WO2015085193A1 - Methods useful in the synthesis of halichondrin b analogs - Google Patents
Methods useful in the synthesis of halichondrin b analogs Download PDFInfo
- Publication number
- WO2015085193A1 WO2015085193A1 PCT/US2014/068834 US2014068834W WO2015085193A1 WO 2015085193 A1 WO2015085193 A1 WO 2015085193A1 US 2014068834 W US2014068834 W US 2014068834W WO 2015085193 A1 WO2015085193 A1 WO 2015085193A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- eribulin
- pharmaceutically acceptable
- acceptable salt
- produce
- salt
- Prior art date
Links
- 0 CC(CC(CCCOC)OC1CC(C2CC(CC(CC3)OC4[C@]3OCC[C@]4*)=O)OC(CC(*)CO)[C@@]2OC)C1=C Chemical compound CC(CC(CCCOC)OC1CC(C2CC(CC(CC3)OC4[C@]3OCC[C@]4*)=O)OC(CC(*)CO)[C@@]2OC)C1=C 0.000 description 6
- DRIHUUFRFITXNV-OTGGJCDQSA-N C[C@H](CC(CC[C@@H](C(C1)=C)OC1CCC(C[C@H](C([C@@H]1OC(CC2)[C@@H]3OC2CC(CC24)=O)O)OC1C3O)=O)OC1CC2OC(CC(CO)O)[C@@H]4OC)C1=C Chemical compound C[C@H](CC(CC[C@@H](C(C1)=C)OC1CCC(C[C@H](C([C@@H]1OC(CC2)[C@@H]3OC2CC(CC24)=O)O)OC1C3O)=O)OC1CC2OC(CC(CO)O)[C@@H]4OC)C1=C DRIHUUFRFITXNV-OTGGJCDQSA-N 0.000 description 1
- IUKSWGKZBHVYPQ-IZIYGDMISA-O C[C@H](CC(CC[C@@H](C(C1)=C)OC1CC[C@](C[C@H]1[OH2+])(OC1[C@@H](C1)OC2CC3)OC1[C@H]2OC3CC(CC12)=O)OC3C[C@@H]1OC(CC(CN)O)[C@@H]2OC)C3=C Chemical compound C[C@H](CC(CC[C@@H](C(C1)=C)OC1CC[C@](C[C@H]1[OH2+])(OC1[C@@H](C1)OC2CC3)OC1[C@H]2OC3CC(CC12)=O)OC3C[C@@H]1OC(CC(CN)O)[C@@H]2OC)C3=C IUKSWGKZBHVYPQ-IZIYGDMISA-O 0.000 description 1
- YSFVUMFJVUORRK-YPJPRJBRSA-N C[C@H](C[C@H](CCC(C(C1)=C)OC1CCC(C[C@H](C1O)OC(CO)[C@H]1OC(CC1)COC1CC(CC12)=O)=O)OC3C[C@@H]1OC(CC(CO)O)[C@@H]2OC)C3=C Chemical compound C[C@H](C[C@H](CCC(C(C1)=C)OC1CCC(C[C@H](C1O)OC(CO)[C@H]1OC(CC1)COC1CC(CC12)=O)=O)OC3C[C@@H]1OC(CC(CO)O)[C@@H]2OC)C3=C YSFVUMFJVUORRK-YPJPRJBRSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/22—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains four or more hetero rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
Definitions
- the invention relates to methods useful in the synthesis of analogs of halichondrin B, in particular ER-086526, referred to under its generic name eribulin throughout the following specification.
- Eribulin (marketed under the trade name HALAVEN® as eribulin mesylate), a nontaxane microtubule dynamics inhibitor, is a structurally simplified, synthetic analog of the marine natural product halichondrin B.
- HALAVEN® eribulin mesylate
- Methods for the synthesis of eribulin and other halichondrin B analogs are described in U.S. Patent Nos. 6,214,865, 6,365,759, 6,469,182, 7,982,060, and 8,148,554, the syntheses of which are incorporated herein by reference. New methods for the synthesis of halichondrin B analogs, in particular eribulin and eribulin mesylate, are desirable.
- the present invention features improved methods useful for the synthesis of analogs of halichondrin B, such as eribulin and pharmaceutically acceptable salts thereof (e.g., eribulin mesylate).
- the invention features a method of preparing an intermediate in the synthesis of eribulin including reacting a compound having formula (I) :
- each of R 1 , R 2 , R 3 , R 4 , and R 5 is independently a silyl group (e.g., trimethylsilyl (TMS), triethylsilyl (TES), f-butyldimethylsilyl (TBS), f-butyldiphenylsilyl (TBDPS), triisopropylsilyl (TIPS), or triphenylsilyl (TPS)), with a fluoride source (e.g., tetrabutylammonium fluoride) in a solvent including an amide, e.g., a A/,/ C1 -C6 dialkyl C1 -C6 alkyl amide or A/ C1 -C6 alkyl C2-C6 lactam, such as A/,A/-dimethylacetamide (e.g., as a mixture of tetrahydrofuran and A/,A/-dimethylacetamide), A/,
- ER-81 1475 may be produced in a mixture with its C12 stereoisomer, ER-81 1474:
- the method may further include adding a mixture of acetonitrile and water to increase the yield of ER- 81 1475.
- each of R 1 , R 2 , R 3 , R 4 , and R 5 is f-butyldimethylsilyl (TBS).
- the invention further features a method of preparing an intermediate in the synthesis of eribulin including reacting (e.g., in ethanol) ER-81 1475 with a conjugate acid of imidazole (e.g., imidazole hydrochloride) to produce the intermediate ER-076349:
- ER-81 1475 can be produced by any of the methods provided herein.
- the invention features a method of preparing an intermediate in the synthesis of eribulin.
- This method includes reacting (e.g., in acetonitrile) ER-076349 with a sulfonylating reagent, e.g., tosyl chloride, in the presence of a metal catalyst (e.g., dibutyltin oxide) to produce the intermediate:
- a sulfonylating reagent e.g., tosyl chloride
- a metal catalyst e.g., dibutyltin oxide
- R 6 is sulfonyl, e.g.,ER-082892.
- the reacting may occur above 0°C.
- ER-076349 can be produced by any of the methods provided herein.
- the methods may also include addition of a base, e.g., a C1 -6 trialkylamine, such as triethylamine or A/,A/-diisopropylethylamine.
- the invention also features a method of producing eribulin.
- This method includes producing intermediate ER-81 1475 by any one of the foregoing methods, ketalizing ER-81 1475 to produce the intermediate ER-076349, and aminating ER-076349 to produce eribulin (ER-086526) :
- the step of ketalizing ER-81 1475 may include converting ER-81 1475 to ER-076349 according to any of the methods provided herein.
- the step of aminating ER-076349 to produce eribulin may include converting ER-076349 to ER-082892 according to any of the methods provided herein.
- the invention further features an alternative method of producing eribulin.
- This method includes producing intermediate ER-076349 by any one of the foregoing methods and aminating ER-076349 to produce eribulin.
- the step of aminating ER-076349 to produce eribulin may include converting ER- 076349 to ER-082892 according to any of the methods provided herein.
- the invention features yet another method of producing eribulin.
- This method includes producing intermediate ER-082892 by any of the methods provided herein and aminating ER- 082892 to produce eribulin.
- Any method of producing eribulin may further include salifying eribulin to produce a
- eribulin e.g., eribulin mesylate
- the invention further features a method of manufacturing a pharmaceutical product including eribulin or a pharmaceutically acceptable salt thereof (e.g., eribulin mesylate).
