WO2015030443A1 - Bowl for centrifuge and centrifuge including same - Google Patents

Bowl for centrifuge and centrifuge including same Download PDF

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Publication number
WO2015030443A1
WO2015030443A1 PCT/KR2014/007879 KR2014007879W WO2015030443A1 WO 2015030443 A1 WO2015030443 A1 WO 2015030443A1 KR 2014007879 W KR2014007879 W KR 2014007879W WO 2015030443 A1 WO2015030443 A1 WO 2015030443A1
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WO
WIPO (PCT)
Prior art keywords
tissue
centrifuge
paul
container
partition wall
Prior art date
Application number
PCT/KR2014/007879
Other languages
French (fr)
Korean (ko)
Inventor
김주태
한현희
박연아
김해권
박세아
주환수
김용수
Original Assignee
주식회사 아이쎌
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Publication date
Application filed by 주식회사 아이쎌 filed Critical 주식회사 아이쎌
Publication of WO2015030443A1 publication Critical patent/WO2015030443A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B04B7/12—Inserts, e.g. armouring plates
    • B04B7/14—Inserts, e.g. armouring plates for separating walls of conical shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/0464—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with hollow or massive core in centrifuge bowl

Definitions

  • the present invention relates to a centrifuge Paul and a centrifuge including the same, and more particularly, a centrifuge Paul used in a centrifuge for separating a substance in a tissue by using a centrifugal force. It has a container that rotates in accordance with the application of the rotation force; A partition wall disposed in the accommodation part and partitioning the accommodation part into a radially inner space and an outer space, the partition having a gap spaced apart from the bottom surface of the container by a predetermined distance to move the tissue between the inner space and the outer space; And a pipe portion disposed in the inner space and configured to access the tissue. Including;
  • the bottom center of the container is provided with a depression in which the tissue is deposited
  • the partition is provided with a plurality of cuts for partitioning the tissue moving through the gap, the plurality of cuts are the circumferential direction of the partition
  • the present invention relates to a centrifuge and a centrifuge including the same, which are arranged at a predetermined interval apart from each other.
  • Centrifuges are used to separate specific cells, substances, and the like from certain tissues, such as adipose tissue.
  • the centrifuge has been widely used as a device for separating cells, substances, and the like in a tissue using centrifugal force, providing a relatively simple and easy separation thereof.
  • a predetermined bowl is used to receive a predetermined tissue and to be rotatable to perform centrifugation.
  • Such Paul is usually constructed in a cylindrical shape to facilitate rotation.
  • a predetermined substance such as collagen is separated from the adipose tissue.
  • the material may clump together and remain in the container, blocking the entrance and exit of the separated material to cause damage and malfunction of the device.
  • the container having a receiving portion for receiving a predetermined tissue is rotated in accordance with the application of rotational force;
  • a partition wall disposed in the accommodation part and partitioning the accommodation part into a radially inner space and an outer space, the partition having a gap spaced apart from the bottom surface of the container by a predetermined distance to move the tissue between the inner space and the outer space;
  • a pipe portion disposed in the inner space and configured to access the tissue.
  • the bottom center of the container is provided with a depression in which the tissue is deposited
  • the partition is provided with a plurality of cuts for partitioning the tissue moving through the gap, the plurality of cuts are the circumferential direction of the partition
  • the centrifuge Paul used in the centrifuge Paul for separating the material in the tissue by using the centrifugal force has a receiving portion for receiving a predetermined tissue.
  • a pipe portion disposed in the inner space and configured to access the tissue.
  • the bottom center of the container is provided with a depression in which the tissue is deposited
  • the partition is provided with a plurality of cuts for partitioning the tissue moving through the gap, the plurality of cuts are the circumferential direction of the partition Are spaced apart along the predetermined interval.
  • the partition wall is configured to be independent of the container.
  • the partition wall is formed in a cylindrical shape extending in the vertical direction, the lower cross-sectional area is configured to be tapered to be larger than the upper cross-sectional area.
  • each cut portion is composed of a beam connected to the lower portion of the partition wall and extending in the outward direction of the partition wall.
  • the plurality of cutouts includes at least a first cutout and a second cutout that is symmetrically disposed with respect to the first cutout about the center of the container.
  • the outer ends of the plurality of cut portions are configured to be supported in contact with at least one of the inner surface and the bottom surface of the container.
  • the cutting portion, the blade for performing the division of the tissue is formed on at least one side, the blade is configured to have a sharp end to perform the cutting.
  • At least one portion of the inner surface of the container is provided with a stopper for contacting the cutting portion to rotate the partition and the container to rotate together, wherein the stopper, at least two or more cutting portions of the plurality of cutting portions Is placed in between.
  • the pipe portion is configured such that the lower end portion is extended to the inside of the depression.
  • the apparatus further includes an entrance part disposed above the pipe part and configured to inject and outflow tissue, wherein the entrance part comprises an injection tube and an extraction tube configured to be symmetrical with each other in both lateral directions, and the injection tube and the extraction tube. It is comprised including the wall part provided in between.
  • Paul for centrifugal separator includes a partition for dividing the receiving portion in the container, the lower portion of the partition is provided with a plurality of cutting parts to be divided into the tissue moving through the gap between the partition and the container of the tissue Partitioning can be made.
  • the tissue is divided and has a small volume, and the surface area is widened, when the separation and various reactions are to be performed by using a predetermined enzyme or the like, the enlarged surface area improves the reaction efficiency and shortens the reaction time. Separation can be achieved.
  • FIG. 1 is a perspective view of a centrifuge Paul according to an embodiment of the present invention.
  • FIG. 2 is a side view of a Paul for centrifuge according to the present invention.
  • Figure 3 is a cross-sectional view showing the structure of the centrifuge Paul according to an embodiment of the present invention.
  • FIG. 4 is a plan view of a vessel of Paul for centrifuge according to an embodiment of the present invention.
  • FIG. 5 is a cross-sectional view of the container of FIG. 4 as seen from A-A.
  • FIG. 6 is a cross-sectional view of the container of FIG. 4 as seen from B-B.
  • FIG. 6 is a cross-sectional view of the container of FIG. 4 as seen from B-B.
  • Figure 7 is a perspective view showing the structure of the partition wall of the Paul for centrifuge according to an embodiment of the present invention.
  • FIG. 9 is an enlarged view of K of FIG. 7.
  • FIG. 10 is a perspective view showing another form of partition wall of the centrifuge Paul according to an embodiment of the present invention.
  • the present invention relates to a centrifuge Paul and a centrifuge including the same, and more particularly, the centrifuge Paul according to the present invention, the centrifuge Paul used in the centrifuge for separating the material in the tissue using the centrifugal force.
  • a container having a receiving portion for receiving a predetermined tissue is rotated in accordance with the application of rotational force;
  • a partition wall disposed in the accommodation part and partitioning the accommodation part into a radially inner space and an outer space, the partition having a gap spaced apart from the bottom surface of the container by a predetermined distance to move the tissue between the inner space and the outer space;
  • a pipe portion disposed in the inner space and configured to access the tissue.
  • the bottom center of the container is provided with a depression in which the tissue is deposited
  • the partition is provided with a plurality of cuts for partitioning the tissue moving through the gap, the plurality of cuts are the circumferential direction of the partition It is configured to be spaced apart at a predetermined interval along the.
  • spatially relative terms below “, “ beneath “, “ lower”, “ above “, “ upper” It can be used to easily describe the correlation of a member or component with other elements or components. Spatially relative terms are to be understood as including terms in different directions of the member in use or operation in addition to the directions shown in the figures. For example, when the member shown in the figure is reversed, “upper” may be interpreted as “lower”. The member can also be oriented in other directions, so that spatially relative terms can be interpreted according to the orientation.
  • FIG. 1 is a perspective view of a centrifuge Paul (1) according to an embodiment of the present invention
  • Figure 2 is a side view of the centrifuge Paul (1) according to the present invention
  • Figure 3 is an embodiment of the present invention Sectional drawing which shows the structure of the centrifuge Paul (1).
  • Figure 4 is a plan view of the container 100 of the centrifuge Paul 1 according to an embodiment of the present invention
  • Figure 5 is a cross-sectional view of the container 100 of Figure 4 from A-A
  • Figure 6 It is sectional drawing seen the container 100 of 4 from B-B.
  • FIG. 7 is a perspective view showing the structure of the partition wall 200 of the centrifuge Paul 1 according to an embodiment of the present invention
  • Figure 8 is a plan view of the partition wall 200
  • Figure 9 is a K of FIG. 10 is an enlarged view
  • FIG. 10 is a perspective view illustrating another form of the partition wall 200 of the Paul 1 for the centrifuge according to the exemplary embodiment of the present invention.
  • the centrifuge Paul (1) according to the present invention, the centrifuge Paul (1) for separating the substance in the tissue by using a centrifugal force, having a receiving portion 110 is accommodated tissue is rotated by the application of rotational force Container 100; Is disposed in the receiving portion 110 and the receiving portion 110 is divided into a radially inner space 120 and the outer space 130, but spaced apart from the bottom surface of the container 100 by a predetermined distance to the inside A partition wall 200 in which a gap capable of flowing between the space 120 and the outer space 130 is formed; And a pipe part 300 disposed in the inner space 120 and configured to access the tissue.
  • the bottom surface of the container 100 is formed with a recess 140 is the tissue is stacked, the lower portion of the partition wall 200 is a plurality of partitioning of the tissue flowing through the gap is made Cut portions 220 are provided, and the plurality of cut portions 220 are disposed to be spaced apart at predetermined intervals along the circumferential direction of the partition wall 200.
  • the container 100 is composed of a container having a receiving unit 110 configured as a predetermined accommodation space, and is configured to be rotatable according to the application of the rotational force.
  • the container 100 may be configured in a cylindrical shape for easy rotation.
  • the container 100 may be provided with a predetermined connection means for applying the rotational force, or may be provided with a predetermined member that can be connected to the connection means.
