WO2014203268A3 - Method for isolation, purification and industrial scale expansion of equine adipose tissue derived mensenchymal stem cells - Google Patents

Method for isolation, purification and industrial scale expansion of equine adipose tissue derived mensenchymal stem cells Download PDF

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Publication number
WO2014203268A3
WO2014203268A3 PCT/IN2013/000583 IN2013000583W WO2014203268A3 WO 2014203268 A3 WO2014203268 A3 WO 2014203268A3 IN 2013000583 W IN2013000583 W IN 2013000583W WO 2014203268 A3 WO2014203268 A3 WO 2014203268A3
Authority
WO
WIPO (PCT)
Prior art keywords
isolation
purification
stem cells
adipose tissue
industrial scale
Prior art date
Application number
PCT/IN2013/000583
Other languages
French (fr)
Other versions
WO2014203268A2 (en
Inventor
Satish Mahadoedaro TOTEY
Manoj Kumar C REDDY
Lyle Carl FONSECA
Shashank GOWDA
Aarya HARI
Basavaraj CHOUGULE
Minita SODHI
Original Assignee
Kasiak Research Pvt. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kasiak Research Pvt. Ltd. filed Critical Kasiak Research Pvt. Ltd.
Publication of WO2014203268A2 publication Critical patent/WO2014203268A2/en
Publication of WO2014203268A3 publication Critical patent/WO2014203268A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells

Abstract

The invention relates to a method for isolation, purification and industrial scale expansion of equine adipose tissue derived mesenchymal stem cells (MSCs). The invention also relates to a method for treating and a therapeutic product for treating tendon injury, ligament injury, osteoarthritis and exercise induced pulmonary hemorrhage comprising MSCs.
PCT/IN2013/000583 2013-06-17 2013-09-26 Method for isolation, purification and industrial scale expansion of equine adipose tissue derived mensenchymal stem cells WO2014203268A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2047/MUM/2013 2013-06-17
IN2047MU2013 IN2013MU02047A (en) 2013-06-17 2013-09-26

Publications (2)

Publication Number Publication Date
WO2014203268A2 WO2014203268A2 (en) 2014-12-24
WO2014203268A3 true WO2014203268A3 (en) 2015-03-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2013/000583 WO2014203268A2 (en) 2013-06-17 2013-09-26 Method for isolation, purification and industrial scale expansion of equine adipose tissue derived mensenchymal stem cells

Country Status (2)

Country Link
IN (1) IN2013MU02047A (en)
WO (1) WO2014203268A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453202B2 (en) 2003-10-08 2016-09-27 Vet-Stem, Inc. Methods of preparing and using novel stem cell compositions and kits comprising the same
CN107828726A (en) * 2017-12-15 2018-03-23 北京焕生汇生物技术研究院 A kind of method of the fractionation of fatty mescenchymal stem cell from fat
WO2019175773A1 (en) 2018-03-12 2019-09-19 Universidade Do Porto Compositions for use in the treatment of musculoskeletal conditions and methods for producing the same leveraging the synergistic activity of two different types of mesenchymal stromal/stem cells
WO2024033462A1 (en) * 2022-08-11 2024-02-15 Boehringer Ingelheim Vetmedica Gmbh Mesenchymal stem cells for use in the treatment of insect-bite hypersensitivity in equines

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011047093A2 (en) * 2009-10-13 2011-04-21 Celavie Biosciences, Llc Stem cells for musculoskeletal tissue repair
WO2011101834A1 (en) * 2010-02-22 2011-08-25 Advanced Neuro-Science Allies Private Limited A method for obtaining mesenchymal stem cells, media, methods and composition thereof
US20110229965A1 (en) * 2009-11-27 2011-09-22 Rakhi Pal Methods of Preparing Mesenchymal Stem Cells, Compositions and Kit Thereof
US20120149098A1 (en) * 2010-12-10 2012-06-14 Davide VANNONI Extraction of process for mesenchymal stromal stem cells
US20130129688A1 (en) * 2011-11-09 2013-05-23 Allocure, Inc. Assay for the Prediction of Therapeutic Effectiveness or Potency of Mesenchymal Stem Cells, and Methods of Using Same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011047093A2 (en) * 2009-10-13 2011-04-21 Celavie Biosciences, Llc Stem cells for musculoskeletal tissue repair
US20110229965A1 (en) * 2009-11-27 2011-09-22 Rakhi Pal Methods of Preparing Mesenchymal Stem Cells, Compositions and Kit Thereof
WO2011101834A1 (en) * 2010-02-22 2011-08-25 Advanced Neuro-Science Allies Private Limited A method for obtaining mesenchymal stem cells, media, methods and composition thereof
US20120149098A1 (en) * 2010-12-10 2012-06-14 Davide VANNONI Extraction of process for mesenchymal stromal stem cells
US20130129688A1 (en) * 2011-11-09 2013-05-23 Allocure, Inc. Assay for the Prediction of Therapeutic Effectiveness or Potency of Mesenchymal Stem Cells, and Methods of Using Same

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CARVALHO ET AL.: "Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.", VET IMMUNOL IMMUNOPATHOL., vol. 132, no. 2-4, December 2009 (2009-12-01), pages 303 - 306 *
OEDAYRAJSINGH-VARMA ET AL.: "Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure.", CYTOTHERAPY., vol. 8, no. 2, 2006, pages 166 - 177 *
TUEMMERS ET AL.: "Effect of the application of stem cells for tendon injuries in sporting horses.", ARCH. MED. VET., vol. 44, 2012, pages 207 - 215 *
WELTER ET AL.: "Assessing Adipogenic Potential of Mesenchymal Stem Cells: A Rapid Three- Dimensional Culture Screening Technique.", STEM CELLS INT., vol. 2013, February 2013 (2013-02-01) *

Also Published As

Publication number Publication date
WO2014203268A2 (en) 2014-12-24
IN2013MU02047A (en) 2015-06-05

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