WO2015004816A1 - Réservoir de culture de micro-algues - Google Patents

Réservoir de culture de micro-algues Download PDF

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Publication number
WO2015004816A1
WO2015004816A1 PCT/JP2013/069713 JP2013069713W WO2015004816A1 WO 2015004816 A1 WO2015004816 A1 WO 2015004816A1 JP 2013069713 W JP2013069713 W JP 2013069713W WO 2015004816 A1 WO2015004816 A1 WO 2015004816A1
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WO
WIPO (PCT)
Prior art keywords
tank
microalgae
tanks
surface portion
surface section
Prior art date
Application number
PCT/JP2013/069713
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English (en)
Japanese (ja)
Inventor
西平隆
Original Assignee
Ｔｒａｎｓ Ａｌｇａｅ株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ｔｒａｎｓ Ａｌｇａｅ株式会社 filed Critical Ｔｒａｎｓ Ａｌｇａｅ株式会社
Priority to PCT/JP2013/069713 priority Critical patent/WO2015004816A1/fr
Publication of WO2015004816A1 publication Critical patent/WO2015004816A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel

Definitions

  • the present invention relates to a microalgal culture tank for producing biofuel.
  • biofuel is a fuel obtained from biomass and includes not only living organisms but also all by-products from metabolic activities such as animal excreta.
  • Bioethanol and biodiesel are also included as renewable energies different from fossil fuels. Bioethanol emits less carbon dioxide than fossil fuels, and has been in the limelight as a renewable energy source. It can be produced by alcohol fermentation of sugars such as sugarcane in plants and starch extracted from cereal grains such as corn. It can be obtained by fermentation.
  • Biodiesel can be obtained by reacting oil extracted from oil and fat crops such as rape blossoms and soybeans, or waste edible oil into alcohol and making it into fuel.
  • Biogas can be obtained using methane gas generated when fermenting food waste and livestock waste.
  • biofuels have attracted attention from the world because they absorb CO2 in the atmosphere and are extracted from fast-growing plants so that they can be mass-produced.
  • biofuels in order to obtain biofuel from cereals, there is a problem that a large area of land is required, resources are scattered in some countries, and there are large regional differences in the amount of resources.
  • biofuels expands, there is a problem that it may cause environmental problems due to emissions at the same time as the price of grains input to it increases.
  • a biofuel production method using microalgae has been proposed, and this biofuel uses a single cell organism that is highly proliferating and that performs photosynthesis.
  • This method is mainly forcing microalgae by injecting carbon dioxide into a reaction facility such as a large lake or a large pond, which increases initial investment costs and maintenance costs. There was a problem.
  • the productivity of microalgae is low compared to the large area of the reaction facility, and light is not transmitted effectively to the depth of the inside of the pond, resulting in a slow growth rate of microalgae.
  • the problem to be solved is that it requires a large area of land, raises the price of cereals, causes environmental problems due to emissions during production, and fails to accelerate the growth rate of microalgae .
  • the present invention does not cause an increase in the price of grains, and a large number of microalgae culture tanks are used in the culture facility to eliminate waste during production and the tank can be easily installed.
  • the initial investment costs and maintenance costs are reduced by allowing the light from the outside to reach the inside of the tank smoothly so that the growth rate of microalgae can be increased and the productivity can be increased. .
  • FIG. 1 is a perspective view illustrating a microalgae culture tank according to the present invention.
  • FIG. 2 is a plan view illustrating a tank for culturing microalgae according to the present invention.
  • FIG. 3 is a front view of a tank for culturing microalgae according to the present invention.
  • FIG. 4 is an exemplary diagram illustrating a process of harvesting microalgae cultured in a microalgae culture tank according to the present invention.
  • (Example) 5 is an enlarged cross-sectional view of a portion A shown in FIG. 6, 7 and 8 show mathematical figures for explaining the structure of the tank of the present invention.
  • FIGS. 6, 7, and 8 illustrate mathematical structures for explaining the structure of the tank.
  • An object on a figure is shown.
  • the truncated cone 50 is a solid object surrounded by an upper end surface 51, a lower end surface 52, and an outer peripheral surface 55.
  • the upper end surface 51 and the lower end surface 52 are both disk-shaped and parallel to each other, and a center line 57 passing through the centers of the upper end surface 51 and the lower end surface 52 can be set in these disks.
  • the upper end surface 51 and the lower end surface 52 intersect at a right angle, and if the diameter of the upper end surface 51 is R and the diameter of the lower end surface 52 is r, then R> r.
  • a truncated cone 50 is cut in the vertical direction in the figure on the left and right sides of a virtual plane 58 and a virtual plane 59 that are substantially parallel to each other and substantially parallel to the center line 57.
  • the truncated cone 50 is deformed into a substantially truncated cone 60.
  • the resulting truncated cone 60 is formed with a left surface 68 and a right surface 69 that are substantially hyperbolic in the left and right sides and substantially parallel to each other.
