WO2014210159A1 - Methods of modulating cftr activity - Google Patents
Methods of modulating cftr activity Download PDFInfo
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- WO2014210159A1 WO2014210159A1 PCT/US2014/044100 US2014044100W WO2014210159A1 WO 2014210159 A1 WO2014210159 A1 WO 2014210159A1 US 2014044100 W US2014044100 W US 2014044100W WO 2014210159 A1 WO2014210159 A1 WO 2014210159A1
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- optionally substituted
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- 0 *c1c(*)[o]nc1C(N(*)*=I)=O Chemical compound *c1c(*)[o]nc1C(N(*)*=I)=O 0.000 description 6
- QZZNATOUAVPEMF-UHFFFAOYSA-N CC(C)c1cc(C(NCC(c2ccncc2)N2CCOCC2)=O)n[o]1 Chemical compound CC(C)c1cc(C(NCC(c2ccncc2)N2CCOCC2)=O)n[o]1 QZZNATOUAVPEMF-UHFFFAOYSA-N 0.000 description 1
- STROVDLXNLEYGG-UHFFFAOYSA-N CC(C)c1cc(C(NCC(c2ncccc2)N2CCOCC2)=O)n[o]1 Chemical compound CC(C)c1cc(C(NCC(c2ncccc2)N2CCOCC2)=O)n[o]1 STROVDLXNLEYGG-UHFFFAOYSA-N 0.000 description 1
- IEMMBWWQXVXBEU-UHFFFAOYSA-N CC(c1ccc[o]1)=O Chemical compound CC(c1ccc[o]1)=O IEMMBWWQXVXBEU-UHFFFAOYSA-N 0.000 description 1
- FEOGXDXTDFICNS-UHFFFAOYSA-N CCCc1cc(C(NCC(CC)N2CC(c3ccc(C(CNC(c4n[o]c(-c5ccc[s]5)c4)=O)N4CCOCC4)[o]3)OCC2)=O)n[o]1 Chemical compound CCCc1cc(C(NCC(CC)N2CC(c3ccc(C(CNC(c4n[o]c(-c5ccc[s]5)c4)=O)N4CCOCC4)[o]3)OCC2)=O)n[o]1 FEOGXDXTDFICNS-UHFFFAOYSA-N 0.000 description 1
- KQWAZLUOYYQTIE-UHFFFAOYSA-N CCOC(c1n[o]c(-c2ccc[o]2)c1)=O Chemical compound CCOC(c1n[o]c(-c2ccc[o]2)c1)=O KQWAZLUOYYQTIE-UHFFFAOYSA-N 0.000 description 1
- KMVFIIBYOFOADI-UHFFFAOYSA-N CN(CCC1)C1c1ccc[o]1 Chemical compound CN(CCC1)C1c1ccc[o]1 KMVFIIBYOFOADI-UHFFFAOYSA-N 0.000 description 1
- YYWHNDSXZIIBQI-UHFFFAOYSA-N CSCc1ncc(CCNC(c2n[o]c(-c3ccccc3)c2)=O)[s]1 Chemical compound CSCc1ncc(CCNC(c2n[o]c(-c3ccccc3)c2)=O)[s]1 YYWHNDSXZIIBQI-UHFFFAOYSA-N 0.000 description 1
- YUGWEICZKZBDGT-UHFFFAOYSA-N O=C(c1n[o]c(-c2ccccc2)c1)NCCCN1CCOCC1 Chemical compound O=C(c1n[o]c(-c2ccccc2)c1)NCCCN1CCOCC1 YUGWEICZKZBDGT-UHFFFAOYSA-N 0.000 description 1
- KYEBQLCFQDPCBM-UHFFFAOYSA-N O=C(c1n[o]c(-c2ccccc2)c1)NCCN1CCOCC1 Chemical compound O=C(c1n[o]c(-c2ccccc2)c1)NCCN1CCOCC1 KYEBQLCFQDPCBM-UHFFFAOYSA-N 0.000 description 1
- IAHMVRCTECLFMG-UHFFFAOYSA-N O=Cc1n[o]c(-c2ccc[o]2)c1 Chemical compound O=Cc1n[o]c(-c2ccc[o]2)c1 IAHMVRCTECLFMG-UHFFFAOYSA-N 0.000 description 1
- MLWFYCMVBAIITM-UHFFFAOYSA-N OC(c1n[o]c(-c2ccc[o]2)c1)=O Chemical compound OC(c1n[o]c(-c2ccc[o]2)c1)=O MLWFYCMVBAIITM-UHFFFAOYSA-N 0.000 description 1
- PMAAVPFQCVXDAF-JOCQHMNTSA-N O[C@H](CC1)CC[C@@H]1NC(c1n[o]c(-c2ccccc2)c1)=O Chemical compound O[C@H](CC1)CC[C@@H]1NC(c1n[o]c(-c2ccccc2)c1)=O PMAAVPFQCVXDAF-JOCQHMNTSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/42—Oxazoles
- A61K31/422—Oxazoles not condensed and containing further heterocyclic rings
-
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- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/42—Oxazoles
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- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/4245—Oxadiazoles
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/425—Thiazoles
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
- A61K31/443—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with oxygen as a ring hetero atom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
- A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
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- A61K31/00—Medicinal preparations containing organic active ingredients
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- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/47—Quinolines; Isoquinolines
- A61K31/4704—2-Quinolinones, e.g. carbostyril
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/4965—Non-condensed pyrazines
- A61K31/497—Non-condensed pyrazines containing further heterocyclic rings
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- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
- A61K31/5375—1,4-Oxazines, e.g. morpholine
- A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
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- C07D261/00—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings
- C07D261/02—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings not condensed with other rings
- C07D261/06—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings not condensed with other rings having two or more double bonds between ring members or between ring members and non-ring members
- C07D261/10—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings not condensed with other rings having two or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D261/18—Carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen
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- C07D—HETEROCYCLIC COMPOUNDS
- C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
- C07D413/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings directly linked by a ring-member-to-ring-member bond
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- C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
- C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
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- C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
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- C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
- C07D417/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
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- C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
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- C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
- C07D487/04—Ortho-condensed systems
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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Definitions
- Protein homeostasis a balance between protein synthesis, folding, trafficking, aggregation, and degradation, referred to as protein homeostasis, utilizing sensors and networks of pathways (Sitia et al, Nature 426: 891-894, 2003; Ron et al, Nat Rev Mol Cell Biol 8: 519-529, 2007).
- the cellular maintenance of protein homeostasis, or proteostasis refers to controlling the conformation, binding interactions, location and concentration of individual proteins making up the proteome.
- Protein folding in vivo is accomplished through interactions between the folding polypeptide chain and macromolecular cellular components, including multiple classes of chaperones and folding enzymes, which minimize aggregation (Wiseman et al, Cell 131: 809-821, 2007). Whether a given protein folds in a certain cell type depends on the distribution, concentration, and subcellular localization of chaperones, folding enzymes, metabolites and the like (Wiseman et al).
- Cystic fibrosis and other maladies of protein misfolding arise as a result of an imbalance in the capacity of the protein homeostasis (proteostasis) environment to handle the reduced energetic stability of misfolded, mutated proteins that are critical for normal physiology (Balch et al, Science 319, 916-9 (2008); Powers, et al, Annu Rev Biochem 78, 959-91 (2009); Hutt et al, FEB S Lett 583, 2639-46 (2009)).
- Cystic Fibrosis is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene which encodes a multi-membrane spanning epithelial chloride channel (Riordan et al., Annu Rev Biochem 11, 701-26 (2008)). Approximately ninety percent of patients have a deletion of phenylalanine (Phe) 508 (AF508) on at least one allele. This mutation results in disruption of the energetics of the protein fold leading to degradation of CFTR in the endoplasmic reticulum (ER).
