WO2015092777A2 - Instrument de dispositif de remplissage de pipette semi-automatique et pipette modifiée - Google Patents

Instrument de dispositif de remplissage de pipette semi-automatique et pipette modifiée Download PDF

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Publication number
WO2015092777A2
WO2015092777A2 PCT/IB2014/067220 IB2014067220W WO2015092777A2 WO 2015092777 A2 WO2015092777 A2 WO 2015092777A2 IB 2014067220 W IB2014067220 W IB 2014067220W WO 2015092777 A2 WO2015092777 A2 WO 2015092777A2
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WO
WIPO (PCT)
Prior art keywords
pipette
plunger
barrel
filler
instrument
Prior art date
Application number
PCT/IB2014/067220
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English (en)
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WO2015092777A3 (fr
Inventor
Samrat GHOSH
Original Assignee
Indian Institute Of Science Education And Research-Mohali
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Application filed by Indian Institute Of Science Education And Research-Mohali filed Critical Indian Institute Of Science Education And Research-Mohali
Publication of WO2015092777A2 publication Critical patent/WO2015092777A2/fr
Publication of WO2015092777A3 publication Critical patent/WO2015092777A3/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps

Definitions

  • the present invention relates to a semi-automatic pipette filler instrument, which is economical, compact, lightweight, having less repetitive stress injury in handling and provided with ergonomic features.
  • the semi-automatic pipette filler (SAPF) is capable to operate not only by normal human being but also by handicapped people especially wheelchair bound.
  • SAPF semi-automatic pipette filler
  • the said modified / improved pipette instrument is having utility for teaching and research laboratories, small scale and large scale industries.
  • the conventional technique is simple and effective but use of mouth for filling pipettes could be risky if the liquid being sucked is toxic/corrosive in nature and if it inadvertently gets sucked into the mouth. In some cases, the toxic/corrosive liquid might not get into the mouth, but its vapour can cause harm to the mouth.
  • the pipette pump is available for dispensing 10 ml and 25 ml of liquid costs Rs. 450 and Rs. 650 per piece respectively.
  • the pipette controller costs Rs. 2200.
  • the cheapest commercially available manual pipette filler like the one shown in figure 1 costs approximately Rs.400 per piece. They also have to be replaced quite often as they stop working satisfactorily after a while for the following reasons:
  • Figure 39 shows a pile of dysfunctional D&N pipette fillers. Even for well-funded institutions, finding a quick replacement for defective/dysfunctional pipette fillers is not easy. Besides the usual paperwork required for ordering of expensive pipette fillers, there is considerable delay involved in procuring them even after the order has been placed. This is because local laboratory dealers do not stock such expensive imported lab wares in large numbers because of low demand, price fluctuation depending upon the strength of Indian rupee in the international market, and the limited shelf life due to natural aging of the product as mentioned earlier.
  • the primary objective of the present invention is to provide a semi-automatic pipette filler (SAPF) instrument is economical, compact, lightweight and having less repetitive stress injury in handling and provided with ergonomic features.
  • SAPF semi-automatic pipette filler
  • Yet another objective of the present invention is to provide a modified or improved pipette having less repetitive stress injury in handling and provided with suction pump.
  • Another objective of the present invention is to provide a pipette arrangement comprising a pipette (P) connected with at least one semi-automatic pipette filler (SAPF) instrument.
  • P pipette
  • SAPF semi-automatic pipette filler
  • Yet another objective of the present invention is to provide a pipette arrangement comprising a pipette (P) connected with at least one semi-automatic pipette filler (SAPF) instrument, which can be operated not only by normal human being but also by handicapped people especially wheelchair bound.
  • SAPF semi-automatic pipette filler
  • Yet another objective of the present invention is to provide a kit of semi-automatic pipette filing instrument.
  • the present invention relates to a semi-automatic pipette filler (SAPF) instrument, and an improved or modified pipette with suction pump, which is semi-automatic, economical, compact, lightweight, safety pipette filler with ergonomic features designed.
