WO2015091139A3 - A nucleic acid analysis method and apparatus - Google Patents
A nucleic acid analysis method and apparatus Download PDFInfo
- Publication number
- WO2015091139A3 WO2015091139A3 PCT/EP2014/077185 EP2014077185W WO2015091139A3 WO 2015091139 A3 WO2015091139 A3 WO 2015091139A3 EP 2014077185 W EP2014077185 W EP 2014077185W WO 2015091139 A3 WO2015091139 A3 WO 2015091139A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- beads
- probe
- target nucleic
- sample
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/12—Measuring magnetic properties of articles or specimens of solids or fluids
- G01R33/1269—Measuring magnetic properties of articles or specimens of solids or fluids of molecules labeled with magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
Methods of detecting target nucleic acid is a sample are described. A first probe is attached to first beads, and the first beads are placed in the sample so that any target nucleic acid attaches to the first probe. A second probe also attaches to the target nucleic acid so that any of the target nucleic acid links or "tethers" the first and second probes. A capacitive sensor detects capacitance of the beads and processes capacitance data to quantify target nucleic acid presence in the sample. The second probe may be immobilised on the sensor surface. Alternatively the second beads are introduced into the sample with the second probe attached, and the extent of tethering of the first beads to the second beads is indicative of the extent of target NA present.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201480075478.9A CN105992826B (en) | 2013-12-12 | 2014-12-10 | Method for nucleic acid analysis and equipment |
US15/101,254 US10738348B2 (en) | 2013-12-12 | 2014-12-10 | Nucleic acid analysis method and apparatus |
US16/918,499 US20210024983A1 (en) | 2013-12-12 | 2020-07-01 | Nucleic acid analysis method and apparatus |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP13197025.3 | 2013-12-12 | ||
EP13197025.3A EP2883961B8 (en) | 2013-12-12 | 2013-12-12 | A nucleic acid analysis method and apparatus |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/101,254 A-371-Of-International US10738348B2 (en) | 2013-12-12 | 2014-12-10 | Nucleic acid analysis method and apparatus |
US16/918,499 Continuation US20210024983A1 (en) | 2013-12-12 | 2020-07-01 | Nucleic acid analysis method and apparatus |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2015091139A2 WO2015091139A2 (en) | 2015-06-25 |
WO2015091139A3 true WO2015091139A3 (en) | 2015-08-13 |
Family
ID=49765881
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2014/077185 WO2015091139A2 (en) | 2013-12-12 | 2014-12-10 | A nucleic acid analysis method and apparatus |
Country Status (4)
Country | Link |
---|---|
US (2) | US10738348B2 (en) |
EP (2) | EP3249055B1 (en) |
CN (1) | CN105992826B (en) |
WO (1) | WO2015091139A2 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015086654A1 (en) | 2013-12-12 | 2015-06-18 | Altra Tech Limited | A capacitive sensor and method of use |
EP3080267B1 (en) | 2013-12-12 | 2020-02-19 | Altratech Limited | A sample preparation method and apparatus |
EP3684950B1 (en) | 2017-09-20 | 2023-04-26 | Altratech Limited | Diagnostic device and system |
EP3460077B1 (en) | 2017-09-20 | 2020-06-17 | Altratech Limited | Diagnostic device and system |
CN110779967B (en) * | 2019-09-18 | 2022-06-17 | 南京农业大学 | NF-kB electrochemical detection method based on traditional glassy carbon electrode |
US20230242972A1 (en) * | 2022-01-28 | 2023-08-03 | Altratech Limited | Single-bead capacitive detector for microbiological applications |
Citations (5)
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WO1999027367A1 (en) * | 1997-11-21 | 1999-06-03 | Meinhard Knoll | Device and method for detecting analytes |
WO2001009388A1 (en) * | 1999-07-30 | 2001-02-08 | The Penn State Research Foundation | Instruments, methods and reagents for surface plasmon resonance |
WO2007106579A2 (en) * | 2006-03-15 | 2007-09-20 | Micronics, Inc. | Integrated nucleic acid assays |
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WO2011017660A2 (en) * | 2009-08-07 | 2011-02-10 | Nanomix, Inc. | Magnetic carbon nanotube based biodetection |
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-
2013
- 2013-12-12 EP EP17178871.4A patent/EP3249055B1/en active Active
- 2013-12-12 EP EP13197025.3A patent/EP2883961B8/en active Active
-
2014
- 2014-12-10 US US15/101,254 patent/US10738348B2/en active Active
- 2014-12-10 WO PCT/EP2014/077185 patent/WO2015091139A2/en active Application Filing
- 2014-12-10 CN CN201480075478.9A patent/CN105992826B/en active Active
-
2020
- 2020-07-01 US US16/918,499 patent/US20210024983A1/en active Pending
Patent Citations (5)
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WO1999027367A1 (en) * | 1997-11-21 | 1999-06-03 | Meinhard Knoll | Device and method for detecting analytes |
WO2001009388A1 (en) * | 1999-07-30 | 2001-02-08 | The Penn State Research Foundation | Instruments, methods and reagents for surface plasmon resonance |
WO2007106579A2 (en) * | 2006-03-15 | 2007-09-20 | Micronics, Inc. | Integrated nucleic acid assays |
US20100267162A1 (en) * | 2009-04-16 | 2010-10-21 | Kartalov Emil P | Methods for quantitative target detection and related devices and systems |
WO2011017660A2 (en) * | 2009-08-07 | 2011-02-10 | Nanomix, Inc. | Magnetic carbon nanotube based biodetection |
Non-Patent Citations (1)
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Also Published As
Publication number | Publication date |
---|---|
EP2883961A1 (en) | 2015-06-17 |
CN105992826A (en) | 2016-10-05 |
EP2883961B8 (en) | 2017-09-27 |
WO2015091139A2 (en) | 2015-06-25 |
EP3249055B1 (en) | 2019-06-26 |
US20160304941A1 (en) | 2016-10-20 |
EP2883961B1 (en) | 2017-08-09 |
EP3249055A1 (en) | 2017-11-29 |
US20210024983A1 (en) | 2021-01-28 |
CN105992826B (en) | 2019-08-30 |
US10738348B2 (en) | 2020-08-11 |
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