WO2015008927A1 - Puce de biocapteur - Google Patents

Puce de biocapteur Download PDF

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Publication number
WO2015008927A1
WO2015008927A1 PCT/KR2014/004413 KR2014004413W WO2015008927A1 WO 2015008927 A1 WO2015008927 A1 WO 2015008927A1 KR 2014004413 W KR2014004413 W KR 2014004413W WO 2015008927 A1 WO2015008927 A1 WO 2015008927A1
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WO
WIPO (PCT)
Prior art keywords
sensor
blood
filter
flow path
biosensor chip
Prior art date
Application number
PCT/KR2014/004413
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English (en)
Korean (ko)
Inventor
함철호
나관구
박현구
김도균
Original Assignee
주식회사 미코
(주)미코바이오메드
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Publication of WO2015008927A1 publication Critical patent/WO2015008927A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells

Definitions

  • the present invention relates to a biosensor chip, and more particularly, to a biosensor chip for detecting a specific component from the blood after moving the blood using a capillary phenomenon.
  • Blood (whole blood) is composed of blood cell components such as red blood cells, white blood cells, and platelets, and plasma components such as water, proteins, fats, sugars, and other inorganic ions. Since the blood cell component, which is a tangible component, acts as an active ingredient that affects the analysis result, it is necessary to perform the test using only the plasma component so that the effect by the blood cell component can be excluded to obtain accurate results.
  • blood cell components such as red blood cells, white blood cells, and platelets
  • plasma components such as water, proteins, fats, sugars, and other inorganic ions. Since the blood cell component, which is a tangible component, acts as an active ingredient that affects the analysis result, it is necessary to perform the test using only the plasma component so that the effect by the blood cell component can be excluded to obtain accurate results.
  • Korean Patent Publication No. 2011-0045980 discloses a centrifugal separation method of blood using a centrifuge. In the case of using the centrifuge, it is possible to reliably separate the blood. However, it is difficult to apply to products for the general public because it requires a lot of time-consuming processes, requires a lot of time, and requires expertise and skill.
  • the present invention provides a biosensor chip that requires a small amount of blood, and can rapidly and accurately separate blood cells from the blood to perform analysis on plasma.
  • the biosensor chip according to the present invention includes a body, a blood injection hole provided at an upper surface of the body, and connected to the blood injection hole inside the body, and the blood injected through the blood injection hole is used to provide a capillary phenomenon.
  • the first filter is made of a porous material
  • the diameter of the hole may be 0.5 to 2 ⁇ m.
  • the first filter may include any one of cellulose, fusion 5, glass fiber, carbon fiber, cotton, and wool.
  • the second filter may be a plurality of microstructures having a columnar shape that crosses the flow path.
  • the spacing between the microstructures may be 0.5 to 1.5 ⁇ m.
  • the cross-sectional area of the portion provided with the second filter in the flow path may be larger than the cross-sectional area of the remaining portion.
  • the sensor may be any one of an electrochemical sensor, an electrical sensor and an optical sensor.
  • the sensor when the sensor is the electrochemical sensor, the sensor is provided to be connected to the sensor, at least a pair of electrodes for confirming the detection result of the sensor and the electrodes
  • the biosensor chip further comprises an opening that exposes to the outside.
  • the sensor when the sensor is the optical sensor, the sensor may further include an opening that exposes the sensor to confirm the response result of the sensor.
  • the sensor when the sensor is the electrical sensor, the sensor is provided to be connected to the sensor, at least a pair of electrodes for confirming the detection result of the sensor, and the electrodes It may further include an absorbent pad connected to the openings exposed to the outside and the end of the sensor, for absorbing the reaction residual liquid of the sensor.
  • a plurality of the flow path, the first filter, the second filter and the sensor may be provided in a radial manner with respect to the blood inlet.
  • the biosensor chip is provided to be connected to the flow path on the upper surface of the body, it may further include an air outlet for discharging the air in the flow path.
  • the body is composed of a lower substrate and an upper substrate provided on the lower substrate, the flow path, the second filter and the sensor is provided on the lower substrate, the blood An injection hole may be provided through the upper substrate, and the first filter may be provided between the upper substrate and the lower substrate.