- This method includes producing or directing the production of eribulin or a pharmaceutically acceptable salt thereof by any one of the foregoing methods and processing or directing the processing of eribulin or a pharmaceutically acceptable salt thereof into a pharmaceutical product including eribulin or a pharmaceutically acceptable salt thereof, thereby manufacturing a pharmaceutical product including eribulin or a pharmaceutically acceptable salt thereof.
- the processing step can include one or more of formulating eribulin or a pharmaceutically acceptable salt thereof (e.g., eribulin mesylate) ; processing eribulin or a pharmaceutically acceptable salt thereof into a drug product; combining eribulin or a pharmaceutically acceptable salt thereof with a second component (e.g., an excipient or pharmaceutically acceptable carrier) ; lyophilizing eribulin or a pharmaceutically acceptable salt thereof; combining a first and second batch of eribulin or a
- a second component e.g., an excipient or pharmaceutically acceptable carrier
- eribulin or a pharmaceutically acceptable salt thereof to provide a third larger batch; disposing eribulin or a pharmaceutically acceptable salt thereof into a container (e.g., a gas or liquid tight container) ; packaging eribulin or a pharmaceutically acceptable salt thereof; associating a container including eribulin or a pharmaceutically acceptable salt thereof with a label; and shipping or moving eribulin or a
- a container e.g., a gas or liquid tight container
- a number following an atomic symbol indicates the total number of atoms of that element that are present in a particular chemical moiety.
- other atoms such as hydrogen atoms, or substituent groups, as described herein, may be present, as necessary, to satisfy the valences of the atoms.
- an unsubstituted C2 alkyl group has the formula -CH 2 CH 3 .
- a reference to the number of oxygen, nitrogen, or sulfur atoms in a heteroaryl group only includes those atoms that form a part of a heterocyclic ring.
- alkyl is meant a straight or branched chain saturated cyclic (i.e., cycloalkyl) or acyclic hydrocarbon group of from 1 to 12 carbons, unless otherwise specified.
- exemplary alkyl groups include C1 -C8, C1 -C6, C1 -C4, C2-C7, C3-C12, and C3-C6 alkyl.
- alkyl groups used in any context herein, are optionally substituted with halogen, alkoxy, aryloxy, arylalkyloxy, oxo, alkylthio, alkylenedithio, alkylamino,
- alkylamino is meant -NHR, wherein R is alkyl.
- alkenyljalkylamino is meant -NRR', wherein R is alkyl, and R' is alkenyl.
- aryljalkylamino is meant -NRR', wherein R is alkyl, and R' is aryl.
- [arylalkyljalkylamino” is meant -NRR', wherein R is alkyl, and R' is arylalkyl.
- dialkylamino is meant -NR 2 , wherein each R is alkyl, selected independently.
- alkylene is meant a divalent alkyl group.
- Alkylene groups used in any context herein, are optionally substituted in the same manner as alkyl groups.
- an unsubstituted C1 alkylene group is -CH 2 -.
- alkylenedithio is meant -S-alkylene-S-.
- alkylthio is meant -SR, wherein R is alkyl.
- alkenyl is meant a straight or branched chain cyclic or acyclic hydrocarbon group of, unless otherwise specified, from 2 to 12 carbons and containing one or more carbon-carbon double bonds.
- alkenyl groups include C2-C8, C2-C7, C2-C6, C2-C4, C3-C12, and C3-C6 alkenyl. Specific examples include ethenyl (i.e., vinyl), 1 -propenyl, 2-propenyl (i.e., allyl), 2-methyl-1 -propenyl, 1 -butenyl, 2- butenyl (i.e., crotyl), and the like.
- Alkenyl groups, used in any context herein are optionally substituted in the same manner as alkyl groups. Alkenyl groups, used in any context herein, may also be substituted with an aryl group.
- alkoxy is meant -OR, wherein R is alkyl.
- aryl is meant a monocyclic or multicyclic ring system having one or more aromatic rings, wherein the ring system is carbocyclic or heterocyclic.
- Heterocyclic aryl groups are also referred to as heteroaryl groups.
- a heteroaryl group includes 1 to 4 atoms selected independently from 0, N, and S.
- Exemplary carbocyclic aryl groups include C6-C20, C6-C15, C6-C10, C8-C20, and C8-C15 aryl.
- a preferred aryl group is a C6-10 aryl group.
- carbocyclic aryl groups include phenyl, indanyl, indenyl, naphthyl, phenanthryl, anthracyl, and fluorenyl.
- exemplary heteroaryl groups include monocylic rings having from 1 to 4 heteroatoms selected independently from 0, N, and S and from 1 to 6 carbons (e.g., C1 -C6, C1 -C4, and C2-C6).
- Monocyclic heteroaryl groups preferably include from 5 to 9 ring members.
- Other heteroaryl groups preferably include from 4 to 19 carbon atoms (e.g., C4-C1 0).
- heteroaryl groups include pyridinyl, quinolinyl, dihydroquinolinyl, isoquinolinyl, quinazolinyl, dihydroquinazolyl, and tetrahydroquinazolyl.
- aryl groups used in any context herein, are optionally substituted with alkyl, alkenyl, aryl, arylalkyi, halogen, alkoxy, aryloxy, arylalkyloxy, oxo, alkylthio, alkylenedithio, alkylamino, [alkenyljalkylamino, [aryljalkylamino,
- arylalkyi is meant -R'R", wherein R' is alkylene, and R" is aryl.
- arylalkyloxy is meant -OR, wherein R is arylalkyi.
- aryloxy is meant -OR, wherein R is aryl.
- carboxyl is meant -C(0)OH, in free acid, ionized, or salt form.
- fluoride source is meant a compound that can be a source of soluble fluoride ion (i.e., F " ) (e.g., to remove silyl ether hydroxyl protecting groups)
- exemplary fluoride sources include, ammonium fluoride, benzyltriethylammonium fluoride, cesium fluoride (i.e., CsF), 1 -chloromethyl-4-fluoro-1 ,4- diazoniabicyclo[2.2.2]octane bis(tetrafluoroborate) (i.e., Selectfluor®), hydrofluoric acid (i.e., HF), poly[4- vinylpyridinium poly(hydrogen fluoride)], potassium fluoride (i.e., KF), pyridine hydrogen fluoride (i.e., HF- pyridine), sodium fluoride (i.e., NaF), tetrabutylammonium fluoride (i.e., TB
- halogen is meant fluoro, chloro, bromo, or iodo.
- lactam is meant a cyclic amide, wherein the ring consists of carbon atoms and one nitrogen atom.
- leaving group is meant a group that is displaced during a chemical reaction. Suitable leaving groups are well known in the art, e.g., see, Advanced Organic Chemistry, March, 4th Ed., pp. 351 -357, John Wiley and Sons, N .Y. (1992).