  • SAPF semi-automatic pipette filler
  • the SG-IM semi-automatic pipette filler (SAPF) has been tested for both graduated and volumetric pipettes of various sizes and can perform multipurpose tasks. It has been compared with the commercially available imported safety pipette fillers commonly used in various teaching and research laboratories in India and abroad. Handicapped people especially wheelchair bound users will find some of the designs rather convenient to use compared to commercially available ones.
  • the SG- IM SAPFs can be quickly assembled and dismantled.
  • Fig. 1 shows prior art of a two valve manual rubber pipette filler.
  • Fig. 2 depicts various parts of commercial available syringes.
  • Fig. 3 shows cross section view of rubber tubing connector according to one aspect of the present invention.
  • Fig. 4 shows SG-IM Pipette Filler Design 1 according to present invention; (b) shows D&N manual rubber pipette filler as per prior art.
  • FIG. 5 shows holding of SG-IM Pipette Filler Design I according to present invention
  • Fig. 6 exhibits dispensing of Liquid by Mode 2 using SG-IM PF Design I according to another aspect present invention.
  • Fig. 7 shows the SG-IM Pipette Filler Design II according to another aspect present invention.
  • Fig. 8 shows handling SG-IM Pipette Filler Design II according to another aspect present invention.
  • Fig. 9 depicts SG-IM Pipette Filler Design III according to another aspect present invention.
  • Fig. 10 shows holding SG-IM Pipette Filler Design III according to another aspect present invention.
  • Fig. 11 shows SG-IM Pipette Filler Design IV according to another aspect present invention.
  • Fig. 12 shows SG-IM Semi-Automatic Pipette Filler (SAPF) Design V according to another aspect present invention.
  • Fig. 13 shows shielding of rubber tubing with PVC tube according to another aspect present invention.
  • Fig. 14 exhibits dismantling of PVC tube according to another aspect present invention.
  • Fig. 15 illustrates Cross-section view of a segment of SG-IM SAPF Design VI according to another aspect present invention.
  • Fig. 16 shows SG-IM Semi-Automatic Pipette Filler (SAPF) Design VI according to another aspect present invention.
  • SAPF Pipette Filler
  • Fig. 17 shows handling of SG-IM Semi-Automatic Pipette Filler Design VI according to another aspect present invention.
  • Fig. 18 illustrates dispensing liquid by Mode 2 using SG-IM Semi-Automatic Pipette Filler (SAPF) Design VI according to another aspect present invention.
  • SAPF SG-IM Semi-Automatic Pipette Filler
  • Fig. 19 shows assembling SG-IM Manual Pipette Filler Design VII according to another aspect present invention.
  • Fig. 20 shows assembling SG-IM Manual Pipette Filler Design VII according to another aspect present invention.
  • Fig. 21 shows SG-IM Manual Pipette Filler Design VII according to another aspect present invention.
  • Fig. 22 depicts SG-IM Semi-Automatic Pipette Filler Design VIII according to another aspect present invention.
  • Fig. 23 illustrates Handling of SG-IM Semi-Automatic Pipette Filler Design VII (SAPF VII) according to another aspect present invention.
  • Fig. 24 shows SG-IM SAPF Design IX according to another aspect present invention.
  • Fig. 25 exhibits SG-IM Semi-Automatic Pipette Filler Design VIII & IX according to another aspect present invention.
  • Fig. 26 shows SG-IM Pipette Filler Design X using 20 mL Syringe according to another aspect present invention.
  • Fig. 27 shows SG-IM Universal SAPF Pipette Filler using 50mL Syringe according to another aspect present invention.
  • Fig. 28 Graduated Pipette (10ml) attached to SG-IM SAPF design VI according to another aspect present invention.
  • Fig. 29 illustrate after dismantling assembly as shown in Fig. 28.
  • Fig. 30 shows commercial Manual D&N and SG-IM Semi-Automatic Pipette Fillers according to another aspect present invention.
  • Fig. 31 (a) D&N 2- valve manual pipette filler (b) SG-IM SAPF Design XI according to another aspect present invention.