- CFTR cystic fibrosis transmembrane conductance regulator
- the AF508 mutation is thus associated with defective folding and trafficking, as well as enhanced degradation of the mutant CFTR protein (Qu et al, J Biol Chem 111, 15739-44 (1997)).
- the loss of a functional CFTR channel at the plasma membrane disrupts ionic homeostasis (CF, Na + , HCO 3 " ) and airway surface hydration leading to reduced lung function (Riordan et al).
- Reduced periciliary liquid volume and increased mucus viscosity impede mucociliary clearance resulting in chronic infection and inflammation, phenotypic hallmarks of CF disease (Boucher, J Intern Med 261, 5-16 (2007)).
- AF508 CFTR also impacts the normal function of additional organs (pancreas, intestine, gall bladder), suggesting that the loss-of- function impacts multiple downstream pathways that will require correction.
- cystic fibrosis mutations in the CFTR gene and/or the activity of the CFTR channel has also been implicated in other conditions, including for example, congenital bilateral absence of vas deferens (CBAVD), acute, recurrent, or chronic pancreatitis, disseminated bronchiectasis, asthma, allergic pulmonary aspergillosis, smoking- related lung diseases, such as chronic obstructive pulmonary disease (COPD), dry eye disease, Sjogren's syndrome and chronic sinusitis, (Sloane et al. (2012), PLoS ONE 7(6): e39809.doi: 10.1371/journal. pone.0039809; Bombieri et al.
- CBAVD congenital bilateral absence of vas deferens
- COPD chronic obstructive pulmonary disease
- COPD chronic obstructive pulmonary disease
- the present invention is based, in part, on the discovery that compounds having the Formula (I) affect cystic fibrosis transmembrane conductance regulator (CFTR) activity as measured in human bronchial epithelial (hBE) cells.
- CFTR cystic fibrosis transmembrane conductance regulator
- the present invention is directed to a method of modulating cystic fibrosis transmembrane conductance regulator (CFTR) activity in a subject in need thereof comprising administering to said subject an effective amount of a compound having the Formula (I):
- CFTR cystic fibrosis transmembrane conductance regulator
- Ri is selected from the group consisting of:
- R2 is selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C2-C1 0 alkenyl, optionally substituted C2-C1 0 alkynyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, halo, OR c , NRdRd, C(0)OR c , N0 2 , CN, C(0)Rc, C(0)C(0)R c , C(0)NR d Rd, NR d C(0)R c , NRdS(0) n Rc, N(R d )(COOR c ), NR d C(0)C(0)R c , NRdC(0)NR d Rd,
- R 3 is selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C2-C1 0 alkenyl, optionally substituted C2-C1 0 alkynyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, halo, OR c , NRdRd, C(0)OR c , N0 2 , CN, C(0)Rc, C(0)C(0)R c , C(0)NR d Rd, NR d C(0)R c , NRdS(0) n Rc, N(R d )(COOR c ), NR d C(0)C(0)R c , NRdC(0)NR d Rd,
- R2 and R 3 can be taken together with the carbon atoms to which they are attached to form a fused, optionally substituted 3 to 12 membered cyclic group selected from the group consisting of optionally substituted C3-C12 cycloalkenyl, optionally substituted heterocyclic, optionally substituted aryl and optionally substituted heteroaryl;
- Rte is selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C2-C1 0 alkenyl, optionally substituted C2-C1 0 alkynyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, halo, OR c , S(0) n R c , NRdRd, C(0)OR c , N0 2 , CN, C(0)Rc, C(0)C(0)R c , C(0)NR d R d , NRdC(0)Rc, NR d S(0)Rc, N(R d )(COOR c ), NR d C(0)C(0)Rc, NRdC(0)NR d R d , NRdS(0)nRdRd, NR d S(0)nRc, S(0)NRdRd, OC(0)OR c ,
- R4b is selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C2-C1 0 alkenyl, optionally substituted C2-C1 0 alkynyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, optionally substituted heterocyclic and optionally substituted heteroaryl;
- R a is selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C2-C1 0 alkenyl, optionally substituted C2-C1 0 alkynyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted heterocyclic, optionally substituted aryl, optionally substituted heteroaryl, C(0)OR c , C(0)Rc, C(0)C(0)Rc and S(0) n Rc;
- R a and the nitrogen atom to which it is attached is taken together with an adjacent C(Rbi)(Rbi) or C(Rb2)(Rb2) to form an optionally substituted, 4- to 12-membered heterocyclic ring containing one or more ring nitrogen atoms, wherein said heterocyclic ring optionally contains one or more ring heteroatoms selected from oxygen and sulfur;
- Each Rbi and Rb2 is independently selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C2-C1 0 alkenyl, optionally substituted C2-C1 0 alkynyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3- C12 cycloalkenyl, optionally substituted heterocyclic, optionally substituted aryl, optionally substituted heteroaryl, halo, ORc, NRaRj, C(0)ORc, N0 2 , CN, C(0)R c , C(0)C(0)Rc, C(0)NR d R d , NRdC(0)Rc, NR d S(0) n Rc, N(R d )(COORc), NR d C(0)C(0)Rc, NRdC(0)NR d R d , NR d S(0) n NR d Rd, NR d S(0) n Rc, S
- Each R c is independently selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C2-C1 0 alkenyl, optionally substituted C2-C1 0 alkynyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted heterocyclic, optionally substituted aryl and optionally substituted heteroaryl;
- Y is selected from the group consisting of S(0) n ,, ⁇ 3 ⁇ 4, NR d S(0) n , NR d S(0) n NR d , NR d C(0), NRdC(0)0, RdC(0)C(0), NR ⁇ iC(0)NR d , S(0) n NR d , and O;
- Each R d is independently selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C2-C1 0 alkenyl, optionally substituted C2-C1 0 alkynyl, optionally substituted C1-C1 0 alkoxy, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted heterocyclic, optionally substituted aryl and optionally substituted heteroaryl; or two geminal 3 ⁇ 4 groups are taken together with the nitrogen atom to which they are attached to form an optionally substituted heterocyclic or an optionally substituted heteroaryl;
- k is 0 or 1 ;
- n 0, 1, 2, 3, 4, or 5;
- each n is independently 0, 1 or 2.
- the CFTR activity is enhanced. In additional embodiments, the activity of a mutant CFTR is enhanced. In some aspects, the mutant CFTR is AF508 CFTR.
- the invention is directed to treating a subject suffering from a condition associated with CFTR activity comprising administering an effective amount of a compound of Formula (I).
- the invention encompasses a method of treating a subject suffering from a disease associated with decreased or deficient CFTR activity.
- the subject is suffering from cystic fibrosis.
- the invention is directed to a method of treating a subject suffering from a disease that can be ameliorated by suppressing CFTR activity.
- the subject is suffering from a secretory diarrhea or polycystic kidney disease.
- the present invention also encompasses an enantiomerically pure compound selected from (S)-5-phenyl-N-((tetrahydrofuran-2-yl)methyl)isoxazole-3-carboxamide (Compound 2) and (R)-5-phenyl-N-((tetrahydrofuran-2-yl)methyl)isoxazole-3-carboxamide (Compound 3).
- Compound 2 -5-phenyl-N-((tetrahydrofuran-2-yl)methyl)isoxazole-3-carboxamide
- Compound 3 The chemical structures of these compounds are shown below:
- the invention is directed to Compounds 20, 90, 92, 1 15,8, 194, 195, 197, 198, 226, 230, 336, 349 and 376 shown in the Table below:
- a and an are meant to include one or more unless otherwise specified.
- a cell encompasses both a single cell and a combination of two or more cells.
- the present invention is directed to methods of modulating CFTR activity in a subject in need thereof comprising administering an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, prodrug or solvate thereof.
- the invention also encompasses methods of treating a condition associated with CFTR activity or a disease associated with a dysfunction of proteostasis comprising administering to a subject an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt, prodrug or solvate thereof.