  • the body is made of a lower substrate, an upper substrate provided on the lower substrate and a case provided to surround the lower substrate and the upper substrate, the flow path, the second filter And the sensor is provided on the lower substrate, the blood injection hole is provided through the upper substrate and the case, and the first filter may be provided between the upper substrate and the case.
  • the biosensor chip according to the present invention separates blood cells using a first filter and a second filter. Since the blood cells are filtered by the two filters, the blood cells can be accurately separated from the blood.
  • the flow path is connected from the blood inlet to the sensor, the blood may be delivered to the sensor without external power through the flow path according to the capillary phenomenon. Therefore, separation of the blood cells and detection of the sensor can be performed without the external power.
  • FIG. 1 is an exploded perspective view illustrating a biosensor chip according to an embodiment of the present invention.
  • FIG. 2 is a plan view illustrating the biosensor chip illustrated in FIG. 1.
  • FIG. 3 is a cross-sectional view illustrating the biosensor chip illustrated in FIG. 1.
  • FIG. 4 is a cross-sectional view for describing a biosensor chip according to another exemplary embodiment of the present invention.
  • 5 to 7 are plan views illustrating biosensor chips according to other embodiments of the present invention.
  • the biosensor chip is provided on the body, the upper surface of the body and the blood inlet for the blood is injected, and is provided to be connected to the blood inlet inside the body and injected through the blood inlet
  • a flow path for moving the collected blood through a capillary phenomenon, a first filter provided at the blood inlet to separate blood cells from the blood primarily, and a second filter provided at the flow path to secondly separate the blood cells from the blood It may include a filter and a sensor provided at the end of the flow path inside the body and reacts with the plasma separated from the blood cells in the blood and detects a specific component.
  • the first filter is made of a porous material
  • the diameter of the hole may be 0.5 to 2 ⁇ m.
  • the second filter may be a plurality of microstructures having a columnar shape that crosses the flow path.
  • the spacing between the microstructures may be 0.5 to 1.5 ⁇ m.
  • first and second may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another.
  • the first component may be referred to as the second component, and similarly, the second component may also be referred to as the first component.
  • FIG. 1 is an exploded perspective view illustrating a biosensor chip according to an exemplary embodiment of the present invention
  • FIG. 2 is a plan view illustrating the biosensor chip illustrated in FIG. 1
  • FIG. 3 is a biosensor illustrated in FIG. 1. It is sectional drawing for demonstrating a chip.
  • the biosensor chip 100 detects a specific component from the blood after moving the blood using a capillary phenomenon.
  • the body 110 includes an upper substrate 112 and a lower substrate 114 provided below the upper substrate 112.
  • the upper substrate 112 may have a blood inlet 120, a first air outlet 172, a second air outlet 174, and an opening 164.
  • the first filter 140 may be provided below the blood injection hole 120 of the upper substrate 112.
  • a flow path 130 and a second filter 150 may be formed on the upper surface of the lower substrate 114, and the sensor 160 and the electrode 162 may be provided.
  • Glass or synthetic resin may be used as the material of the upper substrate 112.
  • the synthetic resins include silicone, urethane, PVC, and mixtures thereof.
  • the material of the lower substrate 114 may be silicon.
  • the flow path 130 and the second filter 150, the sensor 160, and the electrode 162 using semiconductor manufacturing processes such as an etching process, an oxidation process, and a deposition process. ) To form a space for accommodating.
  • the upper substrate 112 and the lower substrate 114 may be coupled to each other by thermal bonding, compression bonding, or electrical bonding.
  • an intermediate layer may be provided between the upper substrate 112 and the lower substrate 114 to improve the bonding force between the upper substrate 112 and the lower substrate 114.
  • the intermediate layer may be made of an adhesive material.
  • Blood injection hole 120 is provided on the upper surface of the body (110). In detail, the blood injection hole 120 penetrates the upper and lower surfaces of the upper substrate 112. The blood is injected through the blood inlet 120.
  • the flow path 130 is provided to be connected to the blood injection hole 120 inside the body 110.
  • the flow path 130 extends along the horizontal direction inside the body 110. Specifically, the flow path 130 extends in the horizontal direction on the upper surface of the lower substrate 114.