  • Fig. 32 shows holding SG-IM SAPF Design XI in Vertical Position according to another aspect present invention.
  • Fig. 33 shows holding SG-IM SAPF Design XI in Horizontal Position according to another aspect present invention.
  • Fig. 34 shows holding SG-IM Pipette Filler Design XI in Vertical Position according to another aspect present invention.
  • Fig. 35 shows holding SG-IM Pipette Filler Design XI in Horizontal Position according to another aspect present invention.
  • Fig. 36 shows SG-IM Semi-Automatic pipette Filler Design XII according to another aspect present invention.
  • Fig. 37 shows holding of SG-IM SAPF Design XII according to another aspect present invention.
  • Fig. 38 indicates position of Fixed Screws with respect to each other according to another aspect present invention.
  • Fig. 39 shows dysfunctional D&N Manual 2 valve Rubber Pipette Filler according to another aspect present invention.
  • Fig. 40 shows SG-IM Semi-Automatic pipette Filler Design XII according to another aspect present invention.
  • Fig. 41 shows SG-IM Pipette Filler Design XI in Horizontal Position according to another aspect present invention.
  • Fig. 42 shows International Symbol of Access (ISA) can be given to SG-IM Pipette Filler Design XI in Horizontal Position since its best suited for even wheelchair bound people according to another aspect present invention.
  • Fig 43 shows SG-IM Semi- Automatic Pipette Filler (SAPF) Design VI.
  • SAPF Semi- Automatic Pipette Filler
  • Fig 44 shows SG-IM Semi- Automatic Pipette Filler (SAPF) Design IX.
  • Fig 45 shows comparison of the pipette filler of the prior art and pipette instrument according to the present invention.
  • Fig 46 shows semi-automatic pipette filler (SAPF) according to the present invention.
  • Fig 46(a) shows another aspect of the present invention.
  • Fig 47 shows a pipette arrangement according to the present invention
  • Fig 47(a) shows another aspect of the present invention.
  • Fig 47 (c)-(d) shows connectors according to the present invention
  • Fig 48 shows a pipette arrangement for a person on wheel chair or of short height according to the present invention.
  • Fig 48 (a) shows another aspect of the present invention.
  • Fig 49 shows a modified pipette according to the present invention.
  • Fig 50 shows a kit of pipette filing instrument according to the present invention
  • suction pump intended to cover an assembly of plunger and suction chamber, and/or a syringe.
  • assembly of plunger and suction chamber and “syringe” are interchangeable for the understanding of invention.
  • the present invention relates to a semi-automatic pipette filler (SAPF) instrument for pipette, comprising at least one assembly of plunger (1) and suction chamber or barrel (2), wherein the assembly comprising a plunger (1) and reciprocate inside the barrel (2), the barrel having an inlet port (2i) and outlet port (2o) and at least one aperture ( 3) on the body, a threaded spacer (4) is being configured inside the barrel (2) along the plunger (1), the threaded spacer (4) is being adapted and accommodated in the space available in the plunger (1) so as to reciprocate along the plunger (1), the threaded spacer is being provided with means (5, 6) to adjust the displacement of the threaded spacer (4) and plunger (1) inside the barrel (2), a pair of safety means (7, 8) are provided with elastic members (12,13) at the outlet port (2o) of the barrel (2) that are being in contact with the plunger (1).
  • SAPF semi-automatic pipette filler
  • inlet port (2i) of barrel is preferably in the shape and size of pipette outlet (PO).
  • threaded spacer (4) is a bolt with thread and being provided with double nut system (5,6) and mounted with plunger with elastic member (9).
  • the double nut system (5,6) along with the threaded spacer assists to fill the pipette in a pre-determined manner to any meniscus level.
  • the meniscus levels is predefined and vary in the range of 1 ml to 50 ml.
  • safety means are two screw (7,8) with or without thread with rubber sleeve (12, 13) and positioned at 180° to each other at the inner side of the outlet port of barrel.
