.32 (s, 1 H), 8.02 (s, 1 H), 7.50 (d, 1 H), 7.45 (d, 1 H), 7.31 (t, 1 H), 7.21 (d, 1 H), 7.04 (d, 2 H), 4.58 (s, 2 H), 4.21 (d, 2 H), 4.04 (s, 2 H), 1.85 (s, 3 H).
Example 178: Preparation of N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-{2-fluoro-4-[(2-hydroxy- acetylamino)-methyl] -benzyl} -nicotinamide
Figure imgf000300_0002
To a solution of 5-(4-aminomethyl-2-fluoro-benzyl)-N-(3-chloro-6-fluoro-lH- indol-5-ylmethyl)- nicotinamide (100 mg, 0.23 mmol, 1.0 eq) in DMF (10 mL) was added Et3N (47 mg, 0.46 mmol, 2.0 eq), hydroxy-acetic acid (34 mg, 0.46 mmol, 2.0 eq) and HATU (105 mg, 0.28 mmol, 1.2 eq). The reaction mixture was stirred at rt for 1 h. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by prep- HPLC to give 5- {4-[(2-amino-acetylamino)-methyl]-2-fluoro-benzyl}-N-(3-chloro-6-fluoro-lH-indol-5- ylmethyl)-nicotinamide (30 mg, 27%>) as a white solid. LRMS (M+H+) m/z calculated 498.1, found 498.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.38 (s, 1 H), 9.16 (s, 1 H), 8.90 (d, 1 H), 8.61 (d, 1 H), 8.31(t, 1 H), 8.01 (s, 1 H), 7.50 (s, 1 H), 7.45 (d, 1 H), 7.29 (t, 1 H), 7.22 (d, 2 H), 7.06 (d, 2 H), 5.48 (t, 1 H), 4.58 (s, 2 H), 4.26 (d, 2 H), 4.03 (s, 2 H), 3.84 (d, 2 H).
Example 179: Preparation of
5-{4-[(2-amino-acetylamino)-methyl]-2-fluoro-benzyl} -N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)- nicotinamide
Figure imgf000301_0001
5-{4-[(2-Amino-acetylamino)-methyl]-2-fl
nicotinamide (18 mg, 17%) was prepared as described for 5- {4-[(2-amino-acetylamino)-methyl]-2-fluoro- benzyl}-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-nicotinamide. LRMS (M+H+) m/z calculated 497.1, found 497.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.17 (t, 1 H), 8.90 (d, 1 H), 8.61 (d, 1 H), 8.32 (s, 1 H), 8.02 (s, 1 H), 7.44-7.50 (m, 2 H), 7.06 (d, 2 H), 4.58 (s, 2 H) ,4.26 (d, 2 H), 4.03 (s, 2 H), 3.12 (s, 2 H), 4.03 (s, 2 H).
Example 180: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2- hydroxyacetamido)methyl)benzyl)nicotinamide
Figure imgf000301_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-hydroxyacetamido)methyl)benzyl)nicotinamide (50 mg, 87%) was prepared as described for 5-{4-[(2-amino-acetylamino)-methyl]-2-fluoro-benzyl} -N-(3-chloro- 6-fluoro-lH-indol-5-ylmethyl)-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 481.1, found 481.1. 1H NMR (DMSO-i 6, 400 MHz) δ 11.39 (s, 1 H), 9.16 (t, 1 H), 8.90 (s, 1 H), 8.64 (s, 1 H), 8.21 (t,l H), 15 8.06 (s, 1 H), 7.51 (d, 1 H), 7.47 (d, 1 H), 7.24 (s, 1 H), 7.21 (s, 4 H), 5.45 (t, 1 H), 4.59 (d, 2 H), 4.26 (d, 2 H), 4.01 (s, 2 H), 3.84 (d, 2 H).
Example 181: Preparation of 5-(4-((2-aminoacetamido)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)nicotinamide
Figure imgf000301_0003
5-(4-((2-Aminoacetamido)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)nicotinamide hydrochloride (40 mg, 80%>) was prepared as described for 5-(4-aminomethyl-benzyl)-N-(3-chloro-6-fluoro- lH-indol-5-ylmethyl)-nicotinamide as a light yellow solid. LRMS (M+H+) m/z calculated 480.1, found 480.1 ¾ NMR (DMSO-ifc, 400 MHz) δ 11.48 (s, 1 H), 9.40 (t, 1 H), 9.04 (s, 1 H), 8.86 (t, 1 H), 8.79 (d, 1 H), 8.37 (s, 1 H), 8.11 (s, 3 H), 7.51 (d, 1 H), 7.48 (d, 1 H), 7.25 (s, 5 H) , 4.60 (d, 2 H), 4.29 (d, 4 H), 4.10 (s, 2 H), 3.57 (q, 2 H).
Example 182: Preparation of 5-(4-(acetamidomethyl)-3-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl) nicotinamide
Figure imgf000302_0001
5-(4-(Acetamidomethyl)-3-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)nicotinami (50 mg, 44%) was prepared as described for (5- {5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-3- ylmethyl}-pyridin-2-ylmethyl)-carbamic acid fert-butyl ester as a white solid. LRMS (M+H+) m z calculated 483, found 483. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (br, 1 H), 9.15 (t, 1 H), 8.90 (d, 1 H), 8.65 (d, 1 H), 8.25 (t, 1 H), 8.08 (t, 1 H), 7.50 (d, 1 H), 7.46 (d, 1 H), 7.21-7.26 (m, 2 H), 7.07-7.14 (m, 2 H), 4.59 (d, 2 H), 4.22 (d, 2 H), 4.03 (s, 2 H), 1.83 (s, 3 H).
Example 183: Preparation of
2-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N luoro-lH-indol-5-yl)methyl)isonicotinamide
Figure imgf000302_0002
\ Q
2-((6-(Acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)isonicotinamide 2,2,2-trifluoroacetate (25 mg, 29% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH- indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)- pyridin-3-ylmethyl] -nicotinamide. LRMS (M+H+) m/z calculated 467.1, found 467.1. ¾ NMR (CD3OD, 400 MHz) δ 8.69 (d, 1 H) 8.62 (d, 1 H), 8.38 (d, 1 H), 7.83 15 (s, 1 H), 7.81 (d, 1 H), 7.71 (dd, 1 H), 7.50 (d, 1 H), 7.22 (s, 1 H), 7.11 (d, 1 H), 4.69 (s, 2 H),4.62 (s, 2 H), 4.41 (s, 2 H), 2.03 (s, 3 H).
Example 184: Preparation of methyl 5-(4-(l-acetylpyrrolidin-2-yl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro- 1 H-indol-5 -yl)methyl)nicotinamide
Figure imgf000302_0003
To a solution of 4-bromo-2-fluoro-benzaldehyde (4 g, 19.7 mmol, 1 eq) and N-Boc-2-pyrroleboronic acid (5.4 g, 25.61 mmol, 1.3 eq) in dioxane (160 mL) and water (40 mL) was added Pd(PPh3)4 (1.48 g, 1.28 mmol, 0.05 eq) and Na2C03 (6.26 g, 59.10 mmol, 3 eq). The mixture was stirred at 95 °C for 2 h under nitrogen. The mixture was allowed to cool to rt. The solvent was removed in vacuo and the residue was diluted with EA and 5 water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (EA/PE = 1/5, v/v) to give 2-(3-fluoro-4-formyl-phenyl)-pyrrole-l -carboxylic acid tert-butyl ester (4.4 g, 77%) as a yellow solid.
Figure imgf000303_0001
To a solution of 2-(3-fluoro-4-formyl-phenyl)-pyrrole-l-carboxylic acid tert-butyl ester (4.5 g, 15.60 mmol, 1 eq) in MeOH (150 mL) was added NaB¾ (1.18 g, 31.20 mmol, 2 eq) portionwise at 0 °C. Then the mixture was stirred at rt for 1 h and diluted with saturated aqueous NH4CI solution. The combined mixture was concentrated to remove MeOH and resultant aqueous solution was extracted with DCM, dried and concentrated to give 2-(3-fluoro-4-hydroxymethyl-phenyl)-pyrrole-l -carboxylic acid tert-butyl ester (4 g, quant).
Figure imgf000303_0002
To a solution of 2-(3-fluoro-4-hydroxymethyl-phenyl)-pyrrole-l -carboxylic acid tert-butyl ester (4 g, 13.7 mmol, 1 eq) in MeOH (100 mL) was added 10% Pd/C (3 g). The mixture was stirred at 40 °C under H2 (50 psi) for 30 h. Pd/C was removed by filtration and the filtrate was concentrated to give 2-(3-fluoro-4- hydroxymethyl-phenyl)-pyrrolidine-l-carb (3.5 g, 86%) as a yellow oil.
Figure imgf000303_0003
To a solution of 2-(3-fluoro-4-hydroxymethyl-phenyl)-pyrrolidine-l -carboxylic acid tert-butyl ester (4.7 g, 15.93 mmol, 1 eq) in EA was added HCl/EA solution. The mixture was stirred at rt for 1 h and was concentrated in vacuo. The residue was redissolved in dry DCM (150 mL) and to this solution was added AcCl (7.2 g, 47.79 mmol, 3 eq) and Et3N (13 mL, 63.72 mmol, 4 eq) at 0 °C. The mixture was stirred at 0 °C for 1 h and diluted with water. The organic layer was separated and washed with 1 N HC1 solution and brine. The mixture was dried and concentrated in vacuo. To the above crude product in MeOH (100 mL) was added K2CO3 (4.39 g, 31.86 mmol, 2 eq). The mixture was stirred at rt for 3 h and concentrated in vacuo. The residue was purified by chromatography on a silica gel column (EA/PE = 1/1, v/v) to give l-[2-(3-fluoro-4- hydroxymethyl-phenyl)-pyrrolidin-l for 3 steps) as a yellow oil.
Figure imgf000303_0004
To a solution of l-[2-(3-fluoro-4-hydroxymethyl-phenyl)-pyrrolidin-l-yl]-ethanone (1 g, 4.21 mmol, 1 eq) in EA was added HCl/EA solution. The mixture was stirred at rt for 0.5 h and the excess reagent was removed by evaporation. The residue was treated with SOCb (5 mL) and the mixture was heated under reflux for lh. The excess reagent was removed in vacuo. To the above residue and 5-(4,4,5,5-tetramethyl-[l,3,2]dioxaborolan-2- yl)-nicotinic acid methyl ester (6.31 mmol, 1.5 eq) in dioxane (40 mL) and water (10 mL) was added Na2C03 (1.7 g, 16.84 mmol, 4 eq) and Pd(dppf)Cl2 (0.17 g, 0.21 mmol, 0.05 eq). The mixture was stirred at 90 °C for 2 h under nitrogen. The mixture was allowed to cool to rt and diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/MeOH = 20/1, v/v) to give 5-[4-(l-acetyl-pyrrolidin-2-yl)-2-fluoro-benzyl]-nicotinic acid methyl ester (284 mg, 19% yields for 3 steps) as a brown oil.
Figure imgf000304_0001
5-(4-(l -Acetylpyrrolidin-2-yl)-2-fluorobenzyl^
(106 mg, 25% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-
[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 523.1, found 523.1. ¾ NMR (CDC13, 400 MHz) δ 8.81 (s, 2 H), 8.58 (s, 0.54 H), 8.47 (s, 0.46 H), 7.80 (s,
0.54 H), 7.93 (s, 0.46 H), 7.59-7.56 (m, 1 H), 7.12-7.08 (m, 1.5 H), 7.00-6.92 (m,2 H), 6.87-6.76 (m, 2.5 H),
5.12 (dd, 0.46 H), 4.85 (dd, 0.54 H), 4.76-4.72 (m, 2 H), 4.00 (s, 1 H), 3.90 (s, 1 H), 3.72-3.57 (m, 2 H), 2.45-
2.25 (m, 2 H), 2.12 (s, 1 H), 1.97-1.80 (m, 4 H), 1.81 (s, 2 H).
Example 185: Preparation of
l-(4-(l -acetylpyrrolidin-2-yl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l,6- dihydropyridine-3 -carboxamide
Figure imgf000304_0002
l-(4-(l -Acetylpyrrolidin-2-yl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l,6- dihydropyridine-3 -carboxamide (70 mg, 49%> yields for 2 steps) was prepared as described for N-(3-chloro-6- fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin- 2-yl)-pyridin-3-ylmethyl] -nicotinamide as a white solid. LRMS (M+H+) m/z calculated 539.1, found 539.1. ¾ NMR (CDCI3, 400 MHz) δ 9.12 (s, 0.6 H), 8.97 (s, 0.4 H), 8.21 (s, 0.4 H), 8.18 (s, 0.6 H), 7.73-7.66 (m, 1 H), 7.52-7.45 (m, 1.6 H), 7.06-6.67 (m, 5 H), 6.42 (d, 0.4 H), 6.36 (d, 0.6 H), 5.08 (s, 1 H), 4.97-4.83 (m, 2 H), 4.66-4.61 (m, 2 H), 3.67-3.50 (m, 2 H), 2.36-2.06 (m, 2 H), 2.09 (s, 1.2 H), 1.90-1.72 (m, 4 H), 1.77 (s, 1.8 H).
Example 186: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l -(3-fluoro-4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydro de
Figure imgf000304_0003
To a mixture of pyridin-2-ol (311 mg, 3.27 mmol, 1.05 eq) in DMF (5 mL) was added K2C03 (862 mg, 6.24 mmol, 2.0 eq) followed by methyl 4-(bromomethyl)-3-fluorobenzoate (770 mg, 3.12 mmol, 1.0 eq). The resultant mixture was stirred for 2 h, and then water (80 mL) was added. The mixture was extracted with EA and the organic phase was washed with brine, dried over Na2S04, filtered, and concentrated. The residue was purified by column chromatography on a silica gel (PE-PE/EA = 2/1, v/v) to give methyl 3-fluoro-4-((2- oxopyridin-l(2H)-yl)methyl)benzoate (650 mg, 80% ) as a white solid. LRMS (M+H+) m/z calculated 262, found 262.
Figure imgf000305_0001
The mixture of methyl 3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzoate (650 mg, 2.49 mmol, 1.0 eq) in MeOH (30 mL) was added NaBFL (1.9 g, 49.80 mmol, 20.0 eq). The resultant mixture was kept stirring at rt for overnight. The reaction mixture was concentrated. The residue was purified by chromatography on a silica gel column (DCM~DCM/MeOH = 10/1, v/v) to give l-(2-fluoro-4-(hydroxyl methyl)benzyl)pyridin-2(lH)- one (150 mg, 25% ) as a color +) m/z calculated 234, found 234.
Figure imgf000305_0002
To a mixture of 1 -(2-fluoro-4-(hydroxyl methyl)benzyl)pyridin-2(lH)-one (150 mg, 0.64 mmol, 1.0 eq) in DCM (10 mL) was added PPh3 (253 mg, 0.96 mmol, 1.5 eq) followed by NBS (125 mg, 0.70 mmol, 1.1 eq). The reaction mixture kept stirring for 2 h and the mixture was concentrated. The residue was added to the mixture of methyl 6-oxo-l, 6-dihydropyridine-3- carboxylate (98 mg, 0.64 mmol, 1.0 eq) and K2CO3 (176 mg,1.28 mmol, 2.0 eq ) in DMF (lOmL). The resultant mixture was stirred for 2 h. Water (20 mL) was added and the mixture was extracted with EA. The organic phase was dried over Na2S04, and concentrated. The residue was purified by chromatography on a silica gel column (PE-PE/EA = 10/1 to EA) to give methyl 1- (3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l, 6-dihydropyridine-3 -carboxylate (150 mg, 63%) as a white solid. LRMS (M+H+ 369.
Figure imgf000305_0003
To a mixture of methyl l-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3- carboxylate (150 mg, 0.41 mmol, 1.0 eq) in THF/water (2 mL/2mL) was added LiOH.H20 (26 mg, 0.61 mmol, 1.5 eq). The reaction mixture was kept stirring for 2 h and the mixture was acidified to pH = 6 with 1 N HC1, and concentrated to give 180 mg of crude l-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo- l,6-dihydropyridine-3-carboxylic acid, which was used directly for the next step. LRMS (M+H+) m/z calculated 355, found 355.
Figure imgf000306_0001
To a solution of l-(3-fluoro-4-((2-oxopyridin-l(2H)-yl) methyl)benzyl)-6-oxo-l,6-dihydro pyridine-3- carboxylic acid (200 mg, crude) in DMF (5 mL) was added C-(3-chloro-6-fluoro-lH-indol -5-yl)- methylamine hydrochloride (76 mg, 0.41 mmol, 1.0 eq) followed by EDCI (92 mg, 0.48 mmol, 1.2 eq), HOBT (65 mg, 0.48 mmol, 1.2 eq) and TEA (124 mg, 1.23 mmol, 3.0 eq). The reaction mixture was heated to 40 °C and kept stirring for overnight. LRMS showed the SM was consumed. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2SO i, filtered, and concentrated. The residue was purified by prep-HPLC to give N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l- (3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide (15 mg, 7%) as a white solid. LRMS (M+H+) m/z calculated 535, found 535. ¾ NMR (CD3OD, 400 MHz) δ 8.39 (d, 1 H), 7.98 (d, 1 H), 7.68 (d, 1 H), 7.51-7.55 (m, 2 H), 7.25-7.30 (m, 2 H), 7.12-7.18 (m, 3 H), 6.54-6.60 (m, 2 H), 6.38-6.42 (t, 1 H), 5.27 (s, 4 H), 4.67 (s, 2 H).
Example 187: Preparation of 5-(4-(acetamidomethyl)-5-chloro-2-fluorobenzyl)-N-((3-chloro-6-fluoro -1H- indol-5-yl)methyl)nicotinamide
Figure imgf000306_0002
The mixture of l-bromo-5-chloro-2-fluoro-4-methyl-benzene (10.0 g, 44.75 mmol, 1.0 eq), NBS (7.9 g, 44.75 mmol, 1.0 eq) and AIBN (736 mg, 4.48 mmol, 0.1 eq) in CCL (250 mL) was heated to reflux and kept stirring for 3 h under N2. The mixture was concentrated. The residue was purified by column chromatography (PE-PE/EA = 30/1, v/v) to give l -bromo-4-bromomethyl-5-chloro-2-fluoro-benzene (7.5 g, 56%) as a yellow oil.
Figure imgf000306_0003
To the mixture of l -bromo-4-bromomethyl-5-chloro-2-fluoro-benzene (7.5 g, 24.81 mmol, 1.0 eq) in DMF (50 mL) was added NaN3 (1.9 g, 29.77 mmol, 1.2 eq) at 0 °C and then the resultant mixture was warmed to rt and kept stirring for 2 h. EA (80 mL) was added. The mixture was washed with brine, and the organic phase was dried over Na2S04, filtered, and concentrated. The residue was purified by column chromatography (PE~PE/EA = 30/1, v/v) to givel-azidomethyl-4-bromo-2- chloro-5-fluoro-benzene (6 g, crude) as a yellow oil, which was used directly without further purification.
Figure imgf000307_0001
To a solution of 1 -azidomethyl-4-bromo-2- chloro-5-fluoro-benzene (4 g, 15.15 mmol, 1.0 eq) in THF (20 mL) and water (5 mL) was added PPI13 (5.9 g, 22.73 mmol, 1.5 eq) at 0 °C, and then the resultant mixture was warmed to rt and kept stirring for 4 h. The reaction mixture was concentrated and the residue was purified by column chromatography on a silica gel (DCM/MeOH = 10/1, v/v) to give 4-bromo-2-chloro -5-fluoro- benzylamine (1.8 g, 50%) as a yellow solid. +) m/z calculated 238, 240, found 238, 240.
Figure imgf000307_0002
N-(4-bromo-2- chloro-5-fluoro-benzyl)-acetamide (1.6 g, 76%) was prepared as described for 5-[4-
(acetylamino-methyl)-2-fluoro-benzyl]-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-nicotinamide as a white solid. LRMS (M+H+) m/z cal
Figure imgf000307_0003
5-[4-(Acetylamino-methyl)-5-chloro-2-fluoro-benzyl]-nicotinic acid methyl ester (550 mg, 46%>) was prepared as described for 5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3- ylmethyl] -nicotinic acid methyl ester yellow solid. LRMS (M+H+)
Figure imgf000307_0004
5-(4-(Acetamidomethyl)-5-chloro-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)nicotinamide (45 mg, 30%)) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 517, found 517. 'H NMR (DMSO-ifc, 400 MHz) δ 11.39 (br, 1 H), 9.18 (t, 1 H), 8.91 (d, 1 H), 8.64 (d, 1 H), 8.34 (t, 1 H), 8.05 (s, 1 H), 7.50-7.53 (d, 2 H), 7.45 (d, 1 H), 7.22 (d, 2 H), 7.13 (d, 1 H), 4.59 (d, 2 H), 4.25 (d, 2 H), 4.05 (s, 2 H), 1.89 (s, 3 H).
Example 188: Preparation of N-((3 -chloro-4-fluoro- 1 H-indol-5 -yl)methyl)-5 -(4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)nicotinamide
Figure imgf000307_0005
N-((3-chloro-4-fluoro-lH-indol-5-yl)m (20 mg, 13%) was prepared as described for (5-{5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin- 3-ylmethyl}-pyridin-2-ylmethyl)-carbamic acid fert-butyl ester as a white solid. LRMS (M+H+) m/z calculated 501, found 501. ¾ NMR (DMSO-ifc, 300 MHz) δ 11.57 (br, 1 H), 9.11 (t, 1 H), 8.86 (d, 1 H), 8.60 (d, 1 H), 8.04 (t, 1 H), 7.72-7.75 (m, 1 H), 7.50 (d, 1 H), 7.36-7.42 (m, 1 H), 7.11-7.25 (m, 6 H), 6.37-6.40 (m, 1 H), 6.18-6.22 (m, 1 H), 5.04 (s, 2 H), 4.56 (d, 2 H), 3.99 (s, 2 H).
Example 189: Preparation of N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)- pyridin-3 -ylmethyl] -nicotinamide
Figure imgf000308_0001
To a solution of 5-bromo-pyridine-2-carbaldehyde (2.82 g, 20 mmol, 1.0 eq) and 3-ethyl-5-(2-hydroxyethyl)- 4-methylthiazolium bromide (1.0 g, 4.0 mmol, 0.2 eq) in dry MeOH (100 mL) was added methyl acrylate (2.2 mL, 24 mmol, 1.2 eq) and Et3N (8.4 mL, 60 mmol, 3 eq). The mixture was stirred at 70 °C for 1 h. After cooling to rt, the mixture was quenched with saturated NH4CI solution. MeOH was removed by evaporation. The aqueous layer was extracted with EA. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (PE/EA = 50/1, v/v) to afford 4-(5-bromo-pyridin-2- yl)-4-oxo-butyric acid methyl ester (1.6 g 30%) as a yellow soli
Figure imgf000308_0002
To a solution of 4-(5-bromo-pyridin-2-yl)-4-oxo-butyric acid methyl ester (840 mg, 3.1 mmol, 1.0 eq) in MeOH (15 mL) was added NH4OAC (2.4 g, 31 mmol, 10 eq). The mixture was stirred at rt for 1 h, then
NaB¾CN (215 mg, 3.41 mmol, 1.1 eq) was added and the reaction mixture was stirred at refluxing for 4 h. The reaction mixture was cooled and then concentrated under reduced pressure. The residue was dissolved in EA and washed with water. The organic phase was dried and concentrated in vacuo. The residue was purified by chromatography on a silica gel column (EA) to afford 5-(5-bromo-pyridin-2-yl)-pyrrolidin-2-one (390 mg, 52%)) as a white solid.
Figure imgf000308_0003
To a solution of 5-(5-bromo-pyridin-2-yl)-pyrrolidin-2-one (390 mg, 1.63 mmol, 1 eq) and B2(Pin)2 (413 mg, 1.63 mmol, 1 eq) in dioxane (10 mL) was added KOAc (478 mg, 4.88 mmol, 3 eq) and Pd(dppf)Cl2.CH2Ci2 (66 mg, 0.08 mmol, 0.05 eq). The mixture was stirred at 95 °C for 30 h. After cooling to rt, 5-chloromethyl- nicotinic acid methyl ester (363 mg, 1.96 mmol, 1.2 eq), Pd(d f)Cl2.CH2Cl2 (66 mg, 0.08 mmol, 0.05 eq), Na2CC>3 (517 mg, 4.88 mmol, 3 eq) and water (3 mL) was added to the mixture. The mixture was stirred at 90 °C for 1 h under nitrogen atmosphere. After cooling to rt, the solvent was removed in vacuo. The residue was purified by chromatography on a silica gel column (DCM/MeOH = 20/1, v/v) to afford 5-[6-(5-Oxo- pyrrolidin-2-yl)-pyridin-3-yl %) as a yellow solid.
Figure imgf000309_0001
To a solution of 5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinic acid methyl ester (130 mg, 0.42 mmol, 1.0 eq) in THF (5 mL)/H20 (5 mL) was added L1OH.H2O (20 mg, 0.46 mmol, 1.1 eq). The mixture was stirred at 40 °C for 1 h and was acidified to pH = 5 with 1 N HC1 solution. The mixture was concentrated in vacuo and the residue was directly used without further purification. To a solution of the above crude product and C-(3-chloro-6-fluoro-lH-indol-5-yl)-methylamine (117 mg, 0.51 mmol, 1.2 eq) in DMF (8 mL) was added HATU (194 mg, 0.51 mmol, 1.2 eq) and Et3N (0.18 mL, 1.25 mmol, 3 eq). The mixture was stirred at rt for 1 h and diluted with water. The organic layer was separated and the aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/MeOH = 10/1, v/v) to give N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5- oxo-pyrrolidin-2-yl)-pyridin- 3 -ylmethyl] -nicotinamide (60 mg, 30% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 478.1, found 478.1. ¾ NMR (CD3OD, 400 MHz) δ 8.87 (d, 1 H), 8.60 (d, 1 H), 8.47 (d, 1 H), 8.10 (t, 1 H), 7.69 (dd, 1 H), 7.53 (d, 1 H), 7.35 (d, 1 H), 7.24 (s, 1 H), 7.13 (d, 1 H), 4.85- 4.81 (m, 1 H), 4.70 (s, 2 H), 4.10 (s, 2 H), 2.63-2.54 (m, 1 H), 2.45-2.39 (m, 2 H), 2.07-1.98 (m, 1 H).
Example 190: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((6-((N- cyclopropylacetamido)methyl)pyridin-3-yl) e
Figure imgf000309_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl) methyl)-5-((6-((N-cyclopropyl acetamido)methyl)pyridin-3- yl)methyl)nicotinamide (45 mg, 36%>) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5- ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3- ylmethyl] -nicotinamide as a white solid. LRMS (M+H+) m/z calculated 506, found 506. ¾ NMR (DMSO-ifc, 300 MHz) δ 11.39 (br, 1 H), 9.16 (t, 1 H), 8.90 (d, 1 H), 8.66 (d, 1 H), 8.45 (s, 1 H), 8.09 (s, 1 H), 7.60 (d, 1 H), 7.50 (d, 1 H), 7.45 (d, 1 H), 7.22 (d, 1 H), 7.09 (d, 1 H), 4.59 (d, 2 H), 4.51 (s, 1 H), 4.03 (s, 2 H), 2.77-2.81 (m, 1 H), 2.16 (s, 3 H), 1.73-1.75 (m, 4 H).
Example 191: Preparation of N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-{6-[(2-hydroxy-acetylamino)- methyl] -pyridin-3 -ylmethyl } -nicotinamide
Figure imgf000310_0001
N-(3 -chloro-6-fluoro- 1 H-indol-5-ylmethyl)-5- {6-[(2-hydroxy-acetylamino)-methyl] -pyridin-3 -ylmethyl} - nicotinamide (20 mg, 35%) was prepared as described for 5- {4-[(2-amino-acetylamino)-methyl]-2-fluoro- benzyl}-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-nicotinamide as a yellow oil. LRMS (M+H+) m/z calculated 482.1, found 482.1. ¾ NMR (CD3OD, 400 MHz) δ 8.86 (s, 1 H), 8.57 (s, 1 H), 8.39 (s, 1 H), 8.08 (d, 1 H), 7.60 (d, 1 H), 7.50 (d, 1 H), 7.28-7.22 (m, 2 H), 7.11 (d, 1 H), 4.68 (s, 2 H), 4.51 (s, 2 H), 4.05 (d, 1 H).
Example 192: Preparation of 5-((6-((2-aminoacetamido)methyl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro- 1 H-indol-5 -yl)methyl)nicotinamide
Figure imgf000310_0002
5-{6-[(2-Amino-acetylamino)-methyl]-pyridin-3-ylmethyl}-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)- nicotinamide (40 mg, 59.5%) was prepared as described for 5-(6-aminomethyl-pyridin-3-ylmethyl)-N-(3- chloro-6-fluoro-lH-indol-5-ylmethyl)-nicotinamide as yellow solid. LRMS (M+H+) m z calculated 481.1, found 481.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.49 (s, 1 H), 9.42 (t, 1 H), 9.24 (t, 1 H), 9.07 (d, 1 H), 8.87 (d, 1 H), 8.78 (d, 1 H), 8.24 (s, 1 H), 7.71 (d, 1 H), 7.52-7.48 (m, 2 H), 7.26-7.23 (m, 1 H), 4.61 (d, 4 H), 4.27 (s, 2 H), 3.71-3.66 (m, 2 H).