- the compound has the Formula (I), wherein Ri is:
- the compound has the Formula (I), wherein Ri is:
- the compound has the Formula (I), wherein Ri is:
- the compound has the Formula (I), wherein Ri is
- the compound has the Formula (I), wherein Ri is
- the compound has the Formula (I) and m is 0, 1, 2, 3, 4 or 5. In additional aspects, the compound has the Formula (I) and m is 0, 1 or 2. In yet additional aspects, the compound has the Formula (I) and k is 1 and m is 0, 1 or 2.
- the compound has the Formula (I), wherein R 3 is hydrogen or optionally substituted Ci-Cio alkyl. In additional embodiments, R3 is hydrogen.
- the compound has the Formula (I), wherein R a is hydrogen or optionally substituted C1-C4 alkyl. In yet other aspects, R a is hydrogen.
- the compound has the Formula (I), wherein each of RM and R,2 is independently selected from hydrogen, OR e , and optionally substituted C1-C10 alkyl, wherein Re is hydrogen or optionally substituted C1-C10 alkyl.
- the compound has the Formula (I), wherein R 2 is selected from the group consisting of optionally substituted C1-C1 0 alkyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, optionally substituted heterocyclic and optionally substituted heteroaryl.
- R2 is selected from the group consisting of optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, optionally substituted heterocyclic and optionally substituted heteroaryl.
- R2 is optionally substituted aryl.
- R2 is optionally substituted phenyl. In certain embodiments, R2 is unsubstituted phenyl. In some embodiments, R2 is phenyl with a substitution at the para-position. In yet other aspects, R2 is optionally substituted heteroaryl. In some embodiments, R2 is optionally substituted thienyl, optionally substituted furanyl or optionally substituted pyridinyl. In certain embodiments, R2 is optionally substituted thienyl.
- the compound has the Formula (I), wherein R4 a is selected from the group consisting of optionally substituted C1-C1 0 alkyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, OR c , C(0)OR c , C(0)R c , C(0)C(0)R c , C(0)NR d Rd, optionally substituted heterocyclic and optionally substituted heteroaryl.
- R ⁇ is an optionally substituted aryl, optionally substituted heterocyclic or optionally substituted heteroaryl.
- R4 a is an optionally substituted heterocyclic or optionally substituted heteroaryl.
- R4 a is cyclopentyl, tetrahydropyranyl, triazolyl, thiadiazolyl, oxazolidinonyl, tetrahydrofuranyl, oxazolinyl, piperazinyl or morpholinyl, each optionally substituted.
- R4 a is 2-tetrahydrofuranyl or N- morpholinyl, each optionally substituted.
- R4 a is N-methyl piperazinyl.
- R4 a is an optionally substituted heteroaryl containing one or more ring nitrogen atoms.
- R4 a is selected from the group consisting of furanyl, pyridinyl, pyrazinyl, pyrazolyl, imidazolyl, isoxazolyl, triazolyl, thiazolyl, oxadiazolyl, thienyl, and benzimidazolyl, each optionally substituted.
- R4 a is optionally substituted 2-furanyl.
- R4 a is C(0)NR d R d .
- the compound has the Formula (I) and k is 0. In yet an additional embodiment, k is 0 and R4 a is an optionally substituted heterocyclic or an optionally substituted heteroaryl.
- the compound has the Formula (I), wherein Ri is
- Y is selected from the group consisting of S, S(0)2 or S(0)2 Rj, O and NRj.
- RH is selected from the group consisting of hydrogen, optionally substituted C1-C1 0 alkyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, optionally substituted heteroaryl and optionally substituted heterocyclic.
- R-n is optionally substituted Ci-Cio alkyl, optionally substituted C3-C12 cycloalkyl, optionally substituted C3-C12 cycloalkenyl, optionally substituted aryl, optionally substituted heterocyclic and optionally substituted heteroaryl.
- R4b is an optionally substituted heterocyclic or optionally substituted heteroaryl.
- RH is tetrahydropyranyl, tetrahydrofuranyl, or oxazolidinyl, each optionally substituted. In certain aspects, RH, is optionally substituted 2-tetrahydrofuranyl.
- RH is an optionally substituted heteroaryl.
- R4b is selected from the group consisting of furanyl, pyridinyl, pyrazinyl, pyrazolyl, imidazolyl, isoxazolyl, triazolyl, thiazolyl, oxadiazolyl, thienyl, thiadiazolyl, and
- RH is optionally substituted furanyl or optionally substitued imidazolyl.
- RH is a Ci- C 4 alkyl substituted with an optionally substituted heterocyclic or an optionally substituted heteroaryl, wherein said C1-C4 alkyl is optionally further substituted.
- RH is a methyl or ethyl substituted with an optionally substituted heterocyclic or an optionally substituted heteroaryl, wherein said methyl or ethyl is optionally further substituted.
- Y is S and S(0)2.
- Y is S or S(0)2 and R4b is optionally substituted heterocyclic, optionally substituted heteroaryl, or Ci- C 4 alkyl substituted with an optionally substituted heterocyclic or an optionally substituted heteroaryl, wherein said C1-C4 alkyl is optionally further substituted.
- Y is O.
- Y is O and RH, is optionally substituted C1-C10 alkyl, optionally substituted heterocyclic or optionally substituted heteroaryl.
- Y is O and RH, is optionally substituted C1-C4 alkyl.
- the compound has the Formula (I), wherein R 2 is optionally substituted phenyl and R ⁇ is an optionally substituted heterocyclic or optionally substituted heteroaryl.
- R2 is optionally substituted phenyl
- R4 a is an optionally substituted heterocyclic or optionally substituted heteroaryl
- R 3 is hydrogen and R a is hydrogen or optionally substituted C1-C4 alkyl.
- Rbi is independently selected from hydrogen, ORg, and optionally substituted C1-C1 0 alkyl, wherein R e is C1-C1 0 alkyl.
- the compound has the Formula (I), wherein R2 is unsubstituted phenyl and R ⁇ is an optionally substituted heterocyclic or optionally substituted heteroaryl.
- R2 is unsubstituted phenyl
- R ⁇ is an optionally substituted heterocyclic or optionally substituted heteroaryl
- R 3 is hydrogen
- R a is hydrogen or optionally substituted C1-C4 alkyl.
- RM is independently selected from hydrogen, ORe, and C1-C10 alkyl, wherein R e is C1-C10 alkyl.
- the compound has the Formula (I), wherein R a and the nitrogen atom to which it is attached is taken together with the adjacent C(Rbi)(Rbi) or C(Rb2)(Rb2) to form an optionally substituted, 4- to 12-membered heterocyclic ring containing one or more ring nitrogen atoms, wherein said heterocyclic ring optionally contains one or more ring heteroatoms selected from oxygen and sulfur.
- R2 is an optionally substituted aryl, for example, optionally substituted phenyl.
- R ⁇ is selected from the group consisting of hydrogen, optionally substituted C1-C10 alkyl, ORe, C(0)NRd, optionally substituted heteroaryl, and optionally substituted heterocyclic, wherein Re is hydrogen or C1-C10 alkyl.
- the invention additionally encompasses an enantiomerically pure compound having the structure below:
- the invention also encompasses a compound selected from those shown below
- the invention is a pharmaceutical composition comprising a pharmaceutically acceptable carrier and enantiomerically pure Compound 2. In additional embodiments, the invention is a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an enantiomerically pure Compound 3.
- the invention is a pharmaceutical composition
- a pharmaceutical composition comprising a compound selected from the group consisting of Compound 20, 90, 92, 115, 135, 188, 194, 195, 197, 198, 226, 230, 336, 349 and 376, and a pharmaceutically acceptable carrier.
- R2 is optionally substituted heteroaryl and in some embodiments
- R4 a is optionally substituted heterocyclic or optionally substituted heteroaryl.