  • the safety means (7,8) prevent the plunger from being inadvertently pulled out of the barrel and loss of suction, while the user is filling any liquid in the pipette and also prevent plunger's inward movement due to the force exerted by the atmospheric pressure due to presence of partial vacuum created inside the barrel, when the plunger is pulled out by the user to fill a liquid in the pipette, thereby achieve accuracy in filling the pipette.
  • Still another aspect of the present invention relates to a pipette arrangement
  • a pipette (P) connected with at least one semi-automatic pipette filler (SAPF) instrument wherein the semi-automatic pipette filler instrument comprising at least one assembly of plunger (1) and suction chamber or barrel (2), wherein the assembly comprising a plunger (1) and reciprocate inside the barrel (2), the barrel having an inlet port (2i) and outlet port (2o) and at least one aperture ( 3) on the body, a threaded spacer (4) is being configured inside the barrel (2) along the plunger (1), the threaded spacer (4) is being adapted and accommodated in the space available in the plunger (1) so as to reciprocate along the plunger (1), the threaded spacer is being provided with means (5, 6) to adjust the displacement of the threaded spacer (4) and plunger (1) inside the barrel (2), a pair of safety means (7, 8) are provided with elastic members (12,13) at the outlet port (2o) of the barrel (2) that are being
  • pipette (P) is connected either permanently or temporarily with pipette filler instrument.
  • pipette (P) is connected permanently with pipette filler instrument (SAPF) by fusing the outlet port (PO) of pipette (P) and inlet (SAPFi) of pipette filler instrument.
  • pipette (P) is connected temporarily with pipette filler instrument by a connector (14).
  • connector (14) is selected from two way connector (20) or three way connector (21) or four way connector (22).
  • pipette is connected temporarily with pipette filler instrument by a connector (14) having first opening (14a) in accordance with outlet port (PO) of pipette (P) and second/ third /fourth openings (14b or 14c or 14d ) in accordance with inlet port (SAPFi) of pipette filler instruments.
  • a connector (14) having first opening (14a) in accordance with outlet port (PO) of pipette (P) and second/ third /fourth openings (14b or 14c or 14d ) in accordance with inlet port (SAPFi) of pipette filler instruments.
  • connector (14) for is a conduit of glass or elastomeric material.
  • connector (14) is preferably a conduit of elastomeric material.
  • the connector (14) is preferably a two way connector (14a, 14b) of elastomeric material that is having a proximal end (14a) being connected with outlet port (PO) of pipette (P) and distal end (14b) is connected to inlet port (SAPFi) of pipette filler instrument (SAPF), so as to form essentially an air tight joints.
  • connector (14) is encased with a second conduit (15) of having higher rigidity and stiffness to prevent the connector from twisting, wagging, pinching or bending during handling pipette and pipette filler instrument.
  • the second conduit (15) is made of PVC material and serrated from outside surface. Yet another aspect of the present invention, wherein the second conduit (15) is slidably adjusted on the connector (14) while assembling or disassembling the pipette and pipette filler instrument.
  • the supporting member (16) is a steel rod (17) or cable tie, being attached outside the glass pipette (P) and the pipette filler instrument (SAPF).
  • the metal clasp (19) is a ⁇ - shaped and rubber band is placed inside the a ⁇ -shaped clasp and the rubber band (10 is wound around the barrel and after desired turns, placed again in the ⁇ -shaped metal clasp.
  • a further aspect of the present invention relates to pipette arrangement for a person on wheel chair or of short height comprising a pipette (P) connected with at least two semi-automatic pipette filler (SAPF) (PFl and PF2) instrument, whereby each pipette filler instrument (PFl & PF2) having at least one assembly of plunger (1) and suction chamber or barrel (2), wherein the assembly comprising a plunger (1) and reciprocate inside the barrel (2), the barrel having an inlet port (2i) and outlet port (2o) and at least one aperture ( 3) on the body, a threaded spacer (4) is being configured inside the barrel (2) along the plunger (1), the threaded spacer (4) is being adapted and accommodated in the space available in the plunger (1) so as to reciprocate along the plunger (1), the threaded spacer is being provided with means (5,
  • first (PFl) and second (PF2) assembly of plunger & barrel are having either of same capacity or variable capacity.