Example 193: Preparation of 5-((4-(acetamidomethyl)-2-oxopyridin-l(2H)-yl)methyl)-N-((3-chloro-6-fluoro- 1 H-indol-5 -yl)methyl)nicotinamide
Figure imgf000310_0003
5-((4-(Acetamidomethyl)-2-oxopyridin-l(2H)-yl)methyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)nicotinamide (73 mg, 67%> yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro- lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3- ylmethyl] -nicotinamide as a white solid. LRMS (M+H+) m/z calculated 428.1, found 428.1. ¾ NMR (CDCI3, 400 MHz) δ 9.22 (t, 1 H), 8.96 (d, 1 H), 8.69 (d, 1 H), 8.32 (t, 1 H), 8.11 (s, 1 H), 7.83 (d, 1 H), 7.51 (d, 1 H), 7.47 (d, 1 H), 7.24 (d, 1 H), 6.23 (s, 1 H), 6.17 (dd, 1 H), 5.14 (s, 2 H), 4.60 (d, 2 H), 4.08 (s, 2 H), 1.88 (s, 3 H).
Example 194: Preparation of 2-((3-(acetamidomethyl)-lH-pyrazol-l-yl)methyl)-N-((3-chloro-6-fluoro-lH- indol-5-yl)methyl)isonicotinamide
Figure imgf000311_0001
2-((3 -(Acetamidomethyl)- 1 H-pyrazol- 1 -yl)methyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5- yl)methyl)isonicotinamide (45 mg, 33% yields for 2 steps) was prepared as described for N-(3-chloro-6- fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 455.1, found 455.1. ¾ NMR (OMSO-d6, 400 MHz) δ 11.40 (s, 1 H), 9.28 (t, 1 H), 8.68 (d, 1 H), 8.15 (t, 1 H), 7.79 (d, 1 H), 7.72 (d, 1 H), 7.56 (s, 1 H), 7.51 (d, 1 H), 7.45 (d, 1 H), 7.23 (d, 1 H), 6.15 (d, 1 H), 5.42 (s, 2 H), 4.59 (d, 2 H), 4.16 (d, 2 H), 1.80 (s, 3H).
Example 197: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)isonicotinamide
Figure imgf000311_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)isonicotinamide (50 mg, 42% yields for 2 steps) was prepared as described for N-(3-chloro-6- fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin -3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 502.1, found 502.1. ¾ NMR (CD3OD, 400 MHz) δ 8.60 (d, 1 H), 8.46 (d, 1 H), 7.74-7.70 (m, 4 H), 7.56-7.53 (m, 2 H), 7.25 (s, 1 H), 7.24 (d, 1 H), 7.14 (d, 1 H), 6.55 (d, 1 H), 6.40 (dt, 1 H), 5.24 (s, 2 H), 4.71 (s, 2 H), 4.22 (s, 2 H).
Example 195, 196, 198, 199, 200 were prepared as described for N-((3-chloro-6-fluoro-lH- indol-5- yl)methyl)-2-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)isonicotinamide.
Example 201: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((N- cyclopropylacetamido)methyl)pyridi
Figure imgf000311_0003
To a solution of (5- {4-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]- pyridin-2-ylmethyl} -pyridin-2- ylmethyl)-cyclopropyl-carbamic acid tert-butyl ester (70 mg, 0.12 mmol, 1.0 eq) in EA was added HC1/EA solution. The mixture was stirred at rt for 5 h. The mixture was concentrated in vacuo and to this residue in
DCM was added AC2O (25 mg, 0.24 mmol, 2 eq) and TEA (50 mg, 0.48 mmol, 4 eq). The mixture was stirred at rt for overnight. The mixture was quenched with water. The aqueous layer was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by prep-HPLC to give N-((3-chloro- 6-fluoro-lH-indol-5-yl)methyl)-2-((6-((N-cyclopropylacetamido)methyl) pyridin-3-yl)methyl)isonicotinamide (27 mg, 43% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 506.1, found 506.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.38 (s, 1 H), 9.22 (t, 1 H), 8.63 (d, 1 H), 8.44 (s, 1 H), 7.75 (s, 1 H), 7.64-7.61 (m, 2 H), 7.51 (d, 1 H), 7.46 (d, 1 H), 7.24 (d, 1 H), 7.11 (d, 1 H), 4.59 (d, 2 H), 4.52 (s, 2 H), 4.15 (s, 2 H), 2.80- 2.77 (m, 1 H), 2.17 (s, 3 H), 0.76-0.74 (m, 4 H).
Example 202: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((N-cyclopropyl-2- hydroxyacetamido)methyl)pyridi
Figure imgf000312_0001
To a suspension of (5-{4-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]- pyridin-2-ylmethyl}-pyridin- 2-ylmethyl)-cyclopropyl-carbamic acid tert-butyl ester (100 mg, 0.18 mmol, 1.0 eq) in EA was added HCl/EA solution. The mixture was stirred at rt for 5 h. The mixture was concentrated in vacuo and residue in DMF was added hydroxy-acetic acid (16 mg, 0.27 mmol, 1.2 eq), HATU (67 mg, 0.18 mmol, 1.0 eq) and TEA (89 mg, 0.9 mmol, 5 eq). The mixture was stirred at rt for 1 h. The mixture was quenched with water extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by prep-HPLC to give N-((3-chloro-6-fluoro-lH-indol-5-yl)-methyl)-2-((6-((N-cyclopropyl-2- hydroxyacetamido)methyl)pyridin-3-yl)methyl)isonicotinamide (19 mg, 21% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 522.1, found 522.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.23 (t, 1 H), 8.63 (d, 1 H), 8.45 (s, 1 H), 7.75 (s, 1 H), 7.64-7.62 (m, 2 H), 7.50 (s, 1 H), 7.46 (d, 1 H), 7.24 (d, 1 H), 7.14 (d, 1 H), 4.59 (d, 2 H), 4.56 (s, 2 H), 4.45 (t, 1 H), 4.32 (d, 2 H), 4.15 (s, 2 H), 2.72-2.72 (m, 1 H), 0.75-0.70 (m, 4 H).
Example 203: Preparation of N-((5-chloro-lH-indazol-3-yl)methyl)-2-((6-((N- cyclopropylacetamido)methyl)pyridin-3-yl) de
Figure imgf000312_0002
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-2-((6-((N-cyclopropylacetamido)methyl)pyridin-3 - yl)methyl)isonicotinamide (12 mg, 20%>) was prepared as described for N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)-2-((6-((N-cyclopropylacetamido)methyl)pyridin-3-yl)methyl)isonicotinamide as a white solid. LRMS (M+H+) m/z calculated 489.1, found 489.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.09 (s, 1 H), 9.38 (t, 1 H), 8.63 (d, 1 H), 8.44 (s, 1 H), 7.91 (s, 1 H), 7.75 (s, 1 H), 7.62 (d, 2 H), 7.55 (d, 1 H), 7.36 (d, 1 H), 7.11 (d, 1 H), 4.80 (d, 2 H), 4.52 (s, 2 H), 4.15 (s, 2 H), 2.81-2.79 (m, 1 H), 2.18 (s, 3 H), 0.76-0.75 (m, 4 H).
Example 204: Preparation of N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-(6-pyrazol-l-ylmethyl-pyridin-3- ylmethyl)-isonicotinamide
Figure imgf000313_0001
To a solution of lH-pyrazole (1.4 g, 20.3 mmol, 1.5 eq) in THF (30 mL) was added Cs2C03 (13.2 g, 40.5 mmol, 3.0 eq) and 6-chloromethyl-nicotinic acid methyl ester (2.5 g, 13.0 mmol, 1.0 eq). The reaction mixture was stirred for 1 h at 60 °C. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated to give the crude product which was subject to silica gel chromatography (PE/EA = 3/1-1/1, v/v) to afford 6-pyrazol-l-ylmethyl-nicotinic acid methyl ester (1.9 g, 67%) as a white solid.
Figure imgf000313_0002
(6-Pyrazol-l-ylmethyl-pyridin-3-yl)-methanol (0.8 g, 50%) was prepared as described for l-(2-fluoro-4- (hydroxyl methyl)benzyl)pyridin-2(lH)-one as a white solid.
Figure imgf000313_0003
5-Chloromethyl-2-pyrazol-l-ylmethyl -pyridine (0.7 g, 81%>) was prepared as described for
chloromethyl-2-methanesulfonyl-benzy a brown solid.
Figure imgf000313_0004
2-(6-Pyrazol-l -ylmethyl-pyridin-3-ylmethyl)-isonicotinic acid methyl ester (180 mg, 40%>) was prepared as described for 2-[6-(2-oxo-2H-pyridin-l -ylmethyl)-pyridin-3-ylmethyl]-isonicotinic acid methyl ester as a yellow solid.
Figure imgf000313_0005
2-(6-Pyrazol-l -ylmethyl-pyridin-3-ylmethyl)-isonicotinic acid (170 mg, crude) was prepared as described for l-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxylic acid as a white solid, which was used without purification.
Figure imgf000314_0001
N-(3-chloro-6-fluoro-lh-indol-5-ylmethyl)-2-(6-pyrazol-l -ylm (140 mg, 50%) was prepared as described for (5- {5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin- 3-ylmethyl}-pyridin-2-ylmethyl)-carbamic acid fert-butyl ester as a white solid. LRMS (M+H+) m/z calculated 475.1, found 475.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.21 (t, 1 H), 8.61 (d, 1 H), 8.49 (s, 1 H), 7.82 (d, 1 H), 7.73 (s, 1 H), 7.62-7.66 (m, 2 H), 7.51 (d, 2 H), 7.44-7.45 (m, 2 H), 7.21 (d, 1 H), 6.91 (d,l H), 6.27 (t, 1 H), 5.38 (s, 2 H), 4.79 (s, 1 H), 4.58 (d, 2 H), 4.16 (s, 2 H).
Example 205: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000314_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide (40 mg, 27% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5- [6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 501.1, found 501.1. ¾ NMR (CD3OD, 400 MHz) δ 8.54 (dd, 1 H), 7.64 (s, 1 H), 7.64-7.58 (m, 2 H), 7.50- 7.44 (m, 2 H), 7.22-7.20 (m, 5 H), 7.10 (d, 1 H), 6.52 (d, 1 H), 6.32 (t, 1 H), 5.11 (s, 2 H), 4.66 (s, 2 H), 4.13 (s, 2 H).
Example 206, 207, 211, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, and 332 were prepared as described for N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide.
Example 208: Preparation of N-((4-aminoquinazolin-7-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000314_0003
To a solution of 7-bromo-quinazolin-4-ol (10 g, 44.4 mmol, 1.0 eq) in POCI3 (200 mL). The mixture was stirred at 120 °C for overnight. The mixture was concentrated and extracted with DCM and the combined extracts were washed with brine, dried and concentrated to give 7-bromo-4-chloro-quinazoline as a yellow solid (10 g, crude).
Figure imgf000315_0001
To a solution of 7-bromo-4-chloro-quinazoline (10 g, 41.50 mmol, 1.0 eq) in MeOH/NFL (500 mL) and was stirred at 30 °C for 48 h. The mixture was concentrated to give 7-bromo-quinazolin-4-ylamine as a yellow solid (10 g, crude).
To a solution of 7-bromo-quinazolin-4-ylamine (10 g, 45.04 mmol, 1.0 eq) in DMF (400 mL) was added Zn(CN)2 (13.22 g, 112.3 mmol, 2.5 eq) and Pd(pph3)4 (5.2 g, 4.51 mmol, 0.1 eq). The mixture was stirred at 120 °C for 4 h. The mixture was concentrated and extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/MeOH = 50/1, v/v) to give 4-amino-quinazoline-7-carb a yellow oil.
Figure imgf000315_0003
To a solution of 4-amino-quinazoline-7-carbonitrile (4.5 g, 26.47 mmol, 1 eq) in MeOH (200 mL), DMF (200 mL) and ammonium hydroxide (100 mL) was added Raney Ni (4.0 g). The mixture was stirred at 40 °C overnight under hydrogen atmosphere. Raney Ni was filtered off and the filtrate was concentrated in vacuo to give 7-aminomethyl-quinazolin-4-y
Figure imgf000315_0004
N-((4-aminoquinazolin-7-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide (15 mg, 13% yield for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as an off-white solid. LRMS (M+H+) m/z calculated 577.1, found 577.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.42 (t, 1 H), 8.63-8.64 (d, 1 H), 8.36 (s, 1 H), 8.15-8.17 (d, 1 H), 7.64-7.76 (m, 5 H), 7.54 (s, 1 H), 7.41-7.43 (m, 2 H), 7.20-7.27 (m, 4 H), 6.38-6.40 (d, 1 H), 6.21 (t, 1 H), 5.04 (s, 2 H), 4.61-4.63 (d, 2 H), 4.13 (s, 2 H).
Example 209: Preparation of N-((8-aminoquinolin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000315_0005
To a solution of 2-[4-(2-oxo-2H-pyridin-l-ylmethyl)-benzyl]-isonicotinic acid methyl ester (162 mg, 0.49 mmol, 1.0 eq) in THF (3 mL)/H20 (2 mL) was added L1OH.H2O (25 mg, 0.58 mmol, 1.2 eq). The mixture was stirred at 40 °C for 1 h and was acidified to pH = 5 with 1 N HC1 solution. The mixture was concentrated in vacuo and the residue was directly used without further purification. To a solution of the above crude product and 3-aminomethyl-quinolin-8-ylamine (102 mg, 0.49 mmol, 1.0 eq) in DMF (5 mL) was added
HOBT (99 mg, 0.73 mmol, 1.5 eq), EDCI (140 mg, 0.73 mmol, 1.5 eq) and Et3N (148 mg, 1.46 mmol, 3 eq). The mixture was stirred at rt for 12 h and concentrated. The residue was purified by prep-HPLC to give N-((8- aminoquinolin-3 -yl)methyl)-2-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)isonicotinamide (15 mg, 6.5% yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 476.2, found 476.2. ¾ NMR (DMSO-<¾, 300 MHz) δ 9.39 (t, 1 H), 8.71 (d, 1 H), 8.63 (d, 1 H), 8.03 (s, 1 H), 7.75 (d, 1 H), 7.70 (s, 1 H), 7.63 (d, 1 H), 7.42 (t, 1 H), 7.36-7.19 (m, 5 H), 7.04 (d, 1 H), 6.84 (s, 1 H), 6.40 (s, 1 H), 6.20 (t, 1 H), 5.04 (s, 2 H), 4.65 (d, 2 H), 4.12 (s, 2 H).
Example 210: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000316_0001
N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide (25 mg, 18% yields for 2 steps) was prepared as described for N-((8-aminoquinolin-3-yl)methyl)-2-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)isonicotinamide as an off- white solid. LRMS (M+H+) m/z calculated 476.2, found 476.2. 'H NMR (CD3OD, 400 MHz) δ 8.59 (d, 1 H), 8.09 (d, 1 H), 7.73-7.62 (m, 5 H), 7.48 (d, 1 H), 7.46 (d, 1 H), 7.23 (s, 4 H), 6.94 (d, 1 H), 6.55 (d, 1 H), 6.36 (d, 1 H), 5.13 (s, 2 H), 4.70 (s, 2 H), 4.17 (s, 2 H).
Example 212: Preparation of N-((5-chloro-lH-pyrrolo[2,3-b]pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000316_0002
To a suspension of 5-chloro-lH-pyrrolo[2,3-b]pyridine (10.0 g, 65.5 mmol, 1 eq) in AcOH (56.7 mL) and water (28.3 mL) was added hexamethylenetetramine (11.9 g, 85.2 mmol, 1.3 eq). The mixture was stirred under reflux overnight, followed by addition of 200 mL of water. After stirring for 30 min, the reaction mixture was filtered to recover the solid, then dried under air to give 5-cloro-lH-pyrrolo[2,3-b]pyridine-3- carbaldehyde (6.2 g, 53%>) as a yellow solid.
Figure imgf000316_0003
To a solution of 5-cloro-lH-pyrrolo[2,3-b]pyridine-3-carbaldehyde (1.0 g, 5.55 mmol, 1 eq) in EtOH (40 mL) and water (10 mL) was added hydroxylammonium chloride (575 mg, 8.33 mmol, 1.5 eq) and Na2C03 (1.06 g, 10.0 mmol, 1.8 eq). The mixture was stirred under reflux for 3 h and then cooled to rt. The precipitate was collected by filtration to give 5-chloro-lH-pyrrolo[2,3-b]pyridine-3-carbaldehyde oxime (1.0 g, 92% ) as an off white solid.
Figure imgf000317_0001
To a solution of 5-chloro-lH-pyrrolo[2,3-b]pyridine-3-carbaldehyde oxime (200 mg, 2 mmol, 1 eq) in MeOH (5 mL) was added NiCb (128 mg, 1 mmol, 1 eq) and NaB¾ (228 mg, 6 mmol, 6 eq). The mixture was stirred at rt for 5 h under hydrogen atmosphere. NiCb was filtered off and the filtrate was concentrated in vacuo to give C-(5-chloro-lH-pyrrolo[2,3- as a yellow solid.
Figure imgf000317_0002
N-((5-chloro-lH-pyrrolo[2,3-b]pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide (49 mg, 34%> yields for 2 steps) was prepared as described for N-(3-chloro- 6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 484.2, found 484.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.76 (s, 1 H), 9.13 (t, 1 H), 8.59 (d, 1 H), 8.18 (dd, 1 H), 7.75 (d, 1 H), 7.66 (s, 1 H), 7.57 (d, 1 H), 7.52 (d, 1 H), 7.42-7.36 (m, 1 H), 7.25-7.18 (m, 4 H), 6.40 (d, 1 H), 6.20 (t, 1 H), 5.03 (s, 2 H), 4.57 (s, 2 H), 4.09 (s, 2 H).
Example 223: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3-methyl-lH-pyrazol-l - yl)methyl)benzyl)isonicotinamide
Figure imgf000317_0003
To a solution of 2-(4-chloromethyl-benzyl)-isonicotinic acid methyl ester (100 mg, 0.36 mmol, 1 eq) and 3- methyl-lH-pyrazole (90.72 mg, 1.8 mmol, 3 eq) in THF (5 mL) /DMF (2 mL) was added KO'Bu (79.92 mg, 0.72 mmol, 2 eq). The mixture was stirred at rt for overnight. The mixture was concentrated in vacuo and the residue was directly used without further purification. To a solution of the above crude product and C-(3- chloro-6-fluoro-lH-indol-5-yl)-methylamine (102 mg, 0.43 mmol, 1.2 eq) in DMF (5 mL) was added HATU (136.8 mg, 0.36 mmol, 1.0 eq) and Et3N (0.19 mg, 1.44 mmol, 4 eq). The mixture was stirred at rt for overnight and concentrated. The residue was purified by prep-HPLC to give N-((3-chloro-6-fluoro-lH-indol- 5-yl)methyl)-2-(4-((3 -methyl- lH-pyrazol-l-yl)methyl)benzyl)isonicotinamide (10 mg, 6%> yields for 2 steps) as a white solid. LRMS (M+H+) m/z calculated 488.1, found 488.1. ¾ NMR (DMSO-tfc, 400 MHz) δ 11.39 (s, 1 H), 9.21 (t, 1 H), 8.61-8.60 (d, 1 H), 7.69 (s, 1 H), 7.63-7.61 (m, 2 H), 7.50 (s, 1 H), 7.45-7.43 (d, 1 H), 7.24-7.21 (m, 3 H), 7.13-7.11 (d, 1 H), 7.02-7.00 (d, 1 H), 6.03-5.99 (m, 1 H), 5.22-5.15 (d, 2 H), 4.10 (s, 2 H), 4.60-4.58 (d, 2 H), 4.58-4.57 (d, 2 H), 4.11-4.08 (m, 2 H), 2.17-2.11 (d, 2 H).
Example 224: Preparation of N-(3-chloro-6-fluoro-lh-indol-5-ylmethyl)-2-(4-pyrazol-l -ylmethyl-benzyl)- isonicotinamide
Figure imgf000318_0001
To a solution of lH-pyrazole (0.52 g, 7.58 mmol, 1.0 eq) in THF (30 mL) was added NaH (0.18 g, 7.58 mmol, 1.0 eq) and 1 ,4-bis-bromomethyl-benzene (3.0 g,l 1.36 mmol, 1.5 eq). The reaction mixture was stirred at rt for 1 h. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated to give the crude product which was subject to silica gel chromatography (PE/EA = 3/1-1/1, v/v) to afford 1 -(4-bromomethyl -benzyl)- lH-pyrazole (1.5 g, 79%) as a white solid.
Figure imgf000318_0002
2-(4-Pyrazol-l -ylmethyl-benzyl)-isonicotinic acid methyl ester (130 mg, 21%) was prepared as described for 2-[6-(2-oxo-2H-pyridin-l -ylmethyl) methyl ester as a white solid.
Figure imgf000318_0003
N-(3-chloro-6-fluoro-lh-indol-5-ylmethyl)-2-(4-pyrazol-l -ylmethyl-benzyl)-isonicotinamide (30 mg, 15.1%>) was prepared as described for N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l -(3-fluoro-4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide as a white solid. LRMS (M+H+) m/z calculated 474.1, found 474.1. ¾ NMR (MeOD, 400 MHz) δ 8.59 (d, 1 H), 7.63-7.68 (m, 3 H), 7.50-7.54 (m, 2 H), 7.28 (s, 1 H), 7.26 (s, 2 H), 7.13-7.18 (m, 3 H), 6.32 (t, 1 H), 5.32 (s, 2 H), 5.15 (s, 1 H), 4.71 (s, 2 H), 4.20 (s, 2 H).
Example 225: Preparation of 2-(4-((lH-imidazol-l -yl)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide
Figure imgf000319_0001
2-(4-((lH-Imidazol-l-yl)methyl)benzyl)-N (20 mg,
12% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5- oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl] -nicotinamide as a off-white solid. LRMS (M+H+) m/z calculated 474.1, found 474.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.23-9.20 (t, 1 H), 8.61-8.60 (d, 1 H), 7.95-7.91 (m, 1 H), 7.69 (s, 1 H), 7.63-7.61 (d, 1 H), 7.51-7.50 (d, 1 H), 7.45-7.44 (d, 1 H), 7.28-7.17 (m, 6 H), 6.89 (s, 1 H), 5.16 (s, 2 H), 4.58-4.57 (d, 2 H), 4.12 (s, 2 H).
Example 226: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((5-oxo-lH-l,2,4-triazol- 4(5H)-yl)methyl)benzyl)isonicotinamide
Figure imgf000319_0002
N-((3 -chloro-6-fluoro- 1 H-indol-5-yl)methyl)-2-(4-((5 -oxo- 1 H- 1 ,2,4-triazol-4(5H)- yl)methyl)benzyl)isonicotinamide (17 mg, 11% yields for 2 steps) was prepared as described for N-(3-chloro- 6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 491.1, found 491.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.67 (s, 1 H), 11.39 (s, 1 H), 9.21 (t, 1 H), 8.62-8.60 (d, 1 H), 7.91 (s, 1 H), 7.70 (s, 1 H), 7.62-7.61 (d, 1 H), 7.51-7.50 (d, 1 H), 7.46-7.44 (d, 1 H), 7.28-7.18 (m, 5 H), 4.67 (s, 2 H), 4.59-4.57 (d, 2 H), 4.12 (s, 2 H).
Example 227: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3,5-dimethyl-lH-pyrazol-l - yl)methyl)benzyl)isonicotinamide
Figure imgf000319_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3,5-dimethyl-lH-pyrazol-l - yl)methyl)benzyl)isonicotinamide (8 mg, 5%> yields for 2 steps) was prepared as described for N-((3-chloro-6- fluoro-lH-indol-5-yl)methyl)-2-(4-((3-methyl-lH-pyrazol-l -yl)methyl)benzyl)isonicotinamide as a white solid. LRMS (M+H+) m/z calculated 502.1, found 502.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.40 (s, 1 H), 9.22 (t, 1 H), 8.62-8.61 (d, 1 H), 7.69 (s, 1 H), 7.63-7.62 (d, 1 H), 7.52-7.51 (d, 1 H), 7.46-7.44 (d, 1 H), 7.25- 7.22 (m, 3 H), 7.03-7.01 (d, 2 H), 5.82 (s, 1 H), 5.12 (s, 2 H), 4.59-4.58 (d, 2 H), 4.12 (s, 2 H), 2.14 (s, 3 H), 2.08 (s, 3 H).
Example 228: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((4-methyl-lH-pyrazol-l - yl)methyl)benzyl)isonicotinamide
Figure imgf000320_0001
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((4-methyl-lH-pyrazol-l - yl)methyl)benzyl)isonicotinamide (66 mg, 37% yields for 2 steps) was prepared as described for N-((3-chloro- 6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3-methyl-lH-pyrazol-l -yl)methyl)benzyl)isonicotinamide as a white solid. LRMS (M+H+) m/z calculated 588.1 , found 588.1. ¾ NMR (OMSO-d6, 300 MHz) δ 1 1.43 (d, 1 H), 9.35 (d, 1 H), 8.72-8.71 (d, 1 H), 7.85-7.70 (m, 2 H), 7.51 -7.45 (m, 3 H), 7.27-7.13 (m, 6 H), 5.50-5.48 (d, 2 H), 4.60 (d, 2 H), 4.20 (s, 2 H), 1.97 (s, 3 H).
Example 229: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3-fluoro-2-oxopyridin- l (2H)-yl)methyl)benzyl)isonicotinamide
Figure imgf000320_0002
N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-(4-hydroxymethyl-benzyl)-isonicotinamide (6 g, 91%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3- ylmethyl] -nicotinamide as a yellow solid.
Figure imgf000320_0003
To a solution of N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-(4-hydroxymethyl-benzyl)-isonicotinamide (4 g, 9.4 mmol, 1 eq) in DCM (100 mL) was added TEA (3.2 mL, 23.5 mmol, 2.5.0 eq) and MsCl (1.2 g, 1 1.28 mmol, 1.2 eq). The mixture was stirred at rt for overnight and diluted with water. The mixture was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by chromatography on a silica gel column (DCM/MeOH = 100/1 , v/v) to give N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-(4- chloromethyl-benzyl)-isonicotinami
Figure imgf000320_0004
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3-fluoro-2-oxopyridin-l (2H)- yl)methyl)benzyl)isonicotinamide (25 mg, 21 >) was prepared as described for 3-fluoro-4-((2-oxopyridin- l (2H)-yl)methyl)benzoate as an off-white solid. LRMS (M+H+) m/z calculated 519.1 , found 519.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 1 1.41 (s, 1 H), 9.25-9.23 (t, 1 H), 8.63-8.62 (d, 1 H), 7.72 (s, 1 H), 7.65-7.63 (m, 2 H), 7.52-7.46 (m, 2 H), 7.40-7.35 (m, 1 H), '.29-7 '.22 (m, 5 H), 6.23-6.18 (m, 1 H), 5.13 (s, 2 H), 4.61-4.60 (d, 2 H), 4.13 (s, 2 H).
Example 232, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244 were prepared as N-((3-chloro-6-fluoro- lH-indol-5-yl)methyl)-2-(4-((3-fluoro-2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide.
Example 230: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-fluoro-2-oxopyridin- l(2H)-yl)methyl)benzyl)isonicotinamide
Figure imgf000321_0001
To a solution of 2-(4-chloromethyl-benzyl)-isonicotinic acid methyl ester (1.1 g, 3.99 mmol, 1.0 eq) and 5- fluoro-pyridin-2-ol (0.68 g, 5.99 mmol, 1.5 eq) in DMF (35 mL) was added K2C03 (1.1 g, 7.98 mmol, 2 eq). The mixture was stirred at rt overnight, then concentrated in vacuum and the residue was diluted with EA and washed with water. The organic layer was dried over Na2S04, filtered and concentrated. The residue was purified by silica gel column chromatography (PE/EA= 1/1, v/v) to get N-((6-amino-2,4-dimethylpyridin-3- yl)methyl)-2-(4-((5-fluoro-2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide (1.02 g, 72.8%) as an off white solid.
Figure imgf000321_0002
To a solution of 2-[4-(5-fluoro-2-oxo-2H-pyridin-l -ylmethyl)-benzyl]-isonicotinic acid methyl ester (100 mg, 0.284 mmol, 1.0 eq) in THF (10 mL)/H20 (2 mL) was added LiOH H20 (14.3 mg, 0.341 mmol, 1.2 eq). The mixture was stirred at 40 °C for 1 h and was acidified to pH 5-6 with 1 N HC1 solution. The mixture was concentrated in vacuum and the residue was directly used without further purification. To a solution of the crude product and 5-aminomethyl-4,6-dimethyl-pyridin-2-ylamine (63.6 mg, 0.284 mmol, 1.0 eq) in DMF (8 mL) were added HATU (162 mg, 0.426 mmol, 1.5 eq) and Et3N (115 mg, 1.136 mmol, 4 eq). The mixture was stirred at rt for 4 h then concentrated and the residue was purified by prep-HPLC to give N-(6-amino-2,4- dimethyl-pyridin-3-ylmethyl)-2-[4-(5-fluoro-2-oxo-2H-pyridin-l -ylmethyl)-benzyl]-isonicotinamide (45 mg,
33.6%) as an off white solid. LCMS (M+H+) m/z calculated 472.2, found 472.2. ¾ NMR (DMSO-tfc, 400 MHz) δ 8.60 (t, 1 H), 8.56 (d, 1 H), 8.00-8.02 (m, 1 H), 7.66 (s, 1 H), 7.53-7.58 (m, 2 H), 7.24 (t, 4 H), 6.42- 6.45 (m, 1 H), 6.13 (s, 1 H), 5.68 (s, 2 H), 4.99 (s, 2 H), 4.34 (d, 2 H), 4.10 (s, 2 H), 2.30 (s, 3 H), 2.17 (s, 3 H). Example 231: Preparation of N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((5-fluoro-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000322_0001
To a solution of 2-[4-(5-fluoro-2-oxo-2H-pyridin-l -ylmethyl)-benzyl]-isonicotinic acid methyl ester (100 mg, 0.284 mmol, 1.0 eq) in THF (10 mL)/ H20 (2 mL) was added LiOH H20 (14.3 mg, 0.341 mmol, 1.2 eq). The mixture was stirred at 40 °C for 1 h and was acidified to pH 5-6 with 1 N HC1 solution. The mixture was concentrated in vacuum and the residue was directly used without further purification. To a solution of the crude product (95.6 mg, 0.284 mmol, 1.0 eq) and 5-aminomethyl-6-methyl-pyridin-2-ylamine (59 mg, 0.284 mmol, 1.0 eq) in DMF (8 mL) were added HATU (162 mg, 0.426 mmol, 1.5 eq) and Et3N (115 mg, 1.136 mmol, 4 eq). The mixture was stirred at rt for 4 h then concentrated and the residue was purified by prep- HPLC to give N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((5-fluoro-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide (65 mg, 50%) as an off-white solid. LCMS (M+H+) m/z calculated 458.2, found 458.2. Ή NMR (DMSO-ifc, 400 MHz) δ 8.95 (t, 1 H), 8.58 (d, 1 H), 8.01 (d, 1 H), 7.67 (s, 1 H), 7.55- 7.59 (m, 2 H), 7.24 (t, 5 H), 6.41-6.45 (m, 1 H), 6.23 (d, 1 H), 5.72 (s, 2 H), 4.99 (s, 2 H), 4.28 (d, 2 H), 4.10 (s, 2 H), 2.27 (s, 3 H).