  • the flow path 130 moves the blood injected through the blood inlet 120 to the sensor 160 through a capillary phenomenon.
  • the first filter 140 is provided in the blood inlet 120.
  • the first filter 140 is disposed between the upper substrate 112 and the lower substrate 114, and is disposed at a connection portion between the blood injection hole 120 and the flow path 130.
  • the first filter 140 has a size larger than the cross-sectional area of the blood inlet 120. Therefore, the first filter 140 may be stably fixed by the upper substrate 112 and the lower substrate 114.
  • the first filter 140 is primarily injected through the blood inlet 120 to separate the blood cells from the blood descending by gravity. At this time, the blood cells are approximately filtered by the first filter 140.
  • the first filter 140 is made of a porous material, the diameter of the hole may be equal to or smaller than the thickness of the blood cells.
  • the pore diameter of the first filter 140 may be about 0.5 to 2 ⁇ m.
  • the diameter of the hole exceeds about 2 ⁇ m, since the diameter of the hole is larger than the thickness of the blood cells, the effect of filtering the blood cells in the first filter 140 may be reduced.
  • the diameter of the hole is less than about 0.5 ⁇ m
  • the diameter of the hole is excessively smaller than the thickness of the blood cell, so that the blood cell may be accurately filtered through the first filter 140. Therefore, the first filter 140 may be blocked by the blood cells.
  • the diameter of the hole is less than about 0.5 ⁇ m, the hole is fine and it is difficult to manufacture the first filter 140 having a hole of the size.
  • Examples of the material of the first filter 140 include cellulose, fusion 5, glass fiber, carbon fiber, polymer fiber, cotton, wool, and the like. These may be used alone or in combination.
  • the second filter 150 is provided in the flow path 130.
  • the second filter 150 is provided in the flow path 130 formed on the upper surface of the lower substrate 114.
  • the second filter 150 may be a plurality of microstructures having a pillar shape that crosses the flow path 130.
  • the microstructures may be disposed to vertically traverse the inside of the flow path 130.
  • the microstructures may be disposed to traverse the inside of the flow path 130 from side to side.
  • the second filter 150 secondaryly separates the blood cells from the blood.
  • the blood cells are filtered by the second filter 150 while the blood cells are approximately filtered by the first filter 140. Therefore, the blood cells can be accurately separated from the blood by the first filter 140 and the second filter 150.
  • the microstructure has been described as having a columnar shape, the microstructure may have various shapes capable of filtering the blood cells, such as a mesh shape or a lattice shape.
  • the spacing between the microstructures may be slightly less than the thickness of the blood cells to more stably filter the blood cells.
  • the spacing between the microstructures may be about 0.5 to 1.5 ⁇ m.
  • the spacing between the microstructures exceeds about 1.5 ⁇ m, the spacing between the microstructures is similar to the thickness of the blood cells, so that the blood cells may partially contract and pass between the microstructures. Thus, microstructures may not correctly filter out the blood cells.
  • the spacing between the microstructures is less than about 0.5 ⁇ m, the spacing between the microstructures is excessively smaller than the thickness of the blood cells. Therefore, although the microstructures can precisely filter the blood cells, the blood cells may be blocked between the microstructures and the plasma of the blood may not pass through the microstructures.
  • the spacing between the microstructures is about 0.5 to 1.5 ⁇ m, when the cross-sectional area of the flow path 130 is small, the microstructures may be blocked by the blood cells. Therefore, the cross-sectional area of the portion where the second filter 150 is provided in the flow passage 130 may be larger than that of the remaining portions.
  • the width of the flow path 130 at the portion where the second filter 150 is provided may be larger than the width of the flow path 130 at the remaining portions.
  • the depth of the flow passage 130 at the portion where the second filter 150 is provided may be deeper than the depth of the flow passage 130 at the remaining portions.
  • the width of the flow path 130 at the portion where the second filter 150 is provided is greater than the width of the flow path 130 at the remaining portion, and the flow path 130 at the portion where the second filter 150 is provided. Depth of may also be deeper than the depth of the flow path 130 in the remaining portion.