  • the receptacle 110 may accommodate a predetermined tissue for centrifugation, and may be filled with a material such as water and a predetermined cell tissue such as fat.
  • a predetermined cover 170 may be disposed on the upper portion of the container 100 to cover and seal the receiving portion 110 formed in the container 100, and the cover 170 may be connected for rotation application. Means may be provided, but are not limited to such.
  • the bottom portion of the container 100 may be formed with a depression 140 in which the tissue injected through the pipe portion 300 to be described later is deposited.
  • Recess 140 is a portion configured to be deeply recessed downward compared to the bottom surface of the container 100, may be configured to accumulate the tissue separated by centrifugation. A detailed structure is mentioned later.
  • the partition wall 200 is disposed in the accommodation unit 110.
  • the partition 200 includes a cylindrical body 210 as shown in FIG. 7, and the receiving part 110 is partitioned 200 as the partition 200 is disposed in the receiving part 110. It may be divided into an inner space 120 that is located inside and formed inside the partition in the radial direction and an outer space 130 that is formed outside the partition 200 in the radial direction.
  • the center of the partition wall 200 overlaps the center of the container 100 such that the partition wall 200 is located at the center of the receiving unit 110 in the container 100 so as to provide a centrifuge Paul 1. Easy rotation of can be achieved.
  • a gap having a predetermined interval H may be formed between the lower portion of the partition wall 200 and the bottom surface of the container 100 to allow tissue to move according to centrifugal separation. That is, the lower portion of the partition wall 200 may be configured to be spaced apart from the bottom surface of the container 100 by a predetermined distance to allow the flow of the tissue through the gap.
  • the lower portion of the partition 200 is provided with a cutting portion 220 to be cut and divided of the tissue. Specific configurations and effects of the cutting unit 220 will be described later.
  • the tube part 300 is disposed in the inner space 120.
  • the pipe part 300 is composed of a predetermined pipe extending in the vertical direction, the entrance and exit of tissue and water through the pipe part 300 can be made.
  • the centrifuge Paul (1) according to the present invention as laid down in the lying state, the extension direction of the pipe portion 300 can be seen in the left and right directions, the orientation of the centrifuge Paul (1) Therefore, the extension direction of the pipe portion 300 should be properly interpreted.
  • the pipe part 300 may be configured such that a lower end thereof extends into the recess 140, so that smooth injection and extraction of the tissue may be performed.
  • the upper portion of the pipe portion 300 is the entrance portion 400 is the injection and outflow of the tissue is disposed, the entry portion 400, the injection pipe 410 is configured to be symmetrical to each other in both sides direction And an extraction tube 420 and a wall portion (not shown) provided between the injection tube 410 and the extraction tube 420.
  • the entrance part 400 has an injection tube 410 and the extraction tube 420 are configured to be symmetrical with each other, the injection and extraction of the tissue can be easily made selectively without taking a separate configuration or connection method have.
  • the wall portion (not shown) is formed between the injection tube 410 and the extraction tube 420, it is possible to prevent the tissue from leaving the other tube during the injection and extraction of the tissue.
  • the lower portion of the access unit 400 may be provided with a predetermined seal member that can achieve a connection with the pipe portion 300 and achieve the airtight inside the receiving portion 110, but is not limited thereto.
  • the tissue is injected through the pipe part 300, when the container 100 is rotated, the tissue is pushed outward under centrifugal force.
  • the tissue and various substances contained in the container 100 may escape through the gap. It is driven to the outer space 130 divided through the partition wall 200.
  • the tissue and various materials, etc. are driven to the upper portion of the outer space 130 according to the rotation and are separated from each other according to various characteristic elements such as weight and the like.
  • the tissue driven into the outer space 130 falls and accumulates in the recessed portion 140 of the bottom surface of the vessel 100.
  • the tissues having the first stacked can be separated from the various substances in the tissue.
  • the lower portion of the partition wall 200 is provided with a plurality of cutting parts 220 to be divided into the tissue moving through the gap.
  • the plurality of cutouts 220 may have a configuration in which the plurality of cutouts 220 are spaced apart at predetermined intervals along the circumferential direction of the partition wall 200.
  • the tissue moves between the inner space 120 and the outer space 130 through the gap, and thus passes through the plurality of cutouts 220 provided in the lower portion of the partition wall 200. do.
  • cutting and dividing of the tissue may be performed. Accordingly, the tissue may be largely aggregated to prevent agglomeration and the tissue may be easily extracted through the pipe part 300.
  • collagen is separated from the adipose tissue and agglomerated into agglomerates in the container 100. May occur. Accordingly, the aggregated collagen may act as a large inhibitory factor in the operation of the centrifuge Paul by blocking the pipe part 300.
  • the centrifugal separator 1 has the cut portion 220 as described above, the aggregation phenomenon is prevented as described above, and thus the formation of such agglomeration can be prevented, thereby preventing the closing of the pipe part 300. have.
  • the tissue is divided and has a small volume, and the surface area is widened, when the separation and various reactions are to be performed by using a predetermined enzyme or the like, the enlarged surface area improves the reaction efficiency and shortens the reaction time. Separation can be achieved.
  • the centrifuge including the centrifuge Paul 1 according to the present invention may include a drive device such as a predetermined motor, a tissue supply device, etc., in addition to the centrifuge Paul 1.
  • each cut portion 220 is formed of a beam connected to the lower portion of the partition wall 200 and extends in the outward direction of the partition wall 200.
  • each cut portion 220 may have a configuration such as a predetermined bar or beam connected to the partition wall 200 and extending in the radially outward direction. Accordingly, the plurality of cutouts 220 may have a radial structure centered on the center of the partition wall 200, and the number and separation distance thereof are not limited. For example, as shown in FIG. 10, a plurality of cutting parts 220 disposed relatively densely may be provided, but is not limited thereto.
  • the plurality of cutouts 220 are at least a first cutout 220 and a second cutout 220 which is disposed symmetrically with respect to the first cutout 220 about the center of the container 100. ).
  • the partition wall 200 when the partition wall 200 is viewed from above, the plurality of cutouts 220 may be symmetrically formed about the center of the partition wall 200. Accordingly, for example, when any one cut portion 220 is referred to as the first cut portion M, the second cut portion N is symmetrically disposed about the center of the partition wall 200 with respect to the first cut portion M. FIG. ) May be present. Accordingly, the partition wall 200 disposed in the container 100 is stably supported and may be prevented from being shaken or excessively displaced in the centrifugal separation process.
  • the outer ends of the plurality of cut portions 220 are configured to be supported in contact with at least one of the inner surface and the bottom surface of the container 100.
  • the outer ends of the plurality of cut portions 220 are supported in contact with at least one of the inner surface and the bottom surface of the container 100, so that the partition wall 200 is supported in the container 100 so as to be appropriate.
  • Position fixation or position limitation may be made.
  • the cut portion 220 has a symmetrical arrangement and a configuration in which the cut portion 220 is in contact with and supported by at least one of the inner surface and the bottom surface, proper positioning or limiting of the partition wall 200 is achieved and in the centrifugal separation process.
  • the partition wall 200 may be prevented from shaking or being excessively displaced.
  • the cut portion 220 is configured to form a blade 230 for dividing the tissue on at least one side.
  • the cut portion 220 is relatively sharply polished to achieve the division of the tissue, accordingly the blade 230 is configured to have a sharp end to perform the cutting Can be. Accordingly, the division of the tissue passing through the cut portion 220 can be more easily achieved.
  • the blade 230 is illustrated to be formed at one side of the cut portion 220, but the blade 230 may be formed at any portion of the cut portion 220, and the cut portion 220 may be formed. May have any form.
  • At least a portion of the inner surface of the container 100, the centrifuge Paul (1) according to the invention in contact with the cutting portion 220 when rotating the partition 200 and the container 100 ) Is provided with a stopper 160 to rotate together, and the stopper 160 is configured to be disposed between at least two or more cutouts 220 of the plurality of cutouts 220.
  • the stopper 160 may be disposed on at least one of the spaces between the cut portions 220 on the inner surface of the container 100.
  • the stopper 160 is configured like a predetermined locking portion that contacts the cutout portion 220 so that the cutout portion 220 and the partition wall 200 no longer move their positions, and thus the container 100 and the partition wall ( 200 can rotate together. Accordingly, the cutting part 220 provided in the partition wall 200 may also rotate together and the cutting of the tissue flowing through the gap below the partition wall 200 may be performed.
  • the stopper 160 may be disposed on any one portion, but preferably, as shown in FIGS. 4 to 6, the stopper 160 may be symmetrically configured so as not to affect the rotation due to the centrifugation.
  • the partition wall 200 may be composed of a separate member independent of the container 100. Accordingly, the configuration of the centrifuge Paul 1 according to the present invention can be simplified.
  • the independent members may be understood to be composed of individual components, and in addition to the case where the components are spaced apart from each other, it should be understood to include the case of partial contact as described above.
  • the partition wall 200 and the container 100 may be configured by different independent members and may have an arrangement in which the partition wall 200 and the container 100 are partially contacted but not fastened or connected.
  • the partition wall 200 is configured to have a cylindrical shape extending in the vertical direction, and is configured to be tapered so that the lower width is larger than the upper width.
  • the partition wall 200 may have an inclination angle in the vertical direction, or may have a tapered configuration in which the thickness of each part is different in the vertical direction so that the lower width is larger than the upper width.
  • the width of the outer space 130 may have a lower width than an upper width. Accordingly, the tissue driven into the outer space 130 in the centrifugation process is more easily made to fall downward after the rotation is completed, the centrifugation efficiency can be increased.