Example 233: Preparation ofN-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-(4-hydroxymethyl-benzyl)- isonicotinamide
Figure imgf000322_0002
To a solution of compound N-[(3-chloro-6-fluoroindol-5-yl)methyl] {2-[(4-{[(4- methoxyphenyl)methoxy]methyl}phenyl)methyl](4-pyridyl)}carboxamide (0.62 g, 1.14 mmol, 1 eq) in
DCM/H20 (18 mL/1 mL) was added DDQ (0.41g, 1.82 mmol, 1.6 eq). The mixture was stirred at rt for 0.5 h.
The mixture was diluted with EA and water. The organic layer was separated and the aqueous layer was extracted with EA. The combined organic layers were dried and concentrated. The residue was purified by chromatography on a silica gel column (PE/EA = 1/1, v/v) to give N-[(3-chloro-6-fluoroindol-5-yl)methyl](2- {[4-(hydroxymethyl)phenyl]methyl}(4-pyridyl))carboxamide (12 mg, 25%) as a white solid. LRMS (M+H+) m z calculated 424.2, found 424.1.
¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.20-9.23 (t, 1 H), 8.62-8.63 (d, 1 H), 7.70 (s, 1 H), 7.62- 7.63 (d, 1 H), 7.44-7.50 (m, 2 H), 7.21-7.24 (m, 5H), 5.07-5.10 (m, 1 H), 4.57-4.59 (d, 2 H), 4.42-4.44 (d, 2 H), 4.14 (s, 2 H).
Example 245: Preparation of N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000323_0001
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide (110 mg, 28.3%) was prepared as described for N-((6-amino-2- methylpyridin-3 -yl)methyl)-2-(4-((5-fluoro-2-oxopyridin- 1 (2H)-yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m z calculated 465.2, found 465.2. ¾ NMR (CD3OD, 400 MHz) δ 8.58 (d, 1 H), 8.48 (d, 1 H), 7.60-
7.66 (m, 3 H), 7.41 (d, 1 H), 7.31 (dd, 4 H), 6.59 (d, 1 H), 6.42 (d, 1 H), 5.17 (s, 2 H), 4.44 (s, 2 H), 4.20 (s, 2 H).
Example 246: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-cyano-2-oxopyridin- l(2H)-yl)methyl)benzyl)isonicotinamide
Figure imgf000323_0002
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide (270 mg, 40.5%>) was prepared as described for N-(6-amino-2,4-dimethyl- pyridin-3-ylmethyl)-2-[4-(5-fluoro-2-oxo-2H-pyridin-l -ylmethyl) -benzyl] -isonicotinamide as an off-white solid. LRMS (M+H+) m/z calculated 478.9, found 478.9. Ή NMR ( DMSO-ίΛ,, 300 MHz) δ 8.77 (d, 1 H), 8.54-8.61 (m, 2 H), 7.65-7.69 (m, 2 H), 7.55-7.57 (in, 1 H), 7.25 (s, 4 H), 6.49 (d, 1 H), 6.11 (s, 1 H), 5.67 (s, 2 H), 5.04 (d, 2 H), 4.33 (d, 2 H), 4.10 (d, 2 H), 2.29 (s, 3 H), 2.15 (s, 3 H).
Example 247: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000323_0003
Figure imgf000324_0001
To a solution of 2-[4-(5-cyano-2-oxo-2H-pyridin-l -ylmethyl)-benzyl]-isonicotinic acid methyl ester (100 mg, 0.278 mmol, 1.0 eq) in THF (10 mL)/H20 (2 mL) was added LiOH H20 (15 mg, 0.334 mmol, 1.2 eq). The mixture was stirred at 40 °C for 2 h and was acidified to pH 5-6 with 1 N HCl solution. The mixture was concentrated in vacuum and the residue was directly used without further purification. To a solution of the crude product and 5-aminomethyl-4,6-dimethyl-pyridin-2-ylamine (62.2 mg, 0.278 mmol, 1.0 eq) in DMF (8 mL) were added HOBT (56.4 mg, 0.418 mmol, 1.5 eq), EDCI (80 mg, 0.418 mmol, 1.5 eq) and Et3N (1 13 mg, 1.1 14 mmol, 4 eq). The mixture was stirred at 40 °C for 4 h, then concentrated and the residue was purified by prep-HPLC to give N-((l -aminoisoquinolin-6-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l (2H)- yl)methyl)benzyl)isonicotinamide (17 mg, 12.2%) as a yellow solid. LRMS (M+H+) m/z calculated 500.9, found 500.9. ¾ NMR. (CD3OD, 400 MHz) δ 8.46 (d, 1 H), 8.30 (d, 1 H), 7.94 (d, 1 H), 7.58-7.60 (m, 2 H), 7.52 (dd, 1 H), 7.49 (s, 1 H), 7.45 (dd, 1 H), 7.35 (dd, 1 H), 7.15 (dd, 4 H), 6.81 (d, 1 H), 6.41 (d, 1 H), 5.00 (s, 2 H), 4.58 (s, 2 H), 4.06 (s, 2 H), 3.20-3.21 (m, 2 H).
Example 248: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((3-cyano-2-oxopyridin- 1 (2H)-yl)methyl)benzyl)isonicotinamide
Figure imgf000324_0002
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((3-cyano-2-oxopyridin-l (2H)- yl)methyl)benzyl)isonicotinamide (15 mg, 1 1.2%) was prepared as described for N-((6-amino-2,4- dimethylpyridin-3-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l (2H)-yl)methyl)benzyl)isonicotinamide. LRMS
(M+H+) m/z calculated 478.9, found 478.9. ! i NMR (CD3OD, 400 MHz) δ 8.42 (d, 1 H), 7.91 (d, 2 H), 7.50 (s, 1 H), 7.44 (dd, 1 H), 7.14 (dd, 4 H), 6.33 (t, 1 H), 6.19 (s, 1 H), 5.05 (s, 2 H), 4.38 (s, 2 H), 4.04 (s, 2 H), 2.27 (s, 3 H), 2.14 (s, 3 H).
Example 249: Preparation of N-((l -aminoisoquinolin-6-yl)methyl)-2-(4-((3-cyano-2-oxopyridin-l (2H)- yl)methyl)benzyl)isonicotinamide
Figure imgf000324_0003
N-((l -aminoisoquinolin-6-yl)methyl)-2-(4-((3-cyano-2-oxopyridin-l (2H)-yl)methyl)benzyl)isonicotinamide (30 mg, 21.5%)) was prepared as described for N-((l -aminoisoquinolin-6-yl)methyl)-2-(4-((5-cyano-2- oxopyridin-l (2H)-yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 500.8, found 500.8. ¾ NMR (DMSO-ί/ή, 300 MHz) δ 9.36-9.39 (m, 1 H), 8.63 (d, 1 H), 8.13-8.23 (in, 3 H), 7.54-7.77 (m, 4 H), 7.27- 7.41 (in, 5 H), 6.85-6.87 (m, 1 H), 6.75 (d, 1 H), 6.42-6.45 (m, 1 H), 5.12 (d, 2 H), 4.60 (t, 1 H), 4.14 (d, 2 H). Example 250: Preparation of methyl N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyridin-3- ylmethyl)benzyl)isonicotinamide
Figure imgf000325_0001
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyridin-3-ylmethyl)benzyl)isonicotinamide (17 mg, 16%) was prepared as described for 2-(3-fluoro-4-formyl-phenyl)-pyrrole-l -carboxylic acid fert-butyl ester as a white solid. LRMS (M+H+) m/z calculated 485.1 , found 485.1. ¾ NMR (OMSO-d6, 400 MHz) δ 1 1.41 (s, 1 H), 9.25-9.22 (t, 1 H), 8.63-8.61 (d, 1 H), 8.49 (s, 1 H), 8.40-8.39 (d, 1 H), 7.70-7.45 (m, 5 H), 7.30-7.16 (m, 6 H), 4.60-4.59 (d, 1 H), 4.10 (s, 2 H), 3.92 (s, 2 H).
Example 251: Preparation of methyl N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyrimidin-5- ylmethyl)benzyl)isonicotinamide
Figure imgf000325_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyrimidin-5-ylmethyl)benzyl)isonicotinamide (12 mg, 1 1%) was prepared as described for 2-(3-fluoro-4-formyl-phenyl)-pyrrole-l -carboxylic acid tert-butyl ester as a white solid. LRMS (M+H+) m/z calculated 485.1 , found 485.1. ¾ NMR (DMSO-tfc, 400 MHz) δ 1 1.41 (s, 1 H), 9.24-9.22 (t, 1 H), 9.03 (s, 1 H), 8.70-8.60 (m, 3 H), 7.70 (s, 1 H), 7.64-7.62 (m, 1 H), 7.52-7.45 (m, 2 H), 7.25-7.19 (m, 5 H), 4.60-4.58 (d, 2 H), 4.1 1 (s, 2 H), 3.94 (s, 2 H).
Example 252: Preparation of methyl N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(furan-2- ylmethyl)benzyl)isonicotinamide
Figure imgf000325_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(furan-2-ylmethyl)benzyl)isonicotinamide (1 1 mg, 1 1%) was prepared as described for 2-(3-fluoro-4-formyl-phenyl)-pyrrole-l -carboxylic acid tert-butyl ester as a white solid. LRMS (M+H+) m/z calculated 474.1 , found 474.1. ¾ NMR (DMSO-tfc, 400 MHz) δ 1 1.41 (s, 1 H), 9.24 (t, 1 H), 8.63-8.62 (d, 1 H), 7.70 (s, 1 H), 7.64-7.62 (d, 1 H), 7.52-7.44 (m, 3 H), 7.25-7.21 (m, 3 H), 7.15-7.13 (m, 2 H), 6.34 (s, 1 H), 6.1 1 -6.10 (d, 1 H), 4.60-4.58 (d, 2 H), 4.1 1 (s, 2 H), 3.91 (s, 2 H).
Example 253: Preparation of methyl 2-(4-((lH-pyrazol-3-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol- 5-yl)methyl)isonicotinamide
Figure imgf000326_0001
2-(4-(( 1 H-pyrazol-3 -yl)methyl)benzyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5 -yl)methyl)isonicotinamide (13 mg, 13%) was prepared as described for 2-(3-fluoro-4-formyl-phenyl)-pyrrole-l -carboxylic acid fert-butyl ester as a white solid. LRMS (M+H+) m/z calculated 474.1, found 474.1. ¾ NMR (DMSO-tfc, 400 MHz) δ 11.44 (s, 1 H), 9.25 (t, 1 H), 8.63-8.61 (d, 1 H), 7.70-7.63 (m, 2 H), 7.51-7.44 (m, 3 H), 7.25-7.14 (m, 5 H), 7.06 (s, 1 H), 5.99 (s, 1 H), 4.59-4.58 (d, 2 H), 4.10 (s, 2 H), 3.87 (s, 2 H).
Example 254: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((l-methyl-lH-pyrazol-5- yl)methyl)benzyl)isonicotinamide
Figure imgf000326_0002
N-((3 -chloro-6-fluoro- 1 H-indol-5 -yl)methyl)-2-(4-(( 1 -methyl- 1 H-pyrazol-5 - yl)methyl)benzyl)isonicotinamide (10 mg, 9%) was prepared as described for 2-(3-fluoro-4-formyl-phenyl)- pyrrole- 1 -carboxylic acid tert-butyl ester as an off-white solid. LRMS (M+H+) m/z calculated 488.2, found 488.2. 'H NMR (CD3OD, 400 MHz) δ 8.53 (d, 1 H), 7.62 (s, 1 H), 7.59 (d, 1 H), 7.46 (d, 1 H), 7.28 (d, 1 H), 7.18-7.16 (m, 3 H), 7.09-7.05 (m, 3 H), 5.97 (d, 1 H), 4.64 (s, 2 H), 4.12 (s, 2 H), 4.95 (s, 2 H), 3.61 (s, 3 H). Example 255: Preparation of 2-(4-(acetamidomethyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide
Figure imgf000326_0003
2-(4-(Acetamidomethyl)benzyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5-yl)methyl)isonicotinamide (45 mg, 29%> yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl] -nicotinamide. LRMS (M+H+) m/z calculated 466.1, found 466.1. ¾ NMR (CD3OD, 400 MHz) δ 8.59 (d, 1 H), 7.68 (s, 1 H), 7.63 (dd, 1 H), 7.52 (d, 1 H), 7.27-7.22 (m, 5 H), 7.15 (d, 1 H), 4.71 (s, 2 H), 4.32 (s, 2 H), 4.20 (s, 2 H), 1.98 (s, 3 H).
Example 256: Preparation of 2-(4-(acetamidomethyl)benzyl)-N-((3-chloro-4-fluoro-lH-indol-5-yl) methyl)isonicotinamide
Figure imgf000326_0004
2-(4-(Acetamidomethyl)benzyl)-N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)isonicotinamide (30 mg, 20%) was prepared as described for (5- {5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-3- ylmethyl}-pyridin-2-ylmethyl)-carbamic acid fert-butyl ester as a white solid. LRMS (M+H+) m/z calculated 465, found 465. ¾ NMR (DMSO-ifc, 300 MHz) δ 11.57 (br, 1 H), 9.19 (t, 1 H), 8.60 (d, 1 H), 8.24 (br, 1 H), 7.66 (s, 1 H), 7.60 (d, 1 H), 7.50 (d, 1 H), 7.10-7.23 (m, 6 H), 4.56 (s, 2 H), 4.18 (d, 2 H), 4.10 (s, 2 H), 1.83 (s, 3 H).
Example 257: Preparation of 2-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro- 6-fluoro- 1 H-indol-5 -yl)methyl)isonicotinamide
Figure imgf000327_0001
2-(3-Chloro-4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide (15 mg, 12%) was prepared as described for N-(3-chloro-6-fluoro-l H-indol-5 - ylmethyl)-2-(6-pyrazol-l-ylmethyl-pyridin-3-ylmethyl)-isonicotinamide as a white solid. LRMS (M+H+) m z calculated 535, found 535. ¾ NMR (CD3OD, 400 MHz) δ 8.62 (d, 1 H), 7.77 (s, 1 H), 7.73 (d, 1 H), 7.50- 7.59 (m, 3 H), 7.40 (s, 1 H), 7.23 (s, 1 H), 7.19 (d, 1 H), 7.12 (d, 1 H), 7.01 (d, 1 H), 6.56 (d, 1 H), 6.38 (t, 1 H), 5.24 (s, 2 H), 4.69 (d, 2 H), 4.21 (s, 2 H).
Example 258: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-fluoro-4-((2-oxopyridin - l(2H)-yl)methyl)benzyl)isonicotinamide
Figure imgf000327_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-fluoro-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide (83 mg, 59%>) was prepared as described for 2-(3-chloro-4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5-yl)methyl)isonicotinamide. LRMS (M+H+) m/z calculated 519, found 519. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.22 (t, 1 H), 8.63 (d, 1 H), 7.74 (d, 1 H), 7.69 (d, 1 H), 7.65 (d, 1 H), 7.50 (d, 1 H), 7.40-7.46 (m, 2 H), 7.22 (d, 1 H), 7.15 (d, 1 H), 7.04-7.10 (m, 2 H), 6.38 (d, 1 H), 6.23 (t, 1 H), 5.07 (s, 2 H), 4.58 (d, 2 H), 4.15 (s, 2 H).
Example 259: Preparation of N-(3-Chloro-6-fluoro-lH-indol-5-ylmethyl)-2-[3-cyano-4-(2-oxo-2H-pyridin-l- ylmethyl)-benzyl]-isonicotinamide
Figure imgf000327_0003
N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-[3-cyano-4-(2-oxo-2H-pyridin-l -ylmethyl)-benzyl]- isonicotinamide (0.17 mg, 25.8%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)- 5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 526.1, found 526.1. 'H NMR (DMSO-i/i, 300 MHz) δ 11.37(d, 1 H), 9.21 (s, 1 H), 8.62-8.63 (d, 1 H), 7.45-7.49 (m, 8 H), 7.20 (d, 1 H), 7.03( d, 1 H), 6.39-6.42 (m, 1 H), 6.26-6.30 (m, 1 H), 5.22 (s, 2 H), 4.57-4.59 (d, 2 H), 4.19 (s, 2 H).
Example 260: Preparation of 2-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro-6- fluoro- 1 H-indol- 5-yl)methyl)isonicotinamide
Figure imgf000328_0001
To a solution of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-cyano-4-((2-oxopyridin-l(2H)-yl) methyl)benzyl)isonicotinamide (60 mg) in DMF (2 mL) was added NH3OH (2 mL) followed by 30% of H2O2 (0.2 mL). The resultant mixture was stirred for overnight. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by prep-HPLC to give 2-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3- chloro-6-fluoro-lH-indol-5-yl)methyl)isonicotinamide (10 mg, 16%>) as a yellow solid. LRMS (M+H+) m/z calculated 544, found 544. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.95 (s, 1 H), 8.85 (t, 1 H), 8.54 (d, 1 H), 8.40 (t, 1 H), 8.29 (d, 1 H), 7.86-7.92 (m, 2 H), 7.67-7.70 (m, 2 H), 7.24 (d, 1 H), 6.44 (d, 1 H), 5.15 (s, 2 H), 4.54 (d, 2 H), 4.29 (d, 2 H), 1.86 (s, 3 H).
Example 261: Preparation of 2-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro -1H- pyrrolo[2,3-b]pyridin-5-yl)meth l isonicotinamide
Figure imgf000328_0002
To a solution of 2-(3-cyano-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinic acid (150 mg, crude) in DMF (2 mL) was added (3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl) methanamine hydrochloride (61 mg, 0.28 mmol, 1.0 eq) followed by EDCI (65 mg, 0.34 mmol, 1.2 eq), HOBT (46 mg, 0.34 mmol, 1.2 eq) and TEA (85 mg, 0.84 mmol, 3.0 eq). The reaction mixture was stirred for overnight. LCMS showed the SM was consumed. To the mixture was added 85% of NH4OH (1 mL) followed by 30% of I C .The mixture was stirred for 3 h and then concentrated. The residue was purified by prep-HPLC to give2-(3-carbamoyl-4-((2- oxopyridin-l(2H)-yl)methyl) benzyl)-N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)isonicotinamide (28 mg, 19%) as a white solid. LRMS (M+H+) m/z calculated 527, found 527. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.94 (s, 1 H), 9.28 (t, 1 H), 8.61 (d, 1 H), 8.30 (s, 1 H), 8.05 (s, 1 H), 7.87 (s, 1 H), 7.71-7.73 (m, 2 H), 7.67 (s, 1 H), 7.61 (d, 2 H), 7.50 (s, 1 H), 7.41-7.45 (m, 2 H), 7.29 (d, 1 H), 6.88 (d, 1 H), 6.40 (d, 1 H), 6.23 (t, 1 H), 5.21 (s, 2 H), 4.58 (d, 2 H), 4.13 (s, 2 H).
Example 262: Preparation of 2-(3-(aminomethyl)-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3- chloro-6- fluoro-lH- indol-5-yl)methyl)isonicotinamide.
Figure imgf000329_0001
2-(3-(Aminomethyl)-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3- chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide (20 mg, 10%) was prepared as described for 2-(3-chloro-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-N-((3-chloro-6-fluoro- lH-indol-5-yl)methyl)isonicotinamide. LRMS (M+H+) m/z calculated 529, found 529. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.38 (s, 1 H), 9.21 (t, 1 H), 8.61 (d, 1 H), 7.69 (s, 1 H), 7.61-7.64 (m, 2 H), 7.50 (s, 1 H), 7.41-7.45 (m, 2 H), 7.34 (s, 1 H), 7.22 (d, 1 H), 7.08 (d, 1 H), 6.76 (d, 1 H), 6.41 (d, 1 H), 6.22 (t, 1 H), 5.12 (s, 2 H), 4.57 (d, 2 H), 4.10 (s, 2 H), 3.75 (s, 2 H).
Example 263: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2- oxopyridin- 1 (2H)-yl)
Figure imgf000329_0002
To a solution of 3-iodo-4-(2-oxo-2H-pyridin-l-ylmethyl)-benzoic acid methyl ester (2.0 g, 5.42 mmol, 1 eq) in DMSO (10 mL) was added L-proline sodium salt (160 mg , 1.08 mmol, 0.2 eq), Cul (103 mg, 0.54 mmol, 0.1 eq) and CHsSC Na (663 mg, 6.50 mmol, 1.2 eq). The mixture was stirred at 110 °C for 24 h. After cooling to rt the mixture was poured into water and the mixture was extracted with EA. The combined extracts were dried and concentrated. The residue was purified by column chromatography on silica gel (DCM/MeOH = 100/1, v/v) to give 3-methanesulfonyl-4-(2-oxo-2H-pyridin-l-ylmethyl)-benzoic acid methyl ester (460 mg, 26%) as an off white solid.
Figure imgf000329_0003
To a solution of 3-methanesulfonyl-4-(2-oxo-2H-pyridin-l -ylmethyl)-benzoic acid methyl ester (450 mg, 1.4 mmol, 1 eq) and CaCh. (154 mg, 1.4 mmol, 1 eq) in MeOH (15 mL) was added NaBH4 (106 mg, 2.8 mmol, 2 eq) portionwise. The mixture was stirred at rt for 2 h and quenched by the addition of water. MeOH was removed by evaporation and the aqueous phase was extracted with EA. The combined extracts were dried and concentrated to give l-(4-hydroxymethyl-2-methanesulfonyl-benzyl)-lH-pyridin-2-one (390 mg, 95%) as a white solid.
Figure imgf000330_0001
The mixture of l-(4-hydroxymethyl-2-methanesulfonyl-benzyl)-lH-pyridin- 2-one (390 mg, 1.33 mmol, 1 eq) and SOCk (5 mL) was stirred at rt for 1 h. The volatiles were then removed at 40 °C in vacuum and the residue was dissolved in DCM. The mixture was washed with saturated aq. NaHC03, dried and concentrated to give l-(4-chloromethyl-2-methanesul e (390 mg, 94%>) as a white solid.
Figure imgf000330_0002
2-[3-Methanesulfonyl-4-(2-oxo-2H-pyridin-l -ylmethyl)-benzyl]-isonicotinic acid methyl ester (320 mg, 62%) was prepared as described for 2-[6-(2-oxo-2H-pyridin-l-ylmethyl)-pyridin-3-ylmethyl]-isonicotinic acid methyl ester as a yellow solid.
Figure imgf000330_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide (10 mg, 14% yields for 2 steps) was prepared as described for N-(3-chloro- 6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin- 2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 485.1, found 485.1. ¾ NMR (DMSO-ifc, 300 MHz) δ 11.39 (d, 1 H), 9.23 (t, 1 H), 8.63-8.62 (d, 1 H), 7.91-7.77 (m, 3 H), 7.65-7.44 (m, 5 H), 7.24-7.21 (d, 1 H), 6.85-6.82 (d, 1 H), 6.65-6.34 (m, 2 H), 5.43 (s, 2 H), 4.59-4.58 (d, 2 H), 4.24 (s, 2 H), 2.50 (d, 3 H).
Example 264: Preparation of N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(3-(methylsulfonyl)-4- ((2-oxopyridin-l (2H)-yl)methyl)benzyl)iso
Figure imgf000330_0004
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide (30 mg, 44% yields for 2 steps) was prepared as described for N-(3-chloro- 6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin- 2-yl)-pyridin-3-ylmethyl] -nicotinamide as a white solid. LRMS (M+H+) m/z calculated 562.1, found 562.1. ¾ NMR (DMSO-ifc, 300 MHz) δ 11.96 (s, 1 H), 9.33 (t, 1 H), 8.65-8.63 (d, 1 H), 8.30 (d, 1 H), 7.91-7.78 (m, 4 H), 7.68-7.64 (m, 2 H), 7.59-7.50 (m, 2 H), 6.85-6.82 (d, 2 H), 6.45-6.32 (m, 2 H), 5.43 (s, 2 H), 4.60-4.58 (d, 2 H), 4.25 (d, 2 H).
Example 265: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2- oxopyridin- 1 (2H)-yl)methyl)benzyl)isonicot
Figure imgf000331_0001
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide (18 mg, 28% yield for 2 steps) was prepared as described for N-((6-amino- 2,4-dimethylpyridin-3-yl)methyl)-2-((6-methylquinolin-3-yl) methyl)isonicotinamide as an off-white solid. LRMS (M+H+) m/z calculated 532.1, found 532.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 8.63-8.57 (m, 2 H), 7.90-7.85 (m, 2 H), 7.72 (s, 1 H), 7.60-7.50 (m, 3 H), 6.85-6.83 (d, 1 H), 6.45-6.33 (m, 2 H), 6.13 (s, 1 H), 5.68 (s, 2 H), 4.35-4.34 (d, 2 H), 4.23 (s, 2 H), 3.40-3.38 (m, 3 H), 2.30 (s, 3 H), 2.16 (s, 3 H).
Example 266: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)isonicotinamide
Figure imgf000331_0002
N-(( 1 -aminoisoquinolin-6-yl)methyl)-2-(3 -(methylsulfonyl)-4-((2-oxopyridin- 1 (2H)- yl)methyl)benzyl)isonicotinamide (9 mg, 28%> yield for 2 steps) was prepared as described for N-((6-amino- 2,4-dimethylpyridin-3-yl)methyl)-2-((6-methylquinolin-3-yl)methyl) isonicotinamide as an off-white solid. LRMS (M+H+) m/z calculated 554.1, found 554.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.39 (t, 1 H), 8.65-8.64 (d, 1 H), 8.15-8.13 (d, 1 H), 7.92-7.89 (m, 2 H), 7.87-7.79 (m, 2 H), 7.77-7.75 (d, 1 H), 7.68-7.52 (m, 3 H), 7.41-7.39 (dd, 1 H), 6.87-6.83 (m, 2 H), 6.72 (s, 2 H), 6.45-6.42 (d, 1 H), 6.34 (t, 1 H), 5.43 (s, 2 H), 4.62-4.60 (d, 2 H), 4.26 (s, 2 H), 3.40 (s, 3 H).
Example 267: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-phenylpyridin-3- yl)methyl)isonicotinamide
Figure imgf000331_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-phenylpyridin-3-yl)methyl)isonicotinamide (34 mg, 24%> yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 471.1, found 471.1. ¾ NMR (DMSO-ifc, 300 MHz) δ 11.39 (s, 1 H), 9.25 (t, 1 H), 8.66-8.63 (m, 2 H), 8.06-8.02 (dd, 2 H), 7.89-7.87 (d, 1 H), 7.80-7.75 (m, 2 H), 7.67-7.65 (m, 1 H), 7.50-7.40 (m, 5 H), 7.25-7.21 (d, 1 H), 4.61- 4.59 (d, 2 H), 3.85 (s, 2 H).
Example 268: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-methyl-2- (methylsulfonyl)quinolin-3-yl)methyl)iso
Figure imgf000332_0001
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-methyl-2-(methylsulfonyl)quinolin-3- yl)methyl)isonicotinamide (19 mg, 13% yields for 2 steps) was prepared as described for N-(3-chloro-6- fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-yl methyl] -nicotinamide as purple solid. LRMS (M+H+) m/z calculated 537.1, found 537.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.40 (s, 1 H), 9.27-9.24 (t, 1 H), 8.63-8.62 (d, 1 H), 8.31 (s, 1 H), 8.02-8.00 (d, 1 H), 7.82 (s, 1 H), 7.75-7.45 (m, 5 H), 7.24-7.21 (d, 1 H), 4.78 (s, 2 H), 4.60-4.58 (d, 2 H), 3.52 (s, 3 H), 2.52-2.51 (t, 3 H).
Example 269: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-methylquinolin-6- yl)methyl)isonicotinamide
Figure imgf000332_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-methylquinolin-6-yl)methyl)isonicotinamide (16 mg, 15% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 459.1, found 459.1. ¾ NMR (DMSO-tfc, 300 MHz) δ 11.39 (s, 1 H), 9.24 (t, 1 H), 8.66-8.64 (d, 1 H), 8.18-8.16 (d, 1 H), 7.85-7.78 (m, 3 H), 7.65-7.62 (m, 2 H), 7.50-7.36 (m, 3 H), 7.24-7.20 (d, 1 H), 4.59-4.57 (d, 2 H), 4.33 (s, 2 H), 2.62 (s, 3 H).
