WO2015005885A1 - Surface preparation method by atmospheric plasma system for biomolecule immobilization and obtained surface by this method - Google Patents

Surface preparation method by atmospheric plasma system for biomolecule immobilization and obtained surface by this method Download PDF

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Publication number
WO2015005885A1
WO2015005885A1 PCT/TR2014/000246 TR2014000246W WO2015005885A1 WO 2015005885 A1 WO2015005885 A1 WO 2015005885A1 TR 2014000246 W TR2014000246 W TR 2014000246W WO 2015005885 A1 WO2015005885 A1 WO 2015005885A1
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Prior art keywords
plasma
precursor
biomolecule immobilization
amine functionalized
functionalized surface
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PCT/TR2014/000246
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French (fr)
Inventor
Ebru AKDOĞAN
Levent MERGEN
Gonca BİLGE
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Deltamed Yasam Bi̇li̇mleri̇ Ve Plazma Teknoloji̇leri̇ Ar-Ge Sanayi̇ Ve Ti̇caret Anoni̇m Si̇rketi̇
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Publication of WO2015005885A1 publication Critical patent/WO2015005885A1/en

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    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05H—PLASMA TECHNIQUE; PRODUCTION OF ACCELERATED ELECTRICALLY-CHARGED PARTICLES OR OF NEUTRONS; PRODUCTION OR ACCELERATION OF NEUTRAL MOLECULAR OR ATOMIC BEAMS
    • H05H1/00—Generating plasma; Handling plasma
    • H05H1/24—Generating plasma
    • H05H1/26—Plasma torches
    • H05H1/32—Plasma torches using an arc
    • H05H1/42—Plasma torches using an arc with provisions for introducing materials into the plasma, e.g. powder, liquid
    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05H—PLASMA TECHNIQUE; PRODUCTION OF ACCELERATED ELECTRICALLY-CHARGED PARTICLES OR OF NEUTRONS; PRODUCTION OR ACCELERATION OF NEUTRAL MOLECULAR OR ATOMIC BEAMS
    • H05H1/00—Generating plasma; Handling plasma
    • H05H1/24—Generating plasma
    • H05H1/26—Plasma torches
    • H05H1/32—Plasma torches using an arc
    • H—ELECTRICITY
    • H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05H—PLASMA TECHNIQUE; PRODUCTION OF ACCELERATED ELECTRICALLY-CHARGED PARTICLES OR OF NEUTRONS; PRODUCTION OR ACCELERATION OF NEUTRAL MOLECULAR OR ATOMIC BEAMS
    • H05H1/00—Generating plasma; Handling plasma
    • H05H1/24—Generating plasma
    • H05H1/26—Plasma torches
    • H05H1/30—Plasma torches using applied electromagnetic fields, e.g. high frequency or microwave energy