- Such leaving groups include halogen, C1 -C12 alkoxy (e.g., C1 -C8, C1 -C6, C1 -C4, C2-C7, and C3-C6 alkoxy), C1 -C12 alkylsulfonate (e.g., C1 -C8, C1 -C6, C1 -C4, C2-C7, C3-C12, and C3-C6 alkylsulfonate), C2-C12 alkenylsulfonate (e.g., C2-C8, C2-C6, C2-C4, C3-C12, and C3-C6 alkenylsulfonate), carbocyclic C6-C20 arylsulfonate (e.g., C6-C15, C6-C10, C8-C20, and C8-C15 arylsulfonate), C4-C1 9 heteroarylsulfonate (e.g., C4-C10
- Alkylsulfonates, alkenylsulfonates, arylsulfonates, heteroarylsulfonates, arylalkylsulfonates, and heteroarylalkylsulfonates can be optionally substituted with halogen (e.g., chloro, iodo, bromo, or fluoro), alkoxy (e.g., C1 -C6 alkoxy), aryloxy (e.g., C6-C1 5 aryloxy, C4-C19 heteroaryloxy, and C1 -C6
- halogen e.g., chloro, iodo, bromo, or fluoro
- alkoxy e.g., C1 -C6 alkoxy
- aryloxy e.g., C6-C1 5 aryloxy, C4-C19 heteroaryloxy, and C1 -C6
- heteroaryloxy oxo, alkylthio (e.g., C1 -C6 alkylthio) , alkylenedithio (e.g., C1 -C6 alkylenedithio), alkylamino (e.g., C1 -C6 alkylamino), [alkenyl]alkylamino (e.g., [(C2-C6)alkenyl](C1 -C6)alkylamino),
- [aryl]alkylamino e.g., [(C6-C10)aryl](C1 -C6)alkylamino, [(C1 -C6)heteroaryl](C1 -C6)alkylamino, and [(C4- C19)heteroaryl](C1 -C6)alkylamino
- [arylalkyl]alkylamino e.g., [(C6-C1 0)aryl(C1 -C6)alkyl](C1 - C6)alkylamino, [(C1 -C6)heteroaryl(C1 -C6)alkyl](C1 -C6)alkylamino, [(C4-C1 9)heteroaryl(C1 -C6)alkyl](C1 - C6)alkylamino), dialkylamino (e.g., di(C1 -C6 alkyl)amino), silyl (
- Alkenylsulfonates can be optionally substituted with carbocyclic aryl (e.g., C6-C15 aryl) , monocyclic C1 -C6 heteroaryl, or C4-C19 heteroaryl (e.g., C4- C10 heteroaryl).
- Arylsulfonates can be optionally substituted with alkyl (e.g., C1 -C6 alkyl) or alkenyl (e.g., C2-C6 alkenyl).
- any heteroaryl group present in a leaving group has from 1 to 4 heteroatoms selected independently from 0, N, and S.
- Suitable leaving groups include chloro, iodo, bromo, fluoro, methanesulfonate (mesylate), 4-toluenesulfonate (tosylate), trifluoromethanesulfonate (triflate, OTf), nitro-phenylsulfonate (nosylate), and bromo-phenylsulfonate (brosylate). Leaving groups may also be further substituted as is known in the art.
- pharmaceutically acceptable salt is meant a salt within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and animals without undue toxicity, irritation, allergic response and the like and commensurate with a reasonable benefit/risk ratio.
- Pharmaceutically acceptable salts are well known in the art. For example, pharmaceutically acceptable salts are described in Berge et al., J. Pharmaceutical Sciences 66:1 -19, 1 977 and Pharmaceutical Salts: Properties,
- Representative acid addition salts include acetate, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fumarate, glucoheptonate, glycerophosphate, hemisulfate, heptonate, hexanoate, hydrobromide (i.e., HBr), hydrochloride (i.e., HCI), hydroiodide (i.e., H I), 2-hydroxy- ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate,
- methanesulfonate i.e., mesylate
- 2-naphthalenesulfonate nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, toluenesulfonate (i.e., tosylate), undecanoate, valerate salts and the like.
- ilyl is meant -SiR 3 , wherein each R is independently alkyl, alkenyl, aryl, or arylalkyl.
- silyl groups include tri(C1 -C6 alkyl)silyl, tri(C6-C10 aryl or C1 -C6 heteroaryl)silyl, di(C6-C10 aryl or C1 -C6 heteroaryl)(C1 -C6 alkyl)silyl, and (C6-C1 0 aryl or C1 -C6 heteroaryl)di(C1 -C6 alkyl)silyl. It will be understood that, when a silyl group includes two or more alkyl, alkenyl, aryl, heteroaryl, or arylalkyl groups, these groups are independently selected.
- any heteroaryl group present in a silyl group has from 1 to 4 heteroatoms selected independently from 0, N, and S.
- Silyl groups are known in the art, e.g., as described in Greene's Protective Groups in Organic Synthesis, Wiley-lnterscience, 4 Edition, 2006. Specific examples of silyl groups include trimethylsilyl (TMS), triethylsilyl (TES), f-butyldimethylsilyl (TBS), f-butyldiphenylsilyl (TBDPS), triisopropylsilyl (TIPS), and triphenylsilyl (TPS) ethers.
- Silyl groups may be substituted as is known in the art; for example, aryl and arylalkyl groups, such as phenyl, benzyl, naphthyl, or pyridinyl, can be substituted with C1 -C6 alkyl, C1 -C6 alkoxy, nitro, cyano, carboxyl, or halogen.
- aryl and arylalkyl groups such as phenyl, benzyl, naphthyl, or pyridinyl
- Alkyl groups such as methyl, ethyl, isopropyl, n-propyl, f-butyl, n-butyl, and sec-butyl, and alkenyl groups, such as vinyl and allyl, can also be substituted with oxo, arylsulfonyl, halogen, and trialkylsilyl groups.
- sulfonyl is meant -S(0) 2 R, wherein R is alkyl, alkenyl, aryl, arylalkyl, or silyl.
- R is C1 -C12 alkyl (e.g., C1 -C8, C1 -C6, C1 -C4, C2-C7, C3-C12, and C3-C6 alkyl), C2- C12 alkenyl (e.g., C2-C8, C2-C6, C2-C4, C3-C12, and C3-C6 alkenyl), carbocyclic C6-C20 aryl (e.g., C6- C15, C6-C10, C8-C20, and C8-C15 aryl), monocyclic C1 -C6 heteroaryl (e.g., C1 -C4 and C2-C6 heteroaryl), C4-C1 9 heteroaryl (e.g., C4-
- Exemplary sulfonyl groups include tosyl, triflyl, and mesyl.
- the present invention provides methods for the synthesis of halichondrin B analogs.
- the methods are useful for the synthesis of eribulin and pharmaceutically acceptable salts thereof:
- the C14-C35 portion (e.g., ER-804028) of the molecule is coupled to the C1 -C13 portion (e.g., ER-803896) to produce the C1 -C35 acyclic intermediate (e.g., ER-804029), and additional reactions are carried out to produce a compound of formula (I) (e.g., ER-1 18046) as shown in Scheme 1 :
- deprotonation e.g., by lithiation
- C14-C35 sulfone fragment i.e., ER-804028
- C1 -C13 aldehyde fragment i.e., ER-803896
- ER-804029 a mixture of diastereomeric alcohols
- Step A Conversion of a compound of formula (I) to ER-811475
- a fluoride source e.g., tetrabutylammonium fluoride
- a conjugate acid of imidazole e.g., imidazole hydrochloride
- Method A2 Deprotection with Fluoride Source in an Amide, e.g., DMAC
- a fluoride source e.g., tetrabutylammonium fluoride
- a conjugate acid of imidazole e.g., imidazole hydrochloride
- an amide e.g., A/,A/-dimethylacetamide (DMAC)
- DMAC a conjugate acid of imidazole
- solvent e.g., a mixture of tetrahyrofuran (THF) and DMAC
- amides include an A/,/ C1 -C6 dialkyl C1 -C6 alkyl amide or A/ C1 -C6 alkyl C2-C6 lactam, such as ⁇ , ⁇ -dimethylformamide, N-methyl 2- pyrrolidone, ⁇ , ⁇ -diethylacetamide, or ⁇ , ⁇ -dimethylpropionamide may also be employed.
- Step B Ketalization of ER-811475 to ER-076349
- Ketalization of ER-81 1475 (e.g., in dichloromethane) with a conjugate acid of pyridine (e.g., pyridinium p-toluenesulfonate (PPTS)), followed by crystallization from acetonitrile and water, provides ER-076349.
- pyridine e.g., pyridinium p-toluenesulfonate (PPTS)
- ER-81 1475 to ER-076349 can be achieved through ketalization of ER-81 1475 (e.g., in ethanol) with a conjugate acid of imidazole (e.g., imidazole hydrochloride), followed by column chromatography.