  • the blood cells may block some of the microstructures, but block the entirety of the microstructures. You can prevent it. Thus, the blood plasma may pass through the microstructures of the second filter 150 to the sensor 160.
  • the flow rate of the blood may be increased in the portion where the second filter 150 is provided.
  • the blood can move quickly to the sensor 160.
  • the sensor 160 is provided at the end of the flow path 130 inside the body 110. Specifically, the sensor 160 is disposed at the end of the flow path 130 provided on the upper surface of the lower substrate 114. Blood injection ports 120 and the sensor 160 may be located at both ends of the flow path 130.
  • the blood injected into the blood inlet 120 passes through the flow path 130, and the blood cells are filtered by the first filter 140 and the second filter 150, and only the plasma reaches the sensor 160.
  • the sensor 160 reacts with the plasma, detects a specific component, and outputs a signal.
  • Sensor 160 may be an electrochemical sensor.
  • the sensor 160 includes an assay reagent, and the assay reagent causes a reaction such as a redox reaction with a specific substance in the plasma.
  • the sensor 160 may further include an electrode 162 and an opening 164.
  • the electrode 162 is formed in a pair and is provided to be connected to the sensor 160 inside the body 110. Specifically, the electrode 162 may be inserted into the upper surface of the lower substrate 114 to be connected to the sensor 160. One end of each of the pair of electrodes 162 is connected to the sensor 160.
  • the electrode 162 may be made of a metal material. Examples of the metal material 162 include gold and copper.
  • the opening 164 exposes the other end of the electrode 162 opposite to the one end to the outside.
  • the opening 164 is provided in the upper surface of the body 110, and specifically, passes through the upper and lower surfaces of the upper substrate 112.
  • the electrode 162 exposed by the opening 164 may be connected to the socket device of the meter when the biosensor chip 100 is inserted into the meter.
  • the sensor 160 detects a specific component in response to the plasma and the output signal is transmitted to the measuring instrument through the electrode 162.
  • a current signal is generated for a predetermined time while the reaction reagent of the sensor 160 reacts with a specific component of the plasma, and the biosensor chip ( In the state where 100) is inserted into the meter, the current signal may be read by the meter to quantitatively analyze specific components in the plasma.
  • the shape and structure of the electrode 162 included in the sensor 160 are not limited to the illustrated example, and various shapes and structures can be implemented, and a person skilled in the art can change the shape and structure of the electrode from the illustrated example. It may be changed appropriately.
  • the air outlet 170 is provided on the upper surface of the body 110. Specifically, the air outlet 170 penetrates up and down the upper substrate 112.
  • the air outlet 170 includes a first air outlet 172 and a second air outlet 174.
  • the first air outlet 172 is connected to a rear end of a portion of the flow path 130 provided with the second filter 150.
  • air in the flow path 130 at the portion where the second filter 150 is provided may be discharged to the outside through the first air outlet 172. Can be. Therefore, it is possible to prevent the generation of voids in the flow path 130 of the portion provided with the second filter 150.
  • the blood since the blood flow is not interrupted by the voids, the blood can move quickly to the sensor 160.
  • the second air outlet 174 is connected to a portion where the sensor 160 is provided.
  • the second air outlet 174 is connected to the other end of the sensor 160 opposite to one end of the sensor 160 connected to the flow path 130. Air at a portion where the sensor 160 is provided may be discharged to the outside through the second air outlet 174. Therefore, it is possible to prevent the generation of voids in the area provided with the sensor 160, the blood can be quickly moved to the entire sensor 160 without the void.
  • FIG. 4 is a cross-sectional view for describing a biosensor chip according to another exemplary embodiment of the present invention.
  • the biosensor chip 200 includes a body 210, a blood inlet 220, a flow path 230, a first filter 240, a second filter 250, a sensor 260, and an air outlet. 270.
  • the body 210 includes an upper substrate 212, a lower substrate 214 provided under the upper substrate 212, and a case 216 provided to surround the upper substrate 212 and the lower substrate 214. .
  • the blood inlet 220, the first air outlet 272, the second air outlet 274, and the opening 264 may pass through the upper substrate 212 and the case 216 on the upper substrate 212.