Abstract

The present invention relates to a bowl for a centrifuge and the centrifuge including the same and, more specifically, to a bowl for a centrifuge and the centrifuge including the same, and the bowl for a centrifuge which is used for a bowl for a centrifuge for separating a material within a tissue by using centrifugal force, comprises: a container which has an accommodation portion having a predetermined tissue accommodated therein, and which is rotated along with the application of rotational force; a partition wall which is arranged inside the accommodation portion and partitions the accommodation portion into an inner space and an outer space in a diameter direction, wherein a gap is spaced from a bottom surface of the container at a predetermined distance such that the tissue is capable of moving between the inner space and the outer space; and a pipe portion arranged in the inner space so as to enable access of the tissue, wherein a recessed portion having the tissue stored therein is formed in the center of the bottom surface of the container, the partition wall is provided with a plurality of cutting portions for splitting the tissue moving through the gap, and the plurality of cutting portions are spaced at predetermined intervals in the circumferential direction of the partition wall.

Description

원심 분리기용 바울 및 그를 포함한 원심 분리기Paul for centrifuges and centrifuges including them
본 발명은 원심 분리기용 바울 및 그를 포함한 원심 분리기에 관한 것으로, 보다 상세하게는, 원심력을 이용하여 조직내 물질을 분리하는 원심 분리기에 사용되는 원심 분리기용 바울로서, 소정의 조직이 수용되는 수용부를 가지며 회전력의 인가에 따라 회전되는 용기; 상기 수용부 내에 배치되고 상기 수용부를 직경 방향 내공간과 외공간으로 구분하되 상기 용기의 바닥면과 소정 거리 이격되어 상기 조직이 상기 내공간과 상기 외공간 사이로 이동할 수 있는 간극이 형성된 격벽; 및 상기 내공간에 배치되며 조직의 출입이 이루어지는 관부; 를 포함하며,The present invention relates to a centrifuge Paul and a centrifuge including the same, and more particularly, a centrifuge Paul used in a centrifuge for separating a substance in a tissue by using a centrifugal force. It has a container that rotates in accordance with the application of the rotation force; A partition wall disposed in the accommodation part and partitioning the accommodation part into a radially inner space and an outer space, the partition having a gap spaced apart from the bottom surface of the container by a predetermined distance to move the tissue between the inner space and the outer space; And a pipe portion disposed in the inner space and configured to access the tissue. Including;
상기 용기의 바닥면 중심부에는 상기 조직이 적치되는 함몰부가 형성되며, 상기 격벽에는 상기 간극을 통해 이동하는 조직의 분할이 이루어지도록 하는 복수의 절단부가 구비되고, 상기 복수의 절단부는 상기 격벽의 둘레방향을 따라 소정의 간격을 갖고 이격되게 배치되는 원심 분리기용 바울 및 그를 포함한 원심 분리기에 관한 것이다.The bottom center of the container is provided with a depression in which the tissue is deposited, the partition is provided with a plurality of cuts for partitioning the tissue moving through the gap, the plurality of cuts are the circumferential direction of the partition The present invention relates to a centrifuge and a centrifuge including the same, which are arranged at a predetermined interval apart from each other.
지방 조직과 같은 소정의 조직으로부터 특정 세포, 또는 물질 등을 분리하기 위하여 원심 분리기가 사용되고 있다. 이러한 원심 분리기는 원심력을 사용하여 조직 내의 세포, 또는 물질 등을 분리하는 장치로서 그 구성 및 작동이 비교적 간단하고 용이한 분리를 제공하여 광범위하게 사용되어 왔다. Centrifuges are used to separate specific cells, substances, and the like from certain tissues, such as adipose tissue. The centrifuge has been widely used as a device for separating cells, substances, and the like in a tissue using centrifugal force, providing a relatively simple and easy separation thereof.
상기와 같은 원심 분리기에는 소정의 조직을 수용하고 회전가능하게 구성되어 원심 분리를 수행할 수 있는 소정의 바울(bowl)이 사용된다. 이러한 바울은 통상 회전이 용이하도록 원통형으로 구성됨이 보통이다.In the centrifuge as described above, a predetermined bowl is used to receive a predetermined tissue and to be rotatable to perform centrifugation. Such Paul is usually constructed in a cylindrical shape to facilitate rotation.
원심 분리를 위해 바울을 회전시키고, 원심 분리 후 추출된 조직 내의 각종 물질을 분리하여 원심 분리기용 바울로부터 추출할 때, 원심 분리 과정에서 분리된 각종 물질이 덩어리로 뭉쳐 추출이 어려워지는 경우가 종종 발생한다.When rotating the Paul for centrifugation, separating various substances in the extracted tissue after centrifugation, and extracting them from the centrifuge Paul, it is often difficult to extract the various substances separated into agglomerates during centrifugation. do.
일 예로, 지방 조직으로부터 세포를 추출하기 위하여, 지방 조직을 소정의 용기에 수용한 상태로 세척하거나 효소를 이용하여 지방 조직을 분리하는 경우, 지방 조직으로부터 콜라겐과 같은 소정의 물질이 분리되며, 이러한 물질이 뭉쳐서 용기 내에 잔류하여 분리된 물질의 추출 및 주입을 위한 출입관을 막아 장치의 손상 및 오작동을 일으키는 경우가 발생하는 경우가 있다.For example, in order to extract cells from the adipose tissue, when the adipose tissue is washed in a predetermined container or the adipose tissue is separated using an enzyme, a predetermined substance such as collagen is separated from the adipose tissue. In some cases, the material may clump together and remain in the container, blocking the entrance and exit of the separated material to cause damage and malfunction of the device.
따라서, 이러한 분리된 물질이 뭉치는 현상을 방지하여 원심 분리기의 작동 신뢰성을 확보하는 수단을 마련할 필요가 있다.Therefore, there is a need to provide a means for securing the operational reliability of the centrifuge by preventing agglomeration of these separated materials.
본 발명은 전술한 문제점을 해결하기 위해 안출된 것으로서, 소정의 조직이 수용되는 수용부를 가지며 회전력의 인가에 따라 회전되는 용기; 상기 수용부 내에 배치되고 상기 수용부를 직경 방향 내공간과 외공간으로 구분하되 상기 용기의 바닥면과 소정 거리 이격되어 상기 조직이 상기 내공간과 상기 외공간 사이로 이동할 수 있는 간극이 형성된 격벽; 및 상기 내공간에 배치되며 조직의 출입이 이루어지는 관부; 를 포함하며,The present invention has been made to solve the above-mentioned problems, the container having a receiving portion for receiving a predetermined tissue is rotated in accordance with the application of rotational force; A partition wall disposed in the accommodation part and partitioning the accommodation part into a radially inner space and an outer space, the partition having a gap spaced apart from the bottom surface of the container by a predetermined distance to move the tissue between the inner space and the outer space; And a pipe portion disposed in the inner space and configured to access the tissue. Including;
상기 용기의 바닥면 중심부에는 상기 조직이 적치되는 함몰부가 형성되며, 상기 격벽에는 상기 간극을 통해 이동하는 조직의 분할이 이루어지도록 하는 복수의 절단부가 구비되고, 상기 복수의 절단부는 상기 격벽의 둘레방향을 따라 소정의 간격을 갖고 이격되게 배치되는 원심 분리기용 바울을 제공하는 데 목적이 있다.The bottom center of the container is provided with a depression in which the tissue is deposited, the partition is provided with a plurality of cuts for partitioning the tissue moving through the gap, the plurality of cuts are the circumferential direction of the partition It is an object of the present invention to provide a Paul for centrifuge that is spaced apart at a predetermined interval along the.
상술한 목적을 달성하기 위하여, 본 발명에 따른 원심 분리기용 바울은, 원심력을 이용하여 조직내 물질을 분리하는 원심 분리기용 바울에 사용되는 원심 분리기용 바울로서, 소정의 조직이 수용되는 수용부를 가지며 회전력의 인가에 따라 회전되는 용기; 상기 수용부 내에 배치되고 상기 수용부를 직경 방향 내공간과 외공간으로 구분하되 상기 용기의 바닥면과 소정 거리 이격되어 상기 조직이 상기 내공간과 상기 외공간 사이로 이동할 수 있는 간극이 형성된 격벽; 및 상기 내공간에 배치되며 조직의 출입이 이루어지는 관부; 를 포함하며,In order to achieve the above object, the centrifuge Paul according to the present invention, the centrifuge Paul used in the centrifuge Paul for separating the material in the tissue by using the centrifugal force, has a receiving portion for receiving a predetermined tissue. A container rotated according to the application of the rotational force; A partition wall disposed in the accommodation part and partitioning the accommodation part into a radially inner space and an outer space, the partition having a gap spaced apart from the bottom surface of the container by a predetermined distance to move the tissue between the inner space and the outer space; And a pipe portion disposed in the inner space and configured to access the tissue. Including;
상기 용기의 바닥면 중심부에는 상기 조직이 적치되는 함몰부가 형성되며, 상기 격벽에는 상기 간극을 통해 이동하는 조직의 분할이 이루어지도록 하는 복수의 절단부가 구비되고, 상기 복수의 절단부는 상기 격벽의 둘레방향을 따라 소정의 간격을 갖고 이격되게 배치된다.The bottom center of the container is provided with a depression in which the tissue is deposited, the partition is provided with a plurality of cuts for partitioning the tissue moving through the gap, the plurality of cuts are the circumferential direction of the partition Are spaced apart along the predetermined interval.
바람직하게는, 상기 격벽은, 상기 용기와 별개의 부재로 독립되게 구성된다.Preferably, the partition wall is configured to be independent of the container.
바람직하게는, 상기 격벽은, 상하 방향으로 연장된 원통형으로 구성되되, 하측 단면적이 상측 단면적보다 크도록 테이퍼지게 구성된다.Preferably, the partition wall is formed in a cylindrical shape extending in the vertical direction, the lower cross-sectional area is configured to be tapered to be larger than the upper cross-sectional area.
바람직하게는, 상기 각각의 절단부는, 상기 격벽의 하부에 연결되며 상기 격벽의 외측 방향으로 연장되는 빔으로 구성된다.Preferably, each cut portion is composed of a beam connected to the lower portion of the partition wall and extending in the outward direction of the partition wall.
바람직하게는, 상기 복수의 절단부는, 적어도 제1 절단부, 및 상기 용기의 중심을 중심으로 상기 제1 절단부에 대해서 대칭으로 배치되는 제2 절단부를 포함한다.Preferably, the plurality of cutouts includes at least a first cutout and a second cutout that is symmetrically disposed with respect to the first cutout about the center of the container.