- a conjugate acid of imidazole e.g., imidazole hydrochloride
- Replacing PPTS with imidazole hydrochloride results in a decrease of isomerization at C12 during post-processing (e.g., concentration of the reaction mixture).
- Changing of the solvent from dichloromethane to ethanol results in a more environmentally favorable process.
- Step C Activation of ER-076349 to a compound of formula (II)
- ER-076349 e.g., in dichloromethane
- tosyl chloride and a base e.g., pyridine
- a base e.g., pyridine
- ER-076349 e.g., in dichloromethane
- Ts 2 0 4-toluenesulfonic anhydride
- base e.g., a combination of 2,4,6-collidine and pyridine
- ER-076349 e.g., in acetonitrile
- a base e.g., a C1 -C6 trialkylamine, such as triethylamine and N,N- diisopropylethylamine
- a catalyst e.g., dibutyltin oxide
- dibutyltin oxide makes the process more robust (e.g., reduces reaction sensitivity to moisture) and improves process operational efficiency (e.g., by elimination of an azeotropic drying step).
- Replacing pyridine and/or collidine with A/,A/-diisopropylethylamine and the addition of dibutyltin oxide as a catalyst provide an improvement in selectivity for primary alcohol (e.g., the mono-tosylation:di-tosylation ratio improved from 96:4 to 99.8:0.2).
- the replacement of dichloromethane with acetonitrile as solvent results in a more environmentally favorable process, and the change in temperature from -10°C to 26 °C - 28°C increases operational efficiency and yield.
- Step D Amination of a compound of formula (II) to eribulin
- Method D 1 Staudinger route
- the amination of a compound of formula (II) can be accomplished through treatment with alcoholic ammonium hydroxide resulting in cyclization to an epoxide in situ that reacts further with ammonia to provide eribulin.
- alcoholic ammonium hydroxide resulting in cyclization to an epoxide in situ that reacts further with ammonia to provide eribulin.
- Replacement of the Staudinger route with the epoxide opening route results in the elimination of the use of hazardous reagents and an increase in operational efficiency.
- eribulin mesylate can be formed by methods known in the art (e.g., in situ during the final isolation and purification of the compound or separately by reacting the free base group with a suitable acid).
- eribulin is treated with a solution of methanesulfonic acid (i.e., MsOH) and ammonium hydroxide in water and acetonitrile. The mixture is concentrated. The residue is dissolved in dichloromethane-pentane, and the solution is added to anhydrous pentane. The resulting precipitate is filtered and dried under high vacuum to provide eribulin mesylate, as shown in Scheme 13.
- Any combination of the methods described above for the synthesis of the various intermediates can be utilized to convert a compound of formula (I) to eribulin (e.g., Methods A1 -B2-C1 -D1 , A1 -B2-C2- D1 , A1 -B2-C1 -D2, A2-B1 -C1 -D1 , A2-B2-C1 -D1 , A2-B1 -C2-D1 , A2-B1 -C1 -D2, A2-B2-C2-D1 , A2-B2-C1 - D2, A2-B1 -C2-D2, A2-B2-C2-D2, A2-B1 -C3-D1 , A1 -B2-C3-D1 , A2-B2-C3-D1 , A2-B2-C3-D1 , A2-B2-C3-D1 , A2-B2-C3-D1 , A2-B2-C3-D1 , A2-B2-
- Step A Conversion of ER- 118046 to ER-811475
- ER-81 1475 (1 F?,2S,3S,4S,5S,6F?S,1 1 S,14S,17S,19F?,21 R,23S,25R,26R,27S,31 F?,34S)-25-[(2S)-2,3- Dihydroxypropyl]- 2,5-dihydroxy-26-methoxy-19-methyl-13,20-bis(methylene)-24,35,36,37,38,39- hexaoxaheptacyclo[29.3.1 .13,6.14,34.1 1 1 ,14.1 17,21 .023,27]nonatriacontane-8,29-dione
- reaction intermediate C34/C35-diol After the residual level of the reaction intermediate C34/C35-diol reached 3% or below, acetonitrile (608 m l_) and water (203 ml_) were added. The mixture was stirred at 16 °C to 18 °C for 45 hours until the residual level of free pentaol came down to below 5%.
- the reaction mixture including ER-81 1475/ER-81 1474 (a mixture of two diastereomers 18:1 ) could be used for the next stage without further purification.
- Step B Ketalization of ER-811475 to ER-076349
- ER-81 1475 in a mixture with ER-81 1474 was dissolved in dichloromethane (DCM ; 7.7 kg) and treated with a pyridinium p-toluenesulfonate (PPTS; 0.607 kg, 2.42 mol, 5.5 eq) solution in dichloromethane (1 .7 kg) at 10-20 °C.
- PPTS pyridinium p-toluenesulfonate
- the resulting mixture was stirred at 1 0-20 °C.
- the major diastereomer reacted to provide diol ER-076349, and the minor diastereomer ER-81 1474 remained unreacted.
- additional PPTS 0.055 kg
- dichloromethane (0.15 kg) was added, and the reaction was continued at 10-20 °C. Upon completion, the reaction mixture was directly loaded onto a silica gel column that was pre-equilibrated with methyl f-butyl ether (MTBE) (200 L). The reactor was further rinsed with dichloromethane (3.1 kg), and the rinse was loaded onto the column. The column was eluted sequentially with: (1 ) methyl t-butyl ether (125 L), (2) 96% v/v methyl t-butyl ether in acetonitrile (125 L), (3) 50% v/v methyl t-butyl ether in acetonitrile (250 L), and (4) acetonitrile (225 L).
- MTBE methyl f-butyl ether
- Desired fractions were combined, concentrated in vacuo ⁇ 35 °C, and azeotroped in vacuo with acetonitrile (4.6 kg) ⁇ 35 °C.
- the residue was dissolved in acetonitrile (0.32 kg) and water (0.54 kg) and subjected to crystallization with ER-076349 seed crystals (0.27 g, 0.36 mmol) and additional water (2.70 kg).
- the resulting crystals were filtered, and the weight of the filtrate was monitored until the recovery ratio to the crystallization solvent was reached > 80%.
- the crystals were further washed with water (2.7 kg) and dissolved in dichloromethane (10.8 kg), and the solution was concentrated in vacuo at ⁇ 25 °C.
- the residue was diluted with acetonitrile (2.1 kg) and concentrated in vacuo at ⁇ 40 °C to give ER-076349 (55-75% yield from ER-1 18046).
- ER-81 1475 in a mixture with ER-81 1474, a solution of imidazole HCI (85.5 g) in water (68 ml_) was added. The solution was concentrated in vacuo at 28 °C or below. The residue was dissolved in EtOH (2.69 kg). The resulting mixture was stirred at 21 °C to 24 °C for 43 hours. The major diastereomer (ER- 81 1475) reacted to provide diol ER-076349, and the minor diastereomer (ER-81 1474) remains unreacted. The reaction was monitored for a disappearance of ER-81 1475 by HPLC.
- the column was eluted sequentially with methyl t-butyl ether (40.8 L) , 95% v/v methyl t-butyl ether in acetonitrile (24.9 L), 40% v/v methyl t-butyl ether in acetonitrile (83.6 L) , and acetonitrile (76.3 L) to remove the unreacted intermediates, the reaction impurities, and the carryover impurities from ER-804028. Desired fractions were combined and concentrated in vacuo at 32 °C or below to give ER-076349 (assay 62.02g, yield over two steps 84.0%).
