WO2015045162A1 - Plant growth promoter and plant growth promotion method - Google Patents

Abstract

Provided is a plant growth promoter having an obvious effective component, capable of being applied to plants for which safety is required such as edible plants, and capable of applying a stable effect.　The plant growth promoter includes at least one type of sanguinarine indicated by formula (I) or a salt thereof, as an effective component.

Description

Plant growth promoting agent and plant growth promoting method

The present invention relates to a plant growth promoter and a plant growth promotion method capable of promoting plant growth and improving yield per unit area.

In the fields of agriculture and horticulture, promoting plant growth and improving yield per unit area is an important theme, and therefore various plant growth techniques are being studied. Of these technologies, special equipment is not required, and it is easy to introduce into normal work processes. Therefore, technology that applies plant growth promoters that have the effect of promoting plant growth to plants and soil has attracted attention. Yes. As plant growth promoters, it has been reported that biological materials such as culture solutions of various microorganisms (Patent Documents 1 and 2) and animal and plant extracts are used.

On the other hand, it is known that plant alkaloids having a benzophenanthridine skeleton such as sanguinarine are included in the species of poppy family, Macleaya cordata, and Eschscholzia californica. In recent years, research focused on this plant alkaloid has been conducted, and sanguinarine and the like have antibacterial action (Patent Documents 3 to 5), antitumor action (Patent Document 6), and pest control action (Patent Documents 7 and 8). It has been reported that there is such an effect.

JP 2009-249301 A JP 2009-091256 A Special table 2008-524130 gazette Special table 2011-521982 gazette JP-A-5-124944 Special table 2011-512370 gazette JP 2008-37775 A JP 2009-46411 A

However, the biological materials such as the microorganism culture solution described in Patent Documents 1 and 2 have a problem in safety when applied to edible plants because the active ingredients are unknown. In addition, since the biological material is obtained from the biological material, the effect sometimes varies.

Moreover, although plant alkaloids having a benzophenanthridine skeleton such as sanguinarine have a specific action as described in Patent Documents 3 to 8, it is reported that sanguinarine or the like is used to promote plant growth. It has not been.

Accordingly, an object of the present invention is to provide a new plant growth promoter that can be applied to plants that have a clear active ingredient and that require safety such as edible plants and that can provide a stable effect. It is in.

Another object of the present invention is to find a new bioactive action and use of a plant alkaloid having a benzophenanthridine skeleton such as sanguinarine.

Another object of the present invention is to provide a plant growth promoting method by using the above-mentioned plant growth promoter.

In order to solve the above problems, the plant growth promoter of the present invention is represented by the following formula (I):

Is contained as an active ingredient.

The plant growth promoter of the present invention contains as an active ingredient at least one of sanguinarine, which is a kind of plant alkaloid represented by the above formula, or a salt thereof. By applying the plant growth promoter of the present invention to plants, plant growth can be promoted and the yield can be improved. Furthermore, the appearance quality, taste, and other components preferable for health increase, so that the quality can be improved. Since sanguinarine or a salt thereof, which is an active ingredient, has a certain effect when applied at a low concentration of 1 mg / m ² or less and in a small amount, it can impart a stable plant growth promoting effect to plants. In addition, this sanguinarine has high safety, as reported in Non-patent Document 1 as a result of administration tests to rats, it was reported that there were no obstacles that should be noted in terms of physiological disorders. Yes. Therefore, it can be applied to plants that have high safety and require safety such as edible plants. The sanguinarine or a salt thereof in the present invention includes a sanguinarine derivative or a salt thereof.

It is also preferable that the active ingredient of the plant growth promoter of the present invention is a plant-derived product containing at least one of sanguinarine or a salt thereof. Thereby, the growth promotion of the plant made into object can be aimed at by the sanguinarine or its salt contained in a plant origin. Moreover, since the active ingredient of the growth promoter of the present invention can be obtained from a natural product, the plant growth promoter of the present invention can be obtained without relying on chemical means such as chemical synthesis.

It is also preferred that the plant-derived material is a bamboo rush-derived material. Thereby, a plant material suitable as a plant-derived material is selected. The growth of the target plant can be promoted by sanguinarine or a salt thereof contained in the bamboo rush.

Furthermore, it is also preferred that the bamboo rush-derived material is a bamboo rush seed-derived material. Thereby, a suitable organ containing many active ingredients is selected from the plant organs of the bamboo rush.

The plant-derived material is subjected to at least one extraction process selected from the group consisting of a solvent extraction process, a subcritical water extraction process, and a heat extraction process on a plant containing at least one of sanguinarine or a salt thereof. It is also preferable that the extract is obtained by Thereby, the active ingredient of the plant growth promoter of the present invention can be efficiently obtained by simple processes such as a solvent extraction process, a subcritical water extraction process, and a heat extraction process.

Furthermore, the plant growth promotion method of the present invention includes a step of applying the above-described plant growth promoter to a plant, soil, or a culture medium for hydroponics. By applying a plant growth promoter to a plant body, soil, or a culture solution of hydroponics like water or compost, a growth promoting effect can be easily imparted to the target plant.

In the plant growth promotion method of the present invention, the plant, soil, or hydroponics culture solution to which the plant growth promoter is applied may be a plant body during seedling, seedling culture soil, or a culture solution during seedling raising. preferable. By applying a plant growth promoter to a plant at the seedling stage before planting in a field, a growth promoting effect can be sufficiently imparted to the target plant. Thereby, compared with the application to a plant body after fixed planting, while being able to reduce the application amount of a plant growth promoter, the production cost of a plant can be reduced without taking the effort of application.

ADVANTAGE OF THE INVENTION According to this invention, the plant growth promoter which has the following outstanding effects can be provided.
(1) Since the active ingredient is at least one of sanguinarine or a salt thereof, the safety is high and the effect is exhibited at a low concentration.
(2) It has a remarkable plant growth promoting effect, and the effect can be obtained stably.
(3) The quality of the harvest can be improved by improving the quality of the appearance and taste of the harvest.
(4) Since the active ingredient can be easily obtained as a plant-derived material from a plant containing at least one of sanguinarine or a salt thereof, the plant of the present invention can be produced at low cost without relying on chemical means such as chemical synthesis. A growth promoter can be obtained.

The plant growth promoter of the present invention has the following formula (I) as an active ingredient:

Is contained as an active ingredient. Sanguinarine is a plant alkaloid having a benzophenanthridine skeleton that is mainly contained in poppy plants in nature. Furthermore, sanguinarine or a salt thereof in the present invention includes a sanguinarine derivative or a salt thereof having substantially the same function or effect as sanguinarine. In the present specification, what is hereinafter referred to as “sanguinarine” includes sanguinarine or a salt thereof as well as sanguinarine or a salt thereof.