바람직하게는, 상기 복수의 절단부의 외측 단부는, 상기 용기의 내측면 및 바닥면 중 적어도 하나에 접촉하여 지지되게 구성된다.Preferably, the outer ends of the plurality of cut portions are configured to be supported in contact with at least one of the inner surface and the bottom surface of the container.
바람직하게는, 상기 절단부는, 적어도 일 측면에 상기 조직의 분할을 수행하는 블레이드가 형성되며, 상기 블레이드는 절단을 수행할 수 있도록 예리한 단부를 갖게 구성된다.Preferably, the cutting portion, the blade for performing the division of the tissue is formed on at least one side, the blade is configured to have a sharp end to perform the cutting.
바람직하게는, 상기 용기의 내측면의 적어도 일 부분에는, 회전시 상기 절단부에 접촉하여 상기 격벽과 상기 용기가 함께 회전하도록 하는 스토퍼가 구비되며, 상기 스토퍼는, 상기 복수의 절단부 중 적어도 둘 이상의 절단부 사이에 배치된다.Preferably, at least one portion of the inner surface of the container is provided with a stopper for contacting the cutting portion to rotate the partition and the container to rotate together, wherein the stopper, at least two or more cutting portions of the plurality of cutting portions Is placed in between.
바람직하게는, 상기 관부는, 하단부가 상기 함몰부 내부까지 연장되도록 배치되게 구성된다.Preferably, the pipe portion is configured such that the lower end portion is extended to the inside of the depression.
바람직하게는, 상기 관부의 상부에 배치되며 조직의 주입 및 유출이 이루어지는 출입부를 더 포함하며, 상기 출입부는, 양 측방향으로 서로 대칭되게 구성되는 주입관과 추출관, 및 상기 주입관과 추출관 사이에 구비되는 벽부를 포함하게 구성된다.Preferably, the apparatus further includes an entrance part disposed above the pipe part and configured to inject and outflow tissue, wherein the entrance part comprises an injection tube and an extraction tube configured to be symmetrical with each other in both lateral directions, and the injection tube and the extraction tube. It is comprised including the wall part provided in between.
본 발명에 따른 원심 분리기용 바울은, 용기 내의 수용부를 구분하는 격벽을 포함하며, 격벽의 하부에는 격벽과 용기 사이의 간극을 통해 이동하는 조직의 분할이 이루어지도록 하는 복수의 절단부가 구비되어 조직의 분할이 이루어질 수 있다.Paul for centrifugal separator according to the present invention includes a partition for dividing the receiving portion in the container, the lower portion of the partition is provided with a plurality of cutting parts to be divided into the tissue moving through the gap between the partition and the container of the tissue Partitioning can be made.
이에 따라서 조직이 크게 뭉쳐 덩어리를 이루는 것이 방지되고 상기 조직이 관부를 막는 것이 방지되어 관부를 통해 조직을 추출하는 것이 용이하게 이루어질 수 있다. Accordingly, it is possible to prevent the tissues from being largely aggregated to form agglomerates and to prevent the tissues from blocking the pipes, thereby easily extracting the tissues through the pipes.
뿐만 아니라, 상기 조직이 분할되어 작은 부피를 가져 표면적이 넓어짐에 따라서 소정의 효소 등을 사용하여 분리 및 각종 반응을 일으키고자 할 경우 넓어진 표면적으로 인해 반응 효율이 향상되고 반응 시간이 단축되어 더욱 유리한 원심 분리를 달성할 수 있다.In addition, as the tissue is divided and has a small volume, and the surface area is widened, when the separation and various reactions are to be performed by using a predetermined enzyme or the like, the enlarged surface area improves the reaction efficiency and shortens the reaction time. Separation can be achieved.
도 1 은 본 발명의 일 실시예에 따른 원심 분리기용 바울의 사시도이다. 1 is a perspective view of a centrifuge Paul according to an embodiment of the present invention.
도 2 는 본 발명에 따른 원심 분리기용 바울의 측면도이다.2 is a side view of a Paul for centrifuge according to the present invention.
도 3 은 본 발명의 일 실시예에 따른 원심 분리기용 바울의 구조를 나타낸 단면도이다.Figure 3 is a cross-sectional view showing the structure of the centrifuge Paul according to an embodiment of the present invention.
도 4 는 본 발명의 일 실시예에 따른 원심 분리기용 바울의 용기의 평면도이다.4 is a plan view of a vessel of Paul for centrifuge according to an embodiment of the present invention.
도 5 는 도 4 의 용기를 A - A 에서 바라본 단면도이다.5 is a cross-sectional view of the container of FIG. 4 as seen from A-A.
도 6 은 도 4 의 용기를 B - B 에서 바라본 단면도이다.FIG. 6 is a cross-sectional view of the container of FIG. 4 as seen from B-B. FIG.
도 7 은 본 발명의 일 실시예에 따른 원심 분리기용 바울의 격벽의 구조를 나타낸 사시도이다.Figure 7 is a perspective view showing the structure of the partition wall of the Paul for centrifuge according to an embodiment of the present invention.
도 8 은 격벽을 상방향에서 바라본 평면도이다.8 is a plan view of the partition wall viewed from above;
도 9 는 도 7 의 K 를 확대 도시한 도면이다. FIG. 9 is an enlarged view of K of FIG. 7.
도 10 은 본 발명의 일 실시예에 따른 원심 분리기용 바울의 다른 형태의 격벽을 나타낸 사시도이다.10 is a perspective view showing another form of partition wall of the centrifuge Paul according to an embodiment of the present invention.
본 발명은 원심 분리기용 바울 및 그를 포함한 원심 분리기에 관한 것으로, 보다 상세하게는, 본 발명에 따른 원심 분리기용 바울은, 원심력을 이용하여 조직내 물질을 분리하는 원심 분리기에 사용되는 원심 분리기용 바울로서, 소정의 조직이 수용되는 수용부를 가지며 회전력의 인가에 따라 회전되는 용기; 상기 수용부 내에 배치되고 상기 수용부를 직경 방향 내공간과 외공간으로 구분하되 상기 용기의 바닥면과 소정 거리 이격되어 상기 조직이 상기 내공간과 상기 외공간 사이로 이동할 수 있는 간극이 형성된 격벽; 및 상기 내공간에 배치되며 조직의 출입이 이루어지는 관부; 를 포함하며,The present invention relates to a centrifuge Paul and a centrifuge including the same, and more particularly, the centrifuge Paul according to the present invention, the centrifuge Paul used in the centrifuge for separating the material in the tissue using the centrifugal force. As a container having a receiving portion for receiving a predetermined tissue is rotated in accordance with the application of rotational force; A partition wall disposed in the accommodation part and partitioning the accommodation part into a radially inner space and an outer space, the partition having a gap spaced apart from the bottom surface of the container by a predetermined distance to move the tissue between the inner space and the outer space; And a pipe portion disposed in the inner space and configured to access the tissue. Including;
상기 용기의 바닥면 중심부에는 상기 조직이 적치되는 함몰부가 형성되며, 상기 격벽에는 상기 간극을 통해 이동하는 조직의 분할이 이루어지도록 하는 복수의 절단부가 구비되고, 상기 복수의 절단부는 상기 격벽의 둘레방향을 따라 소정의 간격을 갖고 이격되게 배치되게 구성된다.The bottom center of the container is provided with a depression in which the tissue is deposited, the partition is provided with a plurality of cuts for partitioning the tissue moving through the gap, the plurality of cuts are the circumferential direction of the partition It is configured to be spaced apart at a predetermined interval along the.
이하, 첨부된 도면을 참조하여, 본 발명에 따른 바람직한 실시예에 대하여 설명한다. 본 실시예는 제한적인 것으로 의도된 것이 아니다.Hereinafter, exemplary embodiments of the present invention will be described with reference to the accompanying drawings. This embodiment is not intended to be limiting.
본 발명의 이점 및 특징, 그리고 그것들을 달성하는 방법은 첨부되는 도면과 함께 상세하게 후술되어 있는 실시예들을 참조하면 명확해질 것이다. 그러나 본 발명은 이하에서 개시되는 실시예들에 한정되는 것이 아니라 서로 다른 다양한 형태로 구현될 수 있으며, 단지 본 실시예들은 본 발명의 개시가 완전하도록 하고, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위해 제공되는 것이며, 본 발명은 청구항의 범주에 의해 정의될 뿐이다. 명세서 전체에 걸쳐 동일 참조 부호는 동일 구성 요소를 지칭한다.Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but can be implemented in various different forms, and only the embodiments make the disclosure of the present invention complete, and the general knowledge in the art to which the present invention belongs. It is provided to fully inform the person having the scope of the invention, which is defined only by the scope of the claims. Like reference numerals refer to like elements throughout.
공간적으로 상대적인 용어인 "아래(below)", "아래(beneath)", "하부(lower)", "위(above)", "상부(upper)" 등은 도면에 도시되어 있는 바와 같이 하나의 부재 또는 구성 요소들과 다른 소자 또는 구성 요소들과의 상관관계를 용이하게 기술하기 위해 사용될 수 있다. 공간적으로 상대적인 용어는 도면에 도시되어 있는 방향에 더하여 사용시 또는 동작 시 부재의 서로 다른 방향을 포함하는 용어로 이해되어야 한다. 예를 들면, 도면에 도시되어 있는 부재를 뒤집을 경우 "상부" 는 "하부"로 해석될 수 있다. 부재는 다른 방향으로도 배향될 수 있고, 이에 따라 공간적으로 상대적인 용어들은 배향에 따라 해석될 수 있다.The spatially relative terms " below ", " beneath ", " lower ", " above ", " upper " It can be used to easily describe the correlation of a member or component with other elements or components. Spatially relative terms are to be understood as including terms in different directions of the member in use or operation in addition to the directions shown in the figures. For example, when the member shown in the figure is reversed, "upper" may be interpreted as "lower". The member can also be oriented in other directions, so that spatially relative terms can be interpreted according to the orientation.