- the invention thus encompasses compound of Formula (I) wherein R 2 is optionally substituted heteroaryl and R ⁇ is optionally substituted heterocyclic or optionally substituted heteroaryl.
- alkyl refers to both branched and straight-chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms; for example, "C1-C1 0 alkyl” denotes alkyl having 1 to 10 carbon atoms.
- alkyl examples include, but are not limited to, methyl, ethyl, n-propyl, i-propyl, n-butyl, i- butyl, sec -butyl, t-butyl, n-pentyl, n-hexyl, 2-methylbutyl, 2-methylpentyl, 2-ethylbutyl, 3- methylpentyl, and 4-methylpentyl.
- alkenyl refers to both straight and branched-chain moieties having the specified number of carbon atoms and having at least one carbon-carbon double bond.
- alkynyl refers to both straight and branched-chain moieties having the specified number or carbon atoms and having at least one carbon-carbon triple bond.
- cycloalkyl refers to cyclic alkyl moieties having 3 or more carbon atoms.
- examples of cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and adamantyl.
- cycloalkenyl refers to cyclic alkenyl moieties having 3 or more carbon atoms.
- cycloalkynyl refers to cyclic alkynyl moieties having 5 or more carbon atoms.
- heterocyclic encompasses heterocycloalkyl, heterocycloalkenyl, heterobicycloalkyl, heterobicycloalkenyl, heteropolycycloalkyl, heteropolycycloalkenyl, and the like.
- Heterocycloalkyl refers to cycloalkyl groups containing one or more heteroatoms (O, S, or N) within the ring.
- Heterocycloalkenyl as used herein refers to cycloalkenyl groups containing one or more heteroatoms (O, S or N) within the ring.
- Heterobicycloalkyl refers to bicycloalkyl groups containing one or more heteroatoms (O, S or N) within a ring.
- Heterobicycloalkenyl refers to bicycloalkenyl groups containing one or more heteroatoms (O, S or N) within a ring.
- Cycloalkyl, cycloalkenyl, heterocyclic, groups also include groups similar to those described above for each of these respective categories, but which are substituted with one or more oxo moieties.
- aryl refers to mono- or polycyclic aromatic carbocyclic ring systems.
- a polycyclic aryl is a polycyclic ring system that comprises at least one aromatic ring.
- Polycyclic aryls can comprise fused rings, covalently attached rings or a combination thereof.
- aryl embraces aromatic radicals, such as, phenyl, naphthyl, indenyl, tetrahydronaphthyl, and indanyl.
- An aryl group may be substituted or unsubstituted.
- the aryl is a C4-C10 aryl.
- heteroaryl refers to aromatic carbocyclic groups containing one or more heteroatoms (O, S, or N) within a ring.
- a heteroaryl group can be monocyclic or polycyclic.
- a heteroaryl group may additionally be substituted or
- heteroaryl groups of this invention can also include ring systems substituted with one or more oxo moieties.
- a polycyclic heteroaryl can comprise fused rings, covalently attached rings or a combination thereof.
- a polycyclic heteroaryl is a polycyclic ring system that comprises at least one aromatic ring containing one or more heteroatoms within a ring.
- Polycyclic aryls can comprise fused rings, covalently attached rings or a combination thereof.
- heteroaryl groups include, but are not limited to, pyridinyl, pyridazinyl, imidazolyl, pyrimidinyl, pyrazolyl, triazolyl, pyrazinyl, quinolyl, isoquinolyl, tetrazolyl, furyl, thienyl, isoxazolyl, thiazolyl, oxazolyl, isothiazolyl, pyrrolyl, quinolinyl, isoquinolinyl, indolyl, benzimidazolyl, benzofuranyl, cinnolinyl, indazolyl, indolizinyl, phthalazinyl, triazinyl, isoindolyl, purinyl, oxadiazolyl, thiadiazolyl, furazanyl, benzofurazanyl, benzothiophenyl, benzotriazolyl, benzothiazolyl, benzo
- heteroaryl groups may be C-attached or heteroatom-attached (where such is possible).
- a group derived from pyrrole may be pyrrol- 1-yl (N-attached) or pyrrol-3-yl (C-attached).
- the heteroaryl is 4- to 10-membered heteroaryl.
- substituted refers to substitution by independent replacement of one, two, or three or more of the hydrogen atoms with substituents including, but not limited to, -Ci- Ci2 alkyl, -C2-C12 alkenyl, -C2-C12 alkynyl, -C3-C12 cycloalkyl, -C3-C12 cycloalkenyl, C3-C12 cycloalkynyl, -heterocyclic, -F, -CI, -Br, -I, -OH, -N0 2 , -N 3 , -CN, -NH 2 , oxo, thioxo, -NHR X , -NR X R X , dialkylamino, -diarylamino, -diheteroarylamino, -OR x , -C(0)R y , -C(0)C(0)R y ,
- haloalkyl refers to an alkyl group having 1 to (2n+l) substituent(s) independently selected from F, CI, Br or I, where n is the maximum number of carbon atoms in the alkyl group.
- H is the symbol for hydrogen
- N is the symbol for nitrogen
- S is the symbol for sulfur
- O is the symbol for oxygen
- Me is an abbreviation for methyl.
- Non-limiting examples of optionally substituted aryl are phenyl, substituted phenyl, napthyl and substituted naphthyl.
- Certain of the compounds described herein contain one or more asymmetric centers and may thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that may be defined, in terms of absolute stereochemistry, as (R)- or (S)-.
- the present invention is meant to include all such possible isomers, including racemic mixtures, optically pure forms and intermediate mixtures.
- Optically active (R)- and (S)-isomers may be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques.
- “Isomers” are different compounds that have the same molecular formula.
- “Stereoisomers” are isomers that differ only in the way the atoms are arranged in space.
- “Enantiomers” are a pair of stereoisomers that are non-superimposable mirror images of each other.
- a 1 : 1 mixture of a pair of enantiomers is a “racemic” mixture.
- the term “( ⁇ )” is used to designate a racemic mixture where appropriate.
- “Diastereoisomers” are stereoisomers that have at least two asymmetric atoms, but which are not mirror-images of each other. The absolute stereochemistry is specified according to the Cahn-Ingold-Prelog R— S system.
- stereochemistry at each chiral carbon may be specified by either R or S.
- Resolved compounds whose absolute configuration is unknown can be designated (+) or (-) depending on the direction (dextro- or levorotatory) which they rotate plane polarized light at the wavelength of the sodium D line.
- the compounds described herein contain olefinic double bonds or other centers of geometric asymmetry, and unless specified otherwise, it is intended that the compounds include both E and Z geometric isomers. Likewise, all tautomeric forms are also intended to be included.
- enantiomerically pure means a stereomerically pure composition of a compound.
- a stereochemically pure composition is a composition that is free or substantially free of other stereoisomers of that compound.
- an enantiomerically pure composition of the compound is free or substantially free of the other enantiomer.
- an enantiomerically pure composition is free or substantially free of the other diastereomers.
- a compound has an R-configuration at a specific position when it is present in excess compared to the compound having an S-configuration at that position.
- a compound has an S-configuration at a specific position when it is present in excess compared to the compound having an R- configuration at that position.
- atoms making up the compounds of the present invention are intended to include isotopic forms of such atoms.
- Isotopes include those atoms having the same atomic number but different mass numbers.
- Isotopes of hydrogen include, for example, tritium and deuterium
- isotopes of carbon include, for example, 13 C and 14 C.
- the invention therefore encompasses embodiments in which one or more of the hydrogen atoms in Formula (I) are replaced with deuterium.
- the invention also encompasses embodiments wherein one or more of the carbon atoms in Formula (I) is replaced with silicon atoms.
- the invention additionally encompasses embodiment wherein one or more of the nitrogen atoms in Formula (I) are oxidized to N-oxide.