  • first (PFl) and second assembly (PF2) of plunger & barrel is in the range of 1ml to 50ml.
  • a still further another aspect of the present invention relates to a modified pipette (MP) comprising an inlet port (100) and outlet port (200), whereby the outlet port (200) is a suction chamber or barrel, wherein a plunger (201) with or without grooves reciprocate inside the chamber or barrel (200), the barrel having at least one aperture (202) on the body, a threaded spacer (203) is being configured inside the barrel (200) along the plunger (201), the threaded spacer (203) is being adapted and accommodated in the space available in the plunger (201) so as to reciprocate along the plunger, the threaded spacer (203) is being provided with means (204, 205) to adjust the displacement of the threaded spacer and plunger inside the barrel, a pair of safety means (206, 207) are provided with elastic members (208, 209) on the barrel (200) that are being in contact with the plunger (201).
  • MP modified pipette
  • a still another aspect of the present invention relates to a kit of pipette filing instrument, the kit comprising a) at least one assembly of plunger and barrel (500), wherein the barrel is having inlet port, outlet port, an aperture near to inlet port and two aperture 180° near to outlet port, b) at least one threaded spacer (501) along with big nut (502) and small nut (503), c) at least a pair of safety screws (504, 505), d) at least two pair elastic members (506, 507), e) at least one connector (508) with two way opening or three way opening, f) at least one supporting member (509) in the form of steel rod or cable, g) second serrated connector (510) for encasing the first connector, h) ⁇ -shaped clasp (511), i) Bunch of Rubber bands (512), and j) One or more ring type metal clamp (513),
  • a further another aspect of the present invention relates to a method of operating the pipette filler instrument, the method comprising the steps of
  • the pipette filler is prepared from a 20ml disposable medical grade polypropylene syringe with Luer slip tip shown in figure 2.
  • the pipette filler is also prepared from a 20ml disposable medical grade polypropylene syringe with Luer slip tip shown in figure 2.
  • the syringe is a system consisting of a barrel and a plunger mounted with a gasket (rubber bung) mounted on the top end of the plunger
  • Mode 1 By just pushing the plunger in;
  • Mode 2 By removing the syringe from the female Luer stub by slight twisting as shown in figure 6;
  • Mode 3 By pushing the plunger up to certain point and then removing the syringe from the female Luer stub by slight twisting;
  • Mode 1 By just pushing the plunger in;
  • Mode 2 By removing the syringe from the female Luer slip stub by slight twisting as shown in figure 6;
  • Mode 3 By pushing the plunger up to certain point and then removing the syringe from the female Luer slip stub by slight twisting;
  • Mode 4 by just lifting the finger covering the pin hole on the barrel;
  • Mode 5 By pushing the plunger up to certain point and then lifting the finger covering the pin hole on the barrel.
  • a pair of rubber bands is used to fasten the plunger to the ears of the barrel as shown in figure 9.
  • Purpose To check whether or not instead of holding the plunger with finger, can the rubber bands wound on the barrel and the plunger prevent the plunger from small recoil (sliding in motion) due to pressure difference existing between the inside and outside of the syringe due to suction created in the syringe after pulling the plunger.
  • Rubber sleeves as shown in figure 11 are mounted loosely on the pair of screws mentioned in the previous step. Purpose: The reason is to check the following: (i) whether or not the rubber sleeves alone or along with the rubber bands can prevent the recoil of the plunger due to suction created, (ii) whether or not the rubber sleeves can prevent the plunger from being scratched by the tapered end of the screws.
  • the adjustable spacer is prepared by sawing off the head of the 9.5 cm long screw to enable inserting between the barrel and the plunger.
  • the metal disc (figure 12) has been attached to one of the ears of the barrel with a screw. It can also be attached using a 19 mm metal binder clip shown in figure 27. The purpose of the metal disc is to prevent the nut of the adjustable spacer from slipping inside the barrel when the plunger is pushed inside.