본 명세서에서 사용된 용어는 실시예들을 설명하기 위한 것이며 본 발명을 제한하고자 하는 것은 아니다. 본 명세서에서, 단수형은 문구에서 특별히 언급하지않는 한 복수형도 포함한다. 명세서에서 사용되는 "포함한다(comprises)" 및/또는 "포함하는(comprising)"은 언급된 구성요소, 단계, 동작 및/또는 부재는 하나 이상의 다른 구성요소, 단계, 동작 및/또는 부재의 존재 또는 추가를 배제하지않는다.The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In this specification, the singular forms also include the plural unless specifically stated otherwise in the text. As used herein, "comprises" and / or "comprising" refers to the presence of one or more other components, steps, actions and / or members. Or does not exclude additions.
다른 정의가 없다면, 본 명세서에서 사용되는 모든 용어(기술 및 과학적 용어를 포함)는 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 공통적으로 이해될 수 있는 의미로 사용될 수 있을 것이다. 또 일반적으로 사용되는 사전에 정의되어 있는 용어들은 명백하게 특별히 정의되어있지 않은 한 이상적으로 또는 과도하게 해석되지 않는다.Unless otherwise defined, all terms (including technical and scientific terms) used in the present specification may be used in a sense that can be commonly understood by those skilled in the art. In addition, the terms defined in the commonly used dictionaries are not ideally or excessively interpreted unless they are specifically defined clearly.
도 1 은 본 발명의 일 실시예에 따른 원심 분리기용 바울(1)의 사시도이며, 도 2 는 본 발명에 따른 원심 분리기용 바울(1)의 측면도이고, 도 3 은 본 발명의 일 실시예에 따른 원심 분리기용 바울(1)의 구조를 나타낸 단면도이다.1 is a perspective view of a centrifuge Paul (1) according to an embodiment of the present invention, Figure 2 is a side view of the centrifuge Paul (1) according to the present invention, Figure 3 is an embodiment of the present invention Sectional drawing which shows the structure of the centrifuge Paul (1).
도 4 는 본 발명의 일 실시예에 따른 원심 분리기용 바울(1)의 용기(100)의 평면도이고, 도 5 는 도 4 의 용기(100)를 A - A 에서 바라본 단면도이며, 도 6 은 도 4 의 용기(100)를 B - B 에서 바라본 단면도이다.Figure 4 is a plan view of the container 100 of the centrifuge Paul 1 according to an embodiment of the present invention, Figure 5 is a cross-sectional view of the container 100 of Figure 4 from A-A, Figure 6 It is sectional drawing seen the container 100 of 4 from B-B.
도 7 은 본 발명의 일 실시예에 따른 원심 분리기용 바울(1)의 격벽(200)의 구조를 나타낸 사시도이며, 도 8 은 상기 격벽(200)의 평면도이며, 도 9 는 도 7 의 K 를 확대 도시한 도면이고, 도 10 은 본 발명의 일 실시예에 따른 원심 분리기용 바울(1)의 다른 형태의 격벽(200)을 나타낸 사시도이다.7 is a perspective view showing the structure of the partition wall 200 of the centrifuge Paul 1 according to an embodiment of the present invention, Figure 8 is a plan view of the partition wall 200, Figure 9 is a K of FIG. 10 is an enlarged view, and FIG. 10 is a perspective view illustrating another form of the partition wall 200 of the Paul 1 for the centrifuge according to the exemplary embodiment of the present invention.
본 발명에 따른 원심 분리기용 바울(1)은, 원심력을 이용하여 조직내 물질을 분리하는 원심 분리기용 바울(1)로서, 조직이 수용되는 수용부(110)를 가지며 회전력의 인가에 따라 회전되는 용기(100); 상기 수용부(110) 내에 배치되며 상기 수용부(110)를 직경 방향 내공간(120)과 외공간(130)으로 구분하되 상기 용기(100)의 바닥면과 소정 거리 이격되어 상기 조직이 상기 내공간(120)과 외공간(130) 사이를 유동할 수 있는 간극이 형성된 격벽(200); 및 상기 내공간(120)에 배치되며 조직의 출입이 이루어지는 관부(300); 를 포함하며, 상기 용기(100)의 바닥면 중심부에는 상기 조직이 적치되는 함몰부(140)가 형성되고, 상기 격벽(200)의 하부에는 상기 간극을 통해 유동하는 조직의 분할이 이루어지도록 하는 복수의 절단부(220)가 구비되며, 상기 복수의 절단부(220)는 상기 격벽(200)의 둘레방향을 따라 소정의 간격을 갖고 이격되게 배치된다.The centrifuge Paul (1) according to the present invention, the centrifuge Paul (1) for separating the substance in the tissue by using a centrifugal force, having a receiving portion 110 is accommodated tissue is rotated by the application of rotational force Container 100; Is disposed in the receiving portion 110 and the receiving portion 110 is divided into a radially inner space 120 and the outer space 130, but spaced apart from the bottom surface of the container 100 by a predetermined distance to the inside A partition wall 200 in which a gap capable of flowing between the space 120 and the outer space 130 is formed; And a pipe part 300 disposed in the inner space 120 and configured to access the tissue. It includes, the bottom surface of the container 100 is formed with a recess 140 is the tissue is stacked, the lower portion of the partition wall 200 is a plurality of partitioning of the tissue flowing through the gap is made Cut portions 220 are provided, and the plurality of cut portions 220 are disposed to be spaced apart at predetermined intervals along the circumferential direction of the partition wall 200.
용기(100)는 소정의 수용 공간으로 구성된 수용부(110)를 갖는 통으로 구성되며, 회전력의 인가에 따라 회전가능하게 구성된다. 바람직하게는, 용이한 회전을 위해 상기 용기(100)는 원통형으로 구성될 수 있다. 한편, 상기 용기(100)에는 회전력의 인가를 위해 소정의 연결 수단이 구비되거나, 또는 연결 수단과 연결될 수 있는 소정의 부재가 구비될 수 있다. 상기 수용부(110)에는 원심분리를 위한 소정의 조직이 수용될 수 있으며, 예컨대 지방과 같은 소정의 세포 조직 및 물과 같은 물질이 충진될 수 있다. 한편, 상기 용기(100)의 상부에는 용기(100)에 형성된 수용부(110)를 덮어 밀봉하는 소정의 덮개(170)가 배치될 수 있으며, 상기 덮개(170)에 상술한 회전 인가를 위한 연결 수단이 마련될 수 있고, 이에 한정하지 아니한다.The container 100 is composed of a container having a receiving unit 110 configured as a predetermined accommodation space, and is configured to be rotatable according to the application of the rotational force. Preferably, the container 100 may be configured in a cylindrical shape for easy rotation. On the other hand, the container 100 may be provided with a predetermined connection means for applying the rotational force, or may be provided with a predetermined member that can be connected to the connection means. The receptacle 110 may accommodate a predetermined tissue for centrifugation, and may be filled with a material such as water and a predetermined cell tissue such as fat. On the other hand, a predetermined cover 170 may be disposed on the upper portion of the container 100 to cover and seal the receiving portion 110 formed in the container 100, and the cover 170 may be connected for rotation application. Means may be provided, but are not limited to such.
바람직하게는, 상기 용기(100)의 바닥면 중심부에는 후술하는 관부(300)를 통해 주입된 조직이 적치되는 함몰부(140)가 형성될 수 있다. 함몰부(140)는 상기 용기(100)의 바닥면에 비해 하방으로 깊게 함몰되게 구성된 부분으로서, 원심 분리에 따라서 분리된 조직이 쌓이도록 구성될 수 있다. 구체적인 구성에 대해서는 후술한다.Preferably, the bottom portion of the container 100 may be formed with a depression 140 in which the tissue injected through the pipe portion 300 to be described later is deposited. Recess 140 is a portion configured to be deeply recessed downward compared to the bottom surface of the container 100, may be configured to accumulate the tissue separated by centrifugation. A detailed structure is mentioned later.
격벽(200)은 상기 수용부(110)에 배치된다. 상기 격벽(200)은 도 7 에 도시된 바와 같이 원통형 몸체(210)를 포함하여 구성되며, 상기 격벽(200)이 수용부(110) 내에 배치됨에 따라서 상기 수용부(110)는 격벽(200) 내에 위치하여 직경 방향으로 내측에 형성되는 내공간(120)과 격벽(200) 외부에 위치하여 직경 방향 외측에 형성되는 외공간(130)으로 구분될 수 있다. 바람직하게는, 상기 격벽(200)의 중심은 상기 용기(100)의 중심과 중첩되어 상기 격벽(200)이 용기(100) 내의 수용부(110)의 중앙에 위치하여 원심 분리기용 바울(1)의 용이한 회전이 달성될 수 있다.The partition wall 200 is disposed in the accommodation unit 110. The partition 200 includes a cylindrical body 210 as shown in FIG. 7, and the receiving part 110 is partitioned 200 as the partition 200 is disposed in the receiving part 110. It may be divided into an inner space 120 that is located inside and formed inside the partition in the radial direction and an outer space 130 that is formed outside the partition 200 in the radial direction. Preferably, the center of the partition wall 200 overlaps the center of the container 100 such that the partition wall 200 is located at the center of the receiving unit 110 in the container 100 so as to provide a centrifuge Paul 1. Easy rotation of can be achieved.
상기 격벽(200)의 하부와 상기 용기(100)의 바닥면 사이에는 원심 분리에 따라서 조직이 이동할 수 있도록 소정의 간격 H 을 갖는 간극이 형성될 수 있다. 즉, 상기 격벽(200)의 하부는 상기 용기(100)의 바닥면과 소정 거리 이격되게 구성되어 상기 간극을 통한 조직의 유동이 이루어지도록 할 수 있다.A gap having a predetermined interval H may be formed between the lower portion of the partition wall 200 and the bottom surface of the container 100 to allow tissue to move according to centrifugal separation. That is, the lower portion of the partition wall 200 may be configured to be spaced apart from the bottom surface of the container 100 by a predetermined distance to allow the flow of the tissue through the gap.