The sanguinarine contained in the plant growth promoter of the present invention may be a natural product such as a natural extract, a chemically synthesized product, or a microbially derived product obtained by a microbial fermentation method, but without depending on chemical means such as chemical synthesis. From the viewpoint of obtaining the growth promoter of the present invention, a naturally derived product is preferable, and a plant derived product is more preferable. The sanguinarine salt is not particularly limited, but is an inorganic acid salt such as carbonate, hydrochloride, nitrate, sulfate or phosphate, and an organic acid salt such as acetate, propionate, butyrate or fatty acid salt. Is mentioned.

Sanguinarine is contained in plant bodies such as Takenigusa (Macleaya cordata), Hanabushisou (Eschscholzia californica), celandine (Chelidonium majus), and Bradroot (Sangurinaria canadensis). Therefore, it can be obtained by extracting sanguinarine, which is an active ingredient of the growth promoter of the present invention, from a plant containing such sanguinarine or a salt thereof.

Of these, bamboo rush is a perennial of the poppy family and is often found in sunny grasslands, open spaces, etc., and the bamboo rush that lives in such places is said to have a high content of sanguinarine. Accordingly, when sanguinarine is derived from a natural product, that is, from a plant, it is preferably obtained from a bamboo rush. Moreover, in bamboo rush, sanguinarine is contained in any plant organs of seeds, leaves, stems, fruits and roots, but the content in seeds is overwhelmingly higher than in other plant organs. Therefore, from the viewpoint of high efficiency, it is preferable to obtain sanguinarine from the seeds of bamboo rush. In addition, since the seeds of bamboo rush are contained in the strawberry fruit, it is also preferable to first harvest the strawberry fruit and use the seed taken out from the strawberry fruit or use the whole strawberry fruit. In addition, bamboo rushes can be harvested twice a year (early summer and late autumn) to produce strawberry fruits when they are cultivated in the natural environment. it can.

In the case of obtaining sanguinarine from a plant such as bamboo rush, the plant-derived product may contain other components contained in the plant. As an example, in addition to sanguinarine, the bamboo rush-derived material may contain alkaloids contained in bamboo rush, such as chelerythrine, cherylrubine, and protopine. Among these, chelerythrine is an alkaloid having a benzophenanthridine skeleton, like sanguinarine, which is an active ingredient of the growth promoter of the present invention, and has a structure that is almost in common with sanguinarine. Furthermore, both sanguinarine and chelerythrine are known to have protein kinase C (PKC) antagonist activity, antibacterial activity, and antifungal properties, and their functions and actions are common. Therefore, chelerythrine can be used as an active ingredient of the growth promoter of the present invention as a sanguinarine derivative.

Hereinafter, a method for obtaining sanguinarine which is an active ingredient of the growth promoter of the present invention from a plant containing sanguinarine will be described. Plant-derived products containing sanguinarine include the plant itself and plant extracts. For example, examples of the plant itself include those obtained by drying and crushing a plant containing sanguinarine, and those obtained by pulverizing the dried crushed material into a powder, granule, slurry, or paste.

On the other hand, the plant extract according to the present invention is obtained by performing an extraction treatment by immersing or contacting a plant material containing sanguinarine in an extraction solvent. The extraction treatment can be performed under normal pressure or under pressure. The extraction temperature is in the range of 0 ° C. to 150 ° C., preferably normal temperature to 120 ° C., and the extraction time is 1 minute to 24 hours, preferably 1 minute to 3 hours. The solvent used for the extraction is not particularly limited, but water, acid bases such as hydrochloric acid aqueous solution, sulfuric acid aqueous solution, acetic acid, sodium hydroxide aqueous solution, alcohols such as methanol, ethanol, isopropanol, polyethylene glycol, glycerin, sorbitol, acetone, dimethyl Examples include ketones such as ketone, ethyl acetate, toluene, diethyl ether, methylene chloride, dimethyl sulfoxide, chloroform, hexane, and the like, and mixtures thereof. Among these, water, dilute hydrochloric acid, acetic acid, alcohols, or a mixture thereof is desirable from the viewpoint of ease of handling and safety as a growth promoter. Although it does not specifically limit as extraction processing, Specifically, solvent extraction processing, subcritical water extraction processing, heat extraction processing, pressure extraction processing, pressure hot water extraction processing, extraction processing by a Soxhlet extraction device, supercritical extraction Processing, ultrasonic extraction processing, extraction processing by enzymatic decomposition, and the like can be applied.

The solvent extraction treatment refers to an extraction treatment performed by immersing a plant material in an appropriate solvent as described above and leaving it for a certain period of time. As processing temperature, it can heat and process to room temperature or less than the boiling point of a solvent. It is also possible to promote extraction by stirring the solvent during the immersion or pressurizing the plant material. In the solvent extract according to the embodiment of the present invention, an extraction target such as a bamboo rush or the like, a leaf, a stem or the like is added to a normal temperature ethanol solution for 10 minutes to 24 hours, preferably 30 minutes to 6 hours, more preferably 1 hour. Obtained by soaking for ~ 3 hours. Further, an extraction target such as a bamboo rush is immersed in dilute hydrochloric acid heated to 50 ° C. to 70 ° C. for 10 minutes to 24 hours, preferably 30 minutes to 6 hours, more preferably 1 hour to 3 hours. Can also be obtained.

Thermal extraction treatment refers to extraction treatment performed using a hydrous solvent at 50 ° C or higher. The heat extract according to the embodiment of the present invention is obtained by contacting or immersing an extraction target such as a bamboo rush or the like in a hydrous solvent at 50 ° C. or higher for 3 minutes to 24 hours, preferably 3 minutes to It is obtained by heating or boiling for 3 hours, more preferably 5 to 60 minutes.

The subcritical water extraction treatment refers to an extraction treatment performed using a solvent in a subcritical state at a temperature and pressure lower than the critical point (water critical point is 22 MPa, 374 ° C.). The subcritical water extract according to the embodiment of the present invention is an aqueous solvent or a mixed solvent of water and a hydrophilic solvent such as methanol and acetic acid under a subcritical state, and is used for seeds such as bamboo rush, leaves and stems. It is obtained by subjecting the extraction target to extraction treatment for 1 minute to 24 hours, preferably 1 minute to 60 minutes, more preferably 3 minutes to 10 minutes. As subcritical conditions, a pressure of 0.2 MPa to 15 MPa and a temperature of 100 ° C. to 200 ° C. are preferable.