Example 270: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methylquinolin-6- yl)methyl)isonicotinamide
Figure imgf000332_0003
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methylquinolin-6-yl)methyl)isonicotinamide (30 mg, 20%> yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 434.2, found 434.2. ¾ NMR (CD3OD, 300 MHz) δ 8.64 (d, 1 H), 8.14 (d, 1 H), 8.06 (d, 1 H), 7.88 (d, 1 H), 7.77-7.59 (m, 6 H), 7.47 (d, 1 H), 7.37 (d, 1 H), 6.90 (d, 1 H), 4.69 (s, 2 H), 4.38 (s, 2 H) , 2.68 (s, 3 H). Example 271: Preparation of N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((2-methylquinolin-6- yl)methyl)isonicotinamide
Figure imgf000333_0001
N-((6-amino-2-methylpyridin-3 -yl)methyl)-2-((2-methylquinolin-6-yl)methyl)isonicotinamide (29 mg, 14% yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin- l(2H)-yl)methyl)benzyl)isonicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 398.1, found 398.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.02 (t, 1 H), 8.65-8.63 (d, 1 H), 8.18-8.16 (d, 1 H), 7.86-7.63 (m, 5 H), 7.38-7.36 (d, 2 H), 7.26-7.24 (d, 1 H), 6.25-6.23 (d, 1 H), 5.77-5.76 (d, 2 H), 4.33-4.29 (m, 4 H), 2.63 (s, 3 H), 2.23 (s, 3 H).
Example 272: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methylquinolin-6- yl)methyl)isonicotinamide
Figure imgf000333_0002
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methylquinolin-6-yl)methyl)isonicotinamide (45 mg, 16%) was prepared as described for N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-cyano-2- oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 411.9, found 411.9. ¾ NMR (DMSO-ifc, 300 MHz) δ 8.59-8.65 (m, 2 H), 8.17 (d, 1 H), 7.83 (d, 1 H), 7.75 (d, 2 H), 7.58-7.64 (m, 2 H), 7.37 (d, 1 H), 6.11 (s, 1 H), 5.67 (s, 2 H), 4.30-4.34 (m, 3 H), 2.61 (d, 3 H), 2.29 (s, 3 H), 2, 15 (s, 3 H). Example 273: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6-methylquinolin-3- yl)methyl)isonicotinamide
Figure imgf000333_0003
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6-methylquinolin-3-yl)methyl)isonicotinamide (29 mg, 21% yield for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2- oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 412.1, found 412.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 8.72 (s, 1 H), 8.57-8.52 (d, 2 H), 8.00 (s, 1 H), 7.80-7.58 (m, 2 H), 7.53-7.44 (m, 3 H), 6.05 (s, 1 H), 5.62 (s, 2 H), 4.26 (s, 4 H), 2.42-2.39 (m, 3 H), 2.22 (s, 3 H), 2.07 (s, 3
H).
Example 274: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-methylquinolin-3- yl)methyl)isonicotinamide
Figure imgf000334_0001
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-methylquinolin-3-yl)methyl)isonicotinamide (27 mg, 18% yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 434.2, found 434.2. ¾ NMR (CD3OD, 400 MHz) δ 8.74 (d, 1 H), 8.66 (d, 1 H), 8.11 (s, 1 H), 8.07 (d, 1 H), 7.88 (d, 1 H), 7.83 (s, 1 H), 7.72 (d, 1 H), 7.69 (d, 1 H), 7.61 (s, 2 H), 7.51 (d, 1 H), 7.49 (d, 1 H), 6.92 (d, 1 H), 4.71 (s, 2 H), 4.41 (s, 2 H) , 2.51 (s, 3 H).
Example 275: Preparation of N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-2-((6-methylquinolin-3-yl)methyl) isonicotinamide
Figure imgf000334_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-methyl quinolin-3- yl) methyl)isonicotinamide (62 mg, 40%) was prepared as described for (5- {5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-3- ylmethyl}-pyridin-2-ylmethyl)-carbamic acid tert-butyl ester as an off-white solid. LRMS (M+H+) m/z calculated 459, found 459. ¾ NMR (DMSO-tfc, 400 MHz) δ 11.39 (s, 1 H), 9.24 (t, 1 H), 8.80 (s, 1 H), 8.64 (d, 1 H), 8.09 (s, 1 H), 7.87 (d, 1 H), 7.81 (s, 1 H), 7.65-7.67 (m, 2 H), 7.50-7.55 (m, 2 H), 7.45 (d, 1 H), 7.23 (d, 1 H), 4.59 (d, 2 H), 4.37 (s, 2 H), 2.48 (s, 3 H).
Example 276: Preparation of N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((6-methylquinolin-3- yl)methyl)isonicotinamide
Figure imgf000334_0003
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-fluoroquinolin-3-yl)methyl)isonicotinamide (30 mg, 20%> yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 438.2, found 438.2. ¾ NMR (CD3OD, 300
MHz) δ 8.81 (d, 1 H), 8.66 (d, 1 H), 8.20 (d, 1 H), 8.08 (d, 1 H), 8.05 (dd, 1 H), 7.83 (s, 1 H), 7.71 (d, 1 H),
7.70 (d, 1 H), 7.62 (s, 1 H), 7.57-7.47 (m, 3 H), 6.93 (d, 1 H), 4.72 (s, 2 H), 4.44 (s, 2 H).
Example 278: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6-fluoroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000335_0001
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6-fluoroquinolin-3-yl)methyl)isonicotinam (35 mg, 25% yield for 2 steps) was prepared as described for N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6- methylquinolin-3-yl)methyl)isonicotinamide as an off- white solid. LRMS (M+H+) m/z calculated 416.1, found 416.1. ¾ NMR (DMSO- 300 MHz) δ 8.87-8.86 (d, 1 H), 8.66-8.58 (m, 2 H), 8.18 (d, 1 H), 8.06- 8.02 (m, 1 H), 7.78-7.72 (m, 2 H), 7.64-7.58 (m, 2 H), 6.11 (s, 2 H), 5.68 (s, 2 H), 4.36-4.33 (m, 4 H), 2.30 (s, 3 H), 2.16 (s, 3 H).
Example 279: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-fluoroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000335_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-fluoroquinolin-3-yl)methyl)isonicotinamide (42 mg, 26.8%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2- yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 463.1, found 463.1. Ή NMR (DMSO- d6, 300 MHz) δ 11.41 (s, 1 H), 9.26 (t, 1 H), 8.88 (d, 1 H), 8.65 (d, 1 H), 8.10 (s, 1 H), 8.02-8.07 (m, 1 H), 7.82 (s, 1 H), 7.74 (dd, 1 H), 7.61-7.67 (m, 2 H), 7.51 (s, 1 H), 7.45 (d, 1 H), 7.22 (d, 1 H), 4.59 (d, 2 H), 4.39 (s, 2 H).
Example 280: Preparation of N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((6-fluoroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000335_0003
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((6-fluoroquinolin-3-yl)methyl)isonicotinamide (18 mg, 13.2%) was prepared as described for N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((5-fluoro-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 401.9, found 401.9. lH NMR (CD3OD, 300 MHz) δ 8.80 (d, 1 H), 8.63 (d, 1 H), 8.20 (s, 1 H), 8.01-8.04 (m, 1 H), 7.78 (s, 1 H), 7.63-7.64 (m, 1 H), 7.52- 7.58 (m, 3 H), 7.40 (d, 1 H), 4.43 (d, 4 H), 2.38 (s, 3 H).
Example 281: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((7-fluoroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000336_0001
N-((6-amino-2,4-dimethylpyridin-3-yl)m (35 mg,
25%) was prepared as described for N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-cyano-2- oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 415.9, found 415.9. ¾ NMR (DMSO-i ,5, 300 MHz) δ 8.92 (d, 1 H), 8.65 (t, 1 H), 8.60 (d, 1 H), 8.26 (s, 1 H), 8.04 (dd, 1 H), 7.79 (s, 1 H), 7.73 (dd, 1 H), 7.61 (d, 1 H), 7.51-7.55 (m, 1 H), 6.13 (s, 1 H), 5.68 (s, 2 H), 4.35-4.37 (m, 4 H), 2.31 (s, 3 H), 2.17 (s, 3 H).
Example 282: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((7-fluoroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000336_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((7-fluoroquinolin-3-yl)methyl)isonicotinamide (45 mg, 28.7%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2- yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 462.8, found 462.8. Ή NMR (DMSO- d6, 300 MHz) δ 11.39-1 1.42 (m, 1 H), 9.26 (t, 1 H), 8.92 (s, 1 H), 8.64 (d, 1 H), 8.27 (s, 1 H), 8.04 (dd, 1 H), 7.82 (s, 1 H), 7.65-7.74 (in, 2 H), 7.43-7.55 (m, 3 H), 7.22 (d, 1 H), 4.59 (d, 2 H), 4.38 (s, 2 H).
Example 283: Preparation of N-((l -amino isoquinolin-6-yl)methyl)-2-((7-fluoroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000336_0003
N-((l-aminoisoquinolin-6-yl)methyl)-2-((7-fluoroquinolin-3-yl)methyl)isonicotinamide (16 mg, 10.8%) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 437.8, found 437.8. Ή NMR (CD3OD, 400 MHz) δ 8.72 (d, 1 H), 8.52 (d, 1 H), 8.09 (s, 1 H), 7.93 (d, 1 H), 7.77-7.80 (m, 1 H), 7.70 (s, 1 H), 7.56-7.58 (in, 2 H), 7.47-7.50 (m, 2 H), 7.35 (d, 2 H), 7.26-7.31 (m, 1 H), 6.78 (d, 1 H), 4.58 (s, 2 H), 4.29 (s, 2 H). Example 284: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(quinolin-3- ylmethyl)isonicotinamide
Figure imgf000336_0004
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(quinolin-3-ylmethyl)isonicotinami (24 mg, 17% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2- yl)-pyridin-3-ylmethyl]-nicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 445.1, found 445.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.40 (s, 1 H), 9.26 (t, 1 H), 9.01 (s, 1 H), 8.66-8.65 (d, 1 H), 8.40 (s, 1 H), 8.05-8.00 (t, 2 H), 7.85 (s, 1 H), 7.80 (t, 1 H), 7.69-7.66 (m, 2 H), 7.51-7.44 (m, 2 H), 7.24-7.21 (d, 1 H), 4.60- 4.59 (d, 2 H), 4.44 (s, 2 H).
Example 285: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-chloroquinolin-3-yl) methyl)isonicotinamide
Figure imgf000337_0001
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-chloro quinolin-3-yl)methyl) isonicotinamide (12 mg, 15%) was prepared as described for (5- {5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-3- ylmethyl}-pyridin-2-ylmethyl)-carbamic acid tert-butyl ester as an off- white solid. LRMS (M+H+) m/z calculated 479, found 479. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.25 (t, 1 H), 8.92 (d, 1 H), 8.64 (d, 1 H), 8.20 (s, 1 H), 8.08 (d, 1 H), 8.00 (d, 1 H), 7.82 (s, 1 H), 7.71 (d, 1 H), 7.66 (d, 2 H), 7.50 (d, 2 H), 7.45 (d, 1 H), 7.22 (d, 1 H), 4.59 (d, 2 H), 4.40 (s, 2 H).
Example 286: Preparation of
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2 -yl)methyl)isonicotinamide
Figure imgf000337_0002
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methylquinolin-7-yl)methyl)isonicotinamide (35 mg, 22%>) was prepared as described for N-((l -aminoisoquinolin-6-yl)methyl)-2- (4-((5-cyano-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 434, found 434.¾ NMR (DMSO-<¾ 400 MHz) δ 9.39 (t, 1 H), 8.67 (d, 1 H), 8.18 (d, 1 H), 8.13 (d, 1 H), 7.80-7.85 (m, 3 H),7.75 (d, 1 H), 7.68 (d, 1 H), 7.54 (s, 1 H), 7.47 (d, 1 H), 7.35- 7.40 (m, 2 H), 6.84 (d, 1 H), 6.72 (s, 2 H), 4.60 (d, 2 H), 4.37 (s, 2 H), 2.62 (s, 3 H).
Example 287: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-methylquinolin-7- yl)methyl)isonicotinamide
Figure imgf000337_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-methylquinolin-7-yl)methyl)isonicotinamide (25 mg, 15%)) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)- pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 459, found 459. ¾ NMR (DMSO-<¾ 300
MHz) δ 11.40 (br, 1 H), 9.26 (t, 1 H), 8.65 (d, 1 H), 8.17 (d, 1 H), 7.79-7.84 (m, 3 H),7.65 (d, 1 H), 7.42- 7.50
(m, 3 H), 7.35 (d, 1 H), 7.22 (d, 2 H), 4.57 (d, 2 H), 4.35 (s, 2 H), 2.62 (s, 3 H).
Example 288: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methylquinolin-7- yl)methyl)isonicotinamide
Figure imgf000338_0001
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methylquinolin-7-yl)methyl)isonicotinamide (10 mg, 7%) was prepared as described for N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-cyano-2- oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 412, found 412. ¾ NMR (DMSO-ifc, 300 MHz) δ 8.59 - 8.64 (m, 2 H), 8.17 (d, 1 H), 7.82 (d, 1 H), 7.76 (d, 2 H), 7.60 (d, 1 H), 7.45 (d, 1 H), 7.35 (d, 1 H), 6.10 (s, 1 H), 5.67 (s, 2 H), 4.33 (s, 4 H), 2.62 (s, 3 H), 2.29 (s, 3 H), 2.15 (s, 3 H).
Example 289: Preparation of N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-(2-cyano-quinolin-6-ylmethyl)- isonicotinamide
Figure imgf000338_0002
N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-(2-cyano-quinolin-6-ylmethyl)-isonicotinamide (90 mg, 22% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 570.1, found 570.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.41 (s, 1 H), 9.26 (t, 1 H), 8.60-8.65 (m, 2 H), 8.00-8.08 (m, 3 H), 7.87-7.89 (dd, 1 H), 7.81 (s, 1 H), 7.66-7.67 (dd, 1 H), 7.50-7.51 (d, 1 H), 7.43-7.45 (d, 1 H), 7.21-7.24 (d, 1 H), 4.58- 4.59 (d, 2 H), 4.42 (s, 2 H).
Example 290: Preparation of N-(6-amino-2,4-dimethyl-pyridin-3-ylmethyl)-2-(2-cyano-quinolin-6-ylmethyl)- isonicotinamide
Figure imgf000338_0003
N-(6-amino-2,4-dimethyl-pyridin-3-ylmethyl)-2-(2-cyano-quinolin-6-ylmethyl)-isonicotinamide (90 mg, 26% yields for 2 steps) was prepared as described for N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5- cyano-2-oxopyridin-l (2H)-yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 423.1, found 423.1. 'H NMR (DMSO-ifc, 400 MHz) δ 8.60-8.64 (m, 3 H), 8.00-8.07 (m, 3 H), 7.86-7.88 (m, 1 H), 7.78 (s, 1 H), 7.61-7.62 (d, 1 H), 6.12 (s, 1 H), 5.68 (s, 2 H), 4.33-4.40 (m, 4 H), 2.30 (s, 3 H), 2.16 (s, 3 H).
Example 291: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylisoquinolin-6- yl)methyl)isonicotinamide
Figure imgf000339_0001
To a solution of 4-bromo-benzylamine (10. 0 g, 54 mmol, 1.0 eq) in DCE (100 mL) was added 1,1- dimethoxy-propan-2-one (7.0 g, 59 mmol, 1.1 eq) and MgS04 (20 g). The mixture was stirred at 40 °C overnight. Then to the mixture was added NaB¾CN (4.08 g, 64.8 mmol, 1.2 eq). After stirring at rt for 5 h the mixture was filtered. The filtrate was concentrated to give a yellow oil. Chlorosulfonic acid (30 mL) was cooled to -10 °C and the above crude product was added dropwise. The reaction mixture was heated to 100 °C for 10 min, then cooled and poured into ice. The mixture was neutralized with 2M NaOH and extracted with EA. The combined extracts were dried and concentrated. The residue was purified by silica gel
chromatography (PE/EA = 2/1, v/v) to afford 6-bromo-3-methyl-isoquinoline (4.0 g, 34% yield for 3 steps) as a yellow solid.
Figure imgf000339_0002
An autoclave vessel was charged with 6-bromo-3-methyl-isoquinoline (4.0 g, 18 mmol), PdidppfjCb. (735 mg, 0.9 mmol, 0.05 eq) and triethylamine (5.0 mL, 36 mmol, 2 eq) in 40 mL of methanol. The vessel was purged with nitrogen three times and carbon monoxide three times. The vessel was pressurized to 3 MPa with carbon monoxide and heated to 00 °C. The reaction was thus stirred overnight, then allowed to cool to room temperature. The resulting solution was concentrated and purified by flash chromatography on silica gel (PE/EA = 1/1, v/v) to afford 3-methyl-isoquinoline-6-carboxylic acid methyl ester (3.4 g, 94%) as a white solid.
Figure imgf000339_0003
To a solution of 3-methyl-isoquinoline-6-carboxylic acid methyl ester (3.3 g, 16.42 mmol, 1 eq) in dry THF (100 mL) was added LiAlH(t-BuO)3 (12.5 g, 45.25 mmol, 3 eq). The resulting mixture was stirred at 60 °C for 5 h and then quenched by the addition of water. The mixture was extracted with EA. The combined extracts were dried and concentrated. The residue was purified by silica gel chromatography (PE/EA = 1/1, v/v) to afford (3-methyl-isoquinolin-6-yl)-me white solid.
Figure imgf000339_0004
To (3-methyl-isoquinolin-6-yl)-methanol (1.5 g, 8.67 mmol, 1 eq) was added SOCh (9 mL) and the mixture was stirred at rt for 3 h. The volatiles were then removed at 40 °C under vacuum and the residue was dissolved in DCM. The mixture was washed with saturated aq. NaHC03, dried and concentrated to give 6- chloromethyl-3-methyl-isoquinoline (1.4 g, 85%) as a white solid. 2-(3-Methyl-isoquinolin-6-ylmethyl)-isonicotinic acid methyl ester (1.0 g, 47%) was prepared as described for 2-[6-(2-oxo-2H-pyridin-l-y thyl ester.
Figure imgf000340_0001
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylisoquinolin-6-yl)methyl)isonicotinamide (20 mg, 14%> yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((5-cyano-2- oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 434.2, found 434.2. ¾ NMR (CD3OD, 400 MHz) δ 9.06 (s, 1 H), 8.66 (d, 1 H), 8.08 (d, 1 H), 7.97 (d, 1 H), 7.80 (s, 1 H), 7.71 (s, 2 H), 7.70 (s, 1 H), 7.62 (s, 1 H), 7.56 (s, 1 H), 7.50 (d, 1 H), 7.47 (d, 1 H), 6.92 (d, 1 H), 4.71 (s, 2 H), 4.11 (s, 2 H), 2.64 (s, 3 H).
Example 292: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methylisoquinolin-6- yl)methyl)isonicotinamide
Figure imgf000340_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methylisoquinolin-6-yl)methyl)isonicotinamide (43 mg, 28%) yields for 2 steps) was prepared as discrbed for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 459.1 , found 459.1. ¾
NMR (DMSO-ifc, 400 MHz) δ 11.42 (s, 1 H), 9.26 (t, 1 H), 9.15 (s, 1 H), 8.67 (d, 1 H), 8.00 (dd, 1 H), 7.79 (s, 1 H), 7.72 (s, 1 H), 7.67 (d, 1 H), 7.58 (s, 1 H), 7.53 (d, 1 H), 7.52 (s, 1 H), 7.46 (d, 1 H), 7.24 (d, 1 H), 4.60 (d, 2 H), 4.36 (s, 2 H), 2.59 (s, 3 H).
Example 293: Preparation of N-((l -aminoisoquinolin-6-yl)methyl)-2-((2-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide
Figure imgf000340_0003
N-(( 1 -aminoisoquinolin-6-yl)methyl)-2-((2-(methylsulfonyl)quinolin-6-yl)methyl)isonicotinamide (34 mg, 34%o yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((5-cyano-2- oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 498.2, found 498.2. ¾ NMR (CD3OD, 400 MHz) δ 8.66 (d, 1 H), 8.49 (d, 1 H), 8.06 (d, 1 H), 8.03 (d, 1 H), 8.02 (s, 1 H), 7.88 (s, 1 H), 7.81 (s, 1 H), 7.78 (d, 1 H), 7.69 (d, 1 H), 7.67 (d, 1 H), 7.59 (s, 1 H), 7.46 (d, 1 H), 6.88 (d, 1 H), 4.69 (s, 2 H), 4.43 (s, 2 H), 3.37 (s, 3 H).
Example 294: Preparation of N-((3-chloro-6-fluoro-l H-indol-5-yl)methyl)-2-((2-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide
Figure imgf000341_0001
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-(methylsulfonyl)quinolin-6-yl)methyl)isonicotinami (24 mg, 23% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5- oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl] -nicotinamide. LRMS (M+H+) m/z calculated 523.1, found 523.1. ¾ NMR (CD3OD, 400 MHz) δ 8.53 (d, 1 H), 8.47 (d, 1 H), 8.02 (d, 1 H), 7.99 (d, 1 H), 7.84 (s, 1 H), 7.75 (d, 1 H), 7.68 (s, 1 H), 7.56 (d, 1 H), 7.40 (d, 1 H), 7.13(s, 1 H), 7.02 (s, 1 H), 4.58 (s, 2 H), 4.36 (s, 2 H), 3.25 (s, 3 H).
Example 295: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-((4-cyanoquinolin-6- yl)methyl)isonicotinamide
Figure imgf000341_0002
A mixture of quinoline-6-carboxylic acid methyl ester (10 g, 53.5 mmol, 1 eq) and m-CPBA (18.4 g, 0.106 mol, 2 eq) in DCM (50 mL) was stirred at rt overnight. Saturated aq. NaHCC (40 mL) was added to the reaction mixture and it was stirred for 30 min. The organic layer was separated, dried, filtered and
concentrated to obtain a residue, which was re-crystallized by EA (5 mL) to afford 1 -oxy-quinoline-6- carboxylic acid methyl ester (8.0 g, 74%>) as a light yellow solid.
Figure imgf000341_0003
To l-oxy-quinoline-6-carboxylic acid methyl ester (4.0 g, 19.7 mmol, 1 eq) was added phosphoryl trichloride (20 mL). The resulting mixture was then stirred at rt under N2 for 2 h. The volatiles were then removed under vacuum and the residue was dissolved in DCM. The mixture was washed with saturated aq. NaHCCb, dried and concentrated. The residue was purified by silica gel chromatography (PE/EA = 10/1, v/v) to afford 2- chloro-quinoline-6-carboxylic acid methyl ester (1.2 g, 28%>) and 4-chloro-quinoline-6-carboxylic acid methyl ester (2.5 g, 57%).
Figure imgf000341_0004
To a solution of 4-chloro-quinoline-6-carboxylic acid methyl ester (2.2 g, 10 mmol, 1 eq) was added LiAlH(t- BuO)3 (7.62 g, 30 mmol, 3 eq). The resulting mixture was stirred at 60 °C for 2 h and then quenched by the addition of water. The mixture was extracted with EA. The combined extracts were dried and concentrated. The residue was purified by silica gel chromatography (PE/EA = 1/1, v/v) to afford (4-chloro-quinolin-6-yl)- methanol (1.54 g, 80%>) as a yellow solid. 6-Hydroxymethyl-quinoline-4-carbonitrile (1.1 g, 77%) was prepared as described for as 1 -(4-hydroxymethyl- 2-methanesulfonyl-benzyl)-lH-pyridin-2-one as an off-white solid.
Figure imgf000342_0001
6-Chloromethyl-quinoline-4-carbonitrile (1.0 g, 83%>) was prepared as described for as 1 -(4-chloromethyl-2- methanesulfonyl-benzyl)-lH-pyridin-2-one as a white solid.
Figure imgf000342_0002
2-(4-Cyano-quinolin-6-ylmethyl)-isonicotinic acid methyl ester (1.0 g, 67%>) was prepared as described for 2- [6-(2-oxo-2H-pyridin-l-ylmethyl) ester as a yellow solid.
Figure imgf000342_0003
N-((l-aminoisoquinolin-6-yl)methyl)-2-((4-cyanoquinolin-6-yl)methyl)isonicotinamide (43 mg, 29%> yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 445.2, found 445.2. ¾ NMR (CD3OD, 300 MHz) δ 9.40 (t, 1 H), 9.06 (d, 1 H), 8.69 (d, 1 H), 8.15-8.11 (m, 3 H), 8.06 (s, 1 H), 7.92 (d, 1 H), 7.87 (s, 1 H), 7.77 (d, 1 H), 7.71 (d, 1 H), 7.56 (s, 1 H), 7.41 (d, 1 H), 6.85 (d, 1 H), 6.73 (s, 2 H), 4.62 (d, 2 H) , 4.50 (s, 2 H).
Example 296: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-cyanoquinolin-6- yl)methyl)isonicotinamide
Figure imgf000342_0004
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-cyanoquinolin-6-yl)methyl)isonicotinamide (45 mg, 29%> yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 470.1, found 470.1. ¾ NMR (DMSO, 400 MHz) δ 11.42 (s, 1 H), 9.28 (t, 1 H), 9.07 (d, 1 H), 8.68 (d, 1 H), 8.14 (d, 1 H), 8.12 (s, 1 H), 8.06 (s, 1 H), 7.91 (d, 1 H), 7.86 (s, 1 H), 7.68 (d, 1 H), 7.51 (d, 1 H), 7.46 (d, 1 H), 7.24 (d, 1 H), 4.60 (d, 1 H), 4.49 (s, 2 H).
Example 297: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((4-cyanoquinolin-6- yl)methyl)isonicotinamide
Figure imgf000343_0001
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((4-cyanoquinolin-6-yl)methyl)isonicotinamide (23 mg, 17% yields for 2 steps) was prepared as described for N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6- methylquinolin-3-yl)methyl)isonicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 423.2, found 423.2. 'H NMR (CD3OD, 300 MHz) δ 8.98 (d, 1 H), 8.63 (d, 1 H), 8.10 (d, 1 H), 8.07 (s, 1 H), 7.93 (d, 1 H), 7.86 (dd, 1 H), 7.77 (s, 1 H), 7.63 (dd, 1 H), 6.29 (s, 1H), 4.50 (s, 2 H), 4.49 (s, 2 H), 2.38 (s, 3 H), 2.26 (s, 3 H).
Example 298: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-((7-chloroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000343_0002
To a solution of 7-chloro-quinoline-3-carboxylic acid ethyl ester (3.5 g, 14.90 mmol, 1.0 eq) in EtOH (60 mL) was added hydrazine monohydrate (7.2 mL, 149 mmol, 10 eq). The reaction mixture was stirred at 80 °C for 2 h, and then concentrated under reduced pressure. Water was added to the reaction flask and the solid was filtered and washed with cold water. The solid was dried in air and then dissolved in pyridine (30 mL). To the mixture was added TsCl (3.4 g, 17.90 mmol, 1.2 eq). After stirring at rt for 1 h, the mixture was concentrated in vacuo. The residue was poured into water and the resulting precipitate was collected by filtration to give N- (7-chloro-quinoline-3-carbonyl)-N'-(to g, 90%) as a yellow solid.
Figure imgf000343_0003
A mixture of 7 N-(7-chloro-quinoline-3-carbonyl)-N'-(toluene-4-sulfonyl)- hydrazine (5.0 g, 13.30 mmol, 1.0 eq) and Na2C03 (4.24 g, 40 mmol, 3 eq) in ethylene glycol (30 mL) was heated at 160 °C for 20 min. After cooling to rt the mixture was diluted with water and extracted with Et20. The combined extracts were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (PE/EA = 1/1, v/v) to give (7-chloro-quinolin-3-yl)-metha a yellow solid.
Figure imgf000343_0004
7-Chloro-3-chloromethyl-quinoline (550 mg, 84%>) was prepared as described for as l-(4-chloromethyl-2- methanesulfonyl-benzyl)-lH-pyridin-2-
Figure imgf000343_0005
2-(7-Chloro-quinolin-3-ylmethyl)-isonicotinic acid methyl ester (400 mg, 49%) was prepared as described for 2-[6-(2-oxo-2H-pyridin-l-ylmethyl) methyl ester as a yellow solid.
Figure imgf000344_0001
N-((l-aminoisoquinolin-6-yl)methyl)-2-((7-chloroquinolin-3-yl)methyl)isonicotinamide (34 mg, 23% yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 454.1, found 454.1. ¾ NMR (DMSO-<¾, 400 MHz) δ 9.42 (d, 1 H), 8.96 (s, 1 H), 8.68 (d, 1 H), 8.20 (s, 1 H), 8.15 (d, 1 H), 8.05 (s, 1 H), 8.02 (d, 1 H), 7.86 (s, 1 H), 7.78 (d, 1 H), 7.70 (d, 1 H), 7.63 (dd, 1 H), 7.56 (s, 1 H), 7.42 (d, 1 H), 6.86 (d, 1 H), 4.63 (d, 2 H), 4.42 (s, 2 H).
Example 299: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((7-chloroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000344_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((7-chloroquinolin-3-yl)methyl)isonicotinamide (35 mg, 23%> yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo- pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 479.1, found 479.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.42 (s, 1H), 9.27 (t, 1 H), 8.95 (d, 1 H), 8.66 (d, 1 H), 8.27 (s, 1 H), 8.04 (d, 1 H), 8.01 (d, 1 H), 7.83 (s, 1 H), 7.67 (d, 1 H), 7.62 (dd, 1 H), 7.52 (d, 1 H), 7.46 (d, 1 H), 7.25 (d, 1 H), 4.60 (d, 2 H), 4.40 (s, 2 H).