  • the substantially parallel left surface 68 and right surface 69 are cut so that the distance from the lower end surface 52 toward the upper end surface 51 is slightly separated from each other.
  • the distance between the left surface 68 and the right surface 69 is smaller than the maximum diameter R and the minimum diameter is larger than r. Therefore, the outer peripheral surface 55 is composed of a left outer surface 68, a right outer surface 69, and a front outer convex surface 70 and a rear outer convex surface 71, which are respectively located in the front and rear in the drawing.
  • a microalgae culture tank 1 is a substantially truncated cone having an open top, and is composed of a bottom surface portion 2 and a side surface portion 3 (corresponding to the lower end surface 52 and the outer peripheral surface 55 of FIG. 6).
  • Part 2 is disk-shaped.
  • the side surface portion 3 further includes the right surface portion 5, the left surface portion 6, the front portion cylindrical portion 8, and the rear portion cylindrical portion 9 (the left surface 68, the right surface 69, the front outward convex surface 70, and the rear outward portion in FIG. Each corresponding to the convex surface 71).
  • the right surface portion 5 and the left surface portion 6 are substantially parallel to each other across the open portion 10, and the right surface portion 5 and the left surface portion 6 are respectively connected to the left and right end portions of the bottom surface portion 2 and open from the bottom.
  • the distance from each other is gradually increased as it goes in the direction of the upper part.
  • the front cylindrical portion 8 and the rear cylindrical portion 9 face each other with the open portion 10 interposed therebetween, have outward convex shapes in opposite directions, and are continuous with the front and rear end portions of the end portion of the bottom surface portion 2, respectively.
  • Opposed parallel straight line portions 73 and parallel straight line portions 74 are formed at the upper ends of the right surface portion 5 and the left surface portion 6 of the tank 1, respectively, and the upper end portions of the front portion cylindrical portion 8 and the rear portion cylindrical portion 9 face each other.
  • Arc-shaped arcuate part 75 and arcuate part 76 are formed respectively.
  • the peripheral upper end 77 including the continuous parallel straight line portion 73 and the parallel straight line portion 74 and the arcuate portion 75 and the arcuate portion 76 is displayed in FIG. 2 when FIG. 1 is viewed from above.
  • the circumferential upper end 77 corresponds to a cross-sectional view in which the side surface portion 3 of the substantially truncated cone is a virtual plane parallel to the bottom surface portion 2 and is cut at the position of the height h of the tank 1 shown in FIG.
  • the cross-sectional view cut along the virtual plane is similar to the peripheral upper end 77 and has a small shape.
  • the microalgae culturing tank 1 having such a structure is made of polyethylene, is flexible, and can transmit light of most wavelengths.
  • elongated reinforcing bands 11 and 12 that are recessed inwardly when viewed from the outside are formed in the middle of the right surface portion 5 and the left surface portion 6 in parallel with the bottom surface portion 2.
  • the circumferential upper end portion 77 is formed with a reinforcing end portion 14 that is bent downward toward the inside of the open portion 10. As shown in FIG. 5 in which the portion A of FIG. 1 is enlarged, the reinforcing end portion 14 has a primary bent portion 15 formed inside and a secondary bent portion 16 formed again downward.
  • the strength of the upper portion of the tank 1 is increased against external force, and the cross-sectional area of the reinforcing end portion 14 is that of the reinforcing band 11 and the reinforcing band 12. It is formed slightly smaller than the cross-sectional area.
  • a space continuously surrounded by the bottom surface portion 2, the left surface portion 5, the right surface portion 6, the front partial cylindrical portion 8, and the rear partial cylindrical portion 9 forming the side surface portion 3 corresponds to the inner product of the tank 1.
  • the thickness of the polyethylene which is the material of the bottom surface portion 2 and the side surface portion 3 is determined empirically so that it can sufficiently withstand the external pressure when the inner space is filled with water or seawater for a long time.
  • the height h of the tank 1 is shorter than the maximum separation distance a between the arcuate part 75 and the arcuate part 76 shown in FIG. 2 and longer than the distance b between the parallel linear part 73 and the parallel linear part 74.
  • the upper end of the tank 1 is an open portion 10 that is open (corresponding to the opening 72 in FIG. 6), and liquid such as water containing microalgae can be injected (discharged).
  • the bottom part 2 of the other tank 1 is inserted one after another from the upper end opening part 10 of one tank 1 and overlapped to greatly reduce the collective volume of a large number of tanks 1 so that a large number of tanks 1 can be transported at a time.
  • the distance between the substantially parallel right surface portion 5 and the left surface portion 6 of one tank 1 is slightly larger than the diameter of the bottom surface portion 2 of the other tank 1, insertion is facilitated.
  • the microalgae culture tank 1 is constructed by installing it on a flattened and hardened floor on both sides in the length direction of the road 80 at the installation site.