Definitions

  • This invention is related with a method in which functional amine groups are occured on every kind of materials' surfaces like ceramic, polymeric, metallic or composit surfaces modififying with atmospheric plasma system and obtaining appropriate surface for biomolecule immobilization by this method.
  • Polymeric, ceramic, metallic or composit materials can be changed physically and chemically by surface modification techniques and can be made appropriate for different application area.
  • Materials can be made appropriate for biomolecule immobilization by changing some properties like surface energy, adhesion force, wettability, surface roughness, surface functional groups and hardness so they can be used at wide application area.
  • biosensor systems In medical, enviroment, pharmacology and food industries frequently used biosensor systems, biofuel cells, in-vivo or ex-vivo biomaterials, packed bed columns for affinity chromotographies are prepared by immobilization of an active biomolecule like enzyme, antibody, peptide, receptor, organel, DNA, microorganism on ceramic, metallic, polymeric or composite surfaces. Thanks to this, materials can be used for diagnosis, process monitoring, extraction, purifying and the other application area.
  • biomolecule immobilization on carrier surfaces Most frequently used methods are adsorbtion, entrapment, covalent bounding, cross linking or combining these methods.
  • Preferred method varies according to carrier surface, biomolecule species and the purpose of study.
  • Carboxylic acid (- COOH) and amine (-NH2) groups are related with biological applications because these functional groups are in the structure of amino acids.
  • Carboxylic acid groups of amino acids can be cross-linked with amine groups on the surfaces by modifying carrier surfaces with amine groups. In the surface preperation for biomolecule immobilization occuring of amine groups on the surfaces is used frequently. Aldehydes and epoxy groups can be bounded to amine grafted surfaces. In addition to biomolecule immobilization amine functionalized surfaces are used for decreasing C0 2 emmision ratio.
  • the methods for grafting of functional groups are generally adsorbtion, photo- immobilization, gamma activation, chemical modification, ionized radiation (plasma treatment, ion beam, laser).
  • Chemical methods can be listed as dipping, self assembling monolayer (SAM) and spinning.
  • SAM self assembling monolayer
  • the most frequently used method in these methods is SAM.
  • amine containing chemicals like 3-aminoprophyl trietoxysilane (APTS), 3-aminoprophyl trimetoxysilane (APTMS), aminoprophyl trietoxysilane (APTES), 1 1-aminoundecan-l -thiol hydrochloride (AUDT) are applied to carrier surfaces.
  • APTS 3-aminoprophyl trietoxysilane
  • APITMS 3-aminoprophyl trimetoxysilane
  • APTES aminoprophyl trietoxysilane
  • 1 1-aminoundecan-l -thiol hydrochloride (AUDT) are applied to carrier surfaces.
  • This method is frequently used because it is simple but there are many process parameters like pH, temperature, solvent and concentration and it is difficult to control the process.
  • UV stimulating laser is also used for functionalization of nylon materials.
  • nylon materials When the nylon materials is excited amide groups in the structure transform to amine groups and functional amine groups are occured on the surfaces. But it is not frequently used method because of high cost of laser equipment.
  • the aim of this invention is occuring functional amino groups by plasma system on the surfaces and proceeding a surface preparing method for biomolecule immobilization.
  • the other aim of this invention is proceeding a homogenuous surface preparing method by atmospheric plasma system on the ceramic, metallic, polymeric or compostie 3D surfaces for biomolecule immobilization.
  • the other aim of the invention is proceeding a surface preparing method by atmospheric plasma system in a short time for biomolecule immobilization.
  • the other aim of the invention is proceeding a cheap surface preperation method by plasma system for biomolecule immobilization.
  • the other aim of the invention is proceeding a biocompatible surface preperation method by plasma system for biomolecule immobilization.
  • Plasma polymerisation process used to achieve above mentioned goals is in figures. These figures are:
  • FIG. 1 Schematic representation of plasma polymerisation system.
  • Figure-2 Flow chart for obtaining a surface for biomolecule immobilization.
  • the parts in figure 1 are numbered and the corresponding explanation of the parts is given below:
  • the plasma polymerization system (1) used is for obtaining a surface with prementioned properties works at atmospheric pressure and its simplest form consist of;
  • At least one precursor injection channel (9) that feeds the precursor used to modify the material surface (A),
  • At least one power supply (1) that supplies the energy required to the . system (1).
  • the method for developed obtaining a surface for biomolecule immobilization (100) using a plasma polymerisation system consists of the following steps:
  • the material to be modified is placed at the plasma polymerisation system (101). Then, the plasma forming gas is fed to the system through the plasma gas feeding channel (3), (102).
  • the plasma gas feeding channel (3) 102
  • at least one of nitrogen, oxygen, air, helium, argon, hydrogen or C0 2 gases is used as the plasma forming gas.
  • Plasma gas can be fed to the system (102) with a flow rate of 1-100 L/min at 1-10 bar pressure.
  • a doping gas can be fed to the system (103) through the doping gas feeding channel (4).
  • the doping gas can be at least one of oxygen, hydrogen, nitrogen, CF 4 , CH 4 or C0 2 gases.
  • Doping gas can be fed to the system with a flow rate of 0-100 L/min at 0,01-10 bar and the percentage of doping gas inside plasma gas is %0,001-%51.
  • a precursor is also fed to the system through precursor injection channel (7) with the help of a carrier gas and a peristhaltic pump (104).
  • carrier gas is the same as the plasma forming gas.
  • the flow rate of the carrier gas is between 0,01-100 L/min.
  • the feeding rate of the precursor to the system is between 0,01-100 mL/min.
  • Amine containing chemicals are used as precursor.
  • plasma is ignited in the electrode (5) by supplying the required power from the power supply (10), (105).
  • the working frequency of the power supply (10) is between 1-100 kHz.
  • Material (A) is modified by moving the probe (2) that contains the electrode (5) on its surface at a distance of 0,1-50 mm and at a speed of 0-500 mm/sec (106).
  • the surface to be modified is moved under the non-moving probe at a speed of 1-1000 mm/sec.
  • the moving-coating cycle is repeated 1 to 100 times until a coating at a desirable thickness or property is obtained.
  • both the probe and the material are fixed and plasma is generated on the surface for 0,1 to 1000 minutes. During plasma, the power fed to the system (1) is between 0,1-100 kVA.
  • the atmospheric plasma modification is applied to ceramic, polymeric or composite materials which can be used on different fields like medical, environment, pharmacology and food as biosensor systems, biofuel cells, in-vivo and ex-vivo implants, packed bed columns on affinity chromatograms etc.