When a plant extract is obtained from a plant containing sanguinarine such as bamboo rush and celandine by the above method, a living body or a dried body can be used as a plant material. However, it is thought that if the drying is insufficient or if it is left for a long time after drying, it sometimes becomes moldy, and the stability of the extracted sanguinarine may be reduced. A significant decrease has already been confirmed when 7 days have passed since the collection. Therefore, it is preferable to use the extraction process early even when dried.

Further, the plant extract obtained as described above is obtained in a liquid state extracted in an extraction solvent, and may be neutralized by adding a base or an acid to the solvent. Moreover, it is good also as a liquid which processed the plant extract using the vacuum concentration method or the gel adsorption method, and raised the sanguinarine concentration. Furthermore, the plant extract can be processed into a powder form by using a spray dryer or a vacuum dryer. In addition, the extract processed into a powder form can be dissolved in a solvent other than the extraction solvent to facilitate the handling of the plant extract and to enhance the storage stability. In addition, if you want to increase the purity of sanguinarine in plant extracts, purify by adsorption purification with ion exchange resin, silica gel, activated carbon, etc., column chromatography, recrystallization, etc. to extract plants containing high-purity sanguinarine. You can get things.

As the plant growth promoter of the present invention, the plant extract obtained as described above can be used as it is, but it does not lose the activity of the active ingredient sanguinarine and does not adversely affect the plant to be applied. In addition, what performed the secondary process to the plant extract may be used. Further, not only plant extracts but also sanguinarine obtained by chemical synthesis or sanguinarine obtained by microbial fermentation may be used as the growth promoter of the present invention. Moreover, the growth promoter of the present invention is not limited to liquids, and may be processed into powders, granules, suspensions and the like by conventional methods.

The active ingredient content in the growth promoter of the present invention, that is, the content of sanguinarine is not particularly limited, but the sanguinarine content is 10 ppm or more so that the growth promoter of the present invention can be diluted and used. It is preferable that it is 100 ppm or more. In addition, the application amount per time of the growth promoter of the present invention depends on the weight of the plant, but if applied to a normal edible plant seedling or soil, the content of sanguinarine per 1 m ² sprayed area 0.001 mg to 50 mg is preferable, 0.005 mg to 10 mg is more preferable, and 0.01 mg to 5 mg is further preferable. On the other hand, when applying the growth promoter of the present invention to a culture medium for hydroponics, there is almost no outflow of components compared to the case where it is applied to seedlings, soil, etc., so that it can be applied more efficiently. it can. Specifically, the sanguinarine content in the culture solution is preferably 0.001 ppm to 5 ppm, more preferably 0.01 ppm to 1 ppm, and even more preferably 0.02 ppm to 0.5 ppm. Moreover, it is also possible to apply the growth promoter of this invention with respect to a hydroponics plant by the method similar to the application to the soil culture plant mentioned above. Even in such a low concentration application, the growth promoter of the present invention can sufficiently impart a growth promoting effect to the target plant and can be improved in terms of quality. The application of the growth promoter of the present invention to the plant body can be sufficiently imparted to the plant to be protected only by performing it once at an early stage throughout the entire cultivation period of the plant body. However, it can be applied multiple times in order to enhance the effect sufficiently.

Plant growth includes plant height, above-ground weight, root weight, total weight, harvest weight, harvest size, number of seeds or seed weight. Further, the quality of the plant includes contents related to appearance, taste, and other functional components. The improvement of plant growth means that the state of growth of plants and crops is judged to be in a better state than the plants in the control plot. Similarly, the improvement in plant quality means that the appearance and taste of plants and crops, other functional components, etc. are judged to be in a better state than the plants in the control plot. Further, the object of plant growth improvement is not limited to the entire plant body or crop, but may be at least one of plant organs such as flowers, leaves, fruits, stems, and roots.

The plant species to be protected as a target for imparting the growth promoting effect as described above may be either a dicotyledonous plant or a monocotyledonous plant, and is not particularly limited, but examples thereof include tomato, eggplant, pepper, capsicum, potato Solanaceae plants, celery family plants such as carrots, celery, mishimasako, red crustaceae plants such as beets and spinach, oleaceae plants such as leaf lettuce, garlic, lettuce, burdock, gerbera, soybeans, peas, licorice, alfalfa, sweet peas, etc. Legumes, lilies such as leeks, onions, garlic, tulips, roses such as strawberries, roses, apples, peaches, pears, cucurbits such as watermelon, melon, cucumber, convolvulaceae such as sweet potatoes, Camellia family plants such as tea and camellia, Cypress family such as cedar and cypress , Eucalyptus, etc., olives, etc., citrus, lemon, etc., grapes, grapes, perilla, basil, mint, rosemary, sage, etc., rice , Gramineae plants such as corn, buckwheat, barley, ryegrass, Brassicaceae plants such as Komatsuna, Chingensai, broccoli, cabbage, Bacolaceae plants such as banana, Urushiaceae plants such as mango, Papidae plants, and other tropical Examples include plants.

In addition, a spreading agent, a surfactant, a water-soluble polymer, a lubricant, an antioxidant, a preservative, and the like may be added to the growth promoter of the present invention. Moreover, you may aim at a synergistic effect by adding what has disinfection and a pest action. Thus, the plant growth promoter of the present invention contains not only a plant extract containing sanguinarine or sanguinarine alone, but also effective as sanguinarine, depending on the market transaction status, storage status, and usage status. Changes can be made as long as they do not lose their gender.

Regarding the method of applying the growth promoter of the present invention, when the growth promoter is a powder or granules, it is sprayed directly or dissolved in a solvent such as water and diluted to a predetermined concentration, and then sprayed, sprayed, injected Alternatively, it can be applied by irrigation or the like. When the growth promoter of the present invention is a liquid or a suspension, it can be applied directly, or diluted to a predetermined concentration and applied by spraying, spraying, pouring or irrigation. Further, the growth promoter of the present invention is applied to the root of the plant body, soil, culture medium for hydroponics, etc., or plant organs of the plant to be protected (flowers, leaves, fruits, stems or roots). Etc.).

As shown in the Examples described below, the growth promoting agent of the present invention is applied to the applied plant and its seeds before the harvest of the plant body and its seeds to impart a growth promoting effect to the applied plant and its seeds. Thus, since the growth promoting effect can be imparted only by applying the growth promoter of the present invention, the yield of the plant body and its grains can be improved by a simple process. Moreover, about the time which applies the growth promoter of this invention, the early stage of the cultivation period of a plant body, for example, during the seedling raising period etc., are preferable.

