WO2015076666A2 - Combinaison de ghréline et d'analogues de la kisspeptine (agonistes de gpr54) pour la croissance d'animaux aquatiques - Google Patents

Combinaison de ghréline et d'analogues de la kisspeptine (agonistes de gpr54) pour la croissance d'animaux aquatiques Download PDF

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Publication number
WO2015076666A2
WO2015076666A2 PCT/MY2014/000266 MY2014000266W WO2015076666A2 WO 2015076666 A2 WO2015076666 A2 WO 2015076666A2 MY 2014000266 W MY2014000266 W MY 2014000266W WO 2015076666 A2 WO2015076666 A2 WO 2015076666A2
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WIPO (PCT)
Prior art keywords
aquatic animals
peptides
growth
combination
stimulating growth
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PCT/MY2014/000266
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English (en)
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WO2015076666A3 (fr
Inventor
Ishwar PARHAR
Takashi KITAHASHI
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Monash University Malaysia Sdn. Bhd.
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Publication date
Application filed by Monash University Malaysia Sdn. Bhd. filed Critical Monash University Malaysia Sdn. Bhd.
Publication of WO2015076666A2 publication Critical patent/WO2015076666A2/fr
Publication of WO2015076666A3 publication Critical patent/WO2015076666A3/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH

Definitions

  • the present invention relates generally to the use of the combination of ghrelin and kisspeptin analogs (GPR54 agonists) for stimulating growth of aquatic animals such as fish and the method thereof.
  • GPR54 agonists ghrelin and kisspeptin analogs
  • GH Growth hormone
  • An orexigenic gut peptide ghrelin is a physiological mediator of feeding, and has a function in growth regulation by stimulating feeding and release of growth hormone (GH) from the pituitary.
  • GPR54 growth hormone
  • GPR54 have emerged as key players in the control of reproductive-axis function, in which they operate as primary regulators of hypothalamic gonadotropin-releasing hormone (GnRH) release. Stimulation of this system during early developmental stages might inhibit the reproductive system and lead to the stimulation of growth.
  • EP 1 178813B1 relates to a study in which aquatic animals are treated using growth hormones specifically bovine somatotropin (bsT).
  • Another study EP 191 1764 Bl relates to the use of polypeptides with amino acids sequences SEQ ID No. 7 and 8 to stimulate the growth of fish.
  • the genes that code said polypeptides were cloned in a Pichia pastoris expression vector which can be used to obtain extracellular protein.
  • EP 1686179 Al relates to the use of the combination of fertility hormones more particularly lutenizing hormone, follicle stimulating hormone and chorionic gonadotropin for improving the development and/or maturation of eggs in fish.
  • the combination of ghrelin and kisspeptin analogs provides a synergic effect to stimulate growth compared to individual treatments.
  • the present invention relates to the use of a combination of ghrelin and kisspeptin analogs (GPR54 agonists) for inhibiting sexual maturation and stimulating fish growth and the method thereof.
  • the method of this invention may be used for all other aquatic animals.
  • the treatment may be applied with other invertebrates such as lobsters, crabs, prawns, and shrimp and other aquatic vertebrates including amphibians.
  • the aquatic animals are selected from fish such as tilapia or like species.
  • the aquatic animals are preferably treated at any time between 0.5 to 3 month post- hatching.
  • the aquatic animals are treated in the culture water containing peptides, wherein the concentration preferably between 0.1 to 0.3 mg/L of ghrelin and 0.05 to 0.2 mg/L of kisspeptin analogs.
  • the treatment may also be administered to the aquatic animals orally as a component of a feed material.
  • the aquatic animals are subjected to a session of treatment which requires the aquatic animals to be immersed for a period that allows growth in the aquatic animals.
  • the aquatic animals are immersed in the solution for a period of between 0.5 to 3 hours.
  • the aquatic animals are treated preferably in interval about 1 to 4 times a week for the duration of 1 to 2 months.
  • the mean weight of the aquatic animals is measured each month to observe the changes of the growth of the aquatic animals.
  • FIG. 1 shows a schematic phylogenetic tree showing animal species that possess kisspeptin gene.
  • FIG. 2 shows a relationship between measurement dates against body weight of treated tilapia frys compared to the control group.
  • the present invention relates to the use of the combination of ghrelin and kisspeptin analogs (GPR54 agonists) for inhibiting sexual maturation and stimulating fish growth and the method thereof.
  • GPR54 agonists ghrelin and kisspeptin analogs
  • An orexigenic gut peptide ghrelin is a physiological mediator of feeding, and has a function in growth regulation by stimulating feeding and the release of growth hormone (GH) from the pituitary.
  • GH growth hormone
  • Kisspeptin and their intrinsic receptor, GPR54 have emerged as key players in the control of reproductive-axis function, in which they operate as primary regulators of hypothalamic gonadotropin-releasing hormone (GnRH) release. Stimulation of this system during early developmental stages inhibits the reproductive system and lead to the stimulation of growth.
  • Simultaneous treatment with ghrelin and kisspeptin analogs stimulates fish growth by the stimulation of GH release and the inhibition of reproductive system.
  • the method of this invention may be applied to all other aquatic animals equally as well.
  • the treatment may be applied with other invertebrates such as lobsters, crabs, prawns, and shrimp and other aquatic vertebrates including amphibians.
  • the aquatic animals are selected from fish such as tilapia or like species.
  • the treatment mechanism may be applied in these species.
  • a phylogenetic tree most fishes including salmon, fugu, sole, and grouper possess the kisspeptin gene, suggesting that the treatment may be applied in these fishes as well.
  • the aquatic animals are preferably treated when they are hatchlings.
  • the aquatic animals are treated at any time between 0.5 to 3 month post-hatching.
  • the size of the hatchlings is much smaller and the skins are subtle for absorption.
  • the peptides in culture water tend to penetrate into the aquatic animals body through the skin, gills, or the digestive system easily. Feeding these peptides to older fish may not be beneficial because the cost will be high. Also, the digestive systems might breakdown the peptides.
  • the aquatic animals are treated by immersion in the culture water containing a combination of ghrelin and kisspeptin analogs.
  • the aquatic animals are kept in shallow water to provide a synergic effect of the peptide treatment.
  • the treatment may be administered to the fish orally as a component of a feed material.
  • the aquatic animals are treated in the culture water containing peptides, wherein the concentration preferably between 0.1 to 0.3 mg/L of ghrelin and 0.05 to 0.2 mg/L of kisspeptin analogs.
  • the aquatic animals are subjected to a session of treatment which requires the aquatic animals to be immersed for a period that allows growth in the aquatic animals.
  • the aquatic animals are immersed in the solution for a period of between 0.5 to 3 hours.
  • the aquatic animals are treated preferably in interval for about 1 to 4 times a week, for duration of 1 to 2 months.
  • the mean weight of frys are measured each month to observe the changes of the growth of the aquatic animals.
  • the experimental groups were:
  • Tl Group treated with the water containing ghrelin
  • T2 Group treated with the water containing ghrelin and kisspeptin
  • T3 Group treated with the water containing ghrelin and kisspeptin
  • the treatment was carried using 1 month old post-hatching tilapia frys.
  • the frys were kept in a 1 ton fiberglass tank (2 m X 1 m X 50 cm) with river water supply under natural photo regimen.
  • water in the fiberglass tank was discharged to make it 100 L (5 cm depth).
  • Stock solutions of the peptides (10 mg/mL) were added to the tank to make them the final concentration of 0.1 mg/L (kisspeptin analogs) and 0.2 mg/L (ghrelin), respectively.
  • water supply was resumed to make the normal water level.
  • the treatment was repeated 3 times a week for duration of 1 month.
  • the mean weight of frys were measured from 1 st to 5 th month and the results are tabulated in Table 1.
  • the experiment was also carried out with groups C, Tl and T2.
  • Table 1 Mean weight measured in the different groups of tilapia frys With respect to the trends of the data in Table 1 and Figure 2, it can be observed that the fish treated with ghrelin and kisspeptin analogs were larger that the untreated fish. In fact, treatment with the combination of ghrelin and kisspeptin analogs during the juvenile stage significantly enhanced the growth of tilapia compared to the control and ghrelin treated groups. This synergistic effect was probably due to the stimulated food intake and GH release by ghrelin and the inhibition of sexual maturation by kisspeptin analogs.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Birds (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Feed For Specific Animals (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