상기 격벽(200)의 하부에는 상기 조직의 절단 및 분할이 이루어지도록 하는 절단부(220)가 구비된다. 상기 절단부(220)의 구체적인 구성 및 효과에 대해서는 후술하기로 한다.The lower portion of the partition 200 is provided with a cutting portion 220 to be cut and divided of the tissue. Specific configurations and effects of the cutting unit 220 will be described later.
상기 내공간(120) 내에는 관부(300)가 배치된다. 상기 관부(300)는 상하 방향으로 연장된 소정의 관으로 구성되며, 상기 관부(300)를 통해 조직 및 물 등의 출입이 이루어질 수 있다. 한편, 상기 전제한 바와 같이 본 발명에 따른 원심 분리기용 바울(1)을 눕힌 상태로 둘 경우 상기 관부(300)의 연장 방향은 좌우 방향으로 볼 수 있으며, 원심 분리기용 바울(1)의 배향에 따라서 상기 관부(300)의 연장 방향은 적절하게 해석되어야 한다. 바람직하게는, 도 3 에 도시된 바와 같이, 상기 관부(300)는 하단부가 상기 함몰부(140) 내부까지 연장되도록 배치되게 구성되어 상기 조직의 원활한 주입 및 추출이 이루어지도록 할 수 있다.The tube part 300 is disposed in the inner space 120. The pipe part 300 is composed of a predetermined pipe extending in the vertical direction, the entrance and exit of tissue and water through the pipe part 300 can be made. On the other hand, when the centrifuge Paul (1) according to the present invention as laid down in the lying state, the extension direction of the pipe portion 300 can be seen in the left and right directions, the orientation of the centrifuge Paul (1) Therefore, the extension direction of the pipe portion 300 should be properly interpreted. Preferably, as shown in FIG. 3, the pipe part 300 may be configured such that a lower end thereof extends into the recess 140, so that smooth injection and extraction of the tissue may be performed.
바람직하게는, 상기 관부(300)의 상부에는 조직의 주입 및 유출이 이루어지는 출입부(400)가 배치되며, 상기 출입부(400)는, 양 측방향으로 서로 대칭되게 구성되는 주입관(410)과 추출관(420), 및 상기 주입관(410)과 추출관(420) 사이에 구비되는 벽부(미도시)를 포함하게 구성된다. Preferably, the upper portion of the pipe portion 300 is the entrance portion 400 is the injection and outflow of the tissue is disposed, the entry portion 400, the injection pipe 410 is configured to be symmetrical to each other in both sides direction And an extraction tube 420 and a wall portion (not shown) provided between the injection tube 410 and the extraction tube 420.
상기 출입부(400)는 서로 대칭되게 구성되는 주입관(410)과 추출관(420)을 구비함에 따라서, 별도의 구성 또는 연결 방식을 취함이 없이 조직의 주입 및 추출이 선택적으로 용이하게 이루어질 수 있다. 아울러, 상기 주입관(410)과 추출관(420) 사이에는 벽부(미도시)가 형성됨에 따라서 상기 조직의 주입 및 추출 과정에서 조직이 이탈하여 타 관으로 빠져나가는 것이 방지될 수 있다.The entrance part 400 has an injection tube 410 and the extraction tube 420 are configured to be symmetrical with each other, the injection and extraction of the tissue can be easily made selectively without taking a separate configuration or connection method have. In addition, as the wall portion (not shown) is formed between the injection tube 410 and the extraction tube 420, it is possible to prevent the tissue from leaving the other tube during the injection and extraction of the tissue.
한편, 상기 출입부(400)의 하부에는 상기 관부(300)와의 연결을 달성하고 수용부(110) 내부의 기밀을 달성할 수 있는 소정의 실 부재가 구비될 수 있으며, 이에 한정하지 아니한다.On the other hand, the lower portion of the access unit 400 may be provided with a predetermined seal member that can achieve a connection with the pipe portion 300 and achieve the airtight inside the receiving portion 110, but is not limited thereto.
이하에서는, 본 발명의 원심 분리기용 바울(1)의 구체적인 구성 및 그에 따른 작동에 대해 기술한다.In the following, the specific configuration of the centrifuge Paul 1 of the present invention and its operation will be described.
상술한 바와 같이, 상기 관부(300)를 통해 조직이 주입된 후, 상기 용기(100)를 회전시키면 상기 조직은 원심력을 받아 외측 방향으로 밀리게 된다. 이때, 상술한 바와 같이 상기 용기(100)의 바닥면과 상기 격벽(200)의 하부는 서로 이격되어 간극이 형성되므로, 상기 용기(100) 내에 수용된 조직 및 각종 물질 등은 상기 간극을 통해 빠져나가게 되며, 상기 격벽(200)을 통해 구분된 외공간(130)으로 몰리게 된다. 이때, 상기 조직 및 각종 물질 등은 회전에 따라서 외공간(130)의 상부로 몰리게 되며 중량 등과 같은 각종 특질적 요소에 따라서 서로 분리되어 뭉치게 된다. 한편, 원심 분리가 끝나서 용기(100)의 회전이 정지하면 상기 외공간(130)으로 몰린 조직은 낙하하여 상기 용기(100)의 바닥면의 함몰부(140)에 쌓이게 되며, 이때 주로 큰 중량을 갖는 조직이 먼저 쌓여 조직 내 각종 물질의 분리가 이루어질 수 있다.As described above, after the tissue is injected through the pipe part 300, when the container 100 is rotated, the tissue is pushed outward under centrifugal force. In this case, as described above, since the bottom surface of the container 100 and the lower portion of the partition wall 200 are spaced apart from each other to form a gap, the tissue and various substances contained in the container 100 may escape through the gap. It is driven to the outer space 130 divided through the partition wall 200. At this time, the tissue and various materials, etc. are driven to the upper portion of the outer space 130 according to the rotation and are separated from each other according to various characteristic elements such as weight and the like. On the other hand, when the rotation of the vessel 100 stops after the centrifugal separation, the tissue driven into the outer space 130 falls and accumulates in the recessed portion 140 of the bottom surface of the vessel 100. The tissues having the first stacked can be separated from the various substances in the tissue.
한편, 상술한 바와 같이, 상기 격벽(200)의 하부에는 상기 간극을 통해 이동하는 조직의 분할이 이루어지도록 하는 복수의 절단부(220)가 구비된다. 이때, 상기 복수의 절단부(220)는 상기 격벽(200)의 둘레방향을 따라 소정의 간격을 갖고 이격되게 배치되는 구성을 가질 수 있으며, 이에 따라서 조직의 분할이 이루어질 수 있다.On the other hand, as described above, the lower portion of the partition wall 200 is provided with a plurality of cutting parts 220 to be divided into the tissue moving through the gap. In this case, the plurality of cutouts 220 may have a configuration in which the plurality of cutouts 220 are spaced apart at predetermined intervals along the circumferential direction of the partition wall 200.
즉, 상기와 같이 원심 분리 과정에서 상기 조직은 상기 간극을 통해 내공간(120)과 외공간(130) 사이를 이동하므로, 상기 격벽(200)의 하부에 구비된 복수의 절단부(220)를 지나게 된다. 상기 절단부(220)를 통과함에 따라서 조직의 절단 및 분할이 이루어질 수 있으며, 이에 따라서 조직이 크게 뭉쳐 덩어리를 이루는 것이 방지되고 관부(300)를 통해 조직을 추출하는 것이 용이하게 이루어질 수 있다. That is, in the centrifugal separation process as described above, the tissue moves between the inner space 120 and the outer space 130 through the gap, and thus passes through the plurality of cutouts 220 provided in the lower portion of the partition wall 200. do. As the cutting part 220 passes, cutting and dividing of the tissue may be performed. Accordingly, the tissue may be largely aggregated to prevent agglomeration and the tissue may be easily extracted through the pipe part 300.
예컨대 지방 조직으로부터 세포를 추출하기 위하여 지방 조직을 용기(100)에 수용한 상태로 세척하거나 효소를 이용하여 분리할 경우, 지방 조직으로부터 콜라겐이 분리되어 용기(100) 내에서 덩어리로 뭉치는 현상이 발생할 수 있다. 이에 따라서, 이러한 덩어리로 뭉친 콜라겐은 상기 관부(300)를 막음으로써 원심 분리기용 바울의 작동에 큰 저해 요인으로 작용할 수 있다.For example, in order to extract cells from adipose tissue, when the adipose tissue is washed in a container 100 or separated using an enzyme, collagen is separated from the adipose tissue and agglomerated into agglomerates in the container 100. May occur. Accordingly, the aggregated collagen may act as a large inhibitory factor in the operation of the centrifuge Paul by blocking the pipe part 300.
그러나, 본 발명에 따른 원심 분리기용 바울(1)은 상기와 같은 절단부(220)를 가짐에 따라서 상기와 같이 뭉침 현상이 방지되고 따라서 이러한 덩어리 형성이 방지되어 관부(300)의 폐쇄가 방지될 수 있다.However, the centrifugal separator 1 according to the present invention has the cut portion 220 as described above, the aggregation phenomenon is prevented as described above, and thus the formation of such agglomeration can be prevented, thereby preventing the closing of the pipe part 300. have.
뿐만 아니라, 상기 조직이 분할되어 작은 부피를 가져 표면적이 넓어짐에 따라서 소정의 효소 등을 사용하여 분리 및 각종 반응을 일으키고자 할 경우 넓어진 표면적으로 인해 반응 효율이 향상되고 반응 시간이 단축되어 더욱 유리한 원심 분리를 달성할 수 있다.In addition, as the tissue is divided and has a small volume, and the surface area is widened, when the separation and various reactions are to be performed by using a predetermined enzyme or the like, the enlarged surface area improves the reaction efficiency and shortens the reaction time. Separation can be achieved.