  • Two tanks for cultivating microalgae 1 (for example, if the tank capacity is 10,000 L or more), a pair of tanks 79 in which the left surface portion 5 and the right surface portion 6 face each other in the vertical direction in the figure, A large number are arranged side by side in the length direction on both sides of 80.
  • each tank 1 is installed such that the left surface portion 5 and the right surface portion 6 are in contact with each other, and four rows of tank groups are formed along the both sides of the work road 80 in the length direction.
  • the maximum separation distance a between the front cylindrical portion 8 and the rear cylindrical portion 9 is longer than the distance b between the left surface portion 5 and the right surface portion 6 with respect to the open portion 10 of the above-described microalgae culture tank 1.
  • a large internal space is secured, and water and algae are put from here to nearly nine minutes. Since the flexible tank 1 tends to bulge outward due to the pressure of water and algae, the left tank 5 of one tank 1 and the right tank 6 of the other tank 1 are pressed against each other in the pair of tanks 79. However, at the same time, the front part cylindrical part 8 and the rear part cylindrical part 9 of the one and the other tanks 1 are both slightly spread outward. As schematically shown in FIG.
  • the left surface portion 5 of one tank 1 and the right surface portion 6 of the other tank 1 are more closely attached to each other. Can be maintained. Water containing single cell organisms that photosynthesize is stably stored. There is a function to increase productivity by increasing the growth rate of microalgae by allowing direct sunlight and external light to pass through the tank 1. Since the tank 1 for culturing microalgae has a substantially truncated cone shape, it is extremely stable as compared with a conventional tank in which the upper end portion and the bottom portion are formed in the same cylindrical shape or square tube shape.
  • the microalgae culture tank 1 made of polyethylene prevents corrosion and allows light from the outside to pass through smoothly.
  • zone 12 can prevent the deformation
  • microalgae culture tanks 1 arranged in four rows in the direction of the work road containing water containing microalgae are subjected to photosynthesis from the outside in the process of almost 24 hours, microalgae Will increase about twice.
  • the microalgae cultured in the microalgae culture tank 1 are harvested while moving the microalgae collection trailer 91 on the work road 80 as shown in FIG.
  • each net 95 is attached to a swing arm 94 that is made rotatable on a frame 93 on the trailer 91.
  • the trailer 91 On both sides of the work road 80, the trailer 91 is moved so that the four nets 95 are located above the tanks 1 of the respective paired tanks 79 arranged side by side. Therefore, each net 95 is moved down into the inside of each microalgae culturing tank 1 to be picked up and then pulled up. With this operation, the microalgae enter the raised net 95 and are harvested.
  • the swing arm 94 is rotated approximately 90 degrees so that the four nets 95 are positioned above the wide hopper 96 of the trailer 91.
  • the net 95 that has been swallowed opens, and the microalgae are dropped into the hopper 96 and collected.
  • the microalgae are dropped into the hopper 96 and collected.
  • the harvested microalgae can then be separated into water and microalgae by a post-process filter (not shown), and then compressed or administered with another flocculant to become a biofuel-containing material.
  • ⁇ Easy installation on the ground and floor of the microalgae cultivation site can reduce the initial installation cost, thereby further reducing the maintenance cost. Accelerate the growth of microalgae and expand its use for biodiesel production.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un réservoir (1) de culture de micro-algues dont le matériau est constitué de polyéthylène, doté de flexibilité et formé de façon à être sensiblement un cône tronqué. Ce cône sensiblement tronqué comprend une section (2) de surface inférieure en forme de disque et une section (3) de surface latérale, la section (3) de surface latérale étant conçue avec une section (6) de surface gauche, une section (5) de surface droite, une section (8) de cylindre partiel avant et une section (9) de cylindre partiel arrière à surfaces sensiblement parallèles. Pour transporter un grand nombre de réservoirs (1) avant la construction, les réservoirs peuvent se chevaucher grâce à leur forme de cône sensiblement tronqué et, par conséquent, le volume de chargement peut être fortement réduit, tandis qu'un grand nombre de réservoirs peut être expédié en même temps. Lors de l'érection sur un site de culture de micro-algues, une paire de réservoirs (70) peut maintenir une position dressée qui soit stable par rapport au vent et aux vibrations, en amenant des réservoirs adjacents en contact de surface les uns avec les autres, la section (6) de surface gauche et la section (5) de surface droite des réservoirs venant conjointement en butée. Après que les réservoirs (1) ont été remplis de micro-algues et d'eau et ont été installés, puisque le matériau du réservoir est du polyéthylène, la lumière solaire et ses rayons de chaleur pénètrent à l'intérieur des réservoirs, ce qui permet d'accélérer la croissance des micro-algues.
PCT/JP2013/069713 2013-07-12 2013-07-12 Réservoir de culture de micro-algues WO2015004816A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2013/069713 WO2015004816A1 (fr) 2013-07-12 2013-07-12 Réservoir de culture de micro-algues