  • the modified pipette filler shown in figure 12 will be referred to as design V (24)
  • the soft rubber neck of the assembly is snugly encased in a PVC tube (7.5 cms long and 12 mm diameter) as shown in figure 13.
  • Purpose The reason is to check whether or not the PVC tube can shield the rubber neck from inadvertent pinching, twisting, wagging or bending of the soft rubber neck during handling which can cause the liquid in the pipette to eject and cause error.
  • the PVC tube is first slipped over the stem of the pipette as shown in figure 14. Then the stem is carefully inserted into one end of an inch long rubber tubing (neck) with 6mm diameter as shown in figure 14 resulting in an airtight fitting. The other end of the rubber tubing or the neck is inserted into another rubber tube ⁇ 0.5cm long (figure 14) with its outer diameter matching the inner diameter of the PVC tube.
  • the modified pipette filler shown in figure 31 will be referred to as SG-IM pipette filler design XI.
  • On one port of the 3-way adaptor is attached the stem of the pipette.
  • On another port is attached a 20 ml syringe via a female Luer slip stub.
  • On the third port is attached a 2ml syringe via a female Luer slip stub.
  • the reason is to check (i) whether or not by moving the plunger of the smaller 2 ml syringe, fine adjustment of the water level could be achieved, (ii) position of the two syringes could be swapped (figures 32 & 33) without affecting the performance which could suit some users.
  • Rubber bands are wound around both the syringe docking ports in a conventional manner.
  • the rubber band cannot be wound around in a conventional manner and therefore is wound around in a novel manner using a metal clasp as shown in figure 31.
  • Teflon tape is also used in all the three ports of the 3- way adaptor to ensure airtight fit.
  • a thin metal rod is inserted from the hole of the metal disc placed on the ear/grip of the barrel and slipped through the ring type metal clamp and rubber sleeves on the pipette stem as shown in figure 36.
  • a rubber band is tightly wound in a unique manner around the barrel and the metal rod using a ⁇ -shaped metal clasp as shown in figure 36.
  • the reason behind using the metal rod is to check whether or not the metal rod (i) can hold both the glass pipette and the pipette filler firmly together, (ii) can prevent the 3 -way rubber tubing from twisting, bending and flexing.
  • SAPF semi-automatic pipette filler
  • the syringe is connected to the pipette by an inch long rubber tubing of 6mm diameter because neither the Luer slip tip of the syringe nor the female Luer stub (figure 2) mounted on the syringe tip directly fits into the stem of the pipettes used.
  • the glass pipette and the female Luer stub inserted into both ends of the rubber tubing are air-tight.
  • mode 2 where the syringe is removed after the pipette has been filled up to the desired level, water exits the pipette up to the calibrated level (desired effect) without the need to push the plunger.
  • mode II over mode I of dispensing is that it spares the user the extra effort needed to push the plunger and manually adjust the water level at the calibrated point of the pipette.
  • mode II the user has no control over the rate of dispensing of water as the process occurs under ambient atmospheric pressure.
  • mode 3 which is basically a combination of modes 1 & 2 of dispensing, where the plunger is first pushed/ pressed up to a certain point and then the syringe is removed, water is ejected from the pipette up to the calibrated level (desired effect) without the need for the user to interfere.
  • the user can to a certain extent control the rate of dispensing of water during pushing/pressing of the plunger.
  • Dispensing by mode 2 or mode 3 from a calibrated pipette is accurate because when a pipette manufacturer calibrates the pipette, the calibration is carried out under ambient atmospheric pressure. That means after dispensing of specified volume of liquid under ambient atmospheric pressure, there will still be a small amount of liquid left behind in the pipette near its tip apparently due to capillary forces balancing the downward force exerted by the ambient atmospheric pressure. In this way, the specified volume (in this case, 20 ml) will be dispensed, and the remaining liquid should not be expelled as it will cause error in cases where accurate dispensing of specified volume of liquid is required.