  • the material of the case 216 may be substantially the same as the material of the upper substrate 212.
  • glass or synthetic resin may be used as the material of the case 216.
  • the synthetic resins include silicone, urethane, PVC, and mixtures thereof.
  • the first filter 240 may be provided at the blood inlet 220 between the upper substrate 212 and the case 216.
  • the first filter 240 may be disposed between the upper substrate 212 and the lower substrate 214, and may be disposed at a connection portion between the blood injection hole 220 and the flow path 230.
  • biosensor chip 200 A detailed description of the biosensor chip 200 for the remaining parts except for the above description will be described with reference to FIGS. 1 to 3.
  • the case 216 may protect the upper substrate 212 and the lower substrate 214 from the impact. Therefore, the durability of the biosensor chip 200 can be improved.
  • 5 to 7 are plan views illustrating biosensor chips according to other embodiments of the present invention.
  • the biosensor chip 300 includes a body 310, a blood inlet 320, a flow path 330, a first filter 340, a second filter 350, a sensor 360, and an air outlet. 370.
  • the sensor 360 is provided to be connected to the flow path 330 inside the body 310. Specifically, the sensor 360 is provided to be connected to the flow path 330 on the upper surface of the lower substrate 314.
  • the blood injected into the blood inlet 320 passes through the flow path 330, and the blood cells are filtered by the first filter 340 and the second filter 350, and only the plasma reaches the sensor 360.
  • the sensor 360 reacts with the plasma, detects a specific component, and outputs a signal.
  • Sensor 360 may be an optical sensor.
  • Sensor 360 includes a color reagent, which reacts with a specific substance in the plasma to cause a color change.
  • the sensor 360 may further include an opening 362. Opening 362 exposes sensor 360 to the outside.
  • the opening 362 is provided on the upper surface of the body 310, and specifically, passes through the upper and lower surfaces of the upper substrate 312.
  • the meter may check the color change of the sensor 360 through the opening 362.
  • the specific component in the plasma may be quantitatively analyzed according to the degree of color change of the sensor 60.
  • the user may visually check the color change of the sensor 360 through the opening 362.
  • the air outlet 370 is provided on the upper surface of the body 310. Specifically, the air outlet 372 penetrates the upper and lower surfaces of the upper substrate 312 and is connected to the rear end of a portion of the flow path 330 provided with the second filter 350.
  • air in the flow path 330 of the portion where the second filter 350 is provided may be discharged to the outside through the air outlet 370. . Therefore, it is possible to prevent the generation of voids in the flow path 330 of the portion provided with the second filter 350.
  • the blood since the blood flow is not disturbed by the voids, the blood can move quickly to the sensor 360.
  • a second air outlet that connects to the sensor 360 need not be provided. Air in a portion where the sensor 360 is provided may be discharged to the outside through the opening 362. Therefore, it is possible to prevent the generation of voids in the area provided with the sensor 360, the blood can be quickly moved to the entire sensor 360 without the interference of the void.
  • the biosensor chip 400 includes a body 410, a blood inlet 420, a flow path 430, a first filter 440, a second filter 450, a sensor 460, and an air outlet. 470.
  • the sensor 460 is provided to be connected to the flow path 430 inside the body 410. Specifically, the sensor 460 is provided to be connected to the flow path 430 on the upper surface of the lower substrate 414.
  • the blood injected into the blood inlet 420 passes through the flow path 430, and the blood cells are filtered by the first filter 440 and the second filter 450, and only the plasma reaches the sensor 460.
  • Sensor 460 reacts with the plasma and detects specific components.
  • Sensor 460 may be an electrical sensor.
  • Sensor 460 includes an assay reagent, which reacts with a particular substance in the plasma.
  • the sensor 460 may further include an electrode 462, an opening 464, and an absorbent pad 490.
  • the electrode 462 is formed of at least one pair, and is provided to be connected to the sensor 460 inside the body 410.
  • the electrode 462 may be inserted into the upper surface of the lower substrate 414 to be connected to the sensor 460.
  • One end of the electrode 462 is connected to the sensor 460.
  • the electrode 462 may be made of a metal material. Examples of the metal material 462 include gold and copper.