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  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Plasma & Fusion (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Electromagnetism (AREA)
  • Materials For Medical Uses (AREA)

Abstract

This invention provides a method for functionalisation of surfaces using a plasma polymerization system (1), such as an atmospheric plama torch. The method comprises providing a surface to functionalise to system(l)(101), feeding of plasma gas to system (1) (102), feeding of doping gas to system (1)(103), feeding of polymer precursor to system (1) (104), generating a plasma (105), coating the surface (A) (106), obtaining of precursor coated material surface (A) (107) and it works at atmospheric conditions. This invention is related with amino functionalizing of ceramic, metallic, polymeric or composite materials' surfaces and perparing of a surface for biomolecule immobilization.

Description

DESCRIPTION
SURFACE PREPERATION METHOD BY ATMOSPHERIC PLASMA SYSTEM FOR BIOMOLECULE IMMOBILIZATION AND OBTAINED SURFACE BY THIS METHOD
Field of the Invention
This invention is related with a method in which functional amine groups are occured on every kind of materials' surfaces like ceramic, polymeric, metallic or composit surfaces modififying with atmospheric plasma system and obtaining appropriate surface for biomolecule immobilization by this method.
Background of the Invention
Surfaces of polymeric, ceramic, metallic or composit materials can be changed physically and chemically by surface modification techniques and can be made appropriate for different application area. Materials can be made appropriate for biomolecule immobilization by changing some properties like surface energy, adhesion force, wettability, surface roughness, surface functional groups and hardness so they can be used at wide application area.
In medical, enviroment, pharmacology and food industries frequently used biosensor systems, biofuel cells, in-vivo or ex-vivo biomaterials, packed bed columns for affinity chromotographies are prepared by immobilization of an active biomolecule like enzyme, antibody, peptide, receptor, organel, DNA, microorganism on ceramic, metallic, polymeric or composite surfaces. Thanks to this, materials can be used for diagnosis, process monitoring, extraction, purifying and the other application area.
Various methods are developed for biomolecule immobilization on carrier surfaces. Most frequently used methods are adsorbtion, entrapment, covalent bounding, cross linking or combining these methods. Preferred method varies according to carrier surface, biomolecule species and the purpose of study.
To immobilize the biomolecule on polymeric, metallic, ceramic or composite material surfaces by chemical methods like cross linking and covalent bounding carrier surfaces must be functionalized. For this purpose various methods are used. The basic principle of these methods is based on occuring of chemical linkage between biomolecule and carrier surface. In general functional groups contain carboxyl (-COOH), amine (-NH2), epoxi (- C2H30), pentafluorophenyl (-C6H5) hydroxyl (-OH) and sulfhydryl (-SH) groups. For the immobilization of biomolecule on carrier surface chemically on the surface there must be at least one species functional group. Carboxylic acid (- COOH) and amine (-NH2) groups are related with biological applications because these functional groups are in the structure of amino acids. Carboxylic acid groups of amino acids can be cross-linked with amine groups on the surfaces by modifying carrier surfaces with amine groups. In the surface preperation for biomolecule immobilization occuring of amine groups on the surfaces is used frequently. Aldehydes and epoxy groups can be bounded to amine grafted surfaces. In addition to biomolecule immobilization amine functionalized surfaces are used for decreasing C02 emmision ratio.
The methods for grafting of functional groups are generally adsorbtion, photo- immobilization, gamma activation, chemical modification, ionized radiation (plasma treatment, ion beam, laser).
Chemical methods can be listed as dipping, self assembling monolayer (SAM) and spinning. The most frequently used method in these methods is SAM. In this method amine containing chemicals like 3-aminoprophyl trietoxysilane (APTS), 3-aminoprophyl trimetoxysilane (APTMS), aminoprophyl trietoxysilane (APTES), 1 1-aminoundecan-l -thiol hydrochloride (AUDT) are applied to carrier surfaces. This method is frequently used because it is simple but there are many process parameters like pH, temperature, solvent and concentration and it is difficult to control the process. Furthermore low coating stability is another disadvantage for this method. In these methods surfaces can be destroyed because of using strong reducing/oxidizing chemical agents. Huge amount of chemical using, solvent using, needing of several process steps, incubation time are the other disadvantages of the method.