Examples of the present invention will be described below, but the present invention is not limited to these examples.

[Production of bamboo rush extract 1]
Fruited from summer to autumn, 1 kg of mature bamboo shoots were collected and allowed to air dry overnight in a well-ventilated place. Bamboo rush fruit and seeds therein are rich in sanguinarine. On the next day, 200 g of strawberry fruit (including seeds) was immersed in 5 L of diluted hydrochloric acid (0.1 N) adjusted to 60 ° C. Furthermore, the fruit was pressed from above with a wooden stick, and extraction was promoted while compressing the fruit and seeds. Thereafter, the temperature holding at 60 ° C. was released, and the mixture was left for 1 hour. After standing, dilute hydrochloric acid containing fruits and seeds was filtered with a filter paper having a diameter of 60 cm, and the filtrate was collected to obtain a first crude extract of bamboo rush fruits and seeds.

The resulting residue is again immersed in 5 L of dilute hydrochloric acid (0.1 N) adjusted to 60 ° C., and the fruit and seed residue are pressed from above with a wooden stick in the same manner as described above to compress the fruit and seed. Urged extraction while letting. The temperature holding at 60 ° C. was released and the mixture was allowed to stand for 1 hour, and the filtrate was collected by filtering dilute hydrochloric acid containing fruits and seeds with a filter paper having a diameter of 60 cm to obtain a second crude extract of bamboo shoots fruits and seeds. And what combined the 1st rough extract and the 2nd rough extract was made into the rough extract of a bamboo rush fruit and a seed.

Next, the crude extract of bamboo rush fruit and seeds was purified. Spherical silica gel for purification (Wakogel (registered trademark) 50C18, product of Wako Pure Chemical Industries, Ltd.) was activated by treatment with 95% ethanol, and the alcohol was then replaced by washing with water. The silica gel was placed on one side on a filter paper set in a funnel, and the preparation of the filter device was completed. From above the silica gel, the above-mentioned crude extract of bamboo rush fruit and seeds was poured, and sanguinarine as the target substance was adsorbed on the silica gel as a main component. And after wash | cleaning this silica gel with a tap water, the adsorbate was eluted with 95% ethanol, and the extract of the bamboo rush fruit and seeds of about 700 mL was obtained. Using HPLC, the concentration of sanguinarine contained in the obtained extract was measured. As a result, it was confirmed that this extract contains about 1,500 ppm of bamboo rush fruit and seed-derived sanguinarine. . The obtained extract is hereinafter referred to as “dilute hydrochloric acid extract”. In Examples 4 to 15 below, this “dilute hydrochloric acid extract” was diluted and used for each test.

[Production of bamboo rush extract 2]
The mature bamboo shoots of bamboo rush were dried and crushed. 97 g of this pulverized product was placed in a beaker and immersed in 1000 mL of a 40% ethanol solution. The mixture was left at room temperature for about 2 hours, and the solution in the beaker was occasionally stirred to facilitate extraction. After standing, it was filtered through a filter paper (NO.2, manufactured by Toyo Filter Paper Co., Ltd.) to recover about 820 mL of filtrate. Using HPLC, the concentration of sanguinarine contained in the obtained filtrate, that is, the ethanol extract was measured. The extract contained about 1,000 ppm of bamboo rush fruit and seed-derived sanguinarine. Was confirmed. The obtained extract is hereinafter referred to as “ethanol extract”. In Examples 16 to 18 below, this “ethanol extract” was diluted and used for each test.

[Production of bamboo rush extract 3]
1 kg of mature bamboo shoots were collected and allowed to air dry overnight in a well-ventilated place. On the next day, boiled (heated) extraction and subcritical water extraction were performed on the strawberry fruit (including seeds). As the solvent, 500 mL of a mixture of water 10: methanol 9: acetic acid 1 (volume ratio) was used for both boiling extraction and subcritical water extraction. In boiling extraction, 20 g of fruits (including seeds) were extracted under normal pressure and boiling time of 30 minutes. On the other hand, subcritical water extraction is performed on 20 g of fruit (including seeds) at a temperature of 100 ° C., a pressure of 3 MPa and a treatment time of 5 minutes, a condition in which only the temperature is changed to 140 ° C. The extraction process was performed under a total of three extraction conditions.

The concentration of sanguinarine contained in the obtained boiling extract and each subcritical water extract was measured using HPLC (UPLC (registered trademark), product of Japan Waters) (UPLC). The results are shown in Table 1. The numerical values described in each extraction process in Table 1 indicate the peak areas of the components corresponding to sanguinarine contained in each extract in HPLC measurement.

As shown in Table 1, it was confirmed that the peak area of sanguinarine was 400 in the boiling extraction process, but 1300 to 1400 in the subcritical water extraction process. As a result, it was found that sanguinarine was extracted at a concentration three times or higher in subcritical water extraction compared to boiling extraction, and high-efficiency extraction could be realized. From the results of Examples 1 to 3, it was confirmed that sanguinarine can be extracted from bamboo rush by various extraction methods.

[Evaluation of growth promoting effect 1: Tomato (Solanumaceae)]
A seedling of tomato (variety: Momotaro) was prepared, and two seedlings were planted in a 25 L planter and cultivated in a greenhouse. The donor soil used was light-colored black soil, applied with 2 t / 10a of bark compost as compost and 150 kg / 10a of lime lime as soil improvement material. As for fertilizer application conditions, NPK = 3-4.5-3kg / 10a for the original fertilizer and NPK = 3-4.5-3kg / 10a for the additional fertilizer are applied 10 times during the cultivation period. did. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 45 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, a total of three irrigation treatments were performed on the 14th, 28th and 61st days after planting, and the amount of liquid used was 0.5 L / m ² respectively. Tomato fruits were harvested 70 days after planting. In addition, the test (control group) which grows a tomato on the cultivation environment conditions similar to the above was performed except not adding sample liquid.

Table 2 shows the results of measuring the number and fruit weight of the tomato fruits in each of the bunches in the control group and the test group, and the plant height and the above-ground weight of the plant body. Table 3 shows the results of measuring the sugar content, the concentration of nitric acid, and vitamin C contained in the tomato fruit of each fruit bunch in the control group and the test group. The numerical value in parentheses in the test group indicates the ratio to the numerical value in the control group.