  • the opening 464 exposes the other end of the electrode 462 opposite to the one end to the outside.
  • the opening 464 is provided on the upper surface of the body 410, and specifically, passes through the upper and lower surfaces of the upper substrate 412.
  • the electrode 462 exposed by the opening 464 may be connected to the socket device of the meter when the biosensor chip 400 is inserted into the meter.
  • the absorption pad 490 is provided to be connected to the sensor 460 inside the body 410.
  • the absorption pad 490 may be inserted into the upper surface of the lower substrate 414 to be connected to the sensor 460.
  • the absorbent pad 490 is connected to the other end of the sensor 460 opposite to one end of the sensor 460 connected to the flow path 430.
  • Absorption pad 490 absorbs the remaining reaction residue after the plasma reacts with sensor 460. When the reaction residual liquid is absorbed in the absorption pad 490, the sensor 460 maintains a dry state.
  • the electrical signal is input to the dry sensor 460 through the electrode 462, and the measuring device reads the electrical signal conveyed through the sensor 460 to the plasma. You can quantitatively analyze your specific components.
  • the reaction time of the electrode 462 may be adjusted by adjusting the width of the region in which the sensor 460 is inserted. For example, when the width of the region in which the sensor 460 is inserted is narrow, the plasma flow rate is fast, so that the electrodes 462 may react almost simultaneously. As another example, when the area where the sensor 460 is inserted is wide, the plasma flow rate is slow, so that the electrodes 462 may sequentially react.
  • the biosensor chip 400 includes a body 510, a blood inlet 520, a flow path 530, a first filter 540, a second filter 550, a sensor 560, and an air outlet. 570.
  • each sensor 560 may be any one of an electrochemical sensor, an optical sensor, and an electrical sensor.
  • the sensor 560 may be made of only one of electrochemical sensors, optical sensors, and electrical sensors, or may include a combination thereof. Therefore, since the biosensor chip 500 has a plurality of sensors 560, multiple measurements of the plasma may be performed.
  • the biosensor chip according to the present invention separates blood cells using the first filter and the second filter, the blood cells can be accurately separated from the blood.
  • the flow path is connected from the blood inlet to the sensor, the blood may be delivered to the sensor without external power through the flow path according to the capillary phenomenon. Therefore, separation of the blood cells and detection of the sensor can be performed without the external power.

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Abstract

La présente invention concerne une puce de biocapteur pouvant comprendre : un corps ; un trou d'injection de sang pratiqué sur la surface supérieure du corps, du sang étant injecté à travers ce dernier ; un canal de fluide réalisé à l'intérieur du corps pour se trouver en communication avec le trou d'injection de sang et permettant le déplacement du sang, qui a été injecté à travers le trou d'injection de sang, par un phénomène capillaire ; un premier filtre disposé dans le trou d'injection de sang et séparant de manière primaire des globules sanguins du sang ; un second filtre disposé dans le canal de fluide et séparant de manière secondaire des globules sanguins du sang ; et un capteur disposé à l'extrémité du canal de fluide à l'intérieur du corps et détectant un constituant spécifique tout en réagissant avec le plasma sanguin obtenu par séparation des globules sanguins du sang. Puisque le sang passe à travers les deux filtres, la puce de biocapteur peut séparer avec précision les globules sanguins.
PCT/KR2014/004413 2013-07-17 2014-05-16 Puce de biocapteur WO2015008927A1 (fr)

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Application Number Priority Date Filing Date Title
KR10-2013-0084058 2013-07-17
KR20130084058A KR20150009745A (ko) 2013-07-17 2013-07-17 바이오 센서 칩

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KR20180099964A (ko) * 2017-02-27 2018-09-06 (주)오상헬스케어 혈액 분석용 스트립
JP7005780B2 (ja) * 2017-11-21 2022-01-24 ビービービー インコーポレイテッド バイオセンサ
KR102469501B1 (ko) * 2020-10-30 2022-11-23 주식회사 네오나노텍 나노입자 생성을 위한 미세유체 칩 및 그 제조방법
KR20240010785A (ko) 2022-07-18 2024-01-25 주식회사 큐에스택 비색 바이오 센서

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