To overcome the wet chemistry's disadvantages variouns methods were developed. These methods are called dry modification techniques. These methods can be listed; flame, corona, UV, gamma radiation, electron current irradiation, ion beam, plasma and laser applications. Plasma treatments are frequently used to occur functional amine groups on the surface. In plasma treatments amine groups can be occured on the surfaces by using process gasses like ammonia and rc-heptylamine and also amine containing monomers like allylamine. The other method is grafting of amine groups on the surfaces by applicating UV at ammonia atmosphere. But colour pigments and coatings in the polymer structure restrict the UV application area. Pigments adsorb UV and interfere. Furthermore it is needed that avoiding of UV emmision in the field, protecting of eyes and skin.
UV stimulating laser is also used for functionalization of nylon materials. When the nylon materials is excited amide groups in the structure transform to amine groups and functional amine groups are occured on the surfaces. But it is not frequently used method because of high cost of laser equipment.
Summary of the Invention The aim of this invention is occuring functional amino groups by plasma system on the surfaces and proceeding a surface preparing method for biomolecule immobilization.
The other aim of this invention is proceeding a homogenuous surface preparing method by atmospheric plasma system on the ceramic, metallic, polymeric or compostie 3D surfaces for biomolecule immobilization.
The other aim of the invention is proceeding a surface preparing method by atmospheric plasma system in a short time for biomolecule immobilization.
The other aim of the invention is proceeding a cheap surface preperation method by plasma system for biomolecule immobilization.
The other aim of the invention is proceeding a biocompatible surface preperation method by plasma system for biomolecule immobilization.
Detailed Description of the Invention
Plasma polymerisation process used to achieve above mentioned goals is in figures. These figures are:
Figure!- 1 Schematic representation of plasma polymerisation system.
Figure-2 Flow chart for obtaining a surface for biomolecule immobilization. The parts in figure 1 are numbered and the corresponding explanation of the parts is given below:
1. Atmospheric plasma system
2. Probe
3. Feeding channel for the plasma gas
4. Feeding channel for the dopping gas
5. Electrode
6. Dielectric material
7. Potantial zone
8. Plasma refraining zone
9. Precursor injection channel
10. Power supply
A. Substrate surface
R. Discharge
The plasma polymerization system (1) used is for obtaining a surface with prementioned properties works at atmospheric pressure and its simplest form consist of;
At least one probe (2) where the plasma is discharged (R) and that moves on top of the material to be modified (A),
At least one plasma gas feeding channel (3) inside the probe (2) where the plasma forming gas is fed, - At least one doping gas feeding channel (4) inside the probe (2) where at least two different doping gases are fed,
- At least one electrode (5) that forms plasma,
- At least one dielectric material (6) that surrounds the electrode (4), - A potential zone (7) where a potential difference is produced,
- A plasma restriction zone (8) that contains the plasma inside the probe (2) and that prevents the plasma to extinguish after discharge,
At least one precursor injection channel (9) that feeds the precursor used to modify the material surface (A),
At least one power supply (1) that supplies the energy required to the . system (1).
The method for developed obtaining a surface for biomolecule immobilization (100) using a plasma polymerisation system that is described above consists of the following steps:
Placing the material to be modified in the system (1), (101), Feeding the plasma gas into the system (1), (102), Feeding the doping gas in to the system (1), (103), Feeding the precursor in to the system (1), (104), - Plasma ignition (105),
Modification of the material surface (A), (106), - 1 Obtaining a surface modified material (A) with the precursor (107).