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2013/069713 WO2015004816A1 (fr) 2013-07-12 2013-07-12 Réservoir de culture de micro-algues

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WO2015004816A1 true WO2015004816A1 (fr) 2015-01-15

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0315324A (ja) * 1989-05-16 1991-01-23 Sanofi Sa 藻類培養タンク及びこれを用いる方法
JPH11275994A (ja) * 1998-03-26 1999-10-12 Yanmar Diesel Engine Co Ltd 藻類の培養装置
JP2009539608A (ja) * 2006-06-14 2009-11-19 カーツ，マルコム，グレン 二酸化炭素固定のための方法および装置
WO2010116946A1 (fr) * 2009-04-06 2010-10-14 マイクロリソース株式会社 Dispositif de culture de micro-algues

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0315324A (ja) * 1989-05-16 1991-01-23 Sanofi Sa 藻類培養タンク及びこれを用いる方法
JPH11275994A (ja) * 1998-03-26 1999-10-12 Yanmar Diesel Engine Co Ltd 藻類の培養装置
JP2009539608A (ja) * 2006-06-14 2009-11-19 カーツ，マルコム，グレン 二酸化炭素固定のための方法および装置
WO2010116946A1 (fr) * 2009-04-06 2010-10-14 マイクロリソース株式会社 Dispositif de culture de micro-algues

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