Example 300: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((7-chloroquinolin-3- yl)methyl)isonicotinamide
Figure imgf000344_0003
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((7-chloroquinolin-3-yl)methyl)isonicotinamide (10 mg, 7%> yields for 2 steps) was prepared as described for N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6- methylquinolin-3-yl)methyl)isonicotinamide as a white solid. LRMS (M+H+) m/z calculated 432.2, found 432.2. ¾ NMR (CD3OD, 400 MHz) δ 8.87 (d, 1 H), 8.63 (d, 1 H), 8.25 (s, 1 H), 8.00 (s, 1 H), 7.91 (d, 1 H), 7.78 (s, 1 H), 7.63 (d, 1 H), 7.60 (dd, 1 H), 6.32 (s, 1 H), 4.53 (d, 2 H), 4.44 (s, 2 H), 2.41 (s, 3 H), 2.29 (s, 3 H).
Example 301: Preparation of methyl l -((5-(((3-chloro-6-fluoro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3- yl)methyl)-lH-pyrazole-4-carboxylate
Figure imgf000345_0001
To a solution of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((4-cyano-lH- pyrazol-1 - yl)methyl)nicotinamide (100 mg, 0.25 mmol, 1 eq) in MeOH (1 mL) was added HCI/MeOH solution. The mixture was stirred at rt for 5 h and concentrated in vacuo. The residue was purified by prep-HPLC to give methyl l -((5-(((3-chloro-6-fluoro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-yl)methyl)-lH-pyrazole-4- carboxylate (9.1 mg, 8%) as a white solid. LRMS (M+H+) m/z calculated 442.1, found 442.1. ¾ NMR (CD3OD, 400 MHz) δ 8.96 (d, 1 H), 8.63 (d, 1 H), 8.20 (s, 1 H), 8.16 (t, 1 H), 7.93 (s, 1 H), 7.54 (d, 1 H), 7.25 (s, 1 H), 7.15 (d, 1 H), 5.48 (s, 2 H), 4.72 (s, 2 H), 3.82 (s, 3 H).
Example 302: Preparation of 5-((4-(acetamidomethyl)-lH-pyrazol-l-yl)methyl)-N-((3-chloro-6-fluoro-lH- indol-5-yl)methyl)nicotinamide
Figure imgf000345_0002
5-((4-(Acetamidomethyl)- 1 H-pyrazol- 1 -yl)methyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5 -yl)methyl)nicotinamide (63 mg, 44% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5- [6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 455.1, found 455.1. ¾ NMR (CD3OD, 400 MHz) δ 8.81 (d, 1 H) 8.44 (d, 1 H), 7.99 (s, 1 H), 7.60 (s, 1 H), 7.42 (d, 1 H), 7.37 (s, 1 H), 7.02 (d, 1 H), 5.29 (s, 2 H), 4.59 (s, 2 H), 4.10 (s, 2 H), 1.81 (s, 3 H).
Example 303: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((4-((N- cyclopropylacetamido)methyl)- 1 H-pyrazol- 1 -yl)methyl)nicotinamide
Figure imgf000345_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((4-((N-cyclopropylacetamido)methyl)-lH-pyrazol-l- yl)methyl)nicotinamide (14 mg, 8.5% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro- lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)- pyridin-3-ylmethyl] -nicotinamide as a white solid. LRMS (M+H+) m/z calculated 495.2, found 495.2. ¾ NMR (CD3OD, 400 MHz) δ 8.82 (d, 1 H), 8.44 (d, 1 H), 7.94 (s, 1 H), 7.63 (s, 1 H), 7.42 (d, 1 H), 7.38 (s, 1 H), 7.13 (s, 1 H), 7.03 (d, 1 H), 5.31 (s, 2 H), 4.60 (s, 2 H), 4.32 (s, 2 H), 2.52-2.50 (m, 1 H), 2.07 (s, 3 H), 0.80-0.71 (m, 4 H). Example 304: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-((N- cyclopropylacetamido)methyl)-lH-pyrazol-l -yl)methyl)isonicotinamide
Figure imgf000346_0001
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-(^
yl)methyl)isonicotinamide (50 mg, 30% yields for 2 steps) was prepared as described for N-(3-chloro-6- fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)- pyridin-3-ylmethyl] -nicotinamide as a white solid. LRMS (M+H+) m/z calculated 495.2, found 495.2. ¾ NMR (CDC13, 400 MHz) δ 8.53 (d, 1 H), 7.52-7.43 (m, 4 H), 7.26 (s, 1 H), 7.07 (s, 1 H), 6.89 (d, 1 H), 5.31 (s, 2 H), 4.66 (s, 2 H), 4.34 (s, 2 H), 3.46 (s, 1 H), 2.59- 2.56 (m, 1 H), 2.14 (s, 3 H), 0.88-0.85 (m, 2 H), 0.77-0.75 (m, 2 H).
Example 305: Preparation of 2-((4-(acetamidomethyl)-lH-pyrazol-l-yl)methyl)-N-((3-chloro-6-fluoro-lH- indol-5-yl)methyl)isonicotinamide
Figure imgf000346_0002
2-((4-(Acetamidomethyl)-lH-pyrazol-l-yl)methyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide (50 mg, 35% yields for 2 steps) was prepared as described for N-(3-chloro-6- fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 455.1, found 455.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.38 (s, 1 H), 9.27 (t, 1 H), 8.67 (d, 1 H), 8.13 (d, 1 H), 7.73 (d, 2 H), 7.45 (s, 1 H), 7.43 (s, 1 H), 7.38 (d, 1 H), 7.36 (s, 1 H), 7.21 (d, 1 H), 5.43 (s, 2 H), 4.59 (d, 2 H), 4.09 (d, 2 H), 1.80 (s, 3 H).
Example 306: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(l-methyl-lH-pyrazol-4- yl)benzyl)isonicotinamide
Figure imgf000346_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(l -methyl-lH-pyrazol-4-yl)benzyl)isonicotinamide (63 mg, 63%>) was prepared as described for 2-(3-fluoro-4-formyl-phenyl)-pyrrole-l -carboxylic acid tert-butyl ester as a yellow solid. LRMS (M+H+) m/z calculated 474.1, found 474.1. ¾ NMR (CD3OD, 400 MHz) δ 8.62 (d, 1
H), 7.91 (s, 1 H), 7.79 (s, 1 H), 7.74 (s, 1 H), 7.65 (d, 1 H), 7.52 (d, 1 H), 7.49 (s, 1 H), 7.41 (d, 1 H), 7.29 (t, 1
H), 7.25 (s, 1 H), 7.14 (d, 1 H), 7.12 (d, 1 H), 4.71 (d, 2 H), 4.23 (d, 2 H), 3.92 (s, 3 H).
Example 307, 308, 309, 310, 311, 312, 313, 314, 315, 316 were prepared as described for N-((3-chloro-6- fluoro-lH-indol-5-yl)methyl)-2-(3-(l-methyl-lH-pyrazol-4-yl)benzyl)isonicotinamide. Example 317: Preparation of 2-(3-(lH-pyrazol-l-yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide
Figure imgf000347_0001
To a solution of 2-(3-(lH-pyrazol-3-yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide (100 mg, 0.21 mmol, 1 eq) and lH-pyrazole (18 mg, 0.26 mmol, 1.2 eq) in dry DMF (4 mL) was added Cu20 (6.0 mg, 0.04 mmol, 0.2 eq) and Cs2C03 (139 mg, 0.42 mmol, 2 eq). The mixture was stirred at 90 °C for 3 h under nitrogen atmosphere. After cooling to rt, the solvent was removed in vacuo. The residue was purified by prep-HPLC to afford 2-(3-(lH-pyrazol-l -yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl) isonicotinamide (9 mg, 9%) as a white solid. LRMS (M+H+) m/z calculated 461.1, found 461.1. ¾ NMR (CD3OD, 400 MHz) δ 8.63 (d, 1 H), 8.19 (d, 1 H), 7.76 (s, 1 H), 7.71 (d, 1 H), 7.69 (d, 1 H), 7.66 (d, 1 H), 7.60 (d, 1 H), 7.54 (d, 1 H), 7.43 (t, 1 H), 7.26 (d, 1 H), 7.25 (s, 1 H), 7.14 (d, 1 H), 6.52 (t, 1 H), 4.71 (s, 2 H), 4.30 (s, 2 H).
Example 318: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(2-oxopyridin-l(2H)- yl)benzyl)isonicotinamide
Figure imgf000347_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(2-oxopyridin-l(2H)-yl)benzyl)isonicotinamide (45 mg, 27% yields for 2 steps) was prepared as described for 2-(3-(lH-pyrazol-l -yl)benzyl)-N-((3-chloro-6-fluoro- lH-indol-5-yl)methyl)isonicotinamide as a white solid. LRMS (M+H+) m/z calculated 487.1, found 487.1. ¾ NMR (CD3OD, 400 MHz) δ 8.62 (d, 1 H), 7.74 (s, 1 H), 7.66-7.42 (m, 6 H), 7.35 (s, 1 H), 7.26 (d, 1 H), 7.25 (s, 1 H), 7.14 (d, 1 H), 6.61 (d, 1H ), 6.46 (t, 1 H), 4.71 (s, 2 H), 4.29 (s, 2 H).
Example 319: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3- morpholinobenzyl)isonicotinamide
Figure imgf000347_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-morpholinobenzyl)isonicotinamide (21mg, 12%> yields for 2 steps) was prepared as described for N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(2-oxopyridin- l(2H)-yl)benzyl)isonicotinamide. LRMS (M+H+) m/z calculated 479.2, found 479.2. ¾ NMR (CD3OD, 400 MHz) δ 8.47 (d, 1 H), 7.56 (s, 1 H), 7.50 (dd, 1 H), 7.41 (d, 1 H), 7.13 (s, 1 H), 7.06 (t, 1 H), 6.78 (d, 1 H),
6.71 (d, 1 H), 6.66 (d, 1 H), 4.58 (s, 2 H), 4.04 (s, 2 H), 3.68 (t, 4 H), 2.98 (t, 4 H).
Example 320: Preparation of N-(4-((4-(((3-chloro-6-fluoro-lH-indol-5-yl)methyl)carbamoyl)pyridin-2- yl)methyl)benzyl)picolinamide
Figure imgf000348_0001
To a suspension of 2-(4-aminomethyl-benzyl)-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-isonicotinamide (100 mg, 0.20 mmol, 1.0 eq) in THF (4 mL) was added pyridine-2-carbonyl chloride (54 mg, 0.30 mmol, 1.5 eq) and Et3N (140 mg, 1.01 mmol, 5 eq). The mixture was stirred at rt for 3 h and diluted with water. The mixture was extracted with DCM. The combined extracts were dried and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/MeOH = 40/1, v/v) to give N-(4-((4-(((3- chloro-6-fluoro-lH-indol-5-yl)methyl) carbamoyl)pyridin-2-yl)methyl)benzyl)picolinamide (45 mg, 42% ) as a white solid. LRMS (M+H+) m/z calculated 528.2, found 528.2. ¾ NMR (CD3OD, 400 MHz) δ 8.62 (t, 1 H), 8.59 (d, 1 H), 8.12 (d, 1 H), 7.96 (t, 1 H), 7.68 (s, 1 H), 7.63 (dd, 1 H), 7.55 (t, 1 H), 7.52 (d, 1 H), 7.30-7.25 (m, 4 H), 7.25 (s, 1 H), 7.14 (d, 1 H), 4.70 (s, 2 H), 4.59 (s, 2 H), 4.20 (s, 2 H).
Example 321, 322, 323, 324, 325, 326, 327 were prepared as described for N-(4-((4-(((3-chloro-6-fluoro-lH- indol-5-yl)methyl)carbamoyl)pyridin-2-yl)methyl)benzyl)picolinamide.
Example 328: Preparation of 2-(amino(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)methyl)-N-((3-chloro-6- fluoro-lH-indol-5-yl)methyl)isonicotinamide.
Figure imgf000348_0002
To a solution of 4-bromo-pyridine-2-carboxylic acid (5.0 g, 24.8 mmol, 1.0 eq) in DMF (10 mL) was added Et3N (7.5 g, 74.4 mmol, 3.0 eq), Ο,Ν-dimethyl-hydroxylamine (2.41 g, 24.8 mmol, 1.0 eq) and HATU (11.3 g, 29.8 mmol, 1.2 eq). The reaction mixture was stirred at rt for 2 h. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and
concentrated to give the crude product which was subject to silica gel chromatography (PE/EA = 5/1-2/1, v/v) to afford 4-bromo-pyridine-2-carboxyl .0 g, 100%) as a white solid.
Figure imgf000348_0003
To a solution of 4-bromo-pyridine-2-carboxylic acid methoxy-methyl-amide (4.0 g, 16.3 mmol, 1.0 eq) in THF (40 mL) was added 4-methylphenyl-magnesium bromide ( 1.0 N, 18 mmol, 1.1 eq). The reaction mixture was stirred at rt for 0.5 h. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated to give the crude product which was subject to silica gel chromatography (PE/EA = 10/1-5/1, v/v) to afford (4-bromo-pyridin-2-yl)-p-tolyl-methanone (3.66 g, 82%) as a white solid.
Figure imgf000349_0001
To a solution of (4-bromo-pyridin-2-yl)-p-tolyl-methanone (3.66 g, 13.3 mmol, 1.0 eq) in MeOH (40 mL) was added Pd(dppf)Ci2 (0.55 g, 0.67 mmol, 0.05 eq), Et3N (2.69 g, 26.6 mmol, 2.0 eq). The reaction mixture was stirred at 100 °C for 12 h under CO (4 MPa). Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated to give the crude product which was subject to silica gel chromatography (PE/EA = 5/1-2/1, v/v) to afford 2-(4-methyl- benzoyl)-isonicotinic acid methyl ester solid.
Figure imgf000349_0002
2-(4-Bromomethyl-benzoyl)-isonicotinic acid methyl ester (3.0 g, 76%>) was prepared as described for 1- bromo-4-bromomethyl-5-chloro-2-
Figure imgf000349_0003
2-[4-(2-Oxo-2H-pyridin-l -ylmethyl)-benzoyl]-isonicotinic acid methyl ester (1.7 g, 54%>) was prepared as described for 3-fluoro-4-((2-oxopyri solid.
Figure imgf000349_0004
2-[4-(2-Oxo-2h-pyridin-l -ylmethyl)-benzoyl]-isonicotinic acid (400 mg, 100%) was prepared as described for l-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6- oxo-l,6-dihydropyridine-3-carboxylic acid as a yellow oil.
Figure imgf000349_0005
N-(3 -chloro-6-fluoro- 1 h-indol-5-ylmeA
(450 mg, 77%) was prepared as described for (5-{5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]- pyridin-3-ylmethyl}-pyridin-2-ylmethyl)-carbamic acid fert-butyl ester as a white solid.
Figure imgf000350_0001
To a solution of N-(3-chloro-6-fluoro-lh-indol-5-ylmethyl)-2-[4-(2-oxo-2H-pyridin-l-ylmethyl)-benzoyl]- isonicotinamide (100 mg, 0.19 mmol, 1.0 eq) in NH3/MeOH (10 mL) was added Ti(i-OPr)4 (110 mg, 0.38 mmol, 2.0 eq) and NaBH4 (15 mg, 0.38 mmol, 2.0 eq). The reaction mixture was stirred for 12 h at rt. Water was added, and the mixture was extracted with EtOAc. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by prep-HPLC to give 2-(amino(4-((2- oxopyridin-l(2H)-yl)methyl)phenyl)methyl) -N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)isonicotinamide (50 mg, 50%) as a white solid. LRMS (M+H+) m/z calculated 516.2, found 516.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.40 (s, 1 H), 9.22 (t, 1 H), 8.58 (d, 1 H), 7.94 (s, 1 H), 7.73 (d, 1 H), 7.60 (d, 1 H), 7.51 (s, 1 H), 7.34-7.41 (m, 3 H), 7.18-7.24 (m, 3 H), 7.15 (d, 1 H), 6.38 (d, 1 H), 6.21 (t, 1 H), 5.15 (s, 1 H), 5.02 (s, 2 H), 4.59 (d, 2 H).
Example 329: Preparation of N-(3-chloro-6-fluoro-lh-indol-5-ylmethyl)-2- {methylamino-[4-(2-oxo-2H- pyridin-l -ylmethyl) -phenyl] -methyl} -isonic
Figure imgf000350_0002
N-(3-chloro-6-fluoro-lh-indol-5-ylmethyl)-2- {methylamino-[4-(2-oxo-2H-pyridin-l -ylmethyl)-phenyl]- methyl} -isonicotinamide (36 mg, 30%>) was prepared as described for 2-(amino(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)isonicotinamide as a white solid.
LRMS (M+H+) m/z calculated 530.2, found 530.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.23 (s, 1 H), 9.22 (t, 1 H), 8.58 (s, 1 H), 7.94 (s, 1 H), 7.92 (s, 1 H), 7.74 (s, 1 H), 7.72 (s, 1 H), 7.34-7.51 (m, 5 H), 7.18-7.24 (m, 3 H), 6.37 (d, 1 H), 6.20 (s, 1 H), 5.15 (s, 1 H), 5.02 (s, 2 H), 4.79 (s, 1 H), 4.59 (s, 2 H), 2.22 (s, 3 H).
Example 330: Preparation of
4-(2-oxo-2H-pyridin- 1 -ylmethyl)-benzyl] - 1 ,4-dihydro-pyridine-3 -carboxylic acid (3 -chloro-6-fluoro- 1 H- indol-5 -ylmethyl) -amide
Figure imgf000351_0001
4-(2-Oxo-2h-pyridin-l -ylmethyl)-benzyl]-l,4-dihydro-pyridine-3-carboxylic acid (3-chloro-6-fluoro-lH- indol-5-ylmethyl)-amide (80 mg, 27.2%) was prepared as described for (5- {5-[(3-chloro-6-fluoro-lH-indol-5- ylmethyl)-carbamoyl]-pyridin-3-ylmethyl}-pyridin-2-ylmethyl)-carbamic acid fert-butyl ester as a white solid. LRMS (M+H+) m/z calculated 517.1, found 517.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 10.62 (t, 1 H), 8.64 (t, 1 H), 7.91 (d, 1 H), 7.76 (d, 1 H), 7.50 (s, 1 H), 7.39-7.43 (m, 2 H), 7.28-7.34 (m, 4 H), 7.22 (d, 1 H), 6.39-6.44 (m, 2 H), 6.20-6.24 (m, 1 H), 5.26 (s, 2 H), 5.08 (s, 2 H), 4.60 (d, 2 H).
Example 331: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-4-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)picolinamide
Figure imgf000351_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-4- (110 mg, 55% yields for 2 steps) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5- oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl] -nicotinamide as a white solid. LRMS (M+H+) m/z calculated 501.1, found 501.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.36 (s, 1 H), 9.18 (t, 1 H), 8.54 (d, 1 H), 7.88 (s, 1 H), 7.75 (dd, 1 H), 7.48 (d, 1 H), 7.46 (d, 1 H), 7.42 (d, 1 H), 7.39 (d, 1 H), 7.26-7.18 (m, 5 H), 6.40 (d, 1 H), 6.21 (t, 1 H), 5.05 (s, 2 H), 4.60 (d, 2 H), 4.05 (s, 2 H).
Example 333: Preparation of 2-(4-(acetamidomethyl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide
Figure imgf000351_0003
2-(4-(Acetamidomethyl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)isonicotinamide (63 mg, 43%>) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2- yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 483.1, found 483.1. ¾ NMR (DMSO- d6, 400 MHz) δ 11.56 (s, 1 H), 9.34 (s, 1 H), 8.58-8.60 (m, 1 H), 8.42 (d, 1 H), 7.66 (d, 2 H), 7.42-7.7.49 (m, 2 H), 7.21-7.29 (m, 2 H), 7.02 (d, 2 H), 4.57 (d, 2 H), 4.21 (d, 2 H), 4.14 (d, 2 H), 1.85 (s, 3 H).
Example 334: Preparation of 2-([2,4'-bipyridin]-6-ylmethyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide
Figure imgf000352_0001
To a solution of N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-(6-hydroxy- pyridin-2-ylmethyl)- isonicotinamide (98 mg, 0.24 mmol, 1 eq) in DCM (5 mL) was added Tf20 (82 mg, 0.29 mmol, 1.5 eq) and Et3N (50 mg, 0.48 mmol, 2 eq) at 0 °C. The mixture was stirred at rt for 5 h and concentrated. The residue was purified by flash chromatography on a silica gel column (DCM/MeOH = 20/1, v/v) to give trifluoro- methanesulfonic acid 6- {4-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-2-ylmethyl}- pyridin-2-yl ester (91 mg, 70%) as a white solid.
Figure imgf000352_0002
To a solution of trifluoro-methanesulfonic acid 6- {4-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]- pyridin-2-ylmethyl} -pyridin-2-yl ester (91 mg, 0.17 mmol, 1 eq) in dioxane (4 mL) and water (1 mL) was added pyridine-4-boronic acid (25 mg, 0.20 mmol, 1.2 eq), Pd(PPh3)4 (23 mg, 0.02 mmol, 0.1 eq), and Na2C03 (54 mg, 0.51 mmol, 3 eq) . The mixture was stirred at 90 °C for 2 h under nitrogen atmosphere. After cooling to rt the solvent was removed in vacuo. The residue was purified by prep-HPLC to afford 2-([2,4'-bipyridin]- 6-ylmethyl)-N-((3-chloro-6-fluoro-lH -indol-5-yl)methyl)isonicotinamide (10 mg, 13%) as an off-white solid. LRMS (M+H+) m/z calculated 472.1, found 472.1. ¾ NMR (CD3OD, 400 MHz) δ 8.51 (d, 1 H), 8.47 (d, 1 H), 8.46 (d, 1 H), 7.92 (d, 1 H), 7.91 (d, 1 H), 7.76 (s, 2 H), 7.75 (d, 1 H), 7.56 (dd, 1 H), 7.41 (d, 1 H), 7.30 (t, 1 H), 7.12 (s, 1 H), 7.01 (d, 1 H), 4.60 (s, 2 H), 4.37 (s, 2 H).
Example 335: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-4-(4-((2-oxopyridin -1(2H)- yl)methyl)benzyl)-l ,6-dihydropyridine-2-carboxamide
Figure imgf000352_0003
To a mixture of methyl 4-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)picolinate (500 mg, 1.5 mmol, 1.0 eq) in EA/water (10 mL/0.5 mL) was added CO(NH2)2.H202 (422 mg, 4.5 mmol, 3.0 eq) followed by phthalic anhydrde (666 mg, 4.5 mmol, 3.0 eq) portionwise at 0 °C. The resultant mixture was heated to 45 °C and kept stirring for 4 h. The reaction mixture was quenched with 10% of Na2S04 (1.5 eq), and extracted with DCM. The organic layer was washed with sat.Na2CC>3, followed by brine, dried over Na2SC>4, filtered, and concentrated. The residue was purified by column chromatography (DCM-DCM/MeOH = 20/1, v/v) to give 2-(methoxycarbonyl)-4-(4-((2-oxopyridin-l(2H)-yl) methyl)benzyl)pyridine 1-oxide (100 mg, 19%) as a yellow solid. LRMS (M+H+) m/z calculated 351, found 351.
Figure imgf000353_0001
The mixture of 2-(methoxycarbonyl)-4-(4-((2-oxopyridin-l(2H)-yl) methyl)benzyl)pyridine 1-oxide (300 mg, 0.86 mmol, 1.0 eq) in Ac20 (5 mL) was kept stirring for 1 h at 130 °C. And then the solvent was removed. The residue was dissolved in DCM (30 mL), washed with sat.NaHCCb. The organic layer was dried over Na2SC>4, filtered, and concentrated. The residue was dissolved in MeOH (5 mL). To the mixture was added K2C03 (237 mg), and the reaction mixture was kept stirring for 3 h. The reaction mixture was concentrated, and the residue was purified by prep-HPLC to give methyl 6-oxo-4-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)-l,6-dihydropyridine-2-carboxylate (150 mg, 50%>) as a yellow solid. LRMS (M+H+) m/z calculated 351, found 351.
Figure imgf000353_0002
6-Oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl) -l ,6-dihydropyridine-2-carboxylic acid (200 was prepared as described for l-(3-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6- dihydropyridine-3-carboxylic +) m/z calculated 337, found 337.
Figure imgf000353_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-l,6- dihydropyridine-2-carboxamide (10 mg, 9%>) was prepared as described for (5-{5-[(3-chloro-6-fluoro-lH- indol-5-ylmethyl)-carbamoyl]-pyridin-3-ylmethyl}-pyridin -2-ylmethyl)-carbamic acid tert-butyl ester as an off-white solid. LRMS (M+H+) m/z calculated 517, found 517. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.43 (s, 1 H), 9.21 (t, 1 H), 8.56 (d, 1 H), 8.03 (s, 1 H), 7.75 (d, 1 H), 7.59 (d, 1 H), 7.48 (d, 1 H), 7.39-7.41 (m, 2 H), 7.35-7.38 (m, 2 H), 7.19-7.26 (m, 3 H), 6.38 (d, 1 H), 6.25 (d, 1 H), 6.20 (t, 1 H), 5.80 (d, 1 H), 5.06 (s, 2 H), 4.59 (d, 2 H).
Example 336: Preparation of 2-([l, -biphenyl]-4-ylmethyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl) methyl)isonicotinamide
Figure imgf000354_0001
2-([l,l'-Biphenyl]-4-ylmethyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl) isonicotinamide (47 mg, 30%) was prepared as described for (5- {5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-3- ylmethyl}-pyridin-2-ylmethyl)-carbamic acid fert-butyl ester as an off- white solid. LRMS (M+H+) m/z calculated 470, found 470. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.40 (s, 1 H), 9.25 (t, 1 H), 8.64 (d, 1 H), 7.56 (s, 1 H), 7.57-7.65 (m, 5 H), 7.50 (d, 1 H), 7.42-7.46 (m, 3 H), 7.32-7.38 (m, 3 H), 7.22 (d, 1 H), 4.59 (d, 2 H), 4.19 (s, 2 H).
Example 337: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-phenylthiazol-2- yl)methyl)isonicotinamide
Figure imgf000354_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-phenylthiazol-2-yl)methyl)isonicotinamide (40 mg,
37.2%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin-2- yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 477.1, found 477.1. ¾NMR (DMSO-<¾, 300 MHz) δ 11.42 (s, 1 H), 9.31 (t, 1 H), 8.71 (d, 1 H), 7.99 (s, 1 H), 9.30 (d, 3 H), 7.73-7.75 (m, 1 H), 7.22- 7.52 (m, 6 H), 4.62 (d, 4 H).
Example 338: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)pyridazine-4-carboxamide
Figure imgf000354_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridazine-4- carboxamide (18 mg, 22.36%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6- (5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl] -nicotinamide. LRMS (M+H+) m z calculated 501.7, found 501.7.
Ή NMR (CD3OD, 400 MHz) δ 9.25 (s, 1 H), 7.75 (s, 1 H), 7.51 (d, 1 H), 7.35-7.39 (m, 2 H), 7.12 (t, 5 H), 6.99 (d, 1 H), 6.41 (d, 1 H), 6.22 (t, 1 H), 5.00 (s, 2 H), 4.55 (s, 2 H), 4.21 (s, 2 H).
Example 339: Preparation ofN-(3-Chloro-6-fluoro-lH-indol-5-ylmethyl)-2-{4-[(2-hydroxy-acetylamino)- methyl] -3 -methoxy-benzyl } -isonicotinamide
Figure imgf000355_0001
(4- {4-[(3-Chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-2-ylmethyl} -2-methoxy-benzyl)- carbamic acid fert-butyl ester (0.54 mg, 75%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5- ylmethyl)-5-[6-(5-oxo-pyrroli
Figure imgf000355_0002
The mixture of (4- {4-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]-pyridin-2 -ylmethyl} -2-methoxy- benzyl)-carbamic acid tert-butyl ester (0.54 g, 1 mmol) with HC1/EA was stirred at rt for 3 h. Then it was concentrated in vacuum to get 2-(4-aminomethyl-3-methoxy-benzyl)-N-(3-chloro-6-fluoro-lH-indol-5- ylmethyl)-isonicotinamide hydro
Figure imgf000355_0003
To a solution of compound 2-(4-aminomethyl-3-methoxy-benzyl)-N-(3-chloro-6- fluoro-lH-indol-5- ylmethyl)-isonicotinamide hydrochloride salt (0.15 g, 0.2 mmol, 1 eq) in DMF (5 mL) was added hydroxy- acetic acid (0.023 g, 0.3 mmol, 1.5 eq), HOBT (0.033 g, 0.25 mmol, 1.2 eq), EDCI (0.048 g, 0.25 mmol, 1.2 eq) and Et3N (0.083 g, 0.82 mmol, 4 eq). The mixture was stirred at rt overnight. The residue was purified by chromatography on a silica gel column (DCM/MeOH = 20/1, v/v) to give N-(3-chloro-6-fluoro-lH-indol-5- ylmethyl)-2- {4- [(2-hydroxy-acetylamino)-methyl]-3-methoxy-benzyl} -isonicotinamide (25 mg, 16%>) as a white solid. LRMS (M+H+) m/z calculated 510.1, found 510.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.50 (s, 1 H), 9.40 (t, 1 H), 8.70 (s, 1 H), 8.60 (s, 1 H), 8.04 (s, 3 H), 7.86 (d, 2 H), 7.51 (d, 1 H), 7.46 (d, 1 H), 7.24 (d, 1 H), 7.15 (d, 1 H), 7.04(s, 1 H), 6.88(d, 1 H), 4.58 (d, 2 H), 4.24 (s, 4 H), 3.89 (s, 4 H), 3.56 (m, 3 H).