From the measurement results shown in Table 2, it was confirmed that the tomato in the test group to which the extract containing sanguinarine was added had better yield and the growth of stems and leaves than the tomato in the control group. Furthermore, as shown in Table 3, the tomato fruit of the test group to which the extract was added was superior in sugar content and nitric acid concentration to about half that of the tomato fruit of the control group, indicating superior taste quality. It was done. In general, nitric acid contained in tomato fruits may cause health damage in the body after ingestion, so that it is preferable to have a low nitric acid concentration. In the present Example, it turned out that the highly safe tomato fruit which reduced the nitric acid concentration remarkably is obtained by adding the extract containing sanguinarine. In the test area, it was confirmed that tomato fruit having a high vitamin C concentration contained in the fruit and excellent in health-related functions was obtained. In this way, by irrigating a plant body with an extract containing sanguinarine, the yield of fruits is increased, the growth of stems and leaves is promoted, and fruits with excellent quality such as taste and safety are obtained. It was confirmed that

[Evaluation of growth promoting effect 2: Cucumber (Cucurbitaceae)]
Cucumber (variety: Hokushin) seedlings were prepared, and two seedlings were planted in a 25 L planter and cultivated in a greenhouse. The donor soil used was light-colored black soil, applied with 2 t / 10a of bark compost as compost and 150 kg / 10a of lime lime as soil improvement material. As fertilizer application conditions, NPK = 8-12-8 kg / 10a was used for the original fertilizer, and NPK = 3-4.5-3 kg / 10a was similarly applied 10 times during the cultivation period. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 45 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, a total of four irrigation treatments were performed on the 11th, 26th, 31st and 47th days after planting, and the amount of liquid used was 0.5 L / m ² respectively. Cucumber fruits were harvested in 12 portions within the period from the 31st day to the 54th day after planting. In addition, except not adding an extract, the test (control group) which grows a cucumber on the cultivation environment conditions similar to the above was done.

Table 4 shows the results of measurement of the number of fruits, total fruit weight, fruit weight per fruit, plant height and above-ground weight of the cucumber fruits in the control group and the test group. Table 5 shows the results of measuring the average sugar content of the cucumber fruits and the appearance quality of the cucumber fruits in the control group and the test group. The numerical value in parentheses in the test group indicates the ratio to the numerical value in the control group.

From the measurement results shown in Table 4, it was confirmed that the cucumber in the test group to which the extract containing sanguinarine was added had better yield and stem / leaf growth than the cucumber in the control group. Furthermore, as shown in Table 5, it was shown that the cucumber fruit in the test group to which the extract was added was superior in sugar content and more delicious than the cucumber fruit in the control group. In the control plot, normal fruits with a straight shape could not be harvested, but only abnormal fruits such as bent fruits, tapered fruits and constricted fruits were harvested. It was shown that by adding an extract containing sanguinarine, an excellent quality cucumber fruit was obtained in appearance.

[Evaluation of growth promoting effect 3: Sweet corn (Poaceae)]
Sweet corn (variety: gold rush) seedlings were prepared, and two seedlings were planted in an 18 L planter and cultivated in a greenhouse. The donor soil used was light-colored black soil, applied with 2 t / 10a of bark compost as compost and 150 kg / 10a of lime lime as soil improvement material. As fertilization conditions, the original fertilizer was NPK = 26-39-26 kg / 10a, and no additional fertilization was performed. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 45 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, irrigation treatment was performed three times on the 14th day, 28th day, and 61st day after planting, and the amount of liquid used was 0.5 L / m ² , respectively. Corn grains were harvested on day 68 after planting. In addition, except not adding an extract, the test (control group) which grows a cucumber on the cultivation environment conditions similar to the above was done.

Table 6 shows the results of measuring the number, fruit weight, plant height, leaf length, and above-ground weight of the corn grains in the control group and the test group. The numerical value in parentheses in the test group indicates the ratio to the numerical value in the control group.

From the measurement results shown in Table 6, it was confirmed that the corn of the test group to which the extract containing sanguinarine was added had better yield and stem / leaf growth than the control corn. In particular, the corn fruit weight in the test plot was 180%, nearly twice that of the control plot, indicating a significant yield improvement effect.

[Evaluation of growth promoting effect 4: Soybean (Fabaceae)]
Soybean (variety: Fusami Midori) seedlings were prepared, and two seedlings were planted in an 18 L planter and cultivated in a greenhouse. The donor soil used was light-colored black soil, applied with 0.5 t / 10a of bark compost as compost and 150 kg / 10a of limestone lime as soil improvement material. As fertilization conditions, NPK = 4-6-4 kg / 10a was used for the original fertilizer and NPK = 6-9-6 kg / 10a was applied once during the cultivation period. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 45 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, a total of three irrigation treatments were performed on the 14th, 28th and 61st days after planting, and the amount of liquid used was 0.5 L / m ² respectively. Soybeans were harvested on day 68 after planting. In addition, except not adding an extract, the test (control group) which grows soybean on the same cultivation environment conditions as the above was done.

Table 7 shows the number of soybean pods harvested in the control and test plots, 1 pod (one with 1 bean in the pod), 2 pods (2 in bean) 3 shows the results of measuring the number of cocoons (the number of beans in the cocoons is 3), the culm weight, the plant height of the plant body and the above-ground part weight. The numerical value in parentheses in the test group indicates the ratio to the numerical value in the control group.

From the measurement results shown in Table 7, it was confirmed that the soybeans in the test group to which the extract containing sanguinarine was added had better yield and the growth of stems and leaves than the soybeans in the control group. In particular, the number of soybeans in the test area was slightly less than the number of soybeans in the control area, but the number of soybeans in the 3 area (the number of beans in the tree was 3) was twice as large. It was shown that there is an effect of improving the yield.

[Evaluation of growth promotion effect 5: Potato (Solanumaceae)]
Potato seedlings (variety: Baron) were prepared, two seedlings were planted in a 25 L planter and cultivated outdoors. The donor soil used was light-colored black soil, applied with 1 t / 10a of bark compost as compost, and 150 kg / 10a of bitter lime as soil improvement material. As the fertilizer application conditions, the original fertilizer was NPK = 18-26-20 kg / 10a, and no additional fertilization was performed. As the cultivation environment conditions, the outdoor day maximum temperature during the cultivation period was 30 to 35 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, irrigation treatment was performed on the 88th day after planting so that the amount of the extract used was 0.5 L / m ² . Thereafter, soybeans were harvested 104 days after the planting. Moreover, the test in the soil which performed the green manure penetration process on the same conditions was done. In addition, the test (control group) which grows a potato on the cultivation environment conditions similar to the above was performed except not adding an extract.

