WO2015013575A3 - Knockout mice derived from site specific recombinase - Google Patents

Knockout mice derived from site specific recombinase Download PDF

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Publication number
WO2015013575A3
WO2015013575A3 PCT/US2014/048122 US2014048122W WO2015013575A3 WO 2015013575 A3 WO2015013575 A3 WO 2015013575A3 US 2014048122 W US2014048122 W US 2014048122W WO 2015013575 A3 WO2015013575 A3 WO 2015013575A3
Authority
WO
WIPO (PCT)
Prior art keywords
specific recombinase
site specific
knockout mice
mice derived
present disclosure
Prior art date
Application number
PCT/US2014/048122
Other languages
French (fr)
Other versions
WO2015013575A2 (en
Inventor
Wei Weng
Yi Mei CHEN
Wei He
Original Assignee
Ingenious Targeting Laboratory, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingenious Targeting Laboratory, Inc. filed Critical Ingenious Targeting Laboratory, Inc.
Publication of WO2015013575A2 publication Critical patent/WO2015013575A2/en
Publication of WO2015013575A3 publication Critical patent/WO2015013575A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Husbandry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present disclosure provides methods for generating embryonic stem cell lines with the integration of a site-specific recombinase deriving from recombinase mouse models. Utilizing methods of the present disclosure, targeting vectors including selection cassettes flanked by respective recognition sites may be removed in ES cells and thus resulting progeny o chimeras would have absence of the selection cassette.
PCT/US2014/048122 2013-07-25 2014-07-25 Knockout mice derived from site specific recombinase WO2015013575A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361858270P 2013-07-25 2013-07-25
US61/858,270 2013-07-25

Publications (2)

Publication Number Publication Date
WO2015013575A2 WO2015013575A2 (en) 2015-01-29
WO2015013575A3 true WO2015013575A3 (en) 2015-04-02

Family

ID=52393982

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/048122 WO2015013575A2 (en) 2013-07-25 2014-07-25 Knockout mice derived from site specific recombinase

Country Status (1)

Country Link
WO (1) WO2015013575A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3423571A4 (en) * 2016-02-29 2019-10-30 Wei Weng Dividing of reporter proteins by dna sequences and its application in site specific recombination
CN106577470B (en) * 2016-10-19 2019-07-02 中国人民解放军第二军医大学 A method of improving naked mole breeding potential

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6492575B1 (en) * 1996-08-13 2002-12-10 Boehringer Ingelheim International Gmbh Method for developing transgenic mice

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6492575B1 (en) * 1996-08-13 2002-12-10 Boehringer Ingelheim International Gmbh Method for developing transgenic mice

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Jax Mice and Services.", 11 July 2013 (2013-07-11), Retrieved from the Internet <URL:https://web.archive.org/web/20130711060017> [retrieved on 20141223] *
BRANDA ET AL.: "Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice.", DEV. CELL, vol. 6, no. 1, January 2004 (2004-01-01), pages 7 - 28, XP002579368 *
CHAN ET AL.: "KLF4 and PBX1 Directly Regulate NANOG Expression in Human Embryonic Stem Cells.", STEM CELL S, vol. 27, no. 9, September 2009 (2009-09-01), pages 2114 - 2125 *
TAKEUCHI ET AL.: "Flp recombinase transgenic mice of C57BU6 strain for conditional gene targeting.", BIOCHEM BIOPHYS RES COMMUN, vol. 293, no. 3, 10 May 2002 (2002-05-10), pages 953 - 957, XP002365903, DOI: doi:10.1016/S0006-291X(02)00321-2 *
XIAO ET AL.: "Newer Approaches to Genetic Modeling in Mice Tissue-Specific Protein Expression as Studied Using Angiotensin-Converting Enzyme (ACE).", AM J PATHOL, vol. 16, no. 3, September 2003 (2003-09-01), pages 807 - 817 *

Also Published As

Publication number Publication date
WO2015013575A2 (en) 2015-01-29

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