- ER-076349 (0.259 kg, 0.354 mol, 1 eq) was dissolved in toluene (4.7 kg) and azeotroped in vacuo at ⁇ 25 °C. The residue was diluted with toluene (4.5 kg) to give a toluene solution for monitoring of water content. Water content was measured by Karl-Fischer (KF) titration method. If the KF value was > 125 ppm , the solution was azeotroped in vacuo at ⁇ 25 °C and diluted with toluene (4.5 kg) until the water content came down to ⁇ 125 ppm .
- the reaction mixture was quenched by the addition of water (1 .0 kg). The mixture was warmed up, and then isopropyl alcohol (IPA) (20.5 kg) and ammonium hydroxide (NH 4 OH ; 25.7 kg) were added consecutively at 1 0-30 °C. Upon complete consumption of the epoxide (target ⁇ 0.85%; add extra NH 4 OH if necessary), the reaction mixture was concentrated in vacuo at ⁇ 30 °C.
- IPA isopropyl alcohol
- NH 4 OH ammonium hydroxide
- dichloromethane (20.7 kg) and a sufficient amount of buffer solution of NaHC0 3 /Na 2 C0 3 /water (9/9/182 w/w/w; not more than 5.166 kg) were added and extracted.
- the organic layer was separated, and the aqueous layer was extracted with dichloromethane (8.6 kg).
- the organic layer was separated and combined with the first extract.
- the combined organic layers were concentrated in vacuo at ⁇ 30 °C.
- the concentrate was diluted with acetonitrile (4.0 kg) and then loaded onto silica gel column which was preequilibrated with acetonitrile (200 L).
- the column was eluted sequentially with : (1 ) acetonitrile (100 L), (2) 90.0/7.5/2.5 v/v/v acetonitrile/water/200 mM aqueous NH 4 OAc (152.4 L), (3) 85.8/1 1 .7/2.5 v/v/v acetonitrile/water/200 mM aqueous NH 4 OAc (152.4 L), (4) 83.5/14.0/2.5 v/v/v acetonitrile/water/200 mM aqueous NH 4 OAc (152.6 L) , and (5) 80.0/17.6/2.4 v/v/v acetonitrile/water/200 mM aqueous NH 4 OAc (>1 00.2 L).
- Desired fractions were combined and concentrated in vacuo at ⁇ 40 °C while maintaining the internal pH at 5.5-9.0 by adding NH 4 OH.
- dichloromethane (13.9 kg) and a sufficient amount of buffer solution of NaHC0 3 /Na 2 C0 3 /water (9/9/182 w/w/w; not more than 1 5.51 kg) were added and extracted.
- the organic layer was separated, and the aqueous layer was extracted with dichloromethane (8.7 kg).
- the organic layer was separated and combined with the first extract.
- the combined organic layers were concentrated in vacuo at ⁇ 30 °C.
- the diol ER-076349 (58.3 g) was dissolved in acetonitrile (935 m L). A suspension of dibutyltin oxide (0.99 g) and A/,A/-diisopropylethylamine (28.5 m L) in acetonitrile (1 1 7 m L) were added. A solution of TsCI (30.5 g) in acetonitrile (1 17 mL) was added to the reaction mixture at a rate to maintain the reaction temperature at 26 °C to 28 °C, and the mixture was stirred at 26 °C to 28 °C. The reaction was monitored by H PLC for consumption of ER-076349.
- dichloromethane (4.64 kg) and a sufficient amount of buffer solution of NaHC0 3 / Na 2 C0 3 / water (9/9/182 w/w/w) (530 mL) were added and extracted.
- the organic layer was separated, and the aqueous layer was extracted with dichloromethane (1 .94 kg).
- the organic layer was separated, and combined with the first extract.
- the combined organic layers were concentrated in vacuo at 25 °C or below.
- the concentrate was diluted with acetonitrile (1 .17 L), and then loaded onto silica gel column (5.51 1 kg) which was pre-equilibrated with acetonitrile (more than 55.1 L).
- the column was eluted sequentially with acetonitrile (29.6 L), 90.0/7.5/2.5 v/v/v acetonitrile/water/200 mM aqueous NH 4 OAc (46.2 L), 85.8/1 1 .7/2.5 v/v/v acetonitrile/water/200 mM aqueous NH 4 OAc (45.8 L), 83.5/14.0/2.5 v/v/v acetonitrile/water/200 mM aqueous NH 4 OAc (46.5 L), 80.0/17.6/2.4 v/v/v acetonitrile/water/200 mM aqueous NH 4 OAc (29.8 L) to remove the unreacted intermediates and the reaction impurities.
- Desired fractions were combined and concentrated in vacuo at 36 °C or below while maintaining the internal pH at 5.5 to 9.0 by adding ammonium hydroxide.
- dichloromethane (3.98 kg) and a sufficient amount of buffer solution of NaHC0 3 / Na 2 C0 3 / water (9/9/182 w/w/w) (2.02 kg) were added and extracted.
- the organic layer was separated, and the aqueous layer was extracted with dichloromethane (2.48 kg).
- the organic layer was separated and combined with the first extract.
- the combined organic layers were concentrated in vacuo at 24 °C or below.
- the residue was dissolved in 75% v/v anhydrous dichloromethane in n-pentane (1 .03 L) and filtered.
- the filtrate was concentrated in vacuo at 25 °C or below to give eribulin.
- the residue was diluted with acetonitrile (392 m L) and dichloromethane (69 m L) to give eribulin acetonitrile/dichloromethane solution (assay 49.1 1 g, corrected yield 85.3%).
- the solution was concentrated in vacuo at 29 °C or below and used for the next stage.
- ER-086526-00 (46.68 g) was dissolved in acetonitrile (591 mL) and water (31 m L) and treated with a solution of methanesulfonic acid (MsOH, 4.09 m L) and NH 4 OH (187 mL) in acetonitrile (624 m L). The mixture was concentrated in vacuo at 24 °C or below and azeotroped repeatedly with anhydrous acetonitrile (234 mL) in vacuo at 24 °C or below to remove water. The residue was dissolved in 75% v/v anhydrous dichloromethane in n-pentane (1 .10 L) and filtered.
- the filtrate was concentrated in vacuo at 24 °C or below.
- the residue was dissolved in 50% v/v anhydrous dichloromethane in n-pentane (1 .16 L), and the solution was transferred through a filter to anhydrous pentane (3.26 kg) in the separate reactor.
- the resulting precipitate was stirred for 29 hours.
- the precipitates were filtered, washed with n-pentane (2.92 kg), and dried under nitrogen flow in vacuo until the residual solvent levels reached the target numbers: n-pentane ⁇ 25000 ppm ; 2-methylbutane ⁇ 1000 ppm ; 2,2-dimethylbutane ⁇ 1000 ppm ; and cyclopentane ⁇ 1000 ppm.
- eribulin mesylate (gross 45.95 g, corrected yield 83.8%).
- the drug substance was filled in a polytetrafluoroethylene (PTFE) bottle.
- the PTFE bottle was packed in an aluminum laminate bag.