Table 8 shows the results of measuring the number and weight of harvested potatoes in the control plot and test plot. The numerical value in parentheses in the test group indicates the ratio to the numerical value in the control group.

From the measurement results shown in Table 8, it was confirmed that the yield of the potato in the test group to which the extract containing sanguinarine was added increased compared to the potato in the control group. In addition, when comparing the results with green manure in the control plot and the results without green manure in the test plot, the effect of adding the extract is higher than the effect of green manure penetration. It has been shown.

[Evaluation of growth promotion effect 6: cabbage (Brassicaceae)]
Cabbage (variety: Kinharu) seedlings were prepared, and two seedlings were planted in 18L planters and cultivated in a greenhouse. The donor soil used was light-colored black soil, applied with 3 t / 10a of bark compost as compost and 150 kg / 10a of lime lime as soil improvement material. As fertilizer application conditions, NPK = 16-24-6 kg / 10a was applied to the original fertilizer and NPK = 8-12-8 kg / 10a was applied twice during the cultivation period. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 40 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, a total of three irrigation treatments were performed on the 14th, 28th and 61st days after planting, and the amount of liquid used was 0.5 L / m ² respectively. Cabbage was harvested 70 days after planting. In addition, except not adding an extract, the test (control group) which grows cabbage on the cultivation environment conditions similar to the above was done.

Table 9 shows the results of measuring the total weight and adjusted weight of the cabbage in the control group and the test group. The adjusted weight is the weight after adjusting for shipping the cabbage. Moreover, the numerical value in the parenthesis in the test group shows the ratio to the numerical value in the control group.

From the measurement results shown in Table 9, it was confirmed that the cabbage in the test area to which the extract containing sanguinarine was added was heavier than the cabbage in the control area and was superior in yield. In addition, a lottery was found in the control cabbage, but not in the test cabbage. Thus, it was confirmed that by irrigating a plant body with an extract containing sanguinarine, the yield can be increased and the lottery can be suppressed, and a crop with excellent quality can be obtained.

[Evaluation of growth promotion effect 7: Leaf lettuce (Asteraceae)]
Leaf lettuce (variety: redfire) seedlings were prepared, and two seedlings were planted in an 18 L planter and cultivated in a greenhouse. The donor soil used was light-colored black soil, applied with 2 t / 10a of bark compost as compost and 150 kg / 10a of lime lime as soil improvement material. As the fertilizer application conditions, the original fertilizer was NPK = 20-30-20 kg / 10a, and no additional fertilization was performed. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 40 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, a total of three irrigation treatments were performed on the 14th, 28th and 61st days after planting, and the amount of liquid used was 0.5 L / m ² respectively. Leaf lettuce was harvested 70 days after planting. In addition, except not adding an extract, the test (control group) which grows leaf lettuce on the same cultivation environment conditions as the above was done.

Table 10 shows the results of measuring the total weight and adjusted weight of leaf lettuce in the control group and the test group. The adjusted weight is the weight after adjusting for shipping leaf lettuce. Moreover, the numerical value in the parenthesis in the test group shows the ratio to the numerical value in the control group.

From the measurement results shown in Table 10, it was confirmed that the leaf lettuce in the test group to which the extract containing sanguinarine was added was superior in yield to the leaf lettuce in the control group. In addition, lime deficiency was found in the lottery and the heart lobe in the leaf lettuce in the control group, but not in the leaf lettuce in the test group. In this way, by irrigating the plant body with an extract containing sanguinarine, it is confirmed that the yield can be increased and the yield and the lime deficiency can be suppressed, and a crop with excellent quality can be obtained. It was.

[Evaluation of growth promotion effect 8: Paddy rice (Gramineae)]
A seedling of paddy rice (variety: Kaori Aichi) was prepared, planted in a Wagner pot 1 / 2000a, and cultivated in a greenhouse. The donated soil was gray lowland soil, no compost was used, and 60 kg / 10a of limestone lime was applied as a soil improvement material. As fertilization conditions, NPK = 3-1-2 kg / 10a was applied to the original fertilizer, and NPK = 2-0.5-1 kg / 10a was applied to the panicle. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 45 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, a total of three irrigation treatments were performed on the 6th, 42nd, and 83rd days from rice planting, and the amount of liquid used was 0.5 L / m ² , respectively. Harvested rice on the 135th day after rice planting. In addition, except not adding an extract, the test (control group) which grows a paddy rice on the same cultivation environmental conditions as the above was done.

Table 11 shows the results of measuring the yield of rice and the weight of the stock in the control and test plots. The numerical value in parentheses in the test group indicates the ratio to the numerical value in the control group.

From the measurement results shown in Table 11, it was confirmed that the rice in the test group to which the extract containing sanguinarine was added was superior in yield and plant growth than the rice in the control group. In addition, it was confirmed that the rice in the test area had a lower ratio of waste rice than the rice in the control area, and a harvest that was excellent in the quality of the harvest was obtained.

[Examination of processing method 1]
Komatsuna (Brassicaceae, cultivar: Wakami) seeds were prepared, 5 seeds were sown in a 4L planter and cultivated in a greenhouse. The donor soil used was light-colored black soil, applied with 1 t / 10a of bark compost as compost, and 150 kg / 10a of bitter lime as soil improvement material. As the fertilizer application conditions, the original fertilizer was NPK = 6-9-6 kg / 10a, and no additional fertilization was performed. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 40 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, irrigation was performed so that the amount of the extract used at the time of sowing was 0.5 L / m ^2, and komatsuna was harvested on the 56th day after sowing (test group 1). In addition, the irrigation treatment of the sample solution was not performed at the time of sowing, but on the 9th day (study group 2) after sowing, the 17th day (see test group 3) after sowing, and the 9th and 17th days after sowing (test group). A test for cultivating Komatsuna was performed under the same conditions as described above except that 4) and no irrigation treatment of the extract (control group) were performed.

Table 12 shows the results of measuring the plant height, the above-ground weight and the root weight of the harvested Komatsuna in the control plot and the test plots 1 to 4. The numerical value in parentheses in each test group indicates a ratio to the numerical value in the control group.

From the measurement results shown in Table 12, it was confirmed that the Komatsuna in the test groups 1 to 4 to which the extract containing sanguinarine was added had better yield and plant growth than the Komatsuna in the control group. It was. Further, according to the results of the test groups 1 to 4, it was found that a sufficient growth effect can be imparted by only one addition treatment at the time of sowing. Thus, it was shown that the growth of the plant body can be promoted and the yield can be increased only by irrigating the extract containing sanguinarine early in the cultivation period.