- the invention is directed to a method of modulating CFTR activity in a subject comprising administering a compound of the invention in an effective amount.
- the invention also encompasses a method of treating a patient suffering from a condition associated with CFTR activity comprising administering to said patient a therapeutically effective amount of a compound described herein.
- Treating” or “treatment” includes preventing or delaying the onset of the symptoms, complications, or biochemical indicia of a disease, alleviating or ameliorating the symptoms or arresting or inhibiting further development of the disease, condition, or disorder.
- a “subject” is an animal to be treated or in need of treatment.
- a “patient” is a human subject in need of treatment.
- an “effective amount” refers to that amount of an agent that is sufficient to achieve a desired and/or recited effect.
- an "effective amount" of the therapeutic agent that is sufficient to ameliorate of one or more symptoms of a disorder and/or prevent advancement of a disorder, cause regression of the disorder and/or to achieve a desired effect.
- modulating encompasses increasing, enhancing, inhibiting, decreasing, suppressing, and the like.
- inhibiting and “decreasing” encompass causing a net decrease by either direct or indirect means.
- increasing and “enhancing” mean to cause a net gain by either direct or indirect means.
- CFTR activity is enhanced after administration of a compound described herein when there is an increase in the CFTR activity as compared to that in the absence of the compound. In some examples, CFTR activity is suppressed after
- CFTR activity encompasses, for example, chloride channel activity of the CFTR, and/or other ion transport activity (for example, HCO 3 " transport).
- AF508 is the most prevalent mutation of CFTR which results in misfolding of the protein and impaired trafficking from the endoplasmic reticulum to the apical membrane (Dormer et al. (2001). J Cell Sci 114, 4073-4081 ; http://www.genet.sickkids.on.ca/app).
- An enhancement or suppression of CFTR activity can be measured, for example, using literature described methods, including for example, Ussing chamber assays , patch clamp assays, and hBE Ieq assay (Devor et al. (2000), Am J Physiol Cell Physiol 279(2): C461-79; Dousmanis et al. (2002), J Gen Physiol 119(6): 545-59; Bruscia et al. (2005), PNAS 103(8): 2965-2971).
- the invention also encompasses a method of treating cystic fibrosis.
- the present invention can also be used to treat other conditions associated with CFTR activity, including conditions associated with deficient CFTR activity and conditions that can be ameliorated by decreasing CFTR activity.
- the invention is directed to a method of treating a condition associated with deficient or decreased CFTR activity comprising administering an effective amount of a compound of Formula (I) that enhances CFTR activity.
- conditions associated with deficient CFTR activity are cystic fibrosis, congenital bilateral absence of vas deferens (CBAVD), acute, recurrent, or chronic pancreatitis, disseminated bronchiectasis, asthma, allergic pulmonary aspergillosis, smoking-related lung diseases, such as chronic obstructive pulmonary disease (COPD), chronic sinusitis, dry eye disease, protein C deficiency, ⁇ -lipoproteinemia, lysosomal storage disease, type 1 chylomicronemia, mild pulmonary disease, lipid processing deficiencies, type 1 hereditary angioedema, coagulation- fibrinolyis, hereditary hemochromatosis, CFTR-related metabolic syndrome, chronic bronchitis, constipation, pan
- COPD chronic ob
- the invention encompasses methods of treating conditions that can be ameliorated by decreasing CFTR activity comprising administering an effective amount of a compound of Formula (I) that suppresses CFTR activity.
- Non- limiting examples of conditions that can be ameliorated by suppressing CFTR activity are cholera and other secretory diarrheas, and polycystic kidney disease.
- the methods of the invention further comprise administering an additional therapeutic agent.
- the invention encompasses a method of administering a compound of Formula (I), or a compound described herein, and at least one additional therapeutic agent.
- the invention is directed to a method comprising administering a compound of Formula (I), or a compound described herein, and at least two additional thereapeutic agents.
- Additional therapeutic agents include, for example, mucolytic agents, bronchodilators, antibiotics, anti-infective agents, antiinflammatory agents, ion channel modulating agents, therapeutic agents used in gene therapy, CFTR correctors, and CFTR potentiators, or other agents that modulates CFTR activity.
- At least one additional therapeutic agent is selected from the group consisting of a CFTR corrector and a CFTR potentiator.
- CFTR correctors and potentiators are VX-770 (Ivacaftor), VX-809 (3-(6-(l-(2,2- difluorobenzo[d][l,3]dioxol-5-yl)cyclopropanecarboxamido)-3-methylpyridin-2-yl)benzoic acid, VX-661 ( 1 -(2,2-difluoro- 1 ,3 -benzodioxol-5 -yl)-N- [ 1 - [(2R)-2,3 -dihydroxypropyl] -6- fluoro-2-(2-hydroxy-l, l-dimethylethyl)-lH-indol-5-yl]- cyclopropanecarboxamide), VX- 983, and Ataluren (P
- Non- limiting examples of anti-inflammatory agents are N6022 (3-(5-(4-(lH-imidazol-l-yl) phenyl)- l-(4-carbamoyl-2-methylphenyl)- 1 H-pyrrol-2-yl) propanoic acid), and N91 115.
- the invention encompasses administration of pharmaceutically acceptable salts of the compounds described herein.
- the invention is directed to use of pharmaceutically acceptable salts of compounds of the invention and pharmaceutical compositions thereof.
- a "pharmaceutically acceptable salt” includes an ionic bond- containing product of the reaction between the disclosed compound with either an acid or a base, suitable for administering to a subject.
- Pharmaceutically acceptable salts are well known in the art and are described, for example, in Berge et al. (1977), Pharmaceutical Salts. Journal of Pharmaceutical Sciences, 69(1): 1-19, the contents of which are herein
- a non-limiting example of a pharmaceutically acceptable salt is an acid salt of a compound containing an amine or other basic group which can be obtained by reacting the compound with a suitable organic or inorganic acid.
- pharmaceutically acceptable salts also can be metallic salts including, but not limited to, sodium, magnesium, calcium, lithium and aluminum salts. Further examples of metallic salts including, but not limited to, sodium, magnesium, calcium, lithium and aluminum salts. Further examples of metallic salts including, but not limited to, sodium, magnesium, calcium, lithium and aluminum salts.
- salts include hydrochlorides, hydrobromides, sulfates, methanesulfonates, nitrates, maleates, acetates, citrates, fumarates, tartrates (e.g. (+)-tartrates, (-)-tartrates or mixtures thereof including racemic mixtures), succinates, benzoates and salts with amino acids such as glutamic acid. Salts can also be formed with suitable organic bases when the compound comprises an acid functional group such as -C(0)OH or -S0 3 H. Such bases suitable for the formation of a pharmaceutically acceptable base addition salts with compounds of the present invention include organic bases that are nontoxic and strong enough to react with the acid functional group.
- Such organic bases include amino acids such as arginine and lysine, mono-, di-, and triethanolamine, choline, mono-, di-, and trialkylamine, such as methylamine, dimethylamine, and trimethylamine, guanidine, N-benzylphenethylamine, N-methylglucosamine, N-methylpiperazine, morpholine, ethylendiamine, tris(hydroxymethyl)aminomethane and the like.
- amino acids such as arginine and lysine, mono-, di-, and triethanolamine
- choline such as methylamine, dimethylamine, and trimethylamine
- guanidine N-benzylphenethylamine, N-methylglucosamine, N-methylpiperazine, morpholine, ethylendiamine, tris(hydroxymethyl)aminomethane and the like.
- the invention also includes administration of hydrates of the compounds described herein, including, for example, solvates of the compounds described herein, pharmaceutical compositions comprising the solvates and methods of use of the solvates.
- the invention is a solvate of a compound of Formula (I) or a pharmaceutical composition thereof.
- the invention additionally includes use of clathrates of the compounds described herein, pharmaceutical compositions comprising the clathrates, and methods of use of the clathrates.