Abstract
Description
Claims
Priority Applications (15)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2931788A CA2931788C (en) | 2013-12-06 | 2014-12-05 | Methods useful in the synthesis of halichondrin b analogs |
BR112016012237-2A BR112016012237B1 (en) | 2013-12-06 | 2014-12-05 | USEFUL METHODS IN THE SYNTHESIS OF HALICONDRIN B ANALOGUES |
SG11201604545XA SG11201604545XA (en) | 2013-12-06 | 2014-12-05 | Methods useful in the synthesis of halichondrin b analogs |
MX2016006891A MX370611B (en) | 2013-12-06 | 2014-12-05 | Methods useful in the synthesis of halichondrin b analogs. |
ES14866945T ES2723355T3 (en) | 2013-12-06 | 2014-12-05 | Useful methods in the synthesis of halicondrine B analogues |
EP14866945.0A EP3077399B1 (en) | 2013-12-06 | 2014-12-05 | Methods useful in the synthesis of halichondrin b analogs |
MX2020004855A MX2020004855A (en) | 2013-12-06 | 2014-12-05 | Methods useful in the synthesis of halichondrin b analogs. |
CN201480066353.XA CN105916861B (en) | 2013-12-06 | 2014-12-05 | It can be used for the method for synthesis of halichondrin b analogs |
EP18209462.3A EP3505520A1 (en) | 2013-12-06 | 2014-12-05 | Methods useful in the synthesis of halichondrin b analogs |
RU2016124265A RU2676486C1 (en) | 2013-12-06 | 2014-12-05 | Methods for synthesis of halichondrin b analogs |
JP2016532033A JP6443812B2 (en) | 2013-12-06 | 2014-12-05 | Useful methods for the synthesis of halichondrin B analogs |
IL245661A IL245661A0 (en) | 2013-12-06 | 2016-05-16 | Methods useful in the synthesis of halichondrin b analogs |
IL265640A IL265640B (en) | 2013-12-06 | 2019-03-26 | Methods useful in the synthesis of halichondrin b analogs |
IL273736A IL273736B (en) | 2013-12-06 | 2020-04-01 | Methods useful in the synthesis of halichondrin b analogs |
IL281275A IL281275B (en) | 2013-12-06 | 2021-03-04 | Methods useful in the synthesis of halichondrin b analogs |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361912714P | 2013-12-06 | 2013-12-06 | |
US61/912,714 | 2013-12-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2015085193A1 true WO2015085193A1 (en) | 2015-06-11 |
Family
ID=53270478
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2014/068834 WO2015085193A1 (en) | 2013-12-06 | 2014-12-05 | Methods useful in the synthesis of halichondrin b analogs |
Country Status (13)
Country | Link |
---|---|
US (4) | US9303039B2 (en) |
EP (2) | EP3077399B1 (en) |
JP (3) | JP6443812B2 (en) |
CN (1) | CN105916861B (en) |
BR (1) | BR112016012237B1 (en) |
CA (1) | CA2931788C (en) |
ES (1) | ES2723355T3 (en) |
HU (1) | HUE043088T2 (en) |
IL (4) | IL245661A0 (en) |
MX (3) | MX2020004855A (en) |
RU (1) | RU2676486C1 (en) |
SG (2) | SG11201604545XA (en) |
WO (1) | WO2015085193A1 (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9938288B1 (en) | 2017-04-05 | 2018-04-10 | President And Fellows Of Harvard College | Macrocyclic compound and uses thereof |
USRE46965E1 (en) | 2004-06-03 | 2018-07-24 | Eisai R&D Management Co., Ltd. | Intermediates for the preparation of analogs of Halichondrin B |
WO2018187331A1 (en) | 2017-04-05 | 2018-10-11 | President And Fellows Of Harvard College | Macrocyclic compound and uses thereof |
JP2019504839A (en) * | 2016-02-12 | 2019-02-21 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | Intermediates and related synthetic methods in the synthesis of eribulin |
WO2019099646A1 (en) | 2017-11-15 | 2019-05-23 | President And Fellows Of Harvard College | Macrocyclic compounds and uses thereof |
US10322192B2 (en) | 2016-03-02 | 2019-06-18 | Eisai R&D Management Co., Ltd. | Eribulin-based antibody-drug conjugates and methods of use |
US10344038B2 (en) | 2015-04-30 | 2019-07-09 | President And Fellows Of Harvard College | Chromium-mediated coupling and application to the synthesis of halichondrins |
US10392400B2 (en) | 2016-11-11 | 2019-08-27 | President And Fellows Of Harvard College | Palladium-mediated ketolization |
US10556910B2 (en) | 2014-06-30 | 2020-02-11 | President And Fellows Of Harvard College | Synthesis of halichondrin analogs and uses thereof |
WO2020016847A3 (en) * | 2018-07-20 | 2020-03-12 | Dr. Reddy’S Laboratories Limited | Purification process for preparation of eribulin and intermediates thereof |
WO2020075027A1 (en) * | 2018-10-09 | 2020-04-16 | Dr. Reddy's Laboratories Limited | Process for preparation of eribulin and intermediates thereof |
WO2022101931A1 (en) * | 2020-11-10 | 2022-05-19 | Natco Pharma Limited | Process for the preparation of eribulin and its intermediates |
US11419856B2 (en) | 2017-11-20 | 2022-08-23 | Basilea Pharmaceutica International AG | Pharmaceutical combinations for use in the treatment of neoplastic diseases |
US11498892B2 (en) | 2017-07-06 | 2022-11-15 | President And Fellows Of Harvard College | Fe/Cu-mediated ketone synthesis |
US11548898B2 (en) | 2017-07-06 | 2023-01-10 | President And Fellows Of Harvard College | Synthesis of halichondrins |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009046308A1 (en) | 2007-10-03 | 2009-04-09 | Eisai R & D Management Co., Ltd. | Intermediates and methods for the synthesis of halichondrin b analogs |
US8598373B2 (en) | 2008-04-04 | 2013-12-03 | Eisai R&D Management Co., Ltd. | Halichondrin B analogs |
RU2579511C2 (en) | 2010-01-26 | 2016-04-10 | Эйсай Ар Энд Ди Менеджмент Ко., Лтд. | Furo[3,2-b]pyran derivatives, applicable in synthesis of analogues |
HUE049387T2 (en) | 2013-11-04 | 2020-09-28 | Eisai R&D Man Co Ltd | Macrocyclization reactions and intermediates useful in the synthesis of analogs of halichondrin b |
US9303039B2 (en) * | 2013-12-06 | 2016-04-05 | Eisai R&D Management Co., Ltd. | Methods useful in the synthesis of halichondrin B analogs |
CN107849057B (en) | 2015-05-07 | 2020-11-10 | 卫材R&D管理有限公司 | Macrocyclization reactions and intermediates and other fragments for the synthesis of halichondrin macrolides |
US11136335B2 (en) | 2016-06-30 | 2021-10-05 | Eisai R&D Management Co., Ltd. | Prins reaction and intermediates useful in the synthesis of halichondrin macrolides and analogs thereof |
WO2018217894A1 (en) * | 2017-05-24 | 2018-11-29 | Eisai R&D Management Co., Ltd. | Fluorine-labelled halichondrin derivatives and related methods of synthesis |
CN109694379B (en) * | 2017-10-24 | 2020-09-11 | 江苏恒瑞医药股份有限公司 | Intermediate for preparing eribulin and preparation method thereof |
IL275729B2 (en) | 2018-01-03 | 2023-09-01 | Eisai R&D Man Co Ltd | Prins reaction and compounds useful in the synthesis of halichondrin macrolides and analogs thereof |
WO2020110146A1 (en) * | 2018-11-28 | 2020-06-04 | Natco Pharma Limited | Process for the preparation of high pure eribulin and its mesylate salt |
US11447499B2 (en) | 2019-06-14 | 2022-09-20 | Rk Pharma Inc. | Process for the preparation of eribulin mesylate intermediate |
CN113354659B (en) * | 2021-06-08 | 2022-04-08 | 江苏慧聚药业股份有限公司 | Synthesis of eribulin mesylate |