  • the specified volume in this case, 20 ml
  • the dispensing process could be carried out by just one hand in comparison to design I. This whole exercise involved only pulling of the plunger to fill the pipette and the rest (water level adjustment and final dispensing) is managed through the pin hole. This further mitigated the risks of occurrence of repetitive stress injuries (RSI) as pushing of plunger is now completely obviated.
  • the pin hole allowed dispensing of water from the pipette by just one hand compared to design I where both the hands are required to dispense water by any of the three modes.
  • the PVC tube also serves the important purpose of concealing the non- obvious vital component: the 2- way rubber connector from imitation.
  • design VII (figure 21) over all earlier designs lay in the fact that due to the cable tie the syringe could not be separated/ detached from the pipette even if the plunger is pulled hard.
  • the risk of glass pipette slipping out of the assembly is very less even when compared to the commercially available pipette filler (figure 1) where there is no such clasp to hold the pipette filler and the glass pipette firmly.
  • the loop formed by the cable tie in design VII (figure 21) allows the assembly to be securely hung vertically from a wall fixture.
  • the design VII (figure 21) enables easy removal of the syringe from the assembly by just dislodging one of the strings from the ring-type metal clamps. In this manner there is no need to either cut or pry open the cable tie with a small screw driver.
  • the combination of adjustable spacer and pinhole features make the SG-IM pipette filler design VII truly semi-automatic in performance.
  • the proposed pipette filler of any design can accomplish both the task of filling pipettes and also cleaning them by strong air jet generated by pushing the plunger. Since water or any liquid can be completely expelled from the pipette due to high pressure generated by pushing in the plunger, all the designs of the pipette filler enabled cleansing of the pipette with appropriate liquid. This feature makes these designs quite distinct from the commercially available pipette filler (figure 1).
  • pipette fillers made from syringes marked 5, 10, 20 and 50 ml could precisely and accurately dispense liquids from the pipettes of capacities 5, 10, 20 and 50 ml respectively. It is possible to retract a hanging water drop by pulling the plunger. It is also observed that the size mismatch between syringe and pipette did not affect the performance as long as the capacity of the syringe used is larger than that of the pipette attached. (29) For graduated pipettes of various sizes, it is observed that the desired volume of liquid could be accurately and precisely dispensed using a 5/10/20/50 ml syringe based pipette filler. No dripping of liquid is observed.
  • a 50 ml syringe is used as universal pipette filler as shown in figure 27. All the 20ml syringe based designs (I-IX) is successfully implemented on a 50 ml syringe.
  • a 50ml syringe based pipette filler can be successfully used for filling and accurately dispensing liquid from any graduated or volumetric pipette ranging from 5-50 ml. Liquid exited the pipette as long as the plunger is pressed /pushed. As soon as the plunger is stopped, liquid too ceased to exit. It is possible to dispense liquid from the pipette drop-by-drop or as a continuous jet depending on the manner in which the plunger is pressed/ pushed.
  • the rubber bands are wound only on the plunger (figure 26) because the plunger has a V- shaped groove (figure 2) where rubber bands get stuck and prevents the plunger from moving freely if they are wound like in design III (figure 9).
  • the design XI is unique as it allows swapping of the syringe positions as shown in figures 32-35.
  • the performance of the pipette filler is not affected by interchanging the position of the syringes.
  • the other SG-IM pipette filler designs operate in vertical position only.
  • the imported pipette filler costs Rs. 400 per piece when purchased in bulk and is available only with select dealers.
  • the syringes used for making the smart pipette filler cost approximately Rs. 5, 10, 15, 25 each for 5, 10, 20 and 50 ml, respectively and are available in any chemist shop.
  • the syringes being made from polypropylene will last longer than the imported pipette fillers made from natural rubber. If some problem is suspected with the syringe, it can be easily replaced.
  • Syringes with Luer lock tip can be used in place of slip tip.
  • the present invention relates to an improved pipette instrument, which is semiautomatic, economical, compact, lightweight, safety pipette filler with ergonomic features designed and assembled from easily available components.
  • the semi-automatic pipette filler (SAPF) is capable to operate not only by normal human being but also by handicapped people especially wheelchair bound.