Example 340: Preparation of 2-(4-(acetamidomethyl)-3-methoxybenzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide
Figure imgf000355_0004
2-[4-(Acetylamino-methyl)-3-methoxy-benzyl]-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-isonicotinamide (7 mg, 7%>) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2- {4-[(2-hydroxy- acetylamino)-methyl] -3 -methoxy-benzyl} -isonicotinamide. LRMS (M+H+) m/z calculated 495.2, found 495.1. 'H NMR (DMSO-ifc, 400 MHz) δ 11.50 (s, 1 H), 9.20 (t, 1 H), 8.70 (d, 1 H), 8.10 (s, 1 H), 7.69 (s, 1 H), 7.63 (t, 1H), 7.51 (d, 1 H), 7.45 (d, 1 H), 7.24 (d, 1 H), 7.05 (d, 1 H), 6.92 (s, 1 H), 6.81 (d, 1 H), 4.58 (d, 2 H), 4.15 (s, 4 H), 3.75 (s, 3 H), 1.83 (s, 3 H). Example 341: Preparation of 2-{4-[(Acetyl-cyclopropyl-amino)-methyl]-benzyl}-N-(3-chloro-4-fluoro-lH- indol-5-ylmethyl)-isonicotinamide
Figure imgf000356_0001
N-({4-[(4- {N-[(3-chloro-4-fluoroindol-5-yl)methyl]carbamoyl}(2-pyridyl))methyl]phenyl}methyl)-N- cyclopropylacetamide (11 mg, 7%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)- 5-[6-(5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 505.2, found 505.2. 'H NMR (DMSO-ifc, 400 MHz) δ 11.58 (s, 1 H), 9.22 (t, 1 H), 8.62(d, 1 H), 7.73 (s, 1 H), 7.65 (m, 1 H), 7.50 (d, 1H), 7.22 (m, 3 H), 7.12 (m, 3 H), 4.56 (d, 2 H), 4.43 (s, 2 H), 4.12 (s, 2 H), 2.61(t, 1 H), 2.15 (d, 3 H), 0.75 (m, 3 H). Example 342: Preparation of 2-{4-[(acetyl-cyclopropyl-amino)-methyl]-benzyl}-N-(3-chloro-6-fluoro-lH- indol-5-ylmethyl)-isonicotinamide
Figure imgf000356_0002
2-{4-[(Acetyl-cyclopropyl-amino)-methyl]-benzyl}-N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)- isonicotinamide (27 mg, 18%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6- (5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl] -nicotinamide. LRMS (M+H+) m/z calculated 505.2, found 505.2. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.58 (s, 1 H), 9.22 (t, 1 H), 8.62(d, 1 H), 7.75 (s, 1 H), 7.67(m, 1 H), 7.50 (d, 1H), 7.45 (m, 1 H), 7.23 (m, 3 H), 7.12 (m, 2 H), 4.56 (d, 2 H), 4.43 (s, 2 H), 4.12 (s, 2 H), 2.61 (t, 1 H), 2.15 (d, 3 H), 0.75 (m, 4 H). Example 343: Preparation ofN-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-2-{6-[(2-hydroxy-acetylamino)- methyl]-pyridin-3-ylmethyl} -isonicotinamide
Figure imgf000356_0003
N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5- {6-[(2-h^
nicotinamide (20 mg, 35.3%) was prepared as described for N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-5-[6- (5-oxo-pyrrolidin-2-yl)-pyridin-3-ylmethyl]-nicotinamide as yellow oil. LCMS (M+H+) m/z calculated 482.1 , found 482.1. ¾ NMR (CD3OD, 400 MHz) δ 8.61 (d, 1 H), 8.46 (d, 1 H), 7.74-7.71 (m, 2 H), 7.65 (d, I H), 7.54 (d, 1 H), 7.32 (d, 1 H), 7.25 (s, 1 H), 7.14 (d, 1 H), 4.72 (s, 2 H), 4.54 (s, 2 H), 4.24 (s, 2 H), 4.05 (d, 2 H).
Example 344: Preparation of 2-((6-((2-aminoacetamido)methyl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro- 1 H-indol-5 -yl)methyl)isonicotinamide
Figure imgf000357_0001
The mixture of {[(5- {5-[(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-carbamoyl]- pyridin-3-ylmethyl} -pyridin-2 ylmethyl)-carbamoyl] -methyl} -carbamic acid tert-butyl ester (80 mg, 0.14 mmol, 1.0 eq) in DCM (10 mL) was added HCl (4 N in dioxane, 3 mL). The mixture was stirred at rt for 1 h. The mixture was evaporated in vacuum to residue, which was purified by pre-HPLC (MeCN/H20 from 5/100 to 95/100, v/v) to give of 5- {6- [(2-amino-acetylamino)-methyl]-pyridin-3-ylmethyl} -N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)- nicotinamide (40 mg, 59.5 %) as yellow solid. LRMS (M+H+) m/z calculated 481.1 , found 481.1. ¾ NMR (Methanol-^, 400 MHz) δ 8.91 -8.88 (m, 2 H), 8.60 (d, 1 H), 8.16-8.14 (m, 2 H ), 8.05 (d, 1 H), 7.56 (d, 1 H), 7.27 (s, 1 H), 7.16 (d, 1 H), 4.76 (s, 2 H), 4.71 (s, 2 H), 3.89 (s, 2 H), 3.37 (s, 2 H). Example 345: Preparation of 2-(4-((2-aminoacetamido)methyl)-3-methoxybenzyl)-N-((3-chloro-6-fluoro-lH- indol-5-yl)methyl)isonicotinamide
Figure imgf000357_0002
2-(4-((2-Aminoacetamido)methyl)-3-methoxybenzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide hydrochloride (30 mg, 29%) was prepared as described for 5- {6-[(2-amino- acetylamino)-methyl]-pyridin-3-ylmethyl} -N-(3-chloro-6-fluoro-lH-indol-5-ylmethyl)-nicotinamide. LRMS (M+H+) m/z calculated 510.2, found 510.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 1 1.50 (s, 1 H), 9.20 (t, 1 H), 8.70 (d, 1 H), 8.10 (s, 1 H), 7.91 (t, 1 H), 7.69 (s, 1 H), 7.63 (t, 1H), 7.51 (s, 1 H), 7.45 (d, 1 H), 7.24 (d, 1 H), 7.05 (d, 1 H), 6.92 (s, 1 H), 6.81 (d, 1 H), 5.48 (t, 1 H), 4.58 (d, 2 H), 4.23 (d, 2 H), 4.12 (d, 2 H), 3.83 (d, 2 H), 3.77 (s, 3 H). Example 346: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4- phenoxybenzyl)isonicotinamide
Figure imgf000358_0001
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-phenoxybenzyl)isonicotinamide (25 mg, 41% yields for 2 steps) was prepared as described for 5- {6-[(2-amino-acetylamino)-methyl]-pyridin-3-ylmethyl}-N-(3-chloro- 6-fluoro-lH-indol-5-ylmethyl)-nicotinamide as a white solid. LRMS (M+H+) m/z calculated 486.1, found 486.1. 'H NMR (DMSO-ifc, 400 MHz) δ 11.39 (s, 1 H), 9.24 (t, 1 H), 8.64 (d, 1 H), 7.72 (s, 1 H), 7.64 (d, 1 H), 7.50-7.21 (m, 7 H), 7.70-6.91 (m, 5 H), 4.60 (d, 1 H), 4.12 (s, 1 H)
Example 347: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyridin-3- yloxy)benzyl)isonicotinamide
Figure imgf000358_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyridin-3-yloxy)benzyl)isonicotinamide (37 mg, 20%> yields for 2 steps) was prepared as described for N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4- phenoxybenzyl)isonicotinamide as a white solid. LRMS (M+H+) m/z calculated 487.1, found 487.1. ¾ NMR (CDCls, 400 MHz) δ 8.66-8.64 (d, 1 H), 8.41-8.33 (m, 3 H), 7.62-7.60 (d, 1 H), 7.50 (s, 1 H), 7.43-7.41 (d, 1 H), 7.28-7.23 (m, 3 H), 7.17-7.16 (d, 1 H), 7.08-7.05 (d, 1 H), 6.95-6.93 (d, 2 H), 6.52 (s, 1 H), 4.77-4.76 (d, 2 H), 4.18 (s, 2 H)
Example 348: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l-((3-((2-oxopyridin-l(2H)- yl)methyl)bicyclo [1.1.1 Jpentan- 1 -yl)methyl)- 1 H-pyrazole-4-carboxamide
Figure imgf000358_0003
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l -((3-((2-oxopyridin-l(2H)-yl)methyl)bicyclo[l .l . l]pentan-l- yl)methyl)-lH-pyrazole-4-carboxamide (55 mg, 42%>) was prepared as described for N-(3-chloro-6-fluoro- lH-indol-5-ylmethyl)-5-[6-(5-oxo-pyrrolidin -2-yl)-pyridin-3-ylmethyl]-nicotinamide. LRMS (M+H+) m/z calculated 480.1, found 480.1. ¾ NMR (DMSO-ifc, 400 MHz) δ 11.37 (s, 1 H), 8.53 (t, 1 H), 8.09 (s, 1 H), 7.85 (s, 1 H), 7.50-7.47 (m, 2 H), 7.43-7.41 (m, 1 H), 7.32-7.38 (m, 1 H), 7.22-7.20 (d, 1 H), 6.35-6.32 (d, 1 H), 6.15-6.14 (t, 1 H), 4.51-4.50 (d, 2 H), 4.19 (s, 2 H), 3.97 (s, 2 H), 1.47 (s, 6 H).
Example 349: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide
Figure imgf000359_0001
To a solution of 2-(3-methyl-quinolin-6-ylmethyl)-isonicotinic acid (150 mg, crude) in DMF (15 mL) was added 6-Aminomethyl-isoquinolin-l -ylamine (62 mg, 0.36 mmol, 1.0 eq) followed by EDCI (104 mg, 0.54 mmol, 1.5 eq), HOBT (73 mg, 0.54 mmol, 1.5 eq) and TEA (109 mg, 1.08 mmol, 3.0 eq). The reaction mixture was heated to 40°C kept stirring for overnight. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2S04, filtered, and concentrated. The residue was purified by prep-HPLC to give N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl) isonicotinamide (30 mg, 19%) as a white solid. LRMS (M+H+) m/z calculated 434, found 434. ¾ NMR (DMSO-ifc, 400 MHz) δ 9.40 (t, 1 H), 8.71 (s, 1 H), 8.67 (d, 1 H), 8.13 (d, 1 H), 8.07 (s, 1 H), 7.90 (d, 1 H), 7.75-7.80 (m, 3 H),7.68 (d, 1 H), 7.62 (d, 1 H), 7.54 (s, 1 H), 7.49 (d, 1 H), 6.84 (d, 1 H), 6.77 (s, 2 H), 4.60 (d, 2 H), 4.35 (s, 2 H), 2.46 (s, 3 H).
Example 350: Preparation of N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide
Figure imgf000359_0002
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide (30 mg, 18%) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide as a yellow solid. LRMS (M+H+) m/z calculated 459, found 459. 1H NMR (DMSO-i¾, 300 MHz) δ 11.42 (br, 1 H), 9.25 (t, 1 H), 8.71 (s, 1 H), 8.65 (d, 1 H), 8.05 (s, 1 H), 7.89 (d, 1 H), 7.76 (d, 2 H), 7.60-7.66 (m, 2 H),7.51 (d, 1 H), 7.43 (d, 1 H), 7.22 (d, 2 H), 4.58 (d, 2 H), 4.34 (s, 2 H), 2.46 (s, 3 H).
Example 351: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide
Figure imgf000360_0001
To a solution of 2-(3-methyl-quinolin-6-ylmethyl)-isonicotinic acid (150 mg, crude) in DMF (15 mL) was added 5-aminomethyl-4,6-dimethyl-pyridin-2-ylamine dihydrochloride (400 mg, crude) followed by EDCI (104 mg, 0.54 mmol, 1.5 eq), HOBT (73 mg, 0.54 mmol, 1.5 eq) and TEA (109 mg, 1.08 mmol, 3.0 eq). The reaction mixture was heated to 40 °C kept stirring for overnight. Water was added, and the mixture was extracted with DCM. The organic layer was washed with water, dried over Na2SO i, filtered, and concentrated. The residue was purified by prep-HPLC to give N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3- methylquinolin-6-yl)methyl)isonicotinamide (25 mg, 17%) as a yellow solid. LRMS (M+H+) m/z calculated 412, found 412. ¾ NMR (DMSO-ifc, 300 MHz) δ 8.71 (s, 1 H), 8.65 (t, 1 H), 8.60 (d, 1 H), 8.06 (s, 1 H), 7.89 (d, 1 H), 7.74 (d, 2 H),7.60 (d, 2 H), 6.10 (s, 1 H), 5.70 (s, 2 H), 4.32 (s, 4 H), 2.46 (s, 3 H), 2.29 (s, 3 H), 2.15 (s, 3 H).
Example 352: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methylisoquinolin-6- yl)methyl)isonicotinamide
Figure imgf000360_0002
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methylisoquinolin-6-yl)methyl)isonicotinamide (29 mg, 21% yields for 2 steps) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-((3- methylquinolin-6-yl)methyl)isonicotinamide. LRMS (M+H+) m/z calculated 412.2, found 412.2. ¾ NMR (CD3OD, 400 MHz) δ 9.05 (s, 1 H), 8.61 (d, 1 H), 7.96 (s, 1 H), 7.73 (s, 1 H), 7.69 (s, 1 H), 7.62 (dd, 1 H), 7.55 (s, 1 H), 7.52 (d, 1 H), 6.30 (s, 1 H), 4.49 (s, 2 H), 4.38 (s, 2 H), 2.64 (s, 3 H), 2.39 (s, 3 H), 2.26 (s, 3 H).
Example 353: Preparation of N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-(aminomethyl)quinolin-6- yl)methyl)isonicotinamide
Figure imgf000360_0003
(6- {4-[(l-Amino-isoquinolin-6-ylmethyl)-carbamoyl]-pyridin-2-ylmethyl} -quinolin-2-ylmethyl)-carbam acid tert-butyl ester (70 mg, 35%) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-((3- methylquinolin-6-yl)methyl)isonicotinamide.
Figure imgf000361_0001
To a solution of (6- {4-[(l -amino-isoquinolin-6-ylmethyl)-carbamoyl]-pyridin-2-ylmethyl} -quinolin-2- ylmethyl)-carbamic acid tert-butyl ester (80 mg, 0.13 mmol) in EA (1 mL) was added HCl/EA solution. The mixture was stirred at rt for 1 h. The precipitate was collected by filtration to give N-((l-aminoisoquinolin-6- yl)methyl)-2-((2-(aminomethyl)quinolin-6-yl)methyl) isonicotinamide (50 mg, 66%) as an off-white solid. LRMS (M+H+) m/z calculated 449.2, found 449.2. ¾ NMR (DMSO-ifc, 400 MHz) 8 13.51 (s, 1 H), 10.05 (s, 1 H), 9.25 (br, 1 H), 8.88 (d, 1 H), 8.63-8.62 (m, 4 H), 8.45 (d, 1 H), 8.19 (s, 1 H), 8.09-8.01 (m, 3 H), 7.89 (d, 1 H), 7.85 (s, 1 H), 7.73-7.64 (m, 4 H), 7.20 (d, 1 H), 4.69 (d, 2 H), 4.59 (s, 2 H), 4.39 (t, 2 H).
Example 354: Preparation of 2-((2-(aminomethyl)quinolin-6-yl)methyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide
Figure imgf000361_0002
2-((2-(Aminomethyl)quinolin-6-yl)methyl)-N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)isonicotinamide (60 mg, 79%>) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-(aminomethyl)quinolin- 6-yl)methyl)isonicotinamide. LRMS (M+H+) m/z calculated 474.1, found 474.1. ¾ NMR (DMSO-tfc, 400 MHz) δ 11.53 (s, 1 H), 9.58 (s, 1 H), 8.83 (d, 1 H), 8.60 (br, 1 H), 8.43 (d, 1 H), 8.10 (d, 1 H), 8.02-8.00 (m, 3 H), 7.85 (d, 1 H), 7.62 (d, 1 H), 7.51 (s, 1 H), 7.47 (d, 1 H), 7.44 (d, 1 H), 7.31 (s, 1 H), 7.24 (d, 1 H), 7.20 (d, 1 H), 4.60 (d, 2 H), 4.57 (s, 2 H), 4.40 (q, 2 H).
Example 355: Preparation of N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-(aminomethyl)quinolin-6- yl)methyl)isonicotinamide
Figure imgf000361_0003
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-(aminomethyl)quinolin-6-yl)methyl)isonicotinamide (35 mg, 53%o) was prepared as described for N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-(aminomethyl)quinolin- 6-yl)methyl)isonicotinamide. LRMS (M+H+) m/z calculated 427.2, found 427.2. ¾ NMR (CD3OD, 400 MHz) δ 8.99 (d, 1 H), 8.69 (d, 1 H), 8.39 (s, 1 H), 8.35 (d, 1 H), 8.25 (d, 1 H), 8.16 (s, 1 H), 7.98 (d, 1 H), 7.80 (d, 1 H), 6.74 (s, 1 H), 4.82 (s, 2 H), 4.63 (s, 2 H), 4.59 (s, 2 H), 2.63 (s, 3 H), 2.50 (s, 3 H). Example 356:
Illustrative compounds of the present invention are listed in Table 1. Table 2 shows IC50 values for the compounds in Table 1 against human plasma kallikrein. The scale utilized in Table 2 is as follows: +++ less than 100 nM, ++ between 100 and 1000 nM, and + greater than 1000 nM.
Table 1. Illustrative Compounds of the Present Invention
Figure imgf000362_0001
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-
C21
yl)methyl)phenoxy)benzamide
N-((5-chloro-lH-indazol-3-yl)methyl)-6-oxo-l-(4-((2-oxopyridin-l(2H)-
C22
yl)methyl)benzyl)piperidine-3-carboxamide
N-((5-chloro-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin-l(2H)-
C23
yl)methyl)phenyl)sulfonyl)benzamide
C24 N-(4-aminobenzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide
N-((5-chlorobenzo[b]thiophen-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C25
yl)methyl)benzyl)nicotinamide
methyl 2-(5-chloro-lH-indazol-3-yl)-2-(3-(4-((2-oxopyridin-l (2H)-
C26
yl)methyl)benzyl)benzamido)acetate
N-(4-(aminomethyl)benzyl)-5-(4-((2-oxopyridin-l(2H)-
C27
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-
C28
yl)methyl)benzyl)picolinamide
N-((6-aminopyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C29
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(hydroxy(4-((2-oxopyridin-l(2H)-
C30
yl)methyl)phenyl)methyl)benzamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C31
yl)methyl)benzyl)nicotinamide
C32 5-(4-carbamoylbenzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C33
yl)methyl)benzyl)isonicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C34
yl)methyl)phenoxy)nicotinamide
methyl 2-(5-chloro-lH-indazol-3-yl)-2-(5-(4-((2-oxopyridin-l (2H)-
C35
yl)methyl)benzyl)nicotinamido)acetate
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxopyrrolidin-l -
C36
yl)benzyl)nicotinamide
N-((6-chloro-lH-indol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C37
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C38
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-fluoro-4-((2-oxopyridin-l(2H)-
C39
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-chloro-4-((2-oxopyridin-l(2H)-
C40
yl)methyl)benzyl)nicotinamide
N-((5-chloro-l -methyl-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C41
yl)methyl)benzyl)nicotinamide
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(methoxy(4-((2-oxopyridin- 1 (2H)-
C42
yl)methyl)phenyl)methyl)benzamide
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(l -hydroxy-l -(4-((2-oxopyridin-
C43
1 (2H) -yl)methyl)phenyl) ethyl)benzamide
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(difluoro(4-((2-oxopyridin-l(2H)-
C44
yl)methyl)phenyl)methyl)benzamide
N-((lH-indol-6-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C45
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxopyridin-l(2H)-
C46
yl)benzyl)nicotinamide 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(3-(4-((2-oxopyridin-l(2H)-
C47
yl)methyl)benzyl)phenyl)acetic acid
N-(( 1 H-indazol-3 -yl)methyl)-5 -(4-((2-oxopyridin- 1 (2H)-
C48
yl)methyl)benzyl)nicotinamide
N-((lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C49
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-fluoro-4-((2-oxopyridin-l(2H)-
C50
yl)methyl)benzyl)nicotinamide
N-((6-amino-2-methylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C51
yl)methyl)benzyl)nicotinamide
C52 N-(3 -chlorobenzyl)-5-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((3-
C53
oxomorpholino)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-cyano-4-((2-oxopyridin-l(2H)-
C54
yl)methyl)benzyl)nicotinamide
5-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-
C55
indazol-3-yl)methyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2,5-difluoro-4-((2-oxopyridin-l(2H)-
C56
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2,5-dichloro-4-((2-oxopyridin-l(2H)-
C57
yl)methyl)benzyl)nicotinamide
N-((6-chloro-lH-benzo[d]imidazol-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C58
yl)methyl)benzyl)nicotinamide
N-((3-chloro-lH-indol-6-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C59
yl)methyl)benzyl)nicotinamide
N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C60
yl)methyl)benzyl)nicotinamide
N-((5-amino-3-methylpyrazin-2-yl)methyl)-5-(4-((2-oxopyridin-l (2H)-
C61
yl)methyl)benzyl)nicotinamide
N-((5-aminopyridin-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C62
yl)methyl)benzyl)nicotinamide
N-(4-chloro-2-methylbenzyl)-5-(4-((2-oxopyridin-l (2H)-
C63
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-
C64
yl)methyl)pyridin-3-yl)methyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(5-chloro-2-fluoro-4-((2-oxopyridin-
C65
l(2H)-yl)methyl)benzyl)nicotinamide
N-((6-amino-4-methylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C66
yl)methyl)benzyl)nicotinamide
N-((3-aminobenzo[d]isoxazol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C67
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-l-(2,5-difluoro-4-((2-oxopyridin-l(2H)-
C68
yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
N-((5-chloro-lH-indazol-3-yl)methyl)-l-(2,5-dichloro-4-((2-oxopyridin-l(2H)-
C69
yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
N-((5-chloro-lH-indazol-3-yl)methyl)-l-(5-chloro-2-fluoro-4-((2-oxopyridin-
C70
l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide methyl 2-(5-chloro-lH-indazole-3-carboxamido)-2-(5-(4-((2-oxopyridin-l(2H)-
C71
yl)methyl)benzyl)pyridin-3-yl)acetate
2-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-
C72
yl)methyl)nicotinamido)methyl)-lH-indazol-l -yl)acetic acid N-(l -(5-chloro-lH-indazol-3-yl)ethyl)-5-(4-((2-oxopyridin-l(2H)-
C73
yl)methyl)benzyl)nicotinamide
2-(5-chloro-3-((5-(4-((2-oxopyridin-l (2H)-
C74
yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol-l-yl)acetic acid
N-((6-methyl H-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-
C75
l(2H)-yl)methyl)benzyl)nicotinamide
l-(4-((4-(l-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2,2,2-trifluoroethyl)-
C76
1 H-pyrazol- 1 -yl)methyl)benzyl)pyridin-2( 1 H)-one
N-((3-chloro-6-methyl-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-
C77
oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinamide
5-chloro-N-(2,2,2-trifluoro-l -(1 -(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)-
C78
1 H-pyrazol-4-yl)ethyl)- 1 H-indazole-3-carboxamide
N-((l -(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-
C79
oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinamide
N-((l-(3-amino-3-oxopropyl)-5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-
C80
oxopyridin- 1 (2H)-yl)methyl)pyridin-3 -yl)methyl)nicotinamide
3-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-
C81
yl)methyl)nicotinamido)methyl)- 1 H-indazol- 1 -yl)propanoic acid ethyl 3-(5-chloro-3-((5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-
C82
yl)methyl)nicotinamido)methyl)- 1 H-indazol- 1 -yl)propanoate
N-((3-chloro-6-methyl-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C83
yl)methyl)benzyl)nicotinamide
N-((6-methyl-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C84
yl)methyl)benzyl)nicotinamide
N-((l -(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-
C85
oxopyridin- 1 (2H)-yl)methyl)pyridin-3 -yl)methyl)nicotinamide
methyl 2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(5-(4-((2-oxopyridin-
C86
l(2H)-yl)methyl)benzyl)pyridin-3-yl)acetate
2-(((5-chloro-lH-indazol-3-yl)methyl)amino)-2-(5-(4-((2-oxopyridin-l(2H)-
C87
yl)methyl)benzyl)pyridin-3-yl)acetic acid
2-(5-chloro-lH-indazole-3-carboxamido)-2-(5-(4-((2-oxopyridin-l (2H)-
C88
yl)methyl)benzyl)pyridin-3-yl)acetic acid
5-(4-(( 1 H-pyrazol- 1 -yl)methyl)benzyl)-N-((5 -chloro- 1 H-indazol-3 -
C89
yl)methyl)nicotinamide
N-(l -(5-chloro-lH-indazol-3-yl)ethyl)-5-((6-((2-oxopyridin-l(2H)-
C90
yl)methyl)pyridin-3-yl)methyl)nicotinamide
N-((5-chloro-l-(2-hydroxyethyl)-lH-indazol-3-yl)methyl)-5-((6-((2-
C91
oxopyridin- 1 (2H)-yl)methyl)pyridin-3 -yl)methyl)nicotinamide
methyl 2-(5-chloro-3 -((5-((6-((2-oxopyridin- 1 (2H)-yl)methyl)pyridin-3 -
C92
yl)methyl)nicotinamido)methyl)- 1 H-indazol- 1 -yl)acetate
ethyl 3-(5-chloro-3-((5-(4-((2-oxopyridin-l(2H)-
C93
yl)methyl)benzyl)nicotinamido)methyl)-lH-indazol-l-yl)propanoate
3-(5-chloro-3-((5-(4-((2-oxopyridin-l (2H)-
C94
yl)methyl)benzyl)nicotinamido)methyl)- 1 H-indazol- 1 -yl)propanoic acid
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopiperazin-l -
C95
yl)methyl)benzyl)nicotinamide
N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C96
yl)methyl)benzyl)nicotinamide
N-((3 -chloro- 1 H-pyrrolo [2,3 -b]pyridin-5 -yl)methyl)-5 -(4-((2-oxopyridin-
C97
l(2H)-yl)methyl)benzyl)nicotinamide
5-(4-(2-amino-2-oxoethyl)benzyl)-N-((5-chloro-lH-indazol-3-
C98
yl)methyl)nicotinamide 5-((2-(aminomethyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3-
C99
yl)methyl)nicotinamide
N-((3-fluoro-4-methylpyridin-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
CIOO
yl)methyl)benzyl)nicotinamide
methyl 2-(4-((5-(((5-chloro- 1 H-indazol-3 -yl)methyl)carbamoyl)pyridin-3 -
ClOl
yl)methyl)phenyl)acetate
methyl 3 -( 1 H-indazol-3 -yl)-3 -(5 -(4-((2-oxopyridin- 1 (2H)-
C102
yl)methyl)benzyl)nicotinamido)propanoate
5-(4-(acetamidomethyl)benzyl)-N-((3 -chloro- 1 H-indol-5-
C103
yl)methyl)nicotinamide
5-((2-(aminomethyl)pyridin-3-yl)methyl)-N-((3-chloro-lH-indol-5-
C104
yl)methyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyrazin-l(2H)-
C105
yl)methyl)benzyl)nicotinamide
N-(l -(5-chloro-lH-indazol-3-yl)-2-hydroxyethyl)-5-(4-((2-oxopyridin-l(2H)-
C106
yl)methyl)benzyl)nicotinamide
C107 N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-(ureidomethyl)benzyl)nicotinamide
6-((5 -(((3 -chloro- 1 H-indol-5-yl)methyl)carbamoyl)pyridin-3 -
C108
yl)methyl)quinoline-2-carboxamide
N-((6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C109
yl)methyl)benzyl)nicotinamide
Cl lO 5-(2-(aminomethyl)benzyl)-N-((3-chloro-lH-indol-5-yl)methyl)nicotinamide cm methyl 3-(5-chloro-lH-indazol-3-yl)-3-(5-(4-((2-oxopyridin-l (2H)- yl)methyl)benzyl)nicotinamido)propanoate
N-((3-chloro-lH-indol-5-yl)methyl)-5-((2-methoxyquinazolin-6-
CU2
yl)methyl)nicotinamide
N-(2-(guanidinooxy)ethyl)-5-(4-((2-oxopyridin-l(2H)-
C113
yl)methyl)benzyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C114
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-((2-methoxyquinazolin-6-
C115
yl)methyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-(2-oxooxazolidin-3-
C116
yl)benzyl)nicotinamide
methyl 3-(4-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-
C117
yl)methyl)phenyl)propanoate
N-((5 -chloro- 1 H-indazol-3 -yl)methyl)-5 -(4-(2-oxoimidazolidin- 1 -
C118
yl)benzyl)nicotinamide
5-(4-(3 -amino-3 -oxopropyl)benzyl)-N-((3 -chloro- 1 H-indol-5 -
C119
yl)methyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyrrolidin-l-
C120
yl)methyl)benzyl)nicotinamide
methyl 6-((5-(((3-chloro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-
C121
yl)methyl)quinoline-2-carboxylate
5-(4-(2-amino-2-oxoethoxy)benzyl)-N-((3 -chloro- lH-indol-5-
C122
yl)methyl)nicotinamide
N-((3 -chloro- 1 H-indol-5 -yl)methyl)-5 -(4-((N-
C123
methylacetamido)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((N-
C124
methylacetamido)methyl)benzyl)nicotinamide (S)-5-(4-(l-acetamidoethyl)benzyl)-N-((3-chloro-lH-indol-5-
C125
yl)methyl)nicotinamide
(S)-5-(4-(l-acetamidoethyl)benzyl)-N-((5-chloro-lH-indazol-3-
C126
yl)methyl)nicotinamide
5-(4-(l -acetylpyrrolidin-2-yl)benzyl)-N-((6-fluoro-lH-indol-5-
C127
yl)methyl)nicotinamide
5-(4-(l -acetylpyrrolidin-2-yl)benzyl)-N-((3-chloro-lH-indol-5-
C128
yl)methyl)nicotinamide
5-(4-(l -acetylpyrrolidin-2-yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C129
yl)methyl)nicotinamide
5-(4-(l -acetylpyrrolidin-2-yl)benzyl)-N-((5-chloro- 1 H-indazol-3 -
C130
yl)methyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxoimidazolidin-l-
C131
yl)methyl)benzyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxooxazolidin-3-
C132
yl)methyl)benzyl)nicotinamide
(R)-5-(4-(l -acetamidoethyl)benzyl)-N-((3-chloro-lH-indol-5-
C133
yl)methyl)nicotinamide
(R)-5-(4-(l -acetamidoethyl)benzyl)-N-((5-chloro-lH-indazol-3-
C134
yl)methyl)nicotinamide
N-((3-chloro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyrazin-l (2H)-
C135
yl)methyl)benzyl)nicotinamide
N-((6-(aminomethyl)pyridin-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-
C136
yl)methyl)pyridin-3-yl)methyl)nicotinamide
N-((3-chloro-lH-indol-5-yl)methyl)-l-(2,3-difluoro-4-((2-oxopyrazin-l(2H)-
C137
yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-l -(2,3-difluoro-4-((2-
C138
oxopyrazin-l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
1 -((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3 -chloro- 1 H-indol-5-
C139
yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3-
C140
yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
l-(2,3-difluoro-4-((2-oxopyrazin-l(2H)-yl)methyl)benzyl)-N-((6-fluoro-lH-
C141
indol-5-yl)methyl)-6-oxo-l ,6-dihydropyridine-3-carboxamide
1 -[6-(Acetylamino-methyl)-pyridin-3 -ylmethyl]-6-oxo- 1 ,6-dihydro-pyridine-3 -
C142
carboxylic acid (3 -chloro- 1 H-pyrrolo [2,3 -b]pyridin-5-ylmethyl)-amide l-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((6-fluoro-lH-indol-5-
C143
yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
1 -[6-(Acetylamino-methyl)-pyridin-3 -ylmethyl]-6-oxo- 1 ,6-dihydro-pyridine-3 -
C144
carboxylic acid (3-chloro-6-fluoro-lH-indol-5-ylmethyl)-amide
5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C145
yl)methyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l-(2,3-difluoro-4-((2-
C146
oxopyrrolidin-l -yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l-(2,3-difluoro-4-((2-oxopyrazin-
C147
l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
2-(5-((5-(((5-chloro-lH-indazol-3-yl)methyl)carbamoyl)pyridin-3-yl)methyl)-
C148
lH-indol-3-yl)acetic acid
2-(5-((5 -(((3 -chloro- 1 H-indol-5 -yl)methyl)carbamoyl)pyridin-3 -yl)methyl)- 1 H-
C149
indol-3-yl)acetic acid
5-[6-(Acetylamino-methyl)-pyridin-3 -ylmethyl]-N-(3 -chloro- 1 H-indol-5-
C150
ylmethyl)-nicotinamide 5-[6-(Acetylamino-methyl)-pyridin-3-ylmethyl]-N-(3-chloro-lH-pyrrolo[2,3-
C151
b]pyridin-5-ylmethyl)-nicotinamide
5-[6-(Acetylamino-methyl)-pyridin-3 -ylmethyl]-N-(5-chloro- 1 H-indazol-3 -
C152
ylmethyl)-nicotinamide
5-[6-(Acetylamino-methyl)-pyridin-3-ylmethyl]-N-(3-chloro-6-fluoro-lH-
C153
indol-5-ylmethyl)-nicotinamide
2-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C154
yl)methyl)pyridine-4-carboxamide
6-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C155