- the invention is directed to clathrates of a compound of Formula (I) or a pharmaceutical composition thereof.
- the invention includes administration of pharmaceutical compositions comprising a pharmaceutically acceptable carrier or excipient and a compound described herein.
- the compounds of Formula (I) or a pharmaceutically acceptable salt, solvate, clathrate or prodrug can be administered in pharmaceutical compositions comprising a pharmaceutically acceptable carrier or excipient.
- the excipient can be chosen based on the expected route of administration of the composition in therapeutic applications.
- the route of administration of the composition depends on the condition to be treated. For example, intravenous injection may be preferred for treatment of a systemic disorder and oral administration may be preferred to treat a gastrointestinal disorder.
- compositions to be administered can be determined by the skilled artisan without undue experimentation in conjunction with standard dose-response studies. Relevant circumstances to be considered in making those determinations include the condition or conditions to be treated, the choice of composition to be administered, the age, weight, and response of the individual patient, and the severity of the patient's symptoms.
- a pharmaceutical composition comprising a compound of Formula (I), or a pharmaceutically acceptable salt, solvate, clathrate or prodrug, can be administered by a variety of routes including, but not limited to, parenteral, oral, pulmonary, ophthalmic, nasal, rectal, vaginal, aural, topical, buccal, transdermal, intravenous, intramuscular, subcutaneous, intradermal, intraocular, intracerebral, intralymphatic, intraarticular, intrathecal and intraperitoneal.
- the compositions can also include, depending on the formulation desired, pharmaceutically- acceptable, non-toxic carriers or diluents, which are defined as vehicles commonly used to formulate pharmaceutical compositions for animal or human administration.
- the diluent is selected so as not to affect the biological activity of the pharmacologic agent or composition.
- examples of such diluents are distilled water, physiological phosphate-buffered saline, Ringer's solutions, dextrose solution, and Hank's solution.
- the pharmaceutical composition or formulation may also include other carriers, adjuvants, or nontoxic, nontherapeutic, nonimmunogenic stabilizers and the like.
- compositions can also include large, slowly metabolized macromolecules such as proteins, polysaccharides such as chitosan, polylactic acids, polyglycolic acids and copolymers (such as latex functionalized SEPHAROSETM, agarose, cellulose, and the like), polymeric amino acids, amino acid copolymers, and lipid aggregates (such as oil droplets or liposomes).
- macromolecules such as proteins, polysaccharides such as chitosan, polylactic acids, polyglycolic acids and copolymers (such as latex functionalized SEPHAROSETM, agarose, cellulose, and the like), polymeric amino acids, amino acid copolymers, and lipid aggregates (such as oil droplets or liposomes).
- compositions can be administered parenterally such as, for example, by intravenous, intramuscular, intrathecal or subcutaneous injection.
- Parenteral administration can be accomplished by incorporating a composition into a solution or suspension.
- solutions or suspensions may also include sterile diluents such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents.
- Parenteral formulations may also include antibacterial agents such as, for example, benzyl alcohol or methyl parabens, antioxidants such as, for example, ascorbic acid or sodium bisulfite and chelating agents such as EDTA.
- Buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose may also be added.
- the parenteral preparation can be enclosed in ampules, disposable syringes or multiple dose vials made of glass or plastic.
- auxiliary substances such as wetting or emulsifying agents, surfactants, pH buffering substances and the like can be present in compositions.
- Other components of pharmaceutical compositions are those of petroleum, animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, and mineral oil.
- glycols such as propylene glycol or polyethylene glycol are preferred liquid carriers, particularly for injectable solutions.
- Injectable formulations can be prepared either as liquid solutions or suspensions; solid forms suitable for solution in, or suspension in, liquid vehicles prior to injection can also be prepared.
- the preparation also can also be emulsified or encapsulated in liposomes or micro particles such as polylactide, polyglycolide, or copolymer for enhanced adjuvant effect, as discussed above [Langer, Science 249: 1527, 1990 and Hanes, Advanced Drug Delivery Reviews 28: 97-119, 1997].
- the compositions and pharmacologic agents described herein can be administered in the form of a depot injection or implant preparation which can be formulated in such a manner as to permit a sustained or pulsatile release of the active ingredient.
- Additional formulations suitable for other modes of administration include oral, intranasal, and pulmonary formulations, suppositories, transdermal applications and ocular delivery.
- binders and carriers include, for example, polyalkylene glycols or triglycerides; such suppositories can be formed from mixtures containing the active ingredient in the range of about 0.5% to about 10%, preferably about 1% to about 2%.
- Oral formulations include excipients, such as pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, and magnesium carbonate. Topical application can result in transdermal or intradermal delivery.
- Transdermal delivery can be achieved using a skin patch or using transferosomes.
- the pharmaceutical compositions can be incorporated with excipients and used in the form of tablets, troches, capsules, elixirs, suspensions, syrups, wafers, chewing gums and the like.
- Tablets, pills, capsules, troches and the like may also contain binders, excipients, disintegrating agent, lubricants, glidants, sweetening agents, and flavoring agents.
- binders include microcrystalline cellulose, gum tragacanth or gelatin.
- excipients include starch or lactose.
- disintegrating agents include alginic acid, corn starch and the like.
- lubricants include magnesium stearate or potassium stearate.
- glidant is colloidal silicon dioxide.
- sweetening agents include sucrose, saccharin and the like.
- flavoring agents include peppermint, methyl salicylate, orange flavoring and the like. Materials used in preparing these various compositions should be pharmaceutically pure and non-toxic in the amounts used. In another embodiment, the composition is administered as a tablet or a capsule.
- tablets may be coated with shellac, sugar or both.
- a syrup or elixir may contain, in addition to the active ingredient, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and a flavoring such as cherry or orange flavor, and the like.
- a pharmaceutical composition may be presented as pessaries, tampons, creams, gels, pastes, foams or spray.
- nasally administering or nasal administration includes administering the composition to the mucus membranes of the nasal passage or nasal cavity of the patient.
- pharmaceutical compositions for nasal administration of a composition include therapeutically effective amounts of the compounds prepared by well-known methods to be administered, for example, as a nasal spray, nasal drop, suspension, gel, ointment, cream or powder. Administration of the composition may also take place using a nasal tampon or nasal sponge.
- suitable formulations may include biocompatible oil, wax, gel, powder, polymer, or other liquid or solid carriers.
- Such formulations may be administered by applying directly to affected tissues, for example, a liquid formulation to treat infection of conjunctival tissue can be administered dropwise to the subject's eye, or a cream formulation can be administered to the skin.
- Rectal administration includes administering the pharmaceutical compositions into the rectum or large intestine. This can be accomplished using suppositories or enemas.
- Suppository formulations can easily be made by methods known in the art.
- suppository formulations can be prepared by heating glycerin to about 120°C, dissolving the pharmaceutical composition in the glycerin, mixing the heated glycerin after which purified water may be added, and pouring the hot mixture into a suppository mold.
- Transdermal administration includes percutaneous absorption of the composition through the skin.
- Transdermal formulations include patches, ointments, creams, gels, salves and the like.
- pulmonary will also mean to include a tissue or cavity that is contingent to the respiratory tract, in particular, the sinuses.
- an aerosol formulation containing the active agent a manual pump spray, nebulizer or pressurized metered-dose inhaler as well as dry powder formulations are contemplated.
- Suitable formulations of this type can also include other agents, such as antistatic agents, to maintain the disclosed compounds as effective aerosols.
- a drug delivery device for delivering aerosols comprises a suitable aerosol canister with a metering valve containing a pharmaceutical aerosol formulation as described and an actuator housing adapted to hold the canister and allow for drug delivery.
- the canister in the drug delivery device has a head space representing greater than about 15% of the total volume of the canister.
- the compound intended for pulmonary administration is dissolved, suspended or emulsified in a mixture of a solvent, surfactant and propellant. The mixture is maintained under pressure in a canister that has been sealed with a metering valve.