  • SAPF semi-automatic pipette filler
  • the improved pipette instrument is having utility for teaching and research laboratories, small scale industries.
  • the SG-IM SAPFs can be quickly assembled and dismantled for maintenance, transportation.
  • the SG-IM SAPFs is capable for precise adjustment of water level in the pipette and can be operated in either vertical or horizontal position.
  • the proposed invention is an inexpensive, rugged, light weight, compact, and user-friendly semi-automatic safety pipette filler with low risks of occurrence of repetitive stress injuries (RSI) compared to commercially available pipette filler has been developed that is performance wise precise and accurate.
  • RSI repetitive stress injuries
  • these proposed pipette fillers can dispense liquid from the pipette in five distinct modes.
  • the proposed pipette filler assembled using a 50ml capacity syringe can accurately dispense liquids from 5, 10, 20 and 50 ml volumetric pipettes and graduated pipettes. Any capacity volumetric pipette can be filled with appropriate syringe.
  • the pipette fillers of different designs allow five distinct modes of dispensing.
  • Mode 1 By just pushing the plunger in;
  • Mode 2 By removing the syringe from the female Luer stub by slight twisting;
  • Mode 3 By pushing the plunger up to certain point and then removing the syringe from the female Luer stub by slight twisting;
  • Mode 4 by just lifting the finger covering the pin hole on the barrel;
  • Mode 5 By pushing the plunger up to certain point and then lifting the finger covering the pin hole on the barrel.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention se rapporte à un instrument de dispositif de remplissage de pipette semi-automatique (SAPF pour Semi-Automatic Pipette Filler) et à une pipette perfectionnée ou modifiée comportant une pompe d'aspiration présentant des caractéristiques ou une conception ergonomiques. Le dispositif de remplissage de pipette semi-automatique (SAPF) a été testé pour des pipettes à la fois graduées et volumétriques de diverses tailles et peut effectuer des tâches polyvalentes. Il a été comparé à des dispositifs de remplissage de pipette sûrs, importés, disponibles dans le commerce et communément utilisés dans divers laboratoires d'enseignement et de recherche en Inde et à l'étranger. Les personnes handicapées, en particulier les personnes confinées dans un fauteuil roulant trouveront une grande partie des conceptions plutôt facile à utiliser par rapport à ceux disponibles dans le commerce. Les dispositifs SAPF peuvent être rapidement assemblés et démontés. La performance d'ensemble des dispositifs SAPF a été très encourageante. Puisque les pipettes sont largement utilisées dans un grand nombre de laboratoires d'éducation, de qualité de contrôle et de recherche, les entreprises de petite et de grande taille sont largement incitées à produire en masse des dispositifs de remplissage de pipette semi-automatique (SAPF).
PCT/IB2014/067220 2013-12-22 2014-12-22 Instrument de dispositif de remplissage de pipette semi-automatique et pipette modifiée WO2015092777A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN3137/DEL/2013 2013-12-22
IN3137DE2013 2013-12-22

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WO2015092777A2 true WO2015092777A2 (fr) 2015-06-25
WO2015092777A3 WO2015092777A3 (fr) 2015-11-12

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Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3827305A (en) * 1972-10-24 1974-08-06 R Gilson Adjustable pipette
US4153056A (en) * 1977-06-16 1979-05-08 Jules Silver Syringe with removable length adjusting member
GB2057291A (en) * 1979-08-31 1981-04-01 Hackett W J Safety pipette filler
US20050220676A1 (en) * 2004-04-02 2005-10-06 Tran Nathaniel T Multi-range pipette
DE102008048252A1 (de) * 2008-09-12 2010-04-15 Eppendorf Ag Pipettiervorrichtung
DE102011053808A1 (de) * 2011-09-20 2013-03-21 Cybio Ag Pipetierkopf mit Gruppen von Pipetierkanälen unterschiedlichen Durchmessers und Vorrichtung mit einem solchen Pipetierkopf

Also Published As

Publication number Publication date
WO2015092777A3 (fr) 2015-11-12

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