WO2015100473A1 - Procédé et appareil de séquençage - Google Patents

Procédé et appareil de séquençage Download PDF

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Publication number
WO2015100473A1
WO2015100473A1 PCT/AU2014/050446 AU2014050446W WO2015100473A1 WO 2015100473 A1 WO2015100473 A1 WO 2015100473A1 AU 2014050446 W AU2014050446 W AU 2014050446W WO 2015100473 A1 WO2015100473 A1 WO 2015100473A1
Authority
WO
WIPO (PCT)
Prior art keywords
template molecules
reads
digested
template
tier
Prior art date
Application number
PCT/AU2014/050446
Other languages
English (en)
Inventor
Gene TYSON
Philip Hugenholtz
Mike IMELFORT
Margaret Butler
Original Assignee
The University Of Queensland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2014900005A external-priority patent/AU2014900005A0/en
Application filed by The University Of Queensland filed Critical The University Of Queensland
Publication of WO2015100473A1 publication Critical patent/WO2015100473A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Definitions

  • the method includes differentially digesting copies of the at least one template molecule by controlling at least one of:
  • the method includes:
  • the method includes:
  • the method includes:
  • a) determines read data indicative of reads, the reads representing the sequence content of at least an end portion of digested template molecules, the digested template molecules being obtained by differentially digesting a number of copies of at least one template molecule so that at least some of the digested template molecules have different numbers of nucleotides removed from at least one end of the at least one template molecule; and, b) uses the read data to generate consensus sequence data representing the consensus sequence.
  • Figures 4 A to 4C are a flow chart of a specific example of a method for sequencing multiple template DNA fragments

Abstract

L'invention porte sur un procédé de séquençage d'au moins une molécule d'acide nucléique matrice, le procédé comprenant la digestion de façon différentielle d'un certain nombre de copies de ladite ou desdites molécules matrices pour de cette manière produire des molécules matrices digérées, au moins une partie des molécules matrices digérées ayant des nombres différents de nucléotides enlevés d'au moins une extrémité de la molécule matrice ; le séquençage d'au moins une partie des molécules matrices digérées pour produire des lectures représentant la teneur de séquences d'au moins une partie d'extrémité des molécules matrices digérées ; et la production d'une séquence consensus pour ladite ou lesdites molécules matrices à l'aide de chevauchements entre les lectures.
PCT/AU2014/050446 2014-01-02 2014-12-24 Procédé et appareil de séquençage WO2015100473A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2014900005A AU2014900005A0 (en) 2014-01-02 Sequencing method and apparatus
AU2014900005 2014-01-02

Publications (1)

Publication Number Publication Date
WO2015100473A1 true WO2015100473A1 (fr) 2015-07-09

Family

ID=53492844

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2014/050446 WO2015100473A1 (fr) 2014-01-02 2014-12-24 Procédé et appareil de séquençage

Country Status (1)

Country Link
WO (1) WO2015100473A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6248569B1 (en) * 1997-11-10 2001-06-19 Brookhaven Science Associates Method for introducing unidirectional nested deletions
US6258571B1 (en) * 1998-04-10 2001-07-10 Genset High throughput DNA sequencing vector
WO2009052214A2 (fr) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Analyse de séquence à l'aide d'acides nucléiques décorés
WO2012050920A1 (fr) * 2010-09-29 2012-04-19 Illumina, Inc. Compositions et procédés de séquençage d'acides nucléiques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6248569B1 (en) * 1997-11-10 2001-06-19 Brookhaven Science Associates Method for introducing unidirectional nested deletions
US6258571B1 (en) * 1998-04-10 2001-07-10 Genset High throughput DNA sequencing vector
WO2009052214A2 (fr) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Analyse de séquence à l'aide d'acides nucléiques décorés
WO2012050920A1 (fr) * 2010-09-29 2012-04-19 Illumina, Inc. Compositions et procédés de séquençage d'acides nucléiques

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HENIKOFF, S.: "Ordered deletions for DNA sequencing and in vitro mutagenesis by polymerase extension and exonuclease III gapping of circular templates", NUCLEIC ACIDS RESEARCH, vol. 18, 1990, pages 2961 - 2966 *
MCCOMBIE, W. R. ET AL.: "The Use of Exonuclease III Deletions in Automated DNA sequencing", METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, vol. 3, no. 1, 1991, pages 33 - 40 *
REN, Z. ET AL.: "A powerful approach for generating and sequencing DNA deletions: sequencing from outside in", ANALYTICAL BIOCHEMISTRY, vol. 245, 1997, pages 112 - 114 *
SLATKO, B. ET AL.: "Constructing nested deletions for use in DNA sequencing", CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, 1991, pages 7.2.1 - 7.2.20 *

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