아울러, 이러한 구성을 갖는 원심 분리기용 바울(1)을 사용한 원심 분리기의 경우, 상술한 바와 같이 원심 분리기용 바울(1)의 작동 효율이 증대됨에 따라서 원심 분리기 전체의 작동 효율 및 그에 따른 원심 분리 효율이 개선될 수 있다. 한편, 본 발명에 따른 원심 분리기용 바울(1)을 포함한 원심 분리기는 상기 원심 분리기용 바울(1) 외에 소정의 모터와 같은 구동 장치, 및 조직 공급 장치 등을 포함할 수 있다.In addition, in the case of the centrifuge using the centrifuge Paul (1) having such a configuration, as the operation efficiency of the centrifuge Paul (1) as described above is increased, the operating efficiency of the entire centrifuge and thus the centrifugal efficiency This can be improved. Meanwhile, the centrifuge including the centrifuge Paul 1 according to the present invention may include a drive device such as a predetermined motor, a tissue supply device, etc., in addition to the centrifuge Paul 1.
이하에서는 상기 절단부(220)의 구체적인 구성에 관해 설명한다.Hereinafter, a specific configuration of the cut part 220 will be described.
바람직하게는, 상기 각각의 절단부(220)는, 상기 격벽(200)의 하부에 연결되며 상기 격벽(200)의 외측 방향으로 연장되는 빔으로 구성된다.Preferably, each cut portion 220 is formed of a beam connected to the lower portion of the partition wall 200 and extends in the outward direction of the partition wall 200.
즉, 상기 각각의 절단부(220)는, 상기 격벽(200)에 연결되며 직경방향 외측 방향으로 연장되는 소정의 막대, 또는 빔과 같은 구성을 가질 수 있다. 이에 따라서, 복수의 절단부(220)는 상기 격벽(200)의 중심을 중심으로 하여 방사형 구조를 가질 수 있으며, 그 개수 및 이격 거리는 한정하지 아니한다. 일 예로, 도 10 에 도시된 바와 같이 비교적 빽빽하게 배치된 다수의 절단부(220)가 구비되는 것도 가능하며, 이에 한정하지 아니한다.That is, each cut portion 220 may have a configuration such as a predetermined bar or beam connected to the partition wall 200 and extending in the radially outward direction. Accordingly, the plurality of cutouts 220 may have a radial structure centered on the center of the partition wall 200, and the number and separation distance thereof are not limited. For example, as shown in FIG. 10, a plurality of cutting parts 220 disposed relatively densely may be provided, but is not limited thereto.
바람직하게는, 상기 복수의 절단부(220)는, 적어도 제1 절단부(220), 및 상기 용기(100)의 중심을 중심으로 상기 제1 절단부(220)에 대해서 대칭으로 배치되는 제2 절단부(220)를 포함한다.Preferably, the plurality of cutouts 220 are at least a first cutout 220 and a second cutout 220 which is disposed symmetrically with respect to the first cutout 220 about the center of the container 100. ).
즉, 예컨대 도 8 에 도시된 바와 같이, 상기 격벽(200)을 상부에서 볼 경우, 상기 복수의 절단부(220)는 상기 격벽(200)의 중심을 중심으로 하여 대칭으로 구성될 수 있다. 이에 따라서, 예컨대 어느 하나의 절단부(220)를 제1 절단부(M)라고 할 경우, 상기 제1 절단부(M)에 대해서 상기 격벽(200)의 중심을 중심으로 대칭되게 배치되는 제2 절단부(N)가 존재할 수 있다. 이에 따라서, 상기 용기(100) 내에 배치되는 상기 격벽(200)이 안정되게 지지되어 위치하며 원심 분리 과정에서 상기 격벽(200)이 흔들리거나 과도하게 변위하는 것이 방지될 수 있다.That is, for example, as shown in FIG. 8, when the partition wall 200 is viewed from above, the plurality of cutouts 220 may be symmetrically formed about the center of the partition wall 200. Accordingly, for example, when any one cut portion 220 is referred to as the first cut portion M, the second cut portion N is symmetrically disposed about the center of the partition wall 200 with respect to the first cut portion M. FIG. ) May be present. Accordingly, the partition wall 200 disposed in the container 100 is stably supported and may be prevented from being shaken or excessively displaced in the centrifugal separation process.
바람직하게는, 상기 복수의 절단부(220)의 외측 단부는, 상기 용기(100)의 내측면 및 바닥면 중 적어도 하나에 접촉하여 지지되게 구성된다.Preferably, the outer ends of the plurality of cut portions 220 are configured to be supported in contact with at least one of the inner surface and the bottom surface of the container 100.
즉, 상기 복수의 절단부(220)의 외측 단부는 상기 용기(100)의 내측면 및 바닥면 중 적어도 하나에 접촉하여 지지됨에 따라서, 상기 용기(100) 내에서 상기 격벽(200)이 지지되어 적절한 위치 고정 또는 위치 한정이 이루어지도록 할 수 있다. 상술한 바와 같이 절단부(220)가 대칭된 배치, 및 내측면 및 바닥면 중 적어도 하나에 접촉되어 지지되는 구성을 가짐에 따라서, 격벽(200)의 적절한 위치 고정 또는 한정이 달성되며 원심 분리 과정에서 상기 격벽(200)이 흔들리거나 과도하게 변위하는 것이 방지될 수 있다.That is, the outer ends of the plurality of cut portions 220 are supported in contact with at least one of the inner surface and the bottom surface of the container 100, so that the partition wall 200 is supported in the container 100 so as to be appropriate. Position fixation or position limitation may be made. As described above, as the cut portion 220 has a symmetrical arrangement and a configuration in which the cut portion 220 is in contact with and supported by at least one of the inner surface and the bottom surface, proper positioning or limiting of the partition wall 200 is achieved and in the centrifugal separation process. The partition wall 200 may be prevented from shaking or being excessively displaced.
바람직하게는, 상기 절단부(220)는, 적어도 일 측면에 상기 조직의 분할을 수행하는 블레이드(230)가 형성되게 구성된다.Preferably, the cut portion 220 is configured to form a blade 230 for dividing the tissue on at least one side.
즉, 도 9 에 도시된 바와 같이, 상기 절단부(220)는, 상기 조직의 분할을 달성할 수 있도록 비교적 날카롭게 연마되며, 이에 따라서 상기 블레이드(230)는 절단을 수행할 수 있도록 예리한 단부를 갖게 구성될 수 있다. 이에 따라서, 상기 절단부(220)를 통과하는 조직의 분할이 더욱 용이하게 달성될 수 있다. 한편, 도 9 에서는 상기 블레이드(230)가 상기 절단부(220)의 일 측에 형성되게 도시되었으나, 상기 블레이드(230)는 상기 절단부(220)의 임의의 부분에 형성될 수 있고, 절단부(220)는 임의의 형태를 가질 수 있다.That is, as shown in Figure 9, the cut portion 220 is relatively sharply polished to achieve the division of the tissue, accordingly the blade 230 is configured to have a sharp end to perform the cutting Can be. Accordingly, the division of the tissue passing through the cut portion 220 can be more easily achieved. Meanwhile, in FIG. 9, the blade 230 is illustrated to be formed at one side of the cut portion 220, but the blade 230 may be formed at any portion of the cut portion 220, and the cut portion 220 may be formed. May have any form.
바람직하게는, 상기 용기(100)의 내측면의 적어도 일 부분에는, 본 발명에 따른 원심 분리기용 바울(1)이 회전시 상기 절단부(220)에 접촉하여 상기 격벽(200)과 상기 용기(100)가 함께 회전하도록 하는 스토퍼(160)가 구비되며, 상기 스토퍼(160)는, 상기 복수의 절단부(220) 중 적어도 둘 이상의 절단부(220) 사이에 배치되게 구성된다.Preferably, at least a portion of the inner surface of the container 100, the centrifuge Paul (1) according to the invention in contact with the cutting portion 220 when rotating the partition 200 and the container 100 ) Is provided with a stopper 160 to rotate together, and the stopper 160 is configured to be disposed between at least two or more cutouts 220 of the plurality of cutouts 220.
즉, 상기 용기(100)의 내측면에는, 각각의 절단부(220) 사이 공간 중 적어도 하나에 상기 스토퍼(160)가 배치될 수 있다. 상기 스토퍼(160)는 상기 절단부(220)에 닿아 절단부(220) 및 격벽(200)이 더 이상 위치를 이동하지 못하도록 하는 소정의 걸림부와 같이 구성되어 이에 따라서 상기 용기(100)와 상기 격벽(200)이 함께 회전하도록 할 수 있다. 이에 따라서, 격벽(200)에 구비된 절단부(220)도 함께 회전하며 격벽(200) 하부의 간극을 통해 유동하는 조직의 절단이 이루어지도록 할 수 있다. 상기 스토퍼(160)는 어느 한 부분에 배치될 수 있으나, 바람직하게는 도 4 내지 도 6 에 도시된 바와 같이, 원심 분리에 따른 회전에 영향을 주지 않도록 대칭으로 구성될 수 있다.That is, the stopper 160 may be disposed on at least one of the spaces between the cut portions 220 on the inner surface of the container 100. The stopper 160 is configured like a predetermined locking portion that contacts the cutout portion 220 so that the cutout portion 220 and the partition wall 200 no longer move their positions, and thus the container 100 and the partition wall ( 200 can rotate together. Accordingly, the cutting part 220 provided in the partition wall 200 may also rotate together and the cutting of the tissue flowing through the gap below the partition wall 200 may be performed. The stopper 160 may be disposed on any one portion, but preferably, as shown in FIGS. 4 to 6, the stopper 160 may be symmetrically configured so as not to affect the rotation due to the centrifugation.
한편, 바람직하게는, 상기 격벽(200)은, 상기 용기(100)와 독립된 별개의 부재로 구성될 수 있다. 이에 따라서, 본 발명에 따른 원심 분리기용 바울(1)의 구성이 간단해질 수 있다. On the other hand, preferably, the partition wall 200, may be composed of a separate member independent of the container 100. Accordingly, the configuration of the centrifuge Paul 1 according to the present invention can be simplified.