CN114380840B (en) * | 2022-01-27 | 2024-01-26 | 江苏慧聚药业股份有限公司 | Synthesis of eribulin |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6194586B1 (en) * | 1996-09-06 | 2001-02-27 | Eli Lilly And Company | Selective sulphonation of the primary alcohol of a diol containing both primary and secondary alcohols |
US6214865B1 (en) | 1998-06-17 | 2001-04-10 | Eisai Co., Ltd. | Macrocyclic analogs and methods of their use and preparation |
US20070244187A1 (en) * | 2004-06-03 | 2007-10-18 | Eisai Co., Ltd | Intermediates for the Preparation of Halichondrin B |
Family Cites Families (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU652180A1 (en) | 1975-12-16 | 1979-03-15 | Краснодарский политехнический институт | Method of obtaining 1-(furyl-2')-2-(2-"formyl-furyl-5") ethylene and its methyl derivative as intermediate product for synthesis of furan-2,5-dicarboxylic acid |
US5338865A (en) | 1992-03-12 | 1994-08-16 | President And Fellows Of Harvard College | Synthesis of halichondrin B and norhalichondrin B |
US5436238A (en) | 1992-03-12 | 1995-07-25 | President And Fellows Of Harvard College | Halichondrins and related compounds |
GB9206244D0 (en) | 1992-03-23 | 1992-05-06 | Pharma Mar Sa | Cytotoxic compound from a marine sponge |
TW255880B (en) | 1992-09-09 | 1995-09-01 | Hoechst Ag | |
US6653341B1 (en) | 1998-06-17 | 2003-11-25 | Eisai Co., Ltd. | Methods and compositions for use in treating cancer |
US8097648B2 (en) | 1998-06-17 | 2012-01-17 | Eisai R&D Management Co., Ltd. | Methods and compositions for use in treating cancer |
CN1993342A (en) * | 2004-06-03 | 2007-07-04 | 卫材株式会社 | Intermediates for the preparation of halichondrin b |
US20060045846A1 (en) | 2004-08-30 | 2006-03-02 | Horstmann Thomas E | Reagents and methods for labeling terminal olefins |
JP2008522623A (en) | 2004-12-09 | 2008-07-03 | エーザイ株式会社 | Screening for tubulin isotypes in cancer treatment using halichondrin B analogs |
WO2008010776A1 (en) | 2006-07-21 | 2008-01-24 | Agency For Science, Technology And Research | Aigialomycin d and derivatives thereof and their use in treating cancer or malaria or a microbial infection |
JPWO2009014105A1 (en) | 2007-07-20 | 2010-10-07 | 株式会社山田養蜂場本社 | NOVEL CARBOXYLIC ACID AND ANTI-DEPRESSION COMPOSITION CONTAINING THE SAME |
WO2009046308A1 (en) * | 2007-10-03 | 2009-04-09 | Eisai R & D Management Co., Ltd. | Intermediates and methods for the synthesis of halichondrin b analogs |
CN101910180A (en) | 2007-11-16 | 2010-12-08 | 卫材R&D管理有限公司 | Novel intermediate for halichondrin B analog synthesis and novel desulfonylation reaction used for the intermediate |
US8598373B2 (en) | 2008-04-04 | 2013-12-03 | Eisai R&D Management Co., Ltd. | Halichondrin B analogs |
JP5371091B2 (en) | 2009-01-23 | 2013-12-18 | 三菱レイヨン株式会社 | Method for producing monosulfonic acid ester |
RU2579511C2 (en) | 2010-01-26 | 2016-04-10 | Эйсай Ар Энд Ди Менеджмент Ко., Лтд. | Furo[3,2-b]pyran derivatives, applicable in synthesis of analogues |
EP2354128A1 (en) | 2010-02-10 | 2011-08-10 | Sandoz Ag | Method for the preparation of rivaroxaban |
WO2012147900A1 (en) | 2011-04-28 | 2012-11-01 | Eisai R&D Management Co., Ltd. | Microreactor process for halichondrin b analog synthesis |
WO2013078559A1 (en) | 2011-11-30 | 2013-06-06 | Alphora Research Inc. | Process for preparation of (3r)-2,4-di-leaving group-3-methylbut-1-ene |
JP6531911B2 (en) | 2012-03-30 | 2019-06-19 | サンド アーゲー | Synthetic process for preparing macrocyclic C1-keto analogues of halichondrin B and useful intermediates therefor |
EP3016957A4 (en) | 2013-07-03 | 2016-11-30 | Alphora Res Inc | Synthetic process for preparation of macrocyclic c1-keto analogs of halichondrin b and intermediates useful therein including intermediates containing -so2-(p-tolyl) groups |
HUE049387T2 (en) | 2013-11-04 | 2020-09-28 | Eisai R&D Man Co Ltd | Macrocyclization reactions and intermediates useful in the synthesis of analogs of halichondrin b |
US9303039B2 (en) * | 2013-12-06 | 2016-04-05 | Eisai R&D Management Co., Ltd. | Methods useful in the synthesis of halichondrin B analogs |
CN107849057B (en) | 2015-05-07 | 2020-11-10 | 卫材R&D管理有限公司 | Macrocyclization reactions and intermediates and other fragments for the synthesis of halichondrin macrolides |
EP3413887B1 (en) | 2016-02-12 | 2021-04-07 | Eisai R&D Management Co., Ltd. | Intermediates in the synthesis of eribulin and related methods of synthesis |
US11136335B2 (en) | 2016-06-30 | 2021-10-05 | Eisai R&D Management Co., Ltd. | Prins reaction and intermediates useful in the synthesis of halichondrin macrolides and analogs thereof |
-
2014
- 2014-12-05 US US14/562,086 patent/US9303039B2/en active Active
- 2014-12-05 CN CN201480066353.XA patent/CN105916861B/en active Active
- 2014-12-05 MX MX2020004855A patent/MX2020004855A/en unknown
- 2014-12-05 HU HUE14866945A patent/HUE043088T2/en unknown
- 2014-12-05 RU RU2016124265A patent/RU2676486C1/en active
- 2014-12-05 ES ES14866945T patent/ES2723355T3/en active Active
- 2014-12-05 MX MX2016006891A patent/MX370611B/en active IP Right Grant
- 2014-12-05 SG SG11201604545XA patent/SG11201604545XA/en unknown
- 2014-12-05 CA CA2931788A patent/CA2931788C/en active Active
- 2014-12-05 BR BR112016012237-2A patent/BR112016012237B1/en active IP Right Grant
- 2014-12-05 WO PCT/US2014/068834 patent/WO2015085193A1/en active Application Filing
- 2014-12-05 EP EP14866945.0A patent/EP3077399B1/en active Active
- 2014-12-05 SG SG10201805024YA patent/SG10201805024YA/en unknown
- 2014-12-05 EP EP18209462.3A patent/EP3505520A1/en active Pending
- 2014-12-05 JP JP2016532033A patent/JP6443812B2/en active Active
-
2016
- 2016-03-03 US US15/059,965 patent/US9695188B2/en active Active
- 2016-05-16 IL IL245661A patent/IL245661A0/en active IP Right Grant
- 2016-05-26 MX MX2019011926A patent/MX2019011926A/en active IP Right Grant
-
2017
- 2017-06-30 US US15/638,493 patent/US10030032B2/en active Active
-
2018
- 2018-07-10 US US16/031,629 patent/US10450324B2/en active Active
- 2018-10-16 JP JP2018194871A patent/JP6634135B2/en active Active
-
2019
- 2019-03-26 IL IL265640A patent/IL265640B/en active IP Right Grant
- 2019-12-13 JP JP2019225297A patent/JP6905041B2/en active Active
-
2020
- 2020-04-01 IL IL273736A patent/IL273736B/en active IP Right Grant
-
2021
- 2021-03-04 IL IL281275A patent/IL281275B/en unknown
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6194586B1 (en) * | 1996-09-06 | 2001-02-27 | Eli Lilly And Company | Selective sulphonation of the primary alcohol of a diol containing both primary and secondary alcohols |
US6214865B1 (en) | 1998-06-17 | 2001-04-10 | Eisai Co., Ltd. | Macrocyclic analogs and methods of their use and preparation |
US6365759B1 (en) | 1998-06-17 | 2002-04-02 | Eisai Co., Ltd. | Intermediate compounds for preparing macrocylcic analogs |
US6469182B1 (en) | 1998-06-17 | 2002-10-22 | Eisai Co., Ltd. | Intermediates in the preparation of macrocyclic analogs |
US8148554B2 (en) | 1998-06-17 | 2012-04-03 | Eisai R&D Management Co., Ltd. | Methods and compositions for use in treating cancer |
