WO2015013575A3 - Knockout mice derived from site specific recombinase - Google Patents
Knockout mice derived from site specific recombinase Download PDFInfo
- Publication number
- WO2015013575A3 WO2015013575A3 PCT/US2014/048122 US2014048122W WO2015013575A3 WO 2015013575 A3 WO2015013575 A3 WO 2015013575A3 US 2014048122 W US2014048122 W US 2014048122W WO 2015013575 A3 WO2015013575 A3 WO 2015013575A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- specific recombinase
- site specific
- knockout mice
- mice derived
- present disclosure
- Prior art date
Links
- 108010052160 Site-specific recombinase Proteins 0.000 title abstract 2
- 238000011813 knockout mouse model Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 2
- 102000018120 Recombinases Human genes 0.000 abstract 1
- 108010091086 Recombinases Proteins 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000001671 embryonic stem cell Anatomy 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 238000010172 mouse model Methods 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/20—Animal model comprising regulated expression system
- A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Husbandry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present disclosure provides methods for generating embryonic stem cell lines with the integration of a site-specific recombinase deriving from recombinase mouse models. Utilizing methods of the present disclosure, targeting vectors including selection cassettes flanked by respective recognition sites may be removed in ES cells and thus resulting progeny o chimeras would have absence of the selection cassette.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361858270P | 2013-07-25 | 2013-07-25 | |
US61/858,270 | 2013-07-25 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2015013575A2 WO2015013575A2 (en) | 2015-01-29 |
WO2015013575A3 true WO2015013575A3 (en) | 2015-04-02 |
Family
ID=52393982
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2014/048122 WO2015013575A2 (en) | 2013-07-25 | 2014-07-25 | Knockout mice derived from site specific recombinase |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2015013575A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017151668A1 (en) * | 2016-02-29 | 2017-09-08 | Wei Weng | Dividing of reporter proteins by dna sequences and its application in site specific recombination |
CN106577470B (en) * | 2016-10-19 | 2019-07-02 | 中国人民解放军第二军医大学 | A method of improving naked mole breeding potential |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6492575B1 (en) * | 1996-08-13 | 2002-12-10 | Boehringer Ingelheim International Gmbh | Method for developing transgenic mice |
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2014
- 2014-07-25 WO PCT/US2014/048122 patent/WO2015013575A2/en active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6492575B1 (en) * | 1996-08-13 | 2002-12-10 | Boehringer Ingelheim International Gmbh | Method for developing transgenic mice |
Non-Patent Citations (5)
Title |
---|
"Jax Mice and Services.", 11 July 2013 (2013-07-11), Retrieved from the Internet <URL:https://web.archive.org/web/20130711060017> [retrieved on 20141223] * |
BRANDA ET AL.: "Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice.", DEV. CELL, vol. 6, no. 1, January 2004 (2004-01-01), pages 7 - 28, XP002579368 * |
CHAN ET AL.: "KLF4 and PBX1 Directly Regulate NANOG Expression in Human Embryonic Stem Cells.", STEM CELL S, vol. 27, no. 9, September 2009 (2009-09-01), pages 2114 - 2125 * |
TAKEUCHI ET AL.: "Flp recombinase transgenic mice of C57BU6 strain for conditional gene targeting.", BIOCHEM BIOPHYS RES COMMUN, vol. 293, no. 3, 10 May 2002 (2002-05-10), pages 953 - 957, XP002365903, DOI: doi:10.1016/S0006-291X(02)00321-2 * |
XIAO ET AL.: "Newer Approaches to Genetic Modeling in Mice Tissue-Specific Protein Expression as Studied Using Angiotensin-Converting Enzyme (ACE).", AM J PATHOL, vol. 16, no. 3, September 2003 (2003-09-01), pages 807 - 817 * |
Also Published As
Publication number | Publication date |
---|---|
WO2015013575A2 (en) | 2015-01-29 |
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