- the invention also encompasses the treatment of a condition associated with a dysfunction in proteostasis in a subject comprising administering to said subject an effective amount of a compound of Formula (I) that enhances, improves or restores proteostasis of a protein.
- Proteostasis refers to protein homeostasis. Dysfunction in protein homeostasis is a result of protein misfolding, protein aggregation, defective protein trafficking or protein degradation.
- the invention encompasses administering a compound of Formula (I) that corrects protein misfolding, reduces protein aggregation, corrects or restores protein trafficking and/or affects protein degradation for the treatment of a condition associated with a dysfunction in proteostasis.
- a compound of Formula (I) that corrects protein misfolding and/or corrects or restores protein trafficking is administered.
- the mutated or defective enzyme is the cystic fibrosis transmembrane conductance regulator (CFTR).
- CFTR cystic fibrosis transmembrane conductance regulator
- AF508 is a deletion ( ⁇ ) of three nucleotides resulting in a loss of the amino acid phenylalanine (F) at the 508th (508) position on the protein.
- mutated cystic fibrosis transmembrane conductance regulator exists in a misfolded state and is characterized by altered trafficking as compared to the wild type CFTR.
- Additional exemplary proteins of which there can be a dysfunction in proteostasis, for example that can exist in a misfolded state include, but are not limited to, glucocerebrosidase, hexosamine A,
- Protein conformational diseases encompass gain of function disorders and loss of function disorders.
- the protein conformational disease is a gain of function disorder.
- gain of function disorder is a disease characterized by increased aggregation-associated proteotoxicity. In these diseases, aggregation exceeds clearance inside and/or outside of the cell.
- Gain of function diseases include, but are not limited to, neurodegenerative diseases associated with aggregation of polyglutamine, Lewy body diseases, amyotrophic lateral sclerosis, transthyretin-associated aggregation diseases, Alzheimer's disease, Machado- Joseph disease, cerebral B-amyloid angiopathy, retinal ganglion cell degeneration, tautopathies (progressive supranuclear palsy, corticobasal degeration, frontotemporal lobar degeneration), cerebral hemorrhage with amyloidosis, Alexander disease, Serpinopathies, familial amyloidotic neuropathy, senile systemic amyloidosis, ApoAI amyloidosis, ApoAII amyloidosis, ApoAIV amyloidosis, familial amyloidosis of the Finnish type, lysoyzme amyloidosis, fibrinogen amyloidosis, dialysis amyloidosis, inclusion body
- myositis/myopathy cataracts, medullary thyroid carcinoma, cardiac atrial amyloidosis, pituitary prolactinoma, hereditary lattice corneal dystrophy, cutaneous lichen amyloidosis, corneal lactoferrin amyloidosis, corneal lactoferrin amyloidosis, pulmonary alveolar proteinosis, odontogenic tumor amyloid, seminal vesical amyloid, sickle cell disease, critical illness myopathy, von Hippel-Lindau disease, spinocerebellar ataxia 1, Angelman syndrome, giant axon neuropathy, inclusion body myopathy with Paget disease of bone, frontotemporal dementia (IBMPFD) and prion diseases.
- IBMPFD frontotemporal dementia
- Neurodegenerative diseases associated with aggregation of polyglutamine include, but are not limited to, Huntington's disease, dentatorubral and pallidoluysian atrophy, several forms of spino-cerebellar ataxia, and spinal and bulbar muscular atrophy.
- Alzheimer's disease is characterized by the formation of two types of aggregates: extracellular aggregates of ⁇ peptide and intracellular aggregates of the microtubule associated protein tau.
- Transthyretin-associated aggregation diseases include, for example, senile systemic amyloidoses and familial amyloidotic neuropathy.
- Lewy body diseases are characterized by an aggregation of a-synuclein protein and include, for example, Parkinson's disease, lewy body dementia (LBD) and multiple system atrophy (SMA).
- Prion diseases also known as transmissible spongiform encephalopathies or TSEs are
- prion diseases characterized by aggregation of prion proteins.
- exemplary human prion diseases are
- the misfolded protein is alpha- 1 anti-trypsin.
- the protein conformation disease is a loss of function disorder.
- Loss of function diseases are a group of diseases characterized by inefficient folding of a protein resulting in excessive degradation of the protein. Loss of function diseases include, for example, lysosomal storage diseases.
- Lysosomal storage diseases are a group of diseases characterized by a specific lysosomal enzyme deficiency which may occur in a variety of tissues, resulting in the build-up of molecules normally degraded by the deficient enzyme.
- the lysosomal enzyme deficiency can be in a lysosomal hydrolase or a protein involved in the lysosomal trafficking.
- Lysosomal storage diseases include, but are not limited to, aspartylglucosaminuria, Fabry's disease, Batten disease,
- Mucolipidosis III Neimann-Pick Disease (including Types A, B and C), Pompe's disease, Sandhoff disease, Sanfilippo syndrome (including Types A, B, C and D), Schindler disease, Schindler-Kanzaki disease, Sialidosis, Sly syndrome, Tay-Sach's disease and Wolman disease.
- the disease associated with a dysfunction in proteostasis is a cardiovascular disease.
- Cardiovascular diseases include, but are not limited to, coronary artery disease, myocardial infarction, stroke, restenosis and arteriosclerosis.
- Conditions associated with a dysfunction of proteostasis also include ischemic conditions, such as, ischemia/reperfusion injury, myocardial ischemia, stable angina, unstable angina, stroke, ischemic heart disease and cerebral ischemia.
- the disease associated with a dysfunction in proteostasis is diabetes and/or complications of diabetes, including, but not limited to, diabetic retinopathy, cardiomyopathy, neuropathy, nephropathy, and impaired wound healing.
- the disease associated with a dysfunction in proteostasis is an ocular disease including, but not limited to, age-related macular degeneration (AMD), diabetic macular edema (DME), diabetic retinopathy, glaucoma, cataracts, retinitis pigmentosa (RP) and dry macular degeneration.
- AMD age-related macular degeneration
- DME diabetic macular edema
- RP retinitis pigmentosa
- dry macular degeneration including, but not limited to, age-related macular degeneration (AMD), diabetic macular edema (DME), diabetic retinopathy, glaucoma, cataracts, retinitis pigmentosa (RP) and dry macular degeneration.
- the method of the invention is directed to treating a disease associated with a dysfunction in proteostasis, wherein the disease affects the respiratory system or the pancreas.
- the methods of the invention encompass treating a condition selected from the group consisting of
- hemoglobinopathies inflammatory diseases, intermediate filament diseases, drug-induced lung damage and hearing loss.
- the invention also encompasses methods for the treatment of hemoglobinopathies (such as sickle cell anemia), an inflammatory disease (such as inflammatory bowel disease, colitis, ankylosing spondylitis), intermediate filament diseases (such as non-alcoholic and alcoholic fatty liver disease) and drug induced lung damage (such as methotrexate-induced lung damage).
- the invention additionally encompasses methods for treating hearing loss, such as noise-induced hearing loss, aminoglycoside-induced hearing loss, and cisplatin-induced hearing loss.
- Additional conditions include those associated with a defect in protein trafficking and that can be treated according to methods of the invention include: PGP mutations, hERG trafficking mutations, nephrongenic diabetes insipidus mutations in the arginine-vasopressin receptor 2, persistent hyperinsulinemic hypoglycemia of infancy (PHH1) mutations in the sulfonylurea receptor 1, and alAT.
- Step 1 Synthesis of 4-(phenyl)-2, 4-dioxo-butyric acid ethyl ester: Intermediate C
- Step-2 Synthesis of 5-(phenyl)-isoxazole-2-carboxylic acid ethyl ester:
- Step-3 Synthesis of 5-(phenyl)-isoxazole-2-carboxylic acid: Intermediate F
- THF THF
- LiOH.H 2 0 3.86 g, 0.0921mole
- TLC TLC
- reaction mass was concentrated on rotary evaporator. Crude mass was diluted with water and acidified with dilute HCl. Resultant solid was filtered and dried under vacuum. Yield- 7.1 g (82%).