In the method for obtaining a surface for biomolecule immobilization (100); the material to be modified is placed at the plasma polymerisation system (101). Then, the plasma forming gas is fed to the system through the plasma gas feeding channel (3), (102). In the preferred application of the invention, at least one of nitrogen, oxygen, air, helium, argon, hydrogen or C02 gases is used as the plasma forming gas. Plasma gas can be fed to the system (102) with a flow rate of 1-100 L/min at 1-10 bar pressure. In addition to the plasma forming gas, a doping gas can be fed to the system (103) through the doping gas feeding channel (4). In the preferred application of the invention the doping gas can be at least one of oxygen, hydrogen, nitrogen, CF4, CH4 or C02 gases. Doping gas can be fed to the system with a flow rate of 0-100 L/min at 0,01-10 bar and the percentage of doping gas inside plasma gas is %0,001-%51.
A precursor is also fed to the system through precursor injection channel (7) with the help of a carrier gas and a peristhaltic pump (104). In the preferred application of the invention, carrier gas is the same as the plasma forming gas. The flow rate of the carrier gas is between 0,01-100 L/min. The feeding rate of the precursor to the system is between 0,01-100 mL/min. Amine containing chemicals are used as precursor. These chemicals are allylamine, ethylenediamine, 1,2-diaminopropane, N-methylenediamine, 1 ,4-diaminobutane, 3-(methylamino)propylamine, Ν,Ν'- dimethylethylenediamine, N-ethyl ethylenediamine, N-methyl-1,3- diaminopropane, 1 -dimethylamino-2-propilamine, 1 -dimethylamino-2- propilamine, cadaverine, N,N'-dimethyl-l,3-propandiamine, Ν,Ν,Ν',Ν'- tetramethyldiaminomethane, Ν,Ν,Ν'-trimethylethylendiamine, N- isopropilethylendiamine, N-propilethylendiamine, N-(2-aminoetil)-l,3- propandiamine, hegzamethylendiamine, Ν,Ν'-diethylethylendiamine, Ν,Ν,Ν',Ν'- tetramethylethylendiamine, Ν,Ν,Ν'-trimethyl- 1 ,3 -propandiamine, N,N- diethylethylendiamine, N,N-dimethyl-N'-ethylethylendiamine, N- buthylethylendiamine, N-isopropyl-l,3-propandiamine, N-propil-1 ,3- propandiamine, bis(3-aminoprophyl)amine, triethylentetramine, 1,3- bis(etilamino)propane, 1 ,7-diaminoheptane, 3-(diethylamino)propylamine, N,N'-diethyl-l,3-propandiamine, N,N,2,2-tetramethyl-l,3-propandiamine, N,N,N',N'-tetramethyl-l ,3 -propandiamine, N,N-diethyl-N'- methylenethylendiamine, 3,3'-diamino-N-methyldipropilamine, Nl- isopropyldiethylentriamine, tris(dimethylamino)methane, N,N'-bis(2-aminoethyl)- 1,3 -propandiamine, trans-N,N,N',N'-tetramethyl-2-butene-l,4-diamine, 1,8- diaminooctane, Ν,Ν'-dimethyl- 1 ,6-hegzanediamine, Ν,Ν,Ν',Ν'-tetramethyl- 1,3- butanediamine, Ν,Ν,Ν'-trietiletilendiamin, N-hegzylethylendiamine, Bis[2-(N,N- dimethylamino)ethyl] ether, Ν,Ν-diethyldiethylentriamine, N,N- dimethyldipropylentriamine, 1 ,2-bis(3-aminopropylamino)ethane, tetraethylenpentamine, 2-amino-5-diethylaminopentane, 1 , 10-diaminodecane, pentaethylenhegzamine veya 1 ,12-diaminododecane. At least one chemical among these can be used as precursor.
With the introduction of the plasma gas, doping gas and precursor into the system (1), plasma is ignited in the electrode (5) by supplying the required power from the power supply (10), (105). The working frequency of the power supply (10) is between 1-100 kHz.
Material (A) is modified by moving the probe (2) that contains the electrode (5) on its surface at a distance of 0,1-50 mm and at a speed of 0-500 mm/sec (106). In another application of the invention, the surface to be modified is moved under the non-moving probe at a speed of 1-1000 mm/sec. For both applications, the moving-coating cycle is repeated 1 to 100 times until a coating at a desirable thickness or property is obtained. In another application, both the probe and the material are fixed and plasma is generated on the surface for 0,1 to 1000 minutes. During plasma, the power fed to the system (1) is between 0,1-100 kVA.
When the plasma cycle is completed and the surface is modified (106), a material modified/coated with the precursor and that is amine functionalized, homogenous and that is convenient for biomolecule immobilization is obtained (107).
In the method described (100) in this invention, the atmospheric plasma modification is applied to ceramic, polymeric or composite materials which can be used on different fields like medical, environment, pharmacology and food as biosensor systems, biofuel cells, in-vivo and ex-vivo implants, packed bed columns on affinity chromatograms etc.

