WO2015089055A1 - Utilisation d'inositols et d'inositols à substitution phosphate comme termiticides - Google Patents

Utilisation d'inositols et d'inositols à substitution phosphate comme termiticides Download PDF

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Publication number
WO2015089055A1
WO2015089055A1 PCT/US2014/069319 US2014069319W WO2015089055A1 WO 2015089055 A1 WO2015089055 A1 WO 2015089055A1 US 2014069319 W US2014069319 W US 2014069319W WO 2015089055 A1 WO2015089055 A1 WO 2015089055A1
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WIPO (PCT)
Prior art keywords
inositol
cellulose
composition according
termites
cycloaliphatic
Prior art date
Application number
PCT/US2014/069319
Other languages
English (en)
Inventor
Roger A. Laine
Lucas James VEILLON
Gregg Henderson
Original Assignee
Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College filed Critical Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College
Publication of WO2015089055A1 publication Critical patent/WO2015089055A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
    • A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/04—Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals

Definitions

  • the present invention relates to a novel composition for controlling termites without the use of chemicals toxic to mammals.
  • Myo- inositol, phytic acid (also known as phytate), and related compounds when combined separately with a food source, for example, cellulose, are toxic to termites.
  • yo-inositol and phytic acid without a food source do not appear to be toxic to termites.
  • rnyo-inositol and phytic acid are non-toxic to mammals.
  • C. formosanus is the most destructive and economically important worldwide.
  • C. formosanus infestations damage a wide variety of wooden structures, as well as over 50 species of living plants. They also cause damage to non- cellulosic materials such as insulation on buried electrical and telephone wires.
  • the first general survey of an exotic C. formosanus infested region in the continental US was conducted in Louisiana in 1966, and since that time, the US populations of C. formosanus have grown rapidly. A study of this species in New Louisiana, Louisiana showed that termite alates increased by a factor of fourteen over a seven year period.
  • C. formosanus digest their wood diets by utilizing exogenous endo- -3 ,4- glucanase (cellulose) and ⁇ -glucosidase (cellobiase) enzymes secreted into phagocytotic lumens by symbiotic hindgut protozoa that are crucial in cellulose and lignin metabolism. They also have endogenous endo-p-l ,4-glucanase and ⁇ -glucosidase enzymes expressed in the salivary glands and midgut.
  • Pseudotrichonympha grassii Koidzumi Holomastigotoides hartmanni Koidzumi and Spirotrichonymph leidyi Koidzumi comprise the obligatory C. formosanus hindgut protozoan population, which themselves have obligatory intracellular bacterial symbionts. Coexisting with these organisms are numerous species of bacteria involved in methanogenesis, acetogenesis, nitrogen fixation, sulfate reduction and oxygen scavenging that combine to form a complex and remarkable ancient micro-ecosystem.
  • Guadalupe, et al. (U.S. Patent Number 6,585,991) teaches the use of termite baits that comprise foods that attract termites along with termite poisons such as hexaflumuron, imisacloprin, fipronil, or other bioactive compounds.
  • the inventions disclosed herein are novel method and compositions that are not harmful to mammals, and in particular, not harmful to humans, while exhibiting excellent toxicity towards termites. Further, this novel composition is particularly effective against Formosan subterranean termites, Coptotermes formosanus Shiraki.
  • This composition comprises a mixture of a food source, for example, cellulose, and an environmentally compatible chemical, for example, various forms of Inositol and Phytate.
  • myoinositol and phytate myo-inositol-l , 2,3,4,5, 6-hexaAv.sphosphate, also known as phytic acid
  • myoinositol and phytate myo-inositol-l , 2,3,4,5, 6-hexaAv.sphosphate, also known as phytic acid
  • inositol There are nine isomeric forms of inositol (cyclohexanol), but the myo- is the most common form. (See Figure 3). At least five other isomeric forms of inositol have been isolated, including chiro-, epi-, m co-, mo- and sc / oinositofs. Inositols, in general, and myo-inositoi, in particular, are non-toxic to humans, and in fact, wyo-inositol is sold as a dietary supplement for humans.
  • Phosphorylated myoinositols are also common. The most abundant of these species is w ⁇ o-inositol-l,2,3,4,5,6-hexaA:0'phosphate, also known as phytic acid or phytate (See Figure 2). Phytate is accumulated in plant organs and tissues, including tubers, turions, roots and pollen, and is the principal form of phosphorus storage in seeds. Phytate is ubiquitous in eukaryotes. It is usually the most plentiful inositol derivative in cells where it, and other phosphorylated myoinositol derivatives, is involved in numerous functions outside nutrient storage.