La présente invention concerne un procédé pour stimuler la croissance d'animaux aquatiques ou l'utilisation d'une combinaison de peptides pour stimuler la croissance d'animaux aquatiques, lesdits peptides étant choisis entre la ghréline et des analogues de la kisspeptine.
PCT/MY2014/000266 2013-11-25 2014-11-25 Combinaison de ghréline et d'analogues de la kisspeptine (agonistes de gpr54) pour la croissance d'animaux aquatiques WO2015076666A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2013702246A MY167221A (en) 2013-11-25 2013-11-25 Combination of ghrelin and kisspeptin analogs (gpr54 agonists) for growth of aquatic animals
MYPI2013702246 2013-11-25

Publications (2)

Publication Number Publication Date
WO2015076666A2 true WO2015076666A2 (fr) 2015-05-28
WO2015076666A3 WO2015076666A3 (fr) 2015-08-13

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PCT/MY2014/000266 WO2015076666A2 (fr) 2013-11-25 2014-11-25 Combinaison de ghréline et d'analogues de la kisspeptine (agonistes de gpr54) pour la croissance d'animaux aquatiques

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MY (1) MY167221A (fr)
WO (1) WO2015076666A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105104269A (zh) * 2015-09-02 2015-12-02 通威股份有限公司 一种罗非鱼的池塘精养养殖方法
CN107494364A (zh) * 2017-09-20 2017-12-22 苏州市相城区阳澄湖镇剑成水产生态养殖专业合作社 长江蟹的养殖方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102273566B (zh) * 2011-07-12 2013-01-02 中山大学 一种石斑鱼低鱼粉饲料

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105104269A (zh) * 2015-09-02 2015-12-02 通威股份有限公司 一种罗非鱼的池塘精养养殖方法
CN107494364A (zh) * 2017-09-20 2017-12-22 苏州市相城区阳澄湖镇剑成水产生态养殖专业合作社 长江蟹的养殖方法

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MY167221A (en) 2018-08-14
WO2015076666A3 (fr) 2015-08-13

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