US20070244187A1 (en) * | 2004-06-03 | 2007-10-18 | Eisai Co., Ltd | Intermediates for the Preparation of Halichondrin B |
US7982060B2 (en) | 2004-06-03 | 2011-07-19 | Eisai R&D Management Co., Ltd. | Intermediates for the preparation of analogs of Halichondrin B |
Non-Patent Citations (3)
Title |
---|
CUNNINGHAM, LW ET AL.: "The Influence of pH on the Kinetic Constants of alpha-chymotrypsin-catalyzed Esterolysis';", J. BIOL. CHEM., 1956, pages 287 - 300, XP055346976 * |
GREENE, TW ET AL.: "tertbutyldimethylsilyl ether cleavage;", PROTECTIVE GROUPS IN ORGANIC SYNTHESIS;, 1999, pages 133 - 139, XP055346974 * |
See also references of EP3077399A4 |
Cited By (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
USRE46965E1 (en) | 2004-06-03 | 2018-07-24 | Eisai R&D Management Co., Ltd. | Intermediates for the preparation of analogs of Halichondrin B |
US10556910B2 (en) | 2014-06-30 | 2020-02-11 | President And Fellows Of Harvard College | Synthesis of halichondrin analogs and uses thereof |
US11155562B2 (en) | 2014-06-30 | 2021-10-26 | President And Fellows Of Harvard College | Synthesis of halichondrin analogs and uses thereof |
US11220513B2 (en) | 2015-04-30 | 2022-01-11 | President And Fellows Of Harvard College | Chromium-mediated coupling and application to the synthesis of halichondrins |
US10344038B2 (en) | 2015-04-30 | 2019-07-09 | President And Fellows Of Harvard College | Chromium-mediated coupling and application to the synthesis of halichondrins |
US10633392B2 (en) | 2015-04-30 | 2020-04-28 | President And Fellows Of Harvard College | Chromium-mediated coupling and application to the synthesis of halichondrins |
JP2019504839A (en) * | 2016-02-12 | 2019-02-21 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | Intermediates and related synthetic methods in the synthesis of eribulin |
US10322192B2 (en) | 2016-03-02 | 2019-06-18 | Eisai R&D Management Co., Ltd. | Eribulin-based antibody-drug conjugates and methods of use |
US10548986B2 (en) | 2016-03-02 | 2020-02-04 | Eisai R&D Management Co., Ltd. | Eribulin-based antibody-drug conjugates and methods of use |
US10844073B2 (en) | 2016-11-11 | 2020-11-24 | President And Fellows Of Harvard College | Palladium-mediated ketolization |
US10392400B2 (en) | 2016-11-11 | 2019-08-27 | President And Fellows Of Harvard College | Palladium-mediated ketolization |
US10954249B2 (en) | 2017-04-05 | 2021-03-23 | President And Fellows Of Harvard College | Macrocyclic compound and uses thereof |
JP7168580B2 (en) | 2017-04-05 | 2022-11-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Macrocycles and uses thereof |
JP2020513021A (en) * | 2017-04-05 | 2020-04-30 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Macrocycles and uses thereof |
US9938288B1 (en) | 2017-04-05 | 2018-04-10 | President And Fellows Of Harvard College | Macrocyclic compound and uses thereof |
JP7400025B2 (en) | 2017-04-05 | 2023-12-18 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Macrocycles and their uses |
WO2018187331A1 (en) | 2017-04-05 | 2018-10-11 | President And Fellows Of Harvard College | Macrocyclic compound and uses thereof |
US11725015B2 (en) | 2017-04-05 | 2023-08-15 | President And Fellows Of Harvard College | Macrocyclic compound and uses thereof |
EP4119563A2 (en) | 2017-04-05 | 2023-01-18 | President And Fellows Of Harvard College | Macrocyclic compound and uses thereof |
US11548898B2 (en) | 2017-07-06 | 2023-01-10 | President And Fellows Of Harvard College | Synthesis of halichondrins |
US11498892B2 (en) | 2017-07-06 | 2022-11-15 | President And Fellows Of Harvard College | Fe/Cu-mediated ketone synthesis |
US11814398B2 (en) | 2017-11-15 | 2023-11-14 | President And Fellows Of Harvard College | Macrocyclic compounds and uses thereof |
US11407762B2 (en) | 2017-11-15 | 2022-08-09 | President And Fellows Of Harvard College | Macrocyclic compounds and uses thereof |
WO2019099646A1 (en) | 2017-11-15 | 2019-05-23 | President And Fellows Of Harvard College | Macrocyclic compounds and uses thereof |
US11419856B2 (en) | 2017-11-20 | 2022-08-23 | Basilea Pharmaceutica International AG | Pharmaceutical combinations for use in the treatment of neoplastic diseases |
CN112437775A (en) * | 2018-07-20 | 2021-03-02 | 雷迪博士实验室有限公司 | Purification method for preparing eribulin and intermediates thereof |
WO2020016847A3 (en) * | 2018-07-20 | 2020-03-12 | Dr. Reddy’S Laboratories Limited | Purification process for preparation of eribulin and intermediates thereof |
EP3864011A4 (en) * | 2018-10-09 | 2022-06-29 | Dr. Reddy's Laboratories Ltd. | Process for preparation of eribulin and intermediates thereof |
WO2020075027A1 (en) * | 2018-10-09 | 2020-04-16 | Dr. Reddy's Laboratories Limited | Process for preparation of eribulin and intermediates thereof |
WO2022101931A1 (en) * | 2020-11-10 | 2022-05-19 | Natco Pharma Limited | Process for the preparation of eribulin and its intermediates |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US10450324B2 (en) | Methods useful in the synthesis of halichondrin B analogs | |
KR102634720B1 (en) | Methods for partitioning benzodiazepine-2-one and benzoazepine-2-one derivatives | |
RU2603261C2 (en) | Regioselective acylation of rapamycin at c-42 position | |
EP2640730B1 (en) | Processes for preparation of everolimus and intermediates thereof | |
EP2774926B1 (en) | Morphinan derivative | |
EP4086260A1 (en) | Solid state forms of remimazolam salts | |
MX2012013334A (en) | Process for the preparation of chiral triazolones. | |
WO2017021975A1 (en) | Process for the preparation of crystalline forms of rifaximin | |
EP4206205A1 (en) | Preparation method for pyrrolopyrimidine compound | |
JP6528251B2 (en) | Crystalline derivatives of (S) -1-((2R, 3R, 4S, 5S) -5-allyl-3-methoxy-4- (tosylmethyl) tetrahydrofuran-2-yl) -3-aminopropan-2-ol | |
WO2010089643A1 (en) | An improved process for the preparation of paliperidone | |
FR3022907A1 (en) | OSW-1 DERIVATIVES AND THEIR USE AS A MEDICINAL PRODUCT |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 14866945 Country of ref document: EP Kind code of ref document: A1 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 245661 Country of ref document: IL |
|
ENP | Entry into the national phase |
Ref document number: 2016532033 Country of ref document: JP Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 2931788 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: MX/A/2016/006891 Country of ref document: MX |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
REEP | Request for entry into the european phase |
Ref document number: 2014866945 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2014866945 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112016012237 Country of ref document: BR |
|
ENP | Entry into the national phase |
Ref document number: 2016124265 Country of ref document: RU Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: 112016012237 Country of ref document: BR Kind code of ref document: A2 Effective date: 20160530 |