  • Figure 1 Chemical structure of myoinositol.
  • Figure 2 Chemical structure of phytate.
  • Figure 4 Percent mortality of C. formosanus following treatment of food source filter paper with myoinositol or phytate. DETAILED DESCRIPTION OF THE INVENTION
  • yo-inositol and phytate are toxic to Formosan subterranean termites only when the termites were consuming a food, such as cellulose, without the need to add another toxic compound to the mixture.
  • Myoinositol significantly increased mortality when applied to food source filter paper discs at 640.8 1281 ,7 ⁇ g mm 3 and 1281 .7 ⁇ g/mm 3 in three independent bioassays ( Figures 3 and 4).
  • C. formosanus were destroyed following treatment of food source filter paper with myoinositol in no-choice assays with twenty workers. Several separate collections were used and the data were recorded daily for fourteen days. We evaluated the effectiveness of myoinositol at the following concentrations of 160.2 ⁇ g/mm 3 , 320,4 g/mm 3 , 640.8 ⁇ g/mm 3 , and 1281.7 g/mm 3 . In addition, we examined the C. formosamis hindgut protozoan counts of workers for fourteen days while termites were allowed to feed on filter paper treated with 1281.7 ⁇ g/mm 3 of myo-inositol.
  • Termite-containing crates were held in 250-liter plastic garbage cans kept at room temperature (26 -28 °C) and 70 - 80% relative humidity with the original food source.
  • Collection group E was used in the myoinositol 1281.7, 640.8, 320.4 and 160.2 ⁇ g mm 3 dose mortality assay.
  • 640.8 and 1281.7 ⁇ g/mm 3 treatment levels some variation was observed. Specifically, at the 640.8 ⁇ / ⁇ 3 dosage, mortality in group C was significantly different from groups E and C on days four, five, and six.
  • Test compounds were applied to 42.5 mm filter papers, in plastic Petri dishes measuring 60x15x5 mm.
  • 1 mg of carbohydrate per 10 ⁇ of distilled water (dH 2 0) was the stock solution using 2.5 mg (160.2 ⁇ g/mm 3 ) of filter paper, 5 mg (320.4 ⁇ g/mm 3 ), 10 mg (640.8 ⁇ g/mm 3 ) and 20 mg (1281 .7 ⁇ g/mm 3 ) of wyo-inositol.
  • Termite mortality was recorded daily for fourteen days in triplicate experiments.
  • a one-way analysis of variance (ANOVA) performed using SAS/STAT® software (version 9.1), followed by Tukey's studentized range test was used to evaluate statistical differences among groups (SAS Institute 2002). All mortality data were judged at a - 0.05.
  • Termites from collection group C were used in both the myo-inositol 1281.7, 640.8 and 320.4 ⁇ g/mm 3 dose mortality assay and the wyo-inositol and phytate 1281.7, 640.8 and 320.4 ⁇ g mm 3 dose mortality assay, and termites from collection group E were used in the myo-inositol 1281.7, 640.8, 320.4 and 160.2 ⁇ g/mm 3 dose mortality assay.
  • Hindguts were removed from three workers and gently macerated in 40 ⁇ of saline solution containing neutral red dye (0.5 mL of 1% aqueous neutral red solution dissolved in 10 mL saline solution).
  • the number of each protozoan species was determined with a hemocytometer under a light microscope.
  • Myo ' inositol bioassay Test one for filter paper treated with 1281.7 ⁇ g/mm 3 of myoinositol on total protozoan populations showed significance on day three for P, grassii, and reduced populations on days four, five and eight. H. hartmanni and S. leidyi, showed significance on days four through eight, and reduced populations on days eleven and thirteen, respectively.
  • Artificial diets were prepared by dissolving 400 mg/10 mL of selected sugars in 150 mg/10 mL of agar in distilled water with a pH of 6.8. Sugar and agar solutions were autoclaved separately. Agar-sugar solutions were poured into polystyrene Petri dishes measuring 60x15x5 mm, allowed to solidify, and then divided into quadrants.
  • Afyo- inositol, 2 glucose, 2-deoxy-Z> ⁇ galactose and ⁇ -galactose were all examined as carbon sources along with a control of unsupplemented agar.
  • twenty worker termites from collection group B for the myoinositol and D- glucose (40mg/mL agar) assay, or collection group D for the myoinositol, -glucose, D- galactose, 2-deoxy-Z>-galactose (40mg/mL agar) and agar alone assay again were placed in polystyrene Petri dishes measuring 60x15x5 mm and provided with an agar-sugar quadrant to serve as a food and water source.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un nouveau termiticide comprenant un phénol cyclique, par exemple l'inositol, ou un dérivé phosphate d'un phénol cyclique, par exemple un phytate, associé avec la cellulose, et un procédé d'utilisation de ce nouveau termiticide.
PCT/US2014/069319 2013-12-11 2014-12-09 Utilisation d'inositols et d'inositols à substitution phosphate comme termiticides WO2015089055A1 (fr)