Claims

1. A method for obtaining a amine functionalized surface for biomolecule immobilization, in its simplest form, that requires an atmospheric pressure plasma system that consists of;
- a probe (2) that moves onto the material surface (A) in which plasma is ignited (R), - at least one plasma gas feeding channel (3) inside the probe (2) where the plasma forming gas is fed
- at least one doping gas feeding channel (4) inside the probe (2) where at least two different doping gases are fed,
- at least one electrode (5) that ignites the plasma, - at least one dielectric material (6) surrounding the electrode (4),
- a potential zone (7) where a potential difference is produced,
- a plasma restriction zone (8) that contains the plasma inside the probe (2) and that prevents the plasma to extinguish after discharge,
- at least one precursor injection channel (9) that feeds the precursor used to modify the material surface (A),
- at least one power supply (1) that supplies the energy required to the system (1).
And that is characterised with the following steps; - placing the material to be modified into the plasma system (1), (101), - feeding plasma forming gas into the system (1), (102),
- feeding the doping gas in to the system (1), (103),
- feeding the precursor in to the system (1), (104),
- plasma ignition (105), - modification of the material surface (A), (106),
- A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as modifying material's surface (A) with the precursor (107).
2. A method for obtaining amine functionalized surface for biomolecule immobilization (100) as defined in claim 1 wherein the precursor feeding step (102) to the system (1), the precursor is at least one of oxygen, air, argon, hydrogen or carbondioxide.
3. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above at the step of feeding of precursor (102) to system (1), plasma gas is feeded to probe (2) between 1-100 L/min flow rate and at 1-10 bar pressure.
4. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined by using of at least two gasses from oxygen, hydrogen, nitrogen, CF4, CH4,C02 as dopping gas at the step of feding of dopping gas (103) to system (1).
5. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above at the step of feeding of dopping gas (103) to system (1), plasma gas is feeded to probe (2) between 1-100 L/min flow rate and at 0,01 -10 bar pressure.
6. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the step of feeding of dopping gas (103) to system (1) volumetric ratio of dopping gas in plasma gas is %0,001-%51.
7. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the step of feding of precursor (104) to the system (1), by feding of precursor into the probe (2) with a carrier gas through a peristhaltic pump.
8. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the step of feding of precursor (104) to the system (1), the gas which carries the precursor is plasma gas.
9. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the step of feding of precursor (104) to the system (1), flow rate of carrier gas which carries the precursor is between 0,01-100 L/min.
10. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the step of feding of precursor (104) to the system (1), flow rate of feding of precursor is between 0,01 - 100 mL/min.
11. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the step of feding of precursor (104) to the system (1), precursor consist of amine comtaining chemicals.
12. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system as in claim 11 which is defined as above claims at the step of feding of precursor (104) to the system (1), precursor contains at least one chemical among these chemicals; allylamine, ethylenediamine, 1,2-diaminopropane, N-methylenediamine, 1,4-diaminobutane, 3-(methylamino)propylamine, N,N '-dime thy lethylenediamine, N- ethylethylenediamine, N-methyl- 1 ,3 -diaminopropane, 1 -dimethyl amino-2- propilamine, 1 -dimethylamino-2-propilamine, cadaverine, N,N'-dimethyl-l,3- propandiamine, Ν,Ν,Ν',Ν'-tetramethyldiaminomethane, Ν,Ν,Ν'- trimethylethylendiamine, N-isopropilethylendiamine, N-propilethylendiamine, N- (2-aminoetil)-l,3-propandiamine, hegzamethylendiamine, Ν,Ν'- diethylethylendiamine, Ν,Ν,Ν',Ν'-tetramethylethylendiamine, Ν,Ν,Ν'-trimethyl- 