yl)methyl)pyrazine-2-carboxamide
5-(4-(acetamidomethyl)benzyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5-
C156
yl)methyl)pyridine-3 -carboxamide
l-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C157
yl)methyl)-l,6-dihydro-6-oxopyridine-3-carboxamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxooxazolidin-3-
C158
yl)methyl)benzyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l -((6-((2-oxopyrazin-
C159
l(2H)-yl)methyl)pyridin-3-yl)methyl)-l,6-dihydropyridine-3-carboxamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l -((6-((2-oxopyrrolidin-l -
C160
yl)methyl)pyridin-3-yl)methyl)-l,6-dihydropyridine-3-carboxamide
5-(4-(acetamidomethyl)-2,3 -difluorobenzyl)-N-((3-chloro- 1 H-indol-5-
C161
yl)methyl)nicotinamide
5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((3-chloro-lH-pyrrolo[2,3-
C162
b]pyridin-5-yl)methyl)nicotinamide
5-(4-(acetamidomethyl)-2,3-difluorobenzyl)-N-((5-chloro-lH-indazol-3-
C163
yl)methyl)nicotinamide
N-((3 -chloro-6-fluoro- 1 H-indol-5-yl)methyl)-5-((6-(tetrahydrofuran-2-
C164
yl)pyridin-3-yl)methyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l-(2,3-difluoro-4-((2-oxopyridin-
C165
l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-l -((6-((2-oxopyridin-
C166
l(2H)-yl)methyl)pyridin-3-yl)methyl)-l,6-dihydropyridine-3-carboxamide
N-((3 -chloro-6-fluoro- 1 H-indol-5 -yl)methyl)-6-oxo- 1 -(4-((2-oxopyridin- 1 (2H)-
C167
yl)methyl)benzyl)- 1 ,6-dihydropyridine-3 -carboxamide
5-((6-(l -acetylpyrrolidin-2-yl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-lH-
C168
indol-5 -yl)methyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxoimidazolidin-l-
C169
yl)methyl)benzyl)nicotinamide
1 -(4-(acetamidomethyl)benzyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5-yl)methyl)-6-
C170
oxo-1, 6-dihydropyridine-3 -carboxamide
l-(4-(acetamidomethyl)-2,5-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C171
yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
5-(4-(acetamidomethyl)-2,5-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C172
yl)methyl)nicotinamide
5-(4-(l -acetylpyrrolidin-2-yl)-3-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-
C173
5 -yl)methyl)nicotinamide
5-(4-(l -acetylpyrrolidin-2-yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C174
yl)methyl)nicotinamide
5-(4-(l -acetylpyrrolidin-2-yl)-2,3-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-
C175
indol-5 -yl)methyl)nicotinamide
5-(4-(l -acetylpyrrolidin-2-yl)-2,5-difluorobenzyl)-N-((3-chloro-6-fluoro-lH-
C176
indol-5 -yl)methyl)nicotinamide N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((6-(5-oxopyrrolidin-2-
C177
yl)pyridin-3-yl)methyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((2-oxopyridin-l(2H)-
C178
yl)methyl)pyridin-3-yl)methyl)isonicotinamide
2-(4-(acetamidomethyl)benzyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5-
C179
yl)methyl)isonicotinamide
5-(4-(acetamidomethyl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C180
yl)methyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-(2-fluoro-4-((2-
C181
hydroxyacetamido)methyl)benzyl)nicotinamide
5-(4-((2-aminoacetamido)methyl)-2-fluorobenzyl)-N-((3 -chloro-6-fluoro- 1 H-
C182
indol-5 -yl)methyl)nicotinamide 2,2,2-trifluoroacetate
2-((6-(acetamidomethyl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-lH-indol-
C183
5-yl)methyl)isonicotinamide 2,2,2-trifluoroacetate
5-(4-(l -acetylpyrrolidin-2-yl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-
C184
5 -yl)methyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l -(3-fluoro-4-((2-oxopyridin-
C185
l(2H)-yl)methyl)benzyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((6-((N-
C186
cyclopropylacetamido)methyl)pyridin-3-yl)methyl)nicotinamide
5-(4-(acetamidomethyl)-5-chloro-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-
C187
indol-5 -yl)methyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((6-((2-
C188
hydroxyacetamido)methyl)pyridin-3-yl)methyl)nicotinamide
5-((6-((2-aminoacetamido)methyl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-
C189
1 H-indol-5 -yl)methyl)nicotinamide
l-(4-(l -acetylpyrrolidin-2-yl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-
C190
5-yl)methyl)-6-oxo-l,6-dihydropyridine-3-carboxamide
5-(4-((2-aminoacetamido)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C191
yl)methyl)nicotinamide
5-((4-(acetamidomethyl)-2-oxopyridin-l(2H)-yl)methyl)-N-((3-chloro-6-fluoro-
C192
1 H-indol-5 -yl)methyl)nicotinamide
5-(4-(acetamidomethyl)-3-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C193
yl)methyl)nicotinamide
2-(4-(acetamidomethyl)-3-methoxybenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C194
yl)methyl)isonicotinamide
N-((3 -chloro-6-fluoro- 1 H-indol-5 -yl)methyl)-2-(4-((2-
C195
hydroxyacetamido)methyl)-3-methoxybenzyl)isonicotinamide
2-(4-((2-aminoacetamido)methyl)-3-methoxybenzyl)-N-((3-chloro-6-fluoro-lH-
C196
indol-5-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-l H-indol-5 -yl)methyl)-5-(4-((2-
C197
hydroxyacetamido)methyl)benzyl)nicotinamide
methyl l -((5-(((3-chloro-6-fluoro-lH-indol-5-yl)methyl)carbamoyl)pyridin-3-
C198
yl)methyl)-lH-pyrazole-4-carboxylate
5-((4-(acetamidomethyl)-lH-pyrazol-l-yl)methyl)-N-((3-chloro-6-fluoro-lH-
C199
indol-5 -yl)methyl)nicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((6-((2-oxopyridin-l(2H)-
C200
yl)methyl)pyridin-3-yl)methyl)isonicotinamide
2-((3-(acetamidomethyl)-lH-pyrazol-l-yl)methyl)-N-((3-chloro-6-fluoro-lH-
C201
indol-5-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C202
yl)methyl)benzyl)isonicotinamide N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-5-((4-((N-
C203
cyclopropylacetamido)methyl)-lH-pyrazol-l -yl)methyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((2-
C204
hydroxyacetamido)methyl)pyridin-3-yl)methyl)isonicotinamide
2-((6-((2-aminoacetamido)methyl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-
C205
1 H-indol-5 -yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((2-oxopyridin-l(2H)-
C206
yl)methyl)pyridin-3-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((N-cyclopropyl-2-
C207
hydroxyacetamido)methyl)pyridin-3-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-((N-
C208
cyclopropylacetamido)methyl)pyridin-3-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-((N-
C209
cyclopropylacetamido)methyl)-lH-pyrazol-l -yl)methyl)isonicotinamide
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((6-((2-oxopyridin-l(2H)-
C210
yl)methyl)pyridin-3-yl)methyl)isonicotinamide
2-((4-(acetamidomethyl)-lH-pyrazol-l-yl)methyl)-N-((3-chloro-6-fluoro-lH-
C211
indol-5-yl)methyl)isonicotinamide
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((6-((N-
C212
cyclopropylacetamido)methyl)pyridin-3-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(pyridin-4-
C213
yl)benzyl)isonicotinamide
2-(4-(acetamidomethyl)-2-fluorobenzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C214
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((methylamino)(4-((2-
C215
oxopyridin- 1 (2H)-yl)methyl)phenyl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-
C216
cyclopropylbenzyl)isonicotinamide
N-((3 -chloro-4-fluoro- 1 H-indol-5 -yl)methyl)-2-(4-((N-
C217
cyclopropylacetamido)methyl)benzyl)isonicotinamide
N-((3 -chloro-6-fluoro- 1 H-indol-5 -yl)methyl)-2-(4-((N-
C218
cyclopropylacetamido)methyl)benzyl)isonicotinamide
2-(amino(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)methyl)-N-((3-chloro-6-
C219
fluoro-lH-indol-5-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(l -methyl-lH-pyrazol-4-
C220
yl)benzyl)isonicotinamide
2-(3-(lH-pyrazol-3-yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C221
yl)methyl)isonicotinamide
2-( [2,4'-bipyridin] -6-ylmethyl)-N-((3 -chloro-6-fluoro- 1 H-indol-5 -
C222
yl)methyl)isonicotinamide
2-(3-(lH-pyrazol-4-yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C223
yl)methyl)isonicotinamide
2-(4-(acetamidomethyl)benzyl)-N-((3 -chloro-4-fluoro- 1 H-indol-5-
C224
yl)methyl)isonicotinamide
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C225
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-
C226
yl)methyl)benzyl)nicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(pyridin-3-
C227
yl)benzyl)isonicotinamide
2-(4-((lH-pyrazol-l-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C228
yl)methyl)isonicotinamide N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(furan-2-
C229
yl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(l -methyl-lH-pyrazol-5-
C230
yl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(pyrimidin-5-
C231
yl)benzyl)isonicotinamide
2-(3-(lH-pyrazol-l -yl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C232
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(isoxazol-4-
C233
yl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(3,5-dimethylisoxazol-4-
C234
yl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-
C235
(hydroxymethyl)benzyl)isonicotinamide
2-((6-((lH-pyrazol-l -yl)methyl)pyridin-3-yl)methyl)-N-((3-chloro-6-fluoro-
C236
1 H-indol-5 -yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-cyano-4-((2-oxopyridin-
C237
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-fluoro-4-((2-oxopyridin-
C238
l(2H)-yl)methyl)benzyl)isonicotinamide
2-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-
C239
1 H-indol-5 -yl)methyl)isonicotinamide
2-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro-6-
C240
fluoro-lH-indol-5-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((4-fluoro-2-oxopyridin-
C241
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3,5-dichloro-2-oxopyridin-
C242
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyrazin-l(2H)-
C243
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((6-oxopyrimidin-l(6H)-
C244
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro- 1 H-indol-5-yl)methyl)-2-(3 -(2-oxopyridin- 1 (2H)-
C245
yl)benzyl)isonicotinamide
N-(4-((4-(((3-chloro-6-fluoro-lH-indol-5-yl)methyl)carbamoyl)pyridin-2-
C246
yl)methyl)benzyl)picolinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-
C247
(isobutyramidomethyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3-
C248
methylureido)methyl)benzyl)isonicotinamide
2-(4-(benzamidomethyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C249
yl)methyl)isonicotinamide
methyl 4-((4-(((3-chloro-6-fluoro-lH-indol-5-yl)methyl)carbamoyl)pyridin-2-
C250
yl)methyl)benzylcarbamate
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-
C251
(cyclopropanecarboxamidomethyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-
C252
(methylsulfonamidomethyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-4-oxo-l -(4-((2-oxopyridin-l(2H)-
C253
yl)methyl)benzyl)- 1 ,4-dihydropyridine-3 -carboxamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-
C254
phenoxybenzyl)isonicotinamide N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-l -((3-((2-oxopyridin-l(2H)-
C255
yl)methyl)bicyclo [1.1.1 Jpentan- 1 -yl)methyl)- 1 H-pyrazole-4-carboxamide
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((6-((2-oxopyridin-
C256
l(2H)-yl)methyl)pyridin-3-yl)methyl)isonicotinamide
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(4-((2-oxopyridin-
C257
l(2H)-yl)methyl)benzyl)isonicotinamide
2-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro-lH-
C258
pyrrolo[2,3-b]pyridin-5-yl)methyl)isonicotinamide
2-(3-(aminomethyl)-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro-6-
C259
fluoro-lH-indol-5-yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-
C260
morpholinobenzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-
C261
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro- 1 H-indol-5-yl)methyl)-2-(4-((l -methyl- 1 H-pyrazol-5-
C262
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3-
C263
cyclopropylureido)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyridin-3-
C264
yloxy)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((4-cyano-2-oxopyridin-
C265
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((5-fluoro-2-oxopyridin-
C266
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro- 1 H-indol-5-yl)methyl)-2-(4-((3 -methyl- 1 H-pyrazol- 1 -
C267
yl)methyl)benzyl)isonicotinamide
2-(4-((lH-imidazol-l-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C268
yl)methyl)isonicotinamide
N-(4-(aminomethyl)benzyl)-2-(4-((2-oxopyridin- 1 (2H)-
C269
yl)methyl)benzyl)isonicotinamide
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C270
yl)methyl)benzyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C271
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-
C272
yl)methyl)benzyl)picolinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxo-5-
C273
(trifluoromethyl)pyridin-l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3-cyano-2-oxopyridin-
C274
l(2H)-yl)methyl)benzyl)isonicotinamide
2-(4-((5-chloro-2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-
C275
1 H-indol-5 -yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxo-3-
C276
(trifluoromethyl)pyridin-l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3-fluoro-2-oxopyridin-
C277
l(2H)-yl)methyl)benzyl)isonicotinamide
2-(4-((3-chloro-2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-
C278
1 H-indol-5 -yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((3,5-dimethyl-lH-pyrazol-
C279
l-yl)methyl)benzyl)isonicotinamide
N-((6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C280
yl)methyl)benzyl)isonicotinamide N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-fluoro-2-oxopyridin-
C281
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((5-fluoro-2-oxopyridin-
C282
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-oxo-4-(4-((2-oxopyridin-l(2H)-
C283
yl)methyl)benzyl)- 1 ,6-dihydropyridine-2-carboxamide
N-((3-chloro-lH-pyrrolo[2,3-c]pyridin-5-yl)methyl)-2-((6-((2-oxopyridin-
C284
l(2H)-yl)methyl)pyridin-3-yl)methyl)isonicotinamide
N-((3-chloro-lH-pyrrolo[2,3-c]pyridin-5-yl)methyl)-2-(4-((2-oxopyridin-
C285
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-chloroquinolin-3-
C286
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-methylquinolin-3-
C287
yl)methyl)isonicotinamide
2-([l,l'-biphenyl]-4-ylmethyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C288
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(furan-2-
C289
ylmethyl)benzyl)isonicotinamide
2-(4-((lH-pyrazol-3-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C290
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro- 1 H-indol-5-yl)methyl)-2-(4-((4-methyl- 1 H-pyrazol- 1 -
C291
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyridin-3-
C292
ylmethyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-(pyrimidin-5-
C293
ylmethyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro- 1 H-indol-5-yl)methyl)-2-(quinolin-3 -
C294
ylmethyl)isonicotinamide
N-((6-amino-5-chloropyridin-3 -yl)methyl)-2-(4-((2-oxopyridin- 1 (2H)-
C295
yl)methyl)benzyl)isonicotinamide
N-((6-amino-5-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C296
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-
C297
oxopyridin- 1 (2H)-yl)methyl)benzyl)isonicotinamide
N-((6-amino-5-methoxypyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l (2H)-
C298
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(3-(methylsulfonyl)-4-
C299
((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((5-oxo-lH-l,2,4-triazol-
C300
4(5H)-yl)methyl)benzyl)isonicotinamide
N-((5-chloro-lH-pyrrolo[2,3-b]pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-
C301
l(2H)-yl)methyl)benzyl)isonicotinamide
N-(4-bromo-2-methylbenzyl)-2-(4-((2-oxopyridin- 1 (2H)-
C302
yl)methyl)benzyl)isonicotinamide
N-((8-aminoquinolin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C303
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-methylquinolin-6-
C304
yl)methyl)isonicotinamide
N-((3 -chloro-6-fluoro- 1 H-indol-5-yl)methyl)-2-((6-phenylpyridin-3 -
C305
yl)methyl)isonicotinamide
N-(4-bromo-3-chlorobenzyl)-2-(4-((2-oxopyridin-l(2H)-
C306
yl)methyl)benzyl)isonicotinamide N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-phenylthiazol-2-
C307
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-
C308
yl)methyl)benzyl)pyridazine-4-carboxamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-methyl-2-
C309
(methylsulfonyl)quinolin-3-yl)methyl)isonicotinamide
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((2-methylquinolin-6-
C310
yl)methyl)isonicotinamide
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((6-methylquinolin-3-
C311
yl)methyl)isonicotinamide
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((6-fluoroquinolin-3-
C312
yl)methyl)isonicotinamide
N-(4-(aminomethyl)-3-fluorobenzyl)-2-(4-((2-oxopyridin-l(2H)-
C313
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((6-fluoroquinolin-3-
C314
yl)methyl)isonicotinamide
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l(2H)-
C315
yl)methyl)benzyl)isonicotinamide
N-((l -aminoisoquinolin-6-yl)methyl)-2-(4-((2-oxopyridin-l (2H)-
C316
yl)methyl)benzyl)isonicotinamide
N-((l -aminoisoquinolin-7-yl)methyl)-2-(4-((2-oxopyridin-l (2H)-
C317
yl)methyl)benzyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6-methylquinolin-3-
C318
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6-fluoroquinolin-3-
C319
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((7-fluoroquinolin-3-
C320
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-
C321
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methylquinolin-6-
C322
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-fluoroquinolin-3-
C323
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-methylquinolin-3-
C324
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methylquinolin-6-
C325
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((4-cyanoquinolin-6-
C326
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-
C327
oxopyridin- 1 (2H)-yl)methyl)benzyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((3-cyano-2-oxopyridin-
C328
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((3-cyano-2-oxopyridin-l(2H)-
C329
yl)methyl)benzyl)isonicotinamide
N-((l -aminoisoquinolin-6-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-
C330
l(2H)-yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((4-cyanoquinolin-6-
C331
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((4-cyanoquinolin-6-
C332
yl)methyl)isonicotinamide N-((l-aminoisoquinolin-6-yl)methyl)-2-((7-fluoroquinolin-3-
C333
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((7-fluoroquinolin-3-
C334
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-(4-((5-cyano-2-oxopyridin-l(2H)-
C335
yl)methyl)benzyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methylquinolin-7-
C336
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-methylquinolin-7-
C337
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methylquinolin-7-
C338
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((7-chloroquinolin-3-
C339
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((7-chloroquinolin-3-
C340
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((7-chloroquinolin-3-
C341
yl)methyl)isonicotinamide
N-((4-aminoquinazolin-7-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-
C342
yl)methyl)benzyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-isocyanoquinolin-6-
C343
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-isocyanoquinolin-6-
C344
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylisoquinolin-6-
C345
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methylisoquinolin-6-
C346
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-(methylsulfonyl)quinolin-6-
C347
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-(methylsulfonyl)quinolin-6-
C348
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylquinolin-6-
C349
yl)methyl)isonicotinamide
N-((3-chloro-6-fluoro- 1 H-indol-5-yl)methyl)-2-((3 -methylquinolin-6-
C350
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-
C351
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methylisoquinolin-6-
C352
yl)methyl)isonicotinamide
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-(aminomethyl)quinolin-6-
C353
yl)methyl)isonicotinamide
2-((2-(aminomethyl)quinolin-6-yl)methyl)-N-((3-chloro-6-fluoro-lH-indol-5-
C354
yl)methyl)isonicotinamide
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-(aminomethyl)quinolin-6-
C355
yl)methyl)isonicotinamide Table 2. Biological activity of several illustrative compounds against human plasma kallikrein hPK Compound No.
C01, C05, C07, C08, CIO, C12, C13, C14, C15, C16, C17, C18, C25, C27, C28, C29, C30, C31, C33, C38, C39, C40, C41, C42, C44, C46, C48, C49, C50, C51, C53, C54, C55, C56, C57, C60, C64, C65, C66, C68, C69, C70, C73, C77, C83, C84, C85, C89, C90, C91, C92, C93, C94, C96, C97, C103, C105, C106, C109, CI 14, C120, C121, C128, C129, C131, C135, C145, C150, C151, C152, C153, C154, C156, C158, C162, C163, C165, C166, C167, C168, C169, C174, C175, C176, C178, C179, C183, C184,
+++ C185, C186, C187, C188, C193, C194, C200, C202, C206, C207, C208, C210, C215, C217, C218, C219, C224, C225, C226, C228, C236, C237, C238, C239, C240, C241, C242, C243, C244, C247, C248, C250, C251, C256, C257, C258, C259, C261, C262, C263, C266, C267, C268, C270, C271, C272, C273, C274, C275, C276, C277, C278, C279, C280, C281, C282, C283, C284, C286, C287, C291, C294, C297, C299, C300, C301, C304, C308, C310, C311, C312, C314, C316, C318, C319, C320, C321, C322, C323, C324, C325, C326, C327, C328, C329, C330, C332, C333, C334, C335, C336, C337, C339, C340, C341, C343, C344, C345, C346, C349, C350, C351, C352, C355
C03, C04, C21, C22, C23, C24, C34, C35, C36, C37, C43, C45, C47, C59, C61, C62, C63, C71, C74, C75, C76, C78, C79, C80, C81, C82, C86, C87, C88, C95, C98,
CIOO, ClOl, C107, C108, C110, Cl l l, C112, C113, C115, C116, C117, C118, C119, C122, C123, C124, C125, C126, C127, C130, C132, C133, C134, C136, C137, C138,
++ C139, C140, C142, C144, C146, C147, C155, C157, C159, C160, C161, C164, C170, C171, C172, C173, C177, C180, C181, C182, C189, C190, C191, C192, C195, C196, C197, C198, C203, C204, C205, C209, C212, C213, C214, C216, C220, C222, C227, C229, C231, C232, C233, C234, C245, C246, C249, C252, C253, C254, C255, C264, C265, C269, C285, C288, C289, C290, C292, C293, C296, C298, C305, C307, C309, C313, C315, C317, C331, C338, C342, C347, C348, C353, C354
C02, C06, C09, Cl l, C19, C20, C26, C32, C52, C58, C67, C72, C99, C102, C104,
+ C141, C143, C148, C149, C199, C201, C211, C221, C223, C230, C235, C260, C295, C302, C303, C306
Example 357: The following illustrate representative pharmaceutical dosage forms, containing a compound of Formula I or II, or a pharmaceutically acceptable salt thereof ('Compound X'), for therapeutic or prophylactic use in humans.
(D Tablet 1 mg/tablet
Compound X= 100.0
Lactose 77.5
Povidone 15.0
Croscarmellose sodium 12.0
Microcrystalline cellulose 92.5
Magnesium stearate 3J)
300.0
(if) Tablet 2 mg/tablet
Compound X= 20.0
Microcrystalline cellulose 410.0
Starch 50.0
Sodium starch glycolate 15.0
Magnesium stearate 5J)
500.0 (iii) Capsule mg/capsule
Compound X= 10.0
Colloidal silicon dioxide 1.5
Lactose 465.5
Pregelatinized starch 120.0
Magnesium stearate 3.0
600.0
(iv) Injection 1 (1 mg/ml) mg/ml
Compound X= (free acid form) 1.0
Dibasic sodium phosphate 12.0
Monobasic sodium phosphate 0.7
Sodium chloride 4.5
1.0 N Sodium hydroxide solution
(pH adjustment to 7.0-7.5) q.s.
Water for injection q.s. ad 1
(v) Injection 2 Π0 mg/ml) mg/ml
Compound X= (free acid form) 10.0
Monobasic sodium phosphate 0.3
Dibasic sodium phosphate 1.1
Polyethylene glycol 400 200.0
1.0 N Sodium hydroxide solution
(pH adjustment to 7.0-7.5) q.s.
Water for injection q.s. ad 1
(vi) Aerosol mg/can
Compound X= 20.0
Oleic acid 10.0
Trichloromonofluoromethane 5,000.0
Dichlorodifluoromethane 10,000.0
Dichlorotetrafluoroethane 5,000.0
The above formulations may be prepared by conventional procedures well known in the pharmaceutical art.
All publications, patents, and patent documents are incorporated by reference herein, as though individually incorporated by reference. The invention has been described with reference to various specific and preferred embodiments and techniques. However, it should be understood that many variations and modifications may be made while remaining within the spirit and scope of the invention.

Claims

What is claimed is:
1. A compound of Formula I:
A-B-C-D-E-F-G-J (I)
wherein:
A is a 5 or 6 membered heterocyclic ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
B is absent or is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci- C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(G- C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
C is phenyl or heteroaryl, which phenyl or heteroaryl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, (Ci- C6)alkyl-NRaRb, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
D is absent, (Ci-C3)alkyl, halo(Ci-C3)alkyl, hydroxy(Ci-C3)alkyl, alkoxy(Ci-C3)alkyl, O, S, S(=0)2, - CH(NRaRb)-, or NRa;
E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
F is
Figure imgf000378_0001
when E is a 5-membered heteroaryl ring; or F is -C(=0)-NRa- and C is an unsubstituted phenyl when E is a 5-membered heteroaryl ring; or F
is -S(=0)2NRa-, -NRaS(=0)2-, -NRa-C(=0)-, -C(COORa)-, -C(CF3)- or -C(=0)-NRa- when E has any value other than a 5-membered heteroaryl ring;
G is absent or is (Ci-C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(G- C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(G- C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
J is aryl or heteroaryl, which aryl or heteroaryl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3- C6)carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci- Ce)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-
C6)alkanoyloxy, -C(=0)ORa, -NRaRb, -C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, -C(=0)NRaRb, or -C(=NRa)NRaRb; or J is -0-NH-C(NH)-NH2;
each Ra is independently H, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or -C(=0)NRcRd, wherein each (Ci-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or - C(=0)NRcRd is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci- Ce)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rb is independently H or (Ci-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rc is independently H or (Ci-Ce)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rd is independently H or (Ci-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb; and
each Re is independently H or (Ci-Ce)alkyl;
or a salt thereof.
2. The compound of claim 1 wherein A is a piperidine, indazole, morpholine, piperazine, oxazolidine, imidazole, or pyrrolidine, that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci- Ce)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb.
3. The compound of claim 1 wherein A is a piperidine, indazole, morpholine, piperazine, oxazolidine, imidazole, or pyrrolidine, that is substituted with oxo and that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (G-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb.
4. The compound of claim 1 wherein A is a 2-oxopiperidin-l -yl, pyrazole-l -yl, 3-oxomorpholino, 2- oxooxazolidin-3-yl, 2-oxoimidazolidin-l-yl, or 2-oxopyrrolidin-l -yl.