여기서, 독립된 부재라 함은 각각 별개의 구성품으로 구성된다고 이해될 수 있으며, 서로 거리를 두고 이격되게 구성되는 경우 외에 상기와 같이 부분적으로 접촉하는 경우도 포함하는 것으로 이해되어야 한다. 일 예로, 상기 격벽(200)과 용기(100)가 서로 다른 독립된 부재로 구성되며 부분적으로 접촉하되 체결되거나 연결되지 않게 배치되는 배치를 가질 수 있는 것으로 이해될 수 있다.Herein, the independent members may be understood to be composed of individual components, and in addition to the case where the components are spaced apart from each other, it should be understood to include the case of partial contact as described above. For example, it may be understood that the partition wall 200 and the container 100 may be configured by different independent members and may have an arrangement in which the partition wall 200 and the container 100 are partially contacted but not fastened or connected.
또한, 바람직하게는, 상기 격벽(200)은, 상하 방향으로 연장된 원통형으로 구성되되, 하측 폭이 상측 폭보다 크도록 테이퍼지게 구성된다.Also, preferably, the partition wall 200 is configured to have a cylindrical shape extending in the vertical direction, and is configured to be tapered so that the lower width is larger than the upper width.
즉, 도 3 에 도시된 바와 같이, 상기 격벽(200)은 상하 방향으로 기울임 각도를 갖거나, 또는 상하 방향으로 각부의 두께가 상이하게 구성되어 하측 폭이 상측 폭보다 크도록 테이퍼진 구성을 가질 수 있다. 달리 설명하면, 상기 외공간(130)의 폭은 하측 폭이 상측 폭보다 작을 수 있다. 이에 따라서, 원심 분리 과정에서 외공간(130)으로 몰린 조직이 회전이 끝난 후 하방으로 낙하하는 것이 더욱 용이하게 이루어지며, 원심 분리 효율이 증대될 수 있다. That is, as shown in FIG. 3, the partition wall 200 may have an inclination angle in the vertical direction, or may have a tapered configuration in which the thickness of each part is different in the vertical direction so that the lower width is larger than the upper width. Can be. In other words, the width of the outer space 130 may have a lower width than an upper width. Accordingly, the tissue driven into the outer space 130 in the centrifugation process is more easily made to fall downward after the rotation is completed, the centrifugation efficiency can be increased.
이상에서는 바람직한 실시예에 대하여 도시하고 설명하였지만, 본 발명은 상술한 특정의 실시예에 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 발명이 속하는 기술분야에서 통상의 지식을 가진 자에 의해 다양한 변형실시가 가능한 것은 물론이고, 이러한 변형실시들은 본 발명의 기술적 사상이나 전망으로부터 개별적으로 이해되어서는 안될 것이다.Although the preferred embodiments have been illustrated and described above, the invention is not limited to the specific embodiments described above, and does not depart from the gist of the invention as claimed in the claims. Various modifications may be made by the person having the above, and these modifications should not be individually understood from the technical spirit or the prospect of the present invention.

Claims (11)

  1. 원심력을 이용하여 조직내 물질을 분리하는 원심 분리기용 바울에 있어서,In the centrifuge Paul for separating the material in the tissue using the centrifugal force,
    소정의 조직이 수용되는 수용부를 가지며 회전력의 인가에 따라 회전되는 용기;A container having an accommodation portion in which predetermined tissue is accommodated and rotated according to the application of rotational force;
    상기 수용부 내에 배치되고 상기 수용부를 직경 방향 내공간과 외공간으로 구분하되 상기 용기의 바닥면과 소정 거리 이격되어 상기 조직이 상기 내공간과 상기 외공간 사이로 이동할 수 있는 간극이 형성된 격벽;A partition wall disposed in the accommodation part and partitioning the accommodation part into a radially inner space and an outer space, the partition having a gap spaced apart from the bottom surface of the container by a predetermined distance to move the tissue between the inner space and the outer space;
    상기 내공간에 배치되며 조직의 출입이 이루어지는 관부; 를 포함하며,A pipe part disposed in the inner space and allowing access of the tissue; Including;
    상기 용기의 바닥면 중심부에는 상기 조직이 적치되는 함몰부가 형성되며, At the center of the bottom surface of the container is formed a depression in which the tissue is deposited,
    상기 격벽에는 상기 간극을 통해 이동하는 조직의 분할이 이루어지도록 하는 복수의 절단부가 구비되며,The partition wall is provided with a plurality of cuts to divide the tissue moving through the gap,
    상기 복수의 절단부는 상기 격벽의 둘레방향을 따라 소정의 간격을 갖고 이격되게 배치되는 원심 분리기용 바울.The plurality of cut portions Paul for centrifugal separator is arranged spaced apart at a predetermined interval along the circumferential direction of the partition wall.
  2. 제1항에 있어서,The method of claim 1,
    상기 격벽은,The partition wall,
    상기 용기와 별개의 부재로 독립되게 구성된 원심 분리기용 바울.Paul for centrifugal separators configured independently of the vessel.
  3. 제1항에 있어서,The method of claim 1,
    상기 격벽은,The partition wall,
    상하 방향으로 연장된 원통형으로 구성되되,Consists of a cylindrical extending in the vertical direction,
    하측 단면적이 상측 단면적보다 크도록 테이퍼지게 구성된 원심 분리기용 바울.Paul for centrifuges configured to taper so that the lower cross-sectional area is larger than the upper cross-sectional area.
  4. 제1항에 있어서,The method of claim 1,
    상기 각각의 절단부는,Each cut portion,
    상기 격벽의 하부에 연결되며 상기 격벽의 외측 방향으로 연장되는 빔으로 구성되는 원심 분리기용 바울.Paul for the centrifugal separator is connected to the bottom of the partition and composed of a beam extending in the outward direction of the partition.
  5. 제4항에 있어서,The method of claim 4, wherein
    상기 복수의 절단부는,The plurality of cutting parts,
    적어도 제1 절단부, 및 상기 용기의 중심을 중심으로 상기 제1 절단부에 대해서 대칭으로 배치되는 제2 절단부를 포함하는 원심 분리기용 바울.A Paul for a centrifuge comprising at least a first cutout and a second cutout disposed symmetrically with respect to the first cutout about a center of the container.
  6. 제4항에 있어서,The method of claim 4, wherein
    상기 복수의 절단부의 외측 단부는,The outer ends of the plurality of cutouts,
    상기 용기의 내측면 및 바닥면 중 적어도 하나에 접촉하여 지지되는 원심 분리기용 바울.Paul for centrifugal separator which is supported in contact with at least one of the inner and bottom surfaces of the container.
  7. 제4항에 있어서,The method of claim 4, wherein
    상기 절단부는,The cut portion,
    적어도 일 측면에 상기 조직의 분할을 수행하는 블레이드가 형성되며,On at least one side, a blade for partitioning the tissue is formed,
    상기 블레이드는 절단을 수행할 수 있도록 예리한 단부를 갖게 구성된 원심 분리기용 바울.The blade Paul for centrifuges having a sharp end to perform the cutting.
  8. 제4항에 있어서,The method of claim 4, wherein
    상기 용기의 내측면의 적어도 일 부분에는,At least a portion of the inner side of the container,
    회전시 상기 절단부에 접촉하여 상기 격벽과 상기 용기가 함께 회전하도록 하는 스토퍼가 구비되며,When the rotation is provided with a stopper for contacting the cutting portion to rotate the partition and the container together,
    상기 스토퍼는, 상기 복수의 절단부 중 적어도 둘 이상의 절단부 사이에 배치되는 원심 분리기용 바울.The stopper is a Paul for a centrifuge disposed between at least two or more cut out of the plurality of cut.
  9. 제1항에 있어서,The method of claim 1,
    상기 관부는,The tube part,
    하단부가 상기 함몰부 내부까지 연장되도록 배치되는 원심 분리기용 바울.Paul for the centrifuge is arranged so that the lower end extends into the depression.
  10. 제1항에 있어서,The method of claim 1,
    상기 관부의 상부에 배치되며 조직의 주입 및 추출이 이루어지는 출입부;를 더 포함하며,It is disposed on the upper portion of the pipe portion and the entrance portion through which the injection and extraction of tissue;
    상기 출입부는,The entrance portion,
    양 측방향으로 서로 대칭되게 구성되는 주입관과 추출관, 및An injection tube and an extraction tube configured to be symmetrical to each other in both lateral directions, and
    상기 주입관과 추출관 사이에 구비되는 벽부를 포함하는 원심 분리기용 바울.Paul for a centrifuge comprising a wall portion provided between the injection tube and the extraction tube.
  11. 제1항 내지 제10항 중 어느 한 항의 원심 분리기용 바울을 포함한 원심 분리기. A centrifugal separator comprising the centrifuge Paul of any one of claims 1 to 10.
PCT/KR2014/007879 2013-08-26 2014-08-25 Bowl for centrifuge and centrifuge including same WO2015030443A1 (en)

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WO2019245081A1 (en) * 2018-06-20 2019-12-26 주식회사 디오스템스 Centrifuge
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4692136A (en) * 1985-10-11 1987-09-08 Cardiovascular Systems Inc. Centrifuge
JPH07284529A (en) * 1994-01-21 1995-10-31 Haemonetics Corp Centrifuge bowl to process blood and its method
JPH09215946A (en) * 1996-02-13 1997-08-19 Hiroshi Kitada Screw type vertical centrifuge
JP2005095126A (en) * 2003-09-24 2005-04-14 Harumori Matayoshi Centrifugal separator

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4692136A (en) * 1985-10-11 1987-09-08 Cardiovascular Systems Inc. Centrifuge
JPH07284529A (en) * 1994-01-21 1995-10-31 Haemonetics Corp Centrifuge bowl to process blood and its method
JPH09215946A (en) * 1996-02-13 1997-08-19 Hiroshi Kitada Screw type vertical centrifuge
JP2005095126A (en) * 2003-09-24 2005-04-14 Harumori Matayoshi Centrifugal separator

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