1,3-propandiamine, Ν,Ν-diethyl ethyl endiamine, N,N-dimethyl-N'- ethylethylendiamine, N-buthylethylendiamine, N-isopropyl- 1 ,3-propandiamine, N-propil-l,3-propandiamine, bis(3-aminoprophyl)amine, triethylentetramine, 1,3- bis(etilamino)propane, 1 ,7-diaminoheptane, 3-(diethylamino)propylamine, • N,N'-diethyl-l,3-propandiamine, N,N,2,2-tetramethyl-l,3-propandiamine, N,N,N',N'-tetramethyl-l ,3-propandiamine, N,N-diethyl-N'- methylenethylendiamine, 3,3'-diamino-N-methyldipropilamine, Nl- isopropyldiethylentriamine, tris(dimethylamino)methane, N,N'-bis(2-aminoethyl)- 1 ,3-propandiamine, trans-N,N,N',N'-tetramethyl-2-butene- 1 ,4-diamine, 1 ,8- diaminooctane, N,N'-dimethyl-l ,6-hegzanediamine, N,N,N',N'-tetramethyl-l ,3- butanediamine, Ν,Ν,Ν'-trietiletilendiamin, N-hegzylethylendiamine, Bis[2-(N,N- dimethylamino)ethyl] ether, Ν,Ν-diethyldiethylentriamine, N,N- dimethyldipropylentriamine, 1 ,2-bis(3-aminopropylamino)ethane, tetraethylenpentamine, 2-amino-5-diethylaminopentane, 1 , 10-diaminodecane, pentaethylenhegzamine veya 1,12-diaminododecane
13. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined at the plasma occuring (105) step by opening the power supply (10) and starting the system (1) and occuring of the plasma inside the electrode (5) (100).
14. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the plasma occuring (105) step working frequency of the power supply (10) is between 1-100 kHz.
15. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the step of material coating (A) (106); distance from probe in which plasma occurs (2) to coated surface is between 0.1 -50 mm (100).
16. A method for obtaining amine functionalized surface for biomolecule immobilization (100) by atmospheric plasma system which is defined as above claims at the step of material coating (A) (106); speed of the probe in which plasma occurs (2) on the coated surface (A) is between 0-500 mm/sn (100).
17. A method for obtaining amine functionalized surface for biomolecule immobilization by atmospheric plasma system which is defined as in claims 1-15 at the step of material coating (A) (106); coated surface (A) moves under the probe with 0- 1000 mm/sn speed ( 100).
18. A method for obtaining amine functionalized surface for biomolecule immobilization by atmospheric plasma system which is defined as in claims 16 and 17 at the step of material coating (A) (106) movement repeats 1-100 times (100).
19. A method for obtaining amine functionalized surface for biomolecule immobilization by atmospheric plasma system which is defined as in claims 1-15 at the step of material coating (A) (106) material's surface is waited under the probe for 0,1-1000 min by holding the coated surface (A) and plasma occuring probe (2) steady (100).
20. A method for obtaining amine functionalized surface for biomolecule immobilization by atmospheric plasma system which is defined as at the step of material coating (A) (106) feeded power to the system (1) by power supplier (10) is 0.1-100 kVA (100).
21. A method for obtaining amine functionalized surface for biomolecule immobilization by atmospheric plasma system which is defined as at the step of obtaining of precursor coated material's surface (A) (107); modifying the surface (A) with functional amine groups (100).
22. An amino functionalized surface for biomolecule immobilization that is obtained as described in any of the claims above.
23. An amine functionalized surface for biomolecule immobilization as described in claim 22 that can be used in medical, enviroment, pharmacology and food area frequently used biosensor systems, biofuel cells, in-vivo or ex-vivo biomaterials, packed bed columns for affinity chromotographies
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Publication number Priority date Publication date Assignee Title
EP3650580A1 (en) * 2018-11-12 2020-05-13 Molecular Plasma Group SA Improved method for plasma immobilization of a biomolecule to a substrate via a linking molecule
WO2020099434A1 (en) * 2018-11-12 2020-05-22 Molecular Plasma Group Sa Improved method for plasma immobilization of a biomolecule to a substrate via a linking molecule

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