[Examination of processing method 2]
Spinach seeds (Aceraceae, cultivar: okame) were prepared, and 5 seeds were sown in a 6L planter and cultivated in a greenhouse. The donor soil used was light-colored black soil, applied with 1 t / 10a of bark compost as compost, and 150 kg / 10a of bitter lime as soil improvement material. As the fertilizer application conditions, the original fertilizer was NPK = 6-9-6 kg / 10a, and no additional fertilization was performed. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 30 to 40 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, irrigation treatment was performed so that the amount of the sample solution used was 0.5 L / m ² at the time of seeding, and spinach was harvested on the 56th day after seeding (test group 1). In addition, the irrigation treatment of the sample solution was not performed at the time of sowing, but on the 9th day (study group 2) after sowing, the 17th day (see test group 3) after sowing, and the 9th and 17th days after sowing (test group). A test for cultivating spinach was performed under the same conditions as described above except that 4) and no sample solution irrigation treatment (control group) were used.

Table 13 shows the results of measuring the plant height and the above-ground weight of the harvested spinach in the control plot and the test plots 1 to 4. The numerical value in parentheses in each test group indicates a ratio to the numerical value in the control group.

From the measurement results shown in Table 13, it was confirmed that the spinach in the test groups 1 to 4 to which the extract containing sanguinarine was added had better yield and plant growth than the spinach in the control group. It was. Further, according to the results of the test sections 1 to 4, it is found that the effect is enhanced by the addition treatment at the early stage of cultivation, and a sufficient growth effect can be imparted by only one addition treatment at the time of sowing or early sowing. It was. Thus, it was shown that the growth of the plant body can be promoted and the yield can be increased only by irrigating the extract containing sanguinarine early in the cultivation period.

[Examination of processing method 3]
Komatsuna (Brassicaceae, variety: Wakami) seeds were prepared, and 20 seeds were sown in a 500 mL Neu Bayer pot and cultivated in a greenhouse. Donated soil was light-colored black soil, and no compost or soil improvement material was used. As the fertilizer application conditions, the original fertilizer was NPK = 7-7-7 kg / 10a, and no additional fertilization was performed. As the cultivation environment condition, the indoor daily maximum temperature during the cultivation period was 20 to 30 ° C., and watering was performed every time the soil surface was dried. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1 ppm. Using this sample solution, irrigation was performed so that the amount of the sample solution used was 0.5 L / m ² 7 days after seeding, and komatsuna was harvested 48 days after seeding (test group 1). Moreover, the sample liquid (test group 2) which adjusted the containing sanguinarine concentration to 1.5 ppm, the sample liquid (test group 3) which adjusted the containing sanguinarine concentration to 2 ppm, and the sample liquid (test which adjusted the containing sanguinarine concentration to 2.5 ppm) A test for cultivating Komatsuna was performed under the same conditions as described above except that the ward 4) was used. Furthermore, the test which grows a komatsuna on the same conditions as the above without adding a sample solution was done (control group).

Table 14 shows the plant height, the ratio of the above-ground weight and the root weight (%) and the germination rate (%) of Komatsuna in the test groups 1 to 4 with respect to the values of the control group.

From the results shown in Table 14, the growth was better in all the test plots than in the control plot. Moreover, when it looked at the sanguinarine density | concentration in a sample liquid, it turned out that the growth promotion effect can be provided from 1 ppm and an effect can be given to a plant body by application of a low concentration. From the results of this example and the above-described examples, it was found that the plant growth can be promoted and the yield can be improved only by applying a low concentration of sanguinarine to the plant body early in the cultivation period. Therefore, since it can be applied to pot seedlings before planting, the amount of plant growth promoter containing sanguinarine is less than that applied to fields after planting, and the labor of application is also reduced. Therefore, the growth promoting effect can be easily imparted to the plant at low cost.

[Evaluation of growth promotion effect in field test 1: paddy rice]
A seedling of paddy rice (variety: Koshihikari) was prepared and cultivated by planting it in a test field of 50 m ² in Gotemba, Shizuoka Prefecture. The soil was gray lowland soil, compost was not used, and 120 kg / 10a was applied as a soil improvement material. As fertilization conditions, the original fertilizer is NPK = 3.5-9-4 kg / 10a, and the hot manure is NPK = 3-0-3 kg / 10a on the 80th day after rice planting. On the 90th day, NPK = 1-0-1 kg / 10a was applied. A diluted hydrochloric acid extract (contained sanguinarine concentration: about 1500 ppm) obtained in Example 1 was diluted with water to prepare a sample solution adjusted so that the sanguinarine concentration was 1.5 ppm. Using this sample solution, irrigation was performed on a part of the test field so that the amount of the sample solution used was 0.5 L / m ^{2 on the} 89th day after rice planting, and the ears were harvested on the 138th day after rice planting. (Test Zone 1). In addition, a test for cultivating paddy rice under the same conditions as described above was carried out except that the sample solution was irrigated on day 108 from the rice planting (test group 2) and the sample solution was not irrigated (control group).

Table 15 shows the results of calculating the ratio (%) of the harvested amount of rice and the weight of the stock in the test plots 1 to 2 with respect to the values in the control plot. Table 16 shows the taste analysis results of the harvested rice in the control plot and test plots 1-2, and Table 17 shows the grain discrimination results.

From the measurement results shown in Table 15, it was confirmed that in the field test as well, in the same manner as in the greenhouse test, by adding an extract containing sanguinarine, the yield and plant growth were excellent. Moreover, since the amount of waste rice reduced, it turned out that the cultivation early stage is preferable for the addition process time to a plant body as shown by the above-mentioned Example. Furthermore, when the target taste value of rice is 6.8% or less of protein, 20% or less of amylose, 20 mg or less of fatty acid, and a taste value of 80 or more, as shown in Table 16, the taste analysis value is tested more than the control group. It was confirmed that the ward was superior and the test ward showed superior quality in terms of taste quality. In addition, when the harvested rice was analyzed using a grain discriminator (Satake Co., Ltd.), as shown in Table 17, the test plot had more sized rice than the control plot, and milk white rice and immature rice It has been confirmed that incomplete rice such as rice is reduced and the appearance quality is excellent. In addition, also in the taste quality and the appearance quality, since the test group 1 shows a better value than the test group 2, the plant is treated at an early stage for the purpose of improving the quality. It turned out to be preferable.