5. The compound of any one of claims 1-4 wherein B is CH2.
6. The compound of any one of claims 1-5 wherein C is phenyl or a 6-membered heteroaryl ring, which phenyl or 6-membered heteroaryl ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (G-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
7. The compound of any one of claims 1-5 wherein C is pyridine, pyrimidine, pyrazine, pyridazine, or triazine, wherein any pyridine, pyrimidine, pyrazine, pyridazine, or triazine is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (G-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (G- C6)alkanoyl, halo(G-C6)alkyl, hydroxy(G-C6)alkyl, (G-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
8. The compound of any one of claims 1-5 wherein C is phenyl or pyridyl where any phenyl or pyridyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (G- G)alkyl, (G-G)carbocyclyl, (G-G) carbocyclyl(G-G)alkyl, (G-G)alkoxy, halo(G-G)alkoxy, (G- Ce)alkenyl, (G-G)alkynyl, (G-G)alkanoyl, halo(G-C6)alkyl, hydroxy(G-C6)alkyl, (G-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
9. The compound of any one of claims 1-5 wherein C is phenyl or pyridyl, where any phenyl or pyridyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, cyano, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
10. The compound of any one of claims 1-5 wherein C is phenyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, cyano, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
11. The compound of any one of claims 1 -5 wherein C is pyridyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, cyano, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
12. The compound of any one of claims 1-5 wherein C is phenyl, aminophenyl, fluorophenyl, chlorophenyl, cyanophenyl, aminocarbonylphenyl, hydroxyphenyl, pyridyl, or aminopyridyl.
13. A compound of Formula II:
C-D-E-F-G-J (II)
wherein:
C is a bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (G-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -(Ci-C6)alkyl-COOH, - (Ci-C6)alkyl-CO-(Ci-C6)alkoxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
or C is a monocyclic carbocyclic ring or a monocyclic heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6)carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, -(Ci-C6)alkyl-NRaRb, -(Ci-C6)alkyl-NH-C(0)-NRaRb, - (Ci-C6)alkyl-NH-C(NH)-NRaRb, -(Ci-C6)alkyl-CO-(Ci-C6)alkoxy, -(Ci-C6)alkyl-COOH, -(Ci-C6)alkyl-CO- NRaRb, -(Ci-C6)alkoxy-CO-NRaRb, S(=0)2Re, and -C(=0)NRaRb;
D is absent, (Ci-C3)alkyl, halo(Ci-C3)alkyl, O, S, S(=0)2, -CH(NRaRb)-, or NRa;
E is a carbocyclic ring or a heterocyclic ring, which carbocyclic ring or a heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
F is -S(=0)2NRa-, -NRa S(=0)2-, -NRa-C(=0)-, or -C(=0)-NRa-;
G is absent or is (Ci-C3)alkyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
J is bicyclic 8-12 membered heteroaryl ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (G-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, - C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, - C(=0)NRaRb, or -C(=NRa)NRaRb;
or J is a monocyclic heterocyclic ring, which heterocyclic ring is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (G-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6)carbocyclyl(Ci-C6)alkyl, (G-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (G- Ce)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, - (Ci-C6)alkyl-NRaRb, -(Ci-C6)alkyl-NH-C(0)-NRaRb, -(Ci-C6)alkyl-NH-C(NH)-NRaRb, -(Ci-C6)alkyl-CO-(Ci- C6)alkoxy, -(Ci-C6)alkyl-COOH, -(Ci-C6)alkyl-CO-NRaRb, -(Ci-C6)alkoxy-CO-NRaRb, S(=0)2Re, and - C(=0)NRaRb;
each Ra is independently H, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2-, or -C(=0)NRcRd, wherein each (Ci-C6)alkyl, (C3-C6)carbocyclyl, (Ci-C6)alkanoyl, (Ci-C6)alkylS(0)2- or - C(=0)NRcRd is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci- Ce)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl,
(Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rb is independently H or (Ci-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb;
each Rc is independently H or (Ci-Ce)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (G-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb;
each Rd is independently H or (Ci-C6)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and - C(=0)NRaRb; and
each Re is independently H or (Ci-Ce)alkyl;
or a salt thereof.
14. The compound of claim 13 having the Formula lib:
Figure imgf000382_0001
Formula lib
wherein each Xi, X2, X3 and X4 is independently CH or N; and G is (Ci_C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3- Ce)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2- C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb. compound of claim 13 having the Formula lie
Figure imgf000383_0001
J Formula lie;
wherein each of Xi, X2, X3 and X4 is independently CH or N; and G is (Ci.C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb. compound of claim 1 having the Formula lb
Figure imgf000383_0002
Formula |b
wherein each Xi, X2, X3 and X4 is independently are CH or N; and G is (Ci_C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, and (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb. compound of claim 1 having the Formula Ic
Figure imgf000383_0003
J Formula Ic;
wherein each of Xi, X2, X3 and X4 is independently CH or N; and G is (Ci_C3)alkyl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci- C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
18. The compound of any one of claims 13-17 wherein C is a quinoline that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
19. The compound of any one of claims 13-17 wherein C is a 6-quinoline that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
20. The compound of any one of claims 13-17 wherein C is a 2-methyl-6-quiolinyl.
21. The compound of any one of claims 1 -20 wherein D is (Ci-C3)alkyl.
22. The compound of any one of claims 1 -20 wherein D is methylene.
23. The compound of any one of claims 1 -13 wherein E is a carbocyclic ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
24. The compound of any one of claims 1 -13 wherein E is a heterocyclic ring that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, S(=0)2Re, and -C(=0)NRaRb.
25. The compound of any one of claims 1 -13 wherein E is pyrazole, pyridine, indole, phenyl, morpholine, pyrimidine, pyrazine, pyradizine, 2-oxooxazolidine, pyrrolidine, 2-oxopyrrolidine, piperazine, oxopiperazine, oxopyridine, oxopyrimidine, oxopiperidine, oxomorpholine, azaspiro[3,4]octane, 5-thia-2- azaspiro[3,4]octane, hydroxypyridine, indazole, lH-pyrrolo[2,3-b]pyridine, or hydroxypyrimidine, wherein E is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2- Ce)alkenyl, (C2-Ce)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy-NRaRb, S(=0)2Re, and -C(=0)NRaRb.
26. The compound of any one of claims 1-13 wherein E is pyrazole, pyridine, or phenyl.
27. The compound of any one of claims 1-13 wherein E is pyrazole.
28. The compound of any one of claims 1-13 wherein E is pyridine.
29. The compound of any one of claims 1-13 wherein F is
Figure imgf000385_0001
30. The compound of any one of claims 1-13 wherein F is -NRa S(=0)2-.
31. The compound of any one of claims 1-13 wherein F is -NRa-C(=0)-.
32. The compound of any one of claims 1-13 wherein F is -C(=0)-NRa-.
33. The compound of any one of claims 1-32 wherein G is (Ci-C3)alkyl is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, oxo, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb, and -C(=0)NRaRb.
34. The compound of any one of claims 1-32 wherein G is methylene.
35. The compound of any one of claims 1-34 wherein J is indazole that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci- Ce)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-
C6)alkanoyloxy, -C(=0)ORa, -NRaRb, -C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, -C(=0)NRaRb, or -C(=NRa)NRaRb.
36. The compound of any one of claims 1-34 wherein J is indazole-3-yl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3- C6)carbocyclyl, (C3-C6) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-Ce)alkenyl, (C2- C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2- C6)alkanoyloxy, -NRaRb,-C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, NRaRb, or -C(=0)NRaRb.
37. The compound of any one of claims 1-34 wherein J is 5-chloro-indazole-3-yl.
38. The compound of any one of claims 1-34 wherein J is indole, pyridine, azaindole, quinoline, or isoquinoline that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -C(=0)ORa, -NRaRb, -C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci- C6)alkyl that is optionally substituted with -C(=0)ORa, -NRaRb, -C(=0)NRaRb, or -C(=NRa)NRaRb.
39. The compound of any one of claims 1-34 wherein J is l-aminoisoquinolin-6-yl, 3-chloro-6-fluoro-lH- indol-5-yl, 6-amino-2,4-dimethylpyridin-3-yl, 3-chloro-4-fluoro-lH-indol-5-yl, or 3-chloro-lH-pyrrolo[2,3- b]pyridin-5-yl that is optionally substituted with one or more groups independently selected from halo, hydroxyl, nitro, cyano, (Ci-C6)alkyl, (C3-C6)carbocyclyl, (C3-Ce) carbocyclyl(Ci-C6)alkyl, (Ci-C6)alkoxy, halo(Ci-C6)alkoxy, (C2-C6)alkenyl, (C2-C6)alkynyl, (Ci-C6)alkanoyl, halo(Ci-C6)alkyl, hydroxy(Ci-C6)alkyl, (Ci-C6)alkoxycarbonyl, (C2-C6)alkanoyloxy, -NRaRb,-C(=0)NRaRb, -C(=NRa)NRaRb, and (Ci-C6)alkyl that is optionally substituted with -C(=0)ORa, NRaRb, or -C(=0)NRaRb.
40. The compound of claim 14 or 16 wherein Xi, X2, X3 and X4 are CH.
41. The compound of claim 14 or 16 wherein Xi is N, and X2, X3 and X4 are CH.
The compound of claim 14 or 16 wherein Xi and X3 are N, and X2 and X4 are CH.
The compound of claim 14 or 16 wherein Xi and X4 are N, and X2 and X3 are CH.
The compound of claim 14 or 16 wherein Xi and X2 are N, and X3 and X4 are CH.
45. A compound selected from:
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4- ((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)pyridine-2-sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4- ((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4- ((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)picolinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4- ((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4- ((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)picolinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4- ((2-oxopyridin-l(2H)-yl)methyl)phenoxy)picolinamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)- 1 -(4- ((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-indole-3- carboxamide;
N-((5-chloro-l H-indazol-3 -yl)methyl)-4-(4- ((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-2- carboxamide; methyl 2-(5-chloro-3-((l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4-carboxamido)methyl)- 1 H-indazol- 1 -yl)acetate;
2-(5-chloro-3 -((1 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H-pyrazole-4-carboxamido)methyl)-l H- indazol-l-yl)acetic acid;
N-((l -(2-amino-2-oxoethyl)-5-chloro-lH-indazol-3-yl^
1 H-pyrazole-4-carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)isonicotinami
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)- benzenesulfonamide; N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)pyridine-3- sulfonamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(methyl(4-((2-oxopyridin-l (2H)-yl)methyl)phenyl)amino)- benzenesulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)morpholine-2- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrimidine-2- carboxamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-6-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)pyrazine-2-carboxamide; N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridine-4-sulfonamide; N-((5-chloro-lH-indazol-3-yl)methyl)-2-oxo-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)oxazolidine-5- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-l-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)- pyrrolidine-3- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-oxo-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrrolidine-3- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(methyl(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)- amino)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(N-(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl) acetamido)benzamide ;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-5-(methyl(4-((2-oxopyridin- 1 (2H)- yl)methyl)phenyl) amino)nicotinamide ;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(N-(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)- acetamido)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(N-(4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)- acetamido)picolinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyridine-2-sulfonamide; N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)phenoxy)pyridine sulfonamide;
N-(3-(N-((5-chloro-lH-indazol-3-yl)methyl)sulfamoyl)phenyl)-N-(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)acetamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-((4-((2-oxop
sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)amino)pyridine-4- sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrimidine-4- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrimidine-4- carboxamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)pyrrolidine- 1 - carboxamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)pyrrolidine- 1 - sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)pyrrolidine-3- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-oxo-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)oxazolidine-5- sulfonamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-l- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)morpholine-4- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-methyl-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l - carboxamide;
4-acetyl-N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperazine-l- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-oxo-5-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)piperazine-l - carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-methyl-3-oxo-5-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)piperazine- 1 -carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-oxo-l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)piperidine-3- carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)morpholine-2- carboxamide; N-((5-chloro-lH-indazol-3-yl)methyl)-l-((4-((2-oxopyridm^
carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-4-((4-((2-oxopyridm^
carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyri^
carboxamide;
N-((5-chloro H-indazol-3-yl)methyl)-2-(4-((2-oxopyridin (2H)-yl)methyl)benzyl)-5-thia-2- azaspiro [3.4] octane-8 -carboxamide 5,5-dioxide;
2-(5 -chloro- 1 H-indazol-3 -yl)-2-( 1 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H-pyrazole-4- carboxamido)acetic acid;
methyl 2-(5-chloro-lH-indazol-3-yl)-2-(l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)acetate;
N-(2-amino -(5-chloro H-indazol-3-yl)-2-oxoethyl)-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH- pyrazole-4 -carboxamide;
3 -(5 -chloro- 1 H-indazol-3 -yl)-3 -( 1 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)- 1 H-pyrazole-4- carboxamido)propanoic acid;
methyl 3-(5-chloro-lH-indazol-3-yl)-3-(l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH-pyrazole-4- carboxamido)propanoate;
N-(3-amino-l -(5-chloro-lH-indazol-3-yl)-3-oxopropyl)-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-lH- pyrazole -4 -carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-6-oxo-l -(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-l,6- dihydropyridine-3 -carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-oxo-l-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-l,2- dihydropyrimidine-5-carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-oxo-4-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-4,5- dihydropyrazine-2-carboxamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-((4-((2-oxopyridin-l(2H)-yl)methyl)phenyl)sulfonyl)benzamide; and
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(difluoro(4-((2-oxopyridin-l(2H)- yl)methyl)phenyl)methyl)benzamide;
N-(4-carbamimidoylbenzyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-(4-(aminomethyl)benzyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-aminopyridin-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide; N-((6-chloro- 1 H-indazol-3 -yl)methyl)-3 -(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)benzamide;
N-((5-chlorobenzo[b]thiophen-3-yl)methyl)-3-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)benzamide;
N-((6-chlorobenzo[b]thiophen-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloro- 1 H-indol-3 -yl)methyl)-3-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)benzamide;
N-((5-chloro- 1 H-indol-3 -yl)methyl)-3-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)benzamide; N-((7-chloronaphthalen-2-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloronaphthalen-2-yl)methyl)-3 -(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)benzamide;
N-((l -fluoronaphthalen-2-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloro-l -fluoronaphthalen-2-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloroisoquinolin-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide;
N-((6-chloroquinolin-3-yl)methyl)-3-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide; and
N-((2-chlorobenzo[b]thiophen-5-yl)methyl)-3-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)benzamide;
N-(4-carbamimidoylbenzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-(4-(aminomethyl)benzyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-aminopyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)- nicotinamide;
N-((6-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((5-chlorobenzo[b]thiophen-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-chlorobenzo[b]thiophen-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-chloro- 1 H-indol-3 -yl)methyl)-5-(4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indol-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((7-chloronaphthalen-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-chloronaphthalen-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((l-fluoronaphthalen-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-chloro-l -fluoronaphthalen-2-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)- nicotinamide;
N-((6-chloroisoquinolin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide;
N-((6-chloroquinolin-3-yl)methyl)-5-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinamide; and
N-((2-chlorobenzo[b]thiophen-5-yl)methyl)-5-(4-((2-oxopyridin-l (2H)-yl)methyl)benzyl)nicotinamide;
3-(4-((lH-pyrazol-l -yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopiperidin-l-yl)methyl)benzyl)benzamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(4-((3-oxomorpholino)methyl)benzyl)benzamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(4-((2-oxopiperazin- 1 -yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxooxazolidin-3-yl)methyl)benzyl)benzamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3 -(4-((2-oxoimidazolidin- 1 -yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(4-((2-oxopyrrolidin-l -yl)methyl)benzyl)benzamide;
N-((5 -chloro- 1 H-indazol-3 -yl)methyl)-3 -((2-methylquinolin-6-yl)methyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)benzamide; N-((5-chloro-lH-indazol-3-yl)methyl)-3-((5-((2-oxopyridin-l(2H)-yl)methyl)pyridin-2-yl)methyl)benzamide; 3-((6-amino-5-((2-oxopyridin-l(2H)-yl)methyl)pyridin-2-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)benzamide;
3-((2-amino-6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3-yl)methyl)-N-((5-chloro-lH-indazol-3- yl)methyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(2-fluoro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamide; N-((5-chloro-l H-indazol-3 -yl)methyl)-3 -(2 -chloro-4^
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(2-cyan^
3-(2-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)be^
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(2-hydroxy-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzami
3-(2-amino-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)benzam
N-((5-chloro- 1 H-indazol-3-yl)methyl)-3 -(3-fluoro-4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)benzamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(3-chloro-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzamid
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-3-(3 -cyano-4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)benzamide;
3-(3-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)benzam
N-((5-chloro-lH-indazol-3-yl)methyl)-3-(3-hydroxy-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)benzami
3-(3-amino-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)benzamM
5-(4-((lH-pyrazol-l-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3-yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopiperidin-l -yl)methyl)benzyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((3-oxom
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopiperazin-l -yl)methyl)benzyl)nicotinami
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxooxazolidin-3-yl)methyl)benzyl)nicotinami
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxom
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(4-((2-oxopyrrolidin-l-yl)methyl)benzyl)nicotinamid
N-((5-chloro-lH-indazol-3-yl)methyl)-5-((2-methylquinolin-6-yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-((6-((2-oxopyridin-l(2H)-yl)methyl)pyridin-3- yl)methyl)nicotinamide ;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-5-((5-((2-oxopyridin- 1 (2H)-yl)methyl)pyridin-2- yl)methyl)nicotinamide;
5-((6-amino-5-((2-oxopyridin-l(2H)-yl)methyl)py^
yl)methyl)nicotinamide;
5-((2-amino-6-((2-oxopyridin- 1 (2H)-yl)methyl)pyridin-3 -yl)methyl)-N-((5-chloro- 1 H-indazol-3- yl)methyl)nicotinamide;
N-((5-chloro-l H-indazol-3 -yl)methyl)-5-(2-fluoro^
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-chte
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-cyano-4^
5-(2-carbamoyl-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-N-((5-chloro-lH-indazol-3- yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(2-hydroxy-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotinam
5-(2-amino-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)-^
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-flu^
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3^
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-cyano-4^
5-(3 -carbamoyl -4-((2-oxopyridin- 1 (2H)-yl)methyl)benzyl)-N-((5 -chloro- 1 H-indazol-3 - yl)methyl)nicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-5-(3-hydroxy-4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)nicotm^
5- (3-amino-4-((2-oxopyridin-l(2H)-yl)methyl)ben
N-((l -aminoisoquinolin-6-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-cyanoquinolin-3-yl)methyl)isonicotinamide;
3-((4-(((l-aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)quinoline-6-carboxamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-chloroquinolin-3-yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-(hyd
N-((l-aminoisoquinolin-6-yl)methyl)-2-((6-(aminomethyl)quinolin-3-yl)methyl)isonicotinam
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinamide;
6- ((4-(((l-aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)quinoline-3-carboxamide; N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-(hyd
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-(aminomethyl)quinolin-6-yl)methyl)isonicotinam
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6-yl)methyl)isom
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methyl-8-sulfamoylquinolin-6-yl)methyl)isonicotm
6-((4-(((l-aminoisoquinolin-6-yl)methyl)carbamoyl)py
acid;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolm^ yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6-yl)methyl)isonicoti^
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quino yl)methyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-methyl-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)qm yl)methyl)isonicotinamide;
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolm yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6-yl)methyl)isoni N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-fluo
6-((4-(((l-aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)-3-fluoroquinoline-8-carboxyli acid;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolm^ yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6-yl)methyl)isonicotm^
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridm^
yl)methyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-methyl-2-(trifluoromethyl)pyridm^
yl)methyl)isonicotinamide;
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide; N-((6-amino-2-methyl-5-(trifluoromethyl)pyridm
yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-fluoro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamid
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isoni^
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((6-fluoro-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((6-fluoro-lH-indazol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinami
N-((3-chloro-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((5-chloro-lH-indol-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-6-methyl-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinami
N-((3-chloro-6-methyl-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide;
N-((3-chloro-lH-pyrrolo[3,2-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinam
N-((lH-pyrrolo[3,2-b]pyridin-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinam
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinamide;
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinam
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)ison^
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-chloro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-chloro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-chloro-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-chloro-8-(methylsulfonyl)quinolin-6-yl)methyl)isonicoti^ N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isom
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotim
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-chloroquinolin-6-yl)methyl)isonicotinamide; N-((6-fluoro-lH-indol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((6-fluoro-lH-indazol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-lH-indol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((5-chloro-lH-indol-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-6-methyl-lH-indol-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-6-methyl H-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6- yl)methyl)isonicotinamide;
N-((3-chloro-lH-pyrrolo[3,2-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicoti^
N-((lH-pyrrolo[3,2-b]pyridin-5-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotm^
N-((lH-benzo[d]imidazol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((3-aminobenzo[d]isoxazol-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((3-amino-lH-indazol-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((2-aminobenzo[d]oxazol-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((2-aminobenzo[d]oxazol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((6-amino-4-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-(3-(me lsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((3-chloro H-indol-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin-6- yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methyl-8-sulfamoylisoquinolin-6-yl)methyl)isoni^
6-((4-(((l-aminoisoquinolin-6-yl)methyl)carbamoyl)py
acid;
N-((3-chloro H-pyrrolo[2,3 )]pyridin-5-yl)methy^
yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin-6- yl)methyl)isonicotinamide; N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((3-methyl-8-(methylsulfonyl)isoquinolin- yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6-yl)methyl)iso
N-((l-aminoisoquinolin-6-yl)methyl)-2-((2-methyl-8-sulfamoylquinolin-6-yl)methyl)isonicotm
6-((4-(((l -aminoisoquinolin-6-yl)methyl)carbamoyl)pyridin-2-yl)methyl)-2-methylquinoline-8-carbo acid;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)qum^
yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-((2 -methyl
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((2-methyl-8-(methylsulfonyl)quinolin-6- yl)methyl)isonicotinamide;
N-((3-chloro-lH-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyrid
yl)methyl)benzyl)isonicotinamide;
N-((5-chloro-lH-indazol-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((5-(methylsulfonyl)-6-((2-oxopyridin-l(2H)- yl)methyl)pyridin-3-yl)methyl)isonicotinamide;
N-((3-chloro H-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-((5-(methylsulfonyl)-6-((2-oxopyridin ( yl)methyl)pyridin-3-yl)methyl)isonicotinamide;
N-((5-chloro- 1 H-indazol-3 -yl)methyl)-2-((5-(methylsulfonyl)-6-((2-oxopyridin- 1 (2H)-yl)methyl)pyridin-3 - yl)methyl)isonicotinamide;
N-((6-amino-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyri l(2H)-yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-methyl-2-(trifluoromethyl)pyri^
l(2H)-yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)^
yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-chloro-2-methylpyridin-3-yl)m
yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin l(2H)-yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-methyl-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinam
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotm N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotm N-((6 -amino -2 -methyl-5 -(trifluoromethyl)pyridin-3 -yl)methyl) -2 -((3 -methylquinolin-6 - yl)methyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinam N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotina N-((6-amino-4-methyl-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-methylquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-4-cyano-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinamide
N-((6-amino-2-methyl-4-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-fluoroquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-fluoro-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinam N-((6-amino-5-chloro-2-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinam
N-((6-amino-2-methyl-5-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-fluoroquinolin-6- yl)methyl)isonicotinamide;
N-((6-amino-5-cyano-2-methylpyridin-3-yl)methyl)-2-((3-^
N-((6-amino-2-cyano-4-methylpyridin-3-yl)methyl)-2-((3-fluoroquinolin-6-yl)methyl)isonicotinam
N-((6-amino-4-methyl-2-(trifluoromethyl)pyridin-3-yl)methyl)-2-((3-fluoroquinolin-6- yl)methyl)isonicotinamide;
and salts thereof.
46. A compound recited in Table 1 of the specification.
47. The compound of claim 46 wherein the compound has Compound No. C01, C05, C07, C08, CIO, C12, C13, C14, C15, C16, C17, C18, C25, C27, C28, C29, C30, C31, C33, C38, C39, C40, C41, C42, C44, C46, C48, C49, C50, C51, C53, C54, C55, C56, C57, C60, C64, C65, C66, C68, C69, C70, C73, C77, C83, C84, C85, C89, C90, C91, C92, C93, C94, C96, C97, C103, C105, C106, C109, CI 14, C120, C121, C128, C129, C131, C135, C145, C150, C151, C152, C153, C154, C156, C158, C162, C163, C165, C166, C167, C168, C169, C174, C175, C176, C178, C179, C183, C184, C185, C186, C187, C188, C193, C194, C200, C202, C206, C207, C208, C210, C215, C217, C218, C219, C224, C225, C226, C228, C236, C237, C238, C239, C240, C241, C242, C243, C244, C247, C248, C250, C251, C256, C257, C258, C259, C261, C262, C263, C266, C267, C268, C270, C271, C272, C273, C274, C275, C276, C277, C278, C279, C280, C281, C282, C283, C284, C286, C287, C291, C294, C297, C299, C300, C301, C304, C308, C310, C311, C312, C314, C316, C318, C319, C320, C321, C322, C323, C324, C325, C326, C327, C328, C329, C330, C332, C333, C334, C335, C336, C337, C339, C340, C341, C343, C344, C345, C346, C349, C350, C351, C352, C355, or a salt thereof.
48. The compound of claim 1 wherein the compound is:
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((3-chloro-6-fluoro H-indol-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide; N-((3 -chloro-6-fluoro- 1 H-indol-5 -yl)methyl)-2-((6-((2-oxopyridin- 1 (2H)-yl)methyl)pyridin-3 - yl)methyl)isonicotinamide;
N-((6-amino-2-methylpyridin-3-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotinamide;
N-((3-chloro H-pyrrolo[2,3-b]pyridin-5-yl)methyl)-2-(3-(methylsulfonyl)-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-(4-((5-fluoro-2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide; N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotm^
2-(3-chloro-4-((2-oxopyridin (2H)-yl)methyl)benzyl)-N-((3-chloro-6-fluoro-lH-indol-5- yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-(3-fluoro-4-((2-oxopyridin-l(2H)- yl)methyl)benzyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-((6-((2-oxopyridin (2H)-yl)methyl)pyri
yl)methyl)isonicotinamide;
N-((3-chloro-4-fluoro-lH-indol-5-yl)methyl)-2-(4-((2-oxopyridin-l(2H)-yl)methyl)benzyl)isonicotm^
or a salt thereof.
49. The compound of claim 13 wherein the compound is:
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((6-methylquinolin-3-yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((6-amino-2,4-dimethylpyridin-3-yl)methyl)-2-((2-methylquinolin-6-yl)methyl)isonicotinamide;
N-((l-aminoisoquinolin-6-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
N-((3-chloro-6-fluoro-lH-indol-5-yl)methyl)-2-((3-methylquinolin-6-yl)methyl)isonicotinamide;
or a salt thereof.
50. A pharmaceutical composition comprising a compound of Formula I or Formula II, as described in any one of claims 1 -49, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
51. A method of treating a disease or condition in an animal wherein inhibition of plasma kallikrein is indicated comprising administering a compound of Formula I or Formula II, as described in any one of claims 1 -49, or a pharmaceutically acceptable salt thereof, to the animal.
52. A compound of Formula I or Formula II as described in any one of claims 1-49, or a pharmaceutically acceptable salt thereof, for use in medical therapy.
53. A compound of Formula I or Formula II as described in any one of claims 1-49, or a pharmaceutically acceptable salt thereof, for the prophylactic or therapeutic treatment of a disease or condition wherein inhibition of plasma kallikrein is indicated.
54. The use of a compound of Formula I or Formula II as described in any one of claims 1-49, or a pharmaceutically acceptable salt thereof, to prepare a medicament for treatment of a disease or condition in an animal wherein inhibition of plasma kallikrein is indicated.
PCT/US2014/072851 2013-12-30 2014-12-30 Therapeutic inhibitory compounds WO2015103317A1 (en)

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KR1020167020567A KR20160106627A (en) 2013-12-30 2014-12-30 Therapeutic inhibitory compounds
MX2016008688A MX2016008688A (en) 2013-12-30 2014-12-30 Therapeutic inhibitory compounds.
EA201691354A EA032713B1 (en) 2013-12-30 2014-12-30 Therapeutic inhibitory compounds
AU2014373735A AU2014373735B2 (en) 2013-12-30 2014-12-30 Therapeutic inhibitory compounds
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CA2935683A CA2935683A1 (en) 2013-12-30 2014-12-30 Therapeutic inhibitory compounds
BR112016015449A BR112016015449A8 (en) 2013-12-30 2014-12-30 therapeutic inhibitory compounds, pharmaceutical composition comprising them and use thereof
JP2016544593A JP6759100B2 (en) 2013-12-30 2014-12-30 Therapeutic inhibitor compound
AU2015289643A AU2015289643B2 (en) 2014-07-16 2015-07-15 Therapeutic inhibitory compounds
EA201790170A EA035410B1 (en) 2014-07-16 2015-07-15 Therapeutic inhibitory compounds
MX2017000450A MX2017000450A (en) 2014-07-16 2015-07-15 Therapeutic inhibitory compounds.
KR1020177004192A KR102286305B1 (en) 2014-07-16 2015-07-15 Therapeutic inhibitory compounds
BR112017000816A BR112017000816A2 (en) 2014-07-16 2015-07-15 therapeutic inhibitory compounds
CA2954814A CA2954814A1 (en) 2014-07-16 2015-07-15 Isonicotinamide compounds and their use as plasma kallikrein inhibitors
PCT/US2015/040659 WO2016011209A1 (en) 2014-07-16 2015-07-15 Therapeutic inhibitory compounds
JP2017501403A JP6951970B2 (en) 2014-07-16 2015-07-15 Therapeutic inhibitor compound
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