- Step-4 Synthesis of 5-(phenyl)-isoxazole-2-carboxylic acid amide:
- HPLC 220nm: 99.25%, 254nm: 99.82%.
- HPLC 220nm: 98.21%, 254nm: 98.96%.
- Step 1 in the scheme above can be performed as described in Murtagh et al. (2005), Novel amine- catalyzed hydroalkoxylation reactions of activated alkenes and alkynes, Chemical
- Step-2 Synthesis of (chloromethylene) dimethyl ammonium chloride (3):
- Step-3 and Step-4 Synthesis of Isopropylidene-(lH-pyrrol-3-yl)-ammonium chloride (4) followed by lH-Pyrrole-3-carbaldehyde (5):
- Steps 1, 2 and 3 can be performed as described in Arikawa et al. (2012). Discovery of a Novel Pyrrole Derivative l-[5-(2-Fluorophenyl)-l-(pyridin-3-ylsulfonyl)-lH-pyrrol-3-yl]-N- methylmethanamine Fumarate (TAK-438) as a Potassium-Competitive Acid Blocker (P-CAB). Journal of Medicinal Chemistry 55(9), 4446-4456; Morrison et al. (2009), Synthesis of Pyrrolnitrin and Related Halogenated Phenylpyrroles, Organic Letters, 2009, 11(5), 1051-1054; Purkarthofer et al.
- Reagent 6 can be synthesized as described in Peters et al. (2013), A modular synthesis of teraryl-based a-helix mimetics, Part 1 : Synthesis of core fragments with two electronically differentiated leaving groups, Chemistry - A European Journal, 19(7), 2442-2449; Aitken et al. (2006), Synthesis, thermal reactivity, and kinetics of stabilized phosphorus ylides. Part 2:
- Step-1 3-(2-Methyl-2H-pyrazol-3-yl)-acrylonitrile (2): To a stirred solution of 2-Methyl- 2H-pyrazole-3-carbaldehyde (1.00 g, 0.0099 mol) in toluene (30 mL) was added Wittig salt (3.37 g, 0.0099 mol) at room temperature. To this resulted suspension was added DBU (1.52 mL, 0.0099 mole) drop wise and heated to reflux for 3 h. After completion of reaction toluene was distilled off completely under vacuum. Resulted crude oily mass was purified on combi flash. Pure Evaporation of solvent afforded compound 2 (0.450 g, 41.32% yield) as White Solid.
- Step-2 3-(2-Methyl-2H-pyrazol-3-yl)-propylamine (3): To a stirred solution of 3-(2-
- the Wittig reagent can be purchased or synthesized as described in the following references: Kiddle et al. (2000), Microwave irradiation in organophosphorus chemistry. Part 2: Synthesis of phosphonium salts, Tetrahedron Letters, 41(9), 1339-1341; Suzanne et al. (2007), C-H Activation Reactions of Ruthenium N-Heterocyclic Carbene Complexes:
- Step-2 3-(3-Methyl-3H-imidazol-4-yl)-acrylonitrile (3) and 3-(l-Methyl-lH-imidazol-4- yl)- acrylonitrile (3A):
- Step-3 3-(3-Methyl-3H-imidazol-4-yl)-polyamine (4):
- Step-3 3-(l-Methyl-lH-imidazol-4-yl)-polyamine (4A) and Bis-[3-(l-methyl-lH- imidazol-4-yl)-Pr opyl] -amine (4B) :
- Step-1 3-Furan-3-yl-acrylonitrile: To a stirred solution of Furan-3-carbaldehyde (0.500 g, 0.0520 mol) in toluene (5 mL) was added Wittig salt (5) (1.86 g, 0.00515 mol) (Synthesized using refluxing of Chloroacetonitrile and Triphenyl phosphine in toluene) at room
- Step-2 3-Furan-3-yl-propylamine (Amine for compound 194): To a stirred solution of 3- Furan-3-yl-acrylonitrile (0.300 g, 0.00252 mol) in ethanol (5 mL) was added Raney Ni (0.5 g, 50% in water suspension) at room temperature. Reaction mixture was then stirred under 1 Atm of Hydrogen for 18 h. After completion, reaction was filtered through celite bed and washed with ethanol (5 x 2 mL). Filtrate was evaporated under vacuum. Crude mass obtained was purified using neutral aluminum oxide column chromatography. Pure compound was eluted with 5% Methanol in DCM and 1% Ammonia solution.
- the amine for compound 185 was prepared as described below or the amine can be purchased from commercial vendors such as Aldrich. Synthesis of imidazole amine prepared as in BMCL, 18 (2008), 464 - 468: Carl P Bergstrom et al.
- hBEs Primary lung epithelial cells homozygous for the Cystic Fibrosis-causing
- AF508 mutation were differentiated for a minimum of 4 weeks in an air-liquid interface on Snap Well filter plates prior to the Ussing measurements.
- Cells were apically mucus-washed for 30 minutes prior to treatment with compounds.
- the basolateral media was removed and replaced with media containing the compound of interest diluted to its final concentration from DMSO stocks.
- Treated cells were incubated at 37°C and 5%C0 2 for 24 hours. At the end of the treatment period, the cells on filters were transferred to the Ussing chamber and equilibrated for 30 minutes.
- Genistein to both chambers to potentiate AF508-CFTR channel opening.
- Genistein to both chambers to potentiate AF508-CFTR channel opening.
- CFTRinh-172 to the apical chamber to inhibit AF508-CFTR CI- conductance.
- the inhibitable current (that current that is blocked by CFTRinh-172) was measured as the specific activity of the AF508-CFTR channel, and increases in response to compound in this activity over that observed in vehicle-treated samples were identified as the correction of AF508-CFTR function imparted by the compound tested.
- VT transepithelial voltage
- GT conductance
- the baseline VT and GT values were measured for approximately 20 minutes.
- the activity data captured was the area under the curve (AUC) for the traces of the equivalent chloride current.
- the AUC was collected from the time of the forskolin/VX-770 addition until the inhibition by bumetanide addition. Correction in response to compound treatment was scored as the increase in the AUC for compound-treated samples over that of vehicle-treated samples. (++ indicates activity >25% run at 10 uM of VX-809 at 1 uM, + indicates activity 10 to ⁇ 25% run at 10 uM of VX-809 at 1 uM.
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Abstract
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-
2014
- 2014-06-25 AU AU2014302458A patent/AU2014302458A1/en not_active Abandoned
- 2014-06-25 CA CA2915975A patent/CA2915975A1/en not_active Abandoned
- 2014-06-25 MX MX2015017532A patent/MX2015017532A/en unknown
- 2014-06-25 EP EP14816975.8A patent/EP3013341A4/en not_active Withdrawn
- 2014-06-25 WO PCT/US2014/044100 patent/WO2014210159A1/en active Application Filing
- 2014-06-25 US US14/900,345 patent/US20160151335A1/en not_active Abandoned
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2015
- 2015-12-10 ZA ZA2015/09019A patent/ZA201509019B/en unknown
- 2015-12-27 IL IL243360A patent/IL243360A0/en unknown
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Also Published As
Publication number | Publication date |
---|---|
ZA201509019B (en) | 2017-08-30 |
EP3013341A4 (en) | 2017-02-08 |
AU2014302458A1 (en) | 2015-12-24 |
MX2015017532A (en) | 2016-10-26 |
CA2915975A1 (en) | 2014-12-31 |
EP3013341A1 (en) | 2016-05-04 |
IL243360A0 (en) | 2016-02-29 |
US20160151335A1 (en) | 2016-06-02 |
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