[Evaluation of growth promotion effect in field test 2: Chingensai]
A seedling of chingensai (variety: Natsu Aoi) was prepared and planted in a test field of 20 m ² in Omaezaki City, Shizuoka Prefecture, and cultivated. The soil was immature sand dune, and was used by applying cattle manure 2t / 10a as compost, and Selca 150kg / 10a and silicate potassium 50kg / 10a as soil improvers. As fertilizer application conditions, NPK = 10−10−28 kg / 10a was used as the original fertilizer, and no additional fertilizer was used. The ethanol extract (contained sanguinarine concentration: about 1000 ppm) obtained in Example 2 was diluted with water to prepare a sample solution adjusted so that the contained sanguinarine concentration was 1.0 ppm. Using this sample solution, the seedlings on the day before planting were irrigated so that the amount of the sample solution used was 0.5 L / m ^2, and the first crop was harvested on the 31st day after planting. (Test Zone 1). Further, in the same manner as described above, the second crop of chingensai (test group 2) and the third crop of chingensai (test group 3) were harvested. In addition, as a control group corresponding to each test group, a test for cultivating chingensai without adding the sample solution was performed (control group 1 to 3).

Table 18 shows the measurement results of plant height, total weight, and adjustment weight of each control group and each test group. Moreover, about the control group 3 and the test group 3, the measurement result of the nitric acid density | concentration contained in a Chingensai is shown. In addition, adjustment weight is the weight after adjusting in order to ship Chinggensai. Moreover, the numerical value in the parenthesis in each test group shows the ratio to the value of the corresponding control group.

From the measurement results shown in Table 18, it was confirmed that by adding an extract containing sanguinarine, the yield and plant growth were excellent. In addition, compared to the control group, the amount of nitric acid in the plant body of the test group was significantly reduced, indicating that the quality related to taste and safety was also improved.

[Evaluation of growth-promoting effect in field test 3: scallion]
A seedling of scallion (variety: Kanayama-ji) was prepared and planted in a test field of 25 m ² in Shizuoka City, Shizuoka Prefecture, and cultivated. The soil was immature sand dune, and was used by applying cattle manure 2t / 10a as compost and Selca 100kg / 10a as soil improvement material. As fertilization conditions, NPK = 14-7-7 kg / 10a was used as the original fertilizer, and NPK = 6-3-3 kg / 10a was applied as the fertilizer. The ethanol extract (contained sanguinarine concentration: about 1000 ppm) obtained in Example 2 was diluted with water to prepare a sample solution adjusted so that the contained sanguinarine concentration was 1.0 ppm. Using this sample solution, the seedlings on the 19th day after planting were irrigated so that the amount of the sample solution used was 0.5 L / m ^2, and onions were harvested 44 days after planting. In addition, the test (control group) which grows a leek on the cultivation environment conditions similar to the above was performed except not adding a sample solution.

Table 19 shows the measurement results of plant height, total weight, and adjustment weight of each control group and each test group. In addition, as items for evaluating quality, the presence or absence of tip wilt of harvested scallions and the measurement results of vitamin C concentration in scallions are shown. These measurements are the average of 55 harvested scallions. The adjustment weight is the weight after adjustment for shipping the spring onions. Moreover, the numerical value in the parenthesis in each test group shows the ratio to the value of the corresponding control group.

From the measurement results shown in Table 19, it was confirmed that by adding an extract containing sanguinarine, the yield and plant growth were excellent. In addition, compared to the control group, the plants in the test group showed no dead end plants, the vitamin C concentration in the plant body was high, and it was shown that the quality related to appearance quality and functionality was improved.

[Evaluation of growth promotion effect in field test 4: scallion]
A seedling of scallion (variety: Kanayamaji) was prepared and planted in a 50 m ² test field in Shizuoka City, Shizuoka Prefecture, and cultivated. The soil was immature sand dune, and was used by applying cattle manure 2t / 10a as compost and Selca 100kg / 10a as soil improvement material. As fertilizer application conditions, NPK = 10-6-6 kg / 10a was used as the original fertilizer, and NPK = 5-2-2 kg / 10a was applied as the fertilizer. The ethanol extract (contained sanguinarine concentration: about 1000 ppm) obtained in Example 2 was diluted with water to prepare a sample solution adjusted so that the contained sanguinarine concentration was 1.0 ppm. Using this sample solution, the seedlings on the 10th day after the planting were irrigated so that the amount of the sample solution used was 0.5 L / m ^2, and the spring onion was harvested on the 51st day after the planting. In addition, the test (control group) which grows a leek on the cultivation environment conditions similar to the above was performed except not adding a sample solution.

Table 20 shows the measurement results of plant height, total weight and adjustment weight of each control group and each test group. In addition, as items for evaluating the quality, the presence or absence of tip end death of the harvested scallions, the measurement results of vitamin C concentration and glucose concentration in the scallions are shown. These measurements are the average values of 35 harvested scallions. The adjustment weight is the weight after adjustment for shipping the spring onions. Moreover, the numerical value in the parenthesis in each test group shows the ratio to the value of the corresponding control group.

From the measurement results shown in Table 20, it was confirmed that the yield and plant growth were excellent by adding an extract containing sanguinarine. In addition, compared with the control group, the scallion in the test group has a low rate of tip death, the vitamin C concentration and the glucose concentration in the plant body are high, and it is shown that appearance quality and quality related to functionality such as taste and health are improved. It was.

The plant growth promoter of the present invention has clear active ingredients and can be applied to plants that require safety, such as edible plants. Furthermore, the plant growth promotion effect can be imparted to a target plant.

Claims (7)

1. Formula (I):
   

   

   A plant growth promoter comprising at least one of sanguinarine or a salt thereof represented by the formula:
2. The plant growth promoter according to claim 1, wherein the active ingredient is a plant-derived material containing at least one of the sanguinarine or a salt thereof.
3. The plant growth promoter according to claim 2, wherein the plant-derived material is a bamboo rush-derived material.
4. The plant growth promoter according to claim 3, wherein the bamboo rush-derived material is a bamboo rush seed-derived material.
5. The plant-derived product is obtained by at least one extraction treatment selected from the group consisting of a solvent extraction treatment, a subcritical water extraction treatment and a heat extraction treatment on a plant containing at least one of the sanguinarine or a salt thereof. The plant growth promoter according to claim 2, which is an extract.
6. A plant growth promoting method comprising a step of applying the plant growth promoting agent according to any one of claims 1 to 5 to a plant, soil, or a culture medium for hydroponics.
7. The plant growth promotion method according to claim 6, wherein the plant, soil, or culture medium for hydroponics is a plant body during seedling, seedling culture soil, or a culture solution during seedling growth.