Applications Claiming Priority (2)

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US201361914628P 2013-12-11 2013-12-11
US61/914,628 2013-12-11

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WO2015089055A1 true WO2015089055A1 (fr) 2015-06-18

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000062610A1 (fr) * 1999-04-20 2000-10-26 The United States Of America, As Represented By The Secretary Of Agriculture Matrice d'appats pour termites
US6413551B1 (en) 1998-07-30 2002-07-02 Douglas Mervyn Gray Termiticide composition
US20020172658A1 (en) * 2001-03-05 2002-11-21 Brode Philip Frederick Devices and methods for eliminating termite colonies
EP1585389A2 (fr) 2003-01-23 2005-10-19 Fmc Corporation Compositions termiticides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6413551B1 (en) 1998-07-30 2002-07-02 Douglas Mervyn Gray Termiticide composition
WO2000062610A1 (fr) * 1999-04-20 2000-10-26 The United States Of America, As Represented By The Secretary Of Agriculture Matrice d'appats pour termites
US6585991B1 (en) 1999-04-20 2003-07-01 The United States Of America As Represented By The Secretary Of Agriculture Termite bait matrix
US20020172658A1 (en) * 2001-03-05 2002-11-21 Brode Philip Frederick Devices and methods for eliminating termite colonies
EP1585389A2 (fr) 2003-01-23 2005-10-19 Fmc Corporation Compositions termiticides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JUDITH REINHARD: "Thin-Layer Chromatography assessing feeding stimulation by labial gland secretion compared to synthetic chemicals in the subterranean termite Reticulitermes santonensis", JOURNAL OF CHEMICAL ECOLOGY, vol. 27, no. 1, 2001, pages 175 - 187, XP002735254 *
VEILLON LUCAS JAMES: "The Biological Activity of rare carbohydrates and cyclitols in coptotermes formosanus", 27 October 2011 (2011-10-27), XP002735188, Retrieved from the Internet <URL:http://etd.lsu.edu/docs/available/etd-11082011-160845/unrestricted/LucasVeillon.Dissertation.pdf> [